Carg24372 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24372
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase family protein
Location	Carg_Chr07: 5486465 .. 5488164 (+)
RNA-Seq Expression	Carg24372
Synteny	Carg24372

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACATTTCGTCCACATTTAGTCCAATCAATGAAGGTCTCCATTGTTTCCAGGAGTGGTAGAGAAAATCATCGCTGCCGGCCTTCAGATCGATGATTCGGTGAGCTTCTCGTTCTTTGTTATTGTAGTCGGAAGCTCTCCTGGTTAATGAGATTATGCGGACTTTGCTGTGTTTCTTTACGTGGAGCCTGTTGTTCTGATGTATCTTTGTGCGATAGAGTCGAAAATCGGAATCTCACTGTTTTATTGCTTCTTTATTTCTGAATTTGTGTCTACTTAATTTACTTCTGTATGAAGATTTGTAGACTACGGTGAAAGACTTGCAGGAGACCATTCATCAGCGACGCAAGTCTTGACTCCACTATTTTTGTTTCTGTCATCAGTTTCTGGTTGATTAAGCTTTAGATTTAGCGCTTCTGCTACAATCATTGATTTTATCGACTGCTTGCTCTGTCATATTGAAAATCCATCTCAATAGGAATGATAACGGAAGTTCTAGTGCAGCAAGGTCTTACTATTCATAATTACGCGTTCGATGAGATAAAAAGGAGTTTAGTTCTAACTTCTGATCATGTGTTGCAGTAGCTTTCTATTGTCTTTCTGGATTGCTAAAGCATGAATTGATTGATACAATACTAGCTGGCAATTCCTCATGTATTTTGTTATATCTTTCAATTTTCCCACTTAATCTCTTCCAAACACGATAAACGAGAGATTAATGTCTCCCAAACTCAGCAAACTGATTGTCTTCTTATATTCTCCCATTCAACAGGAAGAAAATACTGTTCCTCTAGACAGCGTCTTACCCTGTCTGCCAAACCAGGGTCAAAAGAGTCCTATTGTTCTAATCTCAAGCAAGAAACTCCAAGATTTGCGATTACCATCACATTTAAGGACGTTGGTCCCTGTTGAAGGATCACTAGTAGATACATATATGAATAGATACATGTGTTGAAGGAACACCTATAGATACATGTGTTAAAGGGACACCCGTAGATACATGTGTTAAAAGATCACTTGTACATGTATAAGGACATGTATATATACATGTAGGTTTATTAGTTCCTAATTAATTAAGGGCATAATGAATGGTTCTTGGAATGTTCCAAATTACCTCTATATATATTATTGGCATCGGTGGAAGATGTAAAAAATCAATTAAGCATAGTTATACATTTCTCTGTTGTCACCGAGTCTCCAAGTTTCTTATAGAACCACTTGTTATCTCTATCCCACCCAGTCTTAAGTTTTTTTTTGACTAAATAGAGGAGCGTCATACCTACCCTGTTCAGACGTATGCCATGTACTATTATTTCCTCCATTAGTCTAAGCGGATCATGTAGAACTTCTTGTTCATAGGCTTAGTCATACAGCTTCACCCTTGTCAAGTGTCTTCCGTAACTACACGTATCATTGGAGTTTTGGAGCTCTCATAGCGTACTACATAAACCACCCACTTTACACCCTGGTGTATGACCGCCAAATGAAGATTGGTTTTGGATTTTGATTACTCTGTCAAATCTTTAACTTATATCACCATCTCATATTGAAGAGTCTTAGAGACCGTAGTGGAAAGGGAGGCTACAAAATTCCACGTGGGTTTCCTTTCCTTTCATGTTGTAACTTATGCCAACTACACAACTGAGATTCACCAATGGCTGGGATTCAATATCGCAACACAAACTGTGCTGGTTATATTTTTG

mRNA sequence

ACATTTCGTCCACATTTAGTCCAATCAATGAAGGTCTCCATTGTTTCCAGGAGTGGTAGAGAAAATCATCGCTGCCGGCCTTCAGATCGATGATTCGAGGAGCGTCATACCTACCCTGTTCAGACCGGATCATGTAGAACTTCTTGTTCATAGGCTTAGTCATACAGCTTCACCCTTGTCAAGTGTCTTCCGTAACTACACGTATCATTGGAGTTTTGGAGCTCTCATAGCGTACTACATAAACCACCCACTTTACACCCTGGTGTATGACCGCCAAATGAAGATTGAGACCGTAGTGGAAAGGGAGGCTACAAAATTCCACGTGGGTTTCCTTTCCTTTCATGTTGTAACTTATGCCAACTACACAACTGAGATTCACCAATGGCTGGGATTCAATATCGCAACACAAACTGTGCTGGTTATATTTTTG

Coding sequence (CDS)

ATGAAGATTGAGACCGTAGTGGAAAGGGAGGCTACAAAATTCCACGTGGGTTTCCTTTCCTTTCATGTTGTAACTTATGCCAACTACACAACTGAGATTCACCAATGGCTGGGATTCAATATCGCAACACAAACTGTGCTGGTTATATTTTTG

Protein sequence

MKIETVVEREATKFHVGFLSFHVVTYANYTTEIHQWLGFNIATQTVLVIFL

Homology

[Close]

BLAST of Carg24372 vs. NCBI nr
Match: KAG7027329.1 (Very-long-chain enoyl-CoA reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 103.6 bits (257), Expect = 5.1e-19
Identity = 51/51 (100.00%), Postives = 51/51 (100.00%), Query Frame = 0


Query: 1  MKIETVVEREATKFHVGFLSFHVVTYANYTTEIHQWLGFNIATQTVLVIFL 52
          MKIETVVEREATKFHVGFLSFHVVTYANYTTEIHQWLGFNIATQTVLVIFL
Sbjct: 1  MKIETVVEREATKFHVGFLSFHVVTYANYTTEIHQWLGFNIATQTVLVIFL 51

[Close]

BLAST of Carg24372 vs. ExPASy Swiss-Prot
Match: Q9M2U2 (Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 5.2e-06
Identity = 24/30 (80.00%), Postives = 28/30 (93.33%), Query Frame = 0


Query: 17  GFLSFHVVTYANYTTEIHQWLGFNIATQTV 47
           GFL F++VT ANYTTEI+QWLGFNIATQT+
Sbjct: 234 GFL-FNIVTCANYTTEIYQWLGFNIATQTI 262

[Close]

BLAST of Carg24372 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 50.8 bits (120), Expect = 3.7e-07
Identity = 24/30 (80.00%), Postives = 28/30 (93.33%), Query Frame = 0


Query: 17  GFLSFHVVTYANYTTEIHQWLGFNIATQTV 47
           GFL F++VT ANYTTEI+QWLGFNIATQT+
Sbjct: 234 GFL-FNIVTCANYTTEIYQWLGFNIATQTI 262

The following BLAST results are available for this feature:

BLAST of Carg24372 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027329.1	5.1e-19	100.00	Very-long-chain enoyl-CoA reductase, partial [Cucurbita argyrosperma subsp. argy...	[more]

BLAST of Carg24372 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2U2	5.2e-06	80.00	Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 ...	[more]

BLAST of Carg24372 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Carg24372 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55360.1	3.7e-07	80.00	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 21..51 score: 9.013925

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24372-RA	Carg24372-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors