Carg24731 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg24731 vs. NCBI nr
Match: KAG7024996.1 (hypothetical protein SDJN02_13816, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 470.3 bits (1209), Expect = 9.6e-129
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0

Query: 1   MGESSRKWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60
           MGESSRKWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD
Sbjct: 1   MGESSRKWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60

Query: 61  IKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLH 120
           IKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLH
Sbjct: 61  IKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLH 120

Query: 121 QNLVRRFLRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPG 180
           QNLVRRFLRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPG
Sbjct: 121 QNLVRRFLRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPG 180

Query: 181 YVNKKGSGPNSNLTFPVGLCSTFKFVKTLSKKMREKRLAKAAEFSQLMFHLSV 234
           YVNKKGSGPNSNLTFPVGLCSTFKFVKTLSKKMREKRLAKAAEFSQLMFHLSV
Sbjct: 181 YVNKKGSGPNSNLTFPVGLCSTFKFVKTLSKKMREKRLAKAAEFSQLMFHLSV 233

BLAST of Carg24731 vs. NCBI nr
Match: XP_022936898.1 (uncharacterized protein At4g26485 isoform X1 [Cucurbita moschata])

HSP 1 Score: 411.8 bits (1057), Expect = 4.0e-111
Identity = 206/227 (90.75%), Postives = 213/227 (93.83%), Query Frame = 0

Query: 1   MGESSRKWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60
           MG+SSRKWITHY SSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD
Sbjct: 1   MGKSSRKWITHYSSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60

Query: 61  IKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLH 120
           IKTTLEELKELGCSVMHGVDV TMSQHPLLCHTLFDRIVFNFPHAGFQYS  ++   KLH
Sbjct: 61  IKTTLEELKELGCSVMHGVDVMTMSQHPLLCHTLFDRIVFNFPHAGFQYSRDKSS--KLH 120

Query: 121 QNLVRRFLRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPG 180
           QNLVRRFLRNAKEL  ENG+IHITHKIS+PYSEWEIEEVAEEEDLFLRE VEFN GDYPG
Sbjct: 121 QNLVRRFLRNAKELFVENGEIHITHKISYPYSEWEIEEVAEEEDLFLREEVEFNEGDYPG 180

Query: 181 YVNKKGSGPNSNLTFPVGLCSTFKFVKTLSKKMREKRLAKAAEFSQL 228
           YVNKKGSGPNSN TF VGLCSTFKFVKTLSKK R+KRLAKAAEF+QL
Sbjct: 181 YVNKKGSGPNSNRTFAVGLCSTFKFVKTLSKKTRKKRLAKAAEFAQL 225

BLAST of Carg24731 vs. NCBI nr
Match: XP_022976038.1 (uncharacterized protein At4g26485 isoform X1 [Cucurbita maxima])

HSP 1 Score: 385.2 bits (988), Expect = 4.0e-103
Identity = 196/233 (84.12%), Postives = 209/233 (89.70%), Query Frame = 0

Query: 1   MGESSRKWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60
           MG S RKWITHY SSHDILLVGEGNFSFSACLASAFGTATN+VATSLDSEETLLRKYGSD
Sbjct: 1   MGMSPRKWITHYSSSHDILLVGEGNFSFSACLASAFGTATNMVATSLDSEETLLRKYGSD 60

Query: 61  IKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYS-EHETRQIKL 120
           IKTTLEELKELGCSV+HGVDV TMSQH LLCHT FDRIVFNFPHAGFQYS EHET QIKL
Sbjct: 61  IKTTLEELKELGCSVIHGVDVATMSQHLLLCHTWFDRIVFNFPHAGFQYSREHETGQIKL 120

Query: 121 HQNLVRRFLRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYP 180
           HQNLVR FLRNAKELLAENG+IHITHKIS+PYSEW+IE++AEEE LFLRE VEF+  DYP
Sbjct: 121 HQNLVRSFLRNAKELLAENGEIHITHKISYPYSEWKIEKIAEEEGLFLREEVEFDKWDYP 180

Query: 181 GYVNKKGSGPNSNLTFPVGLCSTFKFVKTLSKKMREKRLAKAAEFSQLMFHLS 233
            Y NKKGSGPNSN TFPVGLC TFKFVKTLS+  RE+RLA  AEF++L   +S
Sbjct: 181 CYDNKKGSGPNSNRTFPVGLCCTFKFVKTLSR--RERRLATVAEFAKLNLSMS 231

BLAST of Carg24731 vs. NCBI nr
Match: KAG6592122.1 (hypothetical protein SDJN03_14468, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 349.0 bits (894), Expect = 3.2e-92
Identity = 174/186 (93.55%), Postives = 177/186 (95.16%), Query Frame = 0

Query: 1   MGESSRKWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60
           MG+SSRKWITHY SSHDILLVGEGNFSFS CLAS FGTATNVVATSLDSEETLLRKYGSD
Sbjct: 1   MGKSSRKWITHYSSSHDILLVGEGNFSFSVCLASFFGTATNVVATSLDSEETLLRKYGSD 60

Query: 61  IKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYS-EHETRQIKL 120
           IKTTLEELKELGCSVMHGVDV TMSQHPLLCHTLFDRIVFNFPHAGFQYS EHETRQIKL
Sbjct: 61  IKTTLEELKELGCSVMHGVDVMTMSQHPLLCHTLFDRIVFNFPHAGFQYSREHETRQIKL 120

Query: 121 HQNLVRRFLRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYP 180
           HQNLV RFLRNAKELLAENG+IHITHKIS+PYSEWEIE VAEEEDLFLRE VEFN GDYP
Sbjct: 121 HQNLVIRFLRNAKELLAENGEIHITHKISYPYSEWEIELVAEEEDLFLREEVEFNEGDYP 180

Query: 181 GYVNKK 186
           GYVNKK
Sbjct: 181 GYVNKK 186

BLAST of Carg24731 vs. NCBI nr
Match: XP_022139694.1 (uncharacterized protein At4g26485-like [Momordica charantia])

HSP 1 Score: 303.1 bits (775), Expect = 2.0e-78
Identity = 152/218 (69.72%), Postives = 175/218 (80.28%), Query Frame = 0

Query: 11  HYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIKTTLEELKE 70
           HY SSH ILLVGEGNFSFS CLA+A G+  N+VATSLDS++TLLRKYG +I+TTLEEL+E
Sbjct: 2   HYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELEE 61

Query: 71  LGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLHQNLVRRFLRN 130
            GC VMHGV+V TMSQHPLL + LFDRIVFNFPHAGF YSE E +QIKLHQNLVR F+RN
Sbjct: 62  EGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVRN 121

Query: 131 AKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPGYVNKKGSGPN 190
           A E+++ENG+IHITHK S P+SEWEI E+AEEE LFL+E  EF+  DYPGY+NKKG GP 
Sbjct: 122 AVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGPY 181

Query: 191 SNLTFPVGLCSTFKFVKTLSKKMREK-RLAKAAEFSQL 228
            N TFPVG C TFKF K L K    K   + AAEF+ L
Sbjct: 182 CNRTFPVGCCCTFKFAKALPKDKHNKVSNSLAAEFAHL 219

BLAST of Carg24731 vs. ExPASy Swiss-Prot
Match: P0C8L4 (Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.8e-53
Identity = 111/209 (53.11%), Postives = 143/209 (68.42%), Query Frame = 0

Query: 3   ESSRKWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIK 62
           E S KWI HY S+H ILLVGEGNFSFS CLASAFG+A N+ ATSLDSE+ L  KY  D  
Sbjct: 2   EISEKWIRHYSSTHKILLVGEGNFSFSLCLASAFGSAMNITATSLDSEDELSIKY-MDAV 61

Query: 63  TTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQY--SEHETRQIKLH 122
             +  LK  GC + H VDV TMS    L    +DRIVFNFPHAG ++   E  +R I+ H
Sbjct: 62  DNINILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFNFPHAGSRFFGRELSSRAIESH 121

Query: 123 QNLVRRFLRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPG 182
           + LVR FL NAKE+L E+G+IHITHK ++P+S+W I+++ + E L L +  +F +  YPG
Sbjct: 122 KELVRGFLENAKEMLEEDGEIHITHKTTYPFSDWGIKKLGKGEGLKLLKKSKFELSHYPG 181

Query: 183 YVNKKGS-GPNSNLTFPVGLCSTFKFVKT 209
           Y+ K+GS G  S+  FPVG CST+ F ++
Sbjct: 182 YITKRGSGGRRSDDYFPVGECSTYMFTQS 209

BLAST of Carg24731 vs. ExPASy Swiss-Prot
Match: F4I1X0 (Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX=3702 GN=HIPP41 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 2.7e-49
Identity = 96/198 (48.48%), Postives = 132/198 (66.67%), Query Frame = 0

Query: 8   WITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIKTTLEE 67
           W+ HY S+H ILLVGEG+FSFS  LA+ FG+A+N+ A+SLDS + ++RKY    ++ L+ 
Sbjct: 20  WVKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKY-KKARSNLKT 79

Query: 68  LKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLHQNLVRRF 127
           LK LG  ++HGVD TT+  HP L +  FDR++FNFPHAGF   E ++  I+ H+ LV  F
Sbjct: 80  LKRLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKHRELVFGF 139

Query: 128 LRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPGYVNKKGS 187
              A  LL  NG++H++HK   P+SEW +EE+A    L L + V F   +YPGY NK+G 
Sbjct: 140 FNGASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYPGYENKRGD 199

Query: 188 GPNSNLTFPVGLCSTFKF 206
           G   +  F +G CSTFKF
Sbjct: 200 GRRCDQPFLLGECSTFKF 216

BLAST of Carg24731 vs. ExPASy Swiss-Prot
Match: O94480 (25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bmt5 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.0e-16
Identity = 58/185 (31.35%), Postives = 100/185 (54.05%), Query Frame = 0

Query: 5   SRKWITHYGSSHDILLVGEGNFSFS-ACLASAFGTATNVVATSLDSEETLLRKYGSDIKT 64
           + +++  +  ++  LL+GEGNFSF+ + L     +   V+ATS DS+E L +KY  D   
Sbjct: 47  TERYVLPFEKNNRFLLLGEGNFSFAFSLLLHHVSSEGFVLATSYDSKEDLKQKY-PDAAE 106

Query: 65  TLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLHQNL 124
            + +++  G  VMH +D T +  H  L    FD I +NFPH+G    + + R I  +Q +
Sbjct: 107 YISKIEINGGKVMHEIDATKLHLHKKLKTQKFDTIFWNFPHSGKGIKDQD-RNILDNQKM 166

Query: 125 VRRFLRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPGYVN 184
           +  F + +K LL+E G I IT   + PY+ W ++ +A++         +F+   YP Y +
Sbjct: 167 LLAFFKASKFLLSEKGVIVITLAETKPYTLWNLKGLAKDAGYTSLMTEKFDSSFYPEYSH 226

Query: 185 KKGSG 189
           ++  G
Sbjct: 227 RRTIG 229

BLAST of Carg24731 vs. ExPASy Swiss-Prot
Match: Q3UY23 (Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus musculus OX=10090 GN=Fdxacb1 PE=2 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-11
Identity = 53/176 (30.11%), Postives = 82/176 (46.59%), Query Frame = 0

Query: 18  ILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIK-TTLEELKELGCSVM 77
           +LLVGEGNFSF+A L      + +V AT       L    G  +    L+ L+E G  V 
Sbjct: 6   LLLVGEGNFSFAASLIDGLDPSVSVTATGFQHRAAL---EGDPVALENLKRLRERGVEVR 65

Query: 78  HGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLHQNLVRRFLRNAKELLA 137
            GVD T +S         FDRI FNFPH G +    + R+      L+ +F ++  ++LA
Sbjct: 66  FGVDCTQLSHALPADDRDFDRIYFNFPHCGRKAGVAKNRE------LLAKFFQSCADVLA 125

Query: 138 ENGDIHIT-------HKISFP----YSEWEIEEVAEEEDLFLRELVEFNIGDYPGY 182
           + G++H+T            P    ++ W++  +A      L ++  F+    PGY
Sbjct: 126 KAGEVHVTLCRGQGGTPADKPQREWHNSWQVVAMAALGGFILSDVCPFSCEAVPGY 172

BLAST of Carg24731 vs. ExPASy Swiss-Prot
Match: Q9BRP7 (Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX=9606 GN=FDXACB1 PE=1 SV=3)

HSP 1 Score: 67.8 bits (164), Expect = 1.9e-10
Identity = 52/175 (29.71%), Postives = 82/175 (46.86%), Query Frame = 0

Query: 18  ILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIKTTLEELKELGCSVMH 77
           +LLVGEGNFSF+A L+     +T + AT L     L R         L+ L+E G  V  
Sbjct: 6   LLLVGEGNFSFAAALSETLDQSTQLTATCLQRPAELAR--DPLAWENLQCLRERGIDVRF 65

Query: 78  GVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLHQNLVRRFLRNAKELLAE 137
           GVD T ++    L    FD+I F FPH G +    + R+      L+ +F ++  ++LAE
Sbjct: 66  GVDCTQLADVFELHEREFDQIYFIFPHCGRKAGVAKNRE------LLAKFFQSCADVLAE 125

Query: 138 NGDIHIT-------HKISFP----YSEWEIEEVAEEEDLFLRELVEFNIGDYPGY 182
            G++H+             P    ++ W++  +A    L L ++  F+     GY
Sbjct: 126 EGEVHVALCRGQGGTPADKPQREWHNSWQVVAMAALGGLILSDVYPFSCKAVAGY 172

BLAST of Carg24731 vs. ExPASy TrEMBL
Match: A0A6J1FEI5 (uncharacterized protein At4g26485 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443349 PE=4 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 2.0e-111
Identity = 206/227 (90.75%), Postives = 213/227 (93.83%), Query Frame = 0

Query: 1   MGESSRKWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60
           MG+SSRKWITHY SSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD
Sbjct: 1   MGKSSRKWITHYSSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60

Query: 61  IKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLH 120
           IKTTLEELKELGCSVMHGVDV TMSQHPLLCHTLFDRIVFNFPHAGFQYS  ++   KLH
Sbjct: 61  IKTTLEELKELGCSVMHGVDVMTMSQHPLLCHTLFDRIVFNFPHAGFQYSRDKSS--KLH 120

Query: 121 QNLVRRFLRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPG 180
           QNLVRRFLRNAKEL  ENG+IHITHKIS+PYSEWEIEEVAEEEDLFLRE VEFN GDYPG
Sbjct: 121 QNLVRRFLRNAKELFVENGEIHITHKISYPYSEWEIEEVAEEEDLFLREEVEFNEGDYPG 180

Query: 181 YVNKKGSGPNSNLTFPVGLCSTFKFVKTLSKKMREKRLAKAAEFSQL 228
           YVNKKGSGPNSN TF VGLCSTFKFVKTLSKK R+KRLAKAAEF+QL
Sbjct: 181 YVNKKGSGPNSNRTFAVGLCSTFKFVKTLSKKTRKKRLAKAAEFAQL 225

BLAST of Carg24731 vs. ExPASy TrEMBL
Match: A0A6J1IKX9 (uncharacterized protein At4g26485 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476560 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 2.0e-103
Identity = 196/233 (84.12%), Postives = 209/233 (89.70%), Query Frame = 0

Query: 1   MGESSRKWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60
           MG S RKWITHY SSHDILLVGEGNFSFSACLASAFGTATN+VATSLDSEETLLRKYGSD
Sbjct: 1   MGMSPRKWITHYSSSHDILLVGEGNFSFSACLASAFGTATNMVATSLDSEETLLRKYGSD 60

Query: 61  IKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYS-EHETRQIKL 120
           IKTTLEELKELGCSV+HGVDV TMSQH LLCHT FDRIVFNFPHAGFQYS EHET QIKL
Sbjct: 61  IKTTLEELKELGCSVIHGVDVATMSQHLLLCHTWFDRIVFNFPHAGFQYSREHETGQIKL 120

Query: 121 HQNLVRRFLRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYP 180
           HQNLVR FLRNAKELLAENG+IHITHKIS+PYSEW+IE++AEEE LFLRE VEF+  DYP
Sbjct: 121 HQNLVRSFLRNAKELLAENGEIHITHKISYPYSEWKIEKIAEEEGLFLREEVEFDKWDYP 180

Query: 181 GYVNKKGSGPNSNLTFPVGLCSTFKFVKTLSKKMREKRLAKAAEFSQLMFHLS 233
            Y NKKGSGPNSN TFPVGLC TFKFVKTLS+  RE+RLA  AEF++L   +S
Sbjct: 181 CYDNKKGSGPNSNRTFPVGLCCTFKFVKTLSR--RERRLATVAEFAKLNLSMS 231

BLAST of Carg24731 vs. ExPASy TrEMBL
Match: A0A6J1CDR1 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111010540 PE=4 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 9.8e-79
Identity = 152/218 (69.72%), Postives = 175/218 (80.28%), Query Frame = 0

Query: 11  HYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIKTTLEELKE 70
           HY SSH ILLVGEGNFSFS CLA+A G+  N+VATSLDS++TLLRKYG +I+TTLEEL+E
Sbjct: 2   HYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELEE 61

Query: 71  LGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLHQNLVRRFLRN 130
            GC VMHGV+V TMSQHPLL + LFDRIVFNFPHAGF YSE E +QIKLHQNLVR F+RN
Sbjct: 62  EGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVRN 121

Query: 131 AKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPGYVNKKGSGPN 190
           A E+++ENG+IHITHK S P+SEWEI E+AEEE LFL+E  EF+  DYPGY+NKKG GP 
Sbjct: 122 AVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGPY 181

Query: 191 SNLTFPVGLCSTFKFVKTLSKKMREK-RLAKAAEFSQL 228
            N TFPVG C TFKF K L K    K   + AAEF+ L
Sbjct: 182 CNRTFPVGCCCTFKFAKALPKDKHNKVSNSLAAEFAHL 219

BLAST of Carg24731 vs. ExPASy TrEMBL
Match: A0A6J1IME0 (uncharacterized protein At4g26485 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476560 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 2.0e-68
Identity = 135/147 (91.84%), Postives = 138/147 (93.88%), Query Frame = 0

Query: 1   MGESSRKWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60
           MG S RKWITHY SSHDILLVGEGNFSFSACLASAFGTATN+VATSLDSEETLLRKYGSD
Sbjct: 1   MGMSPRKWITHYSSSHDILLVGEGNFSFSACLASAFGTATNMVATSLDSEETLLRKYGSD 60

Query: 61  IKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYS-EHETRQIKL 120
           IKTTLEELKELGCSV+HGVDV TMSQH LLCHT FDRIVFNFPHAGFQYS EHET QIKL
Sbjct: 61  IKTTLEELKELGCSVIHGVDVATMSQHLLLCHTWFDRIVFNFPHAGFQYSREHETGQIKL 120

Query: 121 HQNLVRRFLRNAKELLAENGDIHITHK 147
           HQNLVR FLRNAKELLAENG+IHITHK
Sbjct: 121 HQNLVRSFLRNAKELLAENGEIHITHK 147

BLAST of Carg24731 vs. ExPASy TrEMBL
Match: A0A6J1F9M1 (uncharacterized protein At4g26485 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443349 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 4.6e-68
Identity = 133/146 (91.10%), Postives = 137/146 (93.84%), Query Frame = 0

Query: 1   MGESSRKWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60
           MG+SSRKWITHY SSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD
Sbjct: 1   MGKSSRKWITHYSSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSD 60

Query: 61  IKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLH 120
           IKTTLEELKELGCSVMHGVDV TMSQHPLLCHTLFDRIVFNFPHAGFQYS  ++   KLH
Sbjct: 61  IKTTLEELKELGCSVMHGVDVMTMSQHPLLCHTLFDRIVFNFPHAGFQYSRDKSS--KLH 120

Query: 121 QNLVRRFLRNAKELLAENGDIHITHK 147
           QNLVRRFLRNAKEL  ENG+IHITHK
Sbjct: 121 QNLVRRFLRNAKELFVENGEIHITHK 144

BLAST of Carg24731 vs. TAIR 10
Match: AT1G55790.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 196.8 bits (499), Expect = 1.9e-50
Identity = 96/198 (48.48%), Postives = 132/198 (66.67%), Query Frame = 0

Query: 8   WITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIKTTLEE 67
           W+ HY S+H ILLVGEG+FSFS  LA+ FG+A+N+ A+SLDS + ++RKY    ++ L+ 
Sbjct: 20  WVKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKY-KKARSNLKT 79

Query: 68  LKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLHQNLVRRF 127
           LK LG  ++HGVD TT+  HP L +  FDR++FNFPHAGF   E ++  I+ H+ LV  F
Sbjct: 80  LKRLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKHRELVFGF 139

Query: 128 LRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPGYVNKKGS 187
              A  LL  NG++H++HK   P+SEW +EE+A    L L + V F   +YPGY NK+G 
Sbjct: 140 FNGASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYPGYENKRGD 199

Query: 188 GPNSNLTFPVGLCSTFKF 206
           G   +  F +G CSTFKF
Sbjct: 200 GRRCDQPFLLGECSTFKF 216

BLAST of Carg24731 vs. TAIR 10
Match: AT5G56060.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 167.5 bits (423), Expect = 1.2e-41
Identity = 94/189 (49.74%), Postives = 127/189 (67.20%), Query Frame = 0

Query: 9   ITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIKTTLEEL 68
           +  Y +   ILLVGEG+FSFS  LA  FG+ATN+ ATSLD+ E L  KY +D K  +E L
Sbjct: 9   LQQYSNKQKILLVGEGDFSFSLSLARVFGSATNITATSLDTREELGIKY-TDGKANVEGL 68

Query: 69  KELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQY-SEHETRQIKLHQNLVRRF 128
           +  GC+V+HGV+V +MS    L    +DRI+FNFPH+G  + SEH+   I LHQ LVR F
Sbjct: 69  ELFGCTVVHGVNVHSMSSDYRLGR--YDRIIFNFPHSGLGFGSEHDIFFIMLHQGLVRGF 128

Query: 129 LRNAKELLA-ENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPGYVNKKG 188
           L +A+++L  E+G+IH+THK + P++ W IE +A E+ L L   +EF+   +PGY NKKG
Sbjct: 129 LESARKMLKDEDGEIHVTHKTTDPFNRWGIETLAGEKGLRLIGEIEFHKWAFPGYSNKKG 188

Query: 189 SGPNSNLTF 196
            G N N TF
Sbjct: 189 GGSNCNSTF 194

BLAST of Carg24731 vs. TAIR 10
Match: AT5G56075.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 161.8 bits (408), Expect = 6.8e-40
Identity = 91/205 (44.39%), Postives = 132/205 (64.39%), Query Frame = 0

Query: 7   KWITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIKTTLE 66
           K + HY +   ILLVGEG+FSFS  LA AFG+A+N+ ATSLD++  L +K+ +  K  +E
Sbjct: 65  KRLRHYTNKQKILLVGEGDFSFSLSLARAFGSASNLTATSLDTQGELEQKFKNG-KANVE 124

Query: 67  ELKELGCSVMHGVDVTTMSQHPLL-CHTLFDRIVFNFPHAGFQYSEHETRQIKLHQNLVR 126
           EL+ LGCSV++GV+V +M   P +    ++DR++FNFP        HE         LVR
Sbjct: 125 ELERLGCSVVYGVNVHSMITKPSVGGSAIYDRVIFNFP-------THE---------LVR 184

Query: 127 RFLRNAKELLAE---NGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPGYV 186
            F+++A+ L+ +    G+IH+ HK  +P+SEW+++ + E+E L L   VEF +  YPGY 
Sbjct: 185 GFMKSARVLVKDEDKGGEIHVIHKTEYPFSEWKLKTLGEKEGLDLIREVEFCLSHYPGYF 244

Query: 187 NKKGSGPNSNLTFPVGLCSTFKFVK 208
           NK+GSG  S+ +FPVG  STF F K
Sbjct: 245 NKRGSGGYSDSSFPVGKSSTFMFTK 252

BLAST of Carg24731 vs. TAIR 10
Match: AT4G26485.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 152.9 bits (385), Expect = 3.1e-37
Identity = 82/171 (47.95%), Postives = 112/171 (65.50%), Query Frame = 0

Query: 41  NVVATSLDSEETLLRKYGSDIKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVF 100
           N+ ATSLDSE+ L  KY  D    +  LK  GC + H VDV TMS    L    +DRIVF
Sbjct: 2   NITATSLDSEDELSIKY-MDAVDNINILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVF 61

Query: 101 NFPHAGFQY--SEHETRQIKLHQNLVRRFLRNAKELLAENGDIHITHKISFPYSEWEIEE 160
           NFPHAG ++   E  +R I+ H+ LVR FL NAKE+L E+G+IHITHK ++P+S+W I++
Sbjct: 62  NFPHAGSRFFGRELSSRAIESHKELVRGFLENAKEMLEEDGEIHITHKTTYPFSDWGIKK 121

Query: 161 VAEEEDLFLRELVEFNIGDYPGYVNKKGS-GPNSNLTFPVGLCSTFKFVKT 209
           + + E L L +  +F +  YPGY+ K+GS G  S+  FPVG CST+ F ++
Sbjct: 122 LGKGEGLKLLKKSKFELSHYPGYITKRGSGGRRSDDYFPVGECSTYMFTQS 171

BLAST of Carg24731 vs. TAIR 10
Match: AT1G55800.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 136.0 bits (341), Expect = 4.0e-32
Identity = 84/229 (36.68%), Postives = 113/229 (49.34%), Query Frame = 0

Query: 8   WITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDS------------------ 67
           W+ HY S H ILLVGEG+FSFS  LA+ FG+A+N+ A+SLDS                  
Sbjct: 18  WVKHYSSKHQILLVGEGDFSFSCSLATCFGSASNIYASSLDSYDYKPVNKGCSFKLDFLS 77

Query: 68  ---------EETLLRKYGSDIKTTLEELKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVF 127
                     + ++RKY  + ++ LE LK LG  ++HGVD TT+  HP L +  FDR++F
Sbjct: 78  CCMSFMVIKPDDVVRKY-KNARSNLETLKRLGAFLLHGVDATTLHFHPDLRYRRFDRVIF 137

Query: 128 NFPHAGFQYSEHETRQIK----LHQNLVRRFLRNAKELLAENGDIHITHKISFPYSEWEI 187
           NFPH GF   E +  QI+      +NL + FL  A  +L  +G                 
Sbjct: 138 NFPHTGFHRKESDPCQIQPAAATLRNLFKDFLHGASHMLRADG----------------- 197

Query: 188 EEVAEEEDLFLRELVEFNIGDYPGYVNKKGSGPNSNLTFPVGLCSTFKF 206
                       EL  F   +YPGY NK+G G   +  F +G CSTFKF
Sbjct: 198 ------------ELEAFEKRNYPGYENKRGDGSRCDQPFLLGECSTFKF 216