Carg24775 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24775
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Chaperone protein dnaJ 20
Location	Carg_Chr14: 13566903 .. 13568330 (+)
RNA-Seq Expression	Carg24775
Synteny	Carg24775

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGACTCATTTGGACGTCCGGTATAAATAGCAGAAATACAGAGGACGGAAGATAGACGGAGAGAAACAGAGAAATGCAGTGCTCCGATTTTACCTTCTCAGGGAGCGATTCACGCTTCTACATCCCTTCGAGCCCTAGCATTACCGGCCGGCGACCAATTACTGGCCACCGATCTGGTATTTTCTTTCCCAACACTCTTCCGTGCAATTTGATGCGCTTGCCGTCTCTTTCGATCAGAGCCAAGGCATCGTTCAACGAAGGAATTGTCTCCTCCGAGGTTGCCGATGGCTCCTTCTACGATTTGCTTGGTGTATCTGAGTCCGTATCCTTGGCGGAAATCAAGCGCGCATACAAGCATCTAGCCCGGAAGTACCATCCGGACGTGTCGCCGATCGGCTGTGCGGAGGAGTATACGAAAAGGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCAGATCCGAGGAGGAGAGCTCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTTGGAGACTATACGACGCCGATCAGGTACTAGTTCACTCTACCGATTTTAATTTGTTTTCCGGTAATTTAGGGGTAATCGTGGTTGAATGTTTGCGGATTTTGAAATCTCCTGTGTTGATTTTGTGTTTGCCCTTGCTTTTGAGGTGATTTCGTGTTTAATTTCTCAGATGTTTTCCGAAGAGATTAAAATGAGTGAAGTATTTGATCTCAACGTATATTTGATTTTGGATTCTTTTGAGTGAGGAAATGAAACGTCGTTTTATTCTGCCTCCTGATTCTCTGTCTCTTTCCTTTTTTTTGTTTTTCTGATTGGTGGAGAGTGGTACTGTTTCTGTTGGAACAAGGTTCTTCCATTCGTTATCGTGATTTATTCTATAATTCAGAGATTGAATGGGATCGGTCAAGCAGATGATTTCTCGATAACTTACTCCAAATATGGAATTTCGTTACCTTCTCAACAACTTCTGTTTATATGCAACTTTTTTCACATAATTTTCTTTCAGTTTTCACTCTACTGTTTGTGTTCTAGCGAGTCTGTAGAAATCTTTTTCCTCCATATAGAATCTCCAAATTTGATTTGGATTCAGGAAGCTCCAGAGAAAAGTGGATGGAGGAGTTGTTGGCAATCTCAGATATCAGAATTGAAGAGAAGAAGCATGAACAAGGATTCGAGTCAAAATATGTCCTGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGATGGAATTTTAGAAACCACCATGTTTGACTTCAGTATATTCATACTGGAGCAACATAAGTTAATAAATAATACGCCCAATTTGATAACAATAATTTTGTTGCTAGTTTTGTTCCCCATTTCCTCTTACAATATGATTAAAACGTTTCTCAATCTAAAAACTAAACATCCTTAAAGATAG

mRNA sequence

TGACTCATTTGGACGTCCGGTATAAATAGCAGAAATACAGAGGACGGAAGATAGACGGAGAGAAACAGAGAAATGCAGTGCTCCGATTTTACCTTCTCAGGGAGCGATTCACGCTTCTACATCCCTTCGAGCCCTAGCATTACCGGCCGGCGACCAATTACTGGCCACCGATCTGGTATTTTCTTTCCCAACACTCTTCCGTGCAATTTGATGCGCTTGCCGTCTCTTTCGATCAGAGCCAAGGCATCGTTCAACGAAGGAATTGTCTCCTCCGAGGTTGCCGATGGCTCCTTCTACGATTTGCTTGGTGTATCTGAGTCCGTATCCTTGGCGGAAATCAAGCGCGCATACAAGCATCTAGCCCGGAAGTACCATCCGGACGTGTCGCCGATCGGCTGTGCGGAGGAGTATACGAAAAGGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCAGATCCGAGGAGGAGAGCTCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTTGGAGACTATACGACGCCGATCAGGAAGCTCCAGAGAAAAGTGGATGGAGGAGTTGTTGGCAATCTCAGATATCAGAATTGAAGAGAAGAAGCATGAACAAGGATTCGAGTCAAAATATGTCCTGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGATGGAATTTTAGAAACCACCATGTTTGACTTCAGTATATTCATACTGGAGCAACATAAGTTAATAAATAATACGCCCAATTTGATAACAATAATTTTGTTGCTAGTTTTGTTCCCCATTTCCTCTTACAATATGATTAAAACGTTTCTCAATCTAAAAACTAAACATCCTTAAAGATAG

Coding sequence (CDS)

ATGCAGTGCTCCGATTTTACCTTCTCAGGGAGCGATTCACGCTTCTACATCCCTTCGAGCCCTAGCATTACCGGCCGGCGACCAATTACTGGCCACCGATCTGGTATTTTCTTTCCCAACACTCTTCCGTGCAATTTGATGCGCTTGCCGTCTCTTTCGATCAGAGCCAAGGCATCGTTCAACGAAGGAATTGTCTCCTCCGAGGTTGCCGATGGCTCCTTCTACGATTTGCTTGGTGTATCTGAGTCCGTATCCTTGGCGGAAATCAAGCGCGCATACAAGCATCTAGCCCGGAAGTACCATCCGGACGTGTCGCCGATCGGCTGTGCGGAGGAGTATACGAAAAGGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCAGATCCGAGGAGGAGAGCTCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTTGGAGACTATACGACGCCGATCAGGAAGCTCCAGAGAAAAGTGGATGGAGGAGTTGTTGGCAATCTCAGATATCAGAATTGAAGAGAAGAAGCATGAACAAGGATTCGAGTCAAAATATGTCCTGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGA

Protein sequence

MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASFNEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRRSMNKDSSQNMSWGARMRRQMNEQV

Homology

[Close]

BLAST of Carg24775 vs. NCBI nr
Match: KAG7018873.1 (Chaperone protein dnaJ 20, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 412.9 bits (1060), Expect = 1.6e-111
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60
           MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF
Sbjct: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60

Query: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120
           NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV
Sbjct: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180
           QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180

Query: 181 SMNKDSSQNMSWGARMRRQMNEQV 205
           SMNKDSSQNMSWGARMRRQMNEQV
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQV 204

[Close]

BLAST of Carg24775 vs. NCBI nr
Match: XP_022924259.1 (chaperone protein dnaJ 20, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 409.1 bits (1050), Expect = 2.3e-110
Identity = 202/204 (99.02%), Postives = 202/204 (99.02%), Query Frame = 0


Query: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60
           MQC DFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF
Sbjct: 1   MQCPDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60

Query: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120
           NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIR 
Sbjct: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRF 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180
           QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180

Query: 181 SMNKDSSQNMSWGARMRRQMNEQV 205
           SMNKDSSQNMSWGARMRRQMNEQV
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQV 204

[Close]

BLAST of Carg24775 vs. NCBI nr
Match: KAG6582488.1 (Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 408.7 bits (1049), Expect = 3.0e-110
Identity = 202/203 (99.51%), Postives = 202/203 (99.51%), Query Frame = 0


Query: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60
           MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF
Sbjct: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60

Query: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120
           NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV
Sbjct: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180
           QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180

Query: 181 SMNKDSSQNMSWGARMRRQMNEQ 204
           SMN DSSQNMSWGARMRRQMNEQ
Sbjct: 181 SMNMDSSQNMSWGARMRRQMNEQ 203

[Close]

BLAST of Carg24775 vs. NCBI nr
Match: XP_023526392.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 403.3 bits (1035), Expect = 1.3e-108
Identity = 201/204 (98.53%), Postives = 201/204 (98.53%), Query Frame = 0


Query: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60
           MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF
Sbjct: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60

Query: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120
           NEG VSSEVADGSFYDLLGVSESVSLAEIKRAYK LARKYHPDVSPIG AEEYTKRFIRV
Sbjct: 61  NEGTVSSEVADGSFYDLLGVSESVSLAEIKRAYKQLARKYHPDVSPIGRAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180
           QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180

Query: 181 SMNKDSSQNMSWGARMRRQMNEQV 205
           SMNKDSSQNMSWGARMRRQMNEQV
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQV 204

[Close]

BLAST of Carg24775 vs. NCBI nr
Match: XP_022980417.1 (chaperone protein dnaJ 20, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 395.2 bits (1014), Expect = 3.4e-106
Identity = 197/204 (96.57%), Postives = 198/204 (97.06%), Query Frame = 0


Query: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60
           MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRS IFFPNTLPCNL RLPSLSIRAKASF
Sbjct: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSCIFFPNTLPCNLTRLPSLSIRAKASF 60

Query: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120
           NEGIVSSEV+DGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIG AEEYTKRFIRV
Sbjct: 61  NEGIVSSEVSDGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGQAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180
            EAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQ QISEL RR
Sbjct: 121 HEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQFQISELNRR 180

Query: 181 SMNKDSSQNMSWGARMRRQMNEQV 205
           SMNKDSSQNMSWGARMRRQMNEQV
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQV 204

[Close]

BLAST of Carg24775 vs. ExPASy Swiss-Prot
Match: Q9SDN0 (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 2.6e-40
Identity = 95/170 (55.88%), Postives = 117/170 (68.82%), Query Frame = 0


Query: 39  PNTLPCNL-----MRLPSLSIRAKASFNEGIVSSEVADGSFYDLLGVSESVSLAEIKRAY 98
           P ++P  +      R  S  I+++ + ++ +  SE  D SFYDLLGV+ESV+L EIK+AY
Sbjct: 29  PTSIPTTISYPTRTRFSSTRIQSRLTHDDPVKQSE--DLSFYDLLGVTESVTLPEIKQAY 88

Query: 99  KHLARKYHPDVSPIGCAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSAWRL 158
           K LARKYHPDVSP    EEYT RFIRVQEAYETLSDPRRR LYDRD+  G   +FS  R 
Sbjct: 89  KQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQ 148

Query: 159 YDADQE-APEKSGWRSCWQSQISELKRRSMNKDSSQNMSWGARMRRQMNE 203
              DQE   EKS W++ WQ+Q+S L+RRS  KD++  MSW ARMRRQ  E
Sbjct: 149 NRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKDNN-TMSWAARMRRQQQE 195

[Close]

BLAST of Carg24775 vs. ExPASy Swiss-Prot
Match: A5VJE8 (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) OX=557436 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.3e-12
Identity = 40/79 (50.63%), Postives = 54/79 (68.35%), Query Frame = 0


Query: 69  VADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRVQEAYETLS 128
           +A+  +YD+LGVS+  S  +IKRAY+ LA KYHPDV+    AEE   +F ++ EAYETLS
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQVA 148
           D ++RA YD+    G Q A
Sbjct: 61  DSQKRAQYDQFGSAGPQGA 76

[Close]

BLAST of Carg24775 vs. ExPASy Swiss-Prot
Match: B2G6W4 (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) OX=557433 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.3e-12
Identity = 40/79 (50.63%), Postives = 54/79 (68.35%), Query Frame = 0


Query: 69  VADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRVQEAYETLS 128
           +A+  +YD+LGVS+  S  +IKRAY+ LA KYHPDV+    AEE   +F ++ EAYETLS
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQVA 148
           D ++RA YD+    G Q A
Sbjct: 61  DSQKRAQYDQFGSAGPQGA 76

[Close]

BLAST of Carg24775 vs. ExPASy Swiss-Prot
Match: B2GBQ6 (Chaperone protein DnaJ OS=Lactobacillus fermentum (strain NBRC 3956 / LMG 18251) OX=334390 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.8e-12
Identity = 40/77 (51.95%), Postives = 52/77 (67.53%), Query Frame = 0


Query: 69  VADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRVQEAYETLS 128
           +A+   YD+LGV +  S AEIKRAY+ LA KYHPDV+    AE   K+F ++ EAYETLS
Sbjct: 1   MAEEDLYDVLGVKKDASEAEIKRAYRKLAAKYHPDVNHEPGAE---KKFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQ 146
           D ++RA YD+    G Q
Sbjct: 61  DDQKRAQYDQFGTTGSQ 74

[Close]

BLAST of Carg24775 vs. ExPASy Swiss-Prot
Match: Q3K3T1 (Chaperone protein DnaJ OS=Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700) OX=205921 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.8e-12
Identity = 37/78 (47.44%), Postives = 51/78 (65.38%), Query Frame = 0


Query: 74  FYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRVQEAYETLSDPRRR 133
           FYD LGVS+  S  EIK+AY+ +++KYHPD++    AEE   ++  VQEAYETLSD ++R
Sbjct: 6   FYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKEAGAEE---KYKEVQEAYETLSDTQKR 65

Query: 134 ALYDRDMVGGLQVAFSAW 152
           A YD+    G    F  +
Sbjct: 66  AAYDQYGAAGANGGFGGF 80

[Close]

BLAST of Carg24775 vs. ExPASy TrEMBL
Match: A0A6J1E8M4 (chaperone protein dnaJ 20, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431786 PE=4 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.1e-110
Identity = 202/204 (99.02%), Postives = 202/204 (99.02%), Query Frame = 0


Query: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60
           MQC DFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF
Sbjct: 1   MQCPDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60

Query: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120
           NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIR 
Sbjct: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRF 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180
           QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180

Query: 181 SMNKDSSQNMSWGARMRRQMNEQV 205
           SMNKDSSQNMSWGARMRRQMNEQV
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQV 204

[Close]

BLAST of Carg24775 vs. ExPASy TrEMBL
Match: A0A6J1IRB6 (chaperone protein dnaJ 20, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479792 PE=4 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.7e-106
Identity = 197/204 (96.57%), Postives = 198/204 (97.06%), Query Frame = 0


Query: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60
           MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRS IFFPNTLPCNL RLPSLSIRAKASF
Sbjct: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSCIFFPNTLPCNLTRLPSLSIRAKASF 60

Query: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120
           NEGIVSSEV+DGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIG AEEYTKRFIRV
Sbjct: 61  NEGIVSSEVSDGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGQAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180
            EAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQ QISEL RR
Sbjct: 121 HEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQFQISELNRR 180

Query: 181 SMNKDSSQNMSWGARMRRQMNEQV 205
           SMNKDSSQNMSWGARMRRQMNEQV
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQV 204

[Close]

BLAST of Carg24775 vs. ExPASy TrEMBL
Match: A0A1S3AWS2 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483465 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.7e-88
Identity = 167/204 (81.86%), Postives = 183/204 (89.71%), Query Frame = 0


Query: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60
           MQCSDFTFSGSDSRFYIPS+P+I+ RRPI+G+RS +FFP+T P N  RLPSLSIRAKASF
Sbjct: 27  MQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTRLPSLSIRAKASF 86

Query: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120
           NEG VSSEVA+GSFYDLLG+S+S SLAEIKRAYK LARKYHPDVSP G AEEYTK FIRV
Sbjct: 87  NEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPGGAEEYTKMFIRV 146

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180
           QEAYETLSDPRRRALYDRDM+GGLQVAFSA R YDA +E  EKSGWR+ W++QISELKRR
Sbjct: 147 QEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRNSWEAQISELKRR 206

Query: 181 SMNKDSSQNMSWGARMRRQMNEQV 205
           SM KD  QNMSWGARMRRQMNEQ+
Sbjct: 207 SMEKDLRQNMSWGARMRRQMNEQI 230

[Close]

BLAST of Carg24775 vs. ExPASy TrEMBL
Match: A0A6J1IB32 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111473471 PE=4 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 2.9e-87
Identity = 165/204 (80.88%), Postives = 178/204 (87.25%), Query Frame = 0


Query: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60
           MQCSDFT+SG D R  IPSSPSITGRR I+G +S +FFPNT PCN  RLPSLSIRAKASF
Sbjct: 1   MQCSDFTYSGGDYRILIPSSPSITGRRQISGQKSRVFFPNTPPCNSTRLPSLSIRAKASF 60

Query: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120
           N GI S E ADGSFYDLLG+SES SLAEIKRAYK LARKYHPDVSP G AEEYTKRFIRV
Sbjct: 61  NGGIASFEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180
           QEAYETLSDPRRRALYDRDMVGGLQVAFSA R YD D+  P+KS W+SCW++QISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKSCWKAQISELKRR 180

Query: 181 SMNKDSSQNMSWGARMRRQMNEQV 205
           SM+KDS  N+SWGARMR+QMNEQV
Sbjct: 181 SMDKDSKHNLSWGARMRQQMNEQV 204

[Close]

BLAST of Carg24775 vs. ExPASy TrEMBL
Match: A0A6J1GCT3 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452997 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 6.5e-87
Identity = 165/204 (80.88%), Postives = 178/204 (87.25%), Query Frame = 0


Query: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60
           MQCSDFT+S  D R  IPSSPSITGRR I+G RS +FFPNT PCN  RLPSLSIRAKASF
Sbjct: 35  MQCSDFTYSVGDYRILIPSSPSITGRRQISGQRSRVFFPNTPPCNSTRLPSLSIRAKASF 94

Query: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120
           N GI SSE ADGSFYDLLG+SES SLAEIKRAYK LARKYHPDVSP G AEEYTKRFIRV
Sbjct: 95  NGGIASSEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 154

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180
           QEAYETLSDPRRRALYDRDMVGGLQVAFSA R YD D+  P+KS W+SCW++QISELK+R
Sbjct: 155 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKSCWKAQISELKKR 214

Query: 181 SMNKDSSQNMSWGARMRRQMNEQV 205
           SM+KDS  N+SWGARMR+QMNEQV
Sbjct: 215 SMDKDSKHNLSWGARMRQQMNEQV 238

[Close]

BLAST of Carg24775 vs. TAIR 10
Match: AT4G13830.2 (DNAJ-like 20 )

HSP 1 Score: 166.8 bits (421), Expect = 1.8e-41
Identity = 95/170 (55.88%), Postives = 117/170 (68.82%), Query Frame = 0


Query: 39  PNTLPCNL-----MRLPSLSIRAKASFNEGIVSSEVADGSFYDLLGVSESVSLAEIKRAY 98
           P ++P  +      R  S  I+++ + ++ +  SE  D SFYDLLGV+ESV+L EIK+AY
Sbjct: 29  PTSIPTTISYPTRTRFSSTRIQSRLTHDDPVKQSE--DLSFYDLLGVTESVTLPEIKQAY 88

Query: 99  KHLARKYHPDVSPIGCAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSAWRL 158
           K LARKYHPDVSP    EEYT RFIRVQEAYETLSDPRRR LYDRD+  G   +FS  R 
Sbjct: 89  KQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQ 148

Query: 159 YDADQE-APEKSGWRSCWQSQISELKRRSMNKDSSQNMSWGARMRRQMNE 203
              DQE   EKS W++ WQ+Q+S L+RRS  KD++  MSW ARMRRQ  E
Sbjct: 149 NRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKDNN-TMSWAARMRRQQQE 195

[Close]

BLAST of Carg24775 vs. TAIR 10
Match: AT4G13830.1 (DNAJ-like 20 )

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-29
Identity = 70/125 (56.00%), Postives = 85/125 (68.00%), Query Frame = 0


Query: 39  PNTLPCNL-----MRLPSLSIRAKASFNEGIVSSEVADGSFYDLLGVSESVSLAEIKRAY 98
           P ++P  +      R  S  I+++ + ++ +  SE  D SFYDLLGV+ESV+L EIK+AY
Sbjct: 29  PTSIPTTISYPTRTRFSSTRIQSRLTHDDPVKQSE--DLSFYDLLGVTESVTLPEIKQAY 88

Query: 99  KHLARKYHPDVSPIGCAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSAWRL 158
           K LARKYHPDVSP    EEYT RFIRVQEAYETLSDPRRR LYDRD+  G   +FS  R 
Sbjct: 89  KQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQ 148

[Close]

BLAST of Carg24775 vs. TAIR 10
Match: AT4G39960.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.1e-13
Identity = 45/115 (39.13%), Postives = 62/115 (53.91%), Query Frame = 0


Query: 33  RSGIFFPNTLPCNLMRLPSLSIRAKASFNEGIVSSEVADGSFYDLLGVSESVSLAEIKRA 92
           RS +F   +LP     L    +        G   +  AD  FY +LGVS++ + AEIK A
Sbjct: 49  RSSMFAQGSLPF----LSLTGVSPNTHSRRGARFTVRADTDFYSVLGVSKNATKAEIKSA 108

Query: 93  YKHLARKYHPDVSPIGCAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVA 148
           Y+ LAR YHPDV+    AE+   +F  +  AYE LSD  +R+LYDR    G++ A
Sbjct: 109 YRKLARSYHPDVNKDAGAED---KFKEISNAYEILSDDEKRSLYDRYGEAGVKGA 156

[Close]

BLAST of Carg24775 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 72.8 bits (177), Expect = 3.6e-13
Identity = 38/78 (48.72%), Postives = 51/78 (65.38%), Query Frame = 0


Query: 70  ADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRVQEAYETLSD 129
           AD  +Y +LGVS++ + AEIK AY+ LAR YHPDV+    AEE   +F  +  AYE LSD
Sbjct: 83  ADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEE---KFKEISNAYEVLSD 142

Query: 130 PRRRALYDRDMVGGLQVA 148
             +++LYDR    GL+ A
Sbjct: 143 DEKKSLYDRYGEAGLKGA 157

[Close]

BLAST of Carg24775 vs. TAIR 10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.6e-11
Identity = 34/67 (50.75%), Postives = 46/67 (68.66%), Query Frame = 0


Query: 72  GSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRVQEAYETLSDPR 131
           G +Y  LGVS+S +  EIK AY+ LAR+YHPDV+    A   T++F  +  AYE LSD +
Sbjct: 74  GDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEPGA---TEKFKEISAAYEVLSDEQ 133

Query: 132 RRALYDR 139
           +RALYD+
Sbjct: 134 KRALYDQ 137

The following BLAST results are available for this feature:

BLAST of Carg24775 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018873.1	1.6e-111	100.00	Chaperone protein dnaJ 20, chloroplastic [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_022924259.1	2.3e-110	99.02	chaperone protein dnaJ 20, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
KAG6582488.1	3.0e-110	99.51	Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
XP_023526392.1	1.3e-108	98.53	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022980417.1	3.4e-106	96.57	chaperone protein dnaJ 20, chloroplastic-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg24775 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SDN0	2.6e-40	55.88	Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ2...	[more]
A5VJE8	1.3e-12	50.63	Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) OX=557436 GN=...	[more]
B2G6W4	1.3e-12	50.63	Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) OX=557433 GN=d...	[more]
B2GBQ6	1.8e-12	51.95	Chaperone protein DnaJ OS=Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)...	[more]
Q3K3T1	1.8e-12	47.44	Chaperone protein DnaJ OS=Streptococcus agalactiae serotype Ia (strain ATCC 2759...	[more]

BLAST of Carg24775 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E8M4	1.1e-110	99.02	chaperone protein dnaJ 20, chloroplastic-like isoform X1 OS=Cucurbita moschata O...	[more]
A0A6J1IRB6	1.7e-106	96.57	chaperone protein dnaJ 20, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=...	[more]
A0A1S3AWS2	2.7e-88	81.86	chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1IB32	2.9e-87	80.88	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1GCT3	6.5e-87	80.88	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of Carg24775 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13830.2	1.8e-41	55.88	DNAJ-like 20	[more]
AT4G13830.1	1.2e-29	56.00	DNAJ-like 20	[more]
AT4G39960.1	2.1e-13	39.13	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT2G22360.1	3.6e-13	48.72	DNAJ heat shock family protein	[more]
AT1G80030.1	2.6e-11	50.75	Molecular chaperone Hsp40/DnaJ family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 75..93 score: 48.82 coord: 112..132 score: 39.13 coord: 93..108 score: 48.44
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 72..132 e-value: 3.2E-19 score: 79.9
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 74..137 e-value: 1.6E-20 score: 73.0
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 73..140 score: 19.794384
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 74..129 e-value: 8.82257E-21 score: 79.5117
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 71..196 e-value: 4.8E-23 score: 83.8
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 73..146
None	No IPR available	PANTHER	PTHR45090	CHAPERONE PROTEIN DNAJ 20 CHLOROPLASTIC	coord: 1..203
None	No IPR available	PANTHER	PTHR45090:SF4	CHAPERONE PROTEIN DNAJ 20, CHLOROPLASTIC	coord: 1..203
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 117..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24775-RA	Carg24775-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061077	chaperone-mediated protein folding
cellular_component	GO:0009507	chloroplast