Carg24187 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24187
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionL-gulonolactone oxidase
LocationCarg_Chr06: 6142640 .. 6146876 (-)
RNA-Seq ExpressionCarg24187
SyntenyCarg24187
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGAGCCGCCCACAACAACGCGGTCCACGGACCCTGGACTGACCACTATGCGGTCGTCTAATGCCTTCCGGTTCACTACCTTCATCTTCTTTCTAGTTCTTCTTGTGTCAGTGTCCGAGTCTACTCCGCCGGAGGACCCAATCAAATGCCGATCCCCAAACGCCAACTGCACCATTACAAACGCCTACGCCACCTTCCCAGATCGGGCCATCTGCCGGGTGGGAGAGGTGAAGTACCCAACCACAGAGGCGGAGGTGATCTCCGCGGTGGCGGAGGCCACGAGAGCCATGAGGAAAATTAAGGTGGGCACTCGATTTTACCACAGCATTCCGAAGCTGGCATGCCCAGATGGGGTTGATGGGGTGGTGATAAGCACCAAGTATTTGAACCGGGTTTTGAAGGTGGATGGGGAGGGCATGAGTATAACCGTGGAGGGTGGAGTGACGGTGAGGGAGATCATAGAGGTGGCTGCGGCGGGTGGGCTTGCGTTGCCGCATGCGCCGTATTGGTGGGGCCTCACAATTGGTGGGCTGCTGAGTACGGGGGCACATGGCAGCTCGTTGTGGGGCAAAGGGAGTGCTGTTCATGATTACGTTGTGGGTATTAAAATGGTCAGTCCTGGAGGAGCTCAAGATGGGTACGCCAAAGTTCGGAGGCTTGTTGATGGCGATCAACACCTCAACGCTGCCAAAGTTTCATTGGGAGTTCTTGGAATTATTACACAGGTGATGATCCTCTTCTTCTTAGATCATCAAAAATTCCTTTTTAAATAAAATTAAAGAAAAAAAAATTATAGATTTTTCTCAATTAATTTATCATAAACTATTTTTCCTTCGTTACTAATTACAAATTTATAGGTCGGCTTAATTATTGTACTTGATTGTATTGTAGTCTTATACCGTAAGAAAAGTGAGGTGACTCATCAAATTCATAAAATAAAAAGTAAATTTCGAGTTGTTTTTTTCGTGACATAATTGTTATAATTTTTTCATTGATTTGGTAGGGAAGTGGAAGATTTTTTTGTTATTATTTATTTATTTATTTATTATGTATGTAATAATTAAAGGAAGAAAGAAAGTTGCTGTAAGTTTTTTTTTGGAAAAGTCGGAAATAAATACAAATTTTGGACTATAAATACTAAATTGCCCTTTAAAGACAAAATAACTATAAATTTAAAATCTTTAAGATGAAAATGAAATTTAATTTTTAATAAAAGTACAAGAACTAAATTAGTTGATTTTATCAAAATATTTCTTAAATTAACCCTTTAATATTTCACTTGTTAAAAGATATTAAATTAAATAAATTGAAAATTTTTATTTAGGTTATATTAATAAAAAAAAACTCTAAATTTTAAATTTTGTCTAAAAATAGTGGAGGGTCTAAACTTAAAAGAAAAAAAAATTAAAATATTTTTACCATTTAAAAATATTAAAAAATGTCAAATTTTTATTTCAAAAATACTAAAACTTTTAAAAGAGCTTAAAAATCACTTTGAATTTCTTAAATTAAAAAAAGTTTCAAGGACCTTAAAAAAATTAAAAATTAAAACTCCCTTAAATATCCGGTTTTAAAAATAATAGTATCCATAAAATTGAAGAGGAAAATGGGATTTTAATAAATGATTTCTATATATATACTGAGGTAATAATTGTTTAAAACATTTTTTTTTATAAATGTTAAAGAGCGTTAATATTACTTTCCAATGTTTATAGATATATTTTTAAATTTTTTAAAAAATGGAAGAATATTAATGAAACTTGTTAACAAAATACTAATAATTTTTATCCAAATCTAACAATAGGGATATAAAACTTTTTTTTTTGTTTAAATTCATCAGTATTATTGAAACTTTTGAATAATACTTTTGAAAATTCAATAGTATTTTTGTCACAAGTATTTTTATTAATTTAGAGTCAGTATTTCCAAAAACGGCATATATTTGGCAATATTAATTGATGAGGAGTGAGTGTGTAGGTCACTCTGAAATTGCAACCCATGTTCAAGCGATCCATCACCTATTTTGAAAGGGATGACACTGATTTGAGCGATCAAGTGATTAGCTTCGGTAGAAGCTACGAGTTTGCAGACATGACTTGGTATCCAAGCCAGCGAAAAGTTGTGTATCGCAAAGACACCCGCGTTTCATCCAATACATCCGGCAATGGCTTGTATGATTTTATTCCATTTCGTCCTACACCTTCAGCTGCCTTGGCCATTGTTCGAGCTTCAGGTCAGTGTAGATCTACGTGTTATAAGTTGTCATGAAAAATAAGTTCATGCATTTCATACGCATGTGTTTACCATTTATTTTAGTTCATCTTCCATACCATTTATTTTAGTTCATCTTCCATTTTGATATATGATCATCTTAATTTATGTAGTATAGATCTACACGTTATAAGTTGTCATGAAAAATAGGTTTATACATGTATTGTAATTTATTTTGGTTCATAACTCACATTTATTTGATTCATCTATCGTTTGGTTCATAATTCTCATTTATTTGGTTCATAACCCTATTTAGTTCATCCTCCAATCATATATAAAAACTTGTCTACTGTCATTTTGTATTTAACTAGAGACAAGTAGGAGAAACTTTGTGTTGAGAATTGTTAGGAGGGAGTCTCATGTTCGCTAATTTAGGGAATGATCATGTGTTTATAAGTAAGAATACTATCTTCATTGCTACGAGGCTTTGTACCAAACCTAAAGCAGAACTACGAGAGTTTATTTTCAAAGTGGACAATATCATAAATTGTGGAGAGTCATAGAATTTAGGGGGATCTCTTCTCTTTAAACTATGTATATCTTTATGTCTCCTTGTCTACTTTTTGTTTGTGGGTGTACAAGTGCGATCGATTTTGCAACACTACAAGCCCCCCCGACCCTTGTACCTTAATTACCCACTTCAAGTTTTGACATCTTGTTTTACATAGAGGAGTACCAAGAAGCAAGGCGTGATGCAGCTGGGAAGTGTCTTAGTGCAAAGATTATCACGTCACTTCTCTCACTATCAGCGTACGGATTAACCAACAATGGTAAGAACACATACTAAAGACCATTACAAGATTCAAAATTCACTTAATTAACCGCATGCAATTATTTGAATAGGTATCACATTTCTCAAATATCCAGTCATCGGGTATAACAATCGCCTTCAATCCTCTGGATCTTGCCTAGACAGTGTCAATGATCTTAGAATCACAGCATGTCCTTGGGAGCCATCGATAAAAGGGGAGTTTTTCCACGAAATAGGCATCAGCATTGACTTAAAAGTGGTGAAAGGTTTCATAGAAGACGTGAGAAAGCTTGGTGAGTTAGAGCCCATGGCGTTTTGTGGCCTTGAGCTCTACAATGGCATATTGATGCGCTATGTCAAAGCCTCCACGGCCTACTTGGGGAAGGAAGAAGATGGCATCAACTTTGACTTCACATATTATAGGAGCAAAGACCCAATGAGTCCAAGGCTTTTTGAAGACGTGCTTGAAGAGGTGGAGCAAATGGCGTTGTTCAAATATGGAGGTAGTCCGCATTGGGGGAAGAACAGGAACATTGCATTTCTCAAAGCGATTGGGAAGTACAAAAATAGAGAAGGGTTTTTGAAGATTAAGAATGAATATGATCCGTGCGGGCTGTTCTCAAATGAATGGACCAATCAAATTCTTGGAGTGGAAGGAACTGTGAGTATAGATAAAGATGGGTGTGCTTTGGAAGGATTGTGTTTATGCTCTAAAGATAGTCATTGTGCCCCAAGTAAGGGCTACTTTTGTAGGGCTGGCAGGATTTATAAGGAAGCTAGAGTTTGTTCTTATTTAGGATTCAAATCTTAGTCAACTTGTAAGGTCGTTATAACTTTAATATTTCTTCGTATCTGTTTGTTATTTCAGACCTGATTACGTCGTGGACTGTTTTGAACTATTGCCTTTTAGGAGCTATCTACACTATCGACATGCTCCTCTATCTAGGCAATAGAGCCCGTCAAACTATCTCCAATCTTGAACATCAGTTCTTGGAAGGGCATACCATTTTCGCAGCTAGCTACTATGTCAATCGTGTTGGGCTTGGCAACAAGCTCTTTTATGCACTCGTCTAAGTACTGTAGGTTGTCATGGACTTCTTGCGAATATTGCATTTGTTCTTTGACGTTGACCAATTTGTTGTGTGTAGAATGTGTTGAATTTGTTTGCAAATGACTTGTCTTGGATGACATCTTAATGAATGCAAGAAAAGTTC

mRNA sequence

AAGGAGCCGCCCACAACAACGCGGTCCACGGACCCTGGACTGACCACTATGCGGTCGTCTAATGCCTTCCGGTTCACTACCTTCATCTTCTTTCTAGTTCTTCTTGTGTCAGTGTCCGAGTCTACTCCGCCGGAGGACCCAATCAAATGCCGATCCCCAAACGCCAACTGCACCATTACAAACGCCTACGCCACCTTCCCAGATCGGGCCATCTGCCGGGTGGGAGAGGTGAAGTACCCAACCACAGAGGCGGAGGTGATCTCCGCGGTGGCGGAGGCCACGAGAGCCATGAGGAAAATTAAGGTGGGCACTCGATTTTACCACAGCATTCCGAAGCTGGCATGCCCAGATGGGGTTGATGGGGTGGTGATAAGCACCAAGTATTTGAACCGGGTTTTGAAGGTGGATGGGGAGGGCATGAGTATAACCGTGGAGGGTGGAGTGACGGTGAGGGAGATCATAGAGGTGGCTGCGGCGGGTGGGCTTGCGTTGCCGCATGCGCCGTATTGGTGGGGCCTCACAATTGGTGGGCTGCTGAGTACGGGGGCACATGGCAGCTCGTTGTGGGGCAAAGGGAGTGCTGTTCATGATTACGTTGTGGGTATTAAAATGGTCAGTCCTGGAGGAGCTCAAGATGGGTACGCCAAAGTTCGGAGGCTTGTTGATGGCGATCAACACCTCAACGCTGCCAAAGTTTCATTGGGAGTTCTTGGAATTATTACACAGGTCACTCTGAAATTGCAACCCATGTTCAAGCGATCCATCACCTATTTTGAAAGGGATGACACTGATTTGAGCGATCAAGTGATTAGCTTCGGTAGAAGCTACGAGTTTGCAGACATGACTTGGTATCCAAGCCAGCGAAAAGTTGTGTATCGCAAAGACACCCGCGTTTCATCCAATACATCCGGCAATGGCTTGTATGATTTTATTCCATTTCAGGAGTACCAAGAAGCAAGGCGTGATGCAGCTGGGAAGTGTCTTAGTGCAAAGATTATCACGTCACTTCTCTCACTATCAGCGTACGGATTAACCAACAATGGTATCACATTTCTCAAATATCCAGTCATCGGGTATAACAATCGCCTTCAATCCTCTGGATCTTGCCTAGACAGTGTCAATGATCTTAGAATCACAGCATGTCCTTGGGAGCCATCGATAAAAGGGGAGTTTTTCCACGAAATAGGCATCAGCATTGACTTAAAAGTGGTGAAAGGTTTCATAGAAGACGTGAGAAAGCTTGGTGAGTTAGAGCCCATGGCGTTTTGTGGCCTTGAGCTCTACAATGGCATATTGATGCGCTATGTCAAAGCCTCCACGGCCTACTTGGGGAAGGAAGAAGATGGCATCAACTTTGACTTCACATATTATAGGAGCAAAGACCCAATGAGTCCAAGGCTTTTTGAAGACGTGCTTGAAGAGGTGGAGCAAATGGCGTTGTTCAAATATGGAGGTAGTCCGCATTGGGGGAAGAACAGGAACATTGCATTTCTCAAAGCGATTGGGAAGTACAAAAATAGAGAAGGGTTTTTGAAGATTAAGAATGAATATGATCCGTGCGGGCTGTTCTCAAATGAATGGACCAATCAAATTCTTGGAGTGGAAGGAACTGTGAGTATAGATAAAGATGGGTGTGCTTTGGAAGGATTGTGTTTATGCTCTAAAGATAGTCATTGTGCCCCAAGTAAGGGCTACTTTTGTAGGGCTGGCAGGATTTATAAGGAAGCTAGAGTTTGTTCTTATTTAGGATTCAAATCTTAGTCAACTTGTAAGGTCGTTATAACTTTAATATTTCTTCGTATCTGTTTGTTATTTCAGACCTGATTACGTCGTGGACTGTTTTGAACTATTGCCTTTTAGGAGCTATCTACACTATCGACATGCTCCTCTATCTAGGCAATAGAGCCCGTCAAACTATCTCCAATCTTGAACATCAGTTCTTGGAAGGGCATACCATTTTCGCAGCTAGCTACTATGTCAATCGTGTTGGGCTTGGCAACAAGCTCTTTTATGCACTCGTCTAAGTACTGTAGGTTGTCATGGACTTCTTGCGAATATTGCATTTGTTCTTTGACGTTGACCAATTTGTTGTGTGTAGAATGTGTTGAATTTGTTTGCAAATGACTTGTCTTGGATGACATCTTAATGAATGCAAGAAAAGTTC

Coding sequence (CDS)

AAGGAGCCGCCCACAACAACGCGGTCCACGGACCCTGGACTGACCACTATGCGGTCGTCTAATGCCTTCCGGTTCACTACCTTCATCTTCTTTCTAGTTCTTCTTGTGTCAGTGTCCGAGTCTACTCCGCCGGAGGACCCAATCAAATGCCGATCCCCAAACGCCAACTGCACCATTACAAACGCCTACGCCACCTTCCCAGATCGGGCCATCTGCCGGGTGGGAGAGGTGAAGTACCCAACCACAGAGGCGGAGGTGATCTCCGCGGTGGCGGAGGCCACGAGAGCCATGAGGAAAATTAAGGTGGGCACTCGATTTTACCACAGCATTCCGAAGCTGGCATGCCCAGATGGGGTTGATGGGGTGGTGATAAGCACCAAGTATTTGAACCGGGTTTTGAAGGTGGATGGGGAGGGCATGAGTATAACCGTGGAGGGTGGAGTGACGGTGAGGGAGATCATAGAGGTGGCTGCGGCGGGTGGGCTTGCGTTGCCGCATGCGCCGTATTGGTGGGGCCTCACAATTGGTGGGCTGCTGAGTACGGGGGCACATGGCAGCTCGTTGTGGGGCAAAGGGAGTGCTGTTCATGATTACGTTGTGGGTATTAAAATGGTCAGTCCTGGAGGAGCTCAAGATGGGTACGCCAAAGTTCGGAGGCTTGTTGATGGCGATCAACACCTCAACGCTGCCAAAGTTTCATTGGGAGTTCTTGGAATTATTACACAGGTCACTCTGAAATTGCAACCCATGTTCAAGCGATCCATCACCTATTTTGAAAGGGATGACACTGATTTGAGCGATCAAGTGATTAGCTTCGGTAGAAGCTACGAGTTTGCAGACATGACTTGGTATCCAAGCCAGCGAAAAGTTGTGTATCGCAAAGACACCCGCGTTTCATCCAATACATCCGGCAATGGCTTGTATGATTTTATTCCATTTCAGGAGTACCAAGAAGCAAGGCGTGATGCAGCTGGGAAGTGTCTTAGTGCAAAGATTATCACGTCACTTCTCTCACTATCAGCGTACGGATTAACCAACAATGGTATCACATTTCTCAAATATCCAGTCATCGGGTATAACAATCGCCTTCAATCCTCTGGATCTTGCCTAGACAGTGTCAATGATCTTAGAATCACAGCATGTCCTTGGGAGCCATCGATAAAAGGGGAGTTTTTCCACGAAATAGGCATCAGCATTGACTTAAAAGTGGTGAAAGGTTTCATAGAAGACGTGAGAAAGCTTGGTGAGTTAGAGCCCATGGCGTTTTGTGGCCTTGAGCTCTACAATGGCATATTGATGCGCTATGTCAAAGCCTCCACGGCCTACTTGGGGAAGGAAGAAGATGGCATCAACTTTGACTTCACATATTATAGGAGCAAAGACCCAATGAGTCCAAGGCTTTTTGAAGACGTGCTTGAAGAGGTGGAGCAAATGGCGTTGTTCAAATATGGAGGTAGTCCGCATTGGGGGAAGAACAGGAACATTGCATTTCTCAAAGCGATTGGGAAGTACAAAAATAGAGAAGGGTTTTTGAAGATTAAGAATGAATATGATCCGTGCGGGCTGTTCTCAAATGAATGGACCAATCAAATTCTTGGAGTGGAAGGAACTGTGAGTATAGATAAAGATGGGTGTGCTTTGGAAGGATTGTGTTTATGCTCTAAAGATAGTCATTGTGCCCCAAGTAAGGGCTACTTTTGTAGGGCTGGCAGGATTTATAAGGAAGCTAGAGTTTGTTCTTATTTAGGATTCAAATCTTAG

Protein sequence

KEPPTTTRSTDPGLTTMRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPFQEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFKS
Homology
BLAST of Carg24187 vs. NCBI nr
Match: KAG7028488.1 (putative L-gulonolactone oxidase 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1197.2 bits (3096), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0

Query: 1   KEPPTTTRSTDPGLTTMRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTIT 60
           KEPPTTTRSTDPGLTTMRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTIT
Sbjct: 1   KEPPTTTRSTDPGLTTMRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTIT 60

Query: 61  NAYATFPDRAICRVGEVKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVD 120
           NAYATFPDRAICRVGEVKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVD
Sbjct: 61  NAYATFPDRAICRVGEVKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVD 120

Query: 121 GVVISTKYLNRVLKVDGEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLS 180
           GVVISTKYLNRVLKVDGEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLS
Sbjct: 121 GVVISTKYLNRVLKVDGEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLS 180

Query: 181 TGAHGSSLWGKGSAVHDYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGII 240
           TGAHGSSLWGKGSAVHDYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGII
Sbjct: 181 TGAHGSSLWGKGSAVHDYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGII 240

Query: 241 TQVTLKLQPMFKRSITYFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSS 300
           TQVTLKLQPMFKRSITYFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSS
Sbjct: 241 TQVTLKLQPMFKRSITYFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSS 300

Query: 301 NTSGNGLYDFIPFQEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYN 360
           NTSGNGLYDFIPFQEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYN
Sbjct: 301 NTSGNGLYDFIPFQEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYN 360

Query: 361 NRLQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPM 420
           NRLQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPM
Sbjct: 361 NRLQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPM 420

Query: 421 AFCGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMAL 480
           AFCGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMAL
Sbjct: 421 AFCGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMAL 480

Query: 481 FKYGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSI 540
           FKYGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSI
Sbjct: 481 FKYGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSI 540

Query: 541 DKDGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFKS 587
           DKDGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFKS
Sbjct: 541 DKDGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFKS 586

BLAST of Carg24187 vs. NCBI nr
Match: XP_023540226.1 (probable L-gulonolactone oxidase 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 561/583 (96.23%), Postives = 567/583 (97.26%), Query Frame = 0

Query: 17  MRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 76
           M+SS+AFRFTTFI FLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE
Sbjct: 1   MQSSHAFRFTTFILFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 60

Query: 77  VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVD 136
           VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVD
Sbjct: 61  VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVD 120

Query: 137 GEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH 196
           GEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH
Sbjct: 121 GEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH 180

Query: 197 DYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT 256
           DYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT
Sbjct: 181 DYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT 240

Query: 257 YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF--- 316
           YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYR+DTRVSSNTSGNGLYDFIPF   
Sbjct: 241 YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRRDTRVSSNTSGNGLYDFIPFRPT 300

Query: 317 -----------QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR 376
                      +EYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR
Sbjct: 301 PSPALAIVRASEEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR 360

Query: 377 LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 436
           LQSSGSCLDSVNDLRITACPW+PSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF
Sbjct: 361 LQSSGSCLDSVNDLRITACPWDPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 420

Query: 437 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 496
           CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK
Sbjct: 421 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 480

Query: 497 YGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDK 556
           YGGSPHWGKNRNIAFLKA+GKYKNREGFLKIKNEYDP GLFSNEWTNQILGVEGTVSIDK
Sbjct: 481 YGGSPHWGKNRNIAFLKAVGKYKNREGFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDK 540

Query: 557 DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFK 586
           DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFK
Sbjct: 541 DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFK 583

BLAST of Carg24187 vs. NCBI nr
Match: XP_022943972.1 (probable L-gulonolactone oxidase 6 [Cucurbita moschata])

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 558/583 (95.71%), Postives = 563/583 (96.57%), Query Frame = 0

Query: 17  MRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 76
           M+SSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE
Sbjct: 1   MQSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 60

Query: 77  VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVD 136
           VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACP GVDGVVISTKYLNRVLKVD
Sbjct: 61  VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPHGVDGVVISTKYLNRVLKVD 120

Query: 137 GEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH 196
           GEGM ITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH
Sbjct: 121 GEGMRITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH 180

Query: 197 DYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT 256
           DYVVGIKMVSPGGAQDGYAKVRRLVDG QHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT
Sbjct: 181 DYVVGIKMVSPGGAQDGYAKVRRLVDGHQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT 240

Query: 257 YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF--- 316
           YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKV+YR+DTRVSSNTSGNGLYDFIPF   
Sbjct: 241 YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVMYRRDTRVSSNTSGNGLYDFIPFRPT 300

Query: 317 -----------QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR 376
                      +EYQEARRDAAGKC SAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR
Sbjct: 301 PSAALAIVRASEEYQEARRDAAGKCRSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR 360

Query: 377 LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 436
           LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF
Sbjct: 361 LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 420

Query: 437 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 496
           CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK
Sbjct: 421 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 480

Query: 497 YGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDK 556
           YGGSPHWGKNRNIAFL+AIGKYKNRE FLKIKNEYDP GLFSNEWTNQILGVEGTVSIDK
Sbjct: 481 YGGSPHWGKNRNIAFLEAIGKYKNRERFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDK 540

Query: 557 DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFK 586
           DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFK
Sbjct: 541 DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFK 583

BLAST of Carg24187 vs. NCBI nr
Match: XP_022975260.1 (LOW QUALITY PROTEIN: probable L-gulonolactone oxidase 6 [Cucurbita maxima])

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 549/583 (94.17%), Postives = 559/583 (95.88%), Query Frame = 0

Query: 17  MRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 76
           MRSS+AFRFTT I FLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE
Sbjct: 1   MRSSHAFRFTTLILFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 60

Query: 77  VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVD 136
           VKYPTTEAE+ISAVA ATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTK+LN VLKVD
Sbjct: 61  VKYPTTEAEMISAVAAATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKFLNWVLKVD 120

Query: 137 GEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH 196
           GEGMSITVEGGVTVREIIEVAAA GLALPHAPYW GLTIGGLLSTGAHGSSLWGKGSAVH
Sbjct: 121 GEGMSITVEGGVTVREIIEVAAAVGLALPHAPYWXGLTIGGLLSTGAHGSSLWGKGSAVH 180

Query: 197 DYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT 256
           DYVVG+KMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQ TLKLQPMFKRSIT
Sbjct: 181 DYVVGMKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQFTLKLQPMFKRSIT 240

Query: 257 YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF--- 316
           YFERDDTDLSD+VISFGRSYEFADMTWYPSQRKVVYR DTRVSSNTSGNGLYDFIPF   
Sbjct: 241 YFERDDTDLSDEVISFGRSYEFADMTWYPSQRKVVYRTDTRVSSNTSGNGLYDFIPFCPT 300

Query: 317 -----------QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR 376
                      +EYQEARRDAAGKCLSAKIITSLLSLSAYGLTN+GITFLKYPVIGYNNR
Sbjct: 301 PSAGLAIVRASKEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNDGITFLKYPVIGYNNR 360

Query: 377 LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 436
           LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF
Sbjct: 361 LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 420

Query: 437 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 496
           CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK
Sbjct: 421 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 480

Query: 497 YGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDK 556
           YGG PHWGKNRNIAFL+AIGKY+NREGFLKIKNEYDP GLFSNEWTNQILGVEGTVSIDK
Sbjct: 481 YGGIPHWGKNRNIAFLEAIGKYRNREGFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDK 540

Query: 557 DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFK 586
           DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSY+GFK
Sbjct: 541 DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYIGFK 583

BLAST of Carg24187 vs. NCBI nr
Match: KAG6597012.1 (putative L-gulonolactone oxidase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 542/560 (96.79%), Postives = 545/560 (97.32%), Query Frame = 0

Query: 17  MRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 76
           MRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE
Sbjct: 1   MRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 60

Query: 77  VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVD 136
           VKYPTTE EVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVD
Sbjct: 61  VKYPTTEGEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVD 120

Query: 137 GEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH 196
           GEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH
Sbjct: 121 GEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH 180

Query: 197 DYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT 256
           DYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT
Sbjct: 181 DYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT 240

Query: 257 YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF--- 316
           YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYR+DTRVSSNTSGNGLYDFIPF   
Sbjct: 241 YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRRDTRVSSNTSGNGLYDFIPFRPT 300

Query: 317 -----------QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR 376
                      +EYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR
Sbjct: 301 PSAALAIVRASEEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR 360

Query: 377 LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 436
           LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF
Sbjct: 361 LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 420

Query: 437 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 496
           CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK
Sbjct: 421 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 480

Query: 497 YGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDK 556
           YGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDK
Sbjct: 481 YGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDK 540

Query: 557 DGCALEGLCLCSKDSHCAPS 563
           DGCALEGLCLCSKDSHCAP+
Sbjct: 541 DGCALEGLCLCSKDSHCAPN 560

BLAST of Carg24187 vs. ExPASy Swiss-Prot
Match: O81032 (Probable L-gulonolactone oxidase 6 OS=Arabidopsis thaliana OX=3702 GN=GULLO6 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 2.8e-212
Identity = 354/576 (61.46%), Postives = 434/576 (75.35%), Query Frame = 0

Query: 22  AFRFTTFIFFLV-LLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYP 81
           AF  T F+   +  LV  + STPPEDP+KC S N NCT+TN+Y  FPDR+ CR   V YP
Sbjct: 15  AFWRTIFVVHCISTLVFTTISTPPEDPVKCVSGNTNCTVTNSYGAFPDRSTCRAANVAYP 74

Query: 82  TTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGM 141
           TTEAE+IS VA AT+A RK++V TR+ HSI KLAC DG DG++ISTK+LN  ++ D   M
Sbjct: 75  TTEAELISVVAAATKAGRKMRVTTRYSHSITKLACTDGTDGLLISTKFLNHTVRTDATAM 134

Query: 142 SITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVV 201
           ++TVE GVT+R++I  AA  GLALP+APYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV 
Sbjct: 135 TLTVESGVTLRQLIAEAAKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVT 194

Query: 202 GIKMVSPGGAQDGYAKVR--RLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYF 261
            I++VSPG   DG+AKVR  R     +  NAAKVSLGVLG+I+QVTLKLQPMFKRS+ Y 
Sbjct: 195 EIRIVSPGSVNDGFAKVRVLRETTTPKEFNAAKVSLGVLGVISQVTLKLQPMFKRSLRYV 254

Query: 262 ERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF----- 321
            R+D+D  DQ ++FG  +EFAD  W PSQ KVVYR D RV+ NTSGNGL DF+PF     
Sbjct: 255 MRNDSDFGDQAVTFGMKHEFADFIWLPSQGKVVYRMDDRVAVNTSGNGLLDFMPFRSQLS 314

Query: 322 ---------QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQ 381
                    +E QE  RDA GKC  A +ITS L  ++YGLTNNG+ F  YPVIG  NR+ 
Sbjct: 315 AALAIIRSSEETQERFRDANGKCAGATLITSTLFATSYGLTNNGMIFTGYPVIGSQNRMM 374

Query: 382 SSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCG 441
           SSGSCLDS++D  ITACPW+  IK EFFH+   SI L  VK FI D++ L ++E  + C 
Sbjct: 375 SSGSCLDSLHDGLITACPWDSRIKSEFFHQTTFSIPLTQVKSFINDIKSLVKIESKSLCV 434

Query: 442 LELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYG 501
           LELY+GILMRYV +S AYLGKE + ++FD TYYR+KDP+SPRL+ED +EE+EQ+ALFKY 
Sbjct: 435 LELYDGILMRYVTSSPAYLGKETEALDFDLTYYRAKDPLSPRLYEDFIEEIEQIALFKYN 494

Query: 502 GSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDG 561
             PHWGKNRN+AF   I KYKN   FLK+K  YDP GLFS+EWT+QILG++G V+I KDG
Sbjct: 495 ALPHWGKNRNLAFDGVIKKYKNVPAFLKVKESYDPMGLFSSEWTDQILGIKGNVTIIKDG 554

Query: 562 CALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCS 581
           CALEGLC+CS+D+HCAP+KGYFCR G++YKEARVC+
Sbjct: 555 CALEGLCVCSEDAHCAPTKGYFCRPGKVYKEARVCT 590

BLAST of Carg24187 vs. ExPASy Swiss-Prot
Match: Q6NQ66 (L-gulonolactone oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GULLO2 PE=1 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 2.2e-209
Identity = 339/575 (58.96%), Postives = 433/575 (75.30%), Query Frame = 0

Query: 22  AFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYPT 81
           ++R    ++++  L+  + STPP+DP+KC S N NCT+TN+Y  FPDR+ CR   V YP 
Sbjct: 8   SYRTLVGLYYIFTLMHTAVSTPPDDPVKCVSGNTNCTVTNSYGAFPDRSTCRAANVAYPK 67

Query: 82  TEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMS 141
           TEAE++S VA AT+A RK++V TR+ HSI KL C DG +G+ ISTK+LN  ++ D   M+
Sbjct: 68  TEAELVSVVAAATQAGRKMRVTTRYSHSITKLVCTDGTEGLFISTKFLNHTVQADATAMT 127

Query: 142 ITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVG 201
           +TVE G+T+R++I  AA  GLALP+APYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV  
Sbjct: 128 MTVESGMTLRQLIVEAAKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTE 187

Query: 202 IKMVSPGGAQDGYAKVRRLVD--GDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFE 261
           I+MVSPG   +G+AK+R L +       NAAKVSLGVLG+I+QVT +LQPMFKRS+TY  
Sbjct: 188 IRMVSPGSVNEGFAKIRILSETTTPNEFNAAKVSLGVLGVISQVTFELQPMFKRSLTYTM 247

Query: 262 RDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF------ 321
           R+D+D  DQ ++FG+ +EFAD  W PSQ KVVYR+D RV+ NTSGNGL+DF+PF      
Sbjct: 248 RNDSDFEDQAVTFGKKHEFADFIWLPSQGKVVYRRDDRVAVNTSGNGLFDFLPFRSQLSA 307

Query: 322 --------QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQS 381
                   +E QE  RDA GKC+ A II+S L   +YGLTNNGI F  YPV+G  NR+ S
Sbjct: 308 AIAIIRTSEETQERFRDANGKCVGATIISSTLFAPSYGLTNNGIIFTGYPVVGSQNRMMS 367

Query: 382 SGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGL 441
           SGSCLDS+ D  ITAC W+  IKGEFFH+  +S+ L  VK FI D++ L ++E  + CGL
Sbjct: 368 SGSCLDSLQDGLITACAWDSRIKGEFFHQTTLSVPLTQVKSFISDIKSLVKIEQKSLCGL 427

Query: 442 ELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYGG 501
           EL+ GILMRYV +S AYLGKE + ++FD TYYR+KDP++PRL+ED +EE+EQ+ALFKY  
Sbjct: 428 ELHYGILMRYVTSSPAYLGKETEALDFDITYYRAKDPLTPRLYEDFIEEIEQIALFKYNA 487

Query: 502 SPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDGC 561
            PHWGKNRN+AF   I KY N   FLK+K+ YDP GLFS+EWT+QILG++G  SI KDGC
Sbjct: 488 LPHWGKNRNLAFDGVIRKYNNAPAFLKVKDSYDPKGLFSSEWTDQILGIKGNASIVKDGC 547

Query: 562 ALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCS 581
           ALEGLC+CSKD+HCAP+KGY CR G++YKEARVC+
Sbjct: 548 ALEGLCICSKDAHCAPAKGYLCRPGKVYKEARVCT 582

BLAST of Carg24187 vs. ExPASy Swiss-Prot
Match: O81030 (L-gulonolactone oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=GULLO5 PE=1 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 2.5e-205
Identity = 336/570 (58.95%), Postives = 422/570 (74.04%), Query Frame = 0

Query: 29  IFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYPTTEAEVIS 88
           ++ L  LV    STPPEDP+KC S N NC +TN+   FPDR+ CR   V YPTTEAE++S
Sbjct: 19  LYCLFTLVHTVISTPPEDPVKCVSGNTNCIVTNSLGAFPDRSTCRAANVAYPTTEAELVS 78

Query: 89  AVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVEGGV 148
            VA AT+A RK++V TR+ HSIPKL C DG DG+ ISTK+LN  ++ D + M++TVE GV
Sbjct: 79  IVAAATKAGRKMRVTTRYSHSIPKLTCTDGNDGLFISTKFLNHTVQADAKAMTLTVESGV 138

Query: 149 TVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVSPG 208
           T+R++I  AA  GLALP+APYWWG+T+GG++ TGAHGSSLWGKGSAVHDYV  I+MVSPG
Sbjct: 139 TLRQLIAEAAKVGLALPYAPYWWGVTVGGMMGTGAHGSSLWGKGSAVHDYVTEIRMVSPG 198

Query: 209 GAQDGYAKVRRLVD--GDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDTDLS 268
              DG+AK+R L +       NAAKVSLGVLG+I+QVT +LQPMFKRS+ Y  R+D D +
Sbjct: 199 SVNDGFAKIRVLSETTTPNEFNAAKVSLGVLGVISQVTFELQPMFKRSLKYVMRNDLDFN 258

Query: 269 DQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF------------- 328
           D+ ++FG+ +EFAD  W PSQ KVVYR D RV+ NT GNGLYDF PF             
Sbjct: 259 DEALTFGKKHEFADFVWLPSQGKVVYRMDDRVAVNTLGNGLYDFFPFRSQLSAVLATTRS 318

Query: 329 -QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDS 388
            +E QE  RDA GKC++A  I+S L  ++YGLTNNGITF  YPVIG  NR+ SSGSCLD 
Sbjct: 319 SEETQETLRDAHGKCVTATTISSTLFSTSYGLTNNGITFTGYPVIGSQNRMMSSGSCLDG 378

Query: 389 VNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYNGIL 448
           + D   +AC W+  +KG F+H+   SI L  VK FI D++ L +++  + CGLELY GIL
Sbjct: 379 LEDKLTSACAWDSRVKGVFYHQTTFSIPLTQVKSFINDIKSLLKIDSKSLCGLELYYGIL 438

Query: 449 MRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYGGSPHWGKN 508
           MRYV +S AYLGKE + I+FD TYYR+ DP++PRL+ED +EE+EQ+AL KY   PHWGKN
Sbjct: 439 MRYVTSSPAYLGKETEAIDFDITYYRANDPLTPRLYEDFIEEIEQIALLKYNALPHWGKN 498

Query: 509 RNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDGCALEGLCL 568
           RN+AF   I KYKN   FLK+K  YDP GLFS+EWT+QILG++G  +I KDGCALEGLC+
Sbjct: 499 RNLAFDGVIKKYKNAPAFLKVKESYDPNGLFSSEWTDQILGIKGNPTIVKDGCALEGLCI 558

Query: 569 CSKDSHCAPSKGYFCRAGRIYKEARVCSYL 583
           CS D+HCAPSKGY CR G++YKEARVC+++
Sbjct: 559 CSDDAHCAPSKGYLCRPGKVYKEARVCTHV 588

BLAST of Carg24187 vs. ExPASy Swiss-Prot
Match: Q9C614 (Probable L-gulonolactone oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GULLO1 PE=3 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 4.5e-202
Identity = 339/574 (59.06%), Postives = 425/574 (74.04%), Query Frame = 0

Query: 27  TFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYPTTEAEV 86
           + IFF     + + STPP+DP+KC S N  CT+TN+Y  FPDR+IC   +V+YP TEAE+
Sbjct: 6   SLIFF--CFCTFASSTPPDDPVKCESGNNMCTVTNSYGAFPDRSICEAAKVEYPKTEAEL 65

Query: 87  ISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVEG 146
           +S VA ATRA +K++V TR+ HSIPKL C DG DGV+ISTK+LN V+  + E  ++TVE 
Sbjct: 66  VSIVAAATRAGQKVRVVTRYVHSIPKLVCTDGKDGVLISTKFLNNVVGTNPEAKTLTVES 125

Query: 147 GVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVS 206
           GVT+R++I  AA   LALPHAPYWWGLT+GGL+ TGAHGSSLWGKGSAVHDYV  I+MVS
Sbjct: 126 GVTLRQLIGEAAELELALPHAPYWWGLTVGGLMGTGAHGSSLWGKGSAVHDYVSEIRMVS 185

Query: 207 PGGAQDGYAKVRRL---VDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDT 266
           PG A DGY KVR L   +D D+   AAKVSLGVLG+I+QVT +LQPMFKRS+T+  ++D+
Sbjct: 186 PGLASDGYVKVRVLSETIDPDE-FRAAKVSLGVLGVISQVTFQLQPMFKRSLTFVMQNDS 245

Query: 267 DLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF---------- 326
           D  DQ ++FG  +EFAD  W PSQ KVVYR D RV  NTSGNGL+DF PF          
Sbjct: 246 DFGDQAVTFGEKHEFADFLWLPSQGKVVYRMDDRVPVNTSGNGLFDFFPFRPQLSVALAI 305

Query: 327 ----QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSC 386
               +E +E+  DA  KC  A+ ITS L   +YG+TNNG+ F  YPVIG  N + SSG+C
Sbjct: 306 IRSLEESEESSGDANDKCARAEQITSFLFSISYGVTNNGMEFTGYPVIGKQNHMMSSGTC 365

Query: 387 LDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYN 446
           LDS  D  IT+CPW+P IKG+FFH+   SI L  VKGFI D++ L ++EP + C LE  N
Sbjct: 366 LDSHQDGLITSCPWDPRIKGQFFHQTAFSIPLTRVKGFINDIKALVKIEPKSLCALERSN 425

Query: 447 GILMRYVKASTAYLGKEEDGINFDFTYYRSK-DPMSPRLFEDVLEEVEQMALFKYGGSPH 506
           GIL+RYV +S A+LGKEE  ++FD TYYRSK DP++PRL+ED +EE+EQMA+FKY   PH
Sbjct: 426 GILIRYVTSSPAFLGKEEKALDFDLTYYRSKDDPLAPRLYEDFIEEIEQMAIFKYNALPH 485

Query: 507 WGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDGCALE 566
           WGKNRN+AF   I KYKN   FLK+K  +DP GLFS EWTNQILG++G V+I K+GCALE
Sbjct: 486 WGKNRNLAFDGVIRKYKNANTFLKVKERFDPLGLFSTEWTNQILGLKGNVTIVKEGCALE 545

Query: 567 GLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYL 583
           GLC+CS D+HCAP KGY CR G++Y +ARVC+++
Sbjct: 546 GLCVCSDDAHCAPKKGYLCRPGKVYTKARVCTHV 576

BLAST of Carg24187 vs. ExPASy Swiss-Prot
Match: Q9FM82 (Probable L-gulonolactone oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GULLO4 PE=2 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 3.5e-199
Identity = 329/570 (57.72%), Postives = 422/570 (74.04%), Query Frame = 0

Query: 28  FIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYPTTEAEVI 87
           F FF     + + S+PP+DP+ C   N  CT+TN+Y  FPDR+IC+  +V+YP TEAE++
Sbjct: 9   FCFF-----TFASSSPPDDPVNCEFGNTMCTVTNSYGAFPDRSICKAAKVEYPRTEAELV 68

Query: 88  SAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVEGG 147
           S VA ATRA +K++V TR+ HS PKL C DG DG +ISTK+LN V+  + E  ++TVE G
Sbjct: 69  SIVAAATRAGQKMRVVTRYSHSFPKLVCTDGKDGTLISTKFLNHVVTTNPEAKTLTVESG 128

Query: 148 VTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVSP 207
           VT+R++IE AA   LALP+APYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV  I++VSP
Sbjct: 129 VTLRQLIEEAAKFDLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTEIRLVSP 188

Query: 208 GGAQDGYAKVRRLVD--GDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDTDL 267
           G A DGY KV+ L +    +   AAKVSLGVLG+I+QVT +LQPMFKRS+ Y  R+D+D 
Sbjct: 189 GLASDGYVKVQVLSETIDPEEFRAAKVSLGVLGVISQVTFELQPMFKRSLNYVMRNDSDF 248

Query: 268 SDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF------------ 327
            DQ +SFG  +EFAD  W PSQ KVVYR D RV  NTSG+GL++F PF            
Sbjct: 249 GDQAVSFGERHEFADFLWLPSQGKVVYRMDGRVPLNTSGDGLFEFFPFRSQLSLVLAIDR 308

Query: 328 --QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSCLD 387
             +E +E+  DA  KC+ AK+++S + L +YG+TNNG+ F  YPVIG  N + SSGSCLD
Sbjct: 309 SLEESEESLEDANMKCVRAKLVSSSMFLMSYGVTNNGLIFTGYPVIGMQNHMMSSGSCLD 368

Query: 388 SVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYNGI 447
           S  D  ITACPW+P IKG+FFH+   S+ L  VK FI D++ L ++EP + C LE  NGI
Sbjct: 369 SRQDGLITACPWDPRIKGQFFHQTTFSVSLTNVKSFINDIKALVKIEPKSLCVLEGSNGI 428

Query: 448 LMRYVKASTAYLGKEEDGINFDFTYYRSK-DPMSPRLFEDVLEEVEQMALFKYGGSPHWG 507
           L+RYV +S A+LGKEE  ++FD TYYRSK DP++PRL+ED +EE+EQMA+FKY   PHWG
Sbjct: 429 LIRYVTSSLAFLGKEEKALDFDLTYYRSKNDPLAPRLYEDYIEEIEQMAIFKYNALPHWG 488

Query: 508 KNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDGCALEGL 567
           KNRN+AF  AI KYKN   FLK+K ++D  GLFS EWT+QILG++G V+I K GCALEGL
Sbjct: 489 KNRNLAFDGAIRKYKNANAFLKVKEKFDSLGLFSTEWTDQILGLKGNVTIVKQGCALEGL 548

Query: 568 CLCSKDSHCAPSKGYFCRAGRIYKEARVCS 581
           C+CS+DSHCAP+KGY CR G++Y+EARVC+
Sbjct: 549 CICSEDSHCAPTKGYLCRPGKVYREARVCT 573

BLAST of Carg24187 vs. ExPASy TrEMBL
Match: A0A6J1FUH8 (L-gulonolactone oxidase OS=Cucurbita moschata OX=3662 GN=LOC111448536 PE=3 SV=1)

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 558/583 (95.71%), Postives = 563/583 (96.57%), Query Frame = 0

Query: 17  MRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 76
           M+SSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE
Sbjct: 1   MQSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 60

Query: 77  VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVD 136
           VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACP GVDGVVISTKYLNRVLKVD
Sbjct: 61  VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPHGVDGVVISTKYLNRVLKVD 120

Query: 137 GEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH 196
           GEGM ITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH
Sbjct: 121 GEGMRITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH 180

Query: 197 DYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT 256
           DYVVGIKMVSPGGAQDGYAKVRRLVDG QHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT
Sbjct: 181 DYVVGIKMVSPGGAQDGYAKVRRLVDGHQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT 240

Query: 257 YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF--- 316
           YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKV+YR+DTRVSSNTSGNGLYDFIPF   
Sbjct: 241 YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVMYRRDTRVSSNTSGNGLYDFIPFRPT 300

Query: 317 -----------QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR 376
                      +EYQEARRDAAGKC SAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR
Sbjct: 301 PSAALAIVRASEEYQEARRDAAGKCRSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR 360

Query: 377 LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 436
           LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF
Sbjct: 361 LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 420

Query: 437 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 496
           CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK
Sbjct: 421 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 480

Query: 497 YGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDK 556
           YGGSPHWGKNRNIAFL+AIGKYKNRE FLKIKNEYDP GLFSNEWTNQILGVEGTVSIDK
Sbjct: 481 YGGSPHWGKNRNIAFLEAIGKYKNRERFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDK 540

Query: 557 DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFK 586
           DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFK
Sbjct: 541 DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFK 583

BLAST of Carg24187 vs. ExPASy TrEMBL
Match: A0A6J1IG87 (L-gulonolactone oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474382 PE=3 SV=1)

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 549/583 (94.17%), Postives = 559/583 (95.88%), Query Frame = 0

Query: 17  MRSSNAFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 76
           MRSS+AFRFTT I FLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE
Sbjct: 1   MRSSHAFRFTTLILFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGE 60

Query: 77  VKYPTTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVD 136
           VKYPTTEAE+ISAVA ATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTK+LN VLKVD
Sbjct: 61  VKYPTTEAEMISAVAAATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKFLNWVLKVD 120

Query: 137 GEGMSITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVH 196
           GEGMSITVEGGVTVREIIEVAAA GLALPHAPYW GLTIGGLLSTGAHGSSLWGKGSAVH
Sbjct: 121 GEGMSITVEGGVTVREIIEVAAAVGLALPHAPYWXGLTIGGLLSTGAHGSSLWGKGSAVH 180

Query: 197 DYVVGIKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSIT 256
           DYVVG+KMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQ TLKLQPMFKRSIT
Sbjct: 181 DYVVGMKMVSPGGAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQFTLKLQPMFKRSIT 240

Query: 257 YFERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF--- 316
           YFERDDTDLSD+VISFGRSYEFADMTWYPSQRKVVYR DTRVSSNTSGNGLYDFIPF   
Sbjct: 241 YFERDDTDLSDEVISFGRSYEFADMTWYPSQRKVVYRTDTRVSSNTSGNGLYDFIPFCPT 300

Query: 317 -----------QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNR 376
                      +EYQEARRDAAGKCLSAKIITSLLSLSAYGLTN+GITFLKYPVIGYNNR
Sbjct: 301 PSAGLAIVRASKEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNDGITFLKYPVIGYNNR 360

Query: 377 LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 436
           LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF
Sbjct: 361 LQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAF 420

Query: 437 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 496
           CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK
Sbjct: 421 CGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFK 480

Query: 497 YGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDK 556
           YGG PHWGKNRNIAFL+AIGKY+NREGFLKIKNEYDP GLFSNEWTNQILGVEGTVSIDK
Sbjct: 481 YGGIPHWGKNRNIAFLEAIGKYRNREGFLKIKNEYDPYGLFSNEWTNQILGVEGTVSIDK 540

Query: 557 DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYLGFK 586
           DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSY+GFK
Sbjct: 541 DGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYIGFK 583

BLAST of Carg24187 vs. ExPASy TrEMBL
Match: A0A1S3AUG3 (L-gulonolactone oxidase OS=Cucumis melo OX=3656 GN=LOC103483108 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 2.2e-260
Identity = 438/569 (76.98%), Postives = 491/569 (86.29%), Query Frame = 0

Query: 33  VLLVSVSESTPPEDPIKCR--SPN--ANCTITNAYATFPDRAICRVGEVKYPTTEAEVIS 92
           + LV VSE +PPEDPIKC   SPN  ANCTITN+Y  FPDR+ICRV EV YPTTEAEV+ 
Sbjct: 22  MFLVLVSECSPPEDPIKCGSVSPNKYANCTITNSYGAFPDRSICRVAEVIYPTTEAEVML 81

Query: 93  AVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVEGGV 152
           AVA ATR +RK+KVGTRF HSIPKLACP+G +GVVISTKYLNR +KVD EGMSI+VEGG 
Sbjct: 82  AVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISVEGGA 141

Query: 153 TVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVSPG 212
           T+R+IIE AA  GLALPH PYWWGLTIGGLLSTGAHGSSLWGKGS+VHDYVV +KMVSPG
Sbjct: 142 TLRQIIEDAATAGLALPHTPYWWGLTIGGLLSTGAHGSSLWGKGSSVHDYVVAVKMVSPG 201

Query: 213 GAQDGYAKVRRLVDGDQ-HLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDTDLSD 272
           G++DGYAK+R ++  DQ HLNAAKVSLGVLG+ITQVTLKLQPMFKRSITY +RDD DL D
Sbjct: 202 GSEDGYAKLRTILPDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDDKDLGD 261

Query: 273 QVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF-------------- 332
           +V+ FG  YEF DMTWYPS RK+VYR DTRV SNTSGNG+YDFIPF              
Sbjct: 262 EVLRFGTHYEFGDMTWYPSHRKIVYRIDTRVPSNTSGNGVYDFIPFRPTPSIGLALIRAS 321

Query: 333 QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSV 392
           +E QE+RRDAA KC S  +ITS LSL AYGLTNNGITFLKYPVIGYNNRLQSSGSCLDS+
Sbjct: 322 EEEQESRRDAAAKCSSGNLITSTLSLLAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSL 381

Query: 393 NDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYNGILM 452
           NDLRIT CPW+PSIKGEFFH+  ISI+L +VK FIEDV++L ELEPMAFCGLELYNGILM
Sbjct: 382 NDLRITTCPWDPSIKGEFFHQTAISIELTMVKSFIEDVQRLAELEPMAFCGLELYNGILM 441

Query: 453 RYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYGGSPHWGKNR 512
           RYV+ASTAYLGK++DG++FDFTYYRS+DPMSPRLFEDV+EEVEQMA+FKYGG PHWGKNR
Sbjct: 442 RYVRASTAYLGKQQDGVDFDFTYYRSRDPMSPRLFEDVIEEVEQMAVFKYGGIPHWGKNR 501

Query: 513 NIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDGCALEGLCLC 572
           N+AF +   KY++RE FLKI  EYDP GLFSNEWT+QILGVEGTV I KDGCALEGLC+C
Sbjct: 502 NVAFHQVFHKYRDREKFLKIMKEYDPHGLFSNEWTDQILGVEGTVIIYKDGCALEGLCIC 561

Query: 573 SKDSHCAPSKGYFCRAGRIYKEARVCSYL 583
           S+ +HCAPSKGYFCRAGRIYK+ARVCSYL
Sbjct: 562 SQHNHCAPSKGYFCRAGRIYKDARVCSYL 590

BLAST of Carg24187 vs. ExPASy TrEMBL
Match: A0A0A0L3K4 (L-gulonolactone oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G116800 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.4e-254
Identity = 440/586 (75.09%), Postives = 486/586 (82.94%), Query Frame = 0

Query: 33  VLLVSVSESTPPEDPIKC--RSPN--ANCTITNAYATFPDRAICRVGEVKYPTTEAEVIS 92
           + LV VSE +PP DPIKC   SPN  ANCTITN Y +FPDR+ICRV EV YPTTEAEV+ 
Sbjct: 21  MFLVLVSECSPPGDPIKCVSVSPNKYANCTITNFYGSFPDRSICRVAEVIYPTTEAEVML 80

Query: 93  AVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVEGGV 152
           AVA ATR +RK+KVGTRF HSIPKLACP+G +GVVISTKYLNR +KVD EGMSI+VEGG 
Sbjct: 81  AVAAATRGLRKMKVGTRFSHSIPKLACPEGEEGVVISTKYLNRTVKVDVEGMSISVEGGA 140

Query: 153 TVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVSPG 212
           T+R+IIE AA  GLALP  PYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVV ++MVSPG
Sbjct: 141 TLRQIIEDAATAGLALPSTPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVAVQMVSPG 200

Query: 213 GAQDGYAKVRRLVDGDQ-HLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDTDLSD 272
           G +DGYAK R ++  DQ HLNAAKVSLGVLG+ITQVTLKLQPMFKRSITY +RDD DL D
Sbjct: 201 GPEDGYAKQRTILPDDQNHLNAAKVSLGVLGVITQVTLKLQPMFKRSITYLKRDDKDLGD 260

Query: 273 QVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF-------------- 332
           +VI FG+SYEF DM+WYPSQRKVVYR DTRV SNTSGNGLYDFIPF              
Sbjct: 261 EVIRFGKSYEFGDMSWYPSQRKVVYRIDTRVPSNTSGNGLYDFIPFRPTLSVELALLRAS 320

Query: 333 QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNN-----------------GITFLKYPV 392
           +E QE+RRDAA KCLS  +ITS LSL AYGLTNN                 GITFLKYPV
Sbjct: 321 EEEQESRRDAAAKCLSGNLITSTLSLLAYGLTNNVILNKMYNIYMSYINIVGITFLKYPV 380

Query: 393 IGYNNRLQSSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGE 452
           IGYNNRLQSSGSCLDS+ND RITACPW+PSIKGEFF+E  +SI+L VVK FIEDV+KL E
Sbjct: 381 IGYNNRLQSSGSCLDSLNDFRITACPWDPSIKGEFFYETSVSIELTVVKSFIEDVQKLAE 440

Query: 453 LEPMAFCGLELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVE 512
           LEPMAFCGLELYNGILMRYV ASTAYLGK++D + FD TYYRSKDPMSPRLFEDV+EEVE
Sbjct: 441 LEPMAFCGLELYNGILMRYVTASTAYLGKQQDSVEFDLTYYRSKDPMSPRLFEDVIEEVE 500

Query: 513 QMALFKYGGSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEG 572
           QMA+FKYGG PHWGKNRN+AF +   KY+N E FLKI  EYDP  LFSNEWT+QILGVEG
Sbjct: 501 QMAVFKYGGIPHWGKNRNVAFHQVFHKYRNIEKFLKIMKEYDPHRLFSNEWTDQILGVEG 560

Query: 573 TVSIDKDGCALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYL 583
           TV+I KDGCALEGLC+CSK +HCAPSKGYFCRAG+IYK+ARVCSYL
Sbjct: 561 TVNIYKDGCALEGLCICSKHTHCAPSKGYFCRAGKIYKDARVCSYL 606

BLAST of Carg24187 vs. ExPASy TrEMBL
Match: A0A2P5A4Y9 (L-gulonolactone oxidase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_368830 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 1.1e-227
Identity = 374/566 (66.08%), Postives = 457/566 (80.74%), Query Frame = 0

Query: 29  IFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYPTTEAEVIS 88
           ++ L LL+ V   TPPEDPIKC + N+NCT+TN+Y  FPDR+ICR G   YP+TE E+I+
Sbjct: 18  LYCLFLLLLVVCCTPPEDPIKCSTGNSNCTVTNSYGAFPDRSICRAGAATYPSTEEELIA 77

Query: 89  AVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVEGGV 148
            VA ATR   K+KV TRF HSIPKL CP   DG++ISTK+LNRVL +D E M++TVE GV
Sbjct: 78  IVASATRDGSKMKVATRFSHSIPKLVCPGQDDGLLISTKHLNRVLNIDTEAMTMTVESGV 137

Query: 149 TVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVSPG 208
           T+R++I  AA  GLALP+ PYWWGLTIGGLLSTGAHGS+LWGKGS+VHDYV+G+ +VSPG
Sbjct: 138 TLRDLISEAAKAGLALPYTPYWWGLTIGGLLSTGAHGSTLWGKGSSVHDYVIGLTIVSPG 197

Query: 209 GAQDGYAKVRRLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDTDLSDQ 268
           GA DG+ KVRRL DGD+ LNAAK SLGVLG+I+QVTLKLQP+FKRSITY  ++D+DL D+
Sbjct: 198 GAADGFVKVRRLEDGDEELNAAKASLGVLGVISQVTLKLQPLFKRSITYVTKNDSDLGDE 257

Query: 269 VISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF--------------Q 328
           VISFGR +EFAD+TWYPSQ+K VYR D RVS+N SG+GLYDFIPF              +
Sbjct: 258 VISFGRKHEFADITWYPSQQKAVYRVDDRVSTNNSGDGLYDFIPFRATTSLALALIRTTE 317

Query: 329 EYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDSVN 388
           + QE+  DA GKC+ AK++T+ LS SAYGLTN+GI F+ YPVIGY +RLQ+SG+CLDS+ 
Sbjct: 318 DNQESLDDADGKCIGAKLLTTTLSTSAYGLTNDGILFMGYPVIGYQHRLQASGTCLDSLQ 377

Query: 389 DLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYNGILMR 448
           D RITAC W+  +KGEFFH+   S+ L  VK FI+DV+ L +LEP A CG+ELYNGILMR
Sbjct: 378 DARITACSWDSRVKGEFFHQSTFSVPLSSVKSFIQDVQNLVKLEPKALCGVELYNGILMR 437

Query: 449 YVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYGGSPHWGKNRN 508
           YV AS+AYLGK+ D I+FD TYYRSKDP+SPRL+EDVLEE+EQMA FKYG  PHWGKNRN
Sbjct: 438 YVTASSAYLGKQWDAIDFDITYYRSKDPLSPRLYEDVLEEIEQMAAFKYGALPHWGKNRN 497

Query: 509 IAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDGCALEGLCLCS 568
           +AF  A  KYKN + FL++K  YDP GLFS+EWTNQ+LG++G ++I K+GCALEGLC C+
Sbjct: 498 VAFEGAFEKYKNGQEFLRVKKFYDPLGLFSSEWTNQVLGLKGGLTIVKEGCALEGLCRCT 557

Query: 569 KDSHCAPSKGYFCRAGRIYKEARVCS 581
           +DSHCAPSKGYFCR G+IYK+ARVCS
Sbjct: 558 EDSHCAPSKGYFCRPGKIYKDARVCS 583

BLAST of Carg24187 vs. TAIR 10
Match: AT2G46760.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 739.6 bits (1908), Expect = 2.0e-213
Identity = 354/576 (61.46%), Postives = 434/576 (75.35%), Query Frame = 0

Query: 22  AFRFTTFIFFLV-LLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYP 81
           AF  T F+   +  LV  + STPPEDP+KC S N NCT+TN+Y  FPDR+ CR   V YP
Sbjct: 15  AFWRTIFVVHCISTLVFTTISTPPEDPVKCVSGNTNCTVTNSYGAFPDRSTCRAANVAYP 74

Query: 82  TTEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGM 141
           TTEAE+IS VA AT+A RK++V TR+ HSI KLAC DG DG++ISTK+LN  ++ D   M
Sbjct: 75  TTEAELISVVAAATKAGRKMRVTTRYSHSITKLACTDGTDGLLISTKFLNHTVRTDATAM 134

Query: 142 SITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVV 201
           ++TVE GVT+R++I  AA  GLALP+APYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV 
Sbjct: 135 TLTVESGVTLRQLIAEAAKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVT 194

Query: 202 GIKMVSPGGAQDGYAKVR--RLVDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYF 261
            I++VSPG   DG+AKVR  R     +  NAAKVSLGVLG+I+QVTLKLQPMFKRS+ Y 
Sbjct: 195 EIRIVSPGSVNDGFAKVRVLRETTTPKEFNAAKVSLGVLGVISQVTLKLQPMFKRSLRYV 254

Query: 262 ERDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF----- 321
            R+D+D  DQ ++FG  +EFAD  W PSQ KVVYR D RV+ NTSGNGL DF+PF     
Sbjct: 255 MRNDSDFGDQAVTFGMKHEFADFIWLPSQGKVVYRMDDRVAVNTSGNGLLDFMPFRSQLS 314

Query: 322 ---------QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQ 381
                    +E QE  RDA GKC  A +ITS L  ++YGLTNNG+ F  YPVIG  NR+ 
Sbjct: 315 AALAIIRSSEETQERFRDANGKCAGATLITSTLFATSYGLTNNGMIFTGYPVIGSQNRMM 374

Query: 382 SSGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCG 441
           SSGSCLDS++D  ITACPW+  IK EFFH+   SI L  VK FI D++ L ++E  + C 
Sbjct: 375 SSGSCLDSLHDGLITACPWDSRIKSEFFHQTTFSIPLTQVKSFINDIKSLVKIESKSLCV 434

Query: 442 LELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYG 501
           LELY+GILMRYV +S AYLGKE + ++FD TYYR+KDP+SPRL+ED +EE+EQ+ALFKY 
Sbjct: 435 LELYDGILMRYVTSSPAYLGKETEALDFDLTYYRAKDPLSPRLYEDFIEEIEQIALFKYN 494

Query: 502 GSPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDG 561
             PHWGKNRN+AF   I KYKN   FLK+K  YDP GLFS+EWT+QILG++G V+I KDG
Sbjct: 495 ALPHWGKNRNLAFDGVIKKYKNVPAFLKVKESYDPMGLFSSEWTDQILGIKGNVTIIKDG 554

Query: 562 CALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCS 581
           CALEGLC+CS+D+HCAP+KGYFCR G++YKEARVC+
Sbjct: 555 CALEGLCVCSEDAHCAPTKGYFCRPGKVYKEARVCT 590

BLAST of Carg24187 vs. TAIR 10
Match: AT2G46750.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 729.9 bits (1883), Expect = 1.6e-210
Identity = 339/575 (58.96%), Postives = 433/575 (75.30%), Query Frame = 0

Query: 22  AFRFTTFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYPT 81
           ++R    ++++  L+  + STPP+DP+KC S N NCT+TN+Y  FPDR+ CR   V YP 
Sbjct: 8   SYRTLVGLYYIFTLMHTAVSTPPDDPVKCVSGNTNCTVTNSYGAFPDRSTCRAANVAYPK 67

Query: 82  TEAEVISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMS 141
           TEAE++S VA AT+A RK++V TR+ HSI KL C DG +G+ ISTK+LN  ++ D   M+
Sbjct: 68  TEAELVSVVAAATQAGRKMRVTTRYSHSITKLVCTDGTEGLFISTKFLNHTVQADATAMT 127

Query: 142 ITVEGGVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVG 201
           +TVE G+T+R++I  AA  GLALP+APYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV  
Sbjct: 128 MTVESGMTLRQLIVEAAKVGLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTE 187

Query: 202 IKMVSPGGAQDGYAKVRRLVD--GDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFE 261
           I+MVSPG   +G+AK+R L +       NAAKVSLGVLG+I+QVT +LQPMFKRS+TY  
Sbjct: 188 IRMVSPGSVNEGFAKIRILSETTTPNEFNAAKVSLGVLGVISQVTFELQPMFKRSLTYTM 247

Query: 262 RDDTDLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF------ 321
           R+D+D  DQ ++FG+ +EFAD  W PSQ KVVYR+D RV+ NTSGNGL+DF+PF      
Sbjct: 248 RNDSDFEDQAVTFGKKHEFADFIWLPSQGKVVYRRDDRVAVNTSGNGLFDFLPFRSQLSA 307

Query: 322 --------QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQS 381
                   +E QE  RDA GKC+ A II+S L   +YGLTNNGI F  YPV+G  NR+ S
Sbjct: 308 AIAIIRTSEETQERFRDANGKCVGATIISSTLFAPSYGLTNNGIIFTGYPVVGSQNRMMS 367

Query: 382 SGSCLDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGL 441
           SGSCLDS+ D  ITAC W+  IKGEFFH+  +S+ L  VK FI D++ L ++E  + CGL
Sbjct: 368 SGSCLDSLQDGLITACAWDSRIKGEFFHQTTLSVPLTQVKSFISDIKSLVKIEQKSLCGL 427

Query: 442 ELYNGILMRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYGG 501
           EL+ GILMRYV +S AYLGKE + ++FD TYYR+KDP++PRL+ED +EE+EQ+ALFKY  
Sbjct: 428 ELHYGILMRYVTSSPAYLGKETEALDFDITYYRAKDPLTPRLYEDFIEEIEQIALFKYNA 487

Query: 502 SPHWGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDGC 561
            PHWGKNRN+AF   I KY N   FLK+K+ YDP GLFS+EWT+QILG++G  SI KDGC
Sbjct: 488 LPHWGKNRNLAFDGVIRKYNNAPAFLKVKDSYDPKGLFSSEWTDQILGIKGNASIVKDGC 547

Query: 562 ALEGLCLCSKDSHCAPSKGYFCRAGRIYKEARVCS 581
           ALEGLC+CSKD+HCAP+KGY CR G++YKEARVC+
Sbjct: 548 ALEGLCICSKDAHCAPAKGYLCRPGKVYKEARVCT 582

BLAST of Carg24187 vs. TAIR 10
Match: AT2G46740.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 716.5 bits (1848), Expect = 1.8e-206
Identity = 336/570 (58.95%), Postives = 422/570 (74.04%), Query Frame = 0

Query: 29  IFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYPTTEAEVIS 88
           ++ L  LV    STPPEDP+KC S N NC +TN+   FPDR+ CR   V YPTTEAE++S
Sbjct: 19  LYCLFTLVHTVISTPPEDPVKCVSGNTNCIVTNSLGAFPDRSTCRAANVAYPTTEAELVS 78

Query: 89  AVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVEGGV 148
            VA AT+A RK++V TR+ HSIPKL C DG DG+ ISTK+LN  ++ D + M++TVE GV
Sbjct: 79  IVAAATKAGRKMRVTTRYSHSIPKLTCTDGNDGLFISTKFLNHTVQADAKAMTLTVESGV 138

Query: 149 TVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVSPG 208
           T+R++I  AA  GLALP+APYWWG+T+GG++ TGAHGSSLWGKGSAVHDYV  I+MVSPG
Sbjct: 139 TLRQLIAEAAKVGLALPYAPYWWGVTVGGMMGTGAHGSSLWGKGSAVHDYVTEIRMVSPG 198

Query: 209 GAQDGYAKVRRLVD--GDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDTDLS 268
              DG+AK+R L +       NAAKVSLGVLG+I+QVT +LQPMFKRS+ Y  R+D D +
Sbjct: 199 SVNDGFAKIRVLSETTTPNEFNAAKVSLGVLGVISQVTFELQPMFKRSLKYVMRNDLDFN 258

Query: 269 DQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF------------- 328
           D+ ++FG+ +EFAD  W PSQ KVVYR D RV+ NT GNGLYDF PF             
Sbjct: 259 DEALTFGKKHEFADFVWLPSQGKVVYRMDDRVAVNTLGNGLYDFFPFRSQLSAVLATTRS 318

Query: 329 -QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSCLDS 388
            +E QE  RDA GKC++A  I+S L  ++YGLTNNGITF  YPVIG  NR+ SSGSCLD 
Sbjct: 319 SEETQETLRDAHGKCVTATTISSTLFSTSYGLTNNGITFTGYPVIGSQNRMMSSGSCLDG 378

Query: 389 VNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYNGIL 448
           + D   +AC W+  +KG F+H+   SI L  VK FI D++ L +++  + CGLELY GIL
Sbjct: 379 LEDKLTSACAWDSRVKGVFYHQTTFSIPLTQVKSFINDIKSLLKIDSKSLCGLELYYGIL 438

Query: 449 MRYVKASTAYLGKEEDGINFDFTYYRSKDPMSPRLFEDVLEEVEQMALFKYGGSPHWGKN 508
           MRYV +S AYLGKE + I+FD TYYR+ DP++PRL+ED +EE+EQ+AL KY   PHWGKN
Sbjct: 439 MRYVTSSPAYLGKETEAIDFDITYYRANDPLTPRLYEDFIEEIEQIALLKYNALPHWGKN 498

Query: 509 RNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDGCALEGLCL 568
           RN+AF   I KYKN   FLK+K  YDP GLFS+EWT+QILG++G  +I KDGCALEGLC+
Sbjct: 499 RNLAFDGVIKKYKNAPAFLKVKESYDPNGLFSSEWTDQILGIKGNPTIVKDGCALEGLCI 558

Query: 569 CSKDSHCAPSKGYFCRAGRIYKEARVCSYL 583
           CS D+HCAPSKGY CR G++YKEARVC+++
Sbjct: 559 CSDDAHCAPSKGYLCRPGKVYKEARVCTHV 588

BLAST of Carg24187 vs. TAIR 10
Match: AT1G32300.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 705.7 bits (1820), Expect = 3.2e-203
Identity = 339/574 (59.06%), Postives = 425/574 (74.04%), Query Frame = 0

Query: 27  TFIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYPTTEAEV 86
           + IFF     + + STPP+DP+KC S N  CT+TN+Y  FPDR+IC   +V+YP TEAE+
Sbjct: 6   SLIFF--CFCTFASSTPPDDPVKCESGNNMCTVTNSYGAFPDRSICEAAKVEYPKTEAEL 65

Query: 87  ISAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVEG 146
           +S VA ATRA +K++V TR+ HSIPKL C DG DGV+ISTK+LN V+  + E  ++TVE 
Sbjct: 66  VSIVAAATRAGQKVRVVTRYVHSIPKLVCTDGKDGVLISTKFLNNVVGTNPEAKTLTVES 125

Query: 147 GVTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVS 206
           GVT+R++I  AA   LALPHAPYWWGLT+GGL+ TGAHGSSLWGKGSAVHDYV  I+MVS
Sbjct: 126 GVTLRQLIGEAAELELALPHAPYWWGLTVGGLMGTGAHGSSLWGKGSAVHDYVSEIRMVS 185

Query: 207 PGGAQDGYAKVRRL---VDGDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDT 266
           PG A DGY KVR L   +D D+   AAKVSLGVLG+I+QVT +LQPMFKRS+T+  ++D+
Sbjct: 186 PGLASDGYVKVRVLSETIDPDE-FRAAKVSLGVLGVISQVTFQLQPMFKRSLTFVMQNDS 245

Query: 267 DLSDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF---------- 326
           D  DQ ++FG  +EFAD  W PSQ KVVYR D RV  NTSGNGL+DF PF          
Sbjct: 246 DFGDQAVTFGEKHEFADFLWLPSQGKVVYRMDDRVPVNTSGNGLFDFFPFRPQLSVALAI 305

Query: 327 ----QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSC 386
               +E +E+  DA  KC  A+ ITS L   +YG+TNNG+ F  YPVIG  N + SSG+C
Sbjct: 306 IRSLEESEESSGDANDKCARAEQITSFLFSISYGVTNNGMEFTGYPVIGKQNHMMSSGTC 365

Query: 387 LDSVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYN 446
           LDS  D  IT+CPW+P IKG+FFH+   SI L  VKGFI D++ L ++EP + C LE  N
Sbjct: 366 LDSHQDGLITSCPWDPRIKGQFFHQTAFSIPLTRVKGFINDIKALVKIEPKSLCALERSN 425

Query: 447 GILMRYVKASTAYLGKEEDGINFDFTYYRSK-DPMSPRLFEDVLEEVEQMALFKYGGSPH 506
           GIL+RYV +S A+LGKEE  ++FD TYYRSK DP++PRL+ED +EE+EQMA+FKY   PH
Sbjct: 426 GILIRYVTSSPAFLGKEEKALDFDLTYYRSKDDPLAPRLYEDFIEEIEQMAIFKYNALPH 485

Query: 507 WGKNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDGCALE 566
           WGKNRN+AF   I KYKN   FLK+K  +DP GLFS EWTNQILG++G V+I K+GCALE
Sbjct: 486 WGKNRNLAFDGVIRKYKNANTFLKVKERFDPLGLFSTEWTNQILGLKGNVTIVKEGCALE 545

Query: 567 GLCLCSKDSHCAPSKGYFCRAGRIYKEARVCSYL 583
           GLC+CS D+HCAP KGY CR G++Y +ARVC+++
Sbjct: 546 GLCVCSDDAHCAPKKGYLCRPGKVYTKARVCTHV 576

BLAST of Carg24187 vs. TAIR 10
Match: AT5G56490.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 696.0 bits (1795), Expect = 2.5e-200
Identity = 329/570 (57.72%), Postives = 422/570 (74.04%), Query Frame = 0

Query: 28  FIFFLVLLVSVSESTPPEDPIKCRSPNANCTITNAYATFPDRAICRVGEVKYPTTEAEVI 87
           F FF     + + S+PP+DP+ C   N  CT+TN+Y  FPDR+IC+  +V+YP TEAE++
Sbjct: 9   FCFF-----TFASSSPPDDPVNCEFGNTMCTVTNSYGAFPDRSICKAAKVEYPRTEAELV 68

Query: 88  SAVAEATRAMRKIKVGTRFYHSIPKLACPDGVDGVVISTKYLNRVLKVDGEGMSITVEGG 147
           S VA ATRA +K++V TR+ HS PKL C DG DG +ISTK+LN V+  + E  ++TVE G
Sbjct: 69  SIVAAATRAGQKMRVVTRYSHSFPKLVCTDGKDGTLISTKFLNHVVTTNPEAKTLTVESG 128

Query: 148 VTVREIIEVAAAGGLALPHAPYWWGLTIGGLLSTGAHGSSLWGKGSAVHDYVVGIKMVSP 207
           VT+R++IE AA   LALP+APYWWGLT+GG++ TGAHGSSLWGKGSAVHDYV  I++VSP
Sbjct: 129 VTLRQLIEEAAKFDLALPYAPYWWGLTVGGMMGTGAHGSSLWGKGSAVHDYVTEIRLVSP 188

Query: 208 GGAQDGYAKVRRLVD--GDQHLNAAKVSLGVLGIITQVTLKLQPMFKRSITYFERDDTDL 267
           G A DGY KV+ L +    +   AAKVSLGVLG+I+QVT +LQPMFKRS+ Y  R+D+D 
Sbjct: 189 GLASDGYVKVQVLSETIDPEEFRAAKVSLGVLGVISQVTFELQPMFKRSLNYVMRNDSDF 248

Query: 268 SDQVISFGRSYEFADMTWYPSQRKVVYRKDTRVSSNTSGNGLYDFIPF------------ 327
            DQ +SFG  +EFAD  W PSQ KVVYR D RV  NTSG+GL++F PF            
Sbjct: 249 GDQAVSFGERHEFADFLWLPSQGKVVYRMDGRVPLNTSGDGLFEFFPFRSQLSLVLAIDR 308

Query: 328 --QEYQEARRDAAGKCLSAKIITSLLSLSAYGLTNNGITFLKYPVIGYNNRLQSSGSCLD 387
             +E +E+  DA  KC+ AK+++S + L +YG+TNNG+ F  YPVIG  N + SSGSCLD
Sbjct: 309 SLEESEESLEDANMKCVRAKLVSSSMFLMSYGVTNNGLIFTGYPVIGMQNHMMSSGSCLD 368

Query: 388 SVNDLRITACPWEPSIKGEFFHEIGISIDLKVVKGFIEDVRKLGELEPMAFCGLELYNGI 447
           S  D  ITACPW+P IKG+FFH+   S+ L  VK FI D++ L ++EP + C LE  NGI
Sbjct: 369 SRQDGLITACPWDPRIKGQFFHQTTFSVSLTNVKSFINDIKALVKIEPKSLCVLEGSNGI 428

Query: 448 LMRYVKASTAYLGKEEDGINFDFTYYRSK-DPMSPRLFEDVLEEVEQMALFKYGGSPHWG 507
           L+RYV +S A+LGKEE  ++FD TYYRSK DP++PRL+ED +EE+EQMA+FKY   PHWG
Sbjct: 429 LIRYVTSSLAFLGKEEKALDFDLTYYRSKNDPLAPRLYEDYIEEIEQMAIFKYNALPHWG 488

Query: 508 KNRNIAFLKAIGKYKNREGFLKIKNEYDPCGLFSNEWTNQILGVEGTVSIDKDGCALEGL 567
           KNRN+AF  AI KYKN   FLK+K ++D  GLFS EWT+QILG++G V+I K GCALEGL
Sbjct: 489 KNRNLAFDGAIRKYKNANAFLKVKEKFDSLGLFSTEWTDQILGLKGNVTIVKQGCALEGL 548

Query: 568 CLCSKDSHCAPSKGYFCRAGRIYKEARVCS 581
           C+CS+DSHCAP+KGY CR G++Y+EARVC+
Sbjct: 549 CICSEDSHCAPTKGYLCRPGKVYREARVCT 573

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7028488.10.0e+00100.00putative L-gulonolactone oxidase 6, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_023540226.10.0e+0096.23probable L-gulonolactone oxidase 6 [Cucurbita pepo subsp. pepo][more]
XP_022943972.10.0e+0095.71probable L-gulonolactone oxidase 6 [Cucurbita moschata][more]
XP_022975260.10.0e+0094.17LOW QUALITY PROTEIN: probable L-gulonolactone oxidase 6 [Cucurbita maxima][more]
KAG6597012.10.0e+0096.79putative L-gulonolactone oxidase 6, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
O810322.8e-21261.46Probable L-gulonolactone oxidase 6 OS=Arabidopsis thaliana OX=3702 GN=GULLO6 PE=... [more]
Q6NQ662.2e-20958.96L-gulonolactone oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GULLO2 PE=1 SV=1[more]
O810302.5e-20558.95L-gulonolactone oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=GULLO5 PE=1 SV=1[more]
Q9C6144.5e-20259.06Probable L-gulonolactone oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GULLO1 PE=... [more]
Q9FM823.5e-19957.72Probable L-gulonolactone oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GULLO4 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1FUH80.0e+0095.71L-gulonolactone oxidase OS=Cucurbita moschata OX=3662 GN=LOC111448536 PE=3 SV=1[more]
A0A6J1IG870.0e+0094.17L-gulonolactone oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474382 PE=3 SV=1[more]
A0A1S3AUG32.2e-26076.98L-gulonolactone oxidase OS=Cucumis melo OX=3656 GN=LOC103483108 PE=3 SV=1[more]
A0A0A0L3K41.4e-25475.09L-gulonolactone oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G116800 PE=3 SV=1[more]
A0A2P5A4Y91.1e-22766.08L-gulonolactone oxidase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_368830 PE... [more]
Match NameE-valueIdentityDescription
AT2G46760.12.0e-21361.46D-arabinono-1,4-lactone oxidase family protein [more]
AT2G46750.11.6e-21058.96D-arabinono-1,4-lactone oxidase family protein [more]
AT2G46740.11.8e-20658.95D-arabinono-1,4-lactone oxidase family protein [more]
AT1G32300.13.2e-20359.06D-arabinono-1,4-lactone oxidase family protein [more]
AT5G56490.12.5e-20057.72D-arabinono-1,4-lactone oxidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 131..249
e-value: 8.1E-31
score: 108.3
IPR007173D-arabinono-1,4-lactone oxidasePFAMPF04030ALOcoord: 243..530
e-value: 3.5E-13
score: 49.9
IPR010030L-gulonolactone oxidase, plantTIGRFAMTIGR01677TIGR01677coord: 43..579
e-value: 2.1E-247
score: 820.1
NoneNo IPR availableGENE3D3.30.70.2520coord: 394..494
e-value: 1.2E-5
score: 27.5
NoneNo IPR availablePANTHERPTHR13878:SF93D-ARABINONO-1,4-LACTONE OXIDASE-LIKE PROTEINcoord: 30..582
NoneNo IPR availablePANTHERPTHR13878GULONOLACTONE OXIDASEcoord: 30..582
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 78..209
e-value: 1.5E-17
score: 63.6
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 63..250
score: 19.950848
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 61..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24187-RACarg24187-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019853 L-ascorbic acid biosynthetic process
cellular_component GO:0016020 membrane
molecular_function GO:0003885 D-arabinono-1,4-lactone oxidase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0050105 L-gulonolactone oxidase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity