Carg24691 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24691
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionras-related protein Rab7-like
LocationCarg_Chr11: 5964550 .. 5966974 (+)
RNA-Seq ExpressionCarg24691
SyntenyCarg24691
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTGAATTTGCAGGGTTGGGAAGACCTCTTTGATGAACCAGTATCCTGCTTGATCATTGAATTATAACTATGTTATAATGCAACATTTTCCATTCGAGTTTCTTTTTTTGTAGTATCAGTTTCTTGATTCTCCGTGTGGTTTTCTTTAGCTTTGAAAAACAGATATGTGAATAAGAAGTTTAGTAATCAATACAAGGCCACCATTGGAGCTGATTTCTTGACTAAAGAGGTGCAATTCGAAGATAGACTTTTCACTTTGCAGGTTGGTGGTGGGGAGACTTTAATTTGCCCATGAATATGCTCTTTTATTGCTCTAATTTGGTGAATTCCCTTGAATTGATAGTGATCAAAAAGTGCACAAAGGCACCTCAATCTAGTGTGTGTTCATATGGAAAATTTTATGAACTCGATTGATCCATTTGGGAAGATACCAATTTCTTGGATGACCTTTCGAAACGGATACTTGTGTTGTAATCATTTATGAGTAGCAAGAACATGGCTATGAAGGCTGCATTGTTTCGTGTATTGGACTCGGTTTAACCTTGAGTGATTAGATCCCATTTAGTAGCATTTCATTGCCATCTCCTGAGAGCTGTGGCATATAGGTTTAATGTATACCCATGAAGCCAACAAGCAACAAAGAGACTCGATATATCCTGTTAAATGTAATTTATTCCTTTCTGCCTTACAGATATGGGATACAGCAGGCCAGGAACGGTTCCAAAGTCTCGGTGTTGCTTTTTACCGAGGTGCTGATTGCTGCGTTCTGGTGTATGATGTCAATTCAATGAAATCATTTGACAACCTTAACAACTGGCGAGAAGAATTCCTTATACAGGTTTTTTCATCTGTGTTCATTGATTATCTCATTACCAGAAAGCCATTAGAAGAATTCAAATAAGTTGAAAAGAAACCTGAACATCTTTTGACATTTTTGTAAAACCATCTGCAGGCGAGCCCTTCAGACCCGGAAAATTTTCCCTTCGTTGTTTTAGGAAATAAAGTCGACGTTGATGGTGGAAACAGTAGAGTTGTAAGTATTACCTAATTTTCTATACTGTATCATTTCCTCTTTCTATCTCCAGAAGATAGTGAAATGTTCTGTTTTTCTGAATATTTCAGGTTTCAGAGAAAAAGGCACGAGCTTGGTGTGCGTCGAAAGGAAATATTCCATACTTTGAAACGTCTGCCAAAGAAGGCATTAACGTAGAAGAAGCTTTCGAATGCATTGCGAAAAATGCCCTCAAGAGTGGGGAAGAGGAAGAGATGTAAGTTCATATGCCAAACCATAATTTCTGAGAATCAAATATATAATTAACTCTGTCTTTCTGAATTCTATTCTCAGTTATTTGATGTACGCGTACATAAAATACGAAGCAAAGTAACTGTTCGTTACATAATCATGAAATTCACCCAGCAATAACAACTCGAGTTGATATAGGTGTTGATGTTGAGGATTGTTGGGAGAGGAGTCCCACATCGGCTAATAGGGGTTGATCATGGGTTTATAAGTAAAGAATACATTTACTTTGGTATGAGGCCTTTTGGGTAAACTAAAAGCATACCATTGTGGAGGGTCGCGGTTTCTAACATGGTATCGAGCCATGCCCTTAACTTAGCCATGTCAATAGAATCCTCAAATGTTGAAAAAAGAAGTTGTGAGCCCCAAAGGTGTAGTCAAAAGTGACTAAAGTGTCAAACAAAGGGTGTACTTTGTTCGAGGGTTCTAGAGAAGAAGTCGAGCATCAATTAAGGGGAGGCTGTTCGAGGGCTCCATAGGCCTCAGGGGAGGCTCTATGGTGTACTATGTTCAAGGGGAGGATTGTTGAGGATTGTTGGGAGAGGAGTCCCACAAGGATTGATCGGGAGTTTATAAGTAAGGAATACTATCTCCATTGATATGAGGTTTTTTGGGTAAACAAAAAATAAAGTCATGAGAGCTTATGCTCAAAGTGGACAATATCATATCATACTATCGTGGAGAGTCGTGATTCCTAACAGTCGATTGGGAAAACTCACCTGATTTTTTGGTTCCAAGATTGGCATGTGCTTATTGCATGTCGTAGATTGGCAGCATGTGGGCCGGATCCCAAACGCATCGTATAGCTTATAGCGAACATAACGCTGAGCTATAGTTTAGCTCTAGCAGTAGAATTAGTTTGTAATTTTGACCCGAGGCTGTGGATATGTAGAATCTAGTTCTTCCTAAATGGCTGCGTTTCCTAATTGCTCTTCAAAATTCTCTAACTTCGTTGCTTCAAACTTTGTATCATCAATGCTTCATTGGTCCACCTTCTGGCTGTTAACTTTCACTTTCACAAAACCTTTGTTGATTGATGGTTGCCATGTTATTGCAGATACTTACCCGACACGATCGATGTTGCAACCAACAATCAGTCAAGATCATCTGGATGCGACTGCTGA

mRNA sequence

CTTTGAATTTGCAGGGTTGGGAAGACCTCTTTGATGAACCAATATGTGAATAAGAAGTTTAGTAATCAATACAAGGCCACCATTGGAGCTGATTTCTTGACTAAAGAGGTGCAATTCGAAGATAGACTTTTCACTTTGCAGATATGGGATACAGCAGGCCAGGAACGGTTCCAAAGTCTCGGTGTTGCTTTTTACCGAGGTGCTGATTGCTGCGTTCTGGTGTATGATGTCAATTCAATGAAATCATTTGACAACCTTAACAACTGGCGAGAAGAATTCCTTATACAGGCGAGCCCTTCAGACCCGGAAAATTTTCCCTTCGTTGTTTTAGGAAATAAAGTCGACGTTGATGGTGGAAACAGTAGAGTTGTTTCAGAGAAAAAGGCACGAGCTTGGTGTGCGTCGAAAGGAAATATTCCATACTTTGAAACGTCTGCCAAAGAAGGCATTAACGTAGAAGAAGCTTTCGAATGCATTGCGAAAAATGCCCTCAAGAGTGGGGAAGAGGAAGAGATATACTTACCCGACACGATCGATGTTGCAACCAACAATCAGTCAAGATCATCTGGATGCGACTGCTGA

Coding sequence (CDS)

ATGAACCAATATGTGAATAAGAAGTTTAGTAATCAATACAAGGCCACCATTGGAGCTGATTTCTTGACTAAAGAGGTGCAATTCGAAGATAGACTTTTCACTTTGCAGATATGGGATACAGCAGGCCAGGAACGGTTCCAAAGTCTCGGTGTTGCTTTTTACCGAGGTGCTGATTGCTGCGTTCTGGTGTATGATGTCAATTCAATGAAATCATTTGACAACCTTAACAACTGGCGAGAAGAATTCCTTATACAGGCGAGCCCTTCAGACCCGGAAAATTTTCCCTTCGTTGTTTTAGGAAATAAAGTCGACGTTGATGGTGGAAACAGTAGAGTTGTTTCAGAGAAAAAGGCACGAGCTTGGTGTGCGTCGAAAGGAAATATTCCATACTTTGAAACGTCTGCCAAAGAAGGCATTAACGTAGAAGAAGCTTTCGAATGCATTGCGAAAAATGCCCTCAAGAGTGGGGAAGAGGAAGAGATATACTTACCCGACACGATCGATGTTGCAACCAACAATCAGTCAAGATCATCTGGATGCGACTGCTGA

Protein sequence

MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARAWCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGCDC
Homology
BLAST of Carg24691 vs. NCBI nr
Match: KAG7022292.1 (Ras-related protein RABG3f [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 377.5 bits (968), Expect = 6.6e-101
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA
Sbjct: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC
Sbjct: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180

Query: 181 DC 183
           DC
Sbjct: 181 DC 182

BLAST of Carg24691 vs. NCBI nr
Match: XP_022931294.1 (ras-related protein RABG3f [Cucurbita moschata] >KAG6588442.1 Ras-related protein RABG3f, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377.5 bits (968), Expect = 6.6e-101
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA
Sbjct: 85  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC
Sbjct: 145 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 204

Query: 181 DC 183
           DC
Sbjct: 205 DC 206

BLAST of Carg24691 vs. NCBI nr
Match: XP_023529453.1 (ras-related protein RABG3f isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375.6 bits (963), Expect = 2.5e-100
Identity = 181/182 (99.45%), Postives = 181/182 (99.45%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA
Sbjct: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQ RSSGC
Sbjct: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQRRSSGC 180

Query: 181 DC 183
           DC
Sbjct: 181 DC 182

BLAST of Carg24691 vs. NCBI nr
Match: XP_023529452.1 (ras-related protein RABG3f isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375.6 bits (963), Expect = 2.5e-100
Identity = 181/182 (99.45%), Postives = 181/182 (99.45%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA
Sbjct: 85  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQ RSSGC
Sbjct: 145 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQRRSSGC 204

Query: 181 DC 183
           DC
Sbjct: 205 DC 206

BLAST of Carg24691 vs. NCBI nr
Match: XP_022971474.1 (ras-related protein Rab7-like [Cucurbita maxima])

HSP 1 Score: 373.2 bits (957), Expect = 1.2e-99
Identity = 180/182 (98.90%), Postives = 180/182 (98.90%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA
Sbjct: 85  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCA KGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQ RSSGC
Sbjct: 145 WCALKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQPRSSGC 204

Query: 181 DC 183
           DC
Sbjct: 205 DC 206

BLAST of Carg24691 vs. ExPASy Swiss-Prot
Match: Q9LS94 (Ras-related protein RABG3f OS=Arabidopsis thaliana OX=3702 GN=RABG3F PE=1 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 1.0e-96
Identity = 167/182 (91.76%), Postives = 177/182 (97.25%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSF+NLNNWREEFLIQASPSDPENFPFV++GNKVDVD GNSRVVSEKKA+A
Sbjct: 85  VLVYDVNSMKSFENLNNWREEFLIQASPSDPENFPFVLIGNKVDVDDGNSRVVSEKKAKA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAK G NVEEAF+CIAK+ALKSGEEEE+YLPDTIDV T+NQ RS+GC
Sbjct: 145 WCASKGNIPYFETSAKVGTNVEEAFQCIAKDALKSGEEEELYLPDTIDVGTSNQQRSTGC 204

Query: 181 DC 183
           +C
Sbjct: 205 EC 206

BLAST of Carg24691 vs. ExPASy Swiss-Prot
Match: P31022 (Ras-related protein Rab7 OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 3.0e-96
Identity = 165/182 (90.66%), Postives = 178/182 (97.80%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNS+KSFDNLNNWREEFLIQA+PSDPENFPFVV+GNK+D+DGGNSRVVSEKKARA
Sbjct: 85  VLVYDVNSVKSFDNLNNWREEFLIQANPSDPENFPFVVIGNKIDIDGGNSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCA+KGNIPYFETSAKEGINVEEAF+ IAK+ALKSGEEEE+YLPDTIDV  ++Q RS+GC
Sbjct: 145 WCAAKGNIPYFETSAKEGINVEEAFQTIAKDALKSGEEEELYLPDTIDVGNSSQPRSTGC 204

Query: 181 DC 183
           +C
Sbjct: 205 EC 206

BLAST of Carg24691 vs. ExPASy Swiss-Prot
Match: Q9XI98 (Ras-related protein RABG3e OS=Arabidopsis thaliana OX=3702 GN=RABG3E PE=2 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 2.4e-93
Identity = 161/182 (88.46%), Postives = 173/182 (95.05%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNS KSF++LNNWREEFLIQASPSDPENFPFVV+GNK+DVDGG+SRVVSEKKARA
Sbjct: 85  VLVYDVNSAKSFEDLNNWREEFLIQASPSDPENFPFVVIGNKIDVDGGSSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPY+ETSAK G NVE+AF CI  NA+KSGEEEE+YLPDTIDV T+N  RS+GC
Sbjct: 145 WCASKGNIPYYETSAKVGTNVEDAFLCITTNAMKSGEEEEMYLPDTIDVGTSNPQRSTGC 204

Query: 181 DC 183
           +C
Sbjct: 205 EC 206

BLAST of Carg24691 vs. ExPASy Swiss-Prot
Match: O24461 (Ras-related protein Rab7 OS=Prunus armeniaca OX=36596 PE=2 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 6.9e-93
Identity = 163/183 (89.07%), Postives = 173/183 (94.54%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVN+KFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNRKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVN MKSF+NLNNWREEFLIQA+PSDPENFPFVVLGNK+DVDGGNSRVVSEKKA+A
Sbjct: 85  VLVYDVNVMKSFENLNNWREEFLIQATPSDPENFPFVVLGNKIDVDGGNSRVVSEKKAKA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVA-TNNQSRSSG 180
           WCASKGNIPYFETSAKEG NV++AF+CIAKNAL +  EEEIYLPDTIDVA    Q RSSG
Sbjct: 145 WCASKGNIPYFETSAKEGFNVDDAFQCIAKNALNNEPEEEIYLPDTIDVAGGGRQQRSSG 204

Query: 181 CDC 183
           C+C
Sbjct: 205 CEC 207

BLAST of Carg24691 vs. ExPASy Swiss-Prot
Match: Q40787 (Ras-related protein Rab7 OS=Cenchrus ciliaris OX=35872 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 2.0e-92
Identity = 162/181 (89.50%), Postives = 173/181 (95.58%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSFDNLNNWREEFLIQASPSDP+NFPFV+LGNKVDVDGGNSRVVSEKKA+A
Sbjct: 85  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVLLGNKVDVDGGNSRVVSEKKAKA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQS-RSSG 180
           WCASKGNIPYFETSAKEG NVE+AF+CI KNALK+  EEE+Y+PDT+DV   N++ RSSG
Sbjct: 145 WCASKGNIPYFETSAKEGTNVEDAFQCIVKNALKNEPEEELYVPDTVDVVGGNRAQRSSG 204

BLAST of Carg24691 vs. ExPASy TrEMBL
Match: A0A6J1ETW2 (ras-related protein RABG3f OS=Cucurbita moschata OX=3662 GN=LOC111437519 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 3.2e-101
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA
Sbjct: 85  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC
Sbjct: 145 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 204

Query: 181 DC 183
           DC
Sbjct: 205 DC 206

BLAST of Carg24691 vs. ExPASy TrEMBL
Match: A0A6J1I8N5 (ras-related protein Rab7-like OS=Cucurbita maxima OX=3661 GN=LOC111470179 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 6.0e-100
Identity = 180/182 (98.90%), Postives = 180/182 (98.90%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA
Sbjct: 85  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCA KGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQ RSSGC
Sbjct: 145 WCALKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQPRSSGC 204

Query: 181 DC 183
           DC
Sbjct: 205 DC 206

BLAST of Carg24691 vs. ExPASy TrEMBL
Match: A0A6J1I650 (ras-related protein Rab7 OS=Cucurbita maxima OX=3661 GN=LOC111471043 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 7.9e-100
Identity = 179/182 (98.35%), Postives = 180/182 (98.90%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVD+DGGNSRVVSEKKARA
Sbjct: 85  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDIDGGNSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVA NNQ RSSGC
Sbjct: 145 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVARNNQPRSSGC 204

Query: 181 DC 183
           DC
Sbjct: 205 DC 206

BLAST of Carg24691 vs. ExPASy TrEMBL
Match: A0A6J1GK85 (ras-related protein Rab7 OS=Cucurbita moschata OX=3662 GN=LOC111455038 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 7.9e-100
Identity = 179/182 (98.35%), Postives = 180/182 (98.90%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVD+DGGNSRVVSEKKARA
Sbjct: 85  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDIDGGNSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVA NNQ RSSGC
Sbjct: 145 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVARNNQPRSSGC 204

Query: 181 DC 183
           DC
Sbjct: 205 DC 206

BLAST of Carg24691 vs. ExPASy TrEMBL
Match: Q6UNT4 (Putative GTP-binding protein OS=Cucumis sativus OX=3659 GN=Rab7b PE=2 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.3e-99
Identity = 178/182 (97.80%), Postives = 180/182 (98.90%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA
Sbjct: 85  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAKEGINVEEAF+CIAKNALKSGEEEEIYLPDTIDV +NNQ RSSGC
Sbjct: 145 WCASKGNIPYFETSAKEGINVEEAFQCIAKNALKSGEEEEIYLPDTIDVGSNNQPRSSGC 204

Query: 181 DC 183
           DC
Sbjct: 205 DC 206

BLAST of Carg24691 vs. TAIR 10
Match: AT3G18820.1 (RAB GTPase homolog G3F )

HSP 1 Score: 354.0 bits (907), Expect = 7.3e-98
Identity = 167/182 (91.76%), Postives = 177/182 (97.25%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNSMKSF+NLNNWREEFLIQASPSDPENFPFV++GNKVDVD GNSRVVSEKKA+A
Sbjct: 85  VLVYDVNSMKSFENLNNWREEFLIQASPSDPENFPFVLIGNKVDVDDGNSRVVSEKKAKA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAK G NVEEAF+CIAK+ALKSGEEEE+YLPDTIDV T+NQ RS+GC
Sbjct: 145 WCASKGNIPYFETSAKVGTNVEEAFQCIAKDALKSGEEEELYLPDTIDVGTSNQQRSTGC 204

Query: 181 DC 183
           +C
Sbjct: 205 EC 206

BLAST of Carg24691 vs. TAIR 10
Match: AT1G49300.1 (RAB GTPase homolog G3E )

HSP 1 Score: 342.8 bits (878), Expect = 1.7e-94
Identity = 161/182 (88.46%), Postives = 173/182 (95.05%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNS KSF++LNNWREEFLIQASPSDPENFPFVV+GNK+DVDGG+SRVVSEKKARA
Sbjct: 85  VLVYDVNSAKSFEDLNNWREEFLIQASPSDPENFPFVVIGNKIDVDGGSSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPY+ETSAK G NVE+AF CI  NA+KSGEEEE+YLPDTIDV T+N  RS+GC
Sbjct: 145 WCASKGNIPYYETSAKVGTNVEDAFLCITTNAMKSGEEEEMYLPDTIDVGTSNPQRSTGC 204

Query: 181 DC 183
           +C
Sbjct: 205 EC 206

BLAST of Carg24691 vs. TAIR 10
Match: AT1G49300.2 (RAB GTPase homolog G3E )

HSP 1 Score: 342.8 bits (878), Expect = 1.7e-94
Identity = 161/182 (88.46%), Postives = 173/182 (95.05%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVNS KSF++LNNWREEFLIQASPSDPENFPFVV+GNK+DVDGG+SRVVSEKKARA
Sbjct: 85  VLVYDVNSAKSFEDLNNWREEFLIQASPSDPENFPFVVIGNKIDVDGGSSRVVSEKKARA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPY+ETSAK G NVE+AF CI  NA+KSGEEEE+YLPDTIDV T+N  RS+GC
Sbjct: 145 WCASKGNIPYYETSAKVGTNVEDAFLCITTNAMKSGEEEEMYLPDTIDVGTSNPQRSTGC 204

Query: 181 DC 183
           +C
Sbjct: 205 EC 206

BLAST of Carg24691 vs. TAIR 10
Match: AT1G52280.1 (RAB GTPase homolog G3D )

HSP 1 Score: 334.3 bits (856), Expect = 6.0e-92
Identity = 158/182 (86.81%), Postives = 167/182 (91.76%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQ+VN+KFSNQYKATIGADFLTKEVQ +DR+FTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQFVNRKFSNQYKATIGADFLTKEVQIDDRIFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLVYDVN MKSFDNLNNWREEFLIQASPSDPENFPFVVLGNK DVDGG SRVVSEKKA+A
Sbjct: 85  VLVYDVNVMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKTDVDGGKSRVVSEKKAKA 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAKEG NV+ AFECI KNA K+  EEE YLPDTIDVA   Q RS+GC
Sbjct: 145 WCASKGNIPYFETSAKEGFNVDAAFECITKNAFKNEPEEEPYLPDTIDVAGGQQQRSTGC 204

Query: 181 DC 183
           +C
Sbjct: 205 EC 206

BLAST of Carg24691 vs. TAIR 10
Match: AT3G16100.1 (RAB GTPase homolog G3C )

HSP 1 Score: 331.6 bits (849), Expect = 3.9e-91
Identity = 155/182 (85.16%), Postives = 170/182 (93.41%), Query Frame = 0

Query: 1   MNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCC 60
           MNQ+VN+KFSNQYKATIGADFLTKEVQ +DR+FTLQIWDTAGQERFQSLGVAFYRGADCC
Sbjct: 25  MNQFVNRKFSNQYKATIGADFLTKEVQIDDRIFTLQIWDTAGQERFQSLGVAFYRGADCC 84

Query: 61  VLVYDVNSMKSFDNLNNWREEFLIQASPSDPENFPFVVLGNKVDVDGGNSRVVSEKKARA 120
           VLV DVN MKSF+NLNNWREEFLIQASPSDPENFPFVVLGNK DVDGG SRVV+EKKA++
Sbjct: 85  VLVNDVNVMKSFENLNNWREEFLIQASPSDPENFPFVVLGNKTDVDGGKSRVVTEKKAKS 144

Query: 121 WCASKGNIPYFETSAKEGINVEEAFECIAKNALKSGEEEEIYLPDTIDVATNNQSRSSGC 180
           WCASKGNIPYFETSAK+G+NV+ AFECIAKNALK+  EEE+YLPDTIDVA   Q RS+GC
Sbjct: 145 WCASKGNIPYFETSAKDGVNVDAAFECIAKNALKNEPEEEVYLPDTIDVAGARQQRSTGC 204

Query: 181 DC 183
           +C
Sbjct: 205 EC 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022292.16.6e-101100.00Ras-related protein RABG3f [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022931294.16.6e-101100.00ras-related protein RABG3f [Cucurbita moschata] >KAG6588442.1 Ras-related protei... [more]
XP_023529453.12.5e-10099.45ras-related protein RABG3f isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023529452.12.5e-10099.45ras-related protein RABG3f isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022971474.11.2e-9998.90ras-related protein Rab7-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LS941.0e-9691.76Ras-related protein RABG3f OS=Arabidopsis thaliana OX=3702 GN=RABG3F PE=1 SV=1[more]
P310223.0e-9690.66Ras-related protein Rab7 OS=Pisum sativum OX=3888 PE=2 SV=1[more]
Q9XI982.4e-9388.46Ras-related protein RABG3e OS=Arabidopsis thaliana OX=3702 GN=RABG3E PE=2 SV=1[more]
O244616.9e-9389.07Ras-related protein Rab7 OS=Prunus armeniaca OX=36596 PE=2 SV=1[more]
Q407872.0e-9289.50Ras-related protein Rab7 OS=Cenchrus ciliaris OX=35872 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ETW23.2e-101100.00ras-related protein RABG3f OS=Cucurbita moschata OX=3662 GN=LOC111437519 PE=4 SV... [more]
A0A6J1I8N56.0e-10098.90ras-related protein Rab7-like OS=Cucurbita maxima OX=3661 GN=LOC111470179 PE=4 S... [more]
A0A6J1I6507.9e-10098.35ras-related protein Rab7 OS=Cucurbita maxima OX=3661 GN=LOC111471043 PE=4 SV=1[more]
A0A6J1GK857.9e-10098.35ras-related protein Rab7 OS=Cucurbita moschata OX=3662 GN=LOC111455038 PE=4 SV=1[more]
Q6UNT42.3e-9997.80Putative GTP-binding protein OS=Cucumis sativus OX=3659 GN=Rab7b PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G18820.17.3e-9891.76RAB GTPase homolog G3F [more]
AT1G49300.11.7e-9488.46RAB GTPase homolog G3E [more]
AT1G49300.21.7e-9488.46RAB GTPase homolog G3E [more]
AT1G52280.16.0e-9286.81RAB GTPase homolog G3D [more]
AT3G16100.13.9e-9185.16RAB GTPase homolog G3C [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00449RASTRNSFRMNGcoord: 92..105
score: 40.9
coord: 8..24
score: 35.89
coord: 130..152
score: 43.46
coord: 26..48
score: 47.03
NoneNo IPR availableSMARTSM00173ras_sub_4coord: 1..155
e-value: 7.9E-12
score: 49.6
NoneNo IPR availableSMARTSM00175rab_sub_5coord: 1..155
e-value: 2.0E-73
score: 260.0
NoneNo IPR availableSMARTSM00176ran_sub_2coord: 1..181
e-value: 0.0024
score: -0.4
NoneNo IPR availablePANTHERPTHR47981:SF11BNAA01G26470D PROTEINcoord: 1..182
NoneNo IPR availablePANTHERPTHR47981RAB FAMILYcoord: 1..182
NoneNo IPR availablePROSITEPS51419RABcoord: 1..182
score: 28.925571
NoneNo IPR availableCDDcd01862Rab7coord: 1..158
e-value: 6.60556E-118
score: 329.24
IPR001806Small GTPaseSMARTSM00174rho_sub_3coord: 1..155
e-value: 3.2E-9
score: -0.8
IPR001806Small GTPasePFAMPF00071Rascoord: 2..153
e-value: 7.9E-47
score: 158.9
IPR001806Small GTPasePROSITEPS51421RAScoord: 1..182
score: 15.386481
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 1..165
e-value: 1.1E-57
score: 196.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 1..158
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 2..148
e-value: 3.4E-18
score: 63.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24691-RACarg24691-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding