Carg24552 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24552
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPseudouridine synthase
LocationCarg_Chr11: 68708 .. 72560 (-)
RNA-Seq ExpressionCarg24552
SyntenyCarg24552
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAGCAAAAGGAAAAGGGGAGAAGAGGGCATGGGAGAAGGAGAGATCGTATGGCAAACCCCAGCCAATGCCCCTCAACGATGCGATTACATATTTCACAATGGTACAAACTAAAAGCCCTCTTTTGGGGATTTTCTTTTCGTTTTAGTTTCCAGTTTGGATTAATCAGAGTTCTTTGCTAGGGAGGCGGCACGTTAGACCTTACTACTTCGAGTTCATTGCCCATGTAAGTGCCTTATGGGTTTCCTTTCTTATTTTTCAGTTTAAACTGTCACAAATGTGCGACTATTCAATCTAATTTCAAACTCGTAATTGGAACTTTATATCTACAATTTGCAGGTTAAGAATCGATGGGCCGGCAAATCCATTGTCGACTTGTTTACTGAAGAGTTTAAAGGCCGACCATACGAGTACTATGTATGGTCTTTTTCTTGTTTAATGTGATCATTTGGTCTCAGCTTTTGTCAGTTATTGTATTGTTTGAATCAAAATATAGATTTTTCCTTGTTTTCATGCCTTACTGTGTGGAACTTTGTTTCTCAGATTGATGCAGTCAAATGTGGTCGCATACAAGTCGATGGGGAGATGGTGCCTACATCATATATAGTTAAAACATCTCAAAAGATAAGCCATTTCCTGCACAGGTATATGTTCTCCACGTTGTAGTTTCTGGTAGTATTTTAAAGGCTTATGGTTCACGGTTGGTATAAACTACGTGGCTATGATTCTCTTTAATTTACATTTTTCTGTCTTAATTTTCTTTACTAAAGCTGCATTATAGAGTTTTCAAGCTTGTAATTGATTTCACAAGGGCTGTAGGCATGAACCGCCTGTAATGGCTTTGGAGGTGAAAATTCTCAAAGAAGAAGCAGATGTGCTGACGGTTTACAAGCCTGCGTCTGTTCCAGTGAGTCAGATTGGTAGTATGAATTTCATATGATGTCATTAGTATTGCAAGCTCTGCAATACATGCCCTGTAAACTTTTGAGTTTTAGTGTACACATTCCTCTTGTCTGCCATTGTTTCCCGTCACTTGTGTTTCTATTTAATTAGCAATATCTAATGTAATATAGGTTCATCCATGTGGTCAATATAGGAAGAACACCGTTGTTGGCATACTTCAGGCAGAGCATGGTTTGGCACCTCTATTTCGTATCCAACTTCCATTTCCTTCTTGTAGCTATTATGCTCTTGGCTTAGTTCTGTTAAATTGATAACGAATAAACTCGGTTAGCATACTTTATCCACCGGTTTGAAGGGTAACCCTCTTCTTCCCTTGACATAATCATGTCAGCTATACATCGACTAGACCGTCTTGTCTCAGGACTTCTTATCATTGCTAGAAATGCATCGAAAGCTGATCTTTTCAGACAACAGGTATGCCTTATTCTTAAGACTTTCTTCTTTAGCTATATGTAGTTAATGAAAAGGGAATTCATTTTCATGGAATTTTTATGTCGTGCAGTCTTCTAAAATTTTTTAATCAAACTTGTACAGTGGCTTTTCTTCGGTAGACAACTTCTAACTGTCACATAATGGTTTTCAATGTGTGGACATGTGTCCAGAGCATGTTCGGACTACCATGTCCAACACAAATATTCTGCCTAAAACAAAGTTCTTGAGATTTCTAGATTATGACTTACAAGATATACAAATAATTAATGTTTAGTTTATAAAAAATATTTAAAATATGAATAATTAGGGGATAATTTGGTATTTTAATAAGACAAATGTAAGGAAAACAAAAAAAGTGTAACTCATAAAAATTTGGAAACTGAAGACAATATAAACTGAAGAAGCAATTTTGAAGGAAAAAAAAAAAATCCACTTGCACTGCGAGTTTTTCTCGTTAATAGTCTTGTCCTGTACACTGTAAATAGTTAAATTCAAATTTTCATTGATTTCTCGTTCTTTTCTGCACATGTACAAACTAGGAACTGATGAAACTCACTTAATAGGAATGCTTGCTTTCTGCAGATTGAATCTGGGCTGGTGAAGAAACAGTATATTGCAAGGGTGGTGGGTGTGTTTCCAGAAGAAGAGGTATTTCTTCTTTATTTTAGTCAAGTCATGTTTTTCTCTTCTGTCTTCAATTATTTATCAAACTGTTCCTTCAATTATGACTAGATTAAAGGGTTATTTTTAGATTCTTCTTGTAACAATCTAGAAGAAATCCTTTTGATGAAGGGTAATTCAATATACTCCCTTCGAAATGGAGCTCTGATTGTCTTGTCCTGGTTTGCTGTGGACATGTGCGTGAGTTTTGGCTTGTCGTCTGTGCTCATTATAGGCATGTGGTGTTGTTAGAAGAGTATGAGATCGAACACTCAGTTCTTTTGACACTAAAGGAGTATATATGTTTATCAAATTCTTTGTTCAGTCTCAAGCATAATACCCAGTTATCTGGAGAACTGTGGTGTATTTTTATATTTGGTGAAATGACCTCATAATATTGATATTCTATTTCTATTAGGCATCTATCTGATTATCTGCTTCACTGTATCCTGGATTTGATTTCAGCAAGTGGTCGATGTTCACATAAACTACAATGCTCGAGAGGGAAGGAGCACAGCGGAGGTGAGGCTTCATTTTCTACACTTGTCCATATGGACTACAGTTACTTAAAAAGAGGAAGTGGATAATAAGGGAATGTACATTGTTGACAGTCAAATGTGGTAGGGTCAAACGGTTGTCCTATAAGAACATTCAAAATGCACACAAGTTAACGGTTGTCTTATAGTTGAATTGGTTTGAACTCCCTACGTATAATAAAGAAATTAAAAAGATGGAGTTTAATTCGCAGTGTATTGTTCTGTTTTCCTAAAACAGAACAGTACGGCTATTGTTACAGGTCTAAAACAGGTTTATTCCATAACCATTATATTATCATGGATAAAGTTTGTGAGAGATCTGTTGCTCCTTTGGCTCCTAGTGTCGTCCTCTTGAGTGGAAGCGTATTTGTCTGACTTTAGTATTGACACAACAGATGGTTGATTCTTGCAAGGATACTATGGCAAAGGGGAAGGCCGCTCAAACAAAGTTTACTTGCATCAGTTCCGATGGAGTCCATAGCATTGTCTTATGTGAACCGGTCACAGGACGAACTCATCAAGTAAGTGTAATGTTTGGAATATTGTCCACCCTCTTTTGCATGAGACCTCCCCTTAATGATATTTCGTAAGTTTGAATGATCCATGTATGTTTGCTTCCTCTCCTTTCTTATGTTAAATCGGTGTGTTGTGGTATACCTGCAATGCAGATCCGTGTGCATTTGCAGTACACAGGGTATCCTATTGCTAACGATGCACTGTACCTTACAAAAGTAGCTTCTGGTCGCTCCGTGGAAAAAACAACTGCAGATAGAGCTGCTGCGATATCTGCGTGTTCTACAGCACATGATATTGAAGAAGATTGTGTTAGTGCACGCAAAGAAAACTCAAGTGAAGATTTTAGCATTGACCCCATGTGCACAAACTGTCCAAATTTGGCTCCAAAAGGGTGAGAAACTTTTATTCCACAGGAAGATTTTCTGGTTTGATTCCACTGAAAATGTAACGATGTCCATGAGTGTCGCTTTGAACATGCTTCGTTATGTGCAGATATGACGGAGAAGAAGAGGGATTGTGGCTGCATTGCGTTCGGTACTCCGGACCAGGATGGATCTACGAATGCCCATATCCAGAATGGGCTTCACTTAAATAGACTTCGTATGAAAAGTGTATTTTTTTGGATTCAAGTTTGAGAAGGCTGGATGTGGGTATGAATATGATTCTTCGTAGAGGACCCAGTTTTTGGGATATTTGTGTTTATTATGAAAATGATGAAAATGATGAAAATGATGAAAATGAAG

mRNA sequence

ATGACGAGCAAAAGGAAAAGGGGAGAAGAGGGCATGGGAGAAGGAGAGATCGTATGGCAAACCCCAGCCAATGCCCCTCAACGATGCGATTACATATTTCACAATGTTTGGATTAATCAGAGTTCTTTGCTAGGGAGGCGGCACGTTAGACCTTACTACTTCGAGTTCATTGCCCATGTTAAGAATCGATGGGCCGGCAAATCCATTGTCGACTTGTTTACTGAAGAGTTTAAAGGCCGACCATACGAGTACTATATTGATGCAGTCAAATGTGGTCGCATACAAGTCGATGGGGAGATGGTGCCTACATCATATATAGTTAAAACATCTCAAAAGATAAGCCATTTCCTGCACAGGCATGAACCGCCTGTAATGGCTTTGGAGGTGAAAATTCTCAAAGAAGAAGCAGATGTGCTGACGGTTTACAAGCCTGCGTCTGTTCCAGTTCATCCATGTGGTCAATATAGGAAGAACACCGTTGTTGGCATACTTCAGGCAGAGCATGCTATACATCGACTAGACCGTCTTGTCTCAGGACTTCTTATCATTGCTAGAAATGCATCGAAAGCTGATCTTTTCAGACAACAGATTGAATCTGGGCTGGTGAAGAAACAGTATATTGCAAGGGTGGTGGGTGTGTTTCCAGAAGAAGAGCAAGTGGTCGATGTTCACATAAACTACAATGCTCGAGAGGGAAGGAGCACAGCGGAGAACAGTACGGCTATTGTTACAGGTCTAAAACAGATGGTTGATTCTTGCAAGGATACTATGGCAAAGGGGAAGGCCGCTCAAACAAAGTTTACTTGCATCAGTTCCGATGGAGTCCATAGCATTGTCTTATGTGAACCGGTCACAGGACGAACTCATCAAATCCGTGTGCATTTGCAGTACACAGGGTATCCTATTGCTAACGATGCACTGTACCTTACAAAAGTAGCTTCTGGTCGCTCCGTGGAAAAAACAACTGCAGATAGAGCTGCTGCGATATCTGCGTGTTCTACAGCACATGATATTGAAGAAGATTGTGTTAGTGCACGCAAAGAAAACTCAAGTGAAGATTTTAGCATTGACCCCATGTGCACAAACTGTCCAAATTTGGCTCCAAAAGGATATGACGGAGAAGAAGAGGGATTGTGGCTGCATTGCGTTCGGTACTCCGGACCAGGATGGATCTACGAATGCCCATATCCAGAATGGGCTTCACTTAAATAGACTTCGTATGAAAAGTGTATTTTTTTGGATTCAAGTTTGAGAAGGCTGGATGTGGGTATGAATATGATTCTTCGTAGAGGACCCAGTTTTTGGGATATTTGTGTTTATTATGAAAATGATGAAAATGATGAAAATGATGAAAATGAAG

Coding sequence (CDS)

ATGACGAGCAAAAGGAAAAGGGGAGAAGAGGGCATGGGAGAAGGAGAGATCGTATGGCAAACCCCAGCCAATGCCCCTCAACGATGCGATTACATATTTCACAATGTTTGGATTAATCAGAGTTCTTTGCTAGGGAGGCGGCACGTTAGACCTTACTACTTCGAGTTCATTGCCCATGTTAAGAATCGATGGGCCGGCAAATCCATTGTCGACTTGTTTACTGAAGAGTTTAAAGGCCGACCATACGAGTACTATATTGATGCAGTCAAATGTGGTCGCATACAAGTCGATGGGGAGATGGTGCCTACATCATATATAGTTAAAACATCTCAAAAGATAAGCCATTTCCTGCACAGGCATGAACCGCCTGTAATGGCTTTGGAGGTGAAAATTCTCAAAGAAGAAGCAGATGTGCTGACGGTTTACAAGCCTGCGTCTGTTCCAGTTCATCCATGTGGTCAATATAGGAAGAACACCGTTGTTGGCATACTTCAGGCAGAGCATGCTATACATCGACTAGACCGTCTTGTCTCAGGACTTCTTATCATTGCTAGAAATGCATCGAAAGCTGATCTTTTCAGACAACAGATTGAATCTGGGCTGGTGAAGAAACAGTATATTGCAAGGGTGGTGGGTGTGTTTCCAGAAGAAGAGCAAGTGGTCGATGTTCACATAAACTACAATGCTCGAGAGGGAAGGAGCACAGCGGAGAACAGTACGGCTATTGTTACAGGTCTAAAACAGATGGTTGATTCTTGCAAGGATACTATGGCAAAGGGGAAGGCCGCTCAAACAAAGTTTACTTGCATCAGTTCCGATGGAGTCCATAGCATTGTCTTATGTGAACCGGTCACAGGACGAACTCATCAAATCCGTGTGCATTTGCAGTACACAGGGTATCCTATTGCTAACGATGCACTGTACCTTACAAAAGTAGCTTCTGGTCGCTCCGTGGAAAAAACAACTGCAGATAGAGCTGCTGCGATATCTGCGTGTTCTACAGCACATGATATTGAAGAAGATTGTGTTAGTGCACGCAAAGAAAACTCAAGTGAAGATTTTAGCATTGACCCCATGTGCACAAACTGTCCAAATTTGGCTCCAAAAGGATATGACGGAGAAGAAGAGGGATTGTGGCTGCATTGCGTTCGGTACTCCGGACCAGGATGGATCTACGAATGCCCATATCCAGAATGGGCTTCACTTAAATAG

Protein sequence

MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHVKNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRHEPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHAIHRLDRLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRSTAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVHLQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDFSIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK
Homology
BLAST of Carg24552 vs. NCBI nr
Match: KAG7021380.1 (RNA pseudouridine synthase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 830.9 bits (2145), Expect = 4.8e-237
Identity = 403/403 (100.00%), Postives = 403/403 (100.00%), Query Frame = 0

Query: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHV 60
           MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHV
Sbjct: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHV 60

Query: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120
           KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH
Sbjct: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120

Query: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHAIHRLDRLVSGL 180
           EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHAIHRLDRLVSGL
Sbjct: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHAIHRLDRLVSGL 180

Query: 181 LIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRSTAENST 240
           LIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRSTAENST
Sbjct: 181 LIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRSTAENST 240

Query: 241 AIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVHLQYTGY 300
           AIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVHLQYTGY
Sbjct: 241 AIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVHLQYTGY 300

Query: 301 PIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDFSIDPMC 360
           PIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDFSIDPMC
Sbjct: 301 PIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDFSIDPMC 360

Query: 361 TNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 404
           TNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK
Sbjct: 361 TNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 403

BLAST of Carg24552 vs. NCBI nr
Match: XP_022932600.1 (RNA pseudouridine synthase 7-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 765.4 bits (1975), Expect = 2.5e-217
Identity = 380/409 (92.91%), Postives = 380/409 (92.91%), Query Frame = 0

Query: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHV 60
           MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN         GRRHVRPYYFEFIAHV
Sbjct: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN---------GRRHVRPYYFEFIAHV 60

Query: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120
           KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH
Sbjct: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120

Query: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHA------IHRLD 180
           EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEH       IHRLD
Sbjct: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180

Query: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240
           RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS
Sbjct: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240

Query: 241 TAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300
           T E           MVDSCKDTMAKGKAAQTKFTCI SDGVHSIVLCEPVTGRTHQIRVH
Sbjct: 241 TTE-----------MVDSCKDTMAKGKAAQTKFTCIGSDGVHSIVLCEPVTGRTHQIRVH 300

Query: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360
           LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF
Sbjct: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360

Query: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 404
           SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK
Sbjct: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 389

BLAST of Carg24552 vs. NCBI nr
Match: XP_023531892.1 (RNA pseudouridine synthase 7-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 765.0 bits (1974), Expect = 3.2e-217
Identity = 380/409 (92.91%), Postives = 381/409 (93.15%), Query Frame = 0

Query: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHV 60
           MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN         GRRHVRPYYFEFIAHV
Sbjct: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN---------GRRHVRPYYFEFIAHV 60

Query: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120
           KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH
Sbjct: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120

Query: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHA------IHRLD 180
           EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEH       IHRLD
Sbjct: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180

Query: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240
           RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVG+FPEEEQVVDVHINYNAREGRS
Sbjct: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGMFPEEEQVVDVHINYNAREGRS 240

Query: 241 TAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300
           TAE           MVDSC DTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH
Sbjct: 241 TAE-----------MVDSCMDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300

Query: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360
           LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF
Sbjct: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360

Query: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 404
           SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK
Sbjct: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 389

BLAST of Carg24552 vs. NCBI nr
Match: XP_022974305.1 (RNA pseudouridine synthase 7-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 759.6 bits (1960), Expect = 1.4e-215
Identity = 376/409 (91.93%), Postives = 380/409 (92.91%), Query Frame = 0

Query: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHV 60
           MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN         GRRHVRPYYFEFIAHV
Sbjct: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN---------GRRHVRPYYFEFIAHV 60

Query: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120
           KNRWAGKS+VDLFTEEFKGRPYEYY+DAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH
Sbjct: 61  KNRWAGKSVVDLFTEEFKGRPYEYYVDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120

Query: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHA------IHRLD 180
           EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEH       IHRLD
Sbjct: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180

Query: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240
           RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS
Sbjct: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240

Query: 241 TAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300
           TAE           MVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH
Sbjct: 241 TAE-----------MVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300

Query: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360
           LQYTGYPIANDALYLTKVASGRS+EKTTADRAAAISA STAHDIEEDCV+ARKENSSEDF
Sbjct: 301 LQYTGYPIANDALYLTKVASGRSMEKTTADRAAAISASSTAHDIEEDCVNARKENSSEDF 360

Query: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 404
            IDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK
Sbjct: 361 RIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 389

BLAST of Carg24552 vs. NCBI nr
Match: XP_023531893.1 (RNA pseudouridine synthase 7-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 752.7 bits (1942), Expect = 1.7e-213
Identity = 375/409 (91.69%), Postives = 376/409 (91.93%), Query Frame = 0

Query: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHV 60
           MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN         GRRHVRPYYFEFIAHV
Sbjct: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN---------GRRHVRPYYFEFIAHV 60

Query: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120
           KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH
Sbjct: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120

Query: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHA------IHRLD 180
           EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEH       IHRLD
Sbjct: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180

Query: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240
           RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVG+FPEEEQVVDVHINYNAREGRS
Sbjct: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGMFPEEEQVVDVHINYNAREGRS 240

Query: 241 TAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300
           TAE                 DTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH
Sbjct: 241 TAE-----------------DTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300

Query: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360
           LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF
Sbjct: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360

Query: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 404
           SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK
Sbjct: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 383

BLAST of Carg24552 vs. ExPASy Swiss-Prot
Match: Q0E0Y3 (RNA pseudouridine synthase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0512300 PE=2 SV=2)

HSP 1 Score: 500.4 bits (1287), Expect = 2.0e-140
Identity = 249/402 (61.94%), Postives = 299/402 (74.38%), Query Frame = 0

Query: 13  GEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHVKNRWAGKSIVDL 72
           G   IVWQTPAN P+R DYIF +         GRR+VRPYYFEFI+HVKNRWAGK+IVDL
Sbjct: 4   GPAGIVWQTPANPPERQDYIFRD---------GRRYVRPYYFEFISHVKNRWAGKTIVDL 63

Query: 73  FTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRHEPPVMALEVKIL 132
           FT+EFKGRP EYY+ AVKCGR+QVD +MV   Y+V++SQKISHFLHRHEPPV+  ++ IL
Sbjct: 64  FTDEFKGRPREYYVHAVKCGRLQVDDQMVHADYVVQSSQKISHFLHRHEPPVLGGDITIL 123

Query: 133 KEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHA------IHRLDRLVSGLLIIARN 192
           + EADV+TV KPASVPVHPCGQYRKNTVVGILQAEH       +HRLDRLVSGLLI A+N
Sbjct: 124 QNEADVVTVCKPASVPVHPCGQYRKNTVVGILQAEHGLVPLFPVHRLDRLVSGLLIFAKN 183

Query: 193 ASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRSTAENSTAIVTGL 252
           A KA+ FRQQIE+ L++K+Y+A+VVGVFP+ EQ V+ ++++NAREGRSTAE         
Sbjct: 184 ADKAESFRQQIEASLLQKEYVAKVVGVFPDGEQTVNANVHFNAREGRSTAE--------- 243

Query: 253 KQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVHLQYTGYPIANDA 312
              V         GK A TKF  I +DG+HSIVLC+PVTGRTHQIRVHL++ GYPIAND 
Sbjct: 244 ---VCDGDGKAPIGKQACTKFQRICTDGIHSIVLCKPVTGRTHQIRVHLKHIGYPIANDE 303

Query: 313 LYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSED------FSIDPMC 372
           +YL++  S RS + T  +RA  + ACS      + C      +++ED      FSIDPMC
Sbjct: 304 VYLSENFSPRSSKGTRINRATTL-ACSLPSSDPDSCADLGNNDTNEDTEADEEFSIDPMC 363

Query: 373 TNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASL 403
           TNCPNLAP GYD +EE LWLHCVRY+GP W YECPYP+WA L
Sbjct: 364 TNCPNLAPVGYDADEEALWLHCVRYTGPDWSYECPYPDWAFL 383

BLAST of Carg24552 vs. ExPASy Swiss-Prot
Match: Q9LU60 (RNA pseudouridine synthase 7 OS=Arabidopsis thaliana OX=3702 GN=At5g51140 PE=2 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 6.1e-134
Identity = 243/393 (61.83%), Postives = 285/393 (72.52%), Query Frame = 0

Query: 16  EIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHVKNRWAGKSIVDLFTE 75
           +++ Q+ +   Q+ DYIFH          GRRHVRPYYFEFI+HV  RW GK+IVDLF +
Sbjct: 32  DLLLQSSSKLSQKQDYIFHG---------GRRHVRPYYFEFISHVNKRWTGKTIVDLFAD 91

Query: 76  EFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRHEPPVMALEVKILKEE 135
           EFKGRP +YY+ AVK GRI+VDGE+VP SYIVK+SQKI+HFLHRHEPPVM  +V IL +E
Sbjct: 92  EFKGRPRDYYVGAVKSGRIKVDGEIVPVSYIVKSSQKITHFLHRHEPPVMIDDVVILHQE 151

Query: 136 ADVLTVYKPASVPVHPCGQYRKNTVVGILQAEH------AIHRLDRLVSGLLIIARNASK 195
            DV+TV KPASVPVHPCGQYRKNT+VGIL AEH       IHRLDRLVSGLLIIAR A+K
Sbjct: 152 PDVVTVCKPASVPVHPCGQYRKNTIVGILDAEHDLGTLFPIHRLDRLVSGLLIIARTAAK 211

Query: 196 ADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRSTAENSTAIVTGLKQM 255
           AD FRQQIE G+VKK+YIA+V+GVFPE+E +VD +INYN  EGRSTAE++ +        
Sbjct: 212 ADFFRQQIEGGMVKKRYIAKVIGVFPEDEMIVDANINYNGSEGRSTAEDANS-------- 271

Query: 256 VDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVHLQYTGYPIANDALYL 315
             S  D   KGK A TKFT I ++G HS+V CEPVTGRTHQIRVHLQYTG+PIAND LYL
Sbjct: 272 --SGDDKKVKGKPACTKFTRIDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYL 331

Query: 316 TKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDFSIDPMCTNCPNLAPK 375
                 + ++      A  I A        +D V      SSEDFSIDPMCTNCP L P+
Sbjct: 332 -----NQHIDNLETYIAKRIDAGERKIVSPDDYV-----YSSEDFSIDPMCTNCPKLIPQ 391

Query: 376 GYDGEEEGLWLHCVRYSGPGWIYECPYPEWASL 403
           GY+  +E LWLHCV+Y G GW YECPYP WASL
Sbjct: 392 GYEEHDEALWLHCVQYCGTGWEYECPYPSWASL 395

BLAST of Carg24552 vs. ExPASy Swiss-Prot
Match: Q09709 (Uncharacterized protein C18B11.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC18B11.02c PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.9e-58
Identity = 135/394 (34.26%), Postives = 199/394 (50.51%), Query Frame = 0

Query: 29  CDYIFHNVWINQSSLLGRRHVRPYYFEFIAHVKNRWAGKSIVDLFTEEFKGRPYEYYIDA 88
           C+Y+F           G R V PYY+E+I   K RW GK+++++F  EF+ R   YY  A
Sbjct: 13  CEYVFKGD--------GLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKA 72

Query: 89  VKCGRIQVDGEMVPTSYIVKTSQKISHFLHRHEPPVMALEVKILKEEADVLTVYKPASVP 148
           ++ G+++V+ ++     +++    +SH  HRHEPPV    V I+ E+   + + KPA VP
Sbjct: 73  IRNGQVKVNNQIGNVDTLIENGYIVSHHAHRHEPPVSDQPVGIVHEDESYVVIDKPAGVP 132

Query: 149 VHPCGQYRKNTVVGILQAE------HAIHRLDRLVSGLLIIARNASKADLFRQQIESGLV 208
           VHP G+Y  NTV+ IL  E      +  +RLDRL SGL+  +++   A+  R  + S  +
Sbjct: 133 VHPTGRYNHNTVLHILMKENKCSLLYPCNRLDRLTSGLMFFSKSPKAAEEMRVHMISKSL 192

Query: 209 KKQYIARVVGVFPEEEQVVDVHINYNAREGRSTAENSTAIVTGLKQMVDSCKDTMAKGKA 268
           KK+Y+ARV+G FP   +V+                 + A   GL ++          GK 
Sbjct: 193 KKEYVARVIGEFPNSGEVI-----------CDAPLLTVAPTLGLNRI-------HPDGKE 252

Query: 269 AQTKFTCISSDGVHSIVLCEPVTGRTHQIRVHLQYTGYPIANDALYLTKVASGRSVEK-- 328
           A T F  I+ DG  S+VLC+P+TGRTHQ+RVHLQY GYPIAND +Y  K   G S+ K  
Sbjct: 253 ASTVFKRIAFDGHTSLVLCKPLTGRTHQLRVHLQYLGYPIANDPIYANKRVWGPSLGKNG 312

Query: 329 -----TTADRAAAISACSTAHDI---------EEDCVSARKENSSEDFSIDPMCTNCPNL 388
                +   R + I    TA  +          E    AR++    +      C+ C   
Sbjct: 313 EGSNESVIMRLSEIGKTETASPLLQYEWYTNETEAEAKARRDKRLGELLTGKHCSECGTP 372

Query: 389 APKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWA 401
                  EE G+WLH ++Y    W Y+   P+WA
Sbjct: 373 LYSDPSPEELGIWLHALKYESKDWSYKTQLPKWA 380

BLAST of Carg24552 vs. ExPASy Swiss-Prot
Match: Q8IZ73 (RNA pseudouridylate synthase domain-containing protein 2 OS=Homo sapiens OX=9606 GN=RPUSD2 PE=1 SV=2)

HSP 1 Score: 220.3 bits (560), Expect = 3.9e-56
Identity = 145/422 (34.36%), Postives = 215/422 (50.95%), Query Frame = 0

Query: 45  GRRHVRPYYFEFIAHVKNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMV-PT 104
           G R VRPYYF+F  + K RW G S++ +F+ EF+ +P  YY  AV+ GR+Q++ + V   
Sbjct: 138 GLRKVRPYYFDFRTYCKGRWVGHSLLHVFSTEFRAQPLAYYEAAVRAGRLQLNEKPVQDL 197

Query: 105 SYIVKTSQKISHFLHRHEPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGI 164
           + ++K +  + + +HRHEPPV A  +++L E  DV+ V KP+S+PVHPCG++R NTV+ I
Sbjct: 198 NIVLKDNDFLRNTVHRHEPPVTAEPIRLLAENEDVVVVDKPSSIPVHPCGRFRHNTVIFI 257

Query: 165 LQAE------HAIHRLDRLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEE 224
           L  E      H +HRLDRL SG+L+ A+ A+ ++   +Q+    ++K+Y+ RV G FP E
Sbjct: 258 LGKEHQLKELHPLHRLDRLTSGVLMFAKTAAVSERIHEQVRDRQLEKEYVCRVEGEFPTE 317

Query: 225 EQVVDVHINYNAREGRSTAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHS 284
           E                T +    +V+     V  C+    +GK  +T F  +S +G  S
Sbjct: 318 E---------------VTCKEPILVVS---YKVGVCR-VDPRGKPCETVFQRLSYNGQSS 377

Query: 285 IVLCEPVTGRTHQIRVHLQYTGYPIANDALYLTKVASGRS------VEKT--------TA 344
           +V C P+TGRTHQIRVHLQ+ G+PI ND +Y   VA G S      + KT         A
Sbjct: 378 VVRCRPLTGRTHQIRVHLQFLGHPILNDPIY-NSVAWGPSRGRGGYIPKTNEELLRDLVA 437

Query: 345 DRAA-----AISAC----------STA-------HDIEEDCVSARK-------------- 401
           +  A      +  C          STA        D+EE   +A+K              
Sbjct: 438 EHQAKQSLDVLDLCEGDLSPGLTDSTAPSSELGKDDLEELAAAAQKMEEVAEAAPQELDT 497

BLAST of Carg24552 vs. ExPASy Swiss-Prot
Match: Q149F1 (RNA pseudouridylate synthase domain-containing protein 2 OS=Mus musculus OX=10090 GN=Rpusd2 PE=1 SV=2)

HSP 1 Score: 215.3 bits (547), Expect = 1.3e-54
Identity = 134/416 (32.21%), Postives = 206/416 (49.52%), Query Frame = 0

Query: 45  GRRHVRPYYFEFIAHVKNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMV-PT 104
           G R VRPYYF+F  + K RW G+S++ +F+ EF+ +P  YY  AV+ GR+ ++ E V   
Sbjct: 151 GLRKVRPYYFDFQTYCKGRWVGRSLLHVFSTEFRSQPLSYYEAAVRAGRLHLNEEPVQDL 210

Query: 105 SYIVKTSQKISHFLHRHEPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGI 164
           S ++K +  + + +HRHEPPV A  + +L E  DV+ + KP+S+PVHPCG++R NTV+ I
Sbjct: 211 SIVLKDNDFLRNTVHRHEPPVTAEPIHLLAENNDVVVIDKPSSIPVHPCGRFRHNTVIFI 270

Query: 165 LQAE------HAIHRLDRLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEE 224
           L  E      H +HRLDRL SG+L+ A+ A+ ++   +Q+    ++K+Y+ RV G FP++
Sbjct: 271 LGKEHQLKELHPLHRLDRLTSGVLMFAKTAAVSEKIHEQVRDRQLEKEYVCRVAGEFPDK 330

Query: 225 EQVVDVHINYNAREGRSTAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHS 284
           E +    I                +V   K  V  C+    +GK  +T F  +S +G  S
Sbjct: 331 EVICKEPI----------------LVVSYK--VGVCR-VDPRGKPCETVFQRLSYNGRSS 390

Query: 285 IVLCEPVTGRTHQIRVHLQYTGYPIANDALYLTKV-----ASGRSVEKT----------- 344
           +V C P+TGRTHQIRVHLQ+ G+PI ND +Y +         G  + KT           
Sbjct: 391 VVQCRPLTGRTHQIRVHLQFLGHPILNDPIYNSTAWGPSRGQGGHIPKTDEELLRDLVAE 450

Query: 345 --TADRAAAISACST----------------AHDIEEDCVSARKE--------------- 401
               +  + +  C +                A D  E   +A ++               
Sbjct: 451 HQAKESLSMLDLCESDLTPGLIDSTAPSSELAKDSLEGLATAAQKIDGIAEAAPQHLDTP 510

BLAST of Carg24552 vs. ExPASy TrEMBL
Match: A0A6J1F2L9 (Pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111439108 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.2e-217
Identity = 380/409 (92.91%), Postives = 380/409 (92.91%), Query Frame = 0

Query: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHV 60
           MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN         GRRHVRPYYFEFIAHV
Sbjct: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN---------GRRHVRPYYFEFIAHV 60

Query: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120
           KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH
Sbjct: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120

Query: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHA------IHRLD 180
           EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEH       IHRLD
Sbjct: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180

Query: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240
           RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS
Sbjct: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240

Query: 241 TAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300
           T E           MVDSCKDTMAKGKAAQTKFTCI SDGVHSIVLCEPVTGRTHQIRVH
Sbjct: 241 TTE-----------MVDSCKDTMAKGKAAQTKFTCIGSDGVHSIVLCEPVTGRTHQIRVH 300

Query: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360
           LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF
Sbjct: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360

Query: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 404
           SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK
Sbjct: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 389

BLAST of Carg24552 vs. ExPASy TrEMBL
Match: A0A6J1IDM1 (Pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111472942 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 6.6e-216
Identity = 376/409 (91.93%), Postives = 380/409 (92.91%), Query Frame = 0

Query: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHV 60
           MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN         GRRHVRPYYFEFIAHV
Sbjct: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN---------GRRHVRPYYFEFIAHV 60

Query: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120
           KNRWAGKS+VDLFTEEFKGRPYEYY+DAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH
Sbjct: 61  KNRWAGKSVVDLFTEEFKGRPYEYYVDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120

Query: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHA------IHRLD 180
           EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEH       IHRLD
Sbjct: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180

Query: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240
           RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS
Sbjct: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240

Query: 241 TAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300
           TAE           MVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH
Sbjct: 241 TAE-----------MVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300

Query: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360
           LQYTGYPIANDALYLTKVASGRS+EKTTADRAAAISA STAHDIEEDCV+ARKENSSEDF
Sbjct: 301 LQYTGYPIANDALYLTKVASGRSMEKTTADRAAAISASSTAHDIEEDCVNARKENSSEDF 360

Query: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 404
            IDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK
Sbjct: 361 RIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 389

BLAST of Carg24552 vs. ExPASy TrEMBL
Match: A0A6J1EXG8 (Pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111439108 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 3.1e-213
Identity = 374/409 (91.44%), Postives = 374/409 (91.44%), Query Frame = 0

Query: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHV 60
           MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN         GRRHVRPYYFEFIAHV
Sbjct: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN---------GRRHVRPYYFEFIAHV 60

Query: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120
           KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH
Sbjct: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120

Query: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHA------IHRLD 180
           EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEH       IHRLD
Sbjct: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180

Query: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240
           RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS
Sbjct: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240

Query: 241 TAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300
           T E                 DTMAKGKAAQTKFTCI SDGVHSIVLCEPVTGRTHQIRVH
Sbjct: 241 TTE-----------------DTMAKGKAAQTKFTCIGSDGVHSIVLCEPVTGRTHQIRVH 300

Query: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360
           LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF
Sbjct: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360

Query: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 404
           SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK
Sbjct: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 383

BLAST of Carg24552 vs. ExPASy TrEMBL
Match: A0A6J1I9Y2 (Pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111472942 PE=3 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 1.7e-211
Identity = 370/409 (90.46%), Postives = 374/409 (91.44%), Query Frame = 0

Query: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHV 60
           MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN         GRRHVRPYYFEFIAHV
Sbjct: 1   MTSKRKRGEEGMGEGEIVWQTPANAPQRCDYIFHN---------GRRHVRPYYFEFIAHV 60

Query: 61  KNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120
           KNRWAGKS+VDLFTEEFKGRPYEYY+DAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH
Sbjct: 61  KNRWAGKSVVDLFTEEFKGRPYEYYVDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRH 120

Query: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHA------IHRLD 180
           EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEH       IHRLD
Sbjct: 121 EPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLD 180

Query: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240
           RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS
Sbjct: 181 RLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRS 240

Query: 241 TAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300
           TAE                 DTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH
Sbjct: 241 TAE-----------------DTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVH 300

Query: 301 LQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDF 360
           LQYTGYPIANDALYLTKVASGRS+EKTTADRAAAISA STAHDIEEDCV+ARKENSSEDF
Sbjct: 301 LQYTGYPIANDALYLTKVASGRSMEKTTADRAAAISASSTAHDIEEDCVNARKENSSEDF 360

Query: 361 SIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 404
            IDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK
Sbjct: 361 RIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 383

BLAST of Carg24552 vs. ExPASy TrEMBL
Match: A0A1S3CLH7 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103502297 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.5e-191
Identity = 341/411 (82.97%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 1   MTSKRKRGEE--GMGEGEIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIA 60
           M SKRKR EE   MG G IVWQTPAN PQR DYIF N         GRRHVRPYYFEFIA
Sbjct: 1   MKSKRKREEESMSMGMGGIVWQTPANPPQRSDYIFQN---------GRRHVRPYYFEFIA 60

Query: 61  HVKNRWAGKSIVDLFTEEFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLH 120
           HVKNRWAGK+IVDLF EEFKGRPYEYY+DAVKCGRIQVDGE+VP SYIVK+SQKISHFLH
Sbjct: 61  HVKNRWAGKTIVDLFAEEFKGRPYEYYVDAVKCGRIQVDGEIVPVSYIVKSSQKISHFLH 120

Query: 121 RHEPPVMALEVKILKEEADVLTVYKPASVPVHPCGQYRKNTVVGILQAEHA------IHR 180
           RHEPPVMA  VK+LKEEA+VLTV KPASVPVHPCGQYRKNTVVGIL+AEH       IHR
Sbjct: 121 RHEPPVMAWGVKVLKEEAEVLTVCKPASVPVHPCGQYRKNTVVGILEAEHGLAPLFPIHR 180

Query: 181 LDRLVSGLLIIARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREG 240
           LDRLVSGLLI+ARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVD +INYNAREG
Sbjct: 181 LDRLVSGLLILARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDANINYNAREG 240

Query: 241 RSTAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIR 300
           RSTAE          QMVDS  DT  KGKAAQTKFT ISS+GVHSIVLCEP+TGRTHQIR
Sbjct: 241 RSTAE----------QMVDSSTDTPTKGKAAQTKFTRISSNGVHSIVLCEPITGRTHQIR 300

Query: 301 VHLQYTGYPIANDALYLTKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSE 360
           VHLQYTGYPIANDALYLTK ASGRSV+KTTADRAAAIS+CS A D+EEDC +  KENSSE
Sbjct: 301 VHLQYTGYPIANDALYLTKEASGRSVQKTTADRAAAISSCSPACDVEEDCANEWKENSSE 360

Query: 361 DFSIDPMCTNCPNLAPKGYDGEEEGLWLHCVRYSGPGWIYECPYPEWASLK 404
           DFSIDPMCTNCPNLAPKGY+GEEEGLWLHCVRYSGPGWIYECPYPEWASL+
Sbjct: 361 DFSIDPMCTNCPNLAPKGYEGEEEGLWLHCVRYSGPGWIYECPYPEWASLE 392

BLAST of Carg24552 vs. TAIR 10
Match: AT5G51140.1 (Pseudouridine synthase family protein )

HSP 1 Score: 478.8 bits (1231), Expect = 4.3e-135
Identity = 243/393 (61.83%), Postives = 285/393 (72.52%), Query Frame = 0

Query: 16  EIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHVKNRWAGKSIVDLFTE 75
           +++ Q+ +   Q+ DYIFH          GRRHVRPYYFEFI+HV  RW GK+IVDLF +
Sbjct: 32  DLLLQSSSKLSQKQDYIFHG---------GRRHVRPYYFEFISHVNKRWTGKTIVDLFAD 91

Query: 76  EFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRHEPPVMALEVKILKEE 135
           EFKGRP +YY+ AVK GRI+VDGE+VP SYIVK+SQKI+HFLHRHEPPVM  +V IL +E
Sbjct: 92  EFKGRPRDYYVGAVKSGRIKVDGEIVPVSYIVKSSQKITHFLHRHEPPVMIDDVVILHQE 151

Query: 136 ADVLTVYKPASVPVHPCGQYRKNTVVGILQAEH------AIHRLDRLVSGLLIIARNASK 195
            DV+TV KPASVPVHPCGQYRKNT+VGIL AEH       IHRLDRLVSGLLIIAR A+K
Sbjct: 152 PDVVTVCKPASVPVHPCGQYRKNTIVGILDAEHDLGTLFPIHRLDRLVSGLLIIARTAAK 211

Query: 196 ADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRSTAENSTAIVTGLKQM 255
           AD FRQQIE G+VKK+YIA+V+GVFPE+E +VD +INYN  EGRSTAE++ +        
Sbjct: 212 ADFFRQQIEGGMVKKRYIAKVIGVFPEDEMIVDANINYNGSEGRSTAEDANS-------- 271

Query: 256 VDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVHLQYTGYPIANDALYL 315
             S  D   KGK A TKFT I ++G HS+V CEPVTGRTHQIRVHLQYTG+PIAND LYL
Sbjct: 272 --SGDDKKVKGKPACTKFTRIDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYL 331

Query: 316 TKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDFSIDPMCTNCPNLAPK 375
                 + ++      A  I A        +D V      SSEDFSIDPMCTNCP L P+
Sbjct: 332 -----NQHIDNLETYIAKRIDAGERKIVSPDDYV-----YSSEDFSIDPMCTNCPKLIPQ 391

Query: 376 GYDGEEEGLWLHCVRYSGPGWIYECPYPEWASL 403
           GY+  +E LWLHCV+Y G GW YECPYP WASL
Sbjct: 392 GYEEHDEALWLHCVQYCGTGWEYECPYPSWASL 395

BLAST of Carg24552 vs. TAIR 10
Match: AT5G51140.2 (Pseudouridine synthase family protein )

HSP 1 Score: 457.6 bits (1176), Expect = 1.0e-128
Identity = 235/384 (61.20%), Postives = 277/384 (72.14%), Query Frame = 0

Query: 16  EIVWQTPANAPQRCDYIFHNVWINQSSLLGRRHVRPYYFEFIAHVKNRWAGKSIVDLFTE 75
           +++ Q+ +   Q+ DYIFH          GRRHVRPYYFEFI+HV  RW GK+IVDLF +
Sbjct: 32  DLLLQSSSKLSQKQDYIFHG---------GRRHVRPYYFEFISHVNKRWTGKTIVDLFAD 91

Query: 76  EFKGRPYEYYIDAVKCGRIQVDGEMVPTSYIVKTSQKISHFLHRHEPPVMALEVKILKEE 135
           EFKGRP +YY+ AVK GRI+VDGE+VP SYIVK+SQKI+HFLHRHEPPVM  +V IL +E
Sbjct: 92  EFKGRPRDYYVGAVKSGRIKVDGEIVPVSYIVKSSQKITHFLHRHEPPVMIDDVVILHQE 151

Query: 136 ADVLTVYKPASVPVHPCGQYRKNTVVGILQAEH------AIHRLDRLVSGLLIIARNASK 195
            DV+TV KPASVPVHPCGQYRKNT+VGIL AEH       IHRLDRLVSGLLIIAR A+K
Sbjct: 152 PDVVTVCKPASVPVHPCGQYRKNTIVGILDAEHDLGTLFPIHRLDRLVSGLLIIARTAAK 211

Query: 196 ADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRSTAENSTAIVTGLKQM 255
           AD FRQQIE G+VKK+YIA+V+GVFPE+E +VD +INYN  EGRSTAE++ +        
Sbjct: 212 ADFFRQQIEGGMVKKRYIAKVIGVFPEDEMIVDANINYNGSEGRSTAEDANS-------- 271

Query: 256 VDSCKDTMAKGKAAQTKFTCISSDGVHSIVLCEPVTGRTHQIRVHLQYTGYPIANDALYL 315
             S  D   KGK A TKFT I ++G HS+V CEPVTGRTHQIRVHLQYTG+PIAND LYL
Sbjct: 272 --SGDDKKVKGKPACTKFTRIDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYL 331

Query: 316 TKVASGRSVEKTTADRAAAISACSTAHDIEEDCVSARKENSSEDFSIDPMCTNCPNLAPK 375
                 + ++      A  I A        +D V      SSEDFSIDPMCTNCP L P+
Sbjct: 332 -----NQHIDNLETYIAKRIDAGERKIVSPDDYV-----YSSEDFSIDPMCTNCPKLIPQ 386

Query: 376 GYDGEEEGLWLHCVRYSGPGWIYE 394
           GY+  +E LWLHCV+Y G GW YE
Sbjct: 392 GYEEHDEALWLHCVQYCGTGWEYE 386

BLAST of Carg24552 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 72.0 bits (175), Expect = 1.2e-12
Identity = 74/296 (25.00%), Postives = 127/296 (42.91%), Query Frame = 0

Query: 73  FTEEFKGRPYEYYIDAVKCGRIQVDGEMV-PTSYIVKTSQKISHFLHRHEPPVMALEVKI 132
           FT+     P   +I  ++ G+IQ+DGE+V   + ++++  K+ +     + P     +++
Sbjct: 40  FTKYKSSAPLLGWIQRIQNGQIQIDGEVVKDPNTLLRSGSKLVYSRLPWKEPDTPYSLEV 99

Query: 133 LKEEADVLTVYKPASVPVHPCGQYRKNTV--------------VGILQAEH--AIHRLDR 192
           L E+ D++ + KP+ + V P G +++ TV              +G  ++ H   +HRL R
Sbjct: 100 LYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRLGR 159

Query: 193 LVSGLLIIARN-----------------ASKADLFRQQIESGLVKKQYIARVVGVFPEEE 252
             SG+L+ A+                      +L ++      + K Y A   G+  E+E
Sbjct: 160 GTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADGIVEEDE 219

Query: 253 QVVDVHINYNAREGRSTAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVH-- 312
            V+   I      G         +  GL            +GK A +K   +  D     
Sbjct: 220 VVIKQPIGVVRYPG---------VAQGLYV-------ASPEGKPAFSKVFVLERDREKNC 279

Query: 313 SIVLCEPVTGRTHQIRVHLQYTGYPIANDALYLTKVASG--RSVEKTTADRAAAIS 331
           S+V  E  +GR HQIR+HL Y G+P+  D LY   VA G  +  +    D AAA +
Sbjct: 280 SLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY---VAGGQPKCFDPDLVDDAAAFA 316

BLAST of Carg24552 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 66.2 bits (160), Expect = 6.7e-11
Identity = 64/266 (24.06%), Postives = 114/266 (42.86%), Query Frame = 0

Query: 73  FTEEFKGRPYEYYIDAVKCGRIQVDGEMV-PTSYIVKTSQKISHFLHRHEPPVMALEVKI 132
           FT+     P   +I  ++ G+IQ+DGE+V   + ++++  K+ +     + P     +++
Sbjct: 40  FTKYKSSAPLLGWIQRIQNGQIQIDGEVVKDPNTLLRSGSKLVYSRLPWKEPDTPYSLEV 99

Query: 133 LKEEADVLTVYKPASVPVHPCGQYRKNTV--------------VGILQAEH--AIHRLDR 192
           L E+ D++ + KP+ + V P G +++ TV              +G  ++ H   +HRL R
Sbjct: 100 LYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRLGR 159

Query: 193 LVSGLLIIARN-----------------ASKADLFRQQIESGLVKKQYIARVVGVFPEEE 252
             SG+L+ A+                      +L ++      + K Y A   G+  E+E
Sbjct: 160 GTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADGIVEEDE 219

Query: 253 QVVDVHINYNAREGRSTAENSTAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVH-- 303
            V+   I      G         +  GL            +GK A +K   +  D     
Sbjct: 220 VVIKQPIGVVRYPG---------VAQGLYV-------ASPEGKPAFSKVFVLERDREKNC 279

BLAST of Carg24552 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.1e-08
Identity = 59/251 (23.51%), Postives = 106/251 (42.23%), Query Frame = 0

Query: 94  IQVDGEMV-PTSYIVKTSQKISHFLHRHEPPVMALEVKILKEEADVLTVYKPASVPVHPC 153
           IQ+DGE+V   + ++++  K+ +     + P     +++L E+ D++ + KP+ + V P 
Sbjct: 91  IQIDGEVVKDPNTLLRSGSKLVYSRLPWKEPDTPYSLEVLYEDDDLIALNKPSGLQVLPG 150

Query: 154 GQYRKNTV--------------VGILQAEH--AIHRLDRLVSGLLIIARN---------- 213
           G +++ TV              +G  ++ H   +HRL R  SG+L+ A+           
Sbjct: 151 GLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRLGRGTSGILLCAKTKLAKTKLAAY 210

Query: 214 -------ASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDVHINYNAREGRSTAENS 273
                      +L ++      + K Y A   G+  E+E V+   I      G       
Sbjct: 211 FAEGTSLVGSGNLDQECGTGRKLSKIYRALADGIVEEDEVVIKQPIGVVRYPG------- 270

Query: 274 TAIVTGLKQMVDSCKDTMAKGKAAQTKFTCISSDGVH--SIVLCEPVTGRTHQIRVHLQY 309
             +  GL            +GK A +K   +  D     S+V  E  +GR HQIR+HL Y
Sbjct: 271 --VAQGLYV-------ASPEGKPAFSKVFVLERDREKNCSLVKVEIQSGRPHQIRIHLAY 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021380.14.8e-237100.00RNA pseudouridine synthase 7, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022932600.12.5e-21792.91RNA pseudouridine synthase 7-like isoform X1 [Cucurbita moschata][more]
XP_023531892.13.2e-21792.91RNA pseudouridine synthase 7-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022974305.11.4e-21591.93RNA pseudouridine synthase 7-like isoform X1 [Cucurbita maxima][more]
XP_023531893.11.7e-21391.69RNA pseudouridine synthase 7-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q0E0Y32.0e-14061.94RNA pseudouridine synthase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g05... [more]
Q9LU606.1e-13461.83RNA pseudouridine synthase 7 OS=Arabidopsis thaliana OX=3702 GN=At5g51140 PE=2 S... [more]
Q097091.9e-5834.26Uncharacterized protein C18B11.02c OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
Q8IZ733.9e-5634.36RNA pseudouridylate synthase domain-containing protein 2 OS=Homo sapiens OX=9606... [more]
Q149F11.3e-5432.21RNA pseudouridylate synthase domain-containing protein 2 OS=Mus musculus OX=1009... [more]
Match NameE-valueIdentityDescription
A0A6J1F2L91.2e-21792.91Pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111439108 PE=3 SV=1[more]
A0A6J1IDM16.6e-21691.93Pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111472942 PE=3 SV=1[more]
A0A6J1EXG83.1e-21391.44Pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111439108 PE=3 SV=1[more]
A0A6J1I9Y21.7e-21190.46Pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111472942 PE=3 SV=1[more]
A0A1S3CLH71.5e-19182.97Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103502297 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G51140.14.3e-13561.83Pseudouridine synthase family protein [more]
AT5G51140.21.0e-12861.20Pseudouridine synthase family protein [more]
AT3G52260.21.2e-1225.00Pseudouridine synthase family protein [more]
AT3G52260.16.7e-1124.06Pseudouridine synthase family protein [more]
AT3G52260.31.1e-0823.51Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 122..385
e-value: 4.9E-46
score: 159.0
NoneNo IPR availablePANTHERPTHR21600:SF62PSEUDOURIDINE SYNTHASEcoord: 14..402
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 14..402
NoneNo IPR availableCDDcd02557PseudoU_synth_ScRIB2coord: 114..401
e-value: 3.01378E-90
score: 269.5
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 137..296
e-value: 3.1E-26
score: 92.4
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 170..184
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 121..399

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24552-RACarg24552-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding