Carg24569 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24569
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	chaperone protein dnaJ 20, chloroplastic-like
Location	Carg_Chr19: 2109522 .. 2109965 (-)
RNA-Seq Expression	Carg24569
Synteny	Carg24569

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGGCACTCCGACCAGGCCGATGGTGGGCTTGTCCTGCAAGGCTGCTACCACCAAAGGGCCTGATTATTACAAATTGCTGTCGGTGAGTAGCGGCTGCAATGCAAGCGGTGAAGAGATCAAGAGGGCTTATAGAGCCATGGCTTTGCAGTATCATCCTGACCTTGTTTGTGATCCTTTGCTCAAAGAGCAGTCTACCAAGATGTTTGTTCAGCTGAATGCGGCGTACAAGACGCTCTCTGATCCTGTTCTTAGAAGACAATATGATGACTCTATGATGGGTTTGAATAATGGGTATTCCTATGAGAGAGGAGGCTTTGAGGTGGATAGAACCGTGTGGCAAAGGCAGATTCTTGAGCTCAAGCGTCGGTCTGCATGTCAAAGGGATCGGTCGGTGTCGTGGGGTGGGAGAATGCGGGCTCGGAATTACTCGGAATGTTAA

mRNA sequence

ATGGTGGGCACTCCGACCAGGCCGATGGTGGGCTTGTCCTGCAAGGCTGCTACCACCAAAGGGCCTGATTATTACAAATTGCTGTCGGTGAGTAGCGGCTGCAATGCAAGCGGTGAAGAGATCAAGAGGGCTTATAGAGCCATGGCTTTGCAGTATCATCCTGACCTTGTTTGTGATCCTTTGCTCAAAGAGCAGTCTACCAAGATGTTTGTTCAGCTGAATGCGGCGTACAAGACGCTCTCTGATCCTGTTCTTAGAAGACAATATGATGACTCTATGATGGGTTTGAATAATGGGTATTCCTATGAGAGAGGAGGCTTTGAGGTGGATAGAACCGTGTGGCAAAGGCAGATTCTTGAGCTCAAGCGTCGGTCTGCATGTCAAAGGGATCGGTCGGTGTCGTGGGGTGGGAGAATGCGGGCTCGGAATTACTCGGAATGTTAA

Coding sequence (CDS)

ATGGTGGGCACTCCGACCAGGCCGATGGTGGGCTTGTCCTGCAAGGCTGCTACCACCAAAGGGCCTGATTATTACAAATTGCTGTCGGTGAGTAGCGGCTGCAATGCAAGCGGTGAAGAGATCAAGAGGGCTTATAGAGCCATGGCTTTGCAGTATCATCCTGACCTTGTTTGTGATCCTTTGCTCAAAGAGCAGTCTACCAAGATGTTTGTTCAGCTGAATGCGGCGTACAAGACGCTCTCTGATCCTGTTCTTAGAAGACAATATGATGACTCTATGATGGGTTTGAATAATGGGTATTCCTATGAGAGAGGAGGCTTTGAGGTGGATAGAACCGTGTGGCAAAGGCAGATTCTTGAGCTCAAGCGTCGGTCTGCATGTCAAAGGGATCGGTCGGTGTCGTGGGGTGGGAGAATGCGGGCTCGGAATTACTCGGAATGTTAA

Protein sequence

MVGTPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILELKRRSACQRDRSVSWGGRMRARNYSEC

Homology

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BLAST of Carg24569 vs. NCBI nr
Match: XP_022963776.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata] >XP_023511548.1 chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG6571688.1 Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011414.1 Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 304.3 bits (778), Expect = 5.7e-79
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MVGTPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP 60
           MVGTPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP
Sbjct: 1   MVGTPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP 60

Query: 61  LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILE 120
           LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILE
Sbjct: 61  LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILE 120

Query: 121 LKRRSACQRDRSVSWGGRMRARNYSEC 148
           LKRRSACQRDRSVSWGGRMRARNYSEC
Sbjct: 121 LKRRSACQRDRSVSWGGRMRARNYSEC 147

[Close]

BLAST of Carg24569 vs. NCBI nr
Match: XP_022967659.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 297.0 bits (759), Expect = 9.1e-77
Identity = 144/147 (97.96%), Postives = 144/147 (97.96%), Query Frame = 0


Query: 1   MVGTPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP 60
           MV  PTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP
Sbjct: 1   MVSNPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP 60

Query: 61  LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILE 120
           LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYER GFEVDRTVWQRQILE
Sbjct: 61  LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERRGFEVDRTVWQRQILE 120

Query: 121 LKRRSACQRDRSVSWGGRMRARNYSEC 148
           LKRRSACQRDRSVSWGGRMRARNYSEC
Sbjct: 121 LKRRSACQRDRSVSWGGRMRARNYSEC 147

[Close]

BLAST of Carg24569 vs. NCBI nr
Match: XP_038888723.1 (dnaJ homolog subfamily A member 3, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 201.1 bits (510), Expect = 6.8e-48
Identity = 104/139 (74.82%), Postives = 115/139 (82.73%), Query Frame = 0


Query: 13  SCKAATT----KGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTK 72
           SCKAA      K  DYYKLLSVS GCNAS EEIK+AYRAMALQYHPDLVCDPLLKEQST+
Sbjct: 35  SCKAAAVAAAPKERDYYKLLSVSGGCNASNEEIKKAYRAMALQYHPDLVCDPLLKEQSTR 94

Query: 73  MFVQLNAAYKTLSDPVLRRQYDDSMMGL-NNGYSYERGGFEVDRTVWQRQILELKRRSAC 132
           +FVQLNAAYKTLSDPVLRRQYD S+MG  NNG+S  R GF+ D TVWQRQ+LELKRRSA 
Sbjct: 95  IFVQLNAAYKTLSDPVLRRQYDASLMGFNNNGFSNGRRGFQADTTVWQRQLLELKRRSAL 154

Query: 133 QRDRS--VSWGGRMRARNY 145
           +RDRS   SWG  M+AR++
Sbjct: 155 RRDRSAKASWGATMQARSF 173

[Close]

BLAST of Carg24569 vs. NCBI nr
Match: KAE8645798.1 (hypothetical protein Csa_017276 [Cucumis sativus])

HSP 1 Score: 185.7 bits (470), Expect = 3.0e-43
Identity = 98/138 (71.01%), Postives = 113/138 (81.88%), Query Frame = 0


Query: 12  LSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFV 71
           +SCKA+ T+  DYYKLLSVS GCNAS EEIK+AYRAMAL+YHPDLVCDPLLKEQST+MFV
Sbjct: 23  VSCKASATRERDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFV 82

Query: 72  QLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILELKRRSACQRDR 131
           QLNAAYKTLSDPVLRRQYDDS   LN G++ +  GF  D  VW+RQILELKRRS+ ++DR
Sbjct: 83  QLNAAYKTLSDPVLRRQYDDS---LNMGFNTK--GFRGDSAVWERQILELKRRSSQRKDR 142

Query: 132 S--VSWGGRMRARNYSEC 148
           S   SW  RM+A  +S C
Sbjct: 143 SAPASWAARMQA--HSRC 153

[Close]

BLAST of Carg24569 vs. NCBI nr
Match: XP_004136866.1 (chaperone protein dnaJ 20, chloroplastic [Cucumis sativus])

HSP 1 Score: 185.7 bits (470), Expect = 3.0e-43
Identity = 98/138 (71.01%), Postives = 113/138 (81.88%), Query Frame = 0


Query: 12  LSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFV 71
           +SCKA+ T+  DYYKLLSVS GCNAS EEIK+AYRAMAL+YHPDLVCDPLLKEQST+MFV
Sbjct: 35  VSCKASATRERDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFV 94

Query: 72  QLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILELKRRSACQRDR 131
           QLNAAYKTLSDPVLRRQYDDS   LN G++ +  GF  D  VW+RQILELKRRS+ ++DR
Sbjct: 95  QLNAAYKTLSDPVLRRQYDDS---LNMGFNTK--GFRGDSAVWERQILELKRRSSQRKDR 154

Query: 132 S--VSWGGRMRARNYSEC 148
           S   SW  RM+A  +S C
Sbjct: 155 SAPASWAARMQA--HSRC 165

[Close]

BLAST of Carg24569 vs. ExPASy Swiss-Prot
Match: Q9SDN0 (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 4.1e-11
Identity = 47/127 (37.01%), Postives = 68/127 (53.54%), Query Frame = 0


Query: 24  YYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFVQLNAAYKTLSDP 83
           +Y LL V+   + +  EIK+AY+ +A +YHPD V  P   E+ T  F+++  AY+TLSDP
Sbjct: 67  FYDLLGVTE--SVTLPEIKQAYKQLARKYHPD-VSPPDRVEEYTDRFIRVQEAYETLSDP 126

Query: 84  VLRRQYD-DSMMGLNNGYSYERGGFEVDRTV---------WQRQILELKRRSACQRDRSV 141
             R  YD D  MG +  +S  R        V         WQ Q+  L+RRS  + + ++
Sbjct: 127 RRRVLYDRDLSMGFSFSFSGRRQNRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKDNNTM 186

[Close]

BLAST of Carg24569 vs. ExPASy Swiss-Prot
Match: A4XKA5 (Chaperone protein DnaJ OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331) OX=351627 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 6.9e-11
Identity = 41/86 (47.67%), Postives = 52/86 (60.47%), Query Frame = 0


Query: 23  DYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFVQLNAAYKTLSD 82
           DYY++L V    NA+ EEIKRAYR +A QYHPD   +P  KE   K F ++N AY+ LSD
Sbjct: 6   DYYEILGVPR--NATQEEIKRAYRRLAKQYHPD--ANPGNKEAEEK-FKEINEAYEVLSD 65

Query: 83  PVLRRQYDD-SMMGLNNGYSYERGGF 108
           P  RR+YD       +  Y  + GGF
Sbjct: 66  PEKRRKYDQFGHAAFDPTYGAQGGGF 86

[Close]

BLAST of Carg24569 vs. ExPASy Swiss-Prot
Match: B5YAR4 (Chaperone protein DnaJ OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) OX=309799 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 6.9e-11
Identity = 39/87 (44.83%), Postives = 54/87 (62.07%), Query Frame = 0


Query: 23  DYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFVQLNAAYKTLSD 82
           DYY++L V    NA+ +EIK+AYR +  QYHPDL  DP   E+    F ++N AY+ LSD
Sbjct: 6   DYYEILGVPR--NATQDEIKQAYRRLVRQYHPDLNKDPSAHEK----FKEINEAYEVLSD 65

Query: 83  PVLRRQYDD-SMMGLNNGYSYERGGFE 109
           P  R QYD    +G  +GY   +GG++
Sbjct: 66  PQKRAQYDQFGHVGDFSGYGDFQGGWQ 86

[Close]

BLAST of Carg24569 vs. ExPASy Swiss-Prot
Match: Q9NVH1 (DnaJ homolog subfamily C member 11 OS=Homo sapiens OX=9606 GN=DNAJC11 PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 7.7e-10
Identity = 33/68 (48.53%), Postives = 43/68 (63.24%), Query Frame = 0


Query: 23 DYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFVQLNAAYKTLSD 82
          DYY LL+V     AS EE+K AYR + + YHPD   DP LK Q+ ++F  ++ AY+ LSD
Sbjct: 14 DYYSLLNVRR--EASSEELKAAYRRLCMLYHPDKHRDPELKSQAERLFNLVHQAYEVLSD 73

Query: 83 PVLRRQYD 91
          P  R  YD
Sbjct: 74 PQTRAIYD 79

[Close]

BLAST of Carg24569 vs. ExPASy Swiss-Prot
Match: Q5U458 (DnaJ homolog subfamily C member 11 OS=Mus musculus OX=10090 GN=Dnajc11 PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 7.7e-10
Identity = 33/68 (48.53%), Postives = 43/68 (63.24%), Query Frame = 0


Query: 23 DYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFVQLNAAYKTLSD 82
          DYY LL+V     AS EE+K AYR + + YHPD   DP LK Q+ ++F  ++ AY+ LSD
Sbjct: 14 DYYSLLNVRR--EASSEELKAAYRRLCMLYHPDKHRDPELKSQAERLFNLVHQAYEVLSD 73

Query: 83 PVLRRQYD 91
          P  R  YD
Sbjct: 74 PQTRAIYD 79

[Close]

BLAST of Carg24569 vs. ExPASy TrEMBL
Match: A0A6J1HH09 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111463967 PE=4 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.8e-79
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MVGTPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP 60
           MVGTPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP
Sbjct: 1   MVGTPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP 60

Query: 61  LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILE 120
           LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILE
Sbjct: 61  LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILE 120

Query: 121 LKRRSACQRDRSVSWGGRMRARNYSEC 148
           LKRRSACQRDRSVSWGGRMRARNYSEC
Sbjct: 121 LKRRSACQRDRSVSWGGRMRARNYSEC 147

[Close]

BLAST of Carg24569 vs. ExPASy TrEMBL
Match: A0A6J1HXC8 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111467103 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 4.4e-77
Identity = 144/147 (97.96%), Postives = 144/147 (97.96%), Query Frame = 0


Query: 1   MVGTPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP 60
           MV  PTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP
Sbjct: 1   MVSNPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDP 60

Query: 61  LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILE 120
           LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYER GFEVDRTVWQRQILE
Sbjct: 61  LLKEQSTKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERRGFEVDRTVWQRQILE 120

Query: 121 LKRRSACQRDRSVSWGGRMRARNYSEC 148
           LKRRSACQRDRSVSWGGRMRARNYSEC
Sbjct: 121 LKRRSACQRDRSVSWGGRMRARNYSEC 147

[Close]

BLAST of Carg24569 vs. ExPASy TrEMBL
Match: A0A0A0K7A4 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G061190 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.4e-43
Identity = 98/138 (71.01%), Postives = 113/138 (81.88%), Query Frame = 0


Query: 12  LSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFV 71
           +SCKA+ T+  DYYKLLSVS GCNAS EEIK+AYRAMAL+YHPDLVCDPLLKEQST+MFV
Sbjct: 35  VSCKASATRERDYYKLLSVSGGCNASPEEIKKAYRAMALRYHPDLVCDPLLKEQSTRMFV 94

Query: 72  QLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVDRTVWQRQILELKRRSACQRDR 131
           QLNAAYKTLSDPVLRRQYDDS   LN G++ +  GF  D  VW+RQILELKRRS+ ++DR
Sbjct: 95  QLNAAYKTLSDPVLRRQYDDS---LNMGFNTK--GFRGDSAVWERQILELKRRSSQRKDR 154

Query: 132 S--VSWGGRMRARNYSEC 148
           S   SW  RM+A  +S C
Sbjct: 155 SAPASWAARMQA--HSRC 165

[Close]

BLAST of Carg24569 vs. ExPASy TrEMBL
Match: A0A6J1EIE4 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434524 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 4.6e-42
Identity = 93/140 (66.43%), Postives = 111/140 (79.29%), Query Frame = 0


Query: 6   TRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQ 65
           TRP++ +SC     KG DYY LLSVS GC+AS E+IK+AYRA ALQYHPDLVCDP LK++
Sbjct: 24  TRPVMSVSC-----KGTDYYTLLSVSDGCHASNEDIKKAYRAKALQYHPDLVCDPSLKDK 83

Query: 66  STKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVD-RTVWQRQILELKRR 125
            T+MFVQLNAAYKTLSDPVLRR+YDDS+MGL   +     GF+ D R +WQRQILEL RR
Sbjct: 84  CTRMFVQLNAAYKTLSDPVLRREYDDSLMGLKGNHHGTSTGFKGDYRDLWQRQILELNRR 143

Query: 126 SACQRDRSV-SWGGRMRARN 144
           S  +R+ S+ SWGGRMRA+N
Sbjct: 144 SNLRRNWSLSSWGGRMRAQN 158

[Close]

BLAST of Carg24569 vs. ExPASy TrEMBL
Match: A0A6J1JPJ3 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111486574 PE=4 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 1.0e-41
Identity = 92/144 (63.89%), Postives = 112/144 (77.78%), Query Frame = 0


Query: 6   TRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQ 65
           TRP++ +SC     KG DYY LLSVS GC+AS E+IK+AYR  ALQYHPDLVCDP LK++
Sbjct: 24  TRPVMSVSC-----KGTDYYTLLSVSDGCHASNEDIKKAYRTKALQYHPDLVCDPSLKDK 83

Query: 66  STKMFVQLNAAYKTLSDPVLRRQYDDSMMGLNNGYSYERGGFEVD-RTVWQRQILELKRR 125
            T+MFVQLNAAYKTLSDPVLRR+YDDS+MGL   +     GF+ D R +WQRQILEL +R
Sbjct: 84  CTRMFVQLNAAYKTLSDPVLRREYDDSLMGLKGNHHGTSTGFKGDYRDLWQRQILELNQR 143

Query: 126 SACQRDRSV-SWGGRMRARNYSEC 148
           S  +R+ S+ SWGGRMRA+N  +C
Sbjct: 144 SNLRRNWSLSSWGGRMRAQNRWKC 162

[Close]

BLAST of Carg24569 vs. TAIR 10
Match: AT4G13830.2 (DNAJ-like 20 )

HSP 1 Score: 69.3 bits (168), Expect = 2.9e-12
Identity = 47/127 (37.01%), Postives = 68/127 (53.54%), Query Frame = 0


Query: 24  YYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFVQLNAAYKTLSDP 83
           +Y LL V+   + +  EIK+AY+ +A +YHPD V  P   E+ T  F+++  AY+TLSDP
Sbjct: 67  FYDLLGVTE--SVTLPEIKQAYKQLARKYHPD-VSPPDRVEEYTDRFIRVQEAYETLSDP 126

Query: 84  VLRRQYD-DSMMGLNNGYSYERGGFEVDRTV---------WQRQILELKRRSACQRDRSV 141
             R  YD D  MG +  +S  R        V         WQ Q+  L+RRS  + + ++
Sbjct: 127 RRRVLYDRDLSMGFSFSFSGRRQNRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKDNNTM 186

[Close]

BLAST of Carg24569 vs. TAIR 10
Match: AT2G35720.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 61.6 bits (148), Expect = 6.0e-10
Identity = 39/88 (44.32%), Postives = 53/88 (60.23%), Query Frame = 0


Query: 15  KAATTKGP--DYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLKEQSTKMFVQ 74
           +AA T  P  + Y LL++S    AS EEI++AYR  A  YHPD +  P +KE +T+ F +
Sbjct: 5   EAAPTGPPNRELYALLNLSP--EASDEEIRKAYRQWAQVYHPDKIQSPQMKEVATENFQR 64

Query: 75  LNAAYKTLSDPVLRRQYD-DSMMGLNNG 100
           +  AY+ LSD   R  YD   M GLN+G
Sbjct: 65  ICEAYEILSDETKRLIYDLYGMEGLNSG 90

[Close]

BLAST of Carg24569 vs. TAIR 10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 60.1 bits (144), Expect = 1.8e-09
Identity = 38/87 (43.68%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 4   TPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLK 63
           TP R   G S     T G DYY  L VS   +A+ +EIK AYR +A QYHPD+  +P   
Sbjct: 57  TPYRNRRGRSLVVFATSG-DYYATLGVSK--SANNKEIKAAYRRLARQYHPDVNKEP--- 116

Query: 64  EQSTKMFVQLNAAYKTLSDPVLRRQYD 91
             +T+ F +++AAY+ LSD   R  YD
Sbjct: 117 -GATEKFKEISAAYEVLSDEQKRALYD 136

[Close]

BLAST of Carg24569 vs. TAIR 10
Match: AT1G80030.3 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 60.1 bits (144), Expect = 1.8e-09
Identity = 38/87 (43.68%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 4   TPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLK 63
           TP R   G S     T G DYY  L VS   +A+ +EIK AYR +A QYHPD+  +P   
Sbjct: 57  TPYRNRRGRSLVVFATSG-DYYATLGVSK--SANNKEIKAAYRRLARQYHPDVNKEP--- 116

Query: 64  EQSTKMFVQLNAAYKTLSDPVLRRQYD 91
             +T+ F +++AAY+ LSD   R  YD
Sbjct: 117 -GATEKFKEISAAYEVLSDEQKRALYD 136

[Close]

BLAST of Carg24569 vs. TAIR 10
Match: AT1G80030.2 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 60.1 bits (144), Expect = 1.8e-09
Identity = 38/87 (43.68%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 4   TPTRPMVGLSCKAATTKGPDYYKLLSVSSGCNASGEEIKRAYRAMALQYHPDLVCDPLLK 63
           TP R   G S     T G DYY  L VS   +A+ +EIK AYR +A QYHPD+  +P   
Sbjct: 57  TPYRNRRGRSLVVFATSG-DYYATLGVSK--SANNKEIKAAYRRLARQYHPDVNKEP--- 116

Query: 64  EQSTKMFVQLNAAYKTLSDPVLRRQYD 91
             +T+ F +++AAY+ LSD   R  YD
Sbjct: 117 -GATEKFKEISAAYEVLSDEQKRALYD 136

The following BLAST results are available for this feature:

BLAST of Carg24569 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022963776.1	5.7e-79	100.00	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata] >XP_023511548...	[more]
XP_022967659.1	9.1e-77	97.96	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima]	[more]
XP_038888723.1	6.8e-48	74.82	dnaJ homolog subfamily A member 3, mitochondrial-like [Benincasa hispida]	[more]
KAE8645798.1	3.0e-43	71.01	hypothetical protein Csa_017276 [Cucumis sativus]	[more]
XP_004136866.1	3.0e-43	71.01	chaperone protein dnaJ 20, chloroplastic [Cucumis sativus]	[more]

BLAST of Carg24569 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SDN0	4.1e-11	37.01	Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ2...	[more]
A4XKA5	6.9e-11	47.67	Chaperone protein DnaJ OS=Caldicellulosiruptor saccharolyticus (strain ATCC 4349...	[more]
B5YAR4	6.9e-11	44.83	Chaperone protein DnaJ OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 396...	[more]
Q9NVH1	7.7e-10	48.53	DnaJ homolog subfamily C member 11 OS=Homo sapiens OX=9606 GN=DNAJC11 PE=1 SV=2	[more]
Q5U458	7.7e-10	48.53	DnaJ homolog subfamily C member 11 OS=Mus musculus OX=10090 GN=Dnajc11 PE=1 SV=2	[more]

BLAST of Carg24569 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HH09	2.8e-79	100.00	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1HXC8	4.4e-77	97.96	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A0A0K7A4	1.4e-43	71.01	J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G061190 PE=4 SV=1	[more]
A0A6J1EIE4	4.6e-42	66.43	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1JPJ3	1.0e-41	63.89	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]

BLAST of Carg24569 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13830.2	2.9e-12	37.01	DNAJ-like 20	[more]
AT2G35720.1	6.0e-10	44.32	DNAJ heat shock N-terminal domain-containing protein	[more]
AT1G80030.1	1.8e-09	43.68	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT1G80030.3	1.8e-09	43.68	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT1G80030.2	1.8e-09	43.68	Molecular chaperone Hsp40/DnaJ family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 27..45 score: 32.64 coord: 45..60 score: 44.2 coord: 65..85 score: 31.25
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 22..85 e-value: 1.1E-18 score: 78.1
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 23..90 e-value: 4.2E-19 score: 68.4
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 23..93 score: 18.014336
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 23..82 e-value: 2.92976E-19 score: 74.1189
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 11..109 e-value: 1.3E-22 score: 82.0
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 21..100
None	No IPR available	PANTHER	PTHR45090:SF8	CHAPERONE DNAJ DOMAIN PROTEIN	coord: 11..144
None	No IPR available	PANTHER	PTHR45090	CHAPERONE PROTEIN DNAJ 20 CHLOROPLASTIC	coord: 11..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24569-RA	Carg24569-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061077	chaperone-mediated protein folding
cellular_component	GO:0009507	chloroplast