Carg24846 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24846
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	small heat shock protein, chloroplastic-like
Location	Carg_Chr16: 9255710 .. 9257074 (+)
RNA-Seq Expression	Carg24846
Synteny	Carg24846

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAGAACCTTCTTTCCCCTTCCCCACGCTTCTTCTTCTTCTATAAAACCCCAACTTCTCGTGCCCCTACTCCGCAATTCTTTGAACCAGTGAATTATCCCTTACGTCTCTGAGGTTCAAGGTTTCCAAATCTGCATCAATGGCTTCTTCGATCGCTCTTCGAAGGCTGGCTGCTAACTCCGCCGCTAAGCTCTTCAATCCTGTTCGCTCAGCTTCTGTTCTCCCCTCTGTCGGTCGTTCGTTCAACACTAATGCTCAGATGACCAGCTACAATGATGACGAACGCTCCGTTGACGTGGACCGCAGCTCTGACCGCTCTCTGTCTCGTTCTTATGATCGCTTTCCTGGTTTCGCAGGTAGTTGTTATCTTTGTCCTTGTTAGAGCCTTTAATTGATTATGATGCTATATTCTTCTGGAGTTTCGAACTACGTTTTCGTTAGCTTTTTGTTGATTGGTTGTGTAATACGGTTACATTTGTGGAAGTGGTTATGAAACATCCGAGTTGAATTCGAGGTTATATGTTGTGAGAGCATAGCTGAACTATTTCATCGGTCTTTGAGGAATGTCGTGATGAAATAATCTATGAATTGGCTGTTTTTTAGTGACGATCCTGCTTGCTTGTTCATGACGAATCCTGGTGAGATTACTCTGCTTGACGAGTTTCACGAATTTCGGCATGATAATGGTTATTGATTGAGTAGTCGAGTGTAAATATTTGCTTGCTTGTTGAAGTCATGAAACCATCTGGAAGTGGTTGTGACCGAGTGAGAAAGCTTGATCTGGTCACTCTGCTCGACGATTCTCTGCTACGAAATGATTTGAAATTTGTTGTGTTTCGGAGTGGATCTTGTTTATTCCAATGTTGGCATGAAATCTTCAAAAAATGAGTCTCGATTTAGCGAAGAACTTCGCTCGCTTGCTGATGGCAGATCCTTCTTTGCATTCTAAGATCTTTATCTTGGTGTTCGTGGCGAATCTTGCACTGTTCTCTCTCCTGGATGAATATCACAATGTGTCATACATTTGTTGTATTTTAGTTGTATCATTTAGTTGTTTTTAATGCGAGAAATCTTGTTCTGAACAATGCTTGGTTGTCGATTTACAGACGTGTTCGATCCGTTTTCCTCCCACACGCAGCCTAAGCCAGGTTCTGAATCTGATGGATCAGTTCATGGAGGATCCGTTATCTGGCAGCTTCACGAGGAGTAGGAGCAGGATCAAGCGGGAGGGGATGGGACGGTGAGAGAAGACGACGACGCTCTGTATCTACGAATGGACATGCCTGGACTGAGCAAAGAAGATGTGAAAGTGAGTGTGGAGCAGAACACTCTCATTATCAAAGGCGAGGCTGCTAAAGAAATCTGA

mRNA sequence

CTAGAACCTTCTTTCCCCTTCCCCACGCTTCTTCTTCTTCTATAAAACCCCAACTTCTCGTGCCCCTACTCCGCAATTCTTTGAACCAGTGAATTATCCCTTACGTCTCTGAGGTTCAAGGTTTCCAAATCTGCATCAATGGCTTCTTCGATCGCTCTTCGAAGGCTGGCTGCTAACTCCGCCGCTAAGCTCTTCAATCCTGTTCGCTCAGCTTCTGTTCTCCCCTCTGTCGGTCGTTCGTTCAACACTAATGCTCAGATGACCAGCTACAATGATGACGAACGCTCCGTTGACGTGGACCGCAGCTCTGACCGCTCTCTGTCTCGTTCTTATGATCGCTTTCCTGGTTTCGCAGACGTGTTCGATCCGTTTTCCTCCCACACGCAGCCTAAGCCAGGTTCTGAATCTGATGGATCAGTTCATGGAGGATCCGAGCAGGATCAAGCGGGAGGGGATGGGACGGTGAGAGAAGACGACGACGCTCTGTATCTACGAATGGACATGCCTGGACTGAGCAAAGAAGATGTGAAAGTGAGTGTGGAGCAGAACACTCTCATTATCAAAGGCGAGGCTGCTAAAGAAATCTGA

Coding sequence (CDS)

ATGGCTTCTTCGATCGCTCTTCGAAGGCTGGCTGCTAACTCCGCCGCTAAGCTCTTCAATCCTGTTCGCTCAGCTTCTGTTCTCCCCTCTGTCGGTCGTTCGTTCAACACTAATGCTCAGATGACCAGCTACAATGATGACGAACGCTCCGTTGACGTGGACCGCAGCTCTGACCGCTCTCTGTCTCGTTCTTATGATCGCTTTCCTGGTTTCGCAGACGTGTTCGATCCGTTTTCCTCCCACACGCAGCCTAAGCCAGGTTCTGAATCTGATGGATCAGTTCATGGAGGATCCGAGCAGGATCAAGCGGGAGGGGATGGGACGGTGAGAGAAGACGACGACGCTCTGTATCTACGAATGGACATGCCTGGACTGAGCAAAGAAGATGTGAAAGTGAGTGTGGAGCAGAACACTCTCATTATCAAAGGCGAGGCTGCTAAAGAAATCTGA

Protein sequence

MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRSLSRSYDRFPGFADVFDPFSSHTQPKPGSESDGSVHGGSEQDQAGGDGTVREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKEI

Homology

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BLAST of Carg24846 vs. NCBI nr
Match: KAG7015728.1 (Heat shock 22 kDa protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 281.2 bits (718), Expect = 5.3e-72
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 60
           MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS
Sbjct: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 60

Query: 61  LSRSYDRFPGFADVFDPFSSHTQPKPGSESDGSVHGGSEQDQAGGDGTVREDDDALYLRM 120
           LSRSYDRFPGFADVFDPFSSHTQPKPGSESDGSVHGGSEQDQAGGDGTVREDDDALYLRM
Sbjct: 61  LSRSYDRFPGFADVFDPFSSHTQPKPGSESDGSVHGGSEQDQAGGDGTVREDDDALYLRM 120

Query: 121 DMPGLSKEDVKVSVEQNTLIIKGEAAKEI 150
           DMPGLSKEDVKVSVEQNTLIIKGEAAKEI
Sbjct: 121 DMPGLSKEDVKVSVEQNTLIIKGEAAKEI 149

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BLAST of Carg24846 vs. NCBI nr
Match: KAG6577690.1 (Phosphatidylinositol/phosphatidylcholine transfer protein SFH11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 206.1 bits (523), Expect = 2.1e-49
Identity = 124/160 (77.50%), Postives = 126/160 (78.75%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 60
           MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDR SDRS
Sbjct: 355 MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRRSDRS 414

Query: 61  LSRSYDRFPGFADVFDPFS------------SHTQPKPGSESDGSVHGGSEQDQAGGDGT 120
           LSRSYDRFPGFADVFDPFS                  P   +   V  GS +   G D  
Sbjct: 415 LSRSYDRFPGFADVFDPFSPTRSLSQVLNLMDQFMEDPFLAASRGVGAGSRR---GWD-- 474

Query: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE
Sbjct: 475 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 509

[Close]

BLAST of Carg24846 vs. NCBI nr
Match: XP_022923528.1 (small heat shock protein, chloroplastic-like isoform X2 [Cucurbita moschata] >XP_023553455.1 small heat shock protein, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 206.1 bits (523), Expect = 2.1e-49
Identity = 124/160 (77.50%), Postives = 126/160 (78.75%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 60
           MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDR SDRS
Sbjct: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRRSDRS 60

Query: 61  LSRSYDRFPGFADVFDPFS------------SHTQPKPGSESDGSVHGGSEQDQAGGDGT 120
           LSRSYDRFPGFADVFDPFS                  P   +   V  GS +   G D  
Sbjct: 61  LSRSYDRFPGFADVFDPFSPTRSLSQVLNLMDQFMEDPFLAASRGVGAGSRR---GWD-- 120

Query: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE
Sbjct: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 155

[Close]

BLAST of Carg24846 vs. NCBI nr
Match: XP_022965184.1 (small heat shock protein, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 205.7 bits (522), Expect = 2.8e-49
Identity = 124/160 (77.50%), Postives = 126/160 (78.75%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 60
           MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDR SDRS
Sbjct: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRRSDRS 60

Query: 61  LSRSYDRFPGFADVFDPFS------------SHTQPKPGSESDGSVHGGSEQDQAGGDGT 120
           LSRSYDRFPGFADVFDPFS                  P   +   V  GS +   G D  
Sbjct: 61  LSRSYDRFPGFADVFDPFSPTRSLSQVLNLMDQFMEDPLLAASRGVGAGSRR---GWD-- 120

Query: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE
Sbjct: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 155

[Close]

BLAST of Carg24846 vs. NCBI nr
Match: XP_022923527.1 (small heat shock protein, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_023553454.1 small heat shock protein, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 199.5 bits (506), Expect = 2.0e-47
Identity = 123/161 (76.40%), Postives = 126/161 (78.26%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 60
           MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDR SDRS
Sbjct: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRRSDRS 60

Query: 61  LSRSYDRFPGFA-DVFDPFS------------SHTQPKPGSESDGSVHGGSEQDQAGGDG 120
           LSRSYDRFPGFA +VFDPFS                  P   +   V  GS +   G D 
Sbjct: 61  LSRSYDRFPGFAGNVFDPFSPTRSLSQVLNLMDQFMEDPFLAASRGVGAGSRR---GWD- 120

Query: 121 TVREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
            VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE
Sbjct: 121 -VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 156

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BLAST of Carg24846 vs. ExPASy Swiss-Prot
Match: Q39818 (Heat shock 22 kDa protein, mitochondrial OS=Glycine max OX=3847 GN=HSP23.9 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.6e-18
Identity = 73/161 (45.34%), Postives = 96/161 (59.63%), Query Frame = 0


Query: 1   MASSIALRRLAANS--AAKLFNPVRSASVLPSVGRSFNTNAQMTSYND--DERSVDVDRS 60
           MASS+  +R  ++S  +  L  P  SAS      RSF+TNA M  Y++  D+ S D+DR 
Sbjct: 1   MASSLIAKRFLSSSLLSRSLLRPAASAS-----HRSFDTNA-MRQYDNRADDHSTDIDRH 60

Query: 61  SDRSLSRSYDRFPGFADVFDPFSSHTQPKPGSESDGSVHGGS-----EQDQ----AGGDG 120
           S+RS   +  R   F        S ++ +PGSE DG  HG S      +D+    +G   
Sbjct: 61  SERSFPSTARRDDIFLRCVGSIFSDSEFEPGSEHDGPGHGQSVPLRVARDRSWRWSGRGW 120

Query: 121 TVREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
             RE +DAL+LR+DMPGL+KEDVK+SVEQNTLIIKGE AKE
Sbjct: 121 DARETEDALHLRVDMPGLAKEDVKISVEQNTLIIKGEGAKE 155

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BLAST of Carg24846 vs. ExPASy Swiss-Prot
Match: P11890 (Small heat shock protein, chloroplastic OS=Oxybasis rubra OX=3560 GN=HSP23 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 6.1e-15
Identity = 72/159 (45.28%), Postives = 96/159 (60.38%), Query Frame = 0


Query: 3   SSIALRRLAANS--AAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 62
           +S+ALRRLA+ +  +  +F P+       SV RSFNTNAQM    D +  +D DRS+   
Sbjct: 2   ASMALRRLASRNLVSGGIFRPL-------SVSRSFNTNAQMGRV-DHDHELD-DRSNRAP 61

Query: 63  LSRSYDRFPG--FADVFDPF-SSHTQPKPGSESD--------GSVHGGSEQDQAGGDGTV 122
           +SR  D FP   F+DVFDPF ++ +  +  +  D         +  G     + G D  V
Sbjct: 62  ISRRGD-FPASFFSDVFDPFRATRSVGQLMNLMDQLMENPFMAASRGSGRAMRRGWD--V 121

Query: 123 REDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           RED++AL L++DMPGL+KEDVKVSVE NTLIIK EA KE
Sbjct: 122 REDEEALELKVDMPGLAKEDVKVSVEDNTLIIKSEAEKE 148

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BLAST of Carg24846 vs. ExPASy Swiss-Prot
Match: P46254 (Heat shock 22 kDa protein, mitochondrial OS=Pisum sativum OX=3888 GN=HSP22 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.5e-13
Identity = 71/160 (44.38%), Postives = 91/160 (56.88%), Query Frame = 0


Query: 1   MASSIALRRLAANS--AAKLFNPVRSASVLPSVGRSFNTNA-QMTSYNDDERSVDVDRSS 60
           MASS+AL+R  ++   ++    PV S     S  RSFNTNA +    + D+R+VDV R  
Sbjct: 1   MASSLALKRFLSSGLLSSSFLRPVAS-----SASRSFNTNAMRQYDQHSDDRNVDVYR-- 60

Query: 61  DRSLSRSYDRFPGFADVFDPFSSHTQPKPGSE--------SDGSVHGGSEQDQAGGDGTV 120
             S  R+       +DVFDPFS    P+  S+        +D  V   S   + G D   
Sbjct: 61  -HSFPRTRRDDLLLSDVFDPFS---PPRSLSQVLNMVDLLTDNPVL--SAASRRGWD--A 120

Query: 121 REDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKG-EAAKE 149
           RE +DAL+LR+DMPGL KEDVK+SVEQNTL IKG E AKE
Sbjct: 121 RETEDALFLRLDMPGLGKEDVKISVEQNTLTIKGEEGAKE 145

[Close]

BLAST of Carg24846 vs. ExPASy Swiss-Prot
Match: Q96331 (23.6 kDa heat shock protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HSP23.6 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.5e-13
Identity = 69/160 (43.12%), Postives = 97/160 (60.62%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVL-PSV-GRSFNTNAQMTSYNDDERSVDVDRSSD 60
           MAS++AL+RL ++S A      RS SVL P+V  R FNTNA + SY+DD  + D      
Sbjct: 1   MASALALKRLLSSSIAP-----RSRSVLRPAVSSRLFNTNA-VRSYDDDGENGDGVDLYR 60

Query: 61  RSLSRSYDRFPGFADVFDPFS-SHTQPKPGSESDGSVHG---------GSEQDQAGGDGT 120
           RS+ R    F  F+DVFDPFS + +  +  +  D  +           G+   + G D  
Sbjct: 61  RSVPRRRGDF--FSDVFDPFSPTRSVSQVLNLMDQFMENPLLSATRGMGASGARRGWD-- 120

Query: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           ++E DDALYLR+DMPGLS+EDVK+++EQ+TL+I+GE   E
Sbjct: 121 IKEKDDALYLRIDMPGLSREDVKLALEQDTLVIRGEGKNE 150

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BLAST of Carg24846 vs. ExPASy Swiss-Prot
Match: Q9FGM9 (23.5 kDa heat shock protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HSP23.5 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 3.7e-12
Identity = 59/153 (38.56%), Postives = 85/153 (55.56%), Query Frame = 0


Query: 2   ASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRSL 61
           +S++ALRRL ++S   +   +R+   + +  R FNTNA   +Y D    VD +  S+R +
Sbjct: 4   SSALALRRLLSSSTVAVPRALRAVRPVAASSRLFNTNA-ARNYED---GVDRNHHSNRHV 63

Query: 62  SR-SYDRFPGFADVFDPFSSHTQPKPGSESDGSVHGGSEQDQAGGDG-----TVREDDDA 121
           SR   D F    D F P  S +Q     +    +   S     G  G      V+E DDA
Sbjct: 64  SRHGGDFFSHILDPFTPTRSLSQMLNFMDQVSEIPLVSATRGMGASGVRRGWNVKEKDDA 123

Query: 122 LYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           L+LR+DMPGLS+EDVK+++EQNTL+I+GE   E
Sbjct: 124 LHLRIDMPGLSREDVKLALEQNTLVIRGEGETE 152

[Close]

BLAST of Carg24846 vs. ExPASy TrEMBL
Match: A0A6J1E9W1 (small heat shock protein, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431197 PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.0e-49
Identity = 124/160 (77.50%), Postives = 126/160 (78.75%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 60
           MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDR SDRS
Sbjct: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRRSDRS 60

Query: 61  LSRSYDRFPGFADVFDPFS------------SHTQPKPGSESDGSVHGGSEQDQAGGDGT 120
           LSRSYDRFPGFADVFDPFS                  P   +   V  GS +   G D  
Sbjct: 61  LSRSYDRFPGFADVFDPFSPTRSLSQVLNLMDQFMEDPFLAASRGVGAGSRR---GWD-- 120

Query: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE
Sbjct: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 155

[Close]

BLAST of Carg24846 vs. ExPASy TrEMBL
Match: A0A6J1HQA7 (small heat shock protein, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465116 PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.4e-49
Identity = 124/160 (77.50%), Postives = 126/160 (78.75%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 60
           MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDR SDRS
Sbjct: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRRSDRS 60

Query: 61  LSRSYDRFPGFADVFDPFS------------SHTQPKPGSESDGSVHGGSEQDQAGGDGT 120
           LSRSYDRFPGFADVFDPFS                  P   +   V  GS +   G D  
Sbjct: 61  LSRSYDRFPGFADVFDPFSPTRSLSQVLNLMDQFMEDPLLAASRGVGAGSRR---GWD-- 120

Query: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE
Sbjct: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 155

[Close]

BLAST of Carg24846 vs. ExPASy TrEMBL
Match: A0A6J1E6C8 (small heat shock protein, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431197 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 9.7e-48
Identity = 123/161 (76.40%), Postives = 126/161 (78.26%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 60
           MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDR SDRS
Sbjct: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRRSDRS 60

Query: 61  LSRSYDRFPGFA-DVFDPFS------------SHTQPKPGSESDGSVHGGSEQDQAGGDG 120
           LSRSYDRFPGFA +VFDPFS                  P   +   V  GS +   G D 
Sbjct: 61  LSRSYDRFPGFAGNVFDPFSPTRSLSQVLNLMDQFMEDPFLAASRGVGAGSRR---GWD- 120

Query: 121 TVREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
            VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE
Sbjct: 121 -VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 156

[Close]

BLAST of Carg24846 vs. ExPASy TrEMBL
Match: A0A6J1HN48 (small heat shock protein, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465116 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.3e-47
Identity = 123/161 (76.40%), Postives = 126/161 (78.26%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 60
           MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDR SDRS
Sbjct: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRRSDRS 60

Query: 61  LSRSYDRFPGFA-DVFDPFS------------SHTQPKPGSESDGSVHGGSEQDQAGGDG 120
           LSRSYDRFPGFA +VFDPFS                  P   +   V  GS +   G D 
Sbjct: 61  LSRSYDRFPGFAGNVFDPFSPTRSLSQVLNLMDQFMEDPLLAASRGVGAGSRR---GWD- 120

Query: 121 TVREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
            VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE
Sbjct: 121 -VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 156

[Close]

BLAST of Carg24846 vs. ExPASy TrEMBL
Match: H6TB40 (HSP23.5 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.7e-45
Identity = 116/160 (72.50%), Postives = 124/160 (77.50%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRS 60
           MASSIALRRLAA+SAAKLF+PVRSASVLPSVGRSFNTNAQMT+Y DD+RSVDVDR SDRS
Sbjct: 1   MASSIALRRLAASSAAKLFSPVRSASVLPSVGRSFNTNAQMTNYADDDRSVDVDRGSDRS 60

Query: 61  LSRSYDRFPGFADVFDPFS------------SHTQPKPGSESDGSVHGGSEQDQAGGDGT 120
           +SRS DRFPGFADVFDPFS                  P   +   V  GS +   G D  
Sbjct: 61  ISRSRDRFPGFADVFDPFSPTRSLSQVLNLMDQLMEDPFLAASRGVGAGSRR---GWD-- 120

Query: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           VREDD+ALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE
Sbjct: 121 VREDDNALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 155

[Close]

BLAST of Carg24846 vs. TAIR 10
Match: AT4G25200.1 (mitochondrion-localized small heat shock protein 23.6 )

HSP 1 Score: 77.4 bits (189), Expect = 1.1e-14
Identity = 69/160 (43.12%), Postives = 97/160 (60.62%), Query Frame = 0


Query: 1   MASSIALRRLAANSAAKLFNPVRSASVL-PSV-GRSFNTNAQMTSYNDDERSVDVDRSSD 60
           MAS++AL+RL ++S A      RS SVL P+V  R FNTNA + SY+DD  + D      
Sbjct: 1   MASALALKRLLSSSIAP-----RSRSVLRPAVSSRLFNTNA-VRSYDDDGENGDGVDLYR 60

Query: 61  RSLSRSYDRFPGFADVFDPFS-SHTQPKPGSESDGSVHG---------GSEQDQAGGDGT 120
           RS+ R    F  F+DVFDPFS + +  +  +  D  +           G+   + G D  
Sbjct: 61  RSVPRRRGDF--FSDVFDPFSPTRSVSQVLNLMDQFMENPLLSATRGMGASGARRGWD-- 120

Query: 121 VREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           ++E DDALYLR+DMPGLS+EDVK+++EQ+TL+I+GE   E
Sbjct: 121 IKEKDDALYLRIDMPGLSREDVKLALEQDTLVIRGEGKNE 150

[Close]

BLAST of Carg24846 vs. TAIR 10
Match: AT5G51440.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 2.6e-13
Identity = 59/153 (38.56%), Postives = 85/153 (55.56%), Query Frame = 0


Query: 2   ASSIALRRLAANSAAKLFNPVRSASVLPSVGRSFNTNAQMTSYNDDERSVDVDRSSDRSL 61
           +S++ALRRL ++S   +   +R+   + +  R FNTNA   +Y D    VD +  S+R +
Sbjct: 4   SSALALRRLLSSSTVAVPRALRAVRPVAASSRLFNTNA-ARNYED---GVDRNHHSNRHV 63

Query: 62  SR-SYDRFPGFADVFDPFSSHTQPKPGSESDGSVHGGSEQDQAGGDG-----TVREDDDA 121
           SR   D F    D F P  S +Q     +    +   S     G  G      V+E DDA
Sbjct: 64  SRHGGDFFSHILDPFTPTRSLSQMLNFMDQVSEIPLVSATRGMGASGVRRGWNVKEKDDA 123

Query: 122 LYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           L+LR+DMPGLS+EDVK+++EQNTL+I+GE   E
Sbjct: 124 LHLRIDMPGLSREDVKLALEQNTLVIRGEGETE 152

[Close]

BLAST of Carg24846 vs. TAIR 10
Match: AT4G27670.1 (heat shock protein 21 )

HSP 1 Score: 55.1 bits (131), Expect = 5.7e-08
Identity = 32/76 (42.11%), Postives = 41/76 (53.95%), Query Frame = 0


Query: 73  DVFDPFSSHTQPKPGSESDGSVHGGSEQDQAGGDGTVREDDDALYLRMDMPGLSKEDVKV 132
           D  D     T P  G        GGS   +      ++E++  + +R DMPGLSKEDVK+
Sbjct: 100 DTMDRMFEDTMPVSGRN-----RGGSGVSEIRAPWDIKEEEHEIKMRFDMPGLSKEDVKI 159

Query: 133 SVEQNTLIIKGEAAKE 149
           SVE N L+IKGE  KE
Sbjct: 160 SVEDNVLVIKGEQKKE 170

[Close]

BLAST of Carg24846 vs. TAIR 10
Match: AT1G52560.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 5.9e-05
Identity = 21/42 (50.00%), Postives = 28/42 (66.67%), Query Frame = 0


Query: 107 GTVREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           G V+E DD   LR ++PGL+KEDVK++V    L IKG+   E
Sbjct: 127 GQVKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAE 168

[Close]

BLAST of Carg24846 vs. TAIR 10
Match: AT1G52560.2 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 5.9e-05
Identity = 21/42 (50.00%), Postives = 28/42 (66.67%), Query Frame = 0


Query: 107 GTVREDDDALYLRMDMPGLSKEDVKVSVEQNTLIIKGEAAKE 149
           G V+E DD   LR ++PGL+KEDVK++V    L IKG+   E
Sbjct: 120 GQVKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAE 161

The following BLAST results are available for this feature:

BLAST of Carg24846 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015728.1	5.3e-72	100.00	Heat shock 22 kDa protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosp...	[more]
KAG6577690.1	2.1e-49	77.50	Phosphatidylinositol/phosphatidylcholine transfer protein SFH11, partial [Cucurb...	[more]
XP_022923528.1	2.1e-49	77.50	small heat shock protein, chloroplastic-like isoform X2 [Cucurbita moschata] >XP...	[more]
XP_022965184.1	2.8e-49	77.50	small heat shock protein, chloroplastic-like isoform X2 [Cucurbita maxima]	[more]
XP_022923527.1	2.0e-47	76.40	small heat shock protein, chloroplastic-like isoform X1 [Cucurbita moschata] >XP...	[more]

BLAST of Carg24846 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39818	1.6e-18	45.34	Heat shock 22 kDa protein, mitochondrial OS=Glycine max OX=3847 GN=HSP23.9 PE=2 ...	[more]
P11890	6.1e-15	45.28	Small heat shock protein, chloroplastic OS=Oxybasis rubra OX=3560 GN=HSP23 PE=2 ...	[more]
P46254	1.5e-13	44.38	Heat shock 22 kDa protein, mitochondrial OS=Pisum sativum OX=3888 GN=HSP22 PE=2 ...	[more]
Q96331	1.5e-13	43.13	23.6 kDa heat shock protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HS...	[more]
Q9FGM9	3.7e-12	38.56	23.5 kDa heat shock protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HS...	[more]

BLAST of Carg24846 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E9W1	1.0e-49	77.50	small heat shock protein, chloroplastic-like isoform X2 OS=Cucurbita moschata OX...	[more]
A0A6J1HQA7	1.4e-49	77.50	small heat shock protein, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3...	[more]
A0A6J1E6C8	9.7e-48	76.40	small heat shock protein, chloroplastic-like isoform X1 OS=Cucurbita moschata OX...	[more]
A0A6J1HN48	1.3e-47	76.40	small heat shock protein, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3...	[more]
H6TB40	2.7e-45	72.50	HSP23.5 OS=Citrullus lanatus OX=3654 PE=2 SV=1	[more]

BLAST of Carg24846 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25200.1	1.1e-14	43.13	mitochondrion-localized small heat shock protein 23.6	[more]
AT5G51440.1	2.6e-13	38.56	HSP20-like chaperones superfamily protein	[more]
AT4G27670.1	5.7e-08	42.11	heat shock protein 21	[more]
AT1G52560.1	5.9e-05	50.00	HSP20-like chaperones superfamily protein	[more]
AT1G52560.2	5.9e-05	50.00	HSP20-like chaperones superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 58..149 e-value: 5.9E-11 score: 44.3
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 109..147
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 109..146 e-value: 2.6E-7 score: 30.7
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 98..149 score: 14.152247
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 105..120
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 81..97
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 47..62
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..120
None	No IPR available	PANTHER	PTHR46991:SF8	HEAT SHOCK 22 KDA PROTEIN, MITOCHONDRIAL-LIKE	coord: 1..80
None	No IPR available	PANTHER	PTHR46991:SF8	HEAT SHOCK 22 KDA PROTEIN, MITOCHONDRIAL-LIKE	coord: 108..147
None	No IPR available	CDD	cd06464	ACD_sHsps-like	coord: 109..144 e-value: 5.93265E-13 score: 58.7196
IPR044656	Heat shock protein HSP14.7/HSP23.5/HSP23.6-like	PANTHER	PTHR46991	23.5 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL	coord: 1..80
IPR044656	Heat shock protein HSP14.7/HSP23.5/HSP23.6-like	PANTHER	PTHR46991	23.5 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL	coord: 108..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24846-RA	Carg24846-RA	mRNA