Carg24032 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24032
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	4-coumarate-CoA ligase
Location	Carg_Chr02: 2248432 .. 2252571 (+)
RNA-Seq Expression	Carg24032
Synteny	Carg24032

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGTCGAGTATAGCTCAACAGGTTGTTAAAGAATTGGTTGATACAAATTGTTGGGAGGGAGTCCCACGTTCGCTAATTTAGGGAATGATCATGAGCTTATGAGTAAAGAATATATCTCCATTGGTACGATGTTTTTTGGAAAAGCCCGAAACAAAGGCACAACGACTTATGCTCGTAGAGAGTCGTTTTCATTAAATGACATAGGAAAATGAGAATTTAGCCTTATTCATTAAATGGCATACGGAAACAAGAATCTTGTCCCGCCCGTCCTGTTTCTTTCAATGAAAACCCTTGGTAAACGAGAATCCAATCCCACGCCTGCCTGTTCCTTTCGATGAAAATTTTTGTAGGATAAAATAGGAGGCGGGATGAGATGGGATGGGAAATCCCGTCCCGCCCTATTCCTTTGGACATCTCTAATAACTACGAATATTAAAATAACATGCAGGTAGATGGGGAGAATCCGAATATATACATAGGAGGTGGGATGGGAAATCTGGTCCCGTTCCTTTGGACATCTCTAATAACTATAAATAATGAAATAGTGTTTGTTAACGTGCAGGTAGATGGAGAGAATCCGAACATATACTTGGGAACGAACGACGTGGTTTTATGTGTGCTTCCGATGTTCCACATATTCTCATTGAGTAGCATAGTTTTGATTTCGATTCGATCGGGAGCAACAATATTATTGATAGAGAAGTTCGAGATTGAGACGTCAATGAGCCTGATAGAGAGGCATGGGGTGACGGTGGCAACCGTGGTGCCGCCGATGGTGTTGATGATGGTGAAGAATCCAAAGGTGGCGGATTTCAACCTAAGCTCGATCAGAATGGTGATTTCAGGGGCGGCGCCGCTAGGAAAGCAGATAGAGGAGGCCCTCATGGAAAGGATCCCTCAAGCAGTTATTGGTCAGGTATGGACTATAATATAATGTTATATAATATGGTATTAATGTTATTCGAATGAACTATGTTTACGTTGATATAATTATAGGGTTACGGGATGACGGAAGCAGGTCCGGTGTTGAGTATGTGCACGGCTTTTGCGAAAGAGCCTCCGATGCCAACAAAAATAGGGTCGTGTGGGAGAGTTGTGAGAAACTCAGAGCTCAAAATCATTGACCCAAAAACCGGTGTCTCCCTTACCTATAATCAACCCGGAGAGATCTGTATTCGCGGCCCACAAATTATGAAAGGTTCTCTTTTTCTCGTTTTTTTCGTTTTTAAATATTTTTAGAGTATATAAAATGTCGATATTCTTTCTCTTTTGTTCGAAACTTTTAAAGGGTTTTCAAAAAACTTAATGATCGAAAAAATTGATCAACCTAAACAGTTTGAATTTGGTTACGAATTTATTAAATTGGGTTCGAATAAATTATAATTTTTTATTTATGATTTAATTATACTTATATATATTTATTGATTTTATTTAACTTCATAATTTATTATTATTATTTGTTCTTAATTTTTTTAAAAAAATTTAAATATTTGAATATAAATAAAAAATATTTTACCAACTAATATTTTACTTTTATTAAAAACAAAAATTTGAATTAAAGTAAATACTATTTTGCTACCACTGACACGTGACAAAAATATTTATAAAATGCTAAAATAAATGGAATTAAAGTTTGGTGCTAAAATGACATGGCTGTGATGGTAGGATATTTGAACGACCCAGTGGCCACGTCATTGACAGTCGACGTGGAGGGGTGGCTTCACACGGGTGACATTGGATACATTGACGAAGAAGAAGAAATATTTATTGTTGACCGGATCAAGGAGATTATCAAATATAAGGGTTTCCAGGTACACGTCACTATTATCATAATTTCTACCCAAATAATAATAATAATAATAAATTATTATTAATAATAAATTAATAATAATAATAATAAATTATTTTCGCTATTATATTTAATTCTTCACTAATAATGGGATTATTGAGCTTTAATGATTAATTCAAAGCAATAATTAATATAAAAACGATTAAATTAGCTAAAGTCAATTTAACATAATTAGCATGTTTCTGAAATAAAGTTTGAATCTCTAATTTTTTATTATTGTAATTAACTTTGTAAATTATGGTATTTACAGGTAGCACCGGTCGAGTTAGAATCTCTTCTTCTAACTCATCCATCTATTATGGAGGTCGCTGTGGTTGCGTAAGTGCCCGATTAAAATTCTTTAATATTTAATTCTTAAAAGAAAAAAAAATTAAAAATTAAAATATTGACTAAATTAACAAAAATATATATATATATATTGGAAAAATATCAACCATAAATCCCTAAATTTTAAATAAAATCTAAAAAAAATATAAAAAAAAAAATTTATAGTCTCTAATATTTTATTTTTTAAAAAATATATATATATAAATATATCCTTATAAATTTTTTAAAATTTTGTTTTAAATTATGAAAAAAAAATAAAGAAATTGGAGAAAATTACAGGGAAAATGATGAAATTGCTGGGGAAGTTCCAGTGGCCTTCGTAGTTTGTTCAGCTGGTAATCAACTTACCCAGGAAGAAGTAAAAGAATTCATTGCAAAGCAGGTTTGTATTATAAAAATGCTATTTTCCCCATATAATAACATTATAATTTTGATAATACTTCGAGTTTATGCTTCGGTTTCTAATTTTACAAGTATTAACGAGCATCGGGCTAAAATCGTCTTTTCATACGTAAAAAGAAGATATATAATATTTAAAAGTTTAGGAATTAAATTAAATTTTAAAATTAATTTATTCTCACTTTCCTTTTATTTTCTAATGCAAAATCATCAGGTTGTGTTCTACAAGAGATTGCACCGAGTTTATTTTGTACCCACGATTCCAAAATTGCCCTCTGGAAAGATCTTGAAAAAAGATCTCAAAGCCAAGCTCTCGAACTCACCTAAAGCTACAAAAATGAAAGAGATAGGGAGAGAGATTAAGTAACATGGTCGGATCGTGTGTGGTAGAAAATAAAAAGGAATGTAAGGTGAATTTAGATTTCAAATCTCGAACTTGAACCGTAACGTTTTTATTTTATATTTCAATTATGTGACTAATAGATATAAATATTTTTAATAATAAGTCGTTGAGTTTTTTTATTTTTTATTTTTTTTTTTAATTTAAGAATATTAATAAAAAAAAAATTAAAAATAGATATAATATCAAATTTTTTAATCCAAACCTAAAACCAACATCAAATTATAGAATTAACTTTATTTAGTCACAATATTACTTTTCATATTCTTTAAACATTTTAAACCGCGTTTAAATTATATTCATAAAATACAGGATAAAAAATCGATATAACAATAATCGGGAAGATTATCTATATTAATTATAAAAAAAAATGATATATCTCGTCTAATATCCTCTTATTTTTTTATATAATTTTATGGACTAAATTAATAAAAATATTCATAAATTTTATACTTTTGTATAAAAAAATATCTAAGAATTTAGAGATCTATGAAATATAAATAGATATTTTGATAAAAACTTTTCTTTTTAGATTAATATTACTTTTAAAAAATTAAAAACATAATTATTATTATTATTATTTTAATTAAAAGGTATAAAGGAGAGTAAATACATTTTAAAGTTTTTTTTTTATTTTTTTTAAAGTTATTATTGAAAATTTAAAAATTAGATATTTTGAGATAAAATACAAAAGTTTTAGGGTATTTAGTGTAACCTATAAAAAGTTGGATCCAAATTTTATAGTTTGATCTCCGTCGAAACGTTGCTCTCCTTGGCGCTCTCTCTTTTGTGTGTTTCAGGCTGCGATTGGTCGGTGTATGAATGGCGTCGAGTCTCTTCTTCCGTCTTCGGCAGGGAATATGAGTATTGTTAGCAGCCGAGTCTCTTCTTCCGTCTTCGGCAGGGAATATGAGTATTGTTAGCAGCCGAGTCTCTTCTTCCGTCTTCGGCAGGGAATATGAGTATTGTTACAGCCGGAGTCTCTTTCTTCCGTCTTCGAGTATTGTTAAGCAGCGAGTCTCTTTCTTCCGTCTTCGCAGGGAATATGAGTATTCTCTTCTTCCGTTAGTATTGCCCGAAGTCTCTTCTTCCGTCTTCGGCAGGGAATATGAGTATTGTTAGCAGCCGAGTCTCTTCTTCCGTCTTCGGCAGGGAATATGAGTAT

mRNA sequence

GTGTCGAGTATAGCTCAACAGGTTGTTAAAGAATTGGTTGATACAAATTGTTGGGAGGGAGTCCCACGTTCGCTAATTTAGGGAATGATCATGAGCTTATGAGTAAAGAATATATCTCCATTGGTACGATGTTTTTTGGAAAAGCCCGAAACAAAGGCACAACGACTTATGCTCGTAGAGAGTCGTTTTCATTAAATGACATAGGAAAATGAGAATTTAGCCTTATTCATTAAATGGCATACGGAAACAAGAATCTTGTCCCGCCCGTCCTGTTTCTTTCAATGAAAACCCTTGGTAAACGAGAATCCAATCCCACGCCTGCCTGTTCCTTTCGATGAAAATTTTTGTAGGATAAAATAGGAGGCGGGATGAGATGGGATGGGAAATCCCGTCCCGCCCTATTCCTTTGGACATCTCTAATAACTACGAATATTAAAATAACATGCAGGTAGATGGGGAGAATCCGAATATATACATAGGAGGTGGGATGGGAAATCTGGTCCCGTTCCTTTGGACATCTCTAATAACTATAAATAATGAAATAGTGTTTGTTAACGTGCAGGTAGATGGAGAGAATCCGAACATATACTTGGGAACGAACGACGTGGTTTTATGTGTGCTTCCGATGTTCCACATATTCTCATTGAGTAGCATAGTTTTGATTTCGATTCGATCGGGAGCAACAATATTATTGATAGAGAAGTTCGAGATTGAGACGTCAATGAGCCTGATAGAGAGGCATGGGGTGACGGTGGCAACCGTGGTGCCGCCGATGGTGTTGATGATGGTGAAGAATCCAAAGGTGGCGGATTTCAACCTAAGCTCGATCAGAATGGTGATTTCAGGGGCGGCGCCGCTAGGAAAGCAGATAGAGGAGGCCCTCATGGAAAGGATCCCTCAAGCAGTTATTGGTCAGGTAGCACCGGTCGAGTTAGAATCTCTTCTTCTAACTCATCCATCTATTATGGAGGTCGCTGTGGTTGCGGAAAATGATGAAATTGCTGGGGAAGTTCCAGTGGCCTTCGTAGTTTGTTCAGCTGGTAATCAACTTACCCAGGAAGAAGTAAAAGAATTCATTGCAAAGCAGGCTGCGATTGGTCGGTGTATGAATGGCGTCGAGTCTCTTCTTCCGTCTTCGGCAGGGAATATGAGTATTGTTAGCAGCCGAGTCTCTTCTTCCGTCTTCGGCAGGGAATATGAGTATTGGAATATGAGTATTGTTAGCAGCCGAGTCTCTTCTTCCGTCTTCGGCAGGGAATATGAGTAT

Coding sequence (CDS)

ATGCAGGTAGATGGGGAGAATCCGAATATATACATAGGAGGTGGGATGGGAAATCTGGTCCCGTTCCTTTGGACATCTCTAATAACTATAAATAATGAAATAGTGTTTGTTAACGTGCAGGTAGATGGAGAGAATCCGAACATATACTTGGGAACGAACGACGTGGTTTTATGTGTGCTTCCGATGTTCCACATATTCTCATTGAGTAGCATAGTTTTGATTTCGATTCGATCGGGAGCAACAATATTATTGATAGAGAAGTTCGAGATTGAGACGTCAATGAGCCTGATAGAGAGGCATGGGGTGACGGTGGCAACCGTGGTGCCGCCGATGGTGTTGATGATGGTGAAGAATCCAAAGGTGGCGGATTTCAACCTAAGCTCGATCAGAATGGTGATTTCAGGGGCGGCGCCGCTAGGAAAGCAGATAGAGGAGGCCCTCATGGAAAGGATCCCTCAAGCAGTTATTGGTCAGGTAGCACCGGTCGAGTTAGAATCTCTTCTTCTAACTCATCCATCTATTATGGAGGTCGCTGTGGTTGCGGAAAATGATGAAATTGCTGGGGAAGTTCCAGTGGCCTTCGTAGTTTGTTCAGCTGGTAATCAACTTACCCAGGAAGAAGTAAAAGAATTCATTGCAAAGCAGGCTGCGATTGGTCGGTGTATGAATGGCGTCGAGTCTCTTCTTCCGTCTTCGGCAGGGAATATGAGTATTGTTAGCAGCCGAGTCTCTTCTTCCGTCTTCGGCAGGGAATATGAGTATTGGAATATGAGTATTGTTAGCAGCCGAGTCTCTTCTTCCGTCTTCGGCAGGGAATATGAGTAT

Protein sequence

MQVDGENPNIYIGGGMGNLVPFLWTSLITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIEKFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEALMERIPQAVIGQVAPVELESLLLTHPSIMEVAVVAENDEIAGEVPVAFVVCSAGNQLTQEEVKEFIAKQAAIGRCMNGVESLLPSSAGNMSIVSSRVSSSVFGREYEYWNMSIVSSRVSSSVFGREYEY

Homology

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BLAST of Carg24032 vs. NCBI nr
Match: KAG7035152.1 (hypothetical protein SDJN02_01947, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 520.0 bits (1338), Expect = 1.2e-143
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MQVDGENPNIYIGGGMGNLVPFLWTSLITINNEIVFVNVQVDGENPNIYLGTNDVVLCVL 60
           MQVDGENPNIYIGGGMGNLVPFLWTSLITINNEIVFVNVQVDGENPNIYLGTNDVVLCVL
Sbjct: 1   MQVDGENPNIYIGGGMGNLVPFLWTSLITINNEIVFVNVQVDGENPNIYLGTNDVVLCVL 60

Query: 61  PMFHIFSLSSIVLISIRSGATILLIEKFEIETSMSLIERHGVTVATVVPPMVLMMVKNPK 120
           PMFHIFSLSSIVLISIRSGATILLIEKFEIETSMSLIERHGVTVATVVPPMVLMMVKNPK
Sbjct: 61  PMFHIFSLSSIVLISIRSGATILLIEKFEIETSMSLIERHGVTVATVVPPMVLMMVKNPK 120

Query: 121 VADFNLSSIRMVISGAAPLGKQIEEALMERIPQAVIGQVAPVELESLLLTHPSIMEVAVV 180
           VADFNLSSIRMVISGAAPLGKQIEEALMERIPQAVIGQVAPVELESLLLTHPSIMEVAVV
Sbjct: 121 VADFNLSSIRMVISGAAPLGKQIEEALMERIPQAVIGQVAPVELESLLLTHPSIMEVAVV 180

Query: 181 AENDEIAGEVPVAFVVCSAGNQLTQEEVKEFIAKQAAIGRCMNGVESLLPSSAGNMSIVS 240
           AENDEIAGEVPVAFVVCSAGNQLTQEEVKEFIAKQAAIGRCMNGVESLLPSSAGNMSIVS
Sbjct: 181 AENDEIAGEVPVAFVVCSAGNQLTQEEVKEFIAKQAAIGRCMNGVESLLPSSAGNMSIVS 240

Query: 241 SRVSSSVFGREYEYWNMSIVSSRVSSSVFGREYEY 276
           SRVSSSVFGREYEYWNMSIVSSRVSSSVFGREYEY
Sbjct: 241 SRVSSSVFGREYEYWNMSIVSSRVSSSVFGREYEY 275

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BLAST of Carg24032 vs. NCBI nr
Match: XP_022947959.1 (4-coumarate--CoA ligase 3 [Cucurbita moschata])

HSP 1 Score: 277.7 bits (709), Expect = 1.1e-70
Identity = 178/315 (56.51%), Postives = 186/315 (59.05%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  + +  +  QVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE
Sbjct: 210 VLTHKSMVSSIAQQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 269

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA
Sbjct: 270 KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 329

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           LMERIPQAVIG                                                 
Sbjct: 330 LMERIPQAVIGQGYGMTEAGPVLSMCTAFAKEPPMPTKIGSCGRVVRNSELKIIDPKTGV 389

Query: 207 ------------------------------------------------------------ 226
                                                                       
Sbjct: 390 SLTYNQPGEICIRGPQIMKGYLNDPVATSLTVDVEGWLHTGDIGYIDEEEEIFIVDRIKE 449

[Close]

BLAST of Carg24032 vs. NCBI nr
Match: KAG6605161.1 (4-coumarate--CoA ligase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 277.7 bits (709), Expect = 1.1e-70
Identity = 178/315 (56.51%), Postives = 186/315 (59.05%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  + +  +  QVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE
Sbjct: 210 VLTHKSMVSSIAQQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 269

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA
Sbjct: 270 KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 329

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           LMERIPQAVIG                                                 
Sbjct: 330 LMERIPQAVIGQGYGMTEAGPVLSMCTAFAKEPPMPTKIGSCGRVVRNSELKIIDPKTGV 389

Query: 207 ------------------------------------------------------------ 226
                                                                       
Sbjct: 390 SLTYNQPGEICIRGPQIMKGYLNDPVATSLTVDVEGWLHTGDIGYIDEEEEIFIVDRIKE 449

[Close]

BLAST of Carg24032 vs. NCBI nr
Match: XP_023532645.1 (4-coumarate--CoA ligase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 266.2 bits (679), Expect = 3.2e-67
Identity = 170/315 (53.97%), Postives = 184/315 (58.41%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  N +  +  QVDG+NPNIYLG NDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE
Sbjct: 210 VLTHKNMVSSIAQQVDGQNPNIYLGRNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 269

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEIETSMSLIE+HGVTVATVVPPMV+MMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA
Sbjct: 270 KFEIETSMSLIEKHGVTVATVVPPMVVMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 329

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           LMERIPQA+IG                                                 
Sbjct: 330 LMERIPQAIIGQGYGMTEAGPVLSMCTAFAKDPPMPTKIGSCGRVVRNSELKIIDPKTGV 389

Query: 207 ------------------------------------------------------------ 226
                                                                       
Sbjct: 390 SLTYNQLGEICIRGPQIMKGYLNDPVATSLTVDVEGWLHTGDIGFIDEEEEIFIVDRIKE 449

[Close]

BLAST of Carg24032 vs. NCBI nr
Match: XP_038901547.1 (4-coumarate--CoA ligase 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 218.4 bits (555), Expect = 7.7e-53
Identity = 134/293 (45.73%), Postives = 166/293 (56.66%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  N +  V  QVDGENPN+YL  ND+VLCVLPMFHIFSLSSIVL+SIRSGA +LL+E
Sbjct: 219 ILTHKNMVSSVAQQVDGENPNLYLRRNDIVLCVLPMFHIFSLSSIVLVSIRSGAALLLME 278

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEIE+ + LIE+HGVTVATVVPP+V+ +VKNP+ ADF+LSSIRMV+SGAAPL K++EEA
Sbjct: 279 KFEIESLLRLIEKHGVTVATVVPPLVVALVKNPRAADFDLSSIRMVLSGAAPLRKELEEA 338

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           LM+R+PQA+ G                                                 
Sbjct: 339 LMQRLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSDLKVIDPQTGA 398

Query: 207 -------------------------------------------------------QVAPV 216
                                                                  QVAP 
Sbjct: 399 SLSYNQSGEICLRGPQVMKVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPA 458

[Close]

BLAST of Carg24032 vs. ExPASy Swiss-Prot
Match: P31687 (4-coumarate--CoA ligase 2 OS=Glycine max OX=3847 PE=2 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 1.8e-44
Identity = 121/314 (38.54%), Postives = 157/314 (50.00%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  +    V  QVDGENPN+YL T DV+LCVLP+FHIFSL+S++L ++R+G+ +LL++
Sbjct: 218 ILTHKSLTTSVAQQVDGENPNLYLTTEDVLLCVLPLFHIFSLNSVLLCALRAGSAVLLMQ 277

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEI T + LI+RH V+VA VVPP+VL + KNP VADF+LSSIR+V+SGAAPLGK++EEA
Sbjct: 278 KFEIGTLLELIQRHRVSVAMVVPPLVLALAKNPMVADFDLSSIRLVLSGAAPLGKELEEA 337

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           L  R+PQAV+G                                                 
Sbjct: 338 LRNRMPQAVLGQGYGMTEAGPVLSMCLGFAKQPFQTKSGSCGTVVRNAELKVVDPETGRS 397

Query: 207 ------------------------------------------------------------ 226
                                                                       
Sbjct: 398 LGYNQPGEICIRGQQIMKGYLNDEAATASTIDSEGWLHTGDVGYVDDDDEIFIVDRVKEL 457

[Close]

BLAST of Carg24032 vs. ExPASy Swiss-Prot
Match: Q9S777 (4-coumarate--CoA ligase 3 OS=Arabidopsis thaliana OX=3702 GN=4CL3 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.8e-44
Identity = 121/347 (34.87%), Postives = 170/347 (48.99%), Query Frame = 0


Query: 4   DGENP---NIYIGGGMGNLVPFLWTS-------LITINNEIVFVNVQVDGENPNIYLGTN 63
           D  NP    + IGG     +PF   +       ++T  + I  V  QVDG+NPN+YL +N
Sbjct: 191 DETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSN 250

Query: 64  DVVLCVLPMFHIFSLSSIVLISIRSGATILLIEKFEIETSMSLIERHGVTVATVVPPMVL 123
           DV+LCVLP+FHI+SL+S++L S+RSGAT+LL+ KFEI   + LI+RH VT+A +VPP+V+
Sbjct: 251 DVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVI 310

Query: 124 MMVKNPKVADFNLSSIRMVISGAAPLGKQIEEALMERIPQAVIG---------------- 183
            + KNP V  ++LSS+R V+SGAAPLGK+++++L  R+PQA++G                
Sbjct: 311 ALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSL 370

Query: 184 ------------------------------------------------------------ 226
                                                                       
Sbjct: 371 GFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEAT 430

[Close]

BLAST of Carg24032 vs. ExPASy Swiss-Prot
Match: Q42982 (4-coumarate--CoA ligase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CL2 PE=1 SV=2)

HSP 1 Score: 163.7 bits (413), Expect = 3.0e-39
Identity = 111/324 (34.26%), Postives = 156/324 (48.15%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  + +  V  QVDGENPN+++G  DV LCVLP+FHIFSL+S++L ++R+GA + L+ 
Sbjct: 230 VLTHRSVVSGVAQQVDGENPNLHMGAGDVALCVLPLFHIFSLNSVLLCAVRAGAAVALMP 289

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           +FE+   +  IER  VTVA VVPP+VL + KNP V   +LSSIR+V+SGAAPLGK++E+A
Sbjct: 290 RFEMGAMLGAIERWRVTVAAVVPPLVLALAKNPFVERHDLSSIRIVLSGAAPLGKELEDA 349

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           L  R+PQA+ G                                                 
Sbjct: 350 LRARLPQAIFGQGYGMTEAGPVLSMCPAFAKEPTPAKSGSCGTVVRNAELKVVDPDTGFS 409

Query: 207 ------------------------------------------------------------ 234
                                                                       
Sbjct: 410 LGRNLPGEICIRGPQIMKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKEL 469

[Close]

BLAST of Carg24032 vs. ExPASy Swiss-Prot
Match: Q6ETN3 (4-coumarate--CoA ligase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=4CL3 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 4.7e-37
Identity = 96/314 (30.57%), Postives = 154/314 (49.04%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  + I  V  QVDGENPN+Y   +DV+LC+LP+FHI+SL+S++L  +R+G+TI+++ 
Sbjct: 199 MLTHRSLITSVAQQVDGENPNLYFSKDDVILCLLPLFHIYSLNSVLLAGLRAGSTIVIMR 258

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KF++   + L+ +H +T+A  VPP+V+ + K+P+V   +L+SIRMV+SGAAP+GK +++A
Sbjct: 259 KFDLGALVDLVRKHNITIAPFVPPIVVEIAKSPRVTAEDLASIRMVMSGAAPMGKDLQDA 318

Query: 147 LMERIPQAVIG------------------------------------------------- 206
            M +IP AV+G                                                 
Sbjct: 319 FMAKIPNAVLGQGYGMTEAGPVLAMCLAFAKEPFKVKSGSCGTVVRNAELKIVDPDTGTS 378

Query: 207 ------------------------------------------------------------ 226
                                                                       
Sbjct: 379 LGRNQSGEICIRGEQIMKGYLNDPEATKNTIDEDGWLHTGDIGFVDDDDEIFIVDRLKEI 438

[Close]

BLAST of Carg24032 vs. ExPASy Swiss-Prot
Match: Q9S725 (4-coumarate--CoA ligase 2 OS=Arabidopsis thaliana OX=3702 GN=4CL2 PE=1 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 1.1e-36
Identity = 107/314 (34.08%), Postives = 149/314 (47.45%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T    +  V  QVDGENPN+Y   +DV+LCVLPMFHI++L+SI+L S+R GATIL++ 
Sbjct: 214 MLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIMLCSLRVGATILIMP 273

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEI   +  I+R  VTVA VVPP+VL + K+P+   ++LSS+RMV SGAAPLGK++E+A
Sbjct: 274 KFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRMVKSGAAPLGKELEDA 333

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           +  + P A +G                                                 
Sbjct: 334 ISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDS 393

Query: 207 ------------------------------------------------------------ 226
                                                                       
Sbjct: 394 LPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKEL 453

[Close]

BLAST of Carg24032 vs. ExPASy TrEMBL
Match: A0A6J1G8F9 (4-coumarate--CoA ligase 3 OS=Cucurbita moschata OX=3662 GN=LOC111451685 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 5.2e-71
Identity = 178/315 (56.51%), Postives = 186/315 (59.05%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  + +  +  QVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE
Sbjct: 210 VLTHKSMVSSIAQQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 269

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA
Sbjct: 270 KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 329

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           LMERIPQAVIG                                                 
Sbjct: 330 LMERIPQAVIGQGYGMTEAGPVLSMCTAFAKEPPMPTKIGSCGRVVRNSELKIIDPKTGV 389

Query: 207 ------------------------------------------------------------ 226
                                                                       
Sbjct: 390 SLTYNQPGEICIRGPQIMKGYLNDPVATSLTVDVEGWLHTGDIGYIDEEEEIFIVDRIKE 449

[Close]

BLAST of Carg24032 vs. ExPASy TrEMBL
Match: A0A5A7THZ8 (4-coumarate--CoA ligase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G002030 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.2e-51
Identity = 138/315 (43.81%), Postives = 168/315 (53.33%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  N +  V  QVDGENPN+YL  ND+VLCVLPMFHIFSLSSIVLISIRSGA +LL+E
Sbjct: 220 ILTHKNMVSSVAQQVDGENPNLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLME 279

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEIE+ + L+ERH VTVATVVPP+V+ +VKNPKVADFNLSSIR+V+SGAAPL K++EEA
Sbjct: 280 KFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEA 339

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           LMER+PQA+ G                                                 
Sbjct: 340 LMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGA 399

Query: 207 ------------------------------------------------------------ 226
                                                                       
Sbjct: 400 SLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKE 459

[Close]

BLAST of Carg24032 vs. ExPASy TrEMBL
Match: A0A1S3CJB3 (4-coumarate--CoA ligase 2 OS=Cucumis melo OX=3656 GN=LOC103501067 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.2e-51
Identity = 138/315 (43.81%), Postives = 168/315 (53.33%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  N +  V  QVDGENPN+YL  ND+VLCVLPMFHIFSLSSIVLISIRSGA +LL+E
Sbjct: 220 ILTHKNMVSSVAQQVDGENPNLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLME 279

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEIE+ + L+ERH VTVATVVPP+V+ +VKNPKVADFNLSSIR+V+SGAAPL K++EEA
Sbjct: 280 KFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEA 339

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           LMER+PQA+ G                                                 
Sbjct: 340 LMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGA 399

Query: 207 ------------------------------------------------------------ 226
                                                                       
Sbjct: 400 SLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKE 459

[Close]

BLAST of Carg24032 vs. ExPASy TrEMBL
Match: A0A1U8B782 (4-coumarate--CoA ligase 2-like OS=Nelumbo nucifera OX=4432 GN=LOC104611494 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 8.9e-47
Identity = 115/241 (47.72%), Postives = 154/241 (63.90%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  + I  V  QVDGENPN++L T DV LCVLP+FHIFSL+S++L S+R+G  +LL++
Sbjct: 237 ILTHKSLISSVAQQVDGENPNLHLKTEDVFLCVLPLFHIFSLNSVLLCSLRAGTGVLLMQ 296

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEI   + LI+R+ V+VA VVPP+VL + KN  V +F+LSSIR+++SGAAPLGK++EEA
Sbjct: 297 KFEIGALLDLIQRYRVSVAAVVPPLVLALAKNQMVENFDLSSIRIILSGAAPLGKELEEA 356

Query: 147 LMERIPQAVIG------------------------------------------QVAPVEL 206
           L  ++PQAV G                                          QV P EL
Sbjct: 357 LHSKVPQAVFGQGYGMTEAGPVLSMCPGFAKQPFPTKSGSCGTVVRNAEYKGFQVPPAEL 416

Query: 207 ESLLLTHPSIMEVAVVAENDEIAGEVPVAFVVCSAGNQLTQEEVKEFIAKQAAIGRCMNG 226
           E+LL++HPSI + AVV + D +AGEVPVAFVV S G  LT+E VKEFIAKQ    + ++ 
Sbjct: 417 EALLVSHPSIADAAVVPQKDAVAGEVPVAFVVRSNGFDLTEESVKEFIAKQVVFYKKLHK 476

[Close]

BLAST of Carg24032 vs. ExPASy TrEMBL
Match: A0A6J1DCS3 (4-coumarate--CoA ligase 2-like OS=Momordica charantia OX=3673 GN=LOC111018921 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 1.5e-46
Identity = 130/304 (42.76%), Postives = 158/304 (51.97%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T  + +  V  QVDGENPNIYL T+DVVLCVLPMFHIFSLSS++L SIR GA ILL+E
Sbjct: 100 VLTHKSMVSSVAQQVDGENPNIYLRTDDVVLCVLPMFHIFSLSSVILSSIRCGAAILLME 159

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEIE  + LIERH VTVA VVPP+VL + KN KVA+F+LSSIRMV SGAAPL K++EEA
Sbjct: 160 KFEIEALLRLIERHRVTVAAVVPPLVLALAKNSKVAEFDLSSIRMVHSGAAPLRKELEEA 219

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           LM+++PQA  G                                                 
Sbjct: 220 LMQKMPQATFGQGYGMTEAGPVVSMCSAFAKEPMATKSGSCGRVVRNAELKVIDPQTGSS 279

Query: 207 ------------------------------------------------------------ 216
                                                                       
Sbjct: 280 LGYNQPGEICIRGPQIMKGYLNNPEATSLTIDMEGWLHTGDIGYVDDDEEIFIVDRVKEI 339

[Close]

BLAST of Carg24032 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 181.0 bits (458), Expect = 1.3e-45
Identity = 121/347 (34.87%), Postives = 170/347 (48.99%), Query Frame = 0


Query: 4   DGENP---NIYIGGGMGNLVPFLWTS-------LITINNEIVFVNVQVDGENPNIYLGTN 63
           D  NP    + IGG     +PF   +       ++T  + I  V  QVDG+NPN+YL +N
Sbjct: 191 DETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSN 250

Query: 64  DVVLCVLPMFHIFSLSSIVLISIRSGATILLIEKFEIETSMSLIERHGVTVATVVPPMVL 123
           DV+LCVLP+FHI+SL+S++L S+RSGAT+LL+ KFEI   + LI+RH VT+A +VPP+V+
Sbjct: 251 DVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVI 310

Query: 124 MMVKNPKVADFNLSSIRMVISGAAPLGKQIEEALMERIPQAVIG---------------- 183
            + KNP V  ++LSS+R V+SGAAPLGK+++++L  R+PQA++G                
Sbjct: 311 ALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSL 370

Query: 184 ------------------------------------------------------------ 226
                                                                       
Sbjct: 371 GFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEAT 430

[Close]

BLAST of Carg24032 vs. TAIR 10
Match: AT1G65060.2 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 157.1 bits (396), Expect = 2.0e-38
Identity = 82/165 (49.70%), Postives = 119/165 (72.12%), Query Frame = 0


Query: 4   DGENP---NIYIGGGMGNLVPFLWTS-------LITINNEIVFVNVQVDGENPNIYLGTN 63
           D  NP    + IGG     +PF   +       ++T  + I  V  QVDG+NPN+YL +N
Sbjct: 191 DETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSN 250

Query: 64  DVVLCVLPMFHIFSLSSIVLISIRSGATILLIEKFEIETSMSLIERHGVTVATVVPPMVL 123
           DV+LCVLP+FHI+SL+S++L S+RSGAT+LL+ KFEI   + LI+RH VT+A +VPP+V+
Sbjct: 251 DVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVI 310

Query: 124 MMVKNPKVADFNLSSIRMVISGAAPLGKQIEEALMERIPQAVIGQ 159
            + KNP V  ++LSS+R V+SGAAPLGK+++++L  R+PQA++GQ
Sbjct: 311 ALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQ 355

[Close]

BLAST of Carg24032 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 155.2 bits (391), Expect = 7.5e-38
Identity = 107/314 (34.08%), Postives = 149/314 (47.45%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T    +  V  QVDGENPN+Y   +DV+LCVLPMFHI++L+SI+L S+R GATIL++ 
Sbjct: 214 MLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIMLCSLRVGATILIMP 273

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEI   +  I+R  VTVA VVPP+VL + K+P+   ++LSS+RMV SGAAPLGK++E+A
Sbjct: 274 KFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRMVKSGAAPLGKELEDA 333

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           +  + P A +G                                                 
Sbjct: 334 ISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDS 393

Query: 207 ------------------------------------------------------------ 226
                                                                       
Sbjct: 394 LPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKEL 453

[Close]

BLAST of Carg24032 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 148.7 bits (374), Expect = 7.0e-36
Identity = 107/328 (32.62%), Postives = 156/328 (47.56%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T    +  V  QVDGENPN+Y  ++DV+LCVLPMFHI++L+SI+L  +R GA IL++ 
Sbjct: 221 MLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALNSIMLCGLRVGAAILIMP 280

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEI   + LI+R  VTVA +VPP+VL + K+ +   ++LSSIR+V SGAAPLGK++E+A
Sbjct: 281 KFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSIRVVKSGAAPLGKELEDA 340

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           +  + P A +G                                                 
Sbjct: 341 VNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDS 400

Query: 207 ------------------------------------------------------------ 237
                                                                       
Sbjct: 401 LSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKEL 460

[Close]

BLAST of Carg24032 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 147.5 bits (371), Expect = 1.6e-35
Identity = 102/304 (33.55%), Postives = 145/304 (47.70%), Query Frame = 0


Query: 27  LITINNEIVFVNVQVDGENPNIYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIE 86
           ++T    +  V  QVDGENPN+Y  ++DV+LCVLPMFHI++L+SI+L  +R GA IL++ 
Sbjct: 221 MLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALNSIMLCGLRVGAAILIMP 280

Query: 87  KFEIETSMSLIERHGVTVATVVPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEA 146
           KFEI   + LI+R  VTVA +VPP+VL + K+ +   ++LSSIR+V SGAAPLGK++E+A
Sbjct: 281 KFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSIRVVKSGAAPLGKELEDA 340

Query: 147 LMERIPQAVIG------------------------------------------------- 206
           +  + P A +G                                                 
Sbjct: 341 VNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDS 400

Query: 207 ------------------------------------------------------------ 216
                                                                       
Sbjct: 401 LSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKEL 460

The following BLAST results are available for this feature:

BLAST of Carg24032 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035152.1	1.2e-143	100.00	hypothetical protein SDJN02_01947, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022947959.1	1.1e-70	56.51	4-coumarate--CoA ligase 3 [Cucurbita moschata]	[more]
KAG6605161.1	1.1e-70	56.51	4-coumarate--CoA ligase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023532645.1	3.2e-67	53.97	4-coumarate--CoA ligase 3 [Cucurbita pepo subsp. pepo]	[more]
XP_038901547.1	7.7e-53	45.73	4-coumarate--CoA ligase 3 isoform X2 [Benincasa hispida]	[more]

BLAST of Carg24032 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P31687	1.8e-44	38.54	4-coumarate--CoA ligase 2 OS=Glycine max OX=3847 PE=2 SV=2	[more]
Q9S777	1.8e-44	34.87	4-coumarate--CoA ligase 3 OS=Arabidopsis thaliana OX=3702 GN=4CL3 PE=1 SV=1	[more]
Q42982	3.0e-39	34.26	4-coumarate--CoA ligase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CL2 PE=1 ...	[more]
Q6ETN3	4.7e-37	30.57	4-coumarate--CoA ligase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=4CL3 PE=1 ...	[more]
Q9S725	1.1e-36	34.08	4-coumarate--CoA ligase 2 OS=Arabidopsis thaliana OX=3702 GN=4CL2 PE=1 SV=2	[more]

BLAST of Carg24032 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G8F9	5.2e-71	56.51	4-coumarate--CoA ligase 3 OS=Cucurbita moschata OX=3662 GN=LOC111451685 PE=4 SV=...	[more]
A0A5A7THZ8	1.2e-51	43.81	4-coumarate--CoA ligase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3CJB3	1.2e-51	43.81	4-coumarate--CoA ligase 2 OS=Cucumis melo OX=3656 GN=LOC103501067 PE=4 SV=1	[more]
A0A1U8B782	8.9e-47	47.72	4-coumarate--CoA ligase 2-like OS=Nelumbo nucifera OX=4432 GN=LOC104611494 PE=4 ...	[more]
A0A6J1DCS3	1.5e-46	42.76	4-coumarate--CoA ligase 2-like OS=Momordica charantia OX=3673 GN=LOC111018921 PE...	[more]

BLAST of Carg24032 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G65060.1	1.3e-45	34.87	4-coumarate:CoA ligase 3	[more]
AT1G65060.2	2.0e-38	49.70	4-coumarate:CoA ligase 3	[more]
AT3G21240.1	7.5e-38	34.08	4-coumarate:CoA ligase 2	[more]
AT1G51680.1	7.0e-36	32.62	4-coumarate:CoA ligase 1	[more]
AT1G51680.3	1.6e-35	33.55	4-coumarate:CoA ligase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.980		coord: 17..158 e-value: 1.7E-41 score: 143.5
None	No IPR available	GENE3D	3.30.300.30		coord: 159..229 e-value: 3.3E-13 score: 52.1
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 28..158 coord: 158..216
None	No IPR available	PANTHER	PTHR24096:SF169	4-COUMARATE--COA LIGASE 3	coord: 28..158 coord: 158..216
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 50..215
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 49..155 e-value: 2.3E-19 score: 69.3
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 163..215 e-value: 1.2E-11 score: 45.4

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24032-RA	Carg24032-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016405	CoA-ligase activity