Carg24606 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24606
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPhenylalanyl-tRNA synthetase
LocationCarg_Chr03: 10193263 .. 10198601 (+)
RNA-Seq ExpressionCarg24606
SyntenyCarg24606
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACGAAGACAATGACATCGACTCTGTCATTTACTCGACCTTTTCTCTTCACTAATGCTTCTAATCTTCTTCGCAGAAACTGCCTCGCAACCTTTTCCTTCTCTGCATCTTTCTCCTCCGACAGCCTTCACCAGAAGAAATGGAGGCAGCCGGTTTCTTCACTTCTTGAGCTTGGCGGAGTCGCTATTGGGAAACATGGTCAACTATTCTTTCTTCTTCTATTTTATTTGTCTATCTCGTTTTATTGTTTTCATAATCCGCTAATGTATTCTGCGGCACGGATTTATTCCTATTTGTGAGATTTTGTGGTTGAACTTCCGCGATTTCGTTGAATTTATTGGTTTTAAGAGCGAGTTCTTTTTTTTTTTTTTTTGTCTTTATTCAATTTCTTGTTGATGTTAACTTAAGAATATAATCGAGAGAATTAAGCTAAAGAGATGGGTACAGCAGCTGTGCATATTTCTAATTTCTAAGGAGAAATAACTTTTAAGCTTGGTTCTGTCATTCTGAGTCATAATGTATCCACTTCTTGGATTCCACTTTTATTTTGGCGTTGAGGAGATATAACCACTTCCAAAAATGTTTCTCAGTTGTTATGTTGCTTCATTTTCCCCCCGATTTGAAGTTGTATAACATTTCATTACATTTCACATTTCTCTTTTGTTAACCCATCCAAAGTCTTAAGGATTTAATTTTCTAGGTGCTTGTTGAACTTTGTTGTCAATAAACATTTTTTTTTTTCGAATTTTTGCCGTAGAAGTTCAATTTTAATGTGATTGCTTTGCTATATTATTGGAGGCTTGCAGATGTTGTGAGGGATGATCCCACGAACAATGTCCCAGATAGTATTTTTTCAAAACTTGGTATGCAACTCCACCAAAGAGATAACCATCCACTTGGCATTCTGAAGAATGAGATATACAACTATTTTGATACAAATTACTCAAATAAGTTCACTAAGTTTGATGACCTCAGTCCAATCGTTTCTGTGAAGGAGGTAAGCCTTTTAAACTCATCAATTAGTTTTGAGGCTCAAATGAAGCTCATTCTATGACTATCTTTATGCCTGCGGAAATTTTAGTTTTCAGAAATGGATTAGTCCCGTAGCCAATTCGTTGAAAAATGGGTTTTACGCTGCTATAGAGCCTCTGAATTTGATATACCGCATTCTGATTAATATATCAGAGTGATTGAAGAATCTTTGATCTAGAAGGAACTAATGACAGAAAACATATGAAGGATAAAGAAATTGTGGCGTCCAGTGGTCCAAATAAGCTCATATGAAAGAGCATCTTCTGATCTCATGAAGTTCACTAACTTAACACCCTTGTATTCATTTGACTCACTAATAACCTTTTGATTTACATCTTTATTTTCTATGGAGGCCAATGTGGTCAATTTGGAATTATCAATTTTCTCTCTAGAGTAATAATAACATATTTGAAACCAATTTCAAACTTGATGGATTAATTAAAAGTTAAATGCAAATCTCGAAGACCAAGTGTATCACTTCTGAAATAACTAAACAGAAACTACATTCAAACTTTAAGGACCAAAAAGTAATTCAAATCTTGGGACCAAACACCTTAAGGACCAAATAGAACTAAATTTAATGTTCACGGACCAAAAGTTTTGGCTTTGAAATCTCAGGAACAATTAGAAGCTAAGTTCAATACTAAGGGACCGAAAGTGTATTTTTTCAAAAAAAAAAGAAACTTTCATTCTTTTCGTGATTTTTATTGAAGTACTGAACTTATTTTGGAAGATGGCGGGATCGGTTTGGTATTTGTCAACTTTCCTGTTGACTGGTCAAATTTTTTCCCTAAATTCCAATAGCTTATCGTGTTGTTTATTTATTAATTAATCATTTTATAATAACAATTATTTTTAAACATACAAAGCCTCTTGTATCTGAATCCTATAATTATGTCTGCAGAATTTTGATGATGTGTTGGTTCCAGAAGATCATGTAAGCCGAAGTTATAATGATACATACTACATTGACTCGCAAACTGTTCTGAGATGTCATACAAGTGCTCACCAAGCGGAGCTGTTGAGAAGAGGCTATACTCAGTTTCTTGTGACAGGCGATGTTTATCGTCGGGATTCCATTGATTCAACTCATTACCCTGTATTTCATCAGGTAATCTAAATAAGTTTATTAATCATGTCATTTGCTAAATTTACCATAGTTTTCACTTAATGAATTTCTTTGCCTACTTCTCAGATGGAAGGTGTCCGTGTTTTTTCTCTGAATGATTGGGAGGCATCTGGTACAGATGGAACATCTTTTGCAGCTGACGACTTGAAGAAATGTTTGGAAGGTTTGGCAAGGCATTTATTTGGTAAGAGTTGTGTATGAATTAACCCATGATTCTTCCCTTTCTATCTTAGTTATGGTTGGGGATTCTATTGAAAATTCATATGGGAATACAAACGATTTTTGGGTAGAGACAAAACAATAGCACTTGCAGAATAATCGGTTAAAAGACACAAACTGTAGAAGTAATAAGTATAATGTATTGGATAAGTATTAATTATTTTTATTTTGCATATGATTATTATAAAAACTTATTCCAAATGTAGTTACATTTTTAAGATAAAAACGAAAAATAGATTCCCAACTTACGTAATTAGGCTTTGATTTTTTAAAGTAATTTCTGTAATTTTGCAGTGGTTGTACACATTATGCAGCTATGTTATTATAAACTGATGTATTAAAATTTCCAGGTGCTGTGGAAATGCGCTGGGTTGATACATATTTCCCATTTACAAATCCATCATTTGAACTTGAGATTTTCTTTCAGGTACGTCAGATGATCGTTTAGTCAATTTGCTCTTCAGAACTCAATAATTTGATTATGGTACACTGCATTACTTCTTTTATGAATGACTTTCATTGCCGCCGATATATATATATATATATATATATATATAAGTTTAAATATGTCAAGTTTAATTCCATTTACACAATGGCATTTCTGTTTTATCATATATGAACTTTAAATAGAACCTAGATGATAACCTTACAACTTTTTGACTTTGATAAGTTAGACGAGGGAACCAAGAAGTGAAAACGTGCTTGGGAGGGGACAGATGTTTTTCTGTATTTTTGGGTGTGTGTGGGTGGGGGGTTATTTTGGGATAAAGAAACTTTCATTATCCAAAAACAATACAAAGATGATAAGTTATCAGCCTGACATAGGCAAGAGGGTAACAAAAGGTTCCTCTAATTAGGATTGGCCACAACCATTTCTCAGCTATTATTTCCGCCCATGAATCCTATAAAAGAATGAATGGATGATATTTGAATTATGAGATTTCCTCGTCAAATTATTTACATTGCATTTGCTGTTAGTTCCTTCTTTGGATCTGTAGTTGTTTTCTCTCTTTCTGTTCAACAAAAAATATAATAAAAAGTAATCCTAAAATTTGAGGGCATTTGATGTCCTACCCACGCAGGAGAAATGGCTGGAAGTATTGGGATGCGGGGTGATGGAGCAAGAAATATTGAAAAGAAGTGATAAAGAAAATAATGTTGCATGGGCTTTTGGTCTCGGGTTGGAAAGGCTAGCTATGGTTCTTTTTGATATACCTGATATCCGATTATTTTGGTCAGCTGACGAGCGATTTACCACGCAGGTACCTAATTGCACCACTTAAACTATACTCTTTCATTTTTTCCACTCTGTTTTCTCTTTGAAAATAAATTATCCAATCATCACGTTGGGCTTTTTATTTGCAGTTCTCAAAGGGCAAGCTAGGTGTCAAGTTTAAATCGTTTTCTAAGGTACTTTTACCAAACATTTTAGGCATGAAGGGATAACAATCTTCCGTTTCCTGAGAGAGCATATAATTCTATTTCTTTTAGTAACATCGAACTAGTTTCAATATGCTTTCTAACAAATTACATCTTCTAAATTATTTTAAAAAACTTTAGTAATTCAATCTGCTGCAGTTTCCACCTTGCTACAAGGACATCAGTTTTTGGATCAATGATTCCTTCACCGAGAATAATTTATGTGAAGTTGTTAGAGGAGTTGCCGGCGACCTTGTTGAAGAGGTATTTTATTAACTGTTTTCAGCGCATATATAATTCTTAAGATAATTTTAATGAAATGCTTAAGGTAGGGGCATGAGAACCTTTTTCTTCTCGAAATACATGCTTGAGAAAATCTGCTATCTGAAGTTCAATTTTTTAAAAGATAAAGTTGGTTGTGTAAATCTGAAAACGTTCTTTCAATCATGGATACTCTTTCAATTAACCTGGTCTGTTAGTTTCCGGAAAGTTGCACCTCGTTTAATGAAGTCATTTTAATTTTTTACAAATGGATGCTGGCAGGTACAACTGATAGACAGTTTTACCAACAAGAAGAAAGGGATGACGAGTCACTGTTACAGGATTGCTTACCGATCTATGGAACGGTCACTCACAGATGAAGAAATCAATGATTTGCAGGTATTTATCAACTTTAAGTTATATCCCTATTATATATTAACTTGTTTGAAAAAGAAGTTTGGATACAGCCTTCATATATTTTGTGGTTGCAAATGCAGTGGAACGTGAGAGAACAGGTTCAGAGCAAGTTGAACGTTGTCTTGCGATGAACATTGGAAGTGCAACAATATCAGTCCAAGCCATGTAATGTTATTTTTTACCTTCTCTACAGATTGAATTTTACTCTGTACACCATTTGAATTTTGCCTACAAAATTCTTCTAACTGGGTGATAGGAAGTGTAGAGTATATAACGTAACCAGTTGAAACCCTGAAAATAATCATCAAGAATTCAAGTGGAGATGTGCTGCAACTAATTTTTTAACATATACTATGAGTTTCTTTCCCCTACCTCATGTTTTAACATGACTTTGCTGATTTTTGGACCTGTTGAACTTATGCTATCAAGAAACTCCATTTACCATTGGACCACTTTTGCCTAGATTAGAAAGGGAGACCTCACTTTGTCTGAAATATATGACAAAGGAATAAGAATGTGTGCTTTGAAGTAAGTTGTGCCTATTTTCTTGACATCCGAACAAATAGAAGCTGAGGGAAGGTACTTTTCCTGAATAGTTGAGCCAATTTTGATATTTCTTTAGAAACTGACGAAGCCTGCAGGTTAAAATGCTGTACTTAGAAAATGTGCAAACCTTTAACAACACATAAAATAACCAAACTTGAACTAGAGCAAGATAATCTAGTGGTCAAAATGATGTCACGATAGACATCAACCCAAAGAAATAAAAGAACGAATATCATTTTCCAACACCCCTTCCAACTACCGATTTACCTTGCAATTCTAGTAAATATGATAAAGACCTCTTATGATATGAGATTCCC

mRNA sequence

GACGAAGACAATGACATCGACTCTGTCATTTACTCGACCTTTTCTCTTCACTAATGCTTCTAATCTTCTTCGCAGAAACTGCCTCGCAACCTTTTCCTTCTCTGCATCTTTCTCCTCCGACAGCCTTCACCAGAAGAAATGGAGGCAGCCGGTTTCTTCACTTCTTGAGCTTGGCGGAGTCGCTATTGGGAAACATGATGTTGTGAGGGATGATCCCACGAACAATGTCCCAGATAGTATTTTTTCAAAACTTGGTATGCAACTCCACCAAAGAGATAACCATCCACTTGGCATTCTGAAGAATGAGATATACAACTATTTTGATACAAATTACTCAAATAAGTTCACTAAGTTTGATGACCTCAGTCCAATCGTTTCTGTGAAGGAGAATTTTGATGATGTGTTGGTTCCAGAAGATCATGTAAGCCGAAGTTATAATGATACATACTACATTGACTCGCAAACTGTTCTGAGATGTCATACAAGTGCTCACCAAGCGGAGCTGTTGAGAAGAGGCTATACTCAGTTTCTTGTGACAGGCGATGTTTATCGTCGGGATTCCATTGATTCAACTCATTACCCTGTATTTCATCAGATGGAAGGTGTCCGTGTTTTTTCTCTGAATGATTGGGAGGCATCTGGTACAGATGGAACATCTTTTGCAGCTGACGACTTGAAGAAATGTTTGGAAGGTTTGGCAAGGCATTTATTTGGTGCTGTGGAAATGCGCTGGGTTGATACATATTTCCCATTTACAAATCCATCATTTGAACTTGAGATTTTCTTTCAGGAGAAATGGCTGGAAGTATTGGGATGCGGGGTGATGGAGCAAGAAATATTGAAAAGAAGTGATAAAGAAAATAATGTTGCATGGGCTTTTGGTCTCGGGTTGGAAAGGCTAGCTATGGTTCTTTTTGATATACCTGATATCCGATTATTTTGGTCAGCTGACGAGCGATTTACCACGCAGTTCTCAAAGGGCAAGCTAGGTGTCAAGTTTAAATCGTTTTCTAAGTTTCCACCTTGCTACAAGGACATCAGTTTTTGGATCAATGATTCCTTCACCGAGAATAATTTATGTGAAGTTGTTAGAGGAGTTGCCGGCGACCTTGTTGAAGAGGTACAACTGATAGACAGTTTTACCAACAAGAAGAAAGGGATGACGAGTCACTGTTACAGGATTGCTTACCGATCTATGGAACGGTCACTCACAGATGAAGAAATCAATGATTTGCAGTGGAACGTGAGAGAACAGGTTCAGAGCAAGTTGAACGTTGTCTTGCGATGAACATTGGAAGTGCAACAATATCAGTCCAAGCCATGTAATGTTATTTTTTACCTTCTCTACAGATTGAATTTTACTCTGTACACCATTTGAATTTTGCCTACAAAATTCTTCTAACTGGGTGATAGGAAGTGTAGAGTATATAACGTAACCAGTTGAAACCCTGAAAATAATCATCAAGAATTCAAGTGGAGATGTGCTGCAACTAATTTTTTAACATATACTATGAGTTTCTTTCCCCTACCTCATGTTTTAACATGACTTTGCTGATTTTTGGACCTGTTGAACTTATGCTATCAAGAAACTCCATTTACCATTGGACCACTTTTGCCTAGATTAGAAAGGGAGACCTCACTTTGTCTGAAATATATGACAAAGGAATAAGAATGTGTGCTTTGAAGTAAGTTGTGCCTATTTTCTTGACATCCGAACAAATAGAAGCTGAGGGAAGGTACTTTTCCTGAATAGTTGAGCCAATTTTGATATTTCTTTAGAAACTGACGAAGCCTGCAGGTTAAAATGCTGTACTTAGAAAATGTGCAAACCTTTAACAACACATAAAATAACCAAACTTGAACTAGAGCAAGATAATCTAGTGGTCAAAATGATGTCACGATAGACATCAACCCAAAGAAATAAAAGAACGAATATCATTTTCCAACACCCCTTCCAACTACCGATTTACCTTGCAATTCTAGTAAATATGATAAAGACCTCTTATGATATGAGATTCCC

Coding sequence (CDS)

ATGACATCGACTCTGTCATTTACTCGACCTTTTCTCTTCACTAATGCTTCTAATCTTCTTCGCAGAAACTGCCTCGCAACCTTTTCCTTCTCTGCATCTTTCTCCTCCGACAGCCTTCACCAGAAGAAATGGAGGCAGCCGGTTTCTTCACTTCTTGAGCTTGGCGGAGTCGCTATTGGGAAACATGATGTTGTGAGGGATGATCCCACGAACAATGTCCCAGATAGTATTTTTTCAAAACTTGGTATGCAACTCCACCAAAGAGATAACCATCCACTTGGCATTCTGAAGAATGAGATATACAACTATTTTGATACAAATTACTCAAATAAGTTCACTAAGTTTGATGACCTCAGTCCAATCGTTTCTGTGAAGGAGAATTTTGATGATGTGTTGGTTCCAGAAGATCATGTAAGCCGAAGTTATAATGATACATACTACATTGACTCGCAAACTGTTCTGAGATGTCATACAAGTGCTCACCAAGCGGAGCTGTTGAGAAGAGGCTATACTCAGTTTCTTGTGACAGGCGATGTTTATCGTCGGGATTCCATTGATTCAACTCATTACCCTGTATTTCATCAGATGGAAGGTGTCCGTGTTTTTTCTCTGAATGATTGGGAGGCATCTGGTACAGATGGAACATCTTTTGCAGCTGACGACTTGAAGAAATGTTTGGAAGGTTTGGCAAGGCATTTATTTGGTGCTGTGGAAATGCGCTGGGTTGATACATATTTCCCATTTACAAATCCATCATTTGAACTTGAGATTTTCTTTCAGGAGAAATGGCTGGAAGTATTGGGATGCGGGGTGATGGAGCAAGAAATATTGAAAAGAAGTGATAAAGAAAATAATGTTGCATGGGCTTTTGGTCTCGGGTTGGAAAGGCTAGCTATGGTTCTTTTTGATATACCTGATATCCGATTATTTTGGTCAGCTGACGAGCGATTTACCACGCAGTTCTCAAAGGGCAAGCTAGGTGTCAAGTTTAAATCGTTTTCTAAGTTTCCACCTTGCTACAAGGACATCAGTTTTTGGATCAATGATTCCTTCACCGAGAATAATTTATGTGAAGTTGTTAGAGGAGTTGCCGGCGACCTTGTTGAAGAGGTACAACTGATAGACAGTTTTACCAACAAGAAGAAAGGGATGACGAGTCACTGTTACAGGATTGCTTACCGATCTATGGAACGGTCACTCACAGATGAAGAAATCAATGATTTGCAGTGGAACGTGAGAGAACAGGTTCAGAGCAAGTTGAACGTTGTCTTGCGATGA

Protein sequence

MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIGKHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR
Homology
BLAST of Carg24606 vs. NCBI nr
Match: XP_022926059.1 (phenylalanine--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita moschata] >KAG7034804.1 Phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 874.0 bits (2257), Expect = 5.2e-250
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 60
           MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG
Sbjct: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 60

Query: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120
           KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP
Sbjct: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120

Query: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180
           IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY
Sbjct: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180

Query: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 240
           RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR
Sbjct: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 240

Query: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300
           WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV
Sbjct: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300

Query: 301 LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360
           LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV
Sbjct: 301 LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360

Query: 361 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420
           RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL
Sbjct: 361 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420

Query: 421 NVVLR 426
           NVVLR
Sbjct: 421 NVVLR 425

BLAST of Carg24606 vs. NCBI nr
Match: KAG6581513.1 (Phenylalanine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 870.2 bits (2247), Expect = 7.6e-249
Identity = 423/425 (99.53%), Postives = 424/425 (99.76%), Query Frame = 0

Query: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 60
           MTSTLS TRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG
Sbjct: 40  MTSTLSLTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 99

Query: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120
           KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP
Sbjct: 100 KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 159

Query: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180
           IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY
Sbjct: 160 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 219

Query: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 240
           RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR
Sbjct: 220 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 279

Query: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300
           WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV
Sbjct: 280 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 339

Query: 301 LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360
           LFDIPDIRLFWSADERFT+QFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV
Sbjct: 340 LFDIPDIRLFWSADERFTSQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 399

Query: 361 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420
           RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL
Sbjct: 400 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 459

Query: 421 NVVLR 426
           NVVLR
Sbjct: 460 NVVLR 464

BLAST of Carg24606 vs. NCBI nr
Match: XP_023544877.1 (phenylalanine--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 865.5 bits (2235), Expect = 1.9e-247
Identity = 421/425 (99.06%), Postives = 422/425 (99.29%), Query Frame = 0

Query: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 60
           MTSTLS  RPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG
Sbjct: 1   MTSTLSLARPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 60

Query: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120
           KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP
Sbjct: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120

Query: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180
           IVSVKENFDDVLVP DHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY
Sbjct: 121 IVSVKENFDDVLVPVDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180

Query: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 240
           RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR
Sbjct: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 240

Query: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300
           WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV
Sbjct: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300

Query: 301 LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360
           LFDIPDIRLFWSADERFT+QFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV
Sbjct: 301 LFDIPDIRLFWSADERFTSQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360

Query: 361 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420
           RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL
Sbjct: 361 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420

Query: 421 NVVLR 426
           NVVLR
Sbjct: 421 NVVLR 425

BLAST of Carg24606 vs. NCBI nr
Match: XP_022978338.1 (phenylalanine--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 860.1 bits (2221), Expect = 7.8e-246
Identity = 417/425 (98.12%), Postives = 421/425 (99.06%), Query Frame = 0

Query: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 60
           MTSTLS TRP LFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGV+IG
Sbjct: 1   MTSTLSLTRPLLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVSIG 60

Query: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120
           KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP
Sbjct: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120

Query: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180
           IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY
Sbjct: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180

Query: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 240
           RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTS AADDLKKCLEGLARHLFGAVEMR
Sbjct: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSLAADDLKKCLEGLARHLFGAVEMR 240

Query: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300
           WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV
Sbjct: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300

Query: 301 LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360
           LFDIPDIRLFWSADERFT+QFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV
Sbjct: 301 LFDIPDIRLFWSADERFTSQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360

Query: 361 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420
           RG+AGDLVEEV+LIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL
Sbjct: 361 RGIAGDLVEEVKLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420

Query: 421 NVVLR 426
            VVLR
Sbjct: 421 KVVLR 425

BLAST of Carg24606 vs. NCBI nr
Match: XP_038883400.1 (phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida] >XP_038883401.1 phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida] >XP_038883402.1 phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 805.8 bits (2080), Expect = 1.7e-229
Identity = 392/431 (90.95%), Postives = 408/431 (94.66%), Query Frame = 0

Query: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASF------SSDSLHQKKWRQPVSSLLEL 60
           M STLS T+  LFTNASNLLRRNCL TF FSASF      SSD++H+KKWRQPVSSLLEL
Sbjct: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60

Query: 61  GGVAIGKHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTK 120
           GG+AI K DVVRDDPTNNVPD+IFSKLGMQLH+RDNHPLGILKNEIY YFD NYSNKF K
Sbjct: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120

Query: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180
           FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL
Sbjct: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180

Query: 181 VTGDVYRRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLF 240
           VTGDVYRRDSIDSTHYPVFHQME VRVFSLNDWEASGTDGTS+AADDLKKCLEGLARHLF
Sbjct: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240

Query: 241 GAVEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGL 300
           GAVEMRWVD YFPFTNPSFELEIFFQEKWLEVLGCGVMEQEIL++  KEN+VAWAFGLGL
Sbjct: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300

Query: 301 ERLAMVLFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTEN 360
           ERLAMVLFDIPD+RLFWSADERFT+QFSKGKLGVKFK FSKFPPCYKDISFWINDSFTEN
Sbjct: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360

Query: 361 NLCEVVRGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVRE 420
           NLCEVVRG+AGDLVEEVQLIDSFTN+KKGMTSHCYRIAYRSMERSLTDEEINDLQ NVRE
Sbjct: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420

Query: 421 QVQSKLNVVLR 426
           QVQS+LNVVLR
Sbjct: 421 QVQSRLNVVLR 431

BLAST of Carg24606 vs. ExPASy Swiss-Prot
Match: Q94K73 (Phenylalanine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g58140 PE=1 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 2.6e-191
Identity = 323/420 (76.90%), Postives = 366/420 (87.14%), Query Frame = 0

Query: 12  LFTNAS-NLLRRNCLATFSFSASFSSDSLH----QKKWRQPVSSLLELGGVAIGKHDVVR 71
           +F+ AS  LL  N    FSF +SFSS + +     +K R P+ S +++GGVAI ++DVVR
Sbjct: 10  IFSRASVALLSSNGFKRFSFVSSFSSSAAYSPPKMRKRRYPIVSAVDIGGVAIARNDVVR 69

Query: 72  -DDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVK 131
            DDPTNNVPDSIFSKLGMQLH+RD HP+GILKN IY+YFD+NYSNKF KF+DLSPIV+ K
Sbjct: 70  EDDPTNNVPDSIFSKLGMQLHRRDKHPIGILKNAIYDYFDSNYSNKFEKFEDLSPIVTTK 129

Query: 132 ENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSI 191
           +NFDDVLVP DHVSRS NDTYY+DSQTVLRCHTSAHQAELLR+G+++FLVTGDVYRRDSI
Sbjct: 130 QNFDDVLVPADHVSRSLNDTYYVDSQTVLRCHTSAHQAELLRKGHSRFLVTGDVYRRDSI 189

Query: 192 DSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTY 251
           DSTHYPVFHQMEG  VFS  DW  SG D T +AA+DLKKCLEGLARHLFG+VEMRWVDTY
Sbjct: 190 DSTHYPVFHQMEGFCVFSPEDWNGSGKDSTLYAAEDLKKCLEGLARHLFGSVEMRWVDTY 249

Query: 252 FPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIP 311
           FPFTNPSFELEI+F+E WLEVLGCGV EQ ILK+S  ENNVAWAFGLGLERLAMVLFDIP
Sbjct: 250 FPFTNPSFELEIYFKEDWLEVLGCGVTEQVILKQSGLENNVAWAFGLGLERLAMVLFDIP 309

Query: 312 DIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAG 371
           DIR FWS+DERFT+QF KG+LGVKFK +SK+PPCYKDISFWI+D FTENN CEVVRG+AG
Sbjct: 310 DIRFFWSSDERFTSQFGKGELGVKFKPYSKYPPCYKDISFWISDLFTENNFCEVVRGIAG 369

Query: 372 DLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 426
           DLVEEV+LID FTNKKKG+TSHCYRI +RSMERSLTDEE+NDLQ  VR++VQ KLNV LR
Sbjct: 370 DLVEEVKLIDQFTNKKKGLTSHCYRIVFRSMERSLTDEEVNDLQSKVRDEVQKKLNVELR 429

BLAST of Carg24606 vs. ExPASy Swiss-Prot
Match: Q86A68 (Phenylalanine--tRNA ligase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=mpheS PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 3.3e-106
Identity = 208/419 (49.64%), Postives = 263/419 (62.77%), Query Frame = 0

Query: 60  GKHDVVRDDPTN-NVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYS---NKFTKF 119
           GK D  +  P N N+  SI  K+G  LH + +HPL I+K +I  +F    S   +KF  F
Sbjct: 38  GKIDYTKLIPENGNISKSITEKIGKNLHCKRDHPLNIIKKKIQYHFQNKLSDEEHKFQFF 97

Query: 120 DDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLV 179
           D   P VSVKENFD++L P DHV RS NDTYY     +LR HTSAHQ++LLR     FLV
Sbjct: 98  DSFEPKVSVKENFDELLFPVDHVGRSPNDTYYFSKDQLLRTHTSAHQSQLLREQEKAFLV 157

Query: 180 TGDVYRRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSF------------------ 239
           TGDVYRRD+ID+ HYPVFHQMEGV+VF   D      DG  F                  
Sbjct: 158 TGDVYRRDTIDAVHYPVFHQMEGVKVF--KDKVNLAIDGKPFDETIDYYEDNNYKQLQSV 217

Query: 240 --AADDLKKCLEGLARHLFGA-VEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQ 299
                DLK+ LE + R + G  +++RW+D YFPFT+PSFE+EI+FQ +WLEVLGCGV+  
Sbjct: 218 KDVEKDLKQSLESMIRSVIGQDLQVRWIDAYFPFTSPSFEMEIYFQGQWLEVLGCGVVHP 277

Query: 300 EILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQF------------- 359
            I+      N+ AWAFG+GLERLAM+LF+IPDIRLFW+ D RF  QF             
Sbjct: 278 SIMNNCGLSNDRAWAFGIGLERLAMILFNIPDIRLFWTEDNRFHNQFKGVDKSISTSSSS 337

Query: 360 -------SKGKL------GVKFKSFSKFPPCYKDISFWIND--SFTENNLCEVVRGVAGD 419
                  S   L      GV+F+ FSK+P C+KD+SFW+ D  +F EN   E VR   GD
Sbjct: 338 SSSSSSSSSSTLSDIDIKGVQFQQFSKYPSCFKDVSFWLEDEENFHENKFYEFVRESCGD 397

Query: 420 LVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 426
           LVE V L+D+FTNKK   TSHCYRI YRSM+R+LT+EEI+ LQ+N+RE++++ L+V LR
Sbjct: 398 LVERVDLVDNFTNKKLNKTSHCYRIYYRSMDRNLTNEEIDILQFNLREKLENHLSVKLR 454

BLAST of Carg24606 vs. ExPASy Swiss-Prot
Match: Q99M01 (Phenylalanine--tRNA ligase, mitochondrial OS=Mus musculus OX=10090 GN=Fars2 PE=1 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 8.8e-99
Identity = 187/389 (48.07%), Postives = 246/389 (63.24%), Query Frame = 0

Query: 66  RDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNK-----FTKFDDLSP 125
           +DD T N+   + SK+G  LH +  HPL ++K  +  +F   Y  +     F+ +D L P
Sbjct: 60  QDDHT-NLTQKVLSKVGRNLHNQKFHPLWLIKERVKEHFYQQYMVRSRTPLFSVYDQLPP 119

Query: 126 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 185
           +V+  +NFD +L+P DH SR   D YY++   +LR HTSAHQ +LL  G   FLV GDVY
Sbjct: 120 VVTTWQNFDSLLIPADHPSRKKGDNYYLNRAHMLRAHTSAHQWDLLHAGLNAFLVVGDVY 179

Query: 186 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTS-----------------------F 245
           RRD ID  HYPVFHQ+EGVR+FS ++  A   DG S                        
Sbjct: 180 RRDQIDCQHYPVFHQLEGVRLFSKHELFAGVKDGESLQLFEEGSRSAHKQETHTMEAVKL 239

Query: 246 AADDLKKCLEGLARHLFG-AVEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEI 305
              DLK+ L  L  HLFG  +E+RWVD YFPFT+PSFE+EI F+ +WLEVLGCGVMEQ++
Sbjct: 240 VEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFEMEINFRGEWLEVLGCGVMEQQL 299

Query: 306 LKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQF--SKGKLGVKFKSFS 365
           +  +  ++ + WAFGLGLERLAMVL+DIPDIRLFWS DERF  QF  S     VKF+  S
Sbjct: 300 VNSAGAQDRIGWAFGLGLERLAMVLYDIPDIRLFWSEDERFLKQFLLSDINQSVKFQPLS 359

Query: 366 KFPPCYKDISFWI-NDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAY 423
           K+P  + DISFW+ ++++TEN+  ++VR V GDLVE+V LID F + K   TSHCYRI Y
Sbjct: 360 KYPAVFNDISFWLPSENYTENDFYDIVRTVGGDLVEKVDLIDKFEHPKTHRTSHCYRITY 419

BLAST of Carg24606 vs. ExPASy Swiss-Prot
Match: Q6AYQ3 (Phenylalanine--tRNA ligase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fars2 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.3e-97
Identity = 188/410 (45.85%), Postives = 249/410 (60.73%), Query Frame = 0

Query: 66  RDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNK-----FTKFDDLSP 125
           +DD T N+   + SK+G  LH +  HPL ++K  +  +F   Y  +     F+ +D LSP
Sbjct: 60  QDDHT-NLTQKVLSKVGRNLHNQKFHPLWLIKERVKEHFYQQYMGRSRTPLFSVYDQLSP 119

Query: 126 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 185
           +V+  +NFD +L+P DH SR   D YY++   +LR HTSAHQ +LL  G   FLV GDVY
Sbjct: 120 VVTTWQNFDSLLIPADHPSRKKGDNYYLNRGHMLRAHTSAHQWDLLHAGLNAFLVVGDVY 179

Query: 186 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTS-----------------------F 245
           RRD IDS HYPVFHQ+EGVR+FS ++  A   DG S                        
Sbjct: 180 RRDQIDSQHYPVFHQLEGVRLFSKHELFAGVKDGESLQLFEESSRSAHKQETHTMEAVKL 239

Query: 246 AADDLKKCLEGLARHLFG-AVEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEI 305
              DLK+ L  L  HLFG  +E+RWVD YFPFT+PSFE+EI F+ +WLEVLGCGVMEQ++
Sbjct: 240 VEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFEMEINFRGEWLEVLGCGVMEQQL 299

Query: 306 LKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS-------------- 365
           +  +  ++ + WAFGLGLERLAMVL+DIPDIRLFWS DERF  QF               
Sbjct: 300 VNSAGAQDRIGWAFGLGLERLAMVLYDIPDIRLFWSEDERFLKQFRLSDINQSVKFQRWF 359

Query: 366 ---------KGKLGVKFKSFSKFPPCYKDISFWI-NDSFTENNLCEVVRGVAGDLVEEVQ 423
                    + K+G +   FSK+P  + DISFW+ ++++TEN+  ++VR V GDLVE+V 
Sbjct: 360 FQEERATGIQRKMGRQLCPFSKYPAVFNDISFWLPSENYTENDFYDIVRTVGGDLVEKVD 419

BLAST of Carg24606 vs. ExPASy Swiss-Prot
Match: O95363 (Phenylalanine--tRNA ligase, mitochondrial OS=Homo sapiens OX=9606 GN=FARS2 PE=1 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.1e-96
Identity = 177/387 (45.74%), Postives = 242/387 (62.53%), Query Frame = 0

Query: 68  DPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNK-----FTKFDDLSPIV 127
           D  +N+   + +++G  LH + +HPL ++K  +  +F   Y  +     F+ +D+LSP+V
Sbjct: 61  DDHSNLTRKVLTRVGRNLHNQQHHPLWLIKERVKEHFYKQYVGRFGTPLFSVYDNLSPVV 120

Query: 128 SVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRR 187
           +  +NFD +L+P DH SR   D YY++   +LR HTSAHQ +LL  G   FLV GDVYRR
Sbjct: 121 TTWQNFDSLLIPADHPSRKKGDNYYLNRTHMLRAHTSAHQWDLLHAGLDAFLVVGDVYRR 180

Query: 188 DSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTS-----------------------FAA 247
           D IDS HYP+FHQ+E VR+FS ++  A   DG S                          
Sbjct: 181 DQIDSQHYPIFHQLEAVRLFSKHELFAGIKDGESLQLFEQSSRSAHKQETHTMEAVKLVE 240

Query: 248 DDLKKCLEGLARHLFG-AVEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILK 307
            DLK+ L  L  HLFG  +E+RWVD YFPFT+PSFE+EI F  +WLEVLGCGVMEQ+++ 
Sbjct: 241 FDLKQTLTRLMAHLFGDELEIRWVDCYFPFTHPSFEMEINFHGEWLEVLGCGVMEQQLVN 300

Query: 308 RSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFSKGKLG--VKFKSFSKF 367
            +  ++ + WAFGLGLERLAM+L+DIPDIRLFW  DERF  QF    +   VKF+  SK+
Sbjct: 301 SAGAQDRIGWAFGLGLERLAMILYDIPDIRLFWCEDERFLKQFCVSNINQKVKFQPLSKY 360

Query: 368 PPCYKDISFWI-NDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRS 423
           P    DISFW+ ++++ EN+  ++VR + GDLVE+V LID F + K   TSHCYRI YR 
Sbjct: 361 PAVINDISFWLPSENYAENDFYDLVRTIGGDLVEKVDLIDKFVHPKTHKTSHCYRITYRH 420

BLAST of Carg24606 vs. ExPASy TrEMBL
Match: A0A6J1EDY7 (Phenylalanyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433292 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.5e-250
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 60
           MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG
Sbjct: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 60

Query: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120
           KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP
Sbjct: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120

Query: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180
           IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY
Sbjct: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180

Query: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 240
           RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR
Sbjct: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 240

Query: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300
           WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV
Sbjct: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300

Query: 301 LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360
           LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV
Sbjct: 301 LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360

Query: 361 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420
           RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL
Sbjct: 361 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420

Query: 421 NVVLR 426
           NVVLR
Sbjct: 421 NVVLR 425

BLAST of Carg24606 vs. ExPASy TrEMBL
Match: A0A6J1IST7 (Phenylalanyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111478364 PE=3 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 3.8e-246
Identity = 417/425 (98.12%), Postives = 421/425 (99.06%), Query Frame = 0

Query: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 60
           MTSTLS TRP LFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGV+IG
Sbjct: 1   MTSTLSLTRPLLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVSIG 60

Query: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120
           KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP
Sbjct: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120

Query: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180
           IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY
Sbjct: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180

Query: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 240
           RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTS AADDLKKCLEGLARHLFGAVEMR
Sbjct: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSLAADDLKKCLEGLARHLFGAVEMR 240

Query: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300
           WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV
Sbjct: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300

Query: 301 LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360
           LFDIPDIRLFWSADERFT+QFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV
Sbjct: 301 LFDIPDIRLFWSADERFTSQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360

Query: 361 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420
           RG+AGDLVEEV+LIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL
Sbjct: 361 RGIAGDLVEEVKLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420

Query: 421 NVVLR 426
            VVLR
Sbjct: 421 KVVLR 425

BLAST of Carg24606 vs. ExPASy TrEMBL
Match: A0A6J1KMS1 (Phenylalanyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111497099 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 1.9e-229
Identity = 388/425 (91.29%), Postives = 404/425 (95.06%), Query Frame = 0

Query: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 60
           M STL  TR FLF +ASN LRRNCL +F+FSA+FSSDS+H KKWR PVSSL ELGGVAI 
Sbjct: 1   MASTLPLTRAFLFADASNHLRRNCLRSFAFSAAFSSDSVHCKKWRHPVSSLPELGGVAIA 60

Query: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120
           + DVVRDDPTNNVPD+IFSKLG+QLH++DNHPLGILKNEIY YFDTNYSNKF KFDDLSP
Sbjct: 61  RDDVVRDDPTNNVPDNIFSKLGVQLHRKDNHPLGILKNEIYKYFDTNYSNKFDKFDDLSP 120

Query: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180
           IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLR GYTQFLVTGDVY
Sbjct: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRSGYTQFLVTGDVY 180

Query: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 240
           RRDSIDSTHYPVFHQMEGVRVFSLNDWEAS TDGTS+AADDLKKCLEGLARHLFGAVEMR
Sbjct: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASSTDGTSYAADDLKKCLEGLARHLFGAVEMR 240

Query: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300
           WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRS KENNVAWAFGLGLERLAMV
Sbjct: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSGKENNVAWAFGLGLERLAMV 300

Query: 301 LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360
           LFDIPDIRLFWSADERFT+QFSKGKLGVKFK FSKFPPCYKD+SFWIN+SFTENNLCEVV
Sbjct: 301 LFDIPDIRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDVSFWINESFTENNLCEVV 360

Query: 361 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420
           RG+AGDLVEEV+LIDSFTN KKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQV SKL
Sbjct: 361 RGIAGDLVEEVRLIDSFTNNKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVHSKL 420

Query: 421 NVVLR 426
            VVLR
Sbjct: 421 KVVLR 425

BLAST of Carg24606 vs. ExPASy TrEMBL
Match: A0A6J1EEM6 (Phenylalanyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433642 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 9.4e-229
Identity = 386/425 (90.82%), Postives = 405/425 (95.29%), Query Frame = 0

Query: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASFSSDSLHQKKWRQPVSSLLELGGVAIG 60
           M STL  TR FLF +AS+ LRRNCL +F+FSA+FSSDS+H KKWR PVSSL ELGGVAI 
Sbjct: 1   MASTLPLTRAFLFADASSHLRRNCLRSFAFSAAFSSDSVHCKKWRHPVSSLSELGGVAIA 60

Query: 61  KHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSP 120
           + DVVRDDPTNNVPD+IFSKLG+QLH++DNHPLGILKNEIY+YFDTNYSNKF KFDDLSP
Sbjct: 61  RDDVVRDDPTNNVPDNIFSKLGVQLHRKDNHPLGILKNEIYDYFDTNYSNKFDKFDDLSP 120

Query: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVY 180
           IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLR GYTQFLVTGDVY
Sbjct: 121 IVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRSGYTQFLVTGDVY 180

Query: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMR 240
           RRDSIDSTHYPVFHQMEGVRVFSLNDWEAS TDGTS+AADDLKKCLEGLARHLFGAVEMR
Sbjct: 181 RRDSIDSTHYPVFHQMEGVRVFSLNDWEASSTDGTSYAADDLKKCLEGLARHLFGAVEMR 240

Query: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMV 300
           WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRS KENNVAWAFGLGLERLAMV
Sbjct: 241 WVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSGKENNVAWAFGLGLERLAMV 300

Query: 301 LFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVV 360
           LFDIPDIRLFWSADERFT+QFSKGKLGVKFK FSKFPPCYKD+SFWIN+SFTENNLCEVV
Sbjct: 301 LFDIPDIRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDVSFWINESFTENNLCEVV 360

Query: 361 RGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKL 420
           RG+AGDLVEEV+LIDS+TN KKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQV SKL
Sbjct: 361 RGIAGDLVEEVRLIDSYTNNKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVHSKL 420

Query: 421 NVVLR 426
            VVLR
Sbjct: 421 KVVLR 425

BLAST of Carg24606 vs. ExPASy TrEMBL
Match: A0A6J1CZ26 (Phenylalanyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111015788 PE=3 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 1.8e-227
Identity = 390/426 (91.55%), Postives = 405/426 (95.07%), Query Frame = 0

Query: 1   MTSTLSFTRPFLFTNASNLLRRNCLATFSFSASF-SSDSLHQKKWRQPVSSLLELGGVAI 60
           M STL  TR  LFTNAS LLRRNCL  F+FSASF SSD++H+KKWRQPVSSLL+LGGV I
Sbjct: 1   MASTLPLTRAILFTNASILLRRNCLRNFAFSASFSSSDTVHRKKWRQPVSSLLQLGGVVI 60

Query: 61  GKHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLS 120
            K DVVRDDPTNNVPD+IFSKLGMQLH+RDNHPLGILKNEIYNYFDTNYSNKF KFDDLS
Sbjct: 61  AKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYNYFDTNYSNKFDKFDDLS 120

Query: 121 PIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDV 180
           PIVSVKENFDDVLVP DHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRG TQFLVTGDV
Sbjct: 121 PIVSVKENFDDVLVPADHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGNTQFLVTGDV 180

Query: 181 YRRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEM 240
           YRRDSIDSTHYPVFHQMEGVRV S++DWEASGTDGTS+AA+DLKKCLEGLARHLFGAVEM
Sbjct: 181 YRRDSIDSTHYPVFHQMEGVRVVSVDDWEASGTDGTSYAANDLKKCLEGLARHLFGAVEM 240

Query: 241 RWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAM 300
           RWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEIL RS K NNVAWAFGLGLERLAM
Sbjct: 241 RWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILTRSGKTNNVAWAFGLGLERLAM 300

Query: 301 VLFDIPDIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEV 360
           VLFDIPDIRLFWSADERFT+QFSKGKLGVKFK FSKFPPCYKDISFWIN+SFTENNLCEV
Sbjct: 301 VLFDIPDIRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINESFTENNLCEV 360

Query: 361 VRGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSK 420
           VRG+AGDLVEEVQLID+FTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQ NVREQVQSK
Sbjct: 361 VRGIAGDLVEEVQLIDNFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVREQVQSK 420

Query: 421 LNVVLR 426
           LNVVLR
Sbjct: 421 LNVVLR 426

BLAST of Carg24606 vs. TAIR 10
Match: AT3G58140.1 (phenylalanyl-tRNA synthetase class IIc family protein )

HSP 1 Score: 669.5 bits (1726), Expect = 1.8e-192
Identity = 323/420 (76.90%), Postives = 366/420 (87.14%), Query Frame = 0

Query: 12  LFTNAS-NLLRRNCLATFSFSASFSSDSLH----QKKWRQPVSSLLELGGVAIGKHDVVR 71
           +F+ AS  LL  N    FSF +SFSS + +     +K R P+ S +++GGVAI ++DVVR
Sbjct: 10  IFSRASVALLSSNGFKRFSFVSSFSSSAAYSPPKMRKRRYPIVSAVDIGGVAIARNDVVR 69

Query: 72  -DDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVK 131
            DDPTNNVPDSIFSKLGMQLH+RD HP+GILKN IY+YFD+NYSNKF KF+DLSPIV+ K
Sbjct: 70  EDDPTNNVPDSIFSKLGMQLHRRDKHPIGILKNAIYDYFDSNYSNKFEKFEDLSPIVTTK 129

Query: 132 ENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSI 191
           +NFDDVLVP DHVSRS NDTYY+DSQTVLRCHTSAHQAELLR+G+++FLVTGDVYRRDSI
Sbjct: 130 QNFDDVLVPADHVSRSLNDTYYVDSQTVLRCHTSAHQAELLRKGHSRFLVTGDVYRRDSI 189

Query: 192 DSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTY 251
           DSTHYPVFHQMEG  VFS  DW  SG D T +AA+DLKKCLEGLARHLFG+VEMRWVDTY
Sbjct: 190 DSTHYPVFHQMEGFCVFSPEDWNGSGKDSTLYAAEDLKKCLEGLARHLFGSVEMRWVDTY 249

Query: 252 FPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIP 311
           FPFTNPSFELEI+F+E WLEVLGCGV EQ ILK+S  ENNVAWAFGLGLERLAMVLFDIP
Sbjct: 250 FPFTNPSFELEIYFKEDWLEVLGCGVTEQVILKQSGLENNVAWAFGLGLERLAMVLFDIP 309

Query: 312 DIRLFWSADERFTTQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAG 371
           DIR FWS+DERFT+QF KG+LGVKFK +SK+PPCYKDISFWI+D FTENN CEVVRG+AG
Sbjct: 310 DIRFFWSSDERFTSQFGKGELGVKFKPYSKYPPCYKDISFWISDLFTENNFCEVVRGIAG 369

Query: 372 DLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 426
           DLVEEV+LID FTNKKKG+TSHCYRI +RSMERSLTDEE+NDLQ  VR++VQ KLNV LR
Sbjct: 370 DLVEEVKLIDQFTNKKKGLTSHCYRIVFRSMERSLTDEEVNDLQSKVRDEVQKKLNVELR 429

BLAST of Carg24606 vs. TAIR 10
Match: AT4G39280.1 (phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative )

HSP 1 Score: 65.5 bits (158), Expect = 1.2e-10
Identity = 55/200 (27.50%), Postives = 92/200 (46.00%), Query Frame = 0

Query: 133 VPEDHV-------------SRSYNDTYYID--SQTVLRCHTSAHQAELL----RRGYT-- 192
           +PED+V             SR YN  +  +  ++ +LR HT+A  + +L    ++ +   
Sbjct: 277 LPEDYVERVKRVHESGGYGSRGYNYDWKREEANKNLLRTHTTAVSSRMLYALAQKPFVPK 336

Query: 193 QFLVTGDVYRRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLAR 252
           ++     V+R +++D TH   FHQ+EG+                     DL   LE    
Sbjct: 337 KYFSIDRVFRNEAVDRTHLAEFHQIEGL------------ICDRGLTLGDLIGVLEDFFS 396

Query: 253 HLFGAVEMRWVDTYFPFTNPSFELEIFFQ--EKWLEVLGCGVMEQEILKRSDKENNV-AW 309
            L G  ++R+   Y P+T PS E+  + +   KW+E+   G+   E+L       +V   
Sbjct: 397 RL-GMSKLRFKPAYNPYTEPSMEIFSYHEGLGKWVEIGNSGMFRPEMLLPMGLPEDVRVI 456

BLAST of Carg24606 vs. TAIR 10
Match: AT4G39280.2 (phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative )

HSP 1 Score: 65.5 bits (158), Expect = 1.2e-10
Identity = 55/200 (27.50%), Postives = 92/200 (46.00%), Query Frame = 0

Query: 133 VPEDHV-------------SRSYNDTYYID--SQTVLRCHTSAHQAELL----RRGYT-- 192
           +PED+V             SR YN  +  +  ++ +LR HT+A  + +L    ++ +   
Sbjct: 277 LPEDYVERVKRVHESGGYGSRGYNYDWKREEANKNLLRTHTTAVSSRMLYALAQKPFVPK 336

Query: 193 QFLVTGDVYRRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLAR 252
           ++     V+R +++D TH   FHQ+EG+                     DL   LE    
Sbjct: 337 KYFSIDRVFRNEAVDRTHLAEFHQIEGL------------ICDRGLTLGDLIGVLEDFFS 396

Query: 253 HLFGAVEMRWVDTYFPFTNPSFELEIFFQ--EKWLEVLGCGVMEQEILKRSDKENNV-AW 309
            L G  ++R+   Y P+T PS E+  + +   KW+E+   G+   E+L       +V   
Sbjct: 397 RL-GMSKLRFKPAYNPYTEPSMEIFSYHEGLGKWVEIGNSGMFRPEMLLPMGLPEDVRVI 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022926059.15.2e-250100.00phenylalanine--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbi... [more]
KAG6581513.17.6e-24999.53Phenylalanine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argy... [more]
XP_023544877.11.9e-24799.06phenylalanine--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbi... [more]
XP_022978338.17.8e-24698.12phenylalanine--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbi... [more]
XP_038883400.11.7e-22990.95phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida] >XP_... [more]
Match NameE-valueIdentityDescription
Q94K732.6e-19176.90Phenylalanine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana ... [more]
Q86A683.3e-10649.64Phenylalanine--tRNA ligase, mitochondrial OS=Dictyostelium discoideum OX=44689 G... [more]
Q99M018.8e-9948.07Phenylalanine--tRNA ligase, mitochondrial OS=Mus musculus OX=10090 GN=Fars2 PE=1... [more]
Q6AYQ31.3e-9745.85Phenylalanine--tRNA ligase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fars2... [more]
O953633.1e-9645.74Phenylalanine--tRNA ligase, mitochondrial OS=Homo sapiens OX=9606 GN=FARS2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1EDY72.5e-250100.00Phenylalanyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433292 PE=3 ... [more]
A0A6J1IST73.8e-24698.12Phenylalanyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111478364 PE=3 SV... [more]
A0A6J1KMS11.9e-22991.29Phenylalanyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111497099 PE=3 SV... [more]
A0A6J1EEM69.4e-22990.82Phenylalanyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433642 PE=3 ... [more]
A0A6J1CZ261.8e-22791.55Phenylalanyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111015788 PE=3... [more]
Match NameE-valueIdentityDescription
AT3G58140.11.8e-19276.90phenylalanyl-tRNA synthetase class IIc family protein [more]
AT4G39280.11.2e-1027.50phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative [more]
AT4G39280.21.2e-1027.50phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005121Ferrodoxin-fold anticodon-binding domainSMARTSM00896FDX_ACB_2coord: 334..425
e-value: 1.9E-33
score: 127.1
IPR005121Ferrodoxin-fold anticodon-binding domainPFAMPF03147FDX-ACBcoord: 334..425
e-value: 1.3E-26
score: 92.9
IPR005121Ferrodoxin-fold anticodon-binding domainPROSITEPS51447FDX_ACBcoord: 334..425
score: 25.747673
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 58..324
e-value: 2.1E-97
score: 328.2
NoneNo IPR availablePANTHERPTHR11538PHENYLALANYL-TRNA SYNTHETASEcoord: 27..420
NoneNo IPR availableCDDcd00496PheRS_alpha_corecoord: 91..316
e-value: 1.07078E-96
score: 287.134
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 81..321
IPR036690Ferrodoxin-fold anticodon-binding domain superfamilyGENE3D3.30.70.380coord: 330..424
e-value: 3.3E-34
score: 118.7
IPR036690Ferrodoxin-fold anticodon-binding domain superfamilySUPERFAMILY54991Anticodon-binding domain of PheRScoord: 329..425
IPR002319Phenylalanyl-tRNA synthetasePFAMPF01409tRNA-synt_2dcoord: 84..321
e-value: 2.5E-50
score: 171.3
IPR004530Phenylalanyl-tRNA synthetase, class IIc, mitochondrialTIGRFAMTIGR00469TIGR00469coord: 236..422
e-value: 4.4E-78
score: 260.9
IPR004530Phenylalanyl-tRNA synthetase, class IIc, mitochondrialPANTHERPTHR11538:SF41PHENYLALANINE--TRNA LIGASE, MITOCHONDRIALcoord: 27..420
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 177..338
score: 12.137974

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24606-RACarg24606-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006432 phenylalanyl-tRNA aminoacylation
biological_process GO:0043039 tRNA aminoacylation
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004826 phenylalanine-tRNA ligase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding