Carg24416 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24416
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein EI24 homolog isoform X1
Location	Carg_Chr04: 10795069 .. 10796592 (-)
RNA-Seq Expression	Carg24416
Synteny	Carg24416

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCTTTCCAGGTAGTCCTTGCGTTCTTGCGATATTTTTCTTCTCACCACTTGTGAGCTATGGAGTAATGGCTATCTTATTTCCACTGGTAAATTGAACCATTCGTCTCCCTCTCTTCACACACACAAACTCATTTCCTTTTAAATTTGTACCGATGTTCTCTTTCCTCAAATACTCACTTCTTACGGTTCTTTTGTTTGGGTTTCATCCCATACGCCTTTTAGTTTGTTCTGACAGCCACGGGCTCAGTTGCTGAGAAGGATATTTATTCACAGAGAACAACTTGGAAATGTGCAGGACTGGGAAGGCTTCCAATTTTCTATGCTGCAAATACATTGTCGTAAGTTGTCTTTCTTCACGCTAAACTTGTGTTTACAATATAGAATAATTTCTGAGACTATTCGAATAACCTTTGGATTTCTGATCCCATAATAATTTCTAGTATCTATATAGAATAGAAGTTGACTAAATGTGACAAGTAATGATGTTTTTAATGTTAAGCAAATGCTGCAAAATCTCTGGAATTGTAACGGGGAAAAACAGCAGTCTGTCTAAAAAATGGACAAAAGAAAGAAACCCATCTTCACGAAGATAATAGATAGAGGTTTATATAGTGAGGCTATGACTTTGTGCTCTGTAGTATAGGAGTACAATAAATTGTAGAGATTGAACCAACGAATGGAAAACCTGAAAAACTTCAATTATGTGATATTTTGAACTTTTCTAGATATTAGAACTGGCCGCGAAGCAGGGAAACTTTAGTATTGCCTACCAATGTTTTTTCCCCATGTTGATTGGCCTTGTAGGATGAAGCTCTTGACTCTCTTCCCTCATGAATCACAAGAAAAGATGCAGCAAAACAAGGAACTATAACAAGTTCTGGAGTCTGAATCAATACATATACGTTTCATGAACAGTAAGCCAAAGTTCATTAGCCATTGCCTTCTATTCAGAATATTTCCTGCATGTTCATTTATTTATGAAGTATTGCTGCATAATATATCGTCTCTCAATTCTTAATCTATCTGAAGGAGCAGAATCCAAGTGGCTCAAGTCAGATGCTACAAGTGATTCCTCCCTCATCCGTACAGAAACGATACCGTAGGTGAAGATCAACCATGGGTAGATTCATAACCGCAGTTTGTAAGATTGGTTGCTGTGTGAATATCGAATAGGTACGCAATTAATTGGGCGGATAGATAAGAATGTGAGATGCCGAGTGATAATTTTGCTGTAACCTCGTTAAATAATGTAAATAACTTATGTTTAGTAGAAAGATTGTTCCAACTCTCTGCTTCAGTTCTTTGTGTGAATTATGTACAATCAAGAATGAACAACTTAGAGTGGATAGAGTTGATAAGAATCCTTGACTTGATGGATTGAGTATACAATTGTTCATAAGTTGGTCGTGAGAAGAAGGTAAACTGATTCTGGGTATAATTAGAAAGCAGAAAAGGGAGTTAATTAAGCTGATTTACACCCTTCAAATCTTTTACATCTGCAGATAGATAGATATGTTGTG

mRNA sequence

ATGGTCTTTCCAGGTAGTCCTTGCGTTCTTGCGATATTTTTCTTCTCACCACTTGTGAGCTATGGAGTAATGGCTATCTTATTTCCACTGTTTGTTCTGACAGCCACGGGCTCAGTTGCTGAGAAGGATATTTATTCACAGAGAACAACTTGGAAATGTGCAGGACTGGGAAGGCTTCCAATTTTCTATGCTGCAAATACATTGTCGATGAAGCTCTTGACTCTCTTCCCTCATGAATCACAAGAAAAGATGCAGCAAAACAAGGAACTATAACAAGTTCTGGAGTCTGAATCAATACATATACGTTTCATGAACAAATCCAAGTGGCTCAAGTCAGATGCTACAAGTGATTCCTCCCTCATCCGTACAGAAACGATACCGTAGGTGAAGATCAACCATGGGTAGATTCATAACCGCAGTTTGTAAGATTGGTTGCTGTGTGAATATCGAATAGGTACGCAATTAATTGGGCGGATAGATAAGAATGTGAGATGCCGAGTGATAATTTTGCTGTAACCTCGTTAAATAATGTAAATAACTTATGTTTAGTAGAAAGATTGTTCCAACTCTCTGCTTCAGTTCTTTGTGTGAATTATGTACAATCAAGAATGAACAACTTAGAGTGGATAGAGTTGATAAGAATCCTTGACTTGATGGATTGAGTATACAATTGTTCATAAGTTGGTCGTGAGAAGAAGGTAAACTGATTCTGGGTATAATTAGAAAGCAGAAAAGGGAGTTAATTAAGCTGATTTACACCCTTCAAATCTTTTACATCTGCAGATAGATAGATATGTTGTG

Coding sequence (CDS)

ATGGTCTTTCCAGGTAGTCCTTGCGTTCTTGCGATATTTTTCTTCTCACCACTTGTGAGCTATGGAGTAATGGCTATCTTATTTCCACTGTTTGTTCTGACAGCCACGGGCTCAGTTGCTGAGAAGGATATTTATTCACAGAGAACAACTTGGAAATGTGCAGGACTGGGAAGGCTTCCAATTTTCTATGCTGCAAATACATTGTCGATGAAGCTCTTGACTCTCTTCCCTCATGAATCACAAGAAAAGATGCAGCAAAACAAGGAACTATAA

Protein sequence

MVFPGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYAANTLSMKLLTLFPHESQEKMQQNKEL

Homology

[Close]

BLAST of Carg24416 vs. NCBI nr
Match: KAG7032380.1 (Protein EI24-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 178.3 bits (451), Expect = 2.9e-41
Identity = 90/90 (100.00%), Postives = 90/90 (100.00%), Query Frame = 0


Query: 1  MVFPGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLP 60
          MVFPGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLP
Sbjct: 1  MVFPGSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLP 60

Query: 61 IFYAANTLSMKLLTLFPHESQEKMQQNKEL 91
          IFYAANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 61 IFYAANTLSMKLLTLFPHESQEKMQQNKEL 90

[Close]

BLAST of Carg24416 vs. NCBI nr
Match: KAG6601621.1 (Protein EI24-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 170.6 bits (431), Expect = 6.0e-39
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 64
           GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA
Sbjct: 384 GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 443

Query: 65  ANTLSMKLLTLFPHESQEKMQQNKEL 91
           ANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 444 ANTLSMKLLTLFPHESQEKMQQNKEL 469

[Close]

BLAST of Carg24416 vs. NCBI nr
Match: XP_022998222.1 (protein EI24 homolog isoform X1 [Cucurbita maxima] >XP_022998232.1 protein EI24 homolog isoform X1 [Cucurbita maxima])

HSP 1 Score: 170.6 bits (431), Expect = 6.0e-39
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 64
           GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA
Sbjct: 252 GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 311

Query: 65  ANTLSMKLLTLFPHESQEKMQQNKEL 91
           ANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 312 ANTLSMKLLTLFPHESQEKMQQNKEL 337

[Close]

BLAST of Carg24416 vs. NCBI nr
Match: XP_022957442.1 (protein EI24 homolog isoform X1 [Cucurbita moschata] >XP_022957443.1 protein EI24 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 170.6 bits (431), Expect = 6.0e-39
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 64
           GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA
Sbjct: 252 GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 311

Query: 65  ANTLSMKLLTLFPHESQEKMQQNKEL 91
           ANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 312 ANTLSMKLLTLFPHESQEKMQQNKEL 337

[Close]

BLAST of Carg24416 vs. NCBI nr
Match: XP_023515037.1 (protein EI24 homolog [Cucurbita pepo subsp. pepo] >XP_023515046.1 protein EI24 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 169.1 bits (427), Expect = 1.8e-38
Identity = 85/86 (98.84%), Postives = 85/86 (98.84%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 64
           GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGR PIFYA
Sbjct: 252 GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRFPIFYA 311

Query: 65  ANTLSMKLLTLFPHESQEKMQQNKEL 91
           ANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 312 ANTLSMKLLTLFPHESQEKMQQNKEL 337

[Close]

BLAST of Carg24416 vs. ExPASy Swiss-Prot
Match: Q5BPL5 (Protein EI24 homolog OS=Arabidopsis thaliana OX=3702 GN=At4g06676 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.8e-22
Identity = 57/80 (71.25%), Postives = 64/80 (80.00%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 64
           GSPCVLAIFF SPLVS  +MAILFPLFVLTATGS  EK I + R TWKCAGLG+LPIFY 
Sbjct: 241 GSPCVLAIFFLSPLVSGALMAILFPLFVLTATGSGPEKLIGAPRRTWKCAGLGKLPIFYI 300

Query: 65  ANTLSMKLLTLF----PHES 81
           A+TLSM  L++F    PHE+
Sbjct: 301 ADTLSMLALSIFRLESPHEN 320

[Close]

BLAST of Carg24416 vs. ExPASy TrEMBL
Match: A0A6J1K7C5 (protein EI24 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492932 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.9e-39
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 64
           GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA
Sbjct: 252 GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 311

Query: 65  ANTLSMKLLTLFPHESQEKMQQNKEL 91
           ANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 312 ANTLSMKLLTLFPHESQEKMQQNKEL 337

[Close]

BLAST of Carg24416 vs. ExPASy TrEMBL
Match: A0A6J1H083 (protein EI24 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458837 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.9e-39
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 64
           GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA
Sbjct: 252 GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 311

Query: 65  ANTLSMKLLTLFPHESQEKMQQNKEL 91
           ANTLSMKLLTLFPHESQEKMQQNKEL
Sbjct: 312 ANTLSMKLLTLFPHESQEKMQQNKEL 337

[Close]

BLAST of Carg24416 vs. ExPASy TrEMBL
Match: A0A5A7SXH4 (Protein EI24-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002620 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 7.2e-38
Identity = 83/86 (96.51%), Postives = 85/86 (98.84%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 64
           GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAE+DIYSQR TWKCAGLGRLPIFYA
Sbjct: 133 GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEQDIYSQRATWKCAGLGRLPIFYA 192

Query: 65  ANTLSMKLLTLFPHESQEKMQQNKEL 91
           ANTLSMKLLTLFPHESQEKMQQNK+L
Sbjct: 193 ANTLSMKLLTLFPHESQEKMQQNKDL 218

[Close]

BLAST of Carg24416 vs. ExPASy TrEMBL
Match: A0A1S3BE01 (protein EI24 homolog OS=Cucumis melo OX=3656 GN=LOC103488854 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 7.2e-38
Identity = 83/86 (96.51%), Postives = 85/86 (98.84%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 64
           GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAE+DIYSQR TWKCAGLGRLPIFYA
Sbjct: 252 GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEQDIYSQRATWKCAGLGRLPIFYA 311

Query: 65  ANTLSMKLLTLFPHESQEKMQQNKEL 91
           ANTLSMKLLTLFPHESQEKMQQNK+L
Sbjct: 312 ANTLSMKLLTLFPHESQEKMQQNKDL 337

[Close]

BLAST of Carg24416 vs. ExPASy TrEMBL
Match: A0A0A0KPW5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G587090 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.4e-36
Identity = 81/86 (94.19%), Postives = 84/86 (97.67%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 64
           GSPCVLAIFFFSPLVSY VMAILFPLFVLTATGSVAE+DIYSQR TWKCAGLGRLPIFYA
Sbjct: 294 GSPCVLAIFFFSPLVSYAVMAILFPLFVLTATGSVAEQDIYSQRATWKCAGLGRLPIFYA 353

Query: 65  ANTLSMKLLTLFPHESQEKMQQNKEL 91
           ANTLSMKLLTLFPHESQEKMQQ+K+L
Sbjct: 354 ANTLSMKLLTLFPHESQEKMQQSKDL 379

[Close]

BLAST of Carg24416 vs. TAIR 10
Match: AT4G06676.1 (CONTAINS InterPro DOMAIN/s: Etoposide-induced 2.4 (InterPro:IPR009890); Has 264 Blast hits to 262 proteins in 100 species: Archae - 0; Bacteria - 0; Metazoa - 130; Fungi - 16; Plants - 79; Viruses - 0; Other Eukaryotes - 39 (source: NCBI BLink). )

HSP 1 Score: 106.3 bits (264), Expect = 1.3e-23
Identity = 57/80 (71.25%), Postives = 64/80 (80.00%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFVLTATGSVAEKDIYSQRTTWKCAGLGRLPIFYA 64
           GSPCVLAIFF SPLVS  +MAILFPLFVLTATGS  EK I + R TWKCAGLG+LPIFY 
Sbjct: 241 GSPCVLAIFFLSPLVSGALMAILFPLFVLTATGSGPEKLIGAPRRTWKCAGLGKLPIFYI 300

Query: 65  ANTLSMKLLTLF----PHES 81
           A+TLSM  L++F    PHE+
Sbjct: 301 ADTLSMLALSIFRLESPHEN 320

[Close]

BLAST of Carg24416 vs. TAIR 10
Match: AT4G06676.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; CONTAINS InterPro DOMAIN/s: Etoposide-induced 2.4 (InterPro:IPR009890); Has 261 Blast hits to 259 proteins in 97 species: Archae - 0; Bacteria - 0; Metazoa - 130; Fungi - 16; Plants - 81; Viruses - 0; Other Eukaryotes - 34 (source: NCBI BLink). )

HSP 1 Score: 43.1 bits (100), Expect = 1.4e-04
Identity = 23/28 (82.14%), Postives = 24/28 (85.71%), Query Frame = 0


Query: 5   GSPCVLAIFFFSPLVSYGVMAILFPLFV 33
           GSPCVLAIFF SPLVS  +MAILFPL V
Sbjct: 241 GSPCVLAIFFLSPLVSGALMAILFPLNV 268

The following BLAST results are available for this feature:

BLAST of Carg24416 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7032380.1	2.9e-41	100.00	Protein EI24-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6601621.1	6.0e-39	100.00	Protein EI24-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022998222.1	6.0e-39	100.00	protein EI24 homolog isoform X1 [Cucurbita maxima] >XP_022998232.1 protein EI24 ...	[more]
XP_022957442.1	6.0e-39	100.00	protein EI24 homolog isoform X1 [Cucurbita moschata] >XP_022957443.1 protein EI2...	[more]
XP_023515037.1	1.8e-38	98.84	protein EI24 homolog [Cucurbita pepo subsp. pepo] >XP_023515046.1 protein EI24 h...	[more]

BLAST of Carg24416 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5BPL5	1.8e-22	71.25	Protein EI24 homolog OS=Arabidopsis thaliana OX=3702 GN=At4g06676 PE=2 SV=1	[more]

BLAST of Carg24416 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K7C5	2.9e-39	100.00	protein EI24 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492932 PE=4...	[more]
A0A6J1H083	2.9e-39	100.00	protein EI24 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458837 PE...	[more]
A0A5A7SXH4	7.2e-38	96.51	Protein EI24-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3BE01	7.2e-38	96.51	protein EI24 homolog OS=Cucumis melo OX=3656 GN=LOC103488854 PE=4 SV=1	[more]
A0A0A0KPW5	1.4e-36	94.19	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G587090 PE=4 SV=1	[more]

BLAST of Carg24416 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G06676.1	1.3e-23	71.25	CONTAINS InterPro DOMAIN/s: Etoposide-induced 2.4 (InterPro:IPR009890); Has 264 ...	[more]
AT4G06676.2	1.4e-04	82.14	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009890	Etoposide-induced 2.4	PANTHER	PTHR21389	P53 INDUCED PROTEIN	coord: 5..86

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24416-RA	Carg24416-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane