Carg24279 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24279
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	50S ribosomal protein L27
Location	Carg_Chr15: 485952 .. 486951 (+)
RNA-Seq Expression	Carg24279
Synteny	Carg24279

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTAGCCATTGTGGTGGTACGGAACCACTAAATGAAGGCGGTTTAAATTTCATAATTGGGGGTGAATACAAGTGCATACAAGCGAACCCTGCCGGTCCAAGATTCGCATTCGGTGGCTTGGGGCGGTTCTGGGTCGATTTCTTGTATTCGCTTCACAGTTCCAATCTTCGGGAGCCGAGGAAATCCCTGTTGCAGCCATGAGTTTAAACCTAATTGGCGCATTCAGAGGCCTCTCTCTGGCCTCTTCTTCTTCGTCCTCTTTCTTCAAAGGCGATTTCGGATCTATTCAACTGCCTCCCAAGCTCTCCGTCTCGTTCCCTAACAGATTCCCTTTAACCATTGAAAATGCACACAAGAAGGGAGCTGGAAGCACGAAGAACGGCCGAGATTCTAAGGGCCAGAGGCTCGGCGTTAAAATCTACGGCGATCAGGTTGCCAAGCCTGGTTCTATCATCGTTCGTCAGCGTGGAACCAAGGTAGTTTCTCAGTCCCAAATCATTTTCCAATTCGATTTTGAAAATTGTATGTTGAGGAAGTTGGTGCTCGTTCCGCACATTGTTTAGGGCATTCCTAATTTACGAACATACATAGATTGTTTTGTAATTGAGTTCCATGGTGTCTGTTTTTGTACAAATGATTTCAGTTCCATGCTGGAAAGAATGTGGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGACGGGCTTGTAAAATTCGAGAAGTATGGACCTGACAAGAAGAAGGTTTCACTTCGAACTGGACCTTTCAAGATAGCTTGCAAGAATGAAACAAAACTGAGATGTATGTATTTGATTGCAGGTGAGTGTGTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTACAGAGCAAGGAAGAGGGAGTATTTCAGGCTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCACTATTGCTCAACCCCAATTAGTATTAGCTTCCACTGACGCTACAGAAACCAATCCGGTTTGCTGA

mRNA sequence

ATGCTAGCCATTGTGGTGTTCCAATCTTCGGGAGCCGAGGAAATCCCTGTTGCAGCCATGAGTTTAAACCTAATTGGCGCATTCAGAGGCCTCTCTCTGGCCTCTTCTTCTTCGTCCTCTTTCTTCAAAGGCGATTTCGGATCTATTCAACTGCCTCCCAAGCTCTCCGTCTCGTTCCCTAACAGATTCCCTTTAACCATTGAAAATGCACACAAGAAGGGAGCTGGAAGCACGAAGAACGGCCGAGATTCTAAGGGCCAGAGGCTCGGCGTTAAAATCTACGGCGATCAGGTTGCCAAGCCTGGTTCTATCATCGTTCGTCAGCGTGGAACCAAGTTCCATGCTGGAAAGAATGTGGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGACGGGCTTGTAAAATTCGAGAAGTATGGACCTGACAAGAAGAAGGTGAGTGTGTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTACAGAGCAAGGAAGAGGGAGTATTTCAGGCTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCACTATTGCTCAACCCCAATTAGTATTAGCTTCCACTGACGCTACAGAAACCAATCCGGTTTGCTGA

Coding sequence (CDS)

ATGCTAGCCATTGTGGTGTTCCAATCTTCGGGAGCCGAGGAAATCCCTGTTGCAGCCATGAGTTTAAACCTAATTGGCGCATTCAGAGGCCTCTCTCTGGCCTCTTCTTCTTCGTCCTCTTTCTTCAAAGGCGATTTCGGATCTATTCAACTGCCTCCCAAGCTCTCCGTCTCGTTCCCTAACAGATTCCCTTTAACCATTGAAAATGCACACAAGAAGGGAGCTGGAAGCACGAAGAACGGCCGAGATTCTAAGGGCCAGAGGCTCGGCGTTAAAATCTACGGCGATCAGGTTGCCAAGCCTGGTTCTATCATCGTTCGTCAGCGTGGAACCAAGTTCCATGCTGGAAAGAATGTGGGGCTTGGCAAGGACCATACAATTTTCTCATTGATTGACGGGCTTGTAAAATTCGAGAAGTATGGACCTGACAAGAAGAAGGTGAGTGTGTACCCAAGAGAAGTGCAGCCTGAGAATCCAAACAGCTACAGAGCAAGGAAGAGGGAGTATTTCAGGCTGCAAAGAGAGCGCAAGAAAGCAAGAAAGGAAGGCACTATTGCTCAACCCCAATTAGTATTAGCTTCCACTGACGCTACAGAAACCAATCCGGTTTGCTGA

Protein sequence

MLAIVVFQSSGAEEIPVAAMSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATETNPVC

Homology

[Close]

BLAST of Carg24279 vs. NCBI nr
Match: KAG7015950.1 (50S ribosomal protein L27, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 394.4 bits (1012), Expect = 5.8e-106
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 1   MLAIVVFQSSGAEEIPVAAMSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFP 60
           MLAIVVFQSSGAEEIPVAAMSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFP
Sbjct: 1   MLAIVVFQSSGAEEIPVAAMSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFP 60

Query: 61  NRFPLTIENAHKKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVG 120
           NRFPLTIENAHKKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVG
Sbjct: 61  NRFPLTIENAHKKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVG 120

Query: 121 LGKDHTIFSLIDGLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKAR 180
           LGKDHTIFSLIDGLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKAR
Sbjct: 121 LGKDHTIFSLIDGLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKAR 180

Query: 181 KEGTIAQPQLVLASTDATETNPVC 205
           KEGTIAQPQLVLASTDATETNPVC
Sbjct: 181 KEGTIAQPQLVLASTDATETNPVC 204

[Close]

BLAST of Carg24279 vs. NCBI nr
Match: XP_023550670.1 (50S ribosomal protein L27, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023550671.1 50S ribosomal protein L27, chloroplastic [Cucurbita pepo subsp. pepo] >KAG6578368.1 50S ribosomal protein L27, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 362.5 bits (929), Expect = 2.5e-96
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 20  MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 79
           MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK
Sbjct: 1   MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 60

Query: 80  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 139
           NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 61  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120

Query: 140 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 199
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE
Sbjct: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 180

Query: 200 TNPVC 205
           TNPVC
Sbjct: 181 TNPVC 185

[Close]

BLAST of Carg24279 vs. NCBI nr
Match: XP_022993762.1 (50S ribosomal protein L27, chloroplastic [Cucurbita maxima] >XP_022993763.1 50S ribosomal protein L27, chloroplastic [Cucurbita maxima])

HSP 1 Score: 359.8 bits (922), Expect = 1.6e-95
Identity = 183/185 (98.92%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 20  MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 79
           MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK
Sbjct: 1   MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 60

Query: 80  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 139
           NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 61  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120

Query: 140 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 199
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG+I+QPQLVLASTDATE
Sbjct: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGSISQPQLVLASTDATE 180

Query: 200 TNPVC 205
           TNPVC
Sbjct: 181 TNPVC 185

[Close]

BLAST of Carg24279 vs. NCBI nr
Match: XP_022939083.1 (50S ribosomal protein L27, chloroplastic [Cucurbita moschata] >XP_022939084.1 50S ribosomal protein L27, chloroplastic [Cucurbita moschata] >XP_022939085.1 50S ribosomal protein L27, chloroplastic [Cucurbita moschata])

HSP 1 Score: 358.2 bits (918), Expect = 4.6e-95
Identity = 183/185 (98.92%), Postives = 183/185 (98.92%), Query Frame = 0


Query: 20  MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 79
           MSLNLIGAFRGLSLA SSSSSFFKGDFGSIQLPPKLSVSFP RFPLTIENAHKKGAGSTK
Sbjct: 1   MSLNLIGAFRGLSLAPSSSSSFFKGDFGSIQLPPKLSVSFPTRFPLTIENAHKKGAGSTK 60

Query: 80  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 139
           NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 61  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120

Query: 140 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 199
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE
Sbjct: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 180

Query: 200 TNPVC 205
           TNPVC
Sbjct: 181 TNPVC 185

[Close]

BLAST of Carg24279 vs. NCBI nr
Match: XP_022133707.1 (50S ribosomal protein L27, chloroplastic [Momordica charantia])

HSP 1 Score: 352.4 bits (903), Expect = 2.5e-93
Identity = 177/190 (93.16%), Postives = 186/190 (97.89%), Query Frame = 0


Query: 15  IPVAAMSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKG 74
           +PVAAMSLNL+GAFRGLSL SSSSSSFF+GDF S+Q PPK S+SFPNRFPLTIENAHKKG
Sbjct: 1   MPVAAMSLNLVGAFRGLSLVSSSSSSFFRGDFASVQPPPKFSLSFPNRFPLTIENAHKKG 60

Query: 75  AGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL 134
           AGSTKNGRDSKGQRLGVKI+GDQ+AKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL
Sbjct: 61  AGSTKNGRDSKGQRLGVKIFGDQLAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL 120

Query: 135 VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLAS 194
           VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG+IAQPQLVLAS
Sbjct: 121 VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGSIAQPQLVLAS 180

Query: 195 TDATETNPVC 205
           +DATETNPVC
Sbjct: 181 SDATETNPVC 190

[Close]

BLAST of Carg24279 vs. ExPASy Swiss-Prot
Match: P82190 (50S ribosomal protein L27, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL27 PE=1 SV=2)

HSP 1 Score: 283.1 bits (723), Expect = 2.5e-75
Identity = 148/190 (77.89%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 19  AMSLNLIGAFRGLSLASSSSSSFFKGDFG--SIQLPPKLSVSFPNRFPLTIENAHKKGAG 78
           +MS NL+ +FRG+SL+SSSSSSFFKG+FG  S++LP K  +S  + FPLTIE+AHKKGAG
Sbjct: 6   SMSFNLMASFRGMSLSSSSSSSFFKGEFGPSSLRLPNKSPLSV-SPFPLTIESAHKKGAG 65

Query: 79  STKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVK 138
           STKNGRDSKGQRLGVKIYGDQVAKPG+II+RQRGTKFH GKNVG+GKDHTIF+LIDGLVK
Sbjct: 66  STKNGRDSKGQRLGVKIYGDQVAKPGAIIIRQRGTKFHPGKNVGIGKDHTIFALIDGLVK 125

Query: 139 FEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLAS-- 198
           FEKYGPDKKKVSVYPRE+QPENPNSYRARKRE FRLQRE+KKAR+EG   QPQL+LAS  
Sbjct: 126 FEKYGPDKKKVSVYPREIQPENPNSYRARKRENFRLQREKKKARREGYSFQPQLILASAA 185

Query: 199 TDATETNPVC 205
           TD  + + VC
Sbjct: 186 TDNADESAVC 194

[Close]

BLAST of Carg24279 vs. ExPASy Swiss-Prot
Match: P30155 (50S ribosomal protein L27, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL27 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 8.3e-71
Identity = 139/170 (81.76%), Postives = 153/170 (90.00%), Query Frame = 0


Query: 19  AMSLNLIGAFRGLSLASSSSSSFFKGDFG-SIQLPPKLSVSFPNRFPLTIENAHKKGAGS 78
           A+S +L+GAF+GLSLA  SSSSF KGDFG +  + PK SVSFP + PLTIE+AHKKGAGS
Sbjct: 2   AVSFSLVGAFKGLSLA--SSSSFLKGDFGAAFPVAPKFSVSFPLKSPLTIESAHKKGAGS 61

Query: 79  TKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKF 138
           TKNGRDS GQRLGVKI+GDQVAKPGSIIVRQRGTKFH GKNVGLGKDHTIFSLIDGLVKF
Sbjct: 62  TKNGRDSPGQRLGVKIFGDQVAKPGSIIVRQRGTKFHPGKNVGLGKDHTIFSLIDGLVKF 121

Query: 139 EKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQ 188
           EK+GPD+KK+SVYPREVQPENPNSYR RKRE FRLQRER+KAR+EG I Q
Sbjct: 122 EKFGPDRKKISVYPREVQPENPNSYRNRKRESFRLQRERRKARREGVILQ 169

[Close]

BLAST of Carg24279 vs. ExPASy Swiss-Prot
Match: Q9FLN4 (50S ribosomal protein L27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL27 PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 6.6e-68
Identity = 144/198 (72.73%), Postives = 161/198 (81.31%), Query Frame = 0


Query: 19  AMSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLS--VSFPNR-----FPLTIENAH 78
           +MSLNLIGAF+GLSL  SS+SSF +GD   +   PK S  V+ P        PLTIE+AH
Sbjct: 6   SMSLNLIGAFKGLSL--SSTSSFLRGD---LSFSPKTSFTVTLPLENLQAPIPLTIESAH 65

Query: 79  KKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLI 138
           KKGAGSTKNGRDS GQRLGVKIYGDQVAKPG+IIVRQRGTKFHAGKNVG+GKDHTIFSLI
Sbjct: 66  KKGAGSTKNGRDSPGQRLGVKIYGDQVAKPGAIIVRQRGTKFHAGKNVGIGKDHTIFSLI 125

Query: 139 DGLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG---TIAQP 198
           DGLVKFEK+GPD+KK+SVYPRE+ PENPNSYRARKRE FRLQRE+KKAR+E    T+  P
Sbjct: 126 DGLVKFEKFGPDRKKISVYPREIVPENPNSYRARKRENFRLQREKKKARRENYSYTLPTP 185

Query: 199 QLVL--ASTDATETNPVC 205
           +LVL  AS D  E NP C
Sbjct: 186 ELVLASASVDDAEANPEC 198

[Close]

BLAST of Carg24279 vs. ExPASy Swiss-Prot
Match: O65037 (50S ribosomal protein L27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RPL27 PE=2 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 6.3e-63
Identity = 132/196 (67.35%), Postives = 156/196 (79.59%), Query Frame = 0


Query: 17  VAAMSLNLIGAFRGLSLA----SSSSSSFFKGDFGSIQLPPKLSVSFPNRFP---LTIEN 76
           +A+M+  L+GAF+G+SL+    SSSS+SF + D  S+ +   + +  P   P   LTI+ 
Sbjct: 1   MASMAFTLVGAFKGMSLSSPCHSSSSASFLRADRVSLSVGGGVGMGVPMTMPVRRLTIQM 60

Query: 77  AHKKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 136
           AHKKGAGSTKNGRDS GQRLGVKIYGDQVAKPG+II+RQRGT+ + G NVG+GKDHT+FS
Sbjct: 61  AHKKGAGSTKNGRDSPGQRLGVKIYGDQVAKPGAIIIRQRGTRVYPGNNVGMGKDHTLFS 120

Query: 137 LIDGLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQ 196
           LIDGLVKFEKYGPDKKKVSVYP E QPENPNSYRARKREYFR+QRERKKAR EG I + Q
Sbjct: 121 LIDGLVKFEKYGPDKKKVSVYPYEKQPENPNSYRARKREYFRMQRERKKARAEG-IVEVQ 180

Query: 197 LVLASTD-ATETNPVC 205
           LVLA+ D + E N  C
Sbjct: 181 LVLAAADESPEVNADC 195

[Close]

BLAST of Carg24279 vs. ExPASy Swiss-Prot
Match: A6Q4D3 (50S ribosomal protein L27 OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=rpmA PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.3e-28
Identity = 59/84 (70.24%), Postives = 71/84 (84.52%), Query Frame = 0


Query: 70  AHKKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 129
           AHKKG GST+N RDS G+RLGVK +G +  + G+II+RQRGTK H G NVGLGKDHTIF+
Sbjct: 2   AHKKGQGSTQNNRDSAGRRLGVKKFGGEFVRAGNIIIRQRGTKVHPGNNVGLGKDHTIFA 61

Query: 130 LIDGLVKFEKYGPDKKKVSVYPRE 154
           LIDG+VKFE+YG D++KVSVYP E
Sbjct: 62  LIDGVVKFERYGKDRQKVSVYPVE 85

[Close]

BLAST of Carg24279 vs. ExPASy TrEMBL
Match: A0A6J1K388 (50S ribosomal protein L27, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111489672 PE=3 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 7.7e-96
Identity = 183/185 (98.92%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 20  MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 79
           MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK
Sbjct: 1   MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 60

Query: 80  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 139
           NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 61  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120

Query: 140 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 199
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG+I+QPQLVLASTDATE
Sbjct: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGSISQPQLVLASTDATE 180

Query: 200 TNPVC 205
           TNPVC
Sbjct: 181 TNPVC 185

[Close]

BLAST of Carg24279 vs. ExPASy TrEMBL
Match: A0A6J1FG23 (50S ribosomal protein L27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445091 PE=3 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.2e-95
Identity = 183/185 (98.92%), Postives = 183/185 (98.92%), Query Frame = 0


Query: 20  MSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKGAGSTK 79
           MSLNLIGAFRGLSLA SSSSSFFKGDFGSIQLPPKLSVSFP RFPLTIENAHKKGAGSTK
Sbjct: 1   MSLNLIGAFRGLSLAPSSSSSFFKGDFGSIQLPPKLSVSFPTRFPLTIENAHKKGAGSTK 60

Query: 80  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 139
           NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK
Sbjct: 61  NGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGLVKFEK 120

Query: 140 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 199
           YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE
Sbjct: 121 YGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLASTDATE 180

Query: 200 TNPVC 205
           TNPVC
Sbjct: 181 TNPVC 185

[Close]

BLAST of Carg24279 vs. ExPASy TrEMBL
Match: A0A6J1BXH1 (50S ribosomal protein L27, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006224 PE=3 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.2e-93
Identity = 177/190 (93.16%), Postives = 186/190 (97.89%), Query Frame = 0


Query: 15  IPVAAMSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKG 74
           +PVAAMSLNL+GAFRGLSL SSSSSSFF+GDF S+Q PPK S+SFPNRFPLTIENAHKKG
Sbjct: 1   MPVAAMSLNLVGAFRGLSLVSSSSSSFFRGDFASVQPPPKFSLSFPNRFPLTIENAHKKG 60

Query: 75  AGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL 134
           AGSTKNGRDSKGQRLGVKI+GDQ+AKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL
Sbjct: 61  AGSTKNGRDSKGQRLGVKIFGDQLAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL 120

Query: 135 VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLAS 194
           VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG+IAQPQLVLAS
Sbjct: 121 VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGSIAQPQLVLAS 180

Query: 195 TDATETNPVC 205
           +DATETNPVC
Sbjct: 181 SDATETNPVC 190

[Close]

BLAST of Carg24279 vs. ExPASy TrEMBL
Match: A0A6J1JNS6 (50S ribosomal protein L27, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111486989 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.8e-92
Identity = 175/190 (92.11%), Postives = 182/190 (95.79%), Query Frame = 0


Query: 15  IPVAAMSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKG 74
           +PV AMSLNL+GAFRGLSL SSSSSSFFKGDFGSIQ PP LSVSFPNRFPLTIE+AHKKG
Sbjct: 1   MPVTAMSLNLVGAFRGLSLVSSSSSSFFKGDFGSIQPPPNLSVSFPNRFPLTIESAHKKG 60

Query: 75  AGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL 134
           AGSTKNGRDSKGQRLGVKI+GDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL
Sbjct: 61  AGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL 120

Query: 135 VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLAS 194
           VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG +AQ QL+LAS
Sbjct: 121 VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGVVAQAQLILAS 180

Query: 195 TDATETNPVC 205
           TDA  TNP+C
Sbjct: 181 TDAPVTNPIC 190

[Close]

BLAST of Carg24279 vs. ExPASy TrEMBL
Match: A0A6J1E4G2 (50S ribosomal protein L27, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111430701 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.0e-91
Identity = 175/189 (92.59%), Postives = 182/189 (96.30%), Query Frame = 0


Query: 15  IPVAAMSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLSVSFPNRFPLTIENAHKKG 74
           +PV AM+LNL+GAFRGLSL SSSSSSFFKGDFGSIQ PP LSVSFPNRFPLTIE+AHKKG
Sbjct: 1   MPVTAMTLNLVGAFRGLSLVSSSSSSFFKGDFGSIQPPPNLSVSFPNRFPLTIESAHKKG 60

Query: 75  AGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL 134
           AGSTKNGRDSKGQRLGVKI+GDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL
Sbjct: 61  AGSTKNGRDSKGQRLGVKIFGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLIDGL 120

Query: 135 VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGTIAQPQLVLAS 194
           VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG +AQ QLVLAS
Sbjct: 121 VKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEGVVAQAQLVLAS 180

Query: 195 TDATETNPV 204
           TDA ETNP+
Sbjct: 181 TDAPETNPI 189

[Close]

BLAST of Carg24279 vs. TAIR 10
Match: AT5G40950.1 (ribosomal protein large subunit 27 )

HSP 1 Score: 258.5 bits (659), Expect = 4.7e-69
Identity = 144/198 (72.73%), Postives = 161/198 (81.31%), Query Frame = 0


Query: 19  AMSLNLIGAFRGLSLASSSSSSFFKGDFGSIQLPPKLS--VSFPNR-----FPLTIENAH 78
           +MSLNLIGAF+GLSL  SS+SSF +GD   +   PK S  V+ P        PLTIE+AH
Sbjct: 6   SMSLNLIGAFKGLSL--SSTSSFLRGD---LSFSPKTSFTVTLPLENLQAPIPLTIESAH 65

Query: 79  KKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFSLI 138
           KKGAGSTKNGRDS GQRLGVKIYGDQVAKPG+IIVRQRGTKFHAGKNVG+GKDHTIFSLI
Sbjct: 66  KKGAGSTKNGRDSPGQRLGVKIYGDQVAKPGAIIVRQRGTKFHAGKNVGIGKDHTIFSLI 125

Query: 139 DGLVKFEKYGPDKKKVSVYPREVQPENPNSYRARKREYFRLQRERKKARKEG---TIAQP 198
           DGLVKFEK+GPD+KK+SVYPRE+ PENPNSYRARKRE FRLQRE+KKAR+E    T+  P
Sbjct: 126 DGLVKFEKFGPDRKKISVYPREIVPENPNSYRARKRENFRLQREKKKARRENYSYTLPTP 185

Query: 199 QLVL--ASTDATETNPVC 205
           +LVL  AS D  E NP C
Sbjct: 186 ELVLASASVDDAEANPEC 198

[Close]

BLAST of Carg24279 vs. TAIR 10
Match: AT2G16930.2 (Ribosomal protein L27 family protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.4e-19
Identity = 45/70 (64.29%), Postives = 55/70 (78.57%), Query Frame = 0


Query: 70  AHKKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 129
           A KK AGSTKNGRDS  + LGVK +G +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 130 LIDGLVKFEK 140
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

[Close]

BLAST of Carg24279 vs. TAIR 10
Match: AT2G16930.1 (Ribosomal protein L27 family protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.4e-19
Identity = 45/70 (64.29%), Postives = 55/70 (78.57%), Query Frame = 0


Query: 70  AHKKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 129
           A KK AGSTKNGRDS  + LGVK +G +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 130 LIDGLVKFEK 140
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

[Close]

BLAST of Carg24279 vs. TAIR 10
Match: AT2G16930.3 (Ribosomal protein L27 family protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.4e-19
Identity = 45/70 (64.29%), Postives = 55/70 (78.57%), Query Frame = 0


Query: 70  AHKKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 129
           A KK AGSTKNGRDS  + LGVK +G +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 130 LIDGLVKFEK 140
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

[Close]

BLAST of Carg24279 vs. TAIR 10
Match: AT5G15220.1 (Ribosomal protein L27 family protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.4e-19
Identity = 45/70 (64.29%), Postives = 55/70 (78.57%), Query Frame = 0


Query: 70  AHKKGAGSTKNGRDSKGQRLGVKIYGDQVAKPGSIIVRQRGTKFHAGKNVGLGKDHTIFS 129
           A KK AGSTKNGRDS  + LGVK +G +   PG+IIVRQRGT+FH G  VG+GKDHT+F+
Sbjct: 47  ATKKTAGSTKNGRDSNPKFLGVKKFGGESVIPGNIIVRQRGTRFHPGDYVGIGKDHTLFA 106

Query: 130 LIDGLVKFEK 140
           L +G V+FEK
Sbjct: 107 LKEGRVRFEK 116

The following BLAST results are available for this feature:

BLAST of Carg24279 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015950.1	5.8e-106	100.00	50S ribosomal protein L27, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
XP_023550670.1	2.5e-96	100.00	50S ribosomal protein L27, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023550...	[more]
XP_022993762.1	1.6e-95	98.92	50S ribosomal protein L27, chloroplastic [Cucurbita maxima] >XP_022993763.1 50S ...	[more]
XP_022939083.1	4.6e-95	98.92	50S ribosomal protein L27, chloroplastic [Cucurbita moschata] >XP_022939084.1 50...	[more]
XP_022133707.1	2.5e-93	93.16	50S ribosomal protein L27, chloroplastic [Momordica charantia]	[more]

BLAST of Carg24279 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P82190	2.5e-75	77.89	50S ribosomal protein L27, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL27 P...	[more]
P30155	8.3e-71	81.76	50S ribosomal protein L27, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL27 P...	[more]
Q9FLN4	6.6e-68	72.73	50S ribosomal protein L27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL2...	[more]
O65037	6.3e-63	67.35	50S ribosomal protein L27, chloroplastic OS=Oryza sativa subsp. japonica OX=3994...	[more]
A6Q4D3	1.3e-28	70.24	50S ribosomal protein L27 OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=rpm...	[more]

BLAST of Carg24279 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K388	7.7e-96	98.92	50S ribosomal protein L27, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A6J1FG23	2.2e-95	98.92	50S ribosomal protein L27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1BXH1	1.2e-93	93.16	50S ribosomal protein L27, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1JNS6	6.8e-92	92.11	50S ribosomal protein L27, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1E4G2	2.0e-91	92.59	50S ribosomal protein L27, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of Carg24279 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G40950.1	4.7e-69	72.73	ribosomal protein large subunit 27	[more]
AT2G16930.2	4.4e-19	64.29	Ribosomal protein L27 family protein	[more]
AT2G16930.1	4.4e-19	64.29	Ribosomal protein L27 family protein	[more]
AT2G16930.3	4.4e-19	64.29	Ribosomal protein L27 family protein	[more]
AT5G15220.1	4.4e-19	64.29	Ribosomal protein L27 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 162..182
None	No IPR available	GENE3D	2.40.50.100		coord: 74..182 e-value: 4.6E-29 score: 102.7
None	No IPR available	PANTHER	PTHR15893:SF0	39S RIBOSOMAL PROTEIN L27, MITOCHONDRIAL	coord: 41..197
None	No IPR available	SUPERFAMILY	110324	Ribosomal L27 protein-like	coord: 70..151
IPR001684	Ribosomal protein L27	PRINTS	PR00063	RIBOSOMALL27	coord: 97..121 score: 62.92 coord: 122..146 score: 49.23 coord: 72..96 score: 66.77
IPR001684	Ribosomal protein L27	PFAM	PF01016	Ribosomal_L27	coord: 70..149 e-value: 8.2E-36 score: 121.9
IPR001684	Ribosomal protein L27	TIGRFAM	TIGR00062	TIGR00062	coord: 69..149 e-value: 1.3E-36 score: 122.5
IPR001684	Ribosomal protein L27	PANTHER	PTHR15893	RIBOSOMAL PROTEIN L27	coord: 41..197
IPR001684	Ribosomal protein L27	HAMAP	MF_00539	Ribosomal_L27	coord: 69..152 score: 27.131874
IPR018261	Ribosomal protein L27, conserved site	PROSITE	PS00831	RIBOSOMAL_L27	coord: 102..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24279-RA	Carg24279-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005840	ribosome
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0003735	structural constituent of ribosome