Carg24062 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24062
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationCarg_Chr10: 5966735 .. 5971043 (+)
RNA-Seq ExpressionCarg24062
SyntenyCarg24062
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTTTCTCTGCGTCCTTCGGGTAGGTTTGGCGGCCGATCTCAACTCCTCCGCGCTATTTCGGTTCCATGTTATACATTTCACAGCGTCCTAGGAATATTCATCTAAACTCAAAGAGCTGCACATGGATGCTGGTGGATCCTAATGCGAAAAAACGAAGCTTTTAATGACAACAGTTTGGATGTGTTCAAGCGGACGCATTCGTGGAGAGTATTGGAGGAGCAGTTTGGGATGGAGGAATATGTATTCATTTCAAACCTTGCGTTTGCGTTCTTCACAGGAAAACAAGGTAAATTTCAATTGAAGATTAAAACTAAAGCTAGCCTTTGTGCTGTAATTAGAATTCTTTTTACCTAATCTGACTCTGCCAATTGTTTTCCATTCTGCATTTTTTTTTATATGTTATTAAGTCCTGGTGTAATGTAATGGTTGAATTCCATCAACAAAGGTGAAACTGCATTTTCAATCCACTTTTGGTCTGAATTGTATTAGCGATTAGAATTCCATCAGTTGCAGGTTGGAGTTTGGTCTTCTGTGGATGTTCGGCTGAGTATTGAGGGGGGTGGATAACATGAACAAGAGTAAGGAGCATAGATTTTTGGGATATAGGATCTGTGAAACCATTAATAGAGTTGTGGATCATCTCACGTACATAGCAACTATTTTAGTAGCCTATGACCATGTGGTTGTTTTCTAATTTCTGGGATGGTAATGAAAAACTAATTCTGGTTTACATCAACAGATTTGGATCCCAGCTAGTTGTATTTCCAGAAGCCTTTATTGGTGGTTATCCCCGTGGATCAAATTTTGGTGTTTCCATTTGGAATACAACTCCCAAGGGAGGAATTCCGCAACCAGGATGGTTCATTACTTGGCTAATCAAAAACGGACAAATATCATGCACCAGCGGTCGATGTACCTGGTTAGTCTTTGTTTCACTTAATTTTGGTTGCTCCTGTGTAAAGAGTGATATTAGTGTCTTACCATGGATGATCTCAGGTTAAATGGACGTTGTTAGGTTCATAGTAATAATACTTATTCTAGAAGGCCGACTTCATTCCTCTACGAGTCTCTACCAAACTTTATTATCTGGTCATATATTAATATCAAAATCTTAAATCTTAATATACATCTTTTGTTCTTTATCACCTTCAATCTTTAAATATATTTTAGCTCATATTAGGACACTTCAATAAGAACTGACTGACTATTCCATCTGAAATACGAGTCTGTTTCATGACGTGAAATGGGACTTTAGCCCCATTTTAATGGCTCCTGCAATTTATAATTAATGAATGAGAAACAATGCTTCAAAAATCTACAGGCATTTCTTAGTTTCACTAGTTTCAAAACCTTCCTACTCTTGCTTACACCTAGACATAATATTTTGAAGGTCTGAAAGTTATAGCAATTGTCTGTCTTAGGTGCTGCTTAGGTGTGTTTTCAAGTTCTTTTTTTTTACTGGGGCTGGGTTCATGGATTCTGAAGCCTTGTTCTTAAAAAATAAATAGAGCCAATCAAGACAATCTGAAAATACTTAACTCACTGTTATTAGTGTTCCCATAATCATATATGTATGTAAATGAAATGATGCCTTACCCATGCAAAATAATCTCGAATACTGTAAATGGTTATCAAGAAGGGTTTGACCCGATCTCCAGTGCAGAGGTTCACAAACCGATTGTGGATCATCGATCAGACCACATTAAGGATCTTGGTTGAAGCTCAAAACTTAGCTCATGATCCAGTGTCACATCCTCCTCGCAATACAAAAAAGAGTTGGAAAAGAAGATAAAAAATATGAAAGATTAGATGGTAAAGATGAAGGCAAATTACAAACAAATGCAAGAAACGAACGTCGGCTTTAACACTCAATTTTCAACATGGGAAGGTAGGTGGTTGGAGCGAGCAGAGGTAGATGAGGGACTTTCAAACACCTAGGTATGTTTTAATATTAAAATTAATTATGTGGTTTCTCGTGGTTATCATGCAATATTGTTTGCCAATTCATTCATGTATAGGAAATTTATAAATTGAGTGTGTTTGTTTATGTGTAGCTATCCTGCAGTTATGATAGTCAATGCATGTTGGGTTTTATTATGAGTAGCCTATTCATGCATGGAAGAAATTTATGCCTTTCAAAATTTTTGGTTCGTTAAACTTCATGTATCATTCGACGTTTGAAAGTTCCTAATTTTATTAGTATTTTTGGTCCAAAACTAAACTTCTAAAGATTGTTCTTGATGGTTTGGACCTTGGGGACAATTTCATACTCATTGACATTTCATTGTTATTCCATAATTTGGTTTAGTAAAAATATTATGTATGATGTACTCGAAGTTTTTCTTCACATTGGCCATTGTATATAATCTTGTGTTTTTTGTCACTGATATAAAATACTTGTGCAATTTTTTTTTCTTGAATACATTGGCTGTCTAATTTAAGTTTTTCTTTGGTCATTCTTCTATATACAACATTGCACGATCATCTAATTATGCAATCTGTTTTTTCAGCATGACAGTAAGTTATAAGGAATATTTTTACATCTAATAGACATGGAATGAAACCTTTCCCTACTAATATGTGCATCATAGGTCATGGAAGCAAATTAGACGACAATTGAAAAGAAAAGAGGCACCCCGATGTGAAAAGGGAATAAAGTAATCAGTTCATGTATAAAAACTTACATAAGAAAAGACTAAATGGAGTTGTTTACTTGCGAGGCATCAAGCTTATTTTCCCATGGAACTCTCATGGAAGTGCTAGATCTTGGCCTACAAGTTGATTAAGTTGATTGATGTTGGGATATACTTGTACGGCTAGTCATGTTCTTTTCTATGGTGTTTATTCATTTTGTTTTGGGGTTGGACTTTTCTTAATGAATTCAAAAAATTGTTTATTCAAGTAATTTCTAATGCAGAAAAAGAATGCTTGGAAAAAGAAATAAATACACATTACTAATAAAGTTACTATCAAACTAGTCCCCATCTTTGGCAGACGGCAAGAAAAGAAACCTAACACGATTTTCTTCAACTTGTATAAGTTTATGCAATTGAAAATAGGGTTGGGGTTAGCAAATCTATGGTTGATCATGAGTTCACGTTTCGAACTCATGTGAGGCATCAAGCTTATTTTCATATCGAACTCATGGAAGTACATAACATTGCTCTTGGCTCACGAAGGTCACCTTTGCCTTGTATAGGTTAGGATTGATTTGAATCTTAGACCATTGGCTGTTATTAGGTGGAACTGAGTTGGATTTTTTGGCTTCAGCTACATTCAGCGAATCAATTATACGTATAAAATATATGTCCCTCTTAGTTCCCGTAGCCTTTAAGACATTTACCTTGTAGCAGTATGAGTAGAATTCAAAAATATTTTTATTGCTTTTATGCACTTTGTATTTGAGAAATTTCTTAAACTCTCAGCAAGAAAGGTTCCTTGGATGTGATTTATTTCAATACCTTAAATTATAGCTTAGCTAGTTTTAATTTATCTTGATTGTTGTATTAGACTATCAAATAATGTATGGTTAAATATTATAGCTTAGCTTAGCTAGTTTTAGTTTTTTAAATTTAAAACTCTCTATAATGCATATGGTTCAACAGTGAAACATGGTATTTTATTTTATGCATTCAAAAGTCCTTTTCAAACTAAAATATTTCAATTAATTATCGACTACTCAGGATCTCTTTGTTTGTCACTTTTTTTAGGTGAACAAACCTTTAAAATAAGATTATTGTTGAAGAACAAATCTTGATTTAAGGAATATTTATGTAATCACATTACGTAAAAGAAGTAGTTAATCTTATATGTATTGTTCTGGAATTAATTAGTTTTCTCCCTATAGAAGGGACGTGTAATCTATGGAAAATTGGCCAACTGCGGAGAGTTAAGAGATGGGATTCAAATGTTAGATGACCCATTGACTCTGTAGTTTCAGCTGAAACATATCAAAACAACCAAAACTTCCATTTTCAAGTTCTCCAAACCTCTCCATAACCAAGAAACTTCAAACCTTACGGGTGCTCCCTCCATACTTGGAATTAGAAGCTTAAAATGGTTTCATTCGAAAGAGGACAACCTCAACTTCATCCCTACATAAAAAAGAGGAAGGAATCGAAAGTAATCAAGGTATATTTTTTGGGTATCCATTCTCAGATTTTTTTTCAAAATTCTTCCTCCATAGACAACCTCATGACTCGAGTTTTGTCTCTTTGGACTCAGGGAGAACAGCTGGACAAGACTCATGAAGAAAGGAAAGCACGAAAATGTGGGGATCATGAAACACCATAAGAGTCATTCTTGGT

mRNA sequence

CATTTTCTCTGCGTCCTTCGGGTAGGTTTGGCGGCCGATCTCAACTCCTCCGCGCTATTTCGGTTCCATGTTATACATTTCACAGCGTCCTAGGAATATTCATCTAAACTCAAAGAGCTGCACATGGATGCTGGTGGATCCTAATGCGAAAAAACGAAGCTTTTAATGACAACAGTTTGGATGTGTTCAAGCGGACGCATTCGTGGAGAGTATTGGAGGAGCAGTTTGGGATGGAGGAATATGTATTCATTTCAAACCTTGCGTTTGCGTTCTTCACAGGAAAACAAGGGACGTGTAATCTATGGAAAATTGGCCAACTGCGGAGAGTTAAGAGATGGGATTCAAATGTTAGATGACCCATTGACTCTGTAGTTTCAGCTGAAACATATCAAAACAACCAAAACTTCCATTTTCAAGTTCTCCAAACCTCTCCATAACCAAGAAACTTCAAACCTTACGGGTGCTCCCTCCATACTTGGAATTAGAAGCTTAAAATGGTTTCATTCGAAAGAGGACAACCTCAACTTCATCCCTACATAAAAAAGAGGAAGGAATCGAAAGTAATCAAGGGAGAACAGCTGGACAAGACTCATGAAGAAAGGAAAGCACGAAAATGTGGGGATCATGAAACACCATAAGAGTCATTCTTGGT

Coding sequence (CDS)

ATGCGAAAAAACGAAGCTTTTAATGACAACAGTTTGGATGTGTTCAAGCGGACGCATTCGTGGAGAGTATTGGAGGAGCAGTTTGGGATGGAGGAATATGTATTCATTTCAAACCTTGCGTTTGCGTTCTTCACAGGAAAACAAGGGACGTGTAATCTATGGAAAATTGGCCAACTGCGGAGAGTTAAGAGATGGGATTCAAATGTTAGATGA

Protein sequence

MRKNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLWKIGQLRRVKRWDSNVR
Homology
[Close]
BLAST of Carg24062 vs. NCBI nr
Match: KAG7023922.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 151.0 bits (380), Expect = 3.9e-33
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0 

Query: 1  MRKNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLWKIGQLR 60
          MRKNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLWKIGQLR
Sbjct: 1  MRKNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLWKIGQLR 60

Query: 61 RVKRWDSNVR 71
          RVKRWDSNVR
Sbjct: 61 RVKRWDSNVR 70

[Close]
BLAST of Carg24062 vs. NCBI nr
Match: AHZ44509.1 (putative progesterone 5 beta-reductase [Thlaspi arvense])

HSP 1 Score: 55.1 bits (131), Expect = 2.9e-04
Identity = 24/53 (45.28%), Postives = 32/53 (60.38%), Query Frame = 0 

Query: 3   KNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLWK 56
           KNEAFN N+ D+FK  H WR+L EQFG+EEY F+           +G   +W+
Sbjct: 270 KNEAFNCNNADIFKWKHLWRILAEQFGIEEYGFVEGKNLGLVEMMKGKERVWE 322

[Close]
BLAST of Carg24062 vs. NCBI nr
Match: AHI88614.1 (putative progesterone 5-beta-reductase [Hesperis matronalis])

HSP 1 Score: 53.5 bits (127), Expect = 8.4e-04
Identity = 28/65 (43.08%), Postives = 36/65 (55.38%), Query Frame = 0 

Query: 3   KNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLW----KIGQ 62
           KNEAFN N+ D+FK  H W+VL EQFG+EEY F            +G   +W    K  Q
Sbjct: 269 KNEAFNCNNADIFKWKHLWKVLAEQFGIEEYGFEEGKNLGLVEMMKGKDRVWEEMVKENQ 328

Query: 63  LRRVK 64
           L+ +K
Sbjct: 329 LQEMK 333

[Close]
BLAST of Carg24062 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.2e-06
Identity = 23/53 (43.40%), Postives = 31/53 (58.49%), Query Frame = 0 

Query: 3   KNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLWK 56
           KNEAFN N+ D+FK  H W++L EQFG+EEY F            +G   +W+
Sbjct: 269 KNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKGKERVWE 321

[Close]
BLAST of Carg24062 vs. ExPASy TrEMBL
Match: A0A024DA00 (Putative progesterone 5 beta-reductase OS=Thlaspi arvense OX=13288 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.4e-04
Identity = 24/53 (45.28%), Postives = 32/53 (60.38%), Query Frame = 0 

Query: 3   KNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLWK 56
           KNEAFN N+ D+FK  H WR+L EQFG+EEY F+           +G   +W+
Sbjct: 270 KNEAFNCNNADIFKWKHLWRILAEQFGIEEYGFVEGKNLGLVEMMKGKERVWE 322

[Close]
BLAST of Carg24062 vs. ExPASy TrEMBL
Match: W6CYX5 (Putative progesterone 5-beta-reductase OS=Hesperis matronalis OX=264418 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.1e-04
Identity = 28/65 (43.08%), Postives = 36/65 (55.38%), Query Frame = 0 

Query: 3   KNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLW----KIGQ 62
           KNEAFN N+ D+FK  H W+VL EQFG+EEY F            +G   +W    K  Q
Sbjct: 269 KNEAFNCNNADIFKWKHLWKVLAEQFGIEEYGFEEGKNLGLVEMMKGKDRVWEEMVKENQ 328

Query: 63  LRRVK 64
           L+ +K
Sbjct: 329 LQEMK 333

[Close]
BLAST of Carg24062 vs. ExPASy TrEMBL
Match: K9Y857 (Putative progesterone 5-beta-reductase OS=Nasturtium officinale OX=65948 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 5.3e-04
Identity = 28/65 (43.08%), Postives = 35/65 (53.85%), Query Frame = 0 

Query: 3   KNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLW----KIGQ 62
           KNEAFN N+ D+FK  H W+VL EQFG+EEY F            +G   +W    K  Q
Sbjct: 269 KNEAFNCNNADIFKWKHLWKVLAEQFGIEEYGFEEGKNLGLVETMKGKERVWEEIVKENQ 328

Query: 63  LRRVK 64
           L+  K
Sbjct: 329 LQETK 333

[Close]
BLAST of Carg24062 vs. ExPASy TrEMBL
Match: A0A024D9Z7 (Putative progesterone 5 beta-reductase OS=Cochlearia officinalis OX=270110 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 9.0e-04
Identity = 22/34 (64.71%), Postives = 27/34 (79.41%), Query Frame = 0 

Query: 3   KNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFI 37
           KNEAFN N+ D+FK  H W+VL EQFG+EEY F+
Sbjct: 269 KNEAFNCNNADIFKWKHMWKVLAEQFGIEEYGFV 302

[Close]
BLAST of Carg24062 vs. ExPASy TrEMBL
Match: H6UF83 (Putative progesterone 5-beta-reductase OS=Lunaria annua OX=153659 PE=4 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 9.0e-04
Identity = 24/53 (45.28%), Postives = 31/53 (58.49%), Query Frame = 0 

Query: 3   KNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLWK 56
           KNEAFN N+ D+FK  H W+VL EQFG+EEY F            +G   +W+
Sbjct: 270 KNEAFNCNNADIFKWKHMWKVLAEQFGIEEYGFEEGKNLGLVQMMKGKERVWE 322

[Close]
BLAST of Carg24062 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 3.0e-07
Identity = 23/53 (43.40%), Postives = 31/53 (58.49%), Query Frame = 0 

Query: 3   KNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLWK 56
           KNEAFN N+ D+FK  H W++L EQFG+EEY F            +G   +W+
Sbjct: 269 KNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKGKERVWE 321

[Close]
BLAST of Carg24062 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 3.0e-07
Identity = 23/53 (43.40%), Postives = 31/53 (58.49%), Query Frame = 0 

Query: 3   KNEAFNDNSLDVFKRTHSWRVLEEQFGMEEYVFISNLAFAFFTGKQGTCNLWK 56
           KNEAFN N+ D+FK  H W++L EQFG+EEY F            +G   +W+
Sbjct: 268 KNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKGKERVWE 320

The following BLAST results are available for this feature:
BLAST of Carg24062 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7023922.13.9e-33100.003-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucurbita argyrosperma subsp. argyros... [more]
AHZ44509.12.9e-0445.28putative progesterone 5 beta-reductase [Thlaspi arvense][more]
AHI88614.18.4e-0443.08putative progesterone 5-beta-reductase [Hesperis matronalis][more]
BLAST of Carg24062 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9STX24.2e-0643.403-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP... [more]
BLAST of Carg24062 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A024DA001.4e-0445.28Putative progesterone 5 beta-reductase OS=Thlaspi arvense OX=13288 PE=2 SV=1[more]
W6CYX54.1e-0443.08Putative progesterone 5-beta-reductase OS=Hesperis matronalis OX=264418 PE=2 SV=... [more]
K9Y8575.3e-0443.08Putative progesterone 5-beta-reductase OS=Nasturtium officinale OX=65948 PE=2 SV... [more]
A0A024D9Z79.0e-0464.71Putative progesterone 5 beta-reductase OS=Cochlearia officinalis OX=270110 PE=2 ... [more]
H6UF839.0e-0445.28Putative progesterone 5-beta-reductase OS=Lunaria annua OX=153659 PE=4 SV=1[more]
BLAST of Carg24062 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G24220.13.0e-0743.40NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24220.23.0e-0743.40NAD(P)-binding Rossmann-fold superfamily protein [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24062-RACarg24062-RAmRNA