Carg24112 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24112
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	alpha/beta-Hydrolases superfamily protein
Location	Carg_Chr09: 11207832 .. 11208857 (-)
RNA-Seq Expression	Carg24112
Synteny	Carg24112

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGCCGTTCAGTGTTTCGTCGGAGATTTCCACCTCCCAACTTCCATGGAAGCACCGCCTTACTCTCCGATTCGCCGCCCTACTCTTCAACGCTTCTATCCGATCAGATCTCACCGTTAATCGCCGGCTTCTCAATTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAATCCACGCCGTTTCCTCTTCCGATCTCACAATCGACGACTCTCGTGACCTCTTCCTCCGAATCTTCACCCCCACCGTCGCCGCCACGGACTCAGTCCTGCCGCCGGTAATTTTCTACTTTCACGGCGGCGGTTTCGCCTTCGGTTCTGCCGCCGCCACGTCAATGGACATGGCCGCCCGCCGTTTCAGTAAGGAACTCGGAGCTGTCGTAATCTCCGTGAATTACCGTCTCGCTCCTCAGTTCCGATTCCCTTGCCAATACGACGACGGATTTGACGCCGTGAAATTCATCGACGAAATGGACGACGACGATCCATTGTTGGGTAAGGCAGATCTGAGCCGTTGCTTTATTTTGGGGGAAAGCGCCGGGGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGCTTTTAAGAGAGTGAAGGTAATTGGGTTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATCAGCCTCCGTTAACTCTGAAACTTGCGGATTGGTTCTGGAAGGCGTTTTTGCCGGCGGGGGAAGACCGGGATCACGCGGCGGCGAATGTGAATGGACCGAACGGAAGGGATGTCTCCGGACTGAGAAATTTTCCGGCGACGATTATTTTGGCCGGAGGATTGGATATTCTGATAGATCGGCAGAGGAGGTACTACGAGGAGCTGAAGAGAATGGGAAAGGAGGTGAAATTGGTGGTGTTTACGAACGGCGTCCATGGATTCTTTAGCTTTGAGGATCTGCCGGAATATTCTCTAATGATTAAGGAAATGAGGGATTTCATTGCAATGTTTCACCGGAATATTCTTTAA

mRNA sequence

ATGCAGCCGTTCAGTGTTTCGTCGGAGATTTCCACCTCCCAACTTCCATGGAAGCACCGCCTTACTCTCCGATTCGCCGCCCTACTCTTCAACGCTTCTATCCGATCAGATCTCACCGTTAATCGCCGGCTTCTCAATTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAATCCACGCCGTTTCCTCTTCCGATCTCACAATCGACGACTCTCGTGACCTCTTCCTCCGAATCTTCACCCCCACCGTCGCCGCCACGGACTCAGTCCTGCCGCCGGTAATTTTCTACTTTCACGGCGGCGGTTTCGCCTTCGGTTCTGCCGCCGCCACGTCAATGGACATGGCCGCCCGCCGTTTCAGTAAGGAACTCGGAGCTGTCGTAATCTCCGTGAATTACCGTCTCGCTCCTCAGTTCCGATTCCCTTGCCAATACGACGACGGATTTGACGCCGTGAAATTCATCGACGAAATGGACGACGACGATCCATTGTTGGGTAAGGCAGATCTGAGCCGTTGCTTTATTTTGGGGGAAAGCGCCGGGGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGCTTTTAAGAGAGTGAAGGTAATTGGGTTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATCAGCCTCCGTTAACTCTGAAACTTGCGGATTGGTTCTGGAAGGCGTTTTTGCCGGCGGGGGAAGACCGGGATCACGCGGCGGCGAATGTGAATGGACCGAACGGAAGGGATGTCTCCGGACTGAGAAATTTTCCGGCGACGATTATTTTGGCCGGAGGATTGGATATTCTGATAGATCGGCAGAGGAGGTACTACGAGGAGCTGAAGAGAATGGGAAAGGAGGTGAAATTGGTGGTGTTTACGAACGGCGTCCATGGATTCTTTAGCTTTGAGGATCTGCCGGAATATTCTCTAATGATTAAGGAAATGAGGGATTTCATTGCAATGTTTCACCGGAATATTCTTTAA

Coding sequence (CDS)

ATGCAGCCGTTCAGTGTTTCGTCGGAGATTTCCACCTCCCAACTTCCATGGAAGCACCGCCTTACTCTCCGATTCGCCGCCCTACTCTTCAACGCTTCTATCCGATCAGATCTCACCGTTAATCGCCGGCTTCTCAATTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAATCCACGCCGTTTCCTCTTCCGATCTCACAATCGACGACTCTCGTGACCTCTTCCTCCGAATCTTCACCCCCACCGTCGCCGCCACGGACTCAGTCCTGCCGCCGGTAATTTTCTACTTTCACGGCGGCGGTTTCGCCTTCGGTTCTGCCGCCGCCACGTCAATGGACATGGCCGCCCGCCGTTTCAGTAAGGAACTCGGAGCTGTCGTAATCTCCGTGAATTACCGTCTCGCTCCTCAGTTCCGATTCCCTTGCCAATACGACGACGGATTTGACGCCGTGAAATTCATCGACGAAATGGACGACGACGATCCATTGTTGGGTAAGGCAGATCTGAGCCGTTGCTTTATTTTGGGGGAAAGCGCCGGGGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGCTTTTAAGAGAGTGAAGGTAATTGGGTTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATCAGCCTCCGTTAACTCTGAAACTTGCGGATTGGTTCTGGAAGGCGTTTTTGCCGGCGGGGGAAGACCGGGATCACGCGGCGGCGAATGTGAATGGACCGAACGGAAGGGATGTCTCCGGACTGAGAAATTTTCCGGCGACGATTATTTTGGCCGGAGGATTGGATATTCTGATAGATCGGCAGAGGAGGTACTACGAGGAGCTGAAGAGAATGGGAAAGGAGGTGAAATTGGTGGTGTTTACGAACGGCGTCCATGGATTCTTTAGCTTTGAGGATCTGCCGGAATATTCTCTAATGATTAAGGAAATGAGGGATTTCATTGCAATGTTTCACCGGAATATTCTTTAA

Protein sequence

MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL

Homology

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BLAST of Carg24112 vs. NCBI nr
Match: KAG6592331.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025153.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 691.8 bits (1784), Expect = 3.0e-195
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0


Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120
           AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRF
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120

Query: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180
           SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240
           GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKE 300
           AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKE
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKE 300

Query: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 342
           VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL
Sbjct: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 341

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BLAST of Carg24112 vs. NCBI nr
Match: XP_022925491.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 682.9 bits (1761), Expect = 1.4e-192
Identity = 337/341 (98.83%), Postives = 338/341 (99.12%), Query Frame = 0


Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQP SVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120
           AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSA ATSMDMAARRF
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSADATSMDMAARRF 120

Query: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180
           SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240
           GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKE 300
           AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLD+LIDRQRRYYEELKRMGKE
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKE 300

Query: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 342
           VKLVVFTNGVHGFFSFED PEYSLMIKEMRDFIAMFHRNIL
Sbjct: 301 VKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIAMFHRNIL 341

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BLAST of Carg24112 vs. NCBI nr
Match: XP_022973499.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 679.5 bits (1752), Expect = 1.5e-191
Identity = 333/341 (97.65%), Postives = 338/341 (99.12%), Query Frame = 0


Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120
           AVSSSDLTID SRDLFLRIFTPTVAATDS+LPPVIFYFHGGGFAFGSAAATSMDMAARRF
Sbjct: 61  AVSSSDLTIDASRDLFLRIFTPTVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120

Query: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180
           SKELGAVVISVNYRL+PQFRFPCQYDDGFDAVKFIDEMDD+DPLLG+ADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240
           GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKE 300
           AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLD LIDRQRRYYEELKRMGK+
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYEELKRMGKD 300

Query: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 342
           VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI MFHRNIL
Sbjct: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIGMFHRNIL 341

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BLAST of Carg24112 vs. NCBI nr
Match: XP_023536262.1 (probable carboxylesterase 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 678.7 bits (1750), Expect = 2.6e-191
Identity = 336/349 (96.28%), Postives = 340/349 (97.42%), Query Frame = 0


Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQPFSVSSEISTSQLPWKHRLTLRFAALLFNAS+RSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDDSRDLFLRIFTPT--------VAATDSVLPPVIFYFHGGGFAFGSAAATS 120
           AVSSSDLTID SRDLFLRIFTPT        VAATDS+LPPVIFYFHGGGFAFGSAAATS
Sbjct: 61  AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATS 120

Query: 121 MDMAARRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180
           MDMAARRFSKELGAV+ISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR
Sbjct: 121 MDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180

Query: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240
           CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL
Sbjct: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240

Query: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYE 300
           KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLD+LIDRQRRYYE
Sbjct: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300

Query: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 342
           ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL
Sbjct: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 349

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BLAST of Carg24112 vs. NCBI nr
Match: XP_004141618.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein Csa_008680 [Cucumis sativus])

HSP 1 Score: 550.1 bits (1416), Expect = 1.4e-152
Identity = 270/337 (80.12%), Postives = 295/337 (87.54%), Query Frame = 0


Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           M+  SV  E+ST QLPWKH++TLRFA LLFN S+RSD TVNRRLL FLDPKIPPNPNS H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  AVSSSDLTIDDSRDLFLRIFTPT-VAATDSVLP--PVIFYFHGGGFAFGSAAATSMDMAA 120
           +VSSSDLTID SRDLFLRIFTP   AA D  LP  P+IFYFHGGGFAFGSA ATS DMAA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 RRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILG 180
           R F+++L AVVISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDDD  LL + DLSRCFILG
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDS-LLERVDLSRCFILG 180

Query: 181 ESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADW 240
           ESAGGNLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PLTL + DW
Sbjct: 181 ESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDW 240

Query: 241 FWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRM 300
           FW+AFLPAGEDRDHAAANVNGPNGRD+SGL NFPAT+I AGGLD+L+DRQ+ YYE LKRM
Sbjct: 241 FWRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRM 300

Query: 301 GKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 335
           GK+VKLVVF+N  HGFF F DLPEYSLMI+EM DFIA
Sbjct: 301 GKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIA 336

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BLAST of Carg24112 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 6.2e-79
Identity = 155/322 (48.14%), Postives = 209/322 (64.91%), Query Frame = 0


Query: 15  LPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDDSRD 74
           LP K R+ L   + + + + R D T+NRR L   D + PPNP  ++ VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVVISVNYR 134
           L+ R++TP V+  D +  PV+ +FHGGGFAF S  A   D   RRF+++L A VISVNYR
Sbjct: 73  LWFRLYTPHVSG-DKI--PVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYR 132

Query: 135 LAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGHHVAV---R 194
           LAP+ R+P QYDDGFDA+K+I+E +    L   ADLSRCF  G+SAGGN+ H+VA+   R
Sbjct: 133 LAPEHRYPAQYDDGFDALKYIEE-NHGSILPANADLSRCFFAGDSAGGNIAHNVAIRICR 192

Query: 195 ASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDH 254
               +F  VK+IG I+ QPFFGGEERTE+E +L   P ++    DW WKA    G +RDH
Sbjct: 193 EPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRDH 252

Query: 255 AAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVFTNGVH 314
            A NV GPN  D+SGL ++P T+++  G D L D QR YYE LK  GK+  L+ + N  H
Sbjct: 253 EAVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMFH 312

Query: 315 GFFSFEDLPEYSLMIKEMRDFI 334
            F+ F +LPE   +I  ++DF+
Sbjct: 313 AFYIFPELPEAGQLIMRIKDFV 326

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BLAST of Carg24112 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 9.9e-61
Identity = 130/318 (40.88%), Postives = 184/318 (57.86%), Query Frame = 0


Query: 28  LLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDDSRDLFLRIFTPTVAAT 87
           L +N   R D T NR L  FLD K+P N N ++ V S D+ ID   +L  R++ P  A T
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  -----------DSVLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVVISVNYRLA 147
                      D  + PVI +FHGG FA  SA +   D   RR     GAVV+SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLS-RCFILGESAGGNLGHHVAVRASEY 207
           P+ R+PC YDDG+  +K++   +    L  K D   R F+ G+S+GGN+ H+VAVRA E 
Sbjct: 149 PENRYPCAYDDGWAVLKWV---NSSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE- 208

Query: 208 AFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAAN 267
              R+ V+G I   P FGG ERTESE RL  +  +T++  DW+W+AFLP GEDR+H A +
Sbjct: 209 --SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACS 268

Query: 268 VNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFS 327
             GP  + + GL +FP ++++  GLD++ D Q +Y E LK+ G+EVKL+       GF+ 
Sbjct: 269 PFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYL 328

Query: 328 FEDLPEYSLMIKEMRDFI 334
             +   +  ++ E+  F+
Sbjct: 329 LPNNNHFHTVMDEIAAFV 339

[Close]

BLAST of Carg24112 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.8e-57
Identity = 133/326 (40.80%), Postives = 180/326 (55.21%), Query Frame = 0


Query: 28  LLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDDSRDLFLRIFTP----- 87
           L +N   R+D T  R L  +LD ++P N   +  VSS D  ID S  L +RI+       
Sbjct: 29  LSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGD 88

Query: 88  ----TVAATDSVLP-----------PVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAV 147
                 A T  +L            PVI +FHGG F   SA++T  D   RRF K    V
Sbjct: 89  AEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGV 148

Query: 148 VISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGHH 207
           V+SVNYR AP+ R+PC YDDG+ A+K++  M       G    +R F+ G+S+GGN+ HH
Sbjct: 149 VVSVNYRRAPEHRYPCAYDDGWTALKWV--MSQPFMRSGGDAQARVFLSGDSSGGNIAHH 208

Query: 208 VAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGE 267
           VAVRA++   + VKV G I     FGG ERTESE RL  +  +TL+  DW+WKA+LP   
Sbjct: 209 VAVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDA 268

Query: 268 DRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVFT 327
           DRDH A N  GPNGR + GL  F  ++I+  GLD+  DRQ  Y + L+  G  VK+V   
Sbjct: 269 DRDHPACNPFGPNGRRLGGL-PFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCE 328

Query: 328 NGVHGFFSFEDLPEYSLMIKEMRDFI 334
           N   GF+   +   Y  +++E+ DF+
Sbjct: 329 NATVGFYLLPNTVHYHEVMEEISDFL 348

[Close]

BLAST of Carg24112 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.2e-53
Identity = 120/318 (37.74%), Postives = 173/318 (54.40%), Query Frame = 0


Query: 30  FNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDDSRDLFLRIFTPTVA---- 89
           +N   R D T NR L  +LD K+  N N +  V S D+ ID   +L  R++ P  A    
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  ---------ATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVVISVNYRLA 149
                      D  + PVI +FHGG FA  SA +   D   RR       VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLS-RCFILGESAGGNLGHHVAVRASEY 209
           P+  +PC YDDG+ A+ +++       L  K D     F+ G+S+GGN+ H+VA+RA E 
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR---SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE- 210

Query: 210 AFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAAN 269
               + V+G I   P FGG ERTESE  L  +  +T++  DW+WKAFLP GEDR+H A N
Sbjct: 211 --SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACN 270

Query: 270 VNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFS 329
              P G+ + G+ +FP ++++  GLD++ D Q  Y E LK+ G+EVKL+       GF+ 
Sbjct: 271 PFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYL 330

Query: 330 FEDLPEYSLMIKEMRDFI 334
             +   +  ++ E+  F+
Sbjct: 331 LPNNNHFHNVMDEISAFV 341

[Close]

BLAST of Carg24112 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.9e-52
Identity = 115/320 (35.94%), Postives = 177/320 (55.31%), Query Frame = 0


Query: 28  LLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDDSRDLFLRIFTP----- 87
           L +    R D + NR L  FLD K+P N   +  V S D  +D + +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ---------TVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVVISVNY 147
                    T   + + + PV+ +FHGG F   SA +   D   RR     G VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGHHVAVRAS 207
           R +P+ R+PC YDDG++A+ ++          GK      ++ G+S+GGN+ H+VAVRA+
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRVWLQS--GKDSNVYVYLAGDSSGGNIAHNVAVRAT 208

Query: 208 EYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAA 267
               + VKV+G I   P FGG+ERT+SE  L  +  +T++  DW+W+A+LP GEDRDH A
Sbjct: 209 N---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPA 268

Query: 268 ANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVFTNGVHGF 327
            N  GP G+ + G+ NFP ++++  GLD++ D Q  Y + LK+ G EV L+       GF
Sbjct: 269 CNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGF 328

Query: 328 FSFEDLPEYSLMIKEMRDFI 334
           +   +   +  +++E+  F+
Sbjct: 329 YFLPNNDHFHCLMEELNKFV 341

[Close]

BLAST of Carg24112 vs. ExPASy TrEMBL
Match: A0A6J1ECC8 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 6.6e-193
Identity = 337/341 (98.83%), Postives = 338/341 (99.12%), Query Frame = 0


Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQP SVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120
           AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSA ATSMDMAARRF
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSADATSMDMAARRF 120

Query: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180
           SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240
           GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKE 300
           AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLD+LIDRQRRYYEELKRMGKE
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKE 300

Query: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 342
           VKLVVFTNGVHGFFSFED PEYSLMIKEMRDFIAMFHRNIL
Sbjct: 301 VKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIAMFHRNIL 341

[Close]

BLAST of Carg24112 vs. ExPASy TrEMBL
Match: A0A6J1IER7 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 7.3e-192
Identity = 333/341 (97.65%), Postives = 338/341 (99.12%), Query Frame = 0


Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120
           AVSSSDLTID SRDLFLRIFTPTVAATDS+LPPVIFYFHGGGFAFGSAAATSMDMAARRF
Sbjct: 61  AVSSSDLTIDASRDLFLRIFTPTVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120

Query: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180
           SKELGAVVISVNYRL+PQFRFPCQYDDGFDAVKFIDEMDD+DPLLG+ADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240
           GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKE 300
           AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLD LIDRQRRYYEELKRMGK+
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYEELKRMGKD 300

Query: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 342
           VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI MFHRNIL
Sbjct: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIGMFHRNIL 341

[Close]

BLAST of Carg24112 vs. ExPASy TrEMBL
Match: A0A0A0KXS8 (Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.7e-153
Identity = 270/337 (80.12%), Postives = 295/337 (87.54%), Query Frame = 0


Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           M+  SV  E+ST QLPWKH++TLRFA LLFN S+RSD TVNRRLL FLDPKIPPNPNS H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  AVSSSDLTIDDSRDLFLRIFTPT-VAATDSVLP--PVIFYFHGGGFAFGSAAATSMDMAA 120
           +VSSSDLTID SRDLFLRIFTP   AA D  LP  P+IFYFHGGGFAFGSA ATS DMAA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 RRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILG 180
           R F+++L AVVISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDDD  LL + DLSRCFILG
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDS-LLERVDLSRCFILG 180

Query: 181 ESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADW 240
           ESAGGNLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PLTL + DW
Sbjct: 181 ESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDW 240

Query: 241 FWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRM 300
           FW+AFLPAGEDRDHAAANVNGPNGRD+SGL NFPAT+I AGGLD+L+DRQ+ YYE LKRM
Sbjct: 241 FWRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRM 300

Query: 301 GKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 335
           GK+VKLVVF+N  HGFF F DLPEYSLMI+EM DFIA
Sbjct: 301 GKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIA 336

[Close]

BLAST of Carg24112 vs. ExPASy TrEMBL
Match: A0A1S3CBA4 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.3e-151
Identity = 268/332 (80.72%), Postives = 293/332 (88.25%), Query Frame = 0


Query: 6   VSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSS 65
           V  +IST QLPWKHR+TLRFA LL+N S+RSDLTVNRRLLNF+DPKIP N +S H+VSSS
Sbjct: 6   VCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTHSVSSS 65

Query: 66  DLTIDDSRDLFLRIFTPT-VAATDSVLP--PVIFYFHGGGFAFGSAAATSMDMAARRFSK 125
           DL +D SRDLFLRIFTPT  AA D  LP  P+IFYFHGGGF FGSA ATS DMAARRF+K
Sbjct: 66  DLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAARRFAK 125

Query: 126 ELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGG 185
           +L AVVISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDDD  LL + DLS CFILGESAGG
Sbjct: 126 KLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDS-LLERVDLSCCFILGESAGG 185

Query: 186 NLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAF 245
           NLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PLTL + DWFW+AF
Sbjct: 186 NLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWFWRAF 245

Query: 246 LPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVK 305
           LPAGEDRDHAAANVNGPNGRD+SGL NFPAT+I AGGLD+LIDRQ+ YYE LKRMGK+VK
Sbjct: 246 LPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMGKDVK 305

Query: 306 LVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 335
           LVVF+N VHG+F F DLPEYSLMIKEMRDFIA
Sbjct: 306 LVVFSNAVHGYFGFADLPEYSLMIKEMRDFIA 336

[Close]

BLAST of Carg24112 vs. ExPASy TrEMBL
Match: A0A5D3BL91 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001330 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.4e-150
Identity = 266/332 (80.12%), Postives = 291/332 (87.65%), Query Frame = 0


Query: 6   VSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSS 65
           V  +IST QLPWKHR+TLRFA LL+N S+RSDLTVNRRLLNF+DPKIP N +S H+VSSS
Sbjct: 6   VCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTHSVSSS 65

Query: 66  DLTIDDSRDLFLRIFTPT-VAATDSVLP--PVIFYFHGGGFAFGSAAATSMDMAARRFSK 125
           DL +D SRDLFLRIFTPT  AA D  LP  P+IFYFHGGGF FGSA ATS DMAARRF+K
Sbjct: 66  DLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAARRFAK 125

Query: 126 ELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGG 185
           +L AVVISVNYRLAP+FRFPCQ DDGFD +KFIDEMDDD  LL + DLSRCFILGESAGG
Sbjct: 126 KLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDS-LLERVDLSRCFILGESAGG 185

Query: 186 NLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAF 245
           NLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PLTL + DWFW+AF
Sbjct: 186 NLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWFWRAF 245

Query: 246 LPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVK 305
           LPAGEDRDHA ANVNGPNGRD+SGL NFPAT+I AGGLD+LIDRQ+ YYE LKRMGK+VK
Sbjct: 246 LPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMGKDVK 305

Query: 306 LVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 335
           LVVF+N VHG+F F DLPEYSLMIKEMRDFIA
Sbjct: 306 LVVFSNAVHGYFGFADLPEYSLMIKEMRDFIA 336

[Close]

BLAST of Carg24112 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 295.8 bits (756), Expect = 4.4e-80
Identity = 155/322 (48.14%), Postives = 209/322 (64.91%), Query Frame = 0


Query: 15  LPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDDSRD 74
           LP K R+ L   + + + + R D T+NRR L   D + PPNP  ++ VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVVISVNYR 134
           L+ R++TP V+  D +  PV+ +FHGGGFAF S  A   D   RRF+++L A VISVNYR
Sbjct: 73  LWFRLYTPHVSG-DKI--PVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYR 132

Query: 135 LAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGHHVAV---R 194
           LAP+ R+P QYDDGFDA+K+I+E +    L   ADLSRCF  G+SAGGN+ H+VA+   R
Sbjct: 133 LAPEHRYPAQYDDGFDALKYIEE-NHGSILPANADLSRCFFAGDSAGGNIAHNVAIRICR 192

Query: 195 ASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDH 254
               +F  VK+IG I+ QPFFGGEERTE+E +L   P ++    DW WKA    G +RDH
Sbjct: 193 EPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRDH 252

Query: 255 AAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVFTNGVH 314
            A NV GPN  D+SGL ++P T+++  G D L D QR YYE LK  GK+  L+ + N  H
Sbjct: 253 EAVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMFH 312

Query: 315 GFFSFEDLPEYSLMIKEMRDFI 334
            F+ F +LPE   +I  ++DF+
Sbjct: 313 AFYIFPELPEAGQLIMRIKDFV 326

[Close]

BLAST of Carg24112 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 235.3 bits (599), Expect = 7.1e-62
Identity = 130/318 (40.88%), Postives = 184/318 (57.86%), Query Frame = 0


Query: 28  LLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDDSRDLFLRIFTPTVAAT 87
           L +N   R D T NR L  FLD K+P N N ++ V S D+ ID   +L  R++ P  A T
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  -----------DSVLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVVISVNYRLA 147
                      D  + PVI +FHGG FA  SA +   D   RR     GAVV+SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLS-RCFILGESAGGNLGHHVAVRASEY 207
           P+ R+PC YDDG+  +K++   +    L  K D   R F+ G+S+GGN+ H+VAVRA E 
Sbjct: 149 PENRYPCAYDDGWAVLKWV---NSSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE- 208

Query: 208 AFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAAN 267
              R+ V+G I   P FGG ERTESE RL  +  +T++  DW+W+AFLP GEDR+H A +
Sbjct: 209 --SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACS 268

Query: 268 VNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFS 327
             GP  + + GL +FP ++++  GLD++ D Q +Y E LK+ G+EVKL+       GF+ 
Sbjct: 269 PFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYL 328

Query: 328 FEDLPEYSLMIKEMRDFI 334
             +   +  ++ E+  F+
Sbjct: 329 LPNNNHFHTVMDEIAAFV 339

[Close]

BLAST of Carg24112 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 211.8 bits (538), Expect = 8.4e-55
Identity = 120/318 (37.74%), Postives = 173/318 (54.40%), Query Frame = 0


Query: 30  FNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDDSRDLFLRIFTPTVA---- 89
           +N   R D T NR L  +LD K+  N N +  V S D+ ID   +L  R++ P  A    
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  ---------ATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVVISVNYRLA 149
                      D  + PVI +FHGG FA  SA +   D   RR       VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLS-RCFILGESAGGNLGHHVAVRASEY 209
           P+  +PC YDDG+ A+ +++       L  K D     F+ G+S+GGN+ H+VA+RA E 
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR---SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE- 210

Query: 210 AFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAAN 269
               + V+G I   P FGG ERTESE  L  +  +T++  DW+WKAFLP GEDR+H A N
Sbjct: 211 --SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACN 270

Query: 270 VNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFS 329
              P G+ + G+ +FP ++++  GLD++ D Q  Y E LK+ G+EVKL+       GF+ 
Sbjct: 271 PFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYL 330

Query: 330 FEDLPEYSLMIKEMRDFI 334
             +   +  ++ E+  F+
Sbjct: 331 LPNNNHFHNVMDEISAFV 341

[Close]

BLAST of Carg24112 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.1e-53
Identity = 115/320 (35.94%), Postives = 177/320 (55.31%), Query Frame = 0


Query: 28  LLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDDSRDLFLRIFTP----- 87
           L +    R D + NR L  FLD K+P N   +  V S D  +D + +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ---------TVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVVISVNY 147
                    T   + + + PV+ +FHGG F   SA +   D   RR     G VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGHHVAVRAS 207
           R +P+ R+PC YDDG++A+ ++          GK      ++ G+S+GGN+ H+VAVRA+
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRVWLQS--GKDSNVYVYLAGDSSGGNIAHNVAVRAT 208

Query: 208 EYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAA 267
               + VKV+G I   P FGG+ERT+SE  L  +  +T++  DW+W+A+LP GEDRDH A
Sbjct: 209 N---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPA 268

Query: 268 ANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVFTNGVHGF 327
            N  GP G+ + G+ NFP ++++  GLD++ D Q  Y + LK+ G EV L+       GF
Sbjct: 269 CNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGF 328

Query: 328 FSFEDLPEYSLMIKEMRDFI 334
           +   +   +  +++E+  F+
Sbjct: 329 YFLPNNDHFHCLMEELNKFV 341

[Close]

BLAST of Carg24112 vs. TAIR 10
Match: AT2G45600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 169.1 bits (427), Expect = 6.2e-42
Identity = 103/298 (34.56%), Postives = 156/298 (52.35%), Query Frame = 0


Query: 31  NASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDDSRDLFLRIFTPTVAATDSV 90
           N ++ SD ++ R   +F  PK+PP         S D+ ++ + + F+RIF P     +S 
Sbjct: 15  NITLNSDGSLTRH-RDF--PKLPPTE------QSKDIPLNQTNNTFIRIFKPRNIPPESK 74

Query: 91  LPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVVISVNYRLAPQFRFPCQYDDGFD 150
           L P++ YFHGGGF   SAA+     +  + +  L  +++SV YRLAP+ R P  Y+D  +
Sbjct: 75  L-PILVYFHGGGFILYSAASAPFHESCTKMADRLQTIILSVEYRLAPEHRLPAAYEDAVE 134

Query: 151 AVKFIDEM--------DDDDPLLGKADLSRCFILGESAGGNLGHHVAVRASEYAFKRVKV 210
           A+ ++ +         D D  L    D S+C+++G S+GGN+ ++VA+R  +     VK+
Sbjct: 135 AILWLRDQARGPINGGDCDTWLKDGVDFSKCYVMGSSSGGNIVYNVALRVVDTDLSPVKI 194

Query: 211 IGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAAN---VNGP 270
            G I +Q FFGG E ++SE RL +     L      W   LP G DRDH  +N    +GP
Sbjct: 195 QGLIMNQAFFGGVEPSDSESRLKDDKICPLPATHLLWSLCLPDGVDRDHVYSNPIKSSGP 254

Query: 271 NGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFSFE 318
             +D  G   FP+T+I   G D L+DRQR   E LK  G  V+     +G H    F+
Sbjct: 255 QEKDKMG--RFPSTLINGYGGDPLVDRQRHVAEMLKGRGVHVETRFDKDGFHACELFD 300

The following BLAST results are available for this feature:

BLAST of Carg24112 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6592331.1	3.0e-195	100.00	putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_022925491.1	1.4e-192	98.83	probable carboxylesterase 18 [Cucurbita moschata]	[more]
XP_022973499.1	1.5e-191	97.65	probable carboxylesterase 18 [Cucurbita maxima]	[more]
XP_023536262.1	2.6e-191	96.28	probable carboxylesterase 18 [Cucurbita pepo subsp. pepo]	[more]
XP_004141618.1	1.4e-152	80.12	probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein ...	[more]

BLAST of Carg24112 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LT10	6.2e-79	48.14	Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1	[more]
Q940G6	9.9e-61	40.88	Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1	[more]
Q6L545	1.8e-57	40.80	Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ...	[more]
Q9MAA7	1.2e-53	37.74	Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1	[more]
Q9LYC1	2.9e-52	35.94	Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1	[more]

BLAST of Carg24112 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ECC8	6.6e-193	98.83	probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 ...	[more]
A0A6J1IER7	7.3e-192	97.65	probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV...	[more]
A0A0A0KXS8	6.7e-153	80.12	Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1	[more]
A0A1S3CBA4	1.3e-151	80.72	probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1	[more]
A0A5D3BL91	2.4e-150	80.12	Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]

BLAST of Carg24112 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23530.1	4.4e-80	48.14	carboxyesterase 18	[more]
AT5G27320.1	7.1e-62	40.88	alpha/beta-Hydrolases superfamily protein	[more]
AT3G05120.1	8.4e-55	37.74	alpha/beta-Hydrolases superfamily protein	[more]
AT3G63010.1	2.1e-53	35.94	alpha/beta-Hydrolases superfamily protein	[more]
AT2G45600.1	6.2e-42	34.56	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 95..314 e-value: 6.0E-51 score: 173.3
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 4..334 e-value: 4.7E-87 score: 294.4
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 62..333
None	No IPR available	PANTHER	PTHR23024:SF434	ACETYL ESTERASE	coord: 10..336
None	No IPR available	PANTHER	PTHR23024	ARYLACETAMIDE DEACETYLASE	coord: 10..336
IPR002168	Lipase, GDXG, putative histidine active site	PROSITE	PS01173	LIPASE_GDXG_HIS	coord: 95..111

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24112-RA	Carg24112-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity