Carg24192 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24192
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	transcription factor bHLH35-like
Location	Carg_Chr12: 8484802 .. 8488107 (+)
RNA-Seq Expression	Carg24192
Synteny	Carg24192

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAACATTCTCGACGAGTATCTGAAATATTGGGAAACGGACATGTTTCTGCTGACGGAAGACTCCGGCAGGTTTCTTTTCGGAATCCGTTGTTTTTTTAATTTTTTTTAATTTTTTTTTTAATATCCTTTGAAATTAGCGGCGGTTGGGGACTGGAGGAAGCATTTTACGGTGCTTACAATTCGAGCTCGCCGGACGGGAAGGTGTCGCCGTCGTCGCCGTCGGAGGCGTCGAAGAATATCGTATCGGAGAGAAACCGGAGAAAGAAGCTTAACGATAGGCTTATTGCTCTCCGAGCTGTGGTCCCCAATATCACCAAAGTGAGATTTTCATTTTTTTTTAATTAAATTTATAAACTTTTTTTTTAAAAAAAAAAATTACAAATATTATTTCGCTTATAATTTTTCATGTTTTATTATTTATTTTTGTTTATATATTTTTAAAAACAAACCTAAATTTTGAAATTTTACAAAATTGGTTTTTATTTTTAGAATTTGACTACAAATTTTAATATTTTCATAAATATTTTTGAATAAGTGACAATATCATAAATATTTTTGAAAAATTAAATAAAATTATATCAGTTAAATTTATAAAAATAATAAAACATTAGAGACTAAATATAAATTAGCATCATTATTATTAAAAAAAAATTTAGTTAAATTAGCCGTTCAGGGGCAATTTAGTCATTACACATAATAACTTTTTTAAGTTATTTAATTAATAAAAATACAGTACTTTCCATTTTTCAAAATTCCAATAATACCCTTAAAAAAAACTAAAAATATTCTAAAATTTTCATCAAAGTTAAAAAATATCCTTATTTTAAAAATTTTTTATTAATATTTTTAAAACTCACTATAAAAAATAATATAAATATTTTTAATTTTACTAATTGTTAATAAAAATATTTTTAAAATAATATATTTTTTTTACAGGATATTAATATTTATAATTTTTTTTTTTTTTTTTAATATTTAAAGTTTGTGTTGGTGATGACAGATGGACAAGGCGTCAATAATAAAAGATGCCATCGTATACATCCAAGAGTTGCGAGCGGAAGAGAAGAGAATCGAAATAGAGATTTCCGATCTGGAATCAAGCGTAAACAACAACGGATCAAGAACCACAAATCAATGGAACGACGAAAACGGAAATCGAATTTGGACGAACATGAAATGGAATCGACAAACAACTGACGCTTCATCGTCTTCCTCGATCGAAGTTCTCGATGTAAGTGAGTTTGAATCTCAAAATTTGAATTTCTAGGACTTTTTGTTTGTTTGATTGAAACTTGTTGTTGTTGTTGTTAGTTGAGTGTGAATTACGTGGGCGAGAAGGTGATGGTGGTGAGTTTGACGTGCTTACGCCGAGGCAACACGATATTGAAGGTTTGGGAAGTGATTGAATCGTTGAAGTTGAAGATCATTACTGCTAATATCAGTGTCTTCGCAAACAAGCTCTTGAACACGCTCTTCATTGAGGTTAGTGTCAAATTGAAAGTTTAGGTTATGTTAGACGTTGTACCACTCGAGTAAGTGAGGGGTACATGGATTAACTACAACACAAAATGTGTATTCTCGATCAATAGGTCAAAAGTTTGAATTTCACGTCCCGAAAATTATTCTTTTTTCTCTCTCCTCAATCCTGCCCCTTTTCTCTCTTTTATATGTCGTTTTCCATGTCGGTTTCATTTCAACTACTTTTTTTTTTTTTCTTCCATGATTTAGCTTTGTTCTGTCAGCTCGTGTTATTGTCTCCTACCTCCATATTGTCGTATCAATCTGAGTCGCCTAGCTCTAGCTCTCAATTAATTTAGGTATTATATAGGATTCTAATCCAATGGGATAAGCCAAACATTTCATAAACGATGTTGAAAACAACAGTACTCTTGTAATTAGGAATCACGGATCTCCACACAATAGTATGATATTATTCATTTTTAGCATAAGCTCTCGTGTCTTTACTTTTTGTTTCTCCCAAAGGTCTCGTACCAATGGAGATGTATTCCTTACTTATAAATCCATGATCGTCTCCTTAATCCTTAACAGTACTCTCATTACCTCTTTAAAGGGCAGTAGTGCCCGAAGCGGTTTAGGGTTGAGGGCTTAAGAAGTCGATATTTAGGAAATTTGTGTTCTCAATGGAGGTTTAAGAAGTTTGAATTACGAGATCGAGTTTGAAGGAATTGGTTTTGTGTGGTATTCCCGAGAAACTTATGGTTTTATCTAATAAACTCCAAGTTTAGTTAATTTACGGGATAAACATGGGTTCAACATTGTTAAATTATAGTTTACCATATATATATATATATCATATTTGACCCTTATATTATCGATAAATATCTTGTATGATTGAGGATATTTAATTTTTCATCACGGTTTATCATATGTATCATATTTGACCATTTATTACAGTCAAATATTTAGATATTGGACTCCTATCCTGCTTACTATAAGTATTATATTTGACCCTTTATTACAGTAAAATATTTAGATATTTGACTTTTATCGTGCTTAGTATCATATTTAATCATTTATTACAGTCAAATATTTAGATATTTGACTCTTATCCTGCTCATATTTGACCCTTTATTACAGTAAAATATTTAGATATTTGACTTTTATCGTGCTTAATATCATATTTAATCATTTATTACAGTCAAATATTTAGATATTTTGCTTTGTCATATTTGACTATTTATTACAGTCAAATGTGTAGAGATTTGACTTCTATAGTGCTTTGTCATATTTGACCATTTATTACAGTCAAATATTTAGAGATTTGACTTCTACAGTACTTTATCATATTTGACCATATATTACAGTCAAATATTTTATCGTGTTTACTTATCATATTTGACTCTTTATTACAGTCAAATATTTAGATATTTTTTTTATCGTAACTATCATATGTATCATATTTGTCTTCCTATTATATACAAATATCTGGTATTCATTTATTGTTTTTTTTTCTGTATCCTTAGTAATTTATTTGATTATTTAAATGATTATAAATAGAGAACTTTCATCATCAGTGAAGGTTACTCCTCGATATCGTATATCTAATATGAAAATATTGGGCTTTTACAGGCCGAGAGAGAAGAAGAAGAGGAATTGAAGGTACAAATAGAAACCGCCATTGCACATATGGGTTGAACTTTTTTAAGACTTTGGTCACATGTTTCTGAAGCATTCATTTATCGAAGTTTAGTTCTTCGTTATCAATGTGTTTCCATAATTATTATTAATAAAACTACTCGTGGTATGATTTAGATCTTTGGATTTAGAAGTAAGTTTTCTA

mRNA sequence

ATGGATAACATTCTCGACGAGTATCTGAAATATTGGGAAACGGACATGTTTCTGCTGACGGAAGACTCCGGCAGCGGCGGTTGGGGACTGGAGGAAGCATTTTACGGTGCTTACAATTCGAGCTCGCCGGACGGGAAGGTGTCGCCGTCGTCGCCGTCGGAGGCGTCGAAGAATATCGTATCGGAGAGAAACCGGAGAAAGAAGCTTAACGATAGGCTTATTGCTCTCCGAGCTGTGGTCCCCAATATCACCAAAATGGACAAGGCGTCAATAATAAAAGATGCCATCGTATACATCCAAGAGTTGCGAGCGGAAGAGAAGAGAATCGAAATAGAGATTTCCGATCTGGAATCAAGCGTAAACAACAACGGATCAAGAACCACAAATCAATGGAACGACGAAAACGGAAATCGAATTTGGACGAACATGAAATGGAATCGACAAACAACTGACGCTTCATCGTCTTCCTCGATCGAAGTTCTCGATGTAATTGAGTGTGAATTACGTGGGCGAGAAGGTGATGGTGGTGAGTTTGACGTGCTTACGCCGAGGCAACACGATATTGAAGGTTTGGGAAGTGATTGAATCGTTGAAGTTGAAGATCATTACTGCTAATATCAGTGTCTTCGCAAACAAGCTCTTGAACACGCTCTTCATTGAGGCCGAGAGAGAAGAAGAAGAGGAATTGAAGGTACAAATAGAAACCGCCATTGCACATATGGGTTGAACTTTTTTAAGACTTTGGTCACATGTTTCTGAAGCATTCATTTATCGAAGTTTAGTTCTTCGTTATCAATGTGTTTCCATAATTATTATTAATAAAACTACTCGTGGTATGATTTAGATCTTTGGATTTAGAAGTAAGTTTTCTA

Coding sequence (CDS)

ATGGATAACATTCTCGACGAGTATCTGAAATATTGGGAAACGGACATGTTTCTGCTGACGGAAGACTCCGGCAGCGGCGGTTGGGGACTGGAGGAAGCATTTTACGGTGCTTACAATTCGAGCTCGCCGGACGGGAAGGTGTCGCCGTCGTCGCCGTCGGAGGCGTCGAAGAATATCGTATCGGAGAGAAACCGGAGAAAGAAGCTTAACGATAGGCTTATTGCTCTCCGAGCTGTGGTCCCCAATATCACCAAAATGGACAAGGCGTCAATAATAAAAGATGCCATCGTATACATCCAAGAGTTGCGAGCGGAAGAGAAGAGAATCGAAATAGAGATTTCCGATCTGGAATCAAGCGTAAACAACAACGGATCAAGAACCACAAATCAATGGAACGACGAAAACGGAAATCGAATTTGGACGAACATGAAATGGAATCGACAAACAACTGACGCTTCATCGTCTTCCTCGATCGAAGTTCTCGATGTAATTGAGTGTGAATTACGTGGGCGAGAAGGTGATGGTGGTGAGTTTGACGTGCTTACGCCGAGGCAACACGATATTGAAGGTTTGGGAAGTGATTGA

Protein sequence

MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIVSERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSVNNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDVIECELRGREGDGGEFDVLTPRQHDIEGLGSD

Homology

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BLAST of Carg24192 vs. NCBI nr
Match: KAG7020934.1 (Transcription factor bHLH35, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 377.9 bits (969), Expect = 5.4e-101
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60
           MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV
Sbjct: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60

Query: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSV 120
           SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSV
Sbjct: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSV 120

Query: 121 NNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDVIECELRGREGDGGEFDV 180
           NNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDVIECELRGREGDGGEFDV
Sbjct: 121 NNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDVIECELRGREGDGGEFDV 180

Query: 181 LTPRQHDIEGLGSD 195
           LTPRQHDIEGLGSD
Sbjct: 181 LTPRQHDIEGLGSD 194

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BLAST of Carg24192 vs. NCBI nr
Match: KAG6586111.1 (Transcription factor basic helix-loop-helix 35, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 307.8 bits (787), Expect = 6.8e-80
Identity = 160/163 (98.16%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60
           MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV
Sbjct: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60

Query: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSV 120
           SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLE SV
Sbjct: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLELSV 120

Query: 121 NNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
           NNNGSRTTNQWNDENG+RIWTNMKWNRQTTDASSSSSIEVLD+
Sbjct: 121 NNNGSRTTNQWNDENGDRIWTNMKWNRQTTDASSSSSIEVLDL 163

[Close]

BLAST of Carg24192 vs. NCBI nr
Match: XP_022938316.1 (transcription factor bHLH35-like [Cucurbita moschata])

HSP 1 Score: 305.8 bits (782), Expect = 2.6e-79
Identity = 159/163 (97.55%), Postives = 161/163 (98.77%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60
           MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFY AYNSSSPDGKVSPSSPSEASKNIV
Sbjct: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYSAYNSSSPDGKVSPSSPSEASKNIV 60

Query: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSV 120
           SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIE EISDLESSV
Sbjct: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIETEISDLESSV 120

Query: 121 NNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
           NNNGSRTTNQWNDENG+RIWTNMKWNRQTTDASSSSSIEVLD+
Sbjct: 121 NNNGSRTTNQWNDENGDRIWTNMKWNRQTTDASSSSSIEVLDL 163

[Close]

BLAST of Carg24192 vs. NCBI nr
Match: XP_023538479.1 (transcription factor bHLH35-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 288.9 bits (738), Expect = 3.3e-74
Identity = 150/163 (92.02%), Postives = 158/163 (96.93%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60
           MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSP+GKVSPSSPSEASKNIV
Sbjct: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPEGKVSPSSPSEASKNIV 60

Query: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSV 120
           SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAI YIQELRAEEKRIE EI +LESSV
Sbjct: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIAYIQELRAEEKRIETEIFNLESSV 120

Query: 121 NNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
           NN  SRTTNQWN+ENG++IWTNMKWN+QTTDASSSSSI+VLD+
Sbjct: 121 NNERSRTTNQWNNENGDQIWTNMKWNQQTTDASSSSSIKVLDL 163

[Close]

BLAST of Carg24192 vs. NCBI nr
Match: XP_022965447.1 (transcription factor bHLH35-like [Cucurbita maxima])

HSP 1 Score: 281.2 bits (718), Expect = 6.8e-72
Identity = 149/163 (91.41%), Postives = 156/163 (95.71%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60
           MDNILDEYLK WETDMFLLTEDSGS GWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV
Sbjct: 1   MDNILDEYLKNWETDMFLLTEDSGS-GWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60

Query: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSV 120
           SERNRRKKLNDRLIALRAVVPNIT+MDKASIIKDAI YIQEL+AEEKRI+ EI DLESSV
Sbjct: 61  SERNRRKKLNDRLIALRAVVPNITRMDKASIIKDAITYIQELQAEEKRIKTEIFDLESSV 120

Query: 121 NNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
           NN  SRTTNQWN+ENG+RIWTNMKWN+QTTDASSSSSIEVLD+
Sbjct: 121 NNKRSRTTNQWNNENGDRIWTNMKWNQQTTDASSSSSIEVLDL 162

[Close]

BLAST of Carg24192 vs. ExPASy Swiss-Prot
Match: Q2HIV9 (Transcription factor bHLH35 OS=Arabidopsis thaliana OX=3702 GN=BHLH35 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.8e-28
Identity = 87/165 (52.73%), Postives = 110/165 (66.67%), Query Frame = 0


Query: 1   MDNILDEYL-KYWETDMFLLTED-SGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKN 60
           M++I+D+ L  YWE   FL  ED      W LEEA  G+Y+SSSPDG  S    S ASKN
Sbjct: 1   MEDIVDQELSNYWEPSSFLQNEDFEYDRSWPLEEAISGSYDSSSPDGAAS----SPASKN 60

Query: 61  IVSERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLES 120
           IVSERNRR+KLN RL ALR+VVPNITKMDKASIIKDAI YI+ L+ EEK++E EI +LES
Sbjct: 61  IVSERNRRQKLNQRLFALRSVVPNITKMDKASIIKDAISYIEGLQYEEKKLEAEIRELES 120

Query: 121 SVNNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
           +  ++ S + +   D     +    K  +Q    SS+S IEVL++
Sbjct: 121 TPKSSLSFSKDFDRD---LLVPVTSKKMKQLDSGSSTSLIEVLEL 158

[Close]

BLAST of Carg24192 vs. ExPASy Swiss-Prot
Match: Q700E3 (Transcription factor bHLH27 OS=Arabidopsis thaliana OX=3702 GN=BHLH27 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.5e-26
Identity = 67/118 (56.78%), Postives = 89/118 (75.42%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60
           M+++  EY  YWET MF   ++     W +EEAF G+  SSSPDG  + +SP+ +SKN+V
Sbjct: 1   MEDLDHEYKNYWETTMFFQNQELEFDSWPMEEAFSGSGESSSPDG--AATSPA-SSKNVV 60

Query: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLES 119
           SERNRR+KLN RL ALR+VVPNI+K+DKAS+IKD+I Y+QEL  +EK +E EI +LES
Sbjct: 61  SERNRRQKLNQRLFALRSVVPNISKLDKASVIKDSIDYMQELIDQEKTLEAEIRELES 115

[Close]

BLAST of Carg24192 vs. ExPASy Swiss-Prot
Match: Q0JEB7 (Transcription factor BHLH6 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH6 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.1e-16
Identity = 62/149 (41.61%), Postives = 83/149 (55.70%), Query Frame = 0


Query: 6   DEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSE----------- 65
           + +  YWET  +L +E+  S    L       Y SSSPDG  S S+P+            
Sbjct: 9   ESFAYYWETQRYLESEELDS--MYLPTQDDSNYESSSPDGSHSSSAPAPAAVGGDAAAAV 68

Query: 66  ------------------------ASKNIVSERNRRKKLNDRLIALRAVVPNITKMDKAS 120
                                   A+KNI+ ER+RR+KLN++L ALR+VVPNITKMDKAS
Sbjct: 69  AGSGGGMTTMMMGGGGGGGDDAGGANKNILMERDRRRKLNEKLYALRSVVPNITKMDKAS 128

[Close]

BLAST of Carg24192 vs. ExPASy Swiss-Prot
Match: Q0V7X4 (Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR OS=Arabidopsis thaliana OX=3702 GN=FIT PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.8e-13
Identity = 42/102 (41.18%), Postives = 74/102 (72.55%), Query Frame = 0


Query: 25  SGGWGLEEAFYGAYNSS--SPDGKVSPSSPSEASKNIVSERNRRKKLNDRLIALRAVVPN 84
           +GG   EE +    +SS  + +   +  + ++ S+ ++SER RR ++ D+L ALR++VPN
Sbjct: 97  AGGEEDEEDYNDGDDSSATTTNNDGTRKTKTDRSRTLISERRRRGRMKDKLYALRSLVPN 156

Query: 85  ITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSVNNNG 125
           ITKMDKASI+ DA++Y+QEL+++ K+++ +I+ LE+S+N+ G
Sbjct: 157 ITKMDKASIVGDAVLYVQELQSQAKKLKSDIAGLEASLNSTG 198

[Close]

BLAST of Carg24192 vs. ExPASy Swiss-Prot
Match: A0A0N7KIY3 (Transcription factor BHLH156 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH156 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.4e-11
Identity = 35/66 (53.03%), Postives = 53/66 (80.30%), Query Frame = 0


Query: 54  EASKNIVSERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEI 113
           + SK IVSER RR ++ ++L  LRA+VPNITKMDKASII DA+VY+++L+A  ++++ E+
Sbjct: 131 DRSKTIVSERKRRVRMKEKLYELRALVPNITKMDKASIIADAVVYVKDLQAHARKLKEEV 190

Query: 114 SDLESS 120
           + LE +
Sbjct: 191 AALEEA 196

[Close]

BLAST of Carg24192 vs. ExPASy TrEMBL
Match: A0A6J1FCT5 (transcription factor bHLH35-like OS=Cucurbita moschata OX=3662 GN=LOC111444452 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.3e-79
Identity = 159/163 (97.55%), Postives = 161/163 (98.77%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60
           MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFY AYNSSSPDGKVSPSSPSEASKNIV
Sbjct: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYSAYNSSSPDGKVSPSSPSEASKNIV 60

Query: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSV 120
           SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIE EISDLESSV
Sbjct: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIETEISDLESSV 120

Query: 121 NNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
           NNNGSRTTNQWNDENG+RIWTNMKWNRQTTDASSSSSIEVLD+
Sbjct: 121 NNNGSRTTNQWNDENGDRIWTNMKWNRQTTDASSSSSIEVLDL 163

[Close]

BLAST of Carg24192 vs. ExPASy TrEMBL
Match: A0A6J1HKC8 (transcription factor bHLH35-like OS=Cucurbita maxima OX=3661 GN=LOC111465351 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 3.3e-72
Identity = 149/163 (91.41%), Postives = 156/163 (95.71%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60
           MDNILDEYLK WETDMFLLTEDSGS GWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV
Sbjct: 1   MDNILDEYLKNWETDMFLLTEDSGS-GWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60

Query: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSV 120
           SERNRRKKLNDRLIALRAVVPNIT+MDKASIIKDAI YIQEL+AEEKRI+ EI DLESSV
Sbjct: 61  SERNRRKKLNDRLIALRAVVPNITRMDKASIIKDAITYIQELQAEEKRIKTEIFDLESSV 120

Query: 121 NNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
           NN  SRTTNQWN+ENG+RIWTNMKWN+QTTDASSSSSIEVLD+
Sbjct: 121 NNKRSRTTNQWNNENGDRIWTNMKWNQQTTDASSSSSIEVLDL 162

[Close]

BLAST of Carg24192 vs. ExPASy TrEMBL
Match: A0A6J1EPK8 (transcription factor bHLH35-like OS=Cucurbita moschata OX=3662 GN=LOC111436215 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 9.7e-40
Identity = 110/163 (67.48%), Postives = 124/163 (76.07%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60
           M+NILDEY  YWET+MFL TE+  S  WG++EAFYG+Y+SSSPDG  +PSS  EASKNI+
Sbjct: 1   MENILDEYQYYWETNMFLQTEEFDS--WGVDEAFYGSYDSSSPDG--TPSS-LEASKNIM 60

Query: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSV 120
           SERNRRKKLNDRL+ALRAVVPNITKMDKASIIKDAI YIQELRAEEKRIE EISDLES  
Sbjct: 61  SERNRRKKLNDRLLALRAVVPNITKMDKASIIKDAIEYIQELRAEEKRIEAEISDLESGK 120

Query: 121 NNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
             N  R TN+  D +G         NRQ  +   S  IEVLD+
Sbjct: 121 IKN--RATNE--DYDGILREKRTNKNRQVPEPPPSFPIEVLDL 154

[Close]

BLAST of Carg24192 vs. ExPASy TrEMBL
Match: A0A5D3BTM5 (Transcription factor bHLH35-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001740 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 6.3e-39
Identity = 106/168 (63.10%), Postives = 125/168 (74.40%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTED-SGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNI 60
           M+NILDEY  YWET+MFL TE+   +  WGL+EAFYG+Y+SSSPDG +   S  EASKNI
Sbjct: 1   MENILDEYRYYWETNMFLQTEEFDRNFSWGLDEAFYGSYDSSSPDGTM---SSLEASKNI 60

Query: 61  VSERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESS 120
           +SERNRRKKLNDRL+ALRAVVPNITKMDKASIIKDAI YIQELR+EE RIE EIS+LES 
Sbjct: 61  LSERNRRKKLNDRLLALRAVVPNITKMDKASIIKDAIEYIQELRSEENRIETEISNLES- 120

Query: 121 VNNNGSRTTNQWNDENGNRIWTNMKW----NRQTTDASSSSSIEVLDV 164
                S T+N+ +D   N   T  +     NRQ  +  +SS IE+LDV
Sbjct: 121 -GKFKSTTSNEDDDRENNTRNTRKRGTKNSNRQLREKPTSSPIEILDV 163

[Close]

BLAST of Carg24192 vs. ExPASy TrEMBL
Match: A0A6J1K7A8 (transcription factor bHLH35-like OS=Cucurbita maxima OX=3661 GN=LOC111491799 PE=4 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 8.2e-39
Identity = 108/163 (66.26%), Postives = 124/163 (76.07%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60
           M+NILDEY  YWET+MFL TE+  S  WG++EAFYG+Y+SSSPDG  +PSS  EASKNI+
Sbjct: 1   MENILDEYQYYWETNMFLQTEEFDS--WGVDEAFYGSYDSSSPDG--TPSS-LEASKNIL 60

Query: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESSV 120
           SERNRRKKLNDRL+ALRAVVPNITKMDKASIIKDAI YIQELRA+EKRIE EISDLES  
Sbjct: 61  SERNRRKKLNDRLLALRAVVPNITKMDKASIIKDAIEYIQELRAQEKRIEEEISDLESGK 120

Query: 121 NNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
             N  R TN+  D++G         N Q  +   S  IEVLD+
Sbjct: 121 IKN--RATNE--DDDGILREKRTNKNGQVPEPPPSFPIEVLDL 154

[Close]

BLAST of Carg24192 vs. TAIR 10
Match: AT5G57150.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.4e-30
Identity = 87/164 (53.05%), Postives = 110/164 (67.07%), Query Frame = 0


Query: 1   MDNILDEYL-KYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNI 60
           M++I+D+ L  YWE   FL  ED     W LEEA  G+Y+SSSPDG  S    S ASKNI
Sbjct: 1   MEDIVDQELSNYWEPSSFLQNEDFEYDSWPLEEAISGSYDSSSPDGAAS----SPASKNI 60

Query: 61  VSERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLESS 120
           VSERNRR+KLN RL ALR+VVPNITKMDKASIIKDAI YI+ L+ EEK++E EI +LES+
Sbjct: 61  VSERNRRQKLNQRLFALRSVVPNITKMDKASIIKDAISYIEGLQYEEKKLEAEIRELEST 120

Query: 121 VNNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
             ++ S + +   D     +    K  +Q    SS+S IEVL++
Sbjct: 121 PKSSLSFSKDFDRD---LLVPVTSKKMKQLDSGSSTSLIEVLEL 157

[Close]

BLAST of Carg24192 vs. TAIR 10
Match: AT5G57150.3 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-29
Identity = 87/165 (52.73%), Postives = 110/165 (66.67%), Query Frame = 0


Query: 1   MDNILDEYL-KYWETDMFLLTED-SGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKN 60
           M++I+D+ L  YWE   FL  ED      W LEEA  G+Y+SSSPDG  S    S ASKN
Sbjct: 1   MEDIVDQELSNYWEPSSFLQNEDFEYDRSWPLEEAISGSYDSSSPDGAAS----SPASKN 60

Query: 61  IVSERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLES 120
           IVSERNRR+KLN RL ALR+VVPNITKMDKASIIKDAI YI+ L+ EEK++E EI +LES
Sbjct: 61  IVSERNRRQKLNQRLFALRSVVPNITKMDKASIIKDAISYIEGLQYEEKKLEAEIRELES 120

Query: 121 SVNNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
           +  ++ S + +   D     +    K  +Q    SS+S IEVL++
Sbjct: 121 TPKSSLSFSKDFDRD---LLVPVTSKKMKQLDSGSSTSLIEVLEL 158

[Close]

BLAST of Carg24192 vs. TAIR 10
Match: AT5G57150.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-29
Identity = 87/165 (52.73%), Postives = 110/165 (66.67%), Query Frame = 0


Query: 1   MDNILDEYL-KYWETDMFLLTED-SGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKN 60
           M++I+D+ L  YWE   FL  ED      W LEEA  G+Y+SSSPDG  S    S ASKN
Sbjct: 1   MEDIVDQELSNYWEPSSFLQNEDFEYDRSWPLEEAISGSYDSSSPDGAAS----SPASKN 60

Query: 61  IVSERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLES 120
           IVSERNRR+KLN RL ALR+VVPNITKMDKASIIKDAI YI+ L+ EEK++E EI +LES
Sbjct: 61  IVSERNRRQKLNQRLFALRSVVPNITKMDKASIIKDAISYIEGLQYEEKKLEAEIRELES 120

Query: 121 SVNNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
           +  ++ S + +   D     +    K  +Q    SS+S IEVL++
Sbjct: 121 TPKSSLSFSKDFDRD---LLVPVTSKKMKQLDSGSSTSLIEVLEL 158

[Close]

BLAST of Carg24192 vs. TAIR 10
Match: AT5G57150.4 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-29
Identity = 87/165 (52.73%), Postives = 110/165 (66.67%), Query Frame = 0


Query: 1   MDNILDEYL-KYWETDMFLLTED-SGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKN 60
           M++I+D+ L  YWE   FL  ED      W LEEA  G+Y+SSSPDG  S    S ASKN
Sbjct: 1   MEDIVDQELSNYWEPSSFLQNEDFEYDRSWPLEEAISGSYDSSSPDGAAS----SPASKN 60

Query: 61  IVSERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLES 120
           IVSERNRR+KLN RL ALR+VVPNITKMDKASIIKDAI YI+ L+ EEK++E EI +LES
Sbjct: 61  IVSERNRRQKLNQRLFALRSVVPNITKMDKASIIKDAISYIEGLQYEEKKLEAEIRELES 120

Query: 121 SVNNNGSRTTNQWNDENGNRIWTNMKWNRQTTDASSSSSIEVLDV 164
           +  ++ S + +   D     +    K  +Q    SS+S IEVL++
Sbjct: 121 TPKSSLSFSKDFDRD---LLVPVTSKKMKQLDSGSSTSLIEVLEL 158

[Close]

BLAST of Carg24192 vs. TAIR 10
Match: AT4G29930.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 3.2e-27
Identity = 67/118 (56.78%), Postives = 89/118 (75.42%), Query Frame = 0


Query: 1   MDNILDEYLKYWETDMFLLTEDSGSGGWGLEEAFYGAYNSSSPDGKVSPSSPSEASKNIV 60
           M+++  EY  YWET MF   ++     W +EEAF G+  SSSPDG  + +SP+ +SKN+V
Sbjct: 1   MEDLDHEYKNYWETTMFFQNQELEFDSWPMEEAFSGSGESSSPDG--AATSPA-SSKNVV 60

Query: 61  SERNRRKKLNDRLIALRAVVPNITKMDKASIIKDAIVYIQELRAEEKRIEIEISDLES 119
           SERNRR+KLN RL ALR+VVPNI+K+DKAS+IKD+I Y+QEL  +EK +E EI +LES
Sbjct: 61  SERNRRQKLNQRLFALRSVVPNISKLDKASVIKDSIDYMQELIDQEKTLEAEIRELES 115

The following BLAST results are available for this feature:

BLAST of Carg24192 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020934.1	5.4e-101	100.00	Transcription factor bHLH35, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
KAG6586111.1	6.8e-80	98.16	Transcription factor basic helix-loop-helix 35, partial [Cucurbita argyrosperma ...	[more]
XP_022938316.1	2.6e-79	97.55	transcription factor bHLH35-like [Cucurbita moschata]	[more]
XP_023538479.1	3.3e-74	92.02	transcription factor bHLH35-like [Cucurbita pepo subsp. pepo]	[more]
XP_022965447.1	6.8e-72	91.41	transcription factor bHLH35-like [Cucurbita maxima]	[more]

BLAST of Carg24192 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2HIV9	2.8e-28	52.73	Transcription factor bHLH35 OS=Arabidopsis thaliana OX=3702 GN=BHLH35 PE=2 SV=1	[more]
Q700E3	4.5e-26	56.78	Transcription factor bHLH27 OS=Arabidopsis thaliana OX=3702 GN=BHLH27 PE=2 SV=1	[more]
Q0JEB7	1.1e-16	41.61	Transcription factor BHLH6 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH6 PE=...	[more]
Q0V7X4	9.8e-13	41.18	Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR OS=Ar...	[more]
A0A0N7KIY3	2.4e-11	53.03	Transcription factor BHLH156 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH156...	[more]

BLAST of Carg24192 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FCT5	1.3e-79	97.55	transcription factor bHLH35-like OS=Cucurbita moschata OX=3662 GN=LOC111444452 P...	[more]
A0A6J1HKC8	3.3e-72	91.41	transcription factor bHLH35-like OS=Cucurbita maxima OX=3661 GN=LOC111465351 PE=...	[more]
A0A6J1EPK8	9.7e-40	67.48	transcription factor bHLH35-like OS=Cucurbita moschata OX=3662 GN=LOC111436215 P...	[more]
A0A5D3BTM5	6.3e-39	63.10	Transcription factor bHLH35-like isoform X1 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A6J1K7A8	8.2e-39	66.26	transcription factor bHLH35-like OS=Cucurbita maxima OX=3661 GN=LOC111491799 PE=...	[more]

BLAST of Carg24192 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G57150.1	1.4e-30	53.05	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT5G57150.3	2.0e-29	52.73	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT5G57150.2	2.0e-29	52.73	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT5G57150.4	2.0e-29	52.73	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT4G29930.1	3.2e-27	56.78	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 99..119
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 172..194
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 41..58
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 41..62
None	No IPR available	PANTHER	PTHR31945:SF26	TRANSCRIPTION FACTOR BHLH35	coord: 2..167
None	No IPR available	PANTHER	PTHR31945	TRANSCRIPTION FACTOR SCREAM2-RELATED	coord: 2..167
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	SMART	SM00353	finulus	coord: 59..108 e-value: 3.8E-13 score: 59.7
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 60..102 e-value: 6.4E-10 score: 38.9
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 53..102 score: 15.751427
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	4.10.280.10		coord: 48..119 e-value: 1.2E-15 score: 59.1
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 52..117

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24192-RA	Carg24192-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046983	protein dimerization activity