Carg24448 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24448
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionWAT1-related protein
LocationCarg_Chr04: 18354766 .. 18361806 (+)
RNA-Seq ExpressionCarg24448
SyntenyCarg24448
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTTTGTCATAAGCTCTTCATTTGATCATCACACCAAGCAAAAGCTTATGATGGGTATGAATTCTAACATTATGAGTAAGTTGAAGCCTTACATTCTAGTGGTGTGCTTACAATTTGGAATGGCTGGCATATATGTTATATCCATGGCTACTCTTAACGGCGGCATGAGCCGCTATGTTTTGATTGTCTACCGAAACGTTGTCGCCGCTCTCTTCCTTGCTCCCTTTGCACTAATCTTCGAAAGGTTTGATCTTGAGTTTTATAAATTCTTCGAGGTTTCGGACATGTTTGATCAACAACGATGATACTTTCCTTGCAGGAAAACAAGGCCAAAGATGACACTCCGGGTCGCTATGCAAATCATGGTCTTGGGATTTCTTGAGTACGAAATTTAGTTTTGTGATTTGTAAATATTGACGTATTAAAAATTTAAGAACTAAATTGATGTGGGTTTATTGTGATTTTTAGACCTGTTCTCGATCAGGGGTTCGGTTATTTGGGGATGTCGTATACGTCCGCGTCGTTTACTTCGGCTATCATGAACGCTGTGCCGTCTATTACCTTCATCATTGCTGTGGTCTTTCGGTAATTATTTATATTTTTTGGTGTGAAATTGTAATTAGTGAAGAGTTATGGGACTTGAGATTGTGTTGTTTTTTTTTAATGGTTTAGAATGGAGCGAGTCAATTTGAAACGAGTGCGAGGGGCTGCTAAGGTCATTGGAACCTTGGTGACCTTCGCCGGAGCTTTGGTTATGACGCTTTACAAAGGCCCAATAGTGGATTTCTTTTGGACTCGGAAAACCAACCACCTTGTTCATAGTGCCTCCGCCGCTTCCTCCGCCGCCAATCAGCACTGGGTGGCAGGAACTCTGTTCATCCTTCTCGCTTGTGTGGCTTGGTCTTGTTTCTATGTATTACAAGTAATTTATTCCATCTCTTATCAAACCTTTAACTTTTACCCTATTTTAATTTCATTTCAATTGGTTCATTTTTTGGGATGAACATTAAAAATTAAAAATTTGTCGGGTGAGATCCCACATTGATTGGGGAGAAGAACGTAGTATTATTTATAAAGGTGTGTAAACATATGTCTAGGAGACACGTTTTAAAAATCTTAAGGGGAGCCCGAAAGGGAAAGCCCAAATAGGACAATACTTGCTAATGATGAGCTTTGGCGAGCGGTGTGCCAACAAGGACATTGAGCCCTGAAAAGGGGTGGACACAAGACACTGTGTCAACGAGAACGCTGGCCCTCAAAAGGATTGAGCCCTGAAAAGGGGTGGACACAAGACACTGTGTCAACGAGAACGCTGGCCCTGAAAAGGATGTGGATTTGGAGGGTCCCACATCAATTGGAGAGGGAACGAGTGCCACACAAGACACTGTGTCAACGAGAACGTTGGCCCTGAAAAGGATGTGGATTTAGAGGGTCCCACATCAATTGGAGAGGGAACGAGTGCCAGCGAGAACGTTGGACCTCAAAAGGAGGGTGGATTGTGAGATTCCACATTGGTTGGGAGGAGAAAAAAACATTTTTTTCAAGGATGTGAAAACCTCTCCCTAGCAGACATGTTTTATTTATACATAAGTTTGATATCGGAGTCATACCTTAATCCTCGAGTGTCAAACAAAAAAGTTGTAAGCCTGGAATAGTCATAGTAGAGATCTATAGTAAAACTCTATCGAACCGATAAAATAGTTCACTTTAATAAGTGTTATACAAATAGATGACTACTCGAGGACGGATAGAATGGTACTTTATACAAAAAGTCTACATGCAACGTTTTTAGAACTTAATTTTTAAGCTAAATGTATGACTTGGAACAATTTTTAGTATAGTTTAGTCGATTACTTTTTAAAATATTACAAATTAGCTTTACAAATACTTTCTTATAAAAAATTTTTAAAAAAAAATGCTACCCACTAAAGTTCTTCTTAATATATTAATACCACAAACAAAAGTTGACCACTTTGCTTCTTTGTGCAGTCTGTGACAGTGAAGAGATACCCAGCGGAGCTATCACTTTCGACATTGATATGCATGGCTGGTGTGGTTCAAACCACAGTGATAGCAGTGGCCTTTGAGCATCGTGCTAGCTCATGGGCGGTGGGCTGGGACTCCAGGCTTCTGGCTCCTCTCTATACGGTCTGTTTTTCAACCTAATCCCTTGATATCCATGTTGAATGGGGTTGATATTAATAATTGGGGGTTGATATCAATTTGTTTAGGGAATAGTTGGCTCAGGAATTACATATTACTTCCAAGCTCTTGTAATGAAGACTAGAGGCCCTGTGTTTGTGACGGCTTTCAACCCTTTGTGTATGGTTGTTGTCACTGTCATCTCCTCCATTATTCTCGCCGAGAAGATCCATCTTGGAAGGTATCTCCTTTTTTTAAAGTTGGTAATAGCTCAAGTCCATTGTTAGCATATATTATCTTTATATTTTCTATTGCGAGATTCCCCTCAACATTTTAATATGAGTCTACTAGGATCAGGTTTCTACACCTTTGTTAGGAATATTTCGTTCCCCTTTCGAACCGATGTGGAATCTCACAATCCACTCCCTTGGAGACCCAGTTGACAATATCTGTTAACGGTGGACTTGAGTTGTTACATATGGTATCAGAGTCAGACATTGAGCGGTGTGCCAGCCAGGACACTGGACCCTCAAGATTCGTTCCTTATAAGGGTGTGGAAACTTCTCTTTAGTAGACGCGTTTTAAAACTATGAGACTGAGAGTGATACATAACAAGATTGTGAGATCTCACATCAGTTGGAGAGAGAAACTAAGTCTTCCTTATAAGGGTGTGGAAACCTCTCCTTAGTAGACACGTTTTAAATCTATGAGGTTGAATACGTAATAGGATTGTGAGATCTCATATCGGTTGGAGAGAGAAACGAAGCATTCTTTATAAGGGTGTGGAAACCTCTCCTTAGTAGACGGGTTTGAAAACTATGAGACTGATAGCGATACGTAACGGGATTGTGAGATCTCACATCTGTTGGAGAAAGAAACGACGCATTCCTTATAAGGGTGTGGAAACCTCTCCTTAATAGACGCGTTTTAAAACTGTGAGGCTGACAGCGATATATAACGGGATTATGAAATCTCACATCGGTAGGAGAGAGGAACGAAACATTTCTTATAAGGGTGTGGAAACCTCTCCTTAATAGACACGTTTTAAAATCGTGAGGCTAACAGCTATACATAACTAGCCAAAACAGACCATATCTACTGGCGATGGGCGTGAAGTGGTACACGAAATGAGTATTAAATTCGATCTATATATGAACATTAGATGACAGTCAAATCTTAAGAAAAGTATATCACAAACGTATGGAACATGAATACTTACAACTTGGAAGAAGAATATAATTCATTGAATCTCTTCTTTTTTGCAAACACCAGCGTTATTGGAGCCATTATCATAGCAATTGGCCTTTATTCAGTTGTTTGGGGCAAAAGCAAGGATTATTCCACCGCCGCTGGCCACCTTGAAAAGTCTAACGCCGCCGTGCCGGAGCTCCCAATTACGGCCTCTAAACTCCCCGCCGAACAAGATCCTAATGAACTTCATTAACATGTACTCAAATCATTCCCTTCTAATTGTCTTTGTTTTCAAACCCTTTTTTTCAAGTTTGAAATTTTGATCTCTTAATCGAAGATAAGGGTTTAATCAGTTGAGTTGCGCTCGAGTTTGTTGGATGCGTTTAAAGAATATTCTAATATCATGAATGACATAATATTGTATCGTCATCTTGTTCTTGTCGGATATAAAAGTAATAATAATAAAAATAATGTGGCATAATGGATTCTTTTTCGAGAATATTTAAAGATTGTAAAATCGAATGACTAGCCTGTGTCGTTATGATGATAGTAATGTATTACTAAAATTGAACATTGTATATGTGTATTTAATGTCACGATCGTACATTTTCAACCATGTGGCGTCATGATCTTGACACGATCATAACAAGTCTGAAGGAGAACTCAAGTCCCGTTTTCATTTCTCGCTCGGTTTTGTGGCTTCGTCGAACTTTAGGTGGGGTTATCCTTCGCCGATCAAACACAACTCGTGTGGAATCTAAGCTTGTTCGGCCACACACGACACTTGTGCATGCATGTTCAATCGTCTACGATTTTAGCTGACTTGCCTCTACAGTAGCCCAAATTATTTGATTTGACCTTGCCTCTACTCTAGACAAAGGTCTCTCCTATGCAACGTCGCATCTCATCTTATCATTTTGGTTCCCAATAGTATGATTCTCGATGTCATGATGTCATCCCAAGTCCTAAGATGTCATCAGCCTACTCAAACATGCAAGAACTTACCCATGTGTCTTTATATCTATTGATTTAGGCTCTATTTAGACATGGACCCTCCCATATCCATGCTAAGTTATTTACGGACTCTGTACTAGATAGCGGGTGCATATATCAACCTCCAACATATGGGCAGGGGTGCAATGTCGACACATCTGTACCATCTAACACTATCCTTGAAAAACCCATTTGAAGGAAGACGCCTAAAACACTCATCTCGCAACGCTCGCTCACATTATAACACACTATGTGCCACAAGTTAGCTCGCTTAGATCACAGGACTAGAGGTGGCAGAGAGTTGATATCATGTCTTCTCCTATAGTACGTTTTGAGACATCTTTGATTCTTATGGAATGATGCTGGACTTGTGGAGCATCGGTCCGATGTAATCTCTCGTTCAAAGCACAAAAAGTATATGCTTTCAATGAATTAAAAAGCTCAACACAAATGGGCAAAGAATCAAGACATAATTAAGAACAAGTTCCCCAAAAAATGAAACAAATTCAATGAAAGAGAACAGAATATTAGTACTAGGTTACATCAATTCCCAACAAGTGTATTATTTCCATTGTTCTATAAGTACTCCCCCATGTTCTATATCCGACAACAACAAGAACAACAACAAGATGGAAATTTGTGGAAGAATTTGGAGAATGATGAAAATAAGGAAGGCTTATTTTGCTGTCGTGTTCCTGCAGGCTTCAATTGCAGTTATGTACACCATTGTCGCTATTTCTCTTAAGCATGGCCTTAACCATTTTGTGTTTTCTGTATATCGCCATGCTGTCGCCACCGTCGTTCTCGCCCCCTTCGCTCTCATTCTCGAAAGGTCGATTTCTAGCTCTTTCTCTATAGAGAAGGTCATTACGATAACGTGACTGACTGTTTTACACCGTTAGATATACCTCGAGTTTGTAGGTTATAATAAATGTGTCGACCTTTATTGAATAATTTTTCGATTTTCGAGCATGAAAGCCGTTCTATATTTTAACTATTGTTTTTCGTATGATAGGAAAACGAGACCTCAAATGACACTACCCGTCTTCTGGAGAATTTTGTTGCTCGGTTTTCTAGAGTAATTCTCTCTTTTCTCTTTTCGTTTCTTTGTCTTCTCTTTTCCGTGGTAAAAGTTTAGAATACAGTTTTCGTGCAATTTAAACATAATTCGACTCGTTTTAGTTAACTGAGTGTACGTTTTACACAGATAAAGATGATTGTAAAATTTTGTAGGTTCTCGAGTGGATAAGAACCTATTTAATAATGATTTGAAAGGTTTTAATGCAACAGTCAATCTAAGCATAATTCAGCTGGTTAAGACATACATGATAAACAAATTCAGGCCAGTTCTCGACCAGAATTTATACTATTTGGGAATGAAGTACACATCGTCAACATTTGCATCGGCCGCCATCAACATCCTTCCGGCCATAACCTTCATCCTGGCCGTCATTTTCAGGTATACCCTATCGTACCTAAAGCCCTAATACAATTCAACTACTTAAATCTTTGAGCCTCTTTCTTTAATTTCTATTTGTGGGTGTGTGAGTGCGATCCATCTTACTTCAGCTCCTACCATAACAATTAGTATCATAGTATTTTGGTTGTGGCATTCCACTGCAGCATCAAAGCTGGGCACAACAAGTGGTATCAGAGCAGACATTAATCATAACCCTATTCTTAAATAGGCTGGAGATCGTCAACATGAAGAAAAAACATGGCTTAGCGAAGACAGTCGGAACTGTAATAGCGATCACCGGAGCCATGGTTATGAGCCTGTACAAAGGGCCGGCTTTCAACTTCCTTCCGAGCACTGGCCGTAGCGAAATGAGCCACACCGAAACGGCGACAGACCGGCACTGGGTCTCCGGCAGCATCTTCGTCTTTGTCAGTTGCTTCTGCGCGTCCGCCTTTTATATCTTACAGGTAAACAAAAGGGCTGACATGAATTCTCTGTCTCTTTAAGCTGTGGGTTCAAAATTTGGGTATTAATTGTTTGTTGGTGTGGGCAGTCCATGACATTGAAAATGTACCCTGCCGGGATTTCGCTGGCAGCTTTGATATGCCTGACCGGAACCGTGGAAGGCGGCGCCGTCACTCTCGCCGCCGAACGTCACATGATGGTCTGGATTGTCGGCTGGGATTGCAGACTTCTAGCCATCCTTTACTCCGTAATTTACCTCATCCCTAATTTCTTTTTAAGTTAAATTAATAAAAATATTTTTAAAAGGCTTGTCAGAGGTGGTGAGCTCTCATGACTTTACTTTGAACTTGCCTCATACTAATAGAGATAATATTCTTTAATTATAAACTCAGTATTTATTAAACAAAAAAAATTAAAAATTAATGAAATTATTAAATTTCACACAAAAATAAGATGTAAATTGAAAGAAAAAAAAGTTACTGATGAAAGAGAGGTAACAGGGAATAGTATGCTCGGGAATTGCATATTACGTTCAAGGAGCAGTGATGAAGGAACGCGGCCCGGTTTTTGTGACGTCATTCAGCCCTCTCTGTACGGTCATCACGGCGGCGCTCAGCTCCATTTTCTTGGCCGACAAGATTCATCTCGGAAGGTATTAACACTTTTCATGTTTGTTTCGTTTGTGTTCTTCTTTATAACGATGCGTTTATGGTTGCAGTTTGATTGGGGCAGTGATCGTGATGTTTGGATTGTATACTTTGATTTGGGGCACAAGTATAGATTTTGA

mRNA sequence

ATGAAGTTTGTCATAAGCTCTTCATTTGATCATCACACCAAGCAAAAGCTTATGATGGGTATGAATTCTAACATTATGAGTAAGTTGAAGCCTTACATTCTAGTGGTGAAAACAAGGCCAAAGATGACACTCCGGGTCGCTATGCAAATCATGGTCTTGGGATTTCTTGAACCTGTTCTCGATCAGGGGTTCGGTTATTTGGGGATGTCGTATACGTCCGCGTCGTTTACTTCGGCTATCATGAACGCTGTGCCGTCTATTACCTTCATCATTGCTGTGGTCTTTCGAATGGAGCGAGTCAATTTGAAACGAGTGCGAGGGGCTGCTAAGGTCATTGGAACCTTGGTGACCTTCGCCGGAGCTTTGGTTATGACGCTTTACAAAGGCCCAATAGTGGATTTCTTTTGGACTCGGAAAACCAACCACCTTGTTCATAGTGCCTCCGCCGCTTCCTCCGCCGCCAATCAGCACTGGGTGGCAGGAACTCTGTTCATCCTTCTCGCTTGTGTGGCTTGGTCTTGTTTCTATGTATTACAAGGAATAGTTGGCTCAGGAATTACATATTACTTCCAAGCTCTTGTAATGAAGACTAGAGGCCCTGCTTCAATTGCAGTTATGTACACCATTGTCGCTATTTCTCTTAAGCATGGCCTTAACCATTTTGTGTTTTCTGTATATCGCCATGCTGTCGCCACCGTCGTTCTCGCCCCCTTCGCTCTCATTCTCGAAAGGAAAACGAGACCTCAAATGACACTACCCGTCTTCTGGAGAATTTTGTTGCTCGTCGGAACTGTAATAGCGATCACCGGAGCCATGGTTATGAGCCTGTACAAAGGGCCGGCTTTCAACTTCCTTCCGAGCACTGGCCGTAGCGAAATGAGCCACACCGAAACGGCGACAGACCGGCACTGGGTCTCCGGCAGCATCTTCGTCTTTGTCAGTTGCTTCTGCGCGTCCGCCTTTTATATCTTACAGTTTGATTGGGGCAGTGATCGTGATGTTTGGATTGTATACTTTGATTTGGGGCACAAGTATAGATTTTGA

Coding sequence (CDS)

ATGAAGTTTGTCATAAGCTCTTCATTTGATCATCACACCAAGCAAAAGCTTATGATGGGTATGAATTCTAACATTATGAGTAAGTTGAAGCCTTACATTCTAGTGGTGAAAACAAGGCCAAAGATGACACTCCGGGTCGCTATGCAAATCATGGTCTTGGGATTTCTTGAACCTGTTCTCGATCAGGGGTTCGGTTATTTGGGGATGTCGTATACGTCCGCGTCGTTTACTTCGGCTATCATGAACGCTGTGCCGTCTATTACCTTCATCATTGCTGTGGTCTTTCGAATGGAGCGAGTCAATTTGAAACGAGTGCGAGGGGCTGCTAAGGTCATTGGAACCTTGGTGACCTTCGCCGGAGCTTTGGTTATGACGCTTTACAAAGGCCCAATAGTGGATTTCTTTTGGACTCGGAAAACCAACCACCTTGTTCATAGTGCCTCCGCCGCTTCCTCCGCCGCCAATCAGCACTGGGTGGCAGGAACTCTGTTCATCCTTCTCGCTTGTGTGGCTTGGTCTTGTTTCTATGTATTACAAGGAATAGTTGGCTCAGGAATTACATATTACTTCCAAGCTCTTGTAATGAAGACTAGAGGCCCTGCTTCAATTGCAGTTATGTACACCATTGTCGCTATTTCTCTTAAGCATGGCCTTAACCATTTTGTGTTTTCTGTATATCGCCATGCTGTCGCCACCGTCGTTCTCGCCCCCTTCGCTCTCATTCTCGAAAGGAAAACGAGACCTCAAATGACACTACCCGTCTTCTGGAGAATTTTGTTGCTCGTCGGAACTGTAATAGCGATCACCGGAGCCATGGTTATGAGCCTGTACAAAGGGCCGGCTTTCAACTTCCTTCCGAGCACTGGCCGTAGCGAAATGAGCCACACCGAAACGGCGACAGACCGGCACTGGGTCTCCGGCAGCATCTTCGTCTTTGTCAGTTGCTTCTGCGCGTCCGCCTTTTATATCTTACAGTTTGATTGGGGCAGTGATCGTGATGTTTGGATTGTATACTTTGATTTGGGGCACAAGTATAGATTTTGA

Protein sequence

MKFVISSSFDHHTKQKLMMGMNSNIMSKLKPYILVVKTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAASSAANQHWVAGTLFILLACVAWSCFYVLQGIVGSGITYYFQALVMKTRGPASIAVMYTIVAISLKHGLNHFVFSVYRHAVATVVLAPFALILERKTRPQMTLPVFWRILLLVGTVIAITGAMVMSLYKGPAFNFLPSTGRSEMSHTETATDRHWVSGSIFVFVSCFCASAFYILQFDWGSDRDVWIVYFDLGHKYRF
Homology
BLAST of Carg24448 vs. NCBI nr
Match: KAG7032792.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 676.0 bits (1743), Expect = 1.7e-190
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MKFVISSSFDHHTKQKLMMGMNSNIMSKLKPYILVVKTRPKMTLRVAMQIMVLGFLEPVL 60
           MKFVISSSFDHHTKQKLMMGMNSNIMSKLKPYILVVKTRPKMTLRVAMQIMVLGFLEPVL
Sbjct: 1   MKFVISSSFDHHTKQKLMMGMNSNIMSKLKPYILVVKTRPKMTLRVAMQIMVLGFLEPVL 60

Query: 61  DQGFGYLGMSYTSASFTSAIMNAVPSITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAG 120
           DQGFGYLGMSYTSASFTSAIMNAVPSITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAG
Sbjct: 61  DQGFGYLGMSYTSASFTSAIMNAVPSITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAG 120

Query: 121 ALVMTLYKGPIVDFFWTRKTNHLVHSASAASSAANQHWVAGTLFILLACVAWSCFYVLQG 180
           ALVMTLYKGPIVDFFWTRKTNHLVHSASAASSAANQHWVAGTLFILLACVAWSCFYVLQG
Sbjct: 121 ALVMTLYKGPIVDFFWTRKTNHLVHSASAASSAANQHWVAGTLFILLACVAWSCFYVLQG 180

Query: 181 IVGSGITYYFQALVMKTRGPASIAVMYTIVAISLKHGLNHFVFSVYRHAVATVVLAPFAL 240
           IVGSGITYYFQALVMKTRGPASIAVMYTIVAISLKHGLNHFVFSVYRHAVATVVLAPFAL
Sbjct: 181 IVGSGITYYFQALVMKTRGPASIAVMYTIVAISLKHGLNHFVFSVYRHAVATVVLAPFAL 240

Query: 241 ILERKTRPQMTLPVFWRILLLVGTVIAITGAMVMSLYKGPAFNFLPSTGRSEMSHTETAT 300
           ILERKTRPQMTLPVFWRILLLVGTVIAITGAMVMSLYKGPAFNFLPSTGRSEMSHTETAT
Sbjct: 241 ILERKTRPQMTLPVFWRILLLVGTVIAITGAMVMSLYKGPAFNFLPSTGRSEMSHTETAT 300

Query: 301 DRHWVSGSIFVFVSCFCASAFYILQFDWGSDRDVWIVYFDLGHKYRF 348
           DRHWVSGSIFVFVSCFCASAFYILQFDWGSDRDVWIVYFDLGHKYRF
Sbjct: 301 DRHWVSGSIFVFVSCFCASAFYILQFDWGSDRDVWIVYFDLGHKYRF 347

BLAST of Carg24448 vs. NCBI nr
Match: KAG6602087.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 302.0 bits (772), Expect = 6.7e-78
Identity = 181/285 (63.51%), Postives = 182/285 (63.86%), Query Frame = 0

Query: 19  MGMNSNIMSKLKPYILVV------------------------------------------ 78
           MGMNSNIMSKLKPYILVV                                          
Sbjct: 1   MGMNSNIMSKLKPYILVVCLQFGMAGIYVISMATLNGGMSRYVLIVYRNVVAALFLAPFA 60

Query: 79  -----KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFII 138
                KTRPKMTL VAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFII
Sbjct: 61  LIFERKTRPKMTLGVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFII 120

Query: 139 AVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAAS 198
           AVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAAS
Sbjct: 121 AVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAAS 180

Query: 199 SAANQHWVAGTLFILLACVAWSCFYVLQ-------------------------------- 204
           SAANQHWVAGTLFILLACVAWSCFYVLQ                                
Sbjct: 181 SAANQHWVAGTLFILLACVAWSCFYVLQSVTVKRYPAELSLSTLICMAGVVQTTVIAVAF 240

BLAST of Carg24448 vs. NCBI nr
Match: XP_022991057.1 (WAT1-related protein At4g08290-like [Cucurbita maxima])

HSP 1 Score: 300.8 bits (769), Expect = 1.5e-77
Identity = 180/286 (62.94%), Postives = 182/286 (63.64%), Query Frame = 0

Query: 18  MMGMNSNIMSKLKPYILVV----------------------------------------- 77
           MMGMNSNIMSKLKPYILVV                                         
Sbjct: 1   MMGMNSNIMSKLKPYILVVCLQFGMAGIYVISMATLNHGMSRYVLIVYRNVVAALFLAPF 60

Query: 78  ------KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFI 137
                 KTRPKMTL VAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFI
Sbjct: 61  ALIFERKTRPKMTLPVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFI 120

Query: 138 IAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA 197
           IAVVFRMERVNLKRVRG AKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA
Sbjct: 121 IAVVFRMERVNLKRVRGVAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA 180

Query: 198 SSAANQHWVAGTLFILLACVAWSCFYVLQ------------------------------- 204
           S+AANQHWVAGTLFILLACVAWSCFYVLQ                               
Sbjct: 181 SAAANQHWVAGTLFILLACVAWSCFYVLQSVTVKRYPAELSLSTLICMAGVVQTTVIAVA 240

BLAST of Carg24448 vs. NCBI nr
Match: XP_022958257.1 (WAT1-related protein At4g08290-like [Cucurbita moschata])

HSP 1 Score: 298.1 bits (762), Expect = 9.7e-77
Identity = 179/286 (62.59%), Postives = 180/286 (62.94%), Query Frame = 0

Query: 18  MMGMNSNIMSKLKPYILVV----------------------------------------- 77
           MMGMNSN MSKLKPYILVV                                         
Sbjct: 1   MMGMNSNFMSKLKPYILVVCLQFGMAGIYVISMATLNGGMSRYVLIVYRNVVAALFLAPF 60

Query: 78  ------KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFI 137
                 KTRPKMTL VAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITF 
Sbjct: 61  ALIFERKTRPKMTLPVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFS 120

Query: 138 IAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA 197
           IAVVFRMERVNLKRVRG AKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA
Sbjct: 121 IAVVFRMERVNLKRVRGVAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA 180

Query: 198 SSAANQHWVAGTLFILLACVAWSCFYVLQ------------------------------- 204
           SSAANQHWVAGTLFILLACVAWSCFYVLQ                               
Sbjct: 181 SSAANQHWVAGTLFILLACVAWSCFYVLQSVTVKRYPAELSLSTLICMAGVVQTTVIAVA 240

BLAST of Carg24448 vs. NCBI nr
Match: XP_023549003.1 (WAT1-related protein At4g08290-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298.1 bits (762), Expect = 9.7e-77
Identity = 178/286 (62.24%), Postives = 182/286 (63.64%), Query Frame = 0

Query: 18  MMGMNSNIMSKLKPYILVV----------------------------------------- 77
           M+GMN+NIMSKLKPYILVV                                         
Sbjct: 1   MIGMNANIMSKLKPYILVVCLQFGMAGIYVISMATLNRGMSRYVLIVYRNAVAALFLAPF 60

Query: 78  ------KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFI 137
                 KTRPKMTL VAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFI
Sbjct: 61  ALIFERKTRPKMTLPVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFI 120

Query: 138 IAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA 197
           IAVVFRMERVNLKRVRG AKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA
Sbjct: 121 IAVVFRMERVNLKRVRGVAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA 180

Query: 198 SSAANQHWVAGTLFILLACVAWSCFYVLQ------------------------------- 204
           S+AANQHWVAGTLFILLACVAWSCFYVLQ                               
Sbjct: 181 SAAANQHWVAGTLFILLACVAWSCFYVLQSVTVKRYPAELSLSTLICMAGVVQTTVIAVA 240

BLAST of Carg24448 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.6e-44
Identity = 113/255 (44.31%), Postives = 139/255 (54.51%), Query Frame = 0

Query: 29  LKPYILVV--KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPS 88
           L P+ L+   K RPKMTL V  +IM LGFLEPVLDQGFGYLGM+ TSA++TSAIMN +PS
Sbjct: 56  LAPFALIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPS 115

Query: 89  ITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHS 148
           +TFIIA + RME+VN+  VR  AK+IGTLV   GALVMTLYKGP++   W+   N    +
Sbjct: 116 VTFIIAWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWS-NPNMDQQN 175

Query: 149 ASAASSAANQHWVAGTLFILLACVAWSCFYVLQ--------------------------- 208
               +S  + +WV GTL ILL CVAWS FYVLQ                           
Sbjct: 176 GHTNNSQDHNNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFA 235

Query: 209 --------------------------GIVGSGITYYFQALVMKTRGPASIA-------VM 222
                                     GIV SGITYY Q +VMKTRGP  +        ++
Sbjct: 236 VALVVERHPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMIL 295

BLAST of Carg24448 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.6e-34
Identity = 86/235 (36.60%), Postives = 123/235 (52.34%), Query Frame = 0

Query: 37  KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFIIAVVFR 96
           K RPKMT R+ +QI +LGF+EPVLDQ   Y+GM+YTSA+F SA  N +P+ITF++A++FR
Sbjct: 64  KIRPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFR 123

Query: 97  MERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFF------WTRKTNHLVHSASAA 156
           +E VN K+VR  AKV+GT++T +GAL+MTLYKGPIVDF                    A 
Sbjct: 124 LESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAG 183

Query: 157 SSAANQHWVAGTLFILLACVAWSCFYVLQ------------------------------- 213
           ++A ++HW+ GTL +L     W+ F++LQ                               
Sbjct: 184 AAAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLV 243

BLAST of Carg24448 vs. ExPASy Swiss-Prot
Match: Q501F8 (WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 8.1e-34
Identity = 85/227 (37.44%), Postives = 127/227 (55.95%), Query Frame = 0

Query: 29  LKPYILVV--KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPS 88
           + P+ L++  K RPKMT  + ++I+ LGFLEP+LDQ   Y+GM  TSA+++SA +NA+P+
Sbjct: 54  IAPFALILERKIRPKMTWPLFLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPA 113

Query: 89  ITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHS 148
           ITFI+AV+FR+E VNLK+ R  AKVIGT +T  GA+VMTLYKGP ++ F T  ++    S
Sbjct: 114 ITFIMAVIFRIETVNLKKTRSLAKVIGTAITVGGAMVMTLYKGPAIELFKTAHSSLHGGS 173

Query: 149 ASAASSAANQHWVAGTLFILLACVAWSCFYVLQ--------------------------- 201
           +  +S   +Q+WV GTL ++ +   W+ F++LQ                           
Sbjct: 174 SGTSSETTDQNWVTGTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTI 233

BLAST of Carg24448 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.7e-31
Identity = 88/278 (31.65%), Postives = 148/278 (53.24%), Query Frame = 0

Query: 37  KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFIIAVVFR 96
           K +PK+T  + MQ+ +LG L PV+DQ F Y+G+ YTS +F+ A+ N +P++TFI+AV+FR
Sbjct: 71  KAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFR 130

Query: 97  MERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTR----KTNHLVHSASAASS 156
           ME ++LK++   AK+ GT+VT AGA++MT+YKGPIV+ FWT+    + +   ++ S+ +S
Sbjct: 131 MEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNS 190

Query: 157 AANQHWVAGTLFILLACVAWSCFYVLQ--------------------------------- 216
           ++++ ++ G++ ++ A +AW+  +VLQ                                 
Sbjct: 191 SSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVM 250

Query: 217 ---------------------GIVGSGITYYFQALVMKTRGP----ASIAVMYTIVAISL 253
                                GIV S I+YY Q +VMK RGP    A   +M  IVA+  
Sbjct: 251 EHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMG 310

BLAST of Carg24448 vs. ExPASy Swiss-Prot
Match: Q9LPF1 (WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 5.4e-30
Identity = 84/243 (34.57%), Postives = 136/243 (55.97%), Query Frame = 0

Query: 29  LKPYILVV--KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPS 88
           + P+ L+   K RPKMTL +  +++ LG LEP++DQ   Y+G+  TSAS+TSA  NA+P+
Sbjct: 54  MAPFALMFERKIRPKMTLAIFWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPA 113

Query: 89  ITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHS 148
           +TFI+A++FR+E VN ++V   AKV+GT++T  GA++MTLYKGP ++     K  H    
Sbjct: 114 VTFILALIFRLETVNFRKVHSVAKVVGTVITVGGAMIMTLYKGPAIEIV---KAAHNSFH 173

Query: 149 ASAASSAANQHWVAGTLFILLACVAWSCFYVLQG---------------IVGSGITYYFQ 208
             ++S+   QHWV GT+ I+ +   W+ F++LQ                I G G      
Sbjct: 174 GGSSSTPTGQHWVLGTIAIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAI 233

Query: 209 ALVMKTRGPA---------SIAVMYTIVAISLKHGLNHFVFSV---YRHAVATVVLAPFA 243
           A ++  R P+         ++A +Y+ V  S   G+ +++ S+    R  V T   +P  
Sbjct: 234 ASLIMVRDPSAWKIGMDSGTLAAVYSGVVCS---GIAYYIQSIVIKQRGPVFTTSFSPMC 290

BLAST of Carg24448 vs. ExPASy TrEMBL
Match: A0A6J1JKM8 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111487762 PE=3 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 7.2e-78
Identity = 180/286 (62.94%), Postives = 182/286 (63.64%), Query Frame = 0

Query: 18  MMGMNSNIMSKLKPYILVV----------------------------------------- 77
           MMGMNSNIMSKLKPYILVV                                         
Sbjct: 1   MMGMNSNIMSKLKPYILVVCLQFGMAGIYVISMATLNHGMSRYVLIVYRNVVAALFLAPF 60

Query: 78  ------KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFI 137
                 KTRPKMTL VAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFI
Sbjct: 61  ALIFERKTRPKMTLPVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFI 120

Query: 138 IAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA 197
           IAVVFRMERVNLKRVRG AKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA
Sbjct: 121 IAVVFRMERVNLKRVRGVAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA 180

Query: 198 SSAANQHWVAGTLFILLACVAWSCFYVLQ------------------------------- 204
           S+AANQHWVAGTLFILLACVAWSCFYVLQ                               
Sbjct: 181 SAAANQHWVAGTLFILLACVAWSCFYVLQSVTVKRYPAELSLSTLICMAGVVQTTVIAVA 240

BLAST of Carg24448 vs. ExPASy TrEMBL
Match: A0A6J1H1C7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111459533 PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 4.7e-77
Identity = 179/286 (62.59%), Postives = 180/286 (62.94%), Query Frame = 0

Query: 18  MMGMNSNIMSKLKPYILVV----------------------------------------- 77
           MMGMNSN MSKLKPYILVV                                         
Sbjct: 1   MMGMNSNFMSKLKPYILVVCLQFGMAGIYVISMATLNGGMSRYVLIVYRNVVAALFLAPF 60

Query: 78  ------KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFI 137
                 KTRPKMTL VAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITF 
Sbjct: 61  ALIFERKTRPKMTLPVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFS 120

Query: 138 IAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA 197
           IAVVFRMERVNLKRVRG AKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA
Sbjct: 121 IAVVFRMERVNLKRVRGVAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHSASAA 180

Query: 198 SSAANQHWVAGTLFILLACVAWSCFYVLQ------------------------------- 204
           SSAANQHWVAGTLFILLACVAWSCFYVLQ                               
Sbjct: 181 SSAANQHWVAGTLFILLACVAWSCFYVLQSVTVKRYPAELSLSTLICMAGVVQTTVIAVA 240

BLAST of Carg24448 vs. ExPASy TrEMBL
Match: A0A6J1ABJ2 (uncharacterized protein LOC110416484 OS=Herrania umbratica OX=108875 GN=LOC110416484 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 5.9e-64
Identity = 183/568 (32.22%), Postives = 244/568 (42.96%), Query Frame = 0

Query: 3   FVISSSFDHHTKQKLMMGMNSNIMSK--LKPYILVV--KTRPKMTLRVAMQIMVLGFLEP 62
           ++IS +   H   + ++ +  N ++   L P+ L++  + RPKMT  + +QIM LGFLEP
Sbjct: 34  YIISMASLIHGMNRYVLIVYRNAIAALVLAPFALLLERRRRPKMTFPIFLQIMTLGFLEP 93

Query: 63  VLDQGFGYLGMSYTSASFTSAIMNAVPSITFIIAVVFRMERVNLKRVRGAAKVIGTLVTF 122
           +LDQGF YLGM +TSASFTSAIMNAVPS+TF+IA++ R+E V +K VR  AK+IGT+++ 
Sbjct: 94  ILDQGFAYLGMKFTSASFTSAIMNAVPSVTFVIAIILRLEHVKIKEVRSQAKIIGTILSL 153

Query: 123 AGALVMTLYKGPIVDFFWTRKTNHLVHSASAASSAANQHWVAGTLFILLACVAWSCFYVL 182
           +GAL+MTLYKGPI+D  W+RKT+H V S  ++ S    HW+AGTL IL+ CVAWSCFYVL
Sbjct: 154 SGALLMTLYKGPIIDLIWSRKTSHHVSSGDSSDS----HWIAGTLLILVGCVAWSCFYVL 213

Query: 183 Q-----------------------------------------------------GIVGSG 242
           Q                                                     GIV SG
Sbjct: 214 QSITIKKYPAEISLSSLICLAGAIQSLAIALVVEHRLSGWAVGWDSRLFAPLYTGIVSSG 273

Query: 243 ITYYFQALVMKTRGPASI------------------------------------------ 302
           I YY Q LVMKTRGP  +                                          
Sbjct: 274 IAYYVQGLVMKTRGPVFVSAFNPLCMIIVAALGSAILGEQIHLGRYSDPSFQHHTKSYHF 333

Query: 303 ------------------------------------------------------------ 326
                                                                       
Sbjct: 334 FVQLHKEKIMEFISPQPKATSASTFRHFSAYKKAVSHIRVGSLRVSTQGKTEKERERNMG 393

BLAST of Carg24448 vs. ExPASy TrEMBL
Match: A0A5A7V1W1 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G00690 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 1.3e-63
Identity = 142/239 (59.41%), Postives = 163/239 (68.20%), Query Frame = 0

Query: 29  LKPYILVV--KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPS 88
           L P+ L+   KTRPKMTL VA+QIM+LGFLEPV+DQGFGYLGM+YTSASFTSAIMNAVPS
Sbjct: 59  LAPFALIFERKTRPKMTLPVALQIMILGFLEPVVDQGFGYLGMTYTSASFTSAIMNAVPS 118

Query: 89  ITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHS 148
           +TFIIAV+FR+ER+N+K+VRG AKVIGTLVTFAGAL+MTLYKGPI+DFFWT+KTNH V+S
Sbjct: 119 VTFIIAVLFRVERLNIKQVRGVAKVIGTLVTFAGALIMTLYKGPILDFFWTQKTNHHVNS 178

Query: 149 ASAASSAANQHWVAGTLFILLACVAWSCFYVLQ--------------------------- 208
            +AA+   NQHWVAGTLFILL CVAWSCFY+LQ                           
Sbjct: 179 GAAAT---NQHWVAGTLFILLGCVAWSCFYILQSVTVKKYPAELSLSALICLAGALQSTV 238

Query: 209 --------------------------GIVGSGITYYFQALVMKTRGPASIAVMYTIVAI 213
                                     GIVGSGI YYFQALVMKTRGP  +     +  I
Sbjct: 239 IAVAIERHASAWAVGWDSRLLAPLYTGIVGSGIAYYFQALVMKTRGPVFVTAFNPLCMI 294

BLAST of Carg24448 vs. ExPASy TrEMBL
Match: A0A5B6UB93 (WAT1-related protein OS=Gossypium australe OX=47621 GN=EPI10_009808 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 1.3e-63
Identity = 178/540 (32.96%), Postives = 233/540 (43.15%), Query Frame = 0

Query: 29  LKPYILVV--KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPS 88
           L P+ LV+  KTRPKMT  + +QIMVLGFLEP+LDQGF YLGM YTSASFTSAIMNAVPS
Sbjct: 62  LAPFALVLERKTRPKMTFPIFLQIMVLGFLEPILDQGFTYLGMKYTSASFTSAIMNAVPS 121

Query: 89  ITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHS 148
           +TF+IAV+FR+E + +K VR  AKV+GTLV+ +GAL+MTLYKGP++D  W+R T+H    
Sbjct: 122 VTFVIAVIFRLEHIKMKEVRSIAKVVGTLVSLSGALLMTLYKGPVIDLIWSRHTSH---- 181

Query: 149 ASAASSAANQHWVAGTLFILLACVAWSCFYVLQ--------------------------- 208
             ++  ++++HW++GTL IL+ CVAWSCFYVLQ                           
Sbjct: 182 NGSSGDSSDKHWISGTLLILVGCVAWSCFYVLQSITIKKYTAEISLSSLICLAGTIQSLA 241

Query: 209 --------------------------GIVGSGITYYFQALVMKTRGPASI---------- 268
                                     GIV SGITYY Q +VMKTRGP  +          
Sbjct: 242 IALVVEHRPSGWAVGWNSRLFAPLYSGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMII 301

Query: 269 ------------------------------------------------------------ 326
                                                                       
Sbjct: 302 VAALGSAILGEQLHLGSIIGGIVIAIGLYCVVWGKSKDSRSPSAPANVDCNQPELPISEK 361

BLAST of Carg24448 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 179.5 bits (454), Expect = 4.7e-45
Identity = 113/255 (44.31%), Postives = 139/255 (54.51%), Query Frame = 0

Query: 29  LKPYILVV--KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPS 88
           L P+ L+   K RPKMTL V  +IM LGFLEPVLDQGFGYLGM+ TSA++TSAIMN +PS
Sbjct: 56  LAPFALIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPS 115

Query: 89  ITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHS 148
           +TFIIA + RME+VN+  VR  AK+IGTLV   GALVMTLYKGP++   W+   N    +
Sbjct: 116 VTFIIAWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWS-NPNMDQQN 175

Query: 149 ASAASSAANQHWVAGTLFILLACVAWSCFYVLQ--------------------------- 208
               +S  + +WV GTL ILL CVAWS FYVLQ                           
Sbjct: 176 GHTNNSQDHNNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFA 235

Query: 209 --------------------------GIVGSGITYYFQALVMKTRGPASIA-------VM 222
                                     GIV SGITYY Q +VMKTRGP  +        ++
Sbjct: 236 VALVVERHPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMIL 295

BLAST of Carg24448 vs. TAIR 10
Match: AT4G08290.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 169.9 bits (429), Expect = 3.7e-42
Identity = 93/155 (60.00%), Postives = 112/155 (72.26%), Query Frame = 0

Query: 29  LKPYILVV--KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPS 88
           L P+ L+   K RPKMTL V  +IM LGFLEPVLDQGFGYLGM+ TSA++TSAIMN +PS
Sbjct: 56  LAPFALIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPS 115

Query: 89  ITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHS 148
           +TFIIA + RME+VN+  VR  AK+IGTLV   GALVMTLYKGP++   W+   N    +
Sbjct: 116 VTFIIAWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWS-NPNMDQQN 175

Query: 149 ASAASSAANQHWVAGTLFILLACVAWSCFYVLQGI 182
               +S  + +WV GTL ILL CVAWS FYVLQ I
Sbjct: 176 GHTNNSQDHNNWVVGTLLILLGCVAWSGFYVLQSI 209

BLAST of Carg24448 vs. TAIR 10
Match: AT1G21890.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 148.3 bits (373), Expect = 1.2e-35
Identity = 86/235 (36.60%), Postives = 123/235 (52.34%), Query Frame = 0

Query: 37  KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFIIAVVFR 96
           K RPKMT R+ +QI +LGF+EPVLDQ   Y+GM+YTSA+F SA  N +P+ITF++A++FR
Sbjct: 64  KIRPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFR 123

Query: 97  MERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFF------WTRKTNHLVHSASAA 156
           +E VN K+VR  AKV+GT++T +GAL+MTLYKGPIVDF                    A 
Sbjct: 124 LESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAG 183

Query: 157 SSAANQHWVAGTLFILLACVAWSCFYVLQ------------------------------- 213
           ++A ++HW+ GTL +L     W+ F++LQ                               
Sbjct: 184 AAAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLV 243

BLAST of Carg24448 vs. TAIR 10
Match: AT4G08300.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 146.0 bits (367), Expect = 5.7e-35
Identity = 85/227 (37.44%), Postives = 127/227 (55.95%), Query Frame = 0

Query: 29  LKPYILVV--KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPS 88
           + P+ L++  K RPKMT  + ++I+ LGFLEP+LDQ   Y+GM  TSA+++SA +NA+P+
Sbjct: 54  IAPFALILERKIRPKMTWPLFLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPA 113

Query: 89  ITFIIAVVFRMERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTRKTNHLVHS 148
           ITFI+AV+FR+E VNLK+ R  AKVIGT +T  GA+VMTLYKGP ++ F T  ++    S
Sbjct: 114 ITFIMAVIFRIETVNLKKTRSLAKVIGTAITVGGAMVMTLYKGPAIELFKTAHSSLHGGS 173

Query: 149 ASAASSAANQHWVAGTLFILLACVAWSCFYVLQ--------------------------- 201
           +  +S   +Q+WV GTL ++ +   W+ F++LQ                           
Sbjct: 174 SGTSSETTDQNWVTGTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTI 233

BLAST of Carg24448 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-32
Identity = 88/278 (31.65%), Postives = 148/278 (53.24%), Query Frame = 0

Query: 37  KTRPKMTLRVAMQIMVLGFLEPVLDQGFGYLGMSYTSASFTSAIMNAVPSITFIIAVVFR 96
           K +PK+T  + MQ+ +LG L PV+DQ F Y+G+ YTS +F+ A+ N +P++TFI+AV+FR
Sbjct: 71  KAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFR 130

Query: 97  MERVNLKRVRGAAKVIGTLVTFAGALVMTLYKGPIVDFFWTR----KTNHLVHSASAASS 156
           ME ++LK++   AK+ GT+VT AGA++MT+YKGPIV+ FWT+    + +   ++ S+ +S
Sbjct: 131 MEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNS 190

Query: 157 AANQHWVAGTLFILLACVAWSCFYVLQ--------------------------------- 216
           ++++ ++ G++ ++ A +AW+  +VLQ                                 
Sbjct: 191 SSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVM 250

Query: 217 ---------------------GIVGSGITYYFQALVMKTRGP----ASIAVMYTIVAISL 253
                                GIV S I+YY Q +VMK RGP    A   +M  IVA+  
Sbjct: 251 EHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMG 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7032792.11.7e-190100.00WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6602087.16.7e-7863.51WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022991057.11.5e-7762.94WAT1-related protein At4g08290-like [Cucurbita maxima][more]
XP_022958257.19.7e-7762.59WAT1-related protein At4g08290-like [Cucurbita moschata][more]
XP_023549003.19.7e-7762.24WAT1-related protein At4g08290-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SUF16.6e-4444.31WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2... [more]
F4HZQ71.6e-3436.60WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2... [more]
Q501F88.1e-3437.44WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2... [more]
Q9FL411.7e-3131.65WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
Q9LPF15.4e-3034.57WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1JKM87.2e-7862.94WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111487762 PE=3 SV=1[more]
A0A6J1H1C74.7e-7762.59WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111459533 PE=3 SV=1[more]
A0A6J1ABJ25.9e-6432.22uncharacterized protein LOC110416484 OS=Herrania umbratica OX=108875 GN=LOC11041... [more]
A0A5A7V1W11.3e-6359.41WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184... [more]
A0A5B6UB931.3e-6332.96WAT1-related protein OS=Gossypium australe OX=47621 GN=EPI10_009808 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G08290.14.7e-4544.31nodulin MtN21 /EamA-like transporter family protein [more]
AT4G08290.23.7e-4260.00nodulin MtN21 /EamA-like transporter family protein [more]
AT1G21890.11.2e-3536.60nodulin MtN21 /EamA-like transporter family protein [more]
AT4G08300.15.7e-3537.44nodulin MtN21 /EamA-like transporter family protein [more]
AT5G07050.11.2e-3231.65nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 33..125
e-value: 3.1E-7
score: 30.6
NoneNo IPR availablePANTHERPTHR31218:SF289WAT1-RELATED PROTEINcoord: 204..261
NoneNo IPR availablePANTHERPTHR31218:SF289WAT1-RELATED PROTEINcoord: 261..325
coord: 32..180
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 38..127
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 204..261
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 261..325
coord: 32..180

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24448-RACarg24448-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity