Carg24324 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24324
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	VQ domain-containing protein
Location	Carg_Chr20: 10061858 .. 10062493 (+)
RNA-Seq Expression	Carg24324
Synteny	Carg24324

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACCCGCCGGGATATCCGGCCGGCGGCAGCCCCAGAAAGAAGGAGATCCAGCTACAGGGTCCTCGCCCGCCGCAACTCCGCGTCAATCAAGATTCCCGCAAAATCAAGAAGCCGCCGCCACACCCTCAGCCGATTCCCCCCGCCGGACGGCCACCTCTCCCGCCCACCCAATGGCCTCAGCCACTCATCATCTACGACATCTCCCCCAAAGTCATCCACGTCCCAGAGAACAACTTCATGTCCGTCGTGCAACGACTCACTGGACTCTCTTCCTCCGGCGCCGCCGCCCCCGACGGCGATCTCTCCCCAGCGGCGAGGCTGGCCGCAATCGAGAAGGCCAGTCCCCGACCGGAAAGAGAAAGAGAAAGAGAAATCGACGTTAGCGAGATGATGGATTTGGGGGAGATTTCGGTGGAGTTATGGCAAACCCCAGGAATCCTATCTCCGGCTCCGGCGAGTCTTGCTCCGATTCCGACAGGGTTTTTCTCGCCGGCGACAGAGCTTCAAAATTTGTCGTATTCGTTAATTCAGGAGCTGAGCCCTCATTGGCCGAGCCCTTCTGCTCTGTTTTCAGCTCCTCTGGTTTCCCCAATTTCTTCACCAAATCTTTTCAACAATCTCTTTGATTTCTAG

mRNA sequence

ATGAACCCGCCGGGATATCCGGCCGGCGGCAGCCCCAGAAAGAAGGAGATCCAGCTACAGGGTCCTCGCCCGCCGCAACTCCGCGTCAATCAAGATTCCCGCAAAATCAAGAAGCCGCCGCCACACCCTCAGCCGATTCCCCCCGCCGGACGGCCACCTCTCCCGCCCACCCAATGGCCTCAGCCACTCATCATCTACGACATCTCCCCCAAAGTCATCCACGTCCCAGAGAACAACTTCATGTCCGTCGTGCAACGACTCACTGGACTCTCTTCCTCCGGCGCCGCCGCCCCCGACGGCGATCTCTCCCCAGCGGCGAGGCTGGCCGCAATCGAGAAGGCCAGTCCCCGACCGGAAAGAGAAAGAGAAAGAGAAATCGACGTTAGCGAGATGATGGATTTGGGGGAGATTTCGGTGGAGTTATGGCAAACCCCAGGAATCCTATCTCCGGCTCCGGCGAGTCTTGCTCCGATTCCGACAGGGTTTTTCTCGCCGGCGACAGAGCTTCAAAATTTGTCGTATTCGTTAATTCAGGAGCTGAGCCCTCATTGGCCGAGCCCTTCTGCTCTGTTTTCAGCTCCTCTGGTTTCCCCAATTTCTTCACCAAATCTTTTCAACAATCTCTTTGATTTCTAG

Coding sequence (CDS)

ATGAACCCGCCGGGATATCCGGCCGGCGGCAGCCCCAGAAAGAAGGAGATCCAGCTACAGGGTCCTCGCCCGCCGCAACTCCGCGTCAATCAAGATTCCCGCAAAATCAAGAAGCCGCCGCCACACCCTCAGCCGATTCCCCCCGCCGGACGGCCACCTCTCCCGCCCACCCAATGGCCTCAGCCACTCATCATCTACGACATCTCCCCCAAAGTCATCCACGTCCCAGAGAACAACTTCATGTCCGTCGTGCAACGACTCACTGGACTCTCTTCCTCCGGCGCCGCCGCCCCCGACGGCGATCTCTCCCCAGCGGCGAGGCTGGCCGCAATCGAGAAGGCCAGTCCCCGACCGGAAAGAGAAAGAGAAAGAGAAATCGACGTTAGCGAGATGATGGATTTGGGGGAGATTTCGGTGGAGTTATGGCAAACCCCAGGAATCCTATCTCCGGCTCCGGCGAGTCTTGCTCCGATTCCGACAGGGTTTTTCTCGCCGGCGACAGAGCTTCAAAATTTGTCGTATTCGTTAATTCAGGAGCTGAGCCCTCATTGGCCGAGCCCTTCTGCTCTGTTTTCAGCTCCTCTGGTTTCCCCAATTTCTTCACCAAATCTTTTCAACAATCTCTTTGATTTCTAG

Protein sequence

MNPPGYPAGGSPRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPPTQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKASPRPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYSLIQELSPHWPSPSALFSAPLVSPISSPNLFNNLFDF

Homology

[Close]

BLAST of Carg24324 vs. NCBI nr
Match: KAG6571354.1 (Protein MKS1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011126.1 Protein MKS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 414.5 bits (1064), Expect = 5.6e-112
Identity = 211/211 (100.00%), Postives = 211/211 (100.00%), Query Frame = 0


Query: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPPTQWP 60
           MNPPGYPAGGSPRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPPTQWP
Sbjct: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPPTQWP 60

Query: 61  QPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKASPRPER 120
           QPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKASPRPER
Sbjct: 61  QPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKASPRPER 120

Query: 121 EREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYSLIQEL 180
           EREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYSLIQEL
Sbjct: 121 EREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYSLIQEL 180

Query: 181 SPHWPSPSALFSAPLVSPISSPNLFNNLFDF 212
           SPHWPSPSALFSAPLVSPISSPNLFNNLFDF
Sbjct: 181 SPHWPSPSALFSAPLVSPISSPNLFNNLFDF 211

[Close]

BLAST of Carg24324 vs. NCBI nr
Match: XP_022932014.1 (protein MKS1-like [Cucurbita moschata])

HSP 1 Score: 394.8 bits (1013), Expect = 4.6e-106
Identity = 204/215 (94.88%), Postives = 207/215 (96.28%), Query Frame = 0


Query: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPP---- 60
           MNPPGYPAGGSPRKKEIQLQGPRPPQLRV+QDSRKIKKPPPHPQPIPPA RPPLPP    
Sbjct: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVSQDSRKIKKPPPHPQPIPPAERPPLPPGAGA 60

Query: 61  TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKASP 120
           TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGA+APDGDLSPAARLAAIEKASP
Sbjct: 61  TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGASAPDGDLSPAARLAAIEKASP 120

Query: 121 RPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYSL 180
           RPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATEL N  YSL
Sbjct: 121 RPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELPNFPYSL 180

Query: 181 IQELSPHWPSPSALFSAPLVSPISSPNLFNNLFDF 212
           IQELSPHWPSPSALFSAPLVSPISSPNLFN+LFDF
Sbjct: 181 IQELSPHWPSPSALFSAPLVSPISSPNLFNHLFDF 215

[Close]

BLAST of Carg24324 vs. NCBI nr
Match: XP_023512395.1 (protein MKS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 383.3 bits (983), Expect = 1.4e-102
Identity = 203/215 (94.42%), Postives = 204/215 (94.88%), Query Frame = 0


Query: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPP---- 60
           MNPPGYPAGGSPRKKEIQLQGPRPPQLRV+QDSRKIKKPPPHPQPIPPAGRPPLPP    
Sbjct: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVSQDSRKIKKPPPHPQPIPPAGRPPLPPGAGA 60

Query: 61  TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKASP 120
           TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKASP
Sbjct: 61  TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKASP 120

Query: 121 RPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYSL 180
           RP  EREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYSL
Sbjct: 121 RP--EREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYSL 180

Query: 181 IQELSPHWPSPSALFSAPLVSPISSPNLFNNLFDF 212
           IQELSPHWPSPSALF     SPISSPNLFNNLFDF
Sbjct: 181 IQELSPHWPSPSALF-----SPISSPNLFNNLFDF 208

[Close]

BLAST of Carg24324 vs. NCBI nr
Match: XP_022971712.1 (protein MKS1-like [Cucurbita maxima])

HSP 1 Score: 376.3 bits (965), Expect = 1.7e-100
Identity = 200/217 (92.17%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPP---- 60
           MNPPGYPAGGSPRKKEIQLQGPRPPQLRV+QDSRKIKKPPPHPQPIPP GRPPLPP    
Sbjct: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVSQDSRKIKKPPPHPQPIPPTGRPPLPPCAGA 60

Query: 61  TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKASP 120
           TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSS      GDLSPAARLAAIEKASP
Sbjct: 61  TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSS------GDLSPAARLAAIEKASP 120

Query: 121 RP--EREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSY 180
           RP  EREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSY
Sbjct: 121 RPEREREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSY 180

Query: 181 SLIQELSPHWPSPSALFSAPLVSPISSPNLFNNLFDF 212
           SLIQELSPHWPSPSALFSAPLVSPISSPNLF+ LF+F
Sbjct: 181 SLIQELSPHWPSPSALFSAPLVSPISSPNLFSTLFNF 211

[Close]

BLAST of Carg24324 vs. NCBI nr
Match: XP_038901805.1 (protein MKS1-like [Benincasa hispida])

HSP 1 Score: 340.9 bits (873), Expect = 7.9e-90
Identity = 184/217 (84.79%), Postives = 194/217 (89.40%), Query Frame = 0


Query: 1   MNPPGYPAGGS---PRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLP-- 60
           MNPPGY A GS   PRKKEIQLQGPRPPQLRVNQ+SRKIKKPPPHPQP+PP GRPPLP  
Sbjct: 1   MNPPGYSAAGSPTTPRKKEIQLQGPRPPQLRVNQESRKIKKPPPHPQPVPPGGRPPLPPG 60

Query: 61  PTQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGL-SSSGAAAPDGDLSPAARLAAIEKA 120
           P QWPQPLIIYDISPKVIHV ENNFMSVVQRLTGL SSS AAA DGDLSPAARLA IEKA
Sbjct: 61  PAQWPQPLIIYDISPKVIHVAENNFMSVVQRLTGLSSSSAAAATDGDLSPAARLATIEKA 120

Query: 121 SPRPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSY 180
           SPR   EREREI+VS+MMDL E+SVEL Q PGILSPAPA+LAPIPTG+FSPA E Q+L+Y
Sbjct: 121 SPR--SEREREINVSDMMDLAEVSVELGQIPGILSPAPATLAPIPTGYFSPAIEHQSLAY 180

Query: 181 SLIQELSPHWPSPSALFSAPLVSPISSPNLFNNLFDF 212
           SLI ELSPHWPSPSALFSAPLVSPISSPN+FNNLFDF
Sbjct: 181 SLIHELSPHWPSPSALFSAPLVSPISSPNIFNNLFDF 215

[Close]

BLAST of Carg24324 vs. ExPASy Swiss-Prot
Match: Q8LGD5 (Protein MKS1 OS=Arabidopsis thaliana OX=3702 GN=MKS1 PE=1 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 7.6e-27
Identity = 91/213 (42.72%), Postives = 123/213 (57.75%), Query Frame = 0


Query: 1   MNPPGYPAGGSP-----RKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLP 60
           M+P  Y AGG+P     +K+++Q+ GPRP  L V++DS KIKKPP HP P P   +P  P
Sbjct: 1   MDPSEYFAGGNPSDQQNQKRQLQICGPRPSPLSVHKDSHKIKKPPKHPAPPPNRDQP--P 60

Query: 61  PTQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSG--AAAPDGDLSPAARLAAIEK 120
           P    +P++IY +SPKV+H   + FM+VVQRLTG+SS     +   GD+SPAARLA+ E 
Sbjct: 61  PYIPREPVVIYAVSPKVVHATASEFMNVVQRLTGISSGVFLESGGGGDVSPAARLASTEN 120

Query: 121 ASPRPERE---REREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQ 180
           ASPR  +E   R+  ++++  M+  E +      PGILSP+PA L    TG FSP     
Sbjct: 121 ASPRGGKEPAARDETVEINTAME--EAAEFGGYAPGILSPSPALLPTASTGIFSPMYHQG 180

Query: 181 NLSYSLIQELSPHWPSPSALFS-APLVSPISSP 203
            +    I         P  LFS A  +SP  SP
Sbjct: 181 GMFSPAI---------PLGLFSPAGFMSPFRSP 200

[Close]

BLAST of Carg24324 vs. ExPASy Swiss-Prot
Match: F4HWF9 (Nuclear speckle RNA-binding protein B OS=Arabidopsis thaliana OX=3702 GN=NSRB PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.4e-09
Identity = 68/186 (36.56%), Postives = 84/186 (45.16%), Query Frame = 0


Query: 21  GPRPPQLRVNQDSRK-IKKP---PPHPQPIPP--------AGRPPLPPTQWPQPLIIYDI 80
           GP+P  L+V  DS K IKKP   PPHPQP PP          RPP      P P+ IY +
Sbjct: 13  GPKPTPLKVRGDSHKIIKKPPLAPPHPQPQPPQTHQQEPSQSRPP------PGPVNIYTV 72

Query: 81  SPKVIHVPENNFMSVVQRLTG---------LSSSGAAAP----------DGDLSPAARLA 140
           +P++IH   NNFM++VQRLTG          SSS  + P           G +SPAAR A
Sbjct: 73  TPRIIHTHPNNFMTLVQRLTGQTSTSTTSSSSSSSTSEPKDTSTMVDTSHGLISPAARFA 132

Query: 141 AIEKASPRPERER----EREID-------VSEMMDLGEISVELWQTPGILSPAPASLAPI 165
             EKA+   E       E  +D         +           +   GILSP P SL  +
Sbjct: 133 VTEKANISNELGTFVGGEGTMDQYYHHHHQEQPHQNRGFERPSFHHAGILSPGPYSLPSV 192

[Close]

BLAST of Carg24324 vs. ExPASy Swiss-Prot
Match: Q9LS54 (VQ motif-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=VQ20 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.6e-05
Identity = 31/72 (43.06%), Postives = 44/72 (61.11%), Query Frame = 0


Query: 22  PRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPPTQWPQPLIIYDISPKVIHVPENNFM 81
           P PP L+VN+DS  IKK PP P     A +P         P+IIY  +P++IH    +FM
Sbjct: 41  PSPPTLKVNKDSHVIKK-PPSPSSFSSAAKP-------RHPVIIYTHTPRIIHTNPKDFM 100

Query: 82  SVVQRLTGLSSS 94
           ++VQ+LTG++ S
Sbjct: 101 ALVQKLTGMTHS 104

[Close]

BLAST of Carg24324 vs. ExPASy TrEMBL
Match: A0A6J1EVV6 (protein MKS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438343 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.2e-106
Identity = 204/215 (94.88%), Postives = 207/215 (96.28%), Query Frame = 0


Query: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPP---- 60
           MNPPGYPAGGSPRKKEIQLQGPRPPQLRV+QDSRKIKKPPPHPQPIPPA RPPLPP    
Sbjct: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVSQDSRKIKKPPPHPQPIPPAERPPLPPGAGA 60

Query: 61  TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKASP 120
           TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGA+APDGDLSPAARLAAIEKASP
Sbjct: 61  TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGASAPDGDLSPAARLAAIEKASP 120

Query: 121 RPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYSL 180
           RPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATEL N  YSL
Sbjct: 121 RPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELPNFPYSL 180

Query: 181 IQELSPHWPSPSALFSAPLVSPISSPNLFNNLFDF 212
           IQELSPHWPSPSALFSAPLVSPISSPNLFN+LFDF
Sbjct: 181 IQELSPHWPSPSALFSAPLVSPISSPNLFNHLFDF 215

[Close]

BLAST of Carg24324 vs. ExPASy TrEMBL
Match: A0A6J1I2Q6 (protein MKS1-like OS=Cucurbita maxima OX=3661 GN=LOC111470379 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 8.2e-101
Identity = 200/217 (92.17%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPP---- 60
           MNPPGYPAGGSPRKKEIQLQGPRPPQLRV+QDSRKIKKPPPHPQPIPP GRPPLPP    
Sbjct: 1   MNPPGYPAGGSPRKKEIQLQGPRPPQLRVSQDSRKIKKPPPHPQPIPPTGRPPLPPCAGA 60

Query: 61  TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKASP 120
           TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSS      GDLSPAARLAAIEKASP
Sbjct: 61  TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSS------GDLSPAARLAAIEKASP 120

Query: 121 RP--EREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSY 180
           RP  EREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSY
Sbjct: 121 RPEREREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSY 180

Query: 181 SLIQELSPHWPSPSALFSAPLVSPISSPNLFNNLFDF 212
           SLIQELSPHWPSPSALFSAPLVSPISSPNLF+ LF+F
Sbjct: 181 SLIQELSPHWPSPSALFSAPLVSPISSPNLFSTLFNF 211

[Close]

BLAST of Carg24324 vs. ExPASy TrEMBL
Match: A0A5A7TBE4 (Protein MKS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00750 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 5.7e-86
Identity = 175/216 (81.02%), Postives = 188/216 (87.04%), Query Frame = 0


Query: 1   MNPPGYPAGGS---PRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLP-- 60
           MNPPGY A GS   PRKKEIQLQGPRPPQLRV+Q+SRKIKKPPPHPQP+PP GRPPLP  
Sbjct: 1   MNPPGYSAAGSPTTPRKKEIQLQGPRPPQLRVSQESRKIKKPPPHPQPVPPPGRPPLPPG 60

Query: 61  PTQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKAS 120
           P+QWPQPLIIYDISPKVIHV ENNFMSVVQRLTG SS+  A  DGDLSPAARLA IEKAS
Sbjct: 61  PSQWPQPLIIYDISPKVIHVAENNFMSVVQRLTGQSST--AVTDGDLSPAARLATIEKAS 120

Query: 121 PRPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYS 180
           PR   EREREI+VS+MMDL E+SVEL Q PGILSPAP +LAPIPTG+FSPA E Q+ SYS
Sbjct: 121 PR--SEREREINVSDMMDLAEVSVELGQIPGILSPAPGTLAPIPTGYFSPAIEPQSFSYS 180

Query: 181 LIQELSPHWPSPSALFSAPLVSPISSPNLFNNLFDF 212
           L  ELSPHWPSPSALFS PL+SPISSPN+FNNLFDF
Sbjct: 181 LFHELSPHWPSPSALFSTPLISPISSPNIFNNLFDF 212

[Close]

BLAST of Carg24324 vs. ExPASy TrEMBL
Match: A0A6J1G715 (protein MKS1-like OS=Cucurbita moschata OX=3662 GN=LOC111451450 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 5.7e-86
Identity = 177/216 (81.94%), Postives = 191/216 (88.43%), Query Frame = 0


Query: 1   MNPPGYPAGGS---PRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPP- 60
           MNPPG  AGGS   PRKKEIQLQGPRPPQLRVNQ+SRKIKKPPPHPQPIPP+GRPPLPP 
Sbjct: 1   MNPPGSSAGGSPNTPRKKEIQLQGPRPPQLRVNQESRKIKKPPPHPQPIPPSGRPPLPPA 60

Query: 61  -TQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKAS 120
            +QWPQPLIIYDISPKV+HV ENNFMSVVQRLTGLSS+ A++ DGDLSPAAR A IEKAS
Sbjct: 61  ASQWPQPLIIYDISPKVLHVAENNFMSVVQRLTGLSSA-ASSTDGDLSPAARFATIEKAS 120

Query: 121 PRPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYS 180
           PR   EREREIDVS+MMDL E+ VEL Q PGILSPAPASLAPI +GFFSPA E Q+ +YS
Sbjct: 121 PR--SEREREIDVSDMMDLTEVPVELGQNPGILSPAPASLAPISSGFFSPAIEPQSFTYS 180

Query: 181 LIQELSPHWPSPSALFSAPLVSPISSPNLFNNLFDF 212
           LI ELSPHWPSPSALF APLVSPISSPN+FN+LFDF
Sbjct: 181 LIHELSPHWPSPSALFPAPLVSPISSPNIFNHLFDF 213

[Close]

BLAST of Carg24324 vs. ExPASy TrEMBL
Match: A0A1S3CD17 (protein MKS1-like OS=Cucumis melo OX=3656 GN=LOC103499649 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 5.7e-86
Identity = 175/216 (81.02%), Postives = 188/216 (87.04%), Query Frame = 0


Query: 1   MNPPGYPAGGS---PRKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLP-- 60
           MNPPGY A GS   PRKKEIQLQGPRPPQLRV+Q+SRKIKKPPPHPQP+PP GRPPLP  
Sbjct: 1   MNPPGYSAAGSPTTPRKKEIQLQGPRPPQLRVSQESRKIKKPPPHPQPVPPPGRPPLPPG 60

Query: 61  PTQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSGAAAPDGDLSPAARLAAIEKAS 120
           P+QWPQPLIIYDISPKVIHV ENNFMSVVQRLTG SS+  A  DGDLSPAARLA IEKAS
Sbjct: 61  PSQWPQPLIIYDISPKVIHVAENNFMSVVQRLTGQSST--AVTDGDLSPAARLATIEKAS 120

Query: 121 PRPEREREREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQNLSYS 180
           PR   EREREI+VS+MMDL E+SVEL Q PGILSPAP +LAPIPTG+FSPA E Q+ SYS
Sbjct: 121 PR--SEREREINVSDMMDLAEVSVELGQIPGILSPAPGTLAPIPTGYFSPAIEPQSFSYS 180

Query: 181 LIQELSPHWPSPSALFSAPLVSPISSPNLFNNLFDF 212
           L  ELSPHWPSPSALFS PL+SPISSPN+FNNLFDF
Sbjct: 181 LFHELSPHWPSPSALFSTPLISPISSPNIFNNLFDF 212

[Close]

BLAST of Carg24324 vs. TAIR 10
Match: AT3G18690.1 (MAP kinase substrate 1 )

HSP 1 Score: 122.1 bits (305), Expect = 5.4e-28
Identity = 91/213 (42.72%), Postives = 123/213 (57.75%), Query Frame = 0


Query: 1   MNPPGYPAGGSP-----RKKEIQLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLP 60
           M+P  Y AGG+P     +K+++Q+ GPRP  L V++DS KIKKPP HP P P   +P  P
Sbjct: 1   MDPSEYFAGGNPSDQQNQKRQLQICGPRPSPLSVHKDSHKIKKPPKHPAPPPNRDQP--P 60

Query: 61  PTQWPQPLIIYDISPKVIHVPENNFMSVVQRLTGLSSSG--AAAPDGDLSPAARLAAIEK 120
           P    +P++IY +SPKV+H   + FM+VVQRLTG+SS     +   GD+SPAARLA+ E 
Sbjct: 61  PYIPREPVVIYAVSPKVVHATASEFMNVVQRLTGISSGVFLESGGGGDVSPAARLASTEN 120

Query: 121 ASPRPERE---REREIDVSEMMDLGEISVELWQTPGILSPAPASLAPIPTGFFSPATELQ 180
           ASPR  +E   R+  ++++  M+  E +      PGILSP+PA L    TG FSP     
Sbjct: 121 ASPRGGKEPAARDETVEINTAME--EAAEFGGYAPGILSPSPALLPTASTGIFSPMYHQG 180

Query: 181 NLSYSLIQELSPHWPSPSALFS-APLVSPISSP 203
            +    I         P  LFS A  +SP  SP
Sbjct: 181 GMFSPAI---------PLGLFSPAGFMSPFRSP 200

[Close]

BLAST of Carg24324 vs. TAIR 10
Match: AT1G21326.1 (VQ motif-containing protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.6e-14
Identity = 87/248 (35.08%), Postives = 108/248 (43.55%), Query Frame = 0


Query: 11  SPRKKEIQLQGPRPPQLRVNQDSRK-IKKP---PPHPQPIPP--------AGRPPLPPTQ 70
           SPR + I   GPRP  L+V  DS K IKKP   PPHPQP PP          RPP     
Sbjct: 5   SPRSRGI--LGPRPIPLKVRGDSHKIIKKPPLAPPHPQPQPPQTHQQEPSQSRPP----- 64

Query: 71  WPQPLIIYDISPKVIHVPENNFMSVVQRLTGLS---------SSGAAAP----------D 130
            P P+IIY +SP++IH   NNFM++VQRLTG +         SS  +AP           
Sbjct: 65  -PGPVIIYTVSPRIIHTHPNNFMTLVQRLTGKTSTSTTSSSYSSSTSAPKDASTMVDTSH 124

Query: 131 GDLSPAARLAAIEKASPRPE---------------REREREIDVSEMMDLGEISVELWQT 190
           G +SPAAR A  EKA+   E                         +           +  
Sbjct: 125 GLISPAARFAVTEKANISNELGTFVGGEGTMDQYYHYHHHHHHQEQQHQNQGFERPSFHH 184

Query: 191 PGILSPAPASLAPIPTGFFSP--ATELQNLSYSLIQELSPHWPSPSALFSAPLVSPISSP 211
            GILSP P SL  +   FFS    T+ Q  S S   + +    S  +   +P     SS 
Sbjct: 185 AGILSPGPNSLPSVSPDFFSTIGPTDPQGFS-SFFNDFNSILQSSPSKIQSP-----SSM 238

[Close]

BLAST of Carg24324 vs. TAIR 10
Match: AT1G21320.1 (nucleotide binding;nucleic acid binding )

HSP 1 Score: 64.7 bits (156), Expect = 1.0e-10
Identity = 68/186 (36.56%), Postives = 84/186 (45.16%), Query Frame = 0


Query: 21  GPRPPQLRVNQDSRK-IKKP---PPHPQPIPP--------AGRPPLPPTQWPQPLIIYDI 80
           GP+P  L+V  DS K IKKP   PPHPQP PP          RPP      P P+ IY +
Sbjct: 13  GPKPTPLKVRGDSHKIIKKPPLAPPHPQPQPPQTHQQEPSQSRPP------PGPVNIYTV 72

Query: 81  SPKVIHVPENNFMSVVQRLTG---------LSSSGAAAP----------DGDLSPAARLA 140
           +P++IH   NNFM++VQRLTG          SSS  + P           G +SPAAR A
Sbjct: 73  TPRIIHTHPNNFMTLVQRLTGQTSTSTTSSSSSSSTSEPKDTSTMVDTSHGLISPAARFA 132

Query: 141 AIEKASPRPERER----EREID-------VSEMMDLGEISVELWQTPGILSPAPASLAPI 165
             EKA+   E       E  +D         +           +   GILSP P SL  +
Sbjct: 133 VTEKANISNELGTFVGGEGTMDQYYHHHHQEQPHQNRGFERPSFHHAGILSPGPYSLPSV 192

[Close]

BLAST of Carg24324 vs. TAIR 10
Match: AT3G18360.1 (VQ motif-containing protein )

HSP 1 Score: 51.2 bits (121), Expect = 1.2e-06
Identity = 31/72 (43.06%), Postives = 44/72 (61.11%), Query Frame = 0


Query: 22  PRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPPTQWPQPLIIYDISPKVIHVPENNFM 81
           P PP L+VN+DS  IKK PP P     A +P         P+IIY  +P++IH    +FM
Sbjct: 41  PSPPTLKVNKDSHVIKK-PPSPSSFSSAAKP-------RHPVIIYTHTPRIIHTNPKDFM 100

Query: 82  SVVQRLTGLSSS 94
           ++VQ+LTG++ S
Sbjct: 101 ALVQKLTGMTHS 104

[Close]

BLAST of Carg24324 vs. TAIR 10
Match: AT1G68450.1 (VQ motif-containing protein )

HSP 1 Score: 42.0 bits (97), Expect = 7.1e-04
Identity = 30/73 (41.10%), Postives = 39/73 (53.42%), Query Frame = 0


Query: 18 QLQGPRPPQLRVNQDSRKIKKPPPHPQPIPPAGRPPLPPTQWPQPLIIYDISPKVIHVPE 77
          ++ G RP  L++  +S  IKK         P GR          P+IIY  SPKVIH   
Sbjct: 8  EINGSRPSSLKLAGESHTIKKTSSCKSKPRPHGR--------ASPVIIYAHSPKVIHTRA 67

Query: 78 NNFMSVVQRLTGL 91
           +FM++VQRLTGL
Sbjct: 68 EDFMALVQRLTGL 72

The following BLAST results are available for this feature:

BLAST of Carg24324 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571354.1	5.6e-112	100.00	Protein MKS1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011126.1 Prot...	[more]
XP_022932014.1	4.6e-106	94.88	protein MKS1-like [Cucurbita moschata]	[more]
XP_023512395.1	1.4e-102	94.42	protein MKS1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022971712.1	1.7e-100	92.17	protein MKS1-like [Cucurbita maxima]	[more]
XP_038901805.1	7.9e-90	84.79	protein MKS1-like [Benincasa hispida]	[more]

BLAST of Carg24324 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LGD5	7.6e-27	42.72	Protein MKS1 OS=Arabidopsis thaliana OX=3702 GN=MKS1 PE=1 SV=2	[more]
F4HWF9	1.4e-09	36.56	Nuclear speckle RNA-binding protein B OS=Arabidopsis thaliana OX=3702 GN=NSRB PE...	[more]
Q9LS54	1.6e-05	43.06	VQ motif-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=VQ20 PE=2 SV=1	[more]

BLAST of Carg24324 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EVV6	2.2e-106	94.88	protein MKS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438343 PE=4 SV=1	[more]
A0A6J1I2Q6	8.2e-101	92.17	protein MKS1-like OS=Cucurbita maxima OX=3661 GN=LOC111470379 PE=4 SV=1	[more]
A0A5A7TBE4	5.7e-86	81.02	Protein MKS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00...	[more]
A0A6J1G715	5.7e-86	81.94	protein MKS1-like OS=Cucurbita moschata OX=3662 GN=LOC111451450 PE=4 SV=1	[more]
A0A1S3CD17	5.7e-86	81.02	protein MKS1-like OS=Cucumis melo OX=3656 GN=LOC103499649 PE=4 SV=1	[more]

BLAST of Carg24324 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18690.1	5.4e-28	42.72	MAP kinase substrate 1	[more]
AT1G21326.1	2.6e-14	35.08	VQ motif-containing protein	[more]
AT1G21320.1	1.0e-10	36.56	nucleotide binding;nucleic acid binding	[more]
AT3G18360.1	1.2e-06	43.06	VQ motif-containing protein	[more]
AT1G68450.1	7.1e-04	41.10	VQ motif-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008889	VQ	PFAM	PF05678	VQ	coord: 68..92 e-value: 8.5E-10 score: 37.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..58
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..58
None	No IPR available	PANTHER	PTHR33143:SF50	PROTEIN MKS1	coord: 11..209
IPR039607	VQ motif-containing protein 8/17/18/20/21/25	PANTHER	PTHR33143	F16F4.1 PROTEIN-RELATED	coord: 11..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24324-RA	Carg24324-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005634	nucleus