Carg24507 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24507
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionserine/arginine-rich splicing factor SC35
LocationCarg_Chr19: 2009616 .. 2014104 (+)
RNA-Seq ExpressionCarg24507
SyntenyCarg24507
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTTCTTCTCCGTCGTCTCTAACCCAAACCCTAATCTTCATCTTCCAACCAACATGTCGCACTTCGGAAGGTCCGGTCCTCCAGATATTGCAGACACCTATTCGCTTCTCGTGCTCAACATTACCTTCCGTATGATTCCATATATCTCTCTTTTTCTTTCTCAAATTTCCTTGTTTCTTTTGTGTTTTCCTGTTTTGTATCGTATTCAGTCCCCACTGAGATCTTTTTTGTTTTTTTTTTGTGTTTTTAGGAACCACCGCTGATGATCTCTACCCGCTGTTCCACAAGTATGGTAAGGTTGTCGATATCTTCATCCCCAGAGACCGAAGGTATGATTCCCTGCTTTGTTGCGTTTGTTTTCTTATGAAATTTGTTCGATTTCTGTGAGAGATGACGATGAATGTTTTTTTTAATAGCTGGTAAGAGTTTTGTGTTTGTTAGATTAAACATTTTGAGGTTAGGATTGAATTGATTTCCTTCCATTGATATTCATCTGGTTCCTATGAAATTAGTTTGCACGTGAGGGTGAAATGATATTCTTCATTCAGCTTAAATTATGCTTGGGCTCAGTTGGGGGCTTTATTTATTTTTCTGTTTGTTCATCTTTTGTTAGCCATCCATCAATTATTCTCGTTTTGATTATTTCGTAATTGTCTCTTTTATTGTATCGTGGTTCAGAACTGGTGATTCTAGAGGTTTTGCATTTGTGCGTTACAAGTATGCAGATGAGGCTCAGAAAGCAGTGGAAAGGCTAGATGGTACCCCTGCCTCTTTGTTTGTTTCCTGTTGTTTGATTTTGTCCTTTTCCTCTTCAAGTGGAAGTAATTTGTAGATACAAGTTACAAGGAAGATGTTTTATATTTTTGTTGGACTGAAGGCTGATAATCTTATGGTCGTTATTCAAATCAGTTTGGGCGGGCCCTATAATATCAATATTCTCTGAATTAACTCCCTCACTTCTTTGTTAGTTCTGGTTTTGAATTACAATCATGCCTCTACTTACTTCCATTTGATCATTTCTATGCAGTTGGTTTGGGGAGCTAATCTGCTGCATATAAATTTGAAGCTGTATATATAATTAATTAATTATTTTTTTAAATTTTGTCATGTTCCAGGTATTGTGGTTGATGGACGAGAGATCACCGTTCAGTTTGCAAAATATGGTCCAAATGCAGAAAGGATGTGAGTAATGAAGTAGTGGTTTTCCTCGATGAATGCTCACGTTTGGGCCTAACTTCTTAACTCTTACATATTTGTTTTGCTAATTGGTGTTGTATTTATGTCAAAATATGCTTTTAGTCACAAAGGAAAGGTCAGTGAATCATTTCCAAAGTCGAGATACAGGTCTAGAAGTCGCAGTCCACGGAGACGGTAATTTTCTCTTCCACATCCCTATGAATTTTTTATATTGGGATTAAATATTTGTTGTCCTTATCCTTGAAGAATATTGTCTGGTCATTAGAGTTCCTTGGTGGAATTATTATTTCTTCAGCTTTAAGATGTTCAAGGGCGAAAGCTCCGGTTGAAATAGGATATTCGAGGAATATGATGCAGCCACATTTTTGAACCCTAAGATATTGTGTTAACTGTTTGTGCCTAGGATTACTTTAGCAAGCTCTTGTTTTCTGCTATCTCAGATATGAGGTTTGAAGATGCTGTTTTGTCCAAGTTTTTCATTCTTTTTTAGGTTAGATATGGTATGCTGTTTTAACATGAGATACTAGAATTATGGAAATGTAAAATGTTGGAAGATCTAAAAGGAGACTGTAGCAGTGAATTCTATATATGGCTATGAGTAGATAGTGGTGCTCGAACTTTTTCTTTCATGGAGTTCAAATTTTGACCATTTGCATGTAAAGTAACTACTTGGAATTTCTTACTTTCTTTCCATTAAGGTCCATTTCTTTATGAGCTTTTGTAGTTTTATCTTTATTTTTTAAAATTCAAGTTCCTAATTGAAATTTATTACTTTCAGACATCGGGATGATCACAGAGATAGGGATTATAGGAGAAGAAGTCGAAGCAGAAGTAGAAGCAGAAGCTTGGACAGGATTGATCATGATAGGCGTGGAAGAGAAAGGGAGAGGGAAAGAGACTATCGTAGGCGAAGCCGGAGCATCAGTCCTAGGCGGCCTAGTCCTGATTATTCCAAGGGGAGGGGAAGAGGACGCTACATTGACGAGCGTAGAAGCCGCAGCCGCAGCCGTAGCCGTAGCCATAGCCATAGCCATAGCCAAAGTCGTGGCCGTGGTCGTGGCCGTTCTGTGGATAGGTTGTTAATTTCTTACAATTTTTATCCAGTTGGAAGAAAGTTGGGAATTGTATTTCTAATCATCTTTATCCATTTGGGAAAAAGTGGGGAAAATCTTATTTCTAATCAGTCATTGTTCTACATATTACAGTGCTTCTCCTGGTCGACAAAGCCCTAGTCCAAGGAGTGCATCTCCTCAAAGGAGTCCATCTCCCAGGGGTGAAAGTCCTGAAAGAAATGGTGATGGTGGAAGGTCTCCAACTTCCAGGAGTGTTTCACCCCGAGGTCAACCTGCTGATTCAAGAAGCCCATCTCAACCAAATTCAGATGTTGATGTGAGTTGCATTATGAACTATTTTGAATGACTAGTCTAGACTTGGTGTTAGTAGTTAAATGTTTCACTTGTCTTAATGTTGACAGAAGTGAATACACTGATGGATGCAAGGAGAACTGTTGACATTTCCGAGCAGTGCAGGAATGGATGCTTTGAGGACTCGTTGATTAAAAATGTTATCAGACTATAAGTTCTAGTTTTGGTAGGACTTTCCTATGGTGTTCAATTCTCATATATTGAGCTTAATGTGCCTATCTACTATCTTGTATCAGTTTGATACTTTTTGGCTCTGCTGTCTGTATCCTTATATCCAGTCATAGGATATAGCTTGGGACATGTTCTTCCTCCTTTATATTTCTCCTCTCCACCCTGTAGTACTAGTAATATTTTATCTTGAGCAATTGTGGTGTCTTTTTCTTTTTCCCATTTTCCTTTTTTTTTAATAAATGTTTTAGCTATCCTGATTCATCATTGAATCCTCTATTGGATTTTATAGTTATGTTCGGGGTAAGAGTGACCGGCGCCTGATTTGGCCGATGTTGATTTTGTGGCTCTTGATGCTGTTTCTGTGTGATAGATGGGTGATCGTGACTTTGATGCTGGTTGCAGCAGTTTGCTGATTTATCAGTATGATTGTCTATGCAGTATGATGTTGCTGTGGACAGTGTCTTAACTAATATTCACTGGATGAAAAAATTATTGGTAAGGTTTTGGCCACATTTTAAATGTTTTTAAAAACTTGATAATGCTTTTAGGATTTGATGTTTGGCCTGATTATTCATTCTGGTCTATCCAAGTTTTGAAAGTTGCACCTCACCTGCCAAGGAGATCTAGAGGCTTTGATATATAGGGTTGACCCTCCTCTGTTGATCGAATTTTACTCATAGCTGATGATGCATTTGAGATCGGTATTGATTTTAATGGCGAAGGCTGGATTAAGAAAACTTAGCTGCAGGTTTGATCTTTGCGTCCGAACATGATTGGCCACGGTAACATAAGTTTATAGTTTTGTGTATTAGTTTATAGTTGTGTATTATCCGAACATGACAGGTTAGTATCCCAAACATTTAAATCAATCTCTTGCTTGTTGCTTGATGTGGTGACTGACTTATTGTAGCTTTTGGATACACAATTGTTGCGGTGGTGTTTGATAGCAGATATCTTTATTGATGTACGATAGTCATCGGTTCGATTGATTGATTGATTCTGAAGTGCTAACGCTGTAGCTGGCTGTTGCTGCCCTTGTGGGGGTGAGGCTGGCAATGATGTTTGCAGTTTGCTTTGACAATGGATGCATTGATCTTTGCTTGCCTCAATATAAAGTTCTGCACGTATCGCTTGCTTCACGTCTGTAAGAAAATTTGCTTGATAAAAAATTCTGACATTGATTTGCAACATTTGGTCCGATTGCTAAATAAATTTAGTATGGTGAACACGTATGACTACTTGCTTGGTGAACACGATTCCTATATTGCTTTTAGCAGCAACTAGGTTTTGGATTTGGAAATACTGCCTATCTGATATTTTATCTATTGCTCGAAAGAGAGATGCTTTAACTGTGGCAGGAAGCAAGCTTGTTAGATTGCTTGCATCCTTTTTATGGTTTATTGGATTCCAGAGTCTGCTTTTAGATGCTTGCAGGTTTTTTACGTCAACAATTTTGTTTGCTCTAATTTTTGTTGAGTTTTGGTCTTTGCTTCTACCTTTTATCCAAACAAAATATGAGGAACATAGACAAGCATTTCCAATGTTATCAGGTATGTTGTGTTGTGTTTGATCATTGATGGTCCAATTTGTTAAAGGTCTACATAGTTGAGGAATTTTGATATGTTGTCTGCATTGTGTGGCTGTCTCTTTATTTATTTTTTTTTATCAAGTAATAATAATTGGAGTGGAAGATTAA

mRNA sequence

CCTTTCTTCTCCGTCGTCTCTAACCCAAACCCTAATCTTCATCTTCCAACCAACATGTCGCACTTCGGAAGGTCCGGTCCTCCAGATATTGCAGACACCTATTCGCTTCTCGTGCTCAACATTACCTTCCGAACCACCGCTGATGATCTCTACCCGCTGTTCCACAAGTATGGTAAGGTTGTCGATATCTTCATCCCCAGAGACCGAAGAACTGGTGATTCTAGAGGTTTTGCATTTGTGCGTTACAAGTATGCAGATGAGGCTCAGAAAGCAGTGGAAAGGCTAGATGGTATTGTGGTTGATGGACGAGAGATCACCGTTCAGTTTGCAAAATATGGTCCAAATGCAGAAAGGATTCACAAAGGAAAGGTCAGTGAATCATTTCCAAAGTCGAGATACAGGTCTAGAAGTCGCAGTCCACGGAGACGACATCGGGATGATCACAGAGATAGGGATTATAGGAGAAGAAGTCGAAGCAGAAGTAGAAGCAGAAGCTTGGACAGGATTGATCATGATAGGCGTGGAAGAGAAAGGGAGAGGGAAAGAGACTATCGTAGGCGAAGCCGGAGCATCAGTCCTAGGCGGCCTAGTCCTGATTATTCCAAGGGGAGGGGAAGAGGACGCTACATTGACGAGCGTAGAAGCCGCAGCCGCAGCCGTAGCCGTAGCCATAGCCATAGCCATAGCCAAAGTCGTGGCCGTGGTCGTGGCCGTTCTGTGGATAGTGCTTCTCCTGGTCGACAAAGCCCTAGTCCAAGGAGTGCATCTCCTCAAAGGAGTCCATCTCCCAGGGGTGAAAGTCCTGAAAGAAATGGTGATGGTGGAAGGTCTCCAACTTCCAGGAGTGTTTCACCCCGAGGTCAACCTGCTGATTCAAGAAGCCCATCTCAACCAAATTCAGATGTTGATTTTATAGTTGTGTATTATCCGAACATGACAGTAATAATAATTGGAGTGGAAGATTAA

Coding sequence (CDS)

ATGTCGCACTTCGGAAGGTCCGGTCCTCCAGATATTGCAGACACCTATTCGCTTCTCGTGCTCAACATTACCTTCCGAACCACCGCTGATGATCTCTACCCGCTGTTCCACAAGTATGGTAAGGTTGTCGATATCTTCATCCCCAGAGACCGAAGAACTGGTGATTCTAGAGGTTTTGCATTTGTGCGTTACAAGTATGCAGATGAGGCTCAGAAAGCAGTGGAAAGGCTAGATGGTATTGTGGTTGATGGACGAGAGATCACCGTTCAGTTTGCAAAATATGGTCCAAATGCAGAAAGGATTCACAAAGGAAAGGTCAGTGAATCATTTCCAAAGTCGAGATACAGGTCTAGAAGTCGCAGTCCACGGAGACGACATCGGGATGATCACAGAGATAGGGATTATAGGAGAAGAAGTCGAAGCAGAAGTAGAAGCAGAAGCTTGGACAGGATTGATCATGATAGGCGTGGAAGAGAAAGGGAGAGGGAAAGAGACTATCGTAGGCGAAGCCGGAGCATCAGTCCTAGGCGGCCTAGTCCTGATTATTCCAAGGGGAGGGGAAGAGGACGCTACATTGACGAGCGTAGAAGCCGCAGCCGCAGCCGTAGCCGTAGCCATAGCCATAGCCATAGCCAAAGTCGTGGCCGTGGTCGTGGCCGTTCTGTGGATAGTGCTTCTCCTGGTCGACAAAGCCCTAGTCCAAGGAGTGCATCTCCTCAAAGGAGTCCATCTCCCAGGGGTGAAAGTCCTGAAAGAAATGGTGATGGTGGAAGGTCTCCAACTTCCAGGAGTGTTTCACCCCGAGGTCAACCTGCTGATTCAAGAAGCCCATCTCAACCAAATTCAGATGTTGATTTTATAGTTGTGTATTATCCGAACATGACAGTAATAATAATTGGAGTGGAAGATTAA

Protein sequence

MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSRSPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSPDYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQRSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVDFIVVYYPNMTVIIIGVED
Homology
BLAST of Carg24507 vs. NCBI nr
Match: KAG7011405.1 (Serine/arginine-rich splicing factor SC35 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 561.6 bits (1446), Expect = 4.1e-156
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180
           SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQ 240
           DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQ
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQ 240

Query: 241 RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVDFIVVYYPNMTVIIIG 300
           RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVDFIVVYYPNMTVIIIG
Sbjct: 241 RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVDFIVVYYPNMTVIIIG 300

Query: 301 VED 304
           VED
Sbjct: 301 VED 303

BLAST of Carg24507 vs. NCBI nr
Match: KAG6571679.1 (Serine/arginine-rich splicing factor SC35, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 526.6 bits (1355), Expect = 1.5e-145
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 218 MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 277

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 278 FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 337

Query: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180
           SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP
Sbjct: 338 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 397

Query: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQ 240
           DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQ
Sbjct: 398 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQ 457

Query: 241 RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286
           RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD
Sbjct: 458 RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 502

BLAST of Carg24507 vs. NCBI nr
Match: XP_022963472.1 (serine/arginine-rich splicing factor SC35-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 521.5 bits (1342), Expect = 4.7e-144
Identity = 283/285 (99.30%), Postives = 283/285 (99.30%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180
           SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQ 240
           DYSKGRGRGRYIDERRSRSRSRSRS SHSHSQSRGRGRGRSVDSASPGRQSPSPRSASP 
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSRSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPH 240

Query: 241 RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286
           RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD
Sbjct: 241 RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 285

BLAST of Carg24507 vs. NCBI nr
Match: XP_022963473.1 (serine/arginine-rich splicing factor SC35-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 515.0 bits (1325), Expect = 4.4e-142
Identity = 282/286 (98.60%), Postives = 282/286 (98.60%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180
           SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDS-ASPGRQSPSPRSASP 240
           DYSKGRGRGRYIDERRSRSRSRSRS SHSHSQSRGRGRGRSVD  ASPGRQSPSPRSASP
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSRSHSHSQSRGRGRGRSVDRFASPGRQSPSPRSASP 240

Query: 241 QRSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286
            RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD
Sbjct: 241 HRSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286

BLAST of Carg24507 vs. NCBI nr
Match: XP_022963471.1 (serine/arginine-rich splicing factor SC35-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 515.0 bits (1325), Expect = 4.4e-142
Identity = 282/286 (98.60%), Postives = 282/286 (98.60%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180
           SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDS-ASPGRQSPSPRSASP 240
           DYSKGRGRGRYIDERRSRSRSRSRS SHSHSQSRGRGRGRSVD  ASPGRQSPSPRSASP
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSRSHSHSQSRGRGRGRSVDRFASPGRQSPSPRSASP 240

Query: 241 QRSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286
            RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD
Sbjct: 241 HRSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286

BLAST of Carg24507 vs. ExPASy Swiss-Prot
Match: Q9FMG4 (Serine/arginine-rich splicing factor SC35 OS=Arabidopsis thaliana OX=3702 GN=SC35 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 5.9e-65
Identity = 179/303 (59.08%), Postives = 198/303 (65.35%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDI+DTYSLLVLNITFRTTADDLYPLF KYGKVVD+FIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDISDTYSLLVLNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYR---- 120
           FVRYKY DEA KAVERLDG VVDGREITVQFAKYGPNAE+I KG+V E  PKSR      
Sbjct: 61  FVRYKYKDEAHKAVERLDGRVVDGREITVQFAKYGPNAEKISKGRVVEPPPKSRRSRSRS 120

Query: 121 -------------------SRSRSPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRG 180
                               RSRSPRRR RDD+R++DYR+RSRSRS         +DRR 
Sbjct: 121 PRRSRSPRRSRSPPRRRSPRRSRSPRRRSRDDYREKDYRKRSRSRS---------YDRRE 180

Query: 181 RERERERDYRRRSRSISPRRPSPDYSKGRGRGRYIDERRSRSRSRS-----RSHSHSHSQ 240
           R  E++RD+RRR+RS   R  SPD  K R RGRY +E RS SRS S     RS   S  Q
Sbjct: 181 RHEEKDRDHRRRTRS---RSASPD-EKRRVRGRYDNESRSHSRSLSASPARRSPRSSSPQ 240

Query: 241 SRGRGRGRSVDSASPGRQSPSPRSASPQRSPSPRGESPERNGDGGRSPTSRSVSPRGQPA 276
                R  S D  S   +SPSPR +   RSP+ +  SP +     R    RS SP G PA
Sbjct: 241 KTSPAREVSPDKRS-NERSPSPRRSLSPRSPALQKASPSKEMSPERRSNERSPSP-GSPA 288

BLAST of Carg24507 vs. ExPASy Swiss-Prot
Match: P30352 (Serine/arginine-rich splicing factor 2 OS=Gallus gallus OX=9031 GN=SRSF2 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-27
Identity = 106/248 (42.74%), Postives = 137/248 (55.24%), Query Frame = 0

Query: 4   FGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVR 63
           +GR  PPD+    SL V N+T+RT+ D L  +F KYG+V D++IPRDR T +SRGFAFVR
Sbjct: 3   YGRP-PPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVR 62

Query: 64  YKYADEAQKAVERLDGIVVDGREITVQFAKYG--PNAERIHKGKVSESFPKSRYRSRSRS 123
           +    +A+ A++ +DG V+DGRE+ VQ A+YG  P++    +G     +  S Y  RSRS
Sbjct: 63  FHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGSSGYGRRSRS 122

Query: 124 PRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSPD 183
           PRR           RRRSRSRSRSRS          R R R R  R +SR          
Sbjct: 123 PRR-----------RRRSRSRSRSRS----------RSRSRSRYSRSKSR---------- 182

Query: 184 YSKGRGRGRYIDERRS--RSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASP 243
            S+ R R R   + RS  RS+S+S S S S S+SR R R RS    S    +   RS SP
Sbjct: 183 -SRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPTSKRESNSRSRSKSP 217

Query: 244 QRSPSPRG 248
            +SP   G
Sbjct: 243 PKSPEEEG 217

BLAST of Carg24507 vs. ExPASy Swiss-Prot
Match: Q06A98 (Serine/arginine-rich splicing factor 2 OS=Sus scrofa OX=9823 GN=SRSF2 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 3.7e-27
Identity = 106/248 (42.74%), Postives = 136/248 (54.84%), Query Frame = 0

Query: 4   FGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVR 63
           +GR  PPD+    SL V N+T+RT+ D L  +F KYG+V D++IPRDR T +SRGFAFVR
Sbjct: 3   YGRP-PPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVR 62

Query: 64  YKYADEAQKAVERLDGIVVDGREITVQFAKYG--PNAERIHKGKVSESFPKSRYRSRSRS 123
           +    +A+ A++ +DG V+DGRE+ VQ A+YG  P++    +G     +    Y  RSRS
Sbjct: 63  FHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRS 122

Query: 124 PRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSPD 183
           PRR           RRRSRSRSRSRS          R R R R  R +SR          
Sbjct: 123 PRR-----------RRRSRSRSRSRS----------RSRSRSRYSRSKSR---------- 182

Query: 184 YSKGRGRGRYIDERRS--RSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASP 243
            S+ R R R   + RS  RS+S+S S S S S+SR R R RS   AS        RS SP
Sbjct: 183 -SRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPASKRESKSRSRSKSP 217

Query: 244 QRSPSPRG 248
            +SP   G
Sbjct: 243 PKSPEEEG 217

BLAST of Carg24507 vs. ExPASy Swiss-Prot
Match: Q5R1W5 (Serine/arginine-rich splicing factor 2 OS=Pan troglodytes OX=9598 GN=SRSF2 PE=2 SV=3)

HSP 1 Score: 122.9 bits (307), Expect = 6.4e-27
Identity = 108/253 (42.69%), Postives = 139/253 (54.94%), Query Frame = 0

Query: 4   FGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVR 63
           +GR  PPD+    SL V N+T+RT+ D L  +F KYG+V D++IPRDR T +SRGFAFVR
Sbjct: 3   YGRP-PPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVR 62

Query: 64  YKYADEAQKAVERLDGIVVDGREITVQFAKYG--PNAERIHKGKVSESFPKSRYRSRSRS 123
           +    +A+ A++ +DG V+DGRE+ VQ A+YG  P++    +G     +    Y  RSRS
Sbjct: 63  FHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRS 122

Query: 124 PRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSPD 183
           PRR           RRRSRSRSRSRS          R R R R  R +SR          
Sbjct: 123 PRR-----------RRRSRSRSRSRS----------RSRSRSRYSRSKSR---------- 182

Query: 184 YSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQR 243
            S+ R R R   + RS  RS+S+S S S S+SR R R RS       ++ P  RS    R
Sbjct: 183 -SRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPVSKREPKSRS----R 217

Query: 244 SPSPRGESPERNG 255
           S SP  ESPE  G
Sbjct: 243 SKSP-PESPEEEG 217

BLAST of Carg24507 vs. ExPASy Swiss-Prot
Match: Q3MHR5 (Serine/arginine-rich splicing factor 2 OS=Bos taurus OX=9913 GN=SRSF2 PE=2 SV=3)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-26
Identity = 105/248 (42.34%), Postives = 135/248 (54.44%), Query Frame = 0

Query: 4   FGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVR 63
           +GR  PPD+    SL V N+T+RT+ D L  +F KYG+V D++IPRDR T +SRGFAFVR
Sbjct: 3   YGRP-PPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVR 62

Query: 64  YKYADEAQKAVERLDGIVVDGREITVQFAKYG--PNAERIHKGKVSESFPKSRYRSRSRS 123
           +    +A+ A++ +DG V+DGRE+ VQ A+YG  P++    +G     +    Y  RSRS
Sbjct: 63  FHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRS 122

Query: 124 PRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSPD 183
           PRR           RRRSRSRSRSRS          R R R R  R +SR          
Sbjct: 123 PRR-----------RRRSRSRSRSRS----------RSRSRSRYSRSKSR---------- 182

Query: 184 YSKGRGRGRYIDERRS--RSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASP 243
            S+ R R R   + RS  RS+S+S S S S S+SR R R RS    S        RS SP
Sbjct: 183 -SRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPVSKRESKSRSRSKSP 217

Query: 244 QRSPSPRG 248
            +SP   G
Sbjct: 243 PKSPEEEG 217

BLAST of Carg24507 vs. ExPASy TrEMBL
Match: A0A6J1HFC7 (serine/arginine-rich splicing factor SC35-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111463797 PE=4 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.3e-144
Identity = 283/285 (99.30%), Postives = 283/285 (99.30%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180
           SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQ 240
           DYSKGRGRGRYIDERRSRSRSRSRS SHSHSQSRGRGRGRSVDSASPGRQSPSPRSASP 
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSRSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPH 240

Query: 241 RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286
           RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD
Sbjct: 241 RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 285

BLAST of Carg24507 vs. ExPASy TrEMBL
Match: A0A6J1HG77 (serine/arginine-rich splicing factor SC35-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463797 PE=4 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.1e-142
Identity = 282/286 (98.60%), Postives = 282/286 (98.60%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180
           SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDS-ASPGRQSPSPRSASP 240
           DYSKGRGRGRYIDERRSRSRSRSRS SHSHSQSRGRGRGRSVD  ASPGRQSPSPRSASP
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSRSHSHSQSRGRGRGRSVDRFASPGRQSPSPRSASP 240

Query: 241 QRSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286
            RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD
Sbjct: 241 HRSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286

BLAST of Carg24507 vs. ExPASy TrEMBL
Match: A0A6J1HHW9 (serine/arginine-rich splicing factor SC35-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111463797 PE=4 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.1e-142
Identity = 282/286 (98.60%), Postives = 282/286 (98.60%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180
           SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDS-ASPGRQSPSPRSASP 240
           DYSKGRGRGRYIDERRSRSRSRSRS SHSHSQSRGRGRGRSVD  ASPGRQSPSPRSASP
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSRSHSHSQSRGRGRGRSVDRFASPGRQSPSPRSASP 240

Query: 241 QRSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286
            RSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD
Sbjct: 241 HRSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286

BLAST of Carg24507 vs. ExPASy TrEMBL
Match: A0A6J1HVK2 (serine/arginine-rich splicing factor SC35-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467060 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.2e-137
Identity = 276/286 (96.50%), Postives = 279/286 (97.55%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSR RSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRNRSRSR 120

Query: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180
           SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSP-RSASP 240
           DYSKGRGRGRY+DERRSRSRSR    SHS+SQ+RGRGRGRSVDSASPGRQSPSP RSASP
Sbjct: 181 DYSKGRGRGRYVDERRSRSRSR----SHSYSQTRGRGRGRSVDSASPGRQSPSPRRSASP 240

Query: 241 QRSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286
           QRSPSPRGES ERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD
Sbjct: 241 QRSPSPRGESAERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 282

BLAST of Carg24507 vs. ExPASy TrEMBL
Match: A0A6J1HX79 (serine/arginine-rich splicing factor SC35-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111467060 PE=4 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.1e-135
Identity = 275/287 (95.82%), Postives = 278/287 (96.86%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSR RSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRNRSRSR 120

Query: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180
           SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDS-ASPGRQSPSP-RSAS 240
           DYSKGRGRGRY+DERRSRSRSR    SHS+SQ+RGRGRGRSVD  ASPGRQSPSP RSAS
Sbjct: 181 DYSKGRGRGRYVDERRSRSRSR----SHSYSQTRGRGRGRSVDRFASPGRQSPSPRRSAS 240

Query: 241 PQRSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 286
           PQRSPSPRGES ERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD
Sbjct: 241 PQRSPSPRGESAERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVD 283

BLAST of Carg24507 vs. TAIR 10
Match: AT5G64200.1 (ortholog of human splicing factor SC35 )

HSP 1 Score: 249.2 bits (635), Expect = 4.2e-66
Identity = 179/303 (59.08%), Postives = 198/303 (65.35%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDI+DTYSLLVLNITFRTTADDLYPLF KYGKVVD+FIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDISDTYSLLVLNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYR---- 120
           FVRYKY DEA KAVERLDG VVDGREITVQFAKYGPNAE+I KG+V E  PKSR      
Sbjct: 61  FVRYKYKDEAHKAVERLDGRVVDGREITVQFAKYGPNAEKISKGRVVEPPPKSRRSRSRS 120

Query: 121 -------------------SRSRSPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRG 180
                               RSRSPRRR RDD+R++DYR+RSRSRS         +DRR 
Sbjct: 121 PRRSRSPRRSRSPPRRRSPRRSRSPRRRSRDDYREKDYRKRSRSRS---------YDRRE 180

Query: 181 RERERERDYRRRSRSISPRRPSPDYSKGRGRGRYIDERRSRSRSRS-----RSHSHSHSQ 240
           R  E++RD+RRR+RS   R  SPD  K R RGRY +E RS SRS S     RS   S  Q
Sbjct: 181 RHEEKDRDHRRRTRS---RSASPD-EKRRVRGRYDNESRSHSRSLSASPARRSPRSSSPQ 240

Query: 241 SRGRGRGRSVDSASPGRQSPSPRSASPQRSPSPRGESPERNGDGGRSPTSRSVSPRGQPA 276
                R  S D  S   +SPSPR +   RSP+ +  SP +     R    RS SP G PA
Sbjct: 241 KTSPAREVSPDKRS-NERSPSPRRSLSPRSPALQKASPSKEMSPERRSNERSPSP-GSPA 288

BLAST of Carg24507 vs. TAIR 10
Match: AT5G64200.2 (ortholog of human splicing factor SC35 )

HSP 1 Score: 249.2 bits (635), Expect = 4.2e-66
Identity = 179/303 (59.08%), Postives = 198/303 (65.35%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDI+DTYSLLVLNITFRTTADDLYPLF KYGKVVD+FIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDISDTYSLLVLNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYR---- 120
           FVRYKY DEA KAVERLDG VVDGREITVQFAKYGPNAE+I KG+V E  PKSR      
Sbjct: 61  FVRYKYKDEAHKAVERLDGRVVDGREITVQFAKYGPNAEKISKGRVVEPPPKSRRSRSRS 120

Query: 121 -------------------SRSRSPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRG 180
                               RSRSPRRR RDD+R++DYR+RSRSRS         +DRR 
Sbjct: 121 PRRSRSPRRSRSPPRRRSPRRSRSPRRRSRDDYREKDYRKRSRSRS---------YDRRE 180

Query: 181 RERERERDYRRRSRSISPRRPSPDYSKGRGRGRYIDERRSRSRSRS-----RSHSHSHSQ 240
           R  E++RD+RRR+RS   R  SPD  K R RGRY +E RS SRS S     RS   S  Q
Sbjct: 181 RHEEKDRDHRRRTRS---RSASPD-EKRRVRGRYDNESRSHSRSLSASPARRSPRSSSPQ 240

Query: 241 SRGRGRGRSVDSASPGRQSPSPRSASPQRSPSPRGESPERNGDGGRSPTSRSVSPRGQPA 276
                R  S D  S   +SPSPR +   RSP+ +  SP +     R    RS SP G PA
Sbjct: 241 KTSPAREVSPDKRS-NERSPSPRRSLSPRSPALQKASPSKEMSPERRSNERSPSP-GSPA 288

BLAST of Carg24507 vs. TAIR 10
Match: AT1G55310.3 (SC35-like splicing factor 33 )

HSP 1 Score: 71.2 bits (173), Expect = 1.6e-12
Identity = 104/289 (35.99%), Postives = 133/289 (46.02%), Query Frame = 0

Query: 17  SLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTG------------DSRGFAFVRY 76
           SLLV N+      +DL   F ++G V DI++PRD  TG            D RGF FV++
Sbjct: 37  SLLVRNLRHDCRQEDLRKSFEQFGPVKDIYLPRDYYTGSHLCTDTCKASRDPRGFGFVQF 96

Query: 77  KYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSRSPRR 136
               +A  A   +DG ++ GRE+TV FA+   N ++  + +  E    SR+R R R+P R
Sbjct: 97  MDPADAADAKHHMDGYLLLGRELTVVFAE--ENRKKPTEMRARERGGGSRFRDRRRTPPR 156

Query: 137 RHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDY------RRRSRSISPRRP 196
            +                SRSRS       RRGR R R  DY      R   RSISPR  
Sbjct: 157 YY----------------SRSRS----PPPRRGRSRSRSGDYYSPPPRRHHPRSISPREE 216

Query: 197 SPDYSKGRGRGRYIDERRSRS----RSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSP 256
             D  +   R    D  R RS    R +SRS S S  +S  R   RS   +    +S SP
Sbjct: 217 RYDGRRSYSRSPASDGSRGRSLTPVRGKSRSLSPSPRRSISRSPRRSRSPSPKRNRSVSP 276

Query: 257 R---SASPQRSPSPRGESPERNGDGGRSPTSRSVSPR-GQPADSRSPSQ 280
           R   S SP+RS SPR        +  R   SRS SP  GQ  + RSPSQ
Sbjct: 277 RRSISRSPRRSRSPRRSRRSYTPEPAR---SRSQSPHGGQYDEDRSPSQ 300

BLAST of Carg24507 vs. TAIR 10
Match: AT1G55310.1 (SC35-like splicing factor 33 )

HSP 1 Score: 70.5 bits (171), Expect = 2.7e-12
Identity = 105/277 (37.91%), Postives = 129/277 (46.57%), Query Frame = 0

Query: 17  SLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVRYKYADEAQKAVER 76
           SLLV N+      +DL   F ++G V DI++PRD  TGD RGF FV++    +A  A   
Sbjct: 37  SLLVRNLRHDCRQEDLRKSFEQFGPVKDIYLPRDYYTGDPRGFGFVQFMDPADAADAKHH 96

Query: 77  LDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSRSPRRRHRDDHRDRDYR 136
           +DG ++ GRE+TV FA+                      R +    R R R   R RD R
Sbjct: 97  MDGYLLLGRELTVVFAE--------------------ENRKKPTEMRARERGGGRFRD-R 156

Query: 137 RRSRSRSRSRSLDRIDHDRRGRERERERDY------RRRSRSISPRRPSPDYSKGRGRGR 196
           RR+  R  SRS  R    RRGR R R  DY      R   RSISPR    D  +   R  
Sbjct: 157 RRTPPRYYSRS--RSPPPRRGRSRSRSGDYYSPPPRRHHPRSISPREERYDGRRSYSRSP 216

Query: 197 YIDERRSRS----RSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPR---SASPQRSP 256
             D  R RS    R +SRS S S  +S  R   RS   +    +S SPR   S SP+RS 
Sbjct: 217 ASDGSRGRSLTPVRGKSRSLSPSPRRSISRSPRRSRSPSPKRNRSVSPRRSISRSPRRSR 276

Query: 257 SPRGESPERNGDGGRSPTSRSVSPR-GQPADSRSPSQ 280
           SPR        +  R   SRS SP  GQ  + RSPSQ
Sbjct: 277 SPRRSRRSYTPEPAR---SRSQSPHGGQYDEDRSPSQ 287

BLAST of Carg24507 vs. TAIR 10
Match: AT3G13570.1 (SC35-like splicing factor 30A )

HSP 1 Score: 66.2 bits (160), Expect = 5.0e-11
Identity = 91/277 (32.85%), Postives = 128/277 (46.21%), Query Frame = 0

Query: 4   FGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVR 63
           FG S   D+    SLLV N+      +DL   F ++G V DI++PRD  TGD RGF F++
Sbjct: 27  FGGSRDSDLPT--SLLVRNLRHDCRQEDLRRPFEQFGPVKDIYLPRDYYTGDPRGFGFIQ 86

Query: 64  YKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKS-RYRSRSRSP 123
           +    +A +A  ++DG ++ GRE+TV FA+   N ++  + +  +   +S R++ R RSP
Sbjct: 87  FMDPADAAEAKHQMDGYLLLGRELTVVFAE--ENRKKPTEMRTRDRGGRSNRFQDRRRSP 146

Query: 124 RRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSPDY 183
            R  R     R  R RSRSR  +    +                R +SRS+SP+      
Sbjct: 147 PRYSRSPPPRRGRRSRSRSRGYNSPPAK----------------RHQSRSVSPQ------ 206

Query: 184 SKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQRS 243
                     D R  + RS SRS  H+ S+ R    GR    +   R+S SPR       
Sbjct: 207 ----------DRRYEKERSYSRSPPHNGSRVRSGSPGRVKSHSRSPRRSVSPRKNRSYTP 262

Query: 244 PSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQ 280
              R +SP       RSPT     PRG     RSPSQ
Sbjct: 267 EQARSQSPVPR--QSRSPTP---VPRGAQNGDRSPSQ 262

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7011405.14.1e-156100.00Serine/arginine-rich splicing factor SC35 [Cucurbita argyrosperma subsp. argyros... [more]
KAG6571679.11.5e-145100.00Serine/arginine-rich splicing factor SC35, partial [Cucurbita argyrosperma subsp... [more]
XP_022963472.14.7e-14499.30serine/arginine-rich splicing factor SC35-like isoform X2 [Cucurbita moschata][more]
XP_022963473.14.4e-14298.60serine/arginine-rich splicing factor SC35-like isoform X3 [Cucurbita moschata][more]
XP_022963471.14.4e-14298.60serine/arginine-rich splicing factor SC35-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FMG45.9e-6559.08Serine/arginine-rich splicing factor SC35 OS=Arabidopsis thaliana OX=3702 GN=SC3... [more]
P303521.7e-2742.74Serine/arginine-rich splicing factor 2 OS=Gallus gallus OX=9031 GN=SRSF2 PE=2 SV... [more]
Q06A983.7e-2742.74Serine/arginine-rich splicing factor 2 OS=Sus scrofa OX=9823 GN=SRSF2 PE=2 SV=1[more]
Q5R1W56.4e-2742.69Serine/arginine-rich splicing factor 2 OS=Pan troglodytes OX=9598 GN=SRSF2 PE=2 ... [more]
Q3MHR51.1e-2642.34Serine/arginine-rich splicing factor 2 OS=Bos taurus OX=9913 GN=SRSF2 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1HFC72.3e-14499.30serine/arginine-rich splicing factor SC35-like isoform X2 OS=Cucurbita moschata ... [more]
A0A6J1HG772.1e-14298.60serine/arginine-rich splicing factor SC35-like isoform X1 OS=Cucurbita moschata ... [more]
A0A6J1HHW92.1e-14298.60serine/arginine-rich splicing factor SC35-like isoform X3 OS=Cucurbita moschata ... [more]
A0A6J1HVK21.2e-13796.50serine/arginine-rich splicing factor SC35-like isoform X2 OS=Cucurbita maxima OX... [more]
A0A6J1HX791.1e-13595.82serine/arginine-rich splicing factor SC35-like isoform X3 OS=Cucurbita maxima OX... [more]
Match NameE-valueIdentityDescription
AT5G64200.14.2e-6659.08ortholog of human splicing factor SC35 [more]
AT5G64200.24.2e-6659.08ortholog of human splicing factor SC35 [more]
AT1G55310.31.6e-1235.99SC35-like splicing factor 33 [more]
AT1G55310.12.7e-1237.91SC35-like splicing factor 33 [more]
AT3G13570.15.0e-1132.85SC35-like splicing factor 30A [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 17..90
e-value: 2.6E-24
score: 96.8
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 18..88
e-value: 3.8E-20
score: 71.5
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 16..94
score: 18.990221
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 5..105
e-value: 9.0E-27
score: 95.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 224..247
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 201..215
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 255..283
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..283
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 145..200
NoneNo IPR availablePANTHERPTHR23147:SF168SERINE/ARGININE-RICH SPLICING FACTOR SC35-LIKEcoord: 1..284
NoneNo IPR availablePANTHERPTHR23147SERINE/ARGININE RICH SPLICING FACTORcoord: 1..284
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 9..100

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24507-RACarg24507-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016607 nuclear speck
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003676 nucleic acid binding