Carg23971 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg23971 vs. NCBI nr
Match: KAG7020643.1 (Endoglucanase 16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 800.8 bits (2067), Expect = 5.2e-228
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 0

Query: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60
           MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSALDDGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTL 120
           PAWRGDSALDDGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTL
Sbjct: 61  PAWRGDSALDDGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTL 120

Query: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180
           SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN
Sbjct: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180

Query: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240
           MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK
Sbjct: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240

Query: 241 GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300
           GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG
Sbjct: 241 GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300

Query: 301 AQVLLSKADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAY 360
           AQVLLSKADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAY
Sbjct: 301 AQVLLSKADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAY 360

Query: 361 KQNVKCSDQQFDPAHLMTFAKKQAERNRTNPS 393
           KQNVKCSDQQFDPAHLMTFAKKQAERNRTNPS
Sbjct: 361 KQNVKCSDQQFDPAHLMTFAKKQAERNRTNPS 392

BLAST of Carg23971 vs. NCBI nr
Match: XP_022951947.1 (endoglucanase 16-like [Cucurbita moschata])

HSP 1 Score: 724.9 bits (1870), Expect = 3.6e-205
Identity = 365/400 (91.25%), Postives = 366/400 (91.50%), Query Frame = 0

Query: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60
           MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSALDDGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTL 120
           PAWRGDSALDDGKLAN                 VDLVGGYYDAGDNVKYGLPMAFTVTTL
Sbjct: 61  PAWRGDSALDDGKLAN-----------------VDLVGGYYDAGDNVKYGLPMAFTVTTL 120

Query: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180
           SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN
Sbjct: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180

Query: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240
           MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK
Sbjct: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240

Query: 241 GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300
            TYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG
Sbjct: 241 ATYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300

Query: 301 AQVLLSK---------------ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360
           AQVLLSK               ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG
Sbjct: 301 AQVLLSKLYFEGEKGLETFKNQADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360

Query: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQ +
Sbjct: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQMD 383

BLAST of Carg23971 vs. NCBI nr
Match: XP_023537900.1 (endoglucanase 16-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 714.1 bits (1842), Expect = 6.4e-202
Identity = 359/400 (89.75%), Postives = 364/400 (91.00%), Query Frame = 0

Query: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60
           MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAA+FNYKDALTKSLIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAAEFNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSALDDGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTL 120
           PAWRGDSALDDGKLAN                 VDLVGGYYDAGDNVKYGLPMAFTVTTL
Sbjct: 61  PAWRGDSALDDGKLAN-----------------VDLVGGYYDAGDNVKYGLPMAFTVTTL 120

Query: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180
           SWGALTYP ELEAAGEMENLKAAI+WGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN
Sbjct: 121 SWGALTYPTELEAAGEMENLKAAIRWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180

Query: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240
           MKTPRTVLQI+SETPGTEIAAETSAAMASSS+VFR SNQTYARLLLNKAKTLYKFAKAHK
Sbjct: 181 MKTPRTVLQINSETPGTEIAAETSAAMASSSMVFRSSNQTYARLLLNKAKTLYKFAKAHK 240

Query: 241 GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300
           GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYV EESISASVAEFSWDLKYVG
Sbjct: 241 GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVQEESISASVAEFSWDLKYVG 300

Query: 301 AQVLLSK---------------ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360
           AQVLLSK               ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG
Sbjct: 301 AQVLLSKLYFEGEKGLETFKNQADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360

Query: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQ +
Sbjct: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQMD 383

BLAST of Carg23971 vs. NCBI nr
Match: XP_023002656.1 (endoglucanase 16-like [Cucurbita maxima])

HSP 1 Score: 709.9 bits (1831), Expect = 1.2e-200
Identity = 356/400 (89.00%), Postives = 362/400 (90.50%), Query Frame = 0

Query: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60
           MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAA+ NYKDALTKSLIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAAELNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSALDDGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTL 120
           PAWRGDSALDDGKLAN                 VDLVGGYYDAGDNVKYGLPMAFTVTTL
Sbjct: 61  PAWRGDSALDDGKLAN-----------------VDLVGGYYDAGDNVKYGLPMAFTVTTL 120

Query: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180
           SWGALTYP ELEAAGEMEN+KAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN
Sbjct: 121 SWGALTYPTELEAAGEMENVKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180

Query: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240
           MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFR SNQTYARLLLNKAKTLYKFAK+HK
Sbjct: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRSSNQTYARLLLNKAKTLYKFAKSHK 240

Query: 241 GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300
           GTYDGECPFYCSYSGYNDELLWAATWLY+ATRKSVYLKYVLEESISASVAEFSWDLKYVG
Sbjct: 241 GTYDGECPFYCSYSGYNDELLWAATWLYIATRKSVYLKYVLEESISASVAEFSWDLKYVG 300

Query: 301 AQVLLSK---------------ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360
           AQVLLSK               AD+YICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG
Sbjct: 301 AQVLLSKLYFEGEKGLETFKNQADNYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360

Query: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           TAFVFSAYSDILAAYKQNVKCSDQQFD  HLMTFAKKQ +
Sbjct: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDSTHLMTFAKKQMD 383

BLAST of Carg23971 vs. NCBI nr
Match: KAG6585735.1 (Endoglucanase 16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 704.9 bits (1818), Expect = 3.9e-199
Identity = 359/400 (89.75%), Postives = 360/400 (90.00%), Query Frame = 0

Query: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60
           MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSALDDGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTL 120
           PAWRGDSALDDGKLAN                 VDLVGGYYDAGDNVKYGLPMAFTVTTL
Sbjct: 61  PAWRGDSALDDGKLAN-----------------VDLVGGYYDAGDNVKYGLPMAFTVTTL 120

Query: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180
           SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVE      DHECWVRPEN
Sbjct: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVE------DHECWVRPEN 180

Query: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240
           MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK
Sbjct: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240

Query: 241 GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300
           GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG
Sbjct: 241 GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300

Query: 301 AQVLLSK---------------ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360
           AQVLLSK               ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG
Sbjct: 301 AQVLLSKLYFEGEKGLETFKNQADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360

Query: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           TAFVFSAYSDILAAYKQNVKCSDQQFD AHLMTFAKKQ +
Sbjct: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDSAHLMTFAKKQMD 377

BLAST of Carg23971 vs. ExPASy Swiss-Prot
Match: Q8VYG3 (Endoglucanase 16 OS=Arabidopsis thaliana OX=3702 GN=At3g43860 PE=2 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 1.4e-143
Identity = 245/372 (65.86%), Postives = 285/372 (76.61%), Query Frame = 0

Query: 29  VHAADFNYKDALTKSLIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANRKHTDKDLHGAE 88
           V+    NYKDALTKSLIFLEAQRSGKLPPN+R  WRGDSALDDGKL N            
Sbjct: 28  VNGTFINYKDALTKSLIFLEAQRSGKLPPNNRVPWRGDSALDDGKLVN------------ 87

Query: 89  RRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELEAAGEMENLKAAIKWGT 148
                VDL GGYYDAGDNVKYGLPMAFT+TTL+W  +TY  EL A GE+EN +AAI+WGT
Sbjct: 88  -----VDLSGGYYDAGDNVKYGLPMAFTITTLAWSTITYEKELRATGELENARAAIRWGT 147

Query: 149 DYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGTEIAAETSAAMA 208
           DYFLK +S ++RLYV+VGDP  DH+CW RPENMKTPRTVL+I  + PGTEIAAE +AA A
Sbjct: 148 DYFLKCASRKNRLYVQVGDPNADHQCWARPENMKTPRTVLEISDKVPGTEIAAEAAAAFA 207

Query: 209 SSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKGTYDGECPFYCSYSGYNDELLWAATWLY 268
           +SSIVFRH +  YAR LLNKAK L+K AK+HKGTYDGECPFYCS SGYNDEL+WAATWLY
Sbjct: 208 ASSIVFRHVDHKYARRLLNKAKLLFKLAKSHKGTYDGECPFYCSNSGYNDELIWAATWLY 267

Query: 269 VATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLSK---------------ADSYIC 328
            ATR  +YL Y+  E+ISA VAEFSWDLKY GAQ+L++K               ADS++C
Sbjct: 268 KATRNHLYLSYLKFEAISAYVAEFSWDLKYAGAQILITKLIFEGHKGLDLYKQQADSFVC 327

Query: 329 SNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAYKQNVKCSDQQFDP 386
           SNLP SPYHQ++ +PGGM+H+RDGAN+QYVT TAF+FSAY+DIL  + Q + C   QFD 
Sbjct: 328 SNLPGSPYHQVFTTPGGMIHLRDGANSQYVTATAFLFSAYADILQKHNQKISCGSHQFDS 382

BLAST of Carg23971 vs. ExPASy Swiss-Prot
Match: Q6K7G9 (Endoglucanase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0778600 PE=2 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 5.9e-134
Identity = 237/398 (59.55%), Postives = 299/398 (75.13%), Query Frame = 0

Query: 6   HN-LSAMAILVGSLLFFQPFFPGAV--HAADFNYKDALTKSLIFLEAQRSGKLPPNHRPA 65
           HN  +A A+L+ +L+      P  V   A  FNYKDALTKS++FLEAQRSGKLPP +R  
Sbjct: 10  HNAAAAAALLLAALVLSGDVLPAVVAGGAPSFNYKDALTKSIMFLEAQRSGKLPPTNRIK 69

Query: 66  WRGDSALDDGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTLSW 125
           WRGDS ++DGKLAN                 VDL GGYYDAGDNVKYGLP+AFTVTTL+W
Sbjct: 70  WRGDSGMEDGKLAN-----------------VDLTGGYYDAGDNVKYGLPLAFTVTTLAW 129

Query: 126 GALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMK 185
            A+ +  EL+AA E+EN+ AAI+WGTDYFLKA++ +D L+V+VGDP  DH+CWVRPENM 
Sbjct: 130 TAMAFEKELKAARELENVHAAIRWGTDYFLKAATKKDHLWVQVGDPNADHQCWVRPENMP 189

Query: 186 TPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKGT 245
           TPRT+ QI+ +TPG+EIAAET+AAM +SS+VFR  ++ Y+R LLNKAK L++FAK H+GT
Sbjct: 190 TPRTLYQINDKTPGSEIAAETAAAMTASSMVFR-KDKPYSRRLLNKAKLLFQFAKTHQGT 249

Query: 246 YDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVGAQ 305
           YDGECPFYCSYSGYNDELLWAATWLY+AT++ VY  ++  E+IS+SVAEFSWDLK+ GAQ
Sbjct: 250 YDGECPFYCSYSGYNDELLWAATWLYLATKRQVYADFIGHEAISSSVAEFSWDLKFPGAQ 309

Query: 306 VLL---------------SKADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTA 365
           VLL               S+AD+++C+ LP +P+HQ+ ++PGGM+H+RDGAN+QYVT TA
Sbjct: 310 VLLAELNMTSSGGLQSFKSQADNFVCAVLPDTPFHQVSITPGGMIHLRDGANSQYVTSTA 369

Query: 366 FVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           F+F AYSDIL    Q V C  Q   PA L+ FAK+Q +
Sbjct: 370 FLFVAYSDILRRINQPVMCGAQAVQPARLLQFAKQQID 389

BLAST of Carg23971 vs. ExPASy Swiss-Prot
Match: P22503 (Endoglucanase OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 337.0 bits (863), Expect = 2.8e-91
Identity = 169/370 (45.68%), Postives = 237/370 (64.05%), Query Frame = 0

Query: 33  DFNYKDALTKSLIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANRKHTDKDLHGAERRNS 92
           +++Y DAL K+++F E QRSGKLP + R  WR DSAL DGKL N                
Sbjct: 38  NYDYADALAKAILFFEGQRSGKLPSSQRVKWREDSALSDGKLQN---------------- 97

Query: 93  PVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELEAAGEMENLKAAIKWGTDYFL 152
            V+L+GGYYDAGDNVK+G PMAF+ + LSW A+ Y +E+ +  ++  L++AI+WG D+ L
Sbjct: 98  -VNLMGGYYDAGDNVKFGWPMAFSTSLLSWAAVEYESEISSVNQLGYLQSAIRWGADFML 157

Query: 153 KASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGTEIAAETSAAMASSSI 212
           +A +    LY +VGD   DH CW RPE+M TPRTV +ID+ +PGTE+AAE +AA++++SI
Sbjct: 158 RAHTSPTTLYTQVGDGNADHNCWERPEDMDTPRTVYKIDANSPGTEVAAEYAAALSAASI 217

Query: 213 VFRHSNQTYARLLLNKAKTLYKFAKAHKGTYDGECPFYCSYSGYNDELLWAATWLYVATR 272
           VF+  +  Y+  LL+ +K+L+ FA  ++G+Y G CPFYCSYSGY DELLWAA WLY A+ 
Sbjct: 218 VFKKIDAKYSSTLLSHSKSLFDFADKNRGSYSGSCPFYCSYSGYQDELLWAAAWLYKASG 277

Query: 273 KSVYLKYVL-EESISASVAEFSWDLKYVGAQVLLSK---------------ADSYICSNL 332
           +S YL Y++  +  S +V+EFSWD K+VGAQ LL++               A+S+IC+ +
Sbjct: 278 ESKYLSYIISNQGWSQTVSEFSWDNKFVGAQTLLTEEFYGGKKDLAKIKTDAESFICAVM 337

Query: 333 PTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAYKQN-VKCSDQQFDPAH 386
           P S   QI  +PGG++  RD +N QY T +  V   +S IL     N + C    F  + 
Sbjct: 338 PGSNSRQIKTTPGGLLFTRDSSNLQYTTSSTMVLFIFSRILNRNHINGINCGSSHFTASQ 390

BLAST of Carg23971 vs. ExPASy Swiss-Prot
Match: O81416 (Endoglucanase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g02290 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 8.1e-91
Identity = 172/381 (45.14%), Postives = 233/381 (61.15%), Query Frame = 0

Query: 30  HAADFNYKDALTKSLIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANRKHTDKDLHGAER 89
           H A  NYKDALTKS++F E QRSGKLP N R +WR DS L DG           LH    
Sbjct: 47  HLAKHNYKDALTKSILFFEGQRSGKLPSNQRMSWRRDSGLSDGSA---------LH---- 106

Query: 90  RNSPVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELEAAGEMENLKAAIKWGTD 149
               VDLVGGYYDAGDN+K+G PMAFT T LSW  + +   +++  E++N K AI+W TD
Sbjct: 107 ----VDLVGGYYDAGDNIKFGFPMAFTTTMLSWSVIEFGGLMKS--ELQNAKIAIRWATD 166

Query: 150 YFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGTEIAAETSAAMAS 209
           Y LKA+S  D +YV+VGD  KDH CW RPE+M T R+V ++D   PG+++AAET+AA+A+
Sbjct: 167 YLLKATSQPDTIYVQVGDANKDHSCWERPEDMDTVRSVFKVDKNIPGSDVAAETAAALAA 226

Query: 210 SSIVFRHSNQTYARLLLNKAKTLYKFAKAHKGTYDG-----ECPFYCSYSGYNDELLWAA 269
           ++IVFR S+ +Y+++LL +A +++ FA  ++GTY        CPFYCSYSGY DELLW A
Sbjct: 227 AAIVFRKSDPSYSKVLLKRAISVFAFADKYRGTYSAGLKPDVCPFYCSYSGYQDELLWGA 286

Query: 270 TWLYVATRKSVYLKYVLEESISASVAE----FSWDLKYVGAQVLLSK------------- 329
            WL  AT+   YL Y+         AE    F WD K+ GA++LL+K             
Sbjct: 287 AWLQKATKNIKYLNYIKINGQILGAAEYDNTFGWDNKHAGARILLTKAFLVQNVKTLHEY 346

Query: 330 ---ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAYKQNV 386
              AD++ICS +P +P+     +PGG++     AN QYVT T+F+   Y+  L + K  V
Sbjct: 347 KGHADNFICSVIPGAPFSSTQYTPGGLLFKMADANMQYVTSTSFLLLTYAKYLTSAKTVV 406

BLAST of Carg23971 vs. ExPASy Swiss-Prot
Match: Q9SUS0 (Endoglucanase 20 OS=Arabidopsis thaliana OX=3702 GN=At4g23560 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.1e-90
Identity = 175/390 (44.87%), Postives = 235/390 (60.26%), Query Frame = 0

Query: 13  ILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHRPAWRGDSALDDG 72
           +LVG  L F+             Y DAL KS++F E QRSGKLP N R  WR DSAL DG
Sbjct: 8   MLVGMFLAFESL-------EALEYGDALNKSILFFEGQRSGKLPTNQRVKWRADSALSDG 67

Query: 73  KLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELE 132
            LAN                 V+L+GGYYDAGDNVK+  PM+FT T LSW A+ Y  E+ 
Sbjct: 68  SLAN-----------------VNLIGGYYDAGDNVKFVWPMSFTTTLLSWAAIEYQNEIS 127

Query: 133 AAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDS 192
           +  ++  L++ IKWGTD+ L+A +  + LY +VGD   DH CW RPE+M T RT+  I S
Sbjct: 128 SVNQLGYLRSTIKWGTDFILRAHTSPNMLYTQVGDGNSDHSCWERPEDMDTSRTLYSISS 187

Query: 193 ETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKGTYDGECPFYCS 252
            +PG+E A E +AA+A++S+VF+  + TY+  LLN AKTL++FA  ++G+Y   CPFYCS
Sbjct: 188 SSPGSEAAGEAAAALAAASLVFKSVDSTYSSTLLNHAKTLFEFADKYRGSYQASCPFYCS 247

Query: 253 YSGYNDELLWAATWLYVATRKSVYLKYVL-EESISASVAEFSWDLKYVGAQVLL------ 312
           YSGY DELLWAA WLY AT   +Y+ YV+  +  S +V EFSWD K+VGAQ LL      
Sbjct: 248 YSGYQDELLWAAAWLYKATGDKIYINYVISNKDWSQAVNEFSWDNKFVGAQALLVSEFYN 307

Query: 313 ---------SKADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDI 372
                    S  +S++C+ +P S   QI  +PGG++ +RD +N QYVT    V   YS  
Sbjct: 308 GANDLAKFKSDVESFVCAMMPGSSSQQIKPTPGGLLFIRDSSNLQYVTTATTVLFHYSKT 367

Query: 373 LA-AYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           L  A   +++C   +F  + +  FAK Q +
Sbjct: 368 LTKAGVGSIQCGSTKFTVSQIRNFAKSQVD 373

BLAST of Carg23971 vs. ExPASy TrEMBL
Match: A0A6J1GJ20 (Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111454680 PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 1.8e-205
Identity = 365/400 (91.25%), Postives = 366/400 (91.50%), Query Frame = 0

Query: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60
           MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSALDDGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTL 120
           PAWRGDSALDDGKLAN                 VDLVGGYYDAGDNVKYGLPMAFTVTTL
Sbjct: 61  PAWRGDSALDDGKLAN-----------------VDLVGGYYDAGDNVKYGLPMAFTVTTL 120

Query: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180
           SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN
Sbjct: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180

Query: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240
           MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK
Sbjct: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240

Query: 241 GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300
            TYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG
Sbjct: 241 ATYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300

Query: 301 AQVLLSK---------------ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360
           AQVLLSK               ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG
Sbjct: 301 AQVLLSKLYFEGEKGLETFKNQADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360

Query: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQ +
Sbjct: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQMD 383

BLAST of Carg23971 vs. ExPASy TrEMBL
Match: A0A6J1KLX1 (Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111496447 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 5.8e-201
Identity = 356/400 (89.00%), Postives = 362/400 (90.50%), Query Frame = 0

Query: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60
           MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAA+ NYKDALTKSLIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAAELNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSALDDGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTL 120
           PAWRGDSALDDGKLAN                 VDLVGGYYDAGDNVKYGLPMAFTVTTL
Sbjct: 61  PAWRGDSALDDGKLAN-----------------VDLVGGYYDAGDNVKYGLPMAFTVTTL 120

Query: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180
           SWGALTYP ELEAAGEMEN+KAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN
Sbjct: 121 SWGALTYPTELEAAGEMENVKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180

Query: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240
           MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFR SNQTYARLLLNKAKTLYKFAK+HK
Sbjct: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRSSNQTYARLLLNKAKTLYKFAKSHK 240

Query: 241 GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300
           GTYDGECPFYCSYSGYNDELLWAATWLY+ATRKSVYLKYVLEESISASVAEFSWDLKYVG
Sbjct: 241 GTYDGECPFYCSYSGYNDELLWAATWLYIATRKSVYLKYVLEESISASVAEFSWDLKYVG 300

Query: 301 AQVLLSK---------------ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360
           AQVLLSK               AD+YICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG
Sbjct: 301 AQVLLSKLYFEGEKGLETFKNQADNYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360

Query: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           TAFVFSAYSDILAAYKQNVKCSDQQFD  HLMTFAKKQ +
Sbjct: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDSTHLMTFAKKQMD 383

BLAST of Carg23971 vs. ExPASy TrEMBL
Match: A0A6J1BR89 (Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111004858 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.2e-168
Identity = 301/400 (75.25%), Postives = 329/400 (82.25%), Query Frame = 0

Query: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60
           MG +   LS  AI VG L  FQ F    V A + NYKDAL+K+LIFLEAQRSGKLPPNHR
Sbjct: 1   MGALKMKLSTTAIAVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHR 60

Query: 61  PAWRGDSALDDGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTL 120
           PAWRGDSA+DDGKLAN                 VDL GGYYDAGDNVKYGLPMAFTVTTL
Sbjct: 61  PAWRGDSAVDDGKLAN-----------------VDLEGGYYDAGDNVKYGLPMAFTVTTL 120

Query: 121 SWGALTYPAELEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPEN 180
           SW A+TY AE+EAAGEMEN++AAIKWGTDYFLKA S R+RLYV+VGDPVKDHECWVRPEN
Sbjct: 121 SWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPEN 180

Query: 181 MKTPRTVLQIDSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHK 240
           MKTPRTVLQID+ TPGTEIAAETSAAMASSSIVFR  N+TYAR LLNKAK L++FAKAHK
Sbjct: 181 MKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHK 240

Query: 241 GTYDGECPFYCSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVG 300
           GTYDGECPFYCS+SGYNDELLWAATWLY+AT++ VYLKYV EE+I+ SVAEFSWDLKY G
Sbjct: 241 GTYDGECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAG 300

Query: 301 AQVLLSK---------------ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTG 360
           AQ+LLSK               ADSYICSNLP SPYHQIYVSPGGM+HMRDGANTQYVTG
Sbjct: 301 AQILLSKFYFEGEKGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTG 360

Query: 361 TAFVFSAYSDILAAYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           TAF+FSAYSDILA +KQ VKC+DQQFD   LM+FAKKQ +
Sbjct: 361 TAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQMD 380

BLAST of Carg23971 vs. ExPASy TrEMBL
Match: A0A6J1EYP8 (Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111439768 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 7.2e-167
Identity = 295/390 (75.64%), Postives = 321/390 (82.31%), Query Frame = 0

Query: 11  MAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHRPAWRGDSALD 70
           M IL+ SLL FQ  F   +HAA FNYKDALTK LIFLEAQRSGKLPPNHRP WRGDSALD
Sbjct: 1   MPILLPSLLLFQALFLAPLHAAQFNYKDALTKCLIFLEAQRSGKLPPNHRPPWRGDSALD 60

Query: 71  DGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAE 130
           DGKLAN                 VDL GGYYDAGDNVKYGLPMAFTVTTL+W AL YPAE
Sbjct: 61  DGKLAN-----------------VDLEGGYYDAGDNVKYGLPMAFTVTTLAWAALAYPAE 120

Query: 131 LEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQI 190
            +AAGEMENLKAAI+WGTDY LKASS R+R YVEVGDPVKDHECW+RPENMKTPRTVLQI
Sbjct: 121 TQAAGEMENLKAAIQWGTDYLLKASSRRNRFYVEVGDPVKDHECWIRPENMKTPRTVLQI 180

Query: 191 DSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKGTYDGECPFY 250
           D  TPGTEIAAETSAAMASSSIVFR+SN+TY+R LLNKAKTL++FAK ++GTYDGECPFY
Sbjct: 181 DRTTPGTEIAAETSAAMASSSIVFRNSNRTYSRRLLNKAKTLFQFAKMNQGTYDGECPFY 240

Query: 251 CSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLS---- 310
           CSYSGYNDELLWAATWLY+ATR+  Y KY+ EES+S SVAEFSWDLKY GAQ+LLS    
Sbjct: 241 CSYSGYNDELLWAATWLYIATRRPAYFKYITEESVSGSVAEFSWDLKYAGAQILLSEIYF 300

Query: 311 -----------KADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSD 370
                      +ADSYICSNLP SPYHQIY+SPGGMVHMRDGANTQYVTGTAF+FSAYSD
Sbjct: 301 QGQKGLQMYKNQADSYICSNLPNSPYHQIYLSPGGMVHMRDGANTQYVTGTAFLFSAYSD 360

Query: 371 ILAAYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           ILA ++Q V+C  QQFD   LMTFAKKQ +
Sbjct: 361 ILATHQQTVQCGGQQFDSTQLMTFAKKQMD 373

BLAST of Carg23971 vs. ExPASy TrEMBL
Match: A0A6J1KB06 (Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111492677 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 9.4e-167
Identity = 295/390 (75.64%), Postives = 321/390 (82.31%), Query Frame = 0

Query: 11  MAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHRPAWRGDSALD 70
           M IL+ SLL FQ  F   +HAA FNYKDALTK LIFLEAQRSGKLPPNHRP WRGDSALD
Sbjct: 1   MPILLPSLLLFQALFLAPLHAAQFNYKDALTKCLIFLEAQRSGKLPPNHRPPWRGDSALD 60

Query: 71  DGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAE 130
           DGKLAN                 VDL GGYYDAGDNVKYGLPMAFTVTTL+W AL YPAE
Sbjct: 61  DGKLAN-----------------VDLEGGYYDAGDNVKYGLPMAFTVTTLAWAALAYPAE 120

Query: 131 LEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQI 190
            +AAGEMENLKAAI+WGTDY LKASS R+R YVEVGDPVKDHECW+RPENMKTPRTVLQI
Sbjct: 121 TQAAGEMENLKAAIQWGTDYLLKASSRRNRFYVEVGDPVKDHECWIRPENMKTPRTVLQI 180

Query: 191 DSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKGTYDGECPFY 250
           D  TPGTEIAAETSAAMASSSIVFR+SN+TY+R LLNKAK L++FAK ++GTYDGECPFY
Sbjct: 181 DRTTPGTEIAAETSAAMASSSIVFRNSNRTYSRRLLNKAKLLFQFAKMNRGTYDGECPFY 240

Query: 251 CSYSGYNDELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLS---- 310
           CSYSGYNDELLWAATWLY+ATR+  YLKY+ EES+S SVAEFSWDLKY GAQ+LLS    
Sbjct: 241 CSYSGYNDELLWAATWLYIATRRPAYLKYITEESVSGSVAEFSWDLKYAGAQILLSEIYF 300

Query: 311 -----------KADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSD 370
                      +ADSYICSNLP SPYHQIY+SPGGMVHMRDGANTQYVTGTAF+FSAYSD
Sbjct: 301 QGEKGLQMYKNQADSYICSNLPNSPYHQIYLSPGGMVHMRDGANTQYVTGTAFLFSAYSD 360

Query: 371 ILAAYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           ILA ++Q V+C  QQFD   LMTFAKKQ +
Sbjct: 361 ILATHQQTVQCGGQQFDSTQLMTFAKKQMD 373

BLAST of Carg23971 vs. TAIR 10
Match: AT3G43860.1 (glycosyl hydrolase 9A4 )

HSP 1 Score: 510.8 bits (1314), Expect = 1.0e-144
Identity = 245/372 (65.86%), Postives = 285/372 (76.61%), Query Frame = 0

Query: 29  VHAADFNYKDALTKSLIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANRKHTDKDLHGAE 88
           V+    NYKDALTKSLIFLEAQRSGKLPPN+R  WRGDSALDDGKL N            
Sbjct: 28  VNGTFINYKDALTKSLIFLEAQRSGKLPPNNRVPWRGDSALDDGKLVN------------ 87

Query: 89  RRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELEAAGEMENLKAAIKWGT 148
                VDL GGYYDAGDNVKYGLPMAFT+TTL+W  +TY  EL A GE+EN +AAI+WGT
Sbjct: 88  -----VDLSGGYYDAGDNVKYGLPMAFTITTLAWSTITYEKELRATGELENARAAIRWGT 147

Query: 149 DYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGTEIAAETSAAMA 208
           DYFLK +S ++RLYV+VGDP  DH+CW RPENMKTPRTVL+I  + PGTEIAAE +AA A
Sbjct: 148 DYFLKCASRKNRLYVQVGDPNADHQCWARPENMKTPRTVLEISDKVPGTEIAAEAAAAFA 207

Query: 209 SSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKGTYDGECPFYCSYSGYNDELLWAATWLY 268
           +SSIVFRH +  YAR LLNKAK L+K AK+HKGTYDGECPFYCS SGYNDEL+WAATWLY
Sbjct: 208 ASSIVFRHVDHKYARRLLNKAKLLFKLAKSHKGTYDGECPFYCSNSGYNDELIWAATWLY 267

Query: 269 VATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLSK---------------ADSYIC 328
            ATR  +YL Y+  E+ISA VAEFSWDLKY GAQ+L++K               ADS++C
Sbjct: 268 KATRNHLYLSYLKFEAISAYVAEFSWDLKYAGAQILITKLIFEGHKGLDLYKQQADSFVC 327

Query: 329 SNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAYKQNVKCSDQQFDP 386
           SNLP SPYHQ++ +PGGM+H+RDGAN+QYVT TAF+FSAY+DIL  + Q + C   QFD 
Sbjct: 328 SNLPGSPYHQVFTTPGGMIHLRDGANSQYVTATAFLFSAYADILQKHNQKISCGSHQFDS 382

BLAST of Carg23971 vs. TAIR 10
Match: AT4G02290.1 (glycosyl hydrolase 9B13 )

HSP 1 Score: 335.5 bits (859), Expect = 5.8e-92
Identity = 172/381 (45.14%), Postives = 233/381 (61.15%), Query Frame = 0

Query: 30  HAADFNYKDALTKSLIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANRKHTDKDLHGAER 89
           H A  NYKDALTKS++F E QRSGKLP N R +WR DS L DG           LH    
Sbjct: 47  HLAKHNYKDALTKSILFFEGQRSGKLPSNQRMSWRRDSGLSDGSA---------LH---- 106

Query: 90  RNSPVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELEAAGEMENLKAAIKWGTD 149
               VDLVGGYYDAGDN+K+G PMAFT T LSW  + +   +++  E++N K AI+W TD
Sbjct: 107 ----VDLVGGYYDAGDNIKFGFPMAFTTTMLSWSVIEFGGLMKS--ELQNAKIAIRWATD 166

Query: 150 YFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGTEIAAETSAAMAS 209
           Y LKA+S  D +YV+VGD  KDH CW RPE+M T R+V ++D   PG+++AAET+AA+A+
Sbjct: 167 YLLKATSQPDTIYVQVGDANKDHSCWERPEDMDTVRSVFKVDKNIPGSDVAAETAAALAA 226

Query: 210 SSIVFRHSNQTYARLLLNKAKTLYKFAKAHKGTYDG-----ECPFYCSYSGYNDELLWAA 269
           ++IVFR S+ +Y+++LL +A +++ FA  ++GTY        CPFYCSYSGY DELLW A
Sbjct: 227 AAIVFRKSDPSYSKVLLKRAISVFAFADKYRGTYSAGLKPDVCPFYCSYSGYQDELLWGA 286

Query: 270 TWLYVATRKSVYLKYVLEESISASVAE----FSWDLKYVGAQVLLSK------------- 329
            WL  AT+   YL Y+         AE    F WD K+ GA++LL+K             
Sbjct: 287 AWLQKATKNIKYLNYIKINGQILGAAEYDNTFGWDNKHAGARILLTKAFLVQNVKTLHEY 346

Query: 330 ---ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAYKQNV 386
              AD++ICS +P +P+     +PGG++     AN QYVT T+F+   Y+  L + K  V
Sbjct: 347 KGHADNFICSVIPGAPFSSTQYTPGGLLFKMADANMQYVTSTSFLLLTYAKYLTSAKTVV 406

BLAST of Carg23971 vs. TAIR 10
Match: AT4G23560.1 (glycosyl hydrolase 9B15 )

HSP 1 Score: 335.1 bits (858), Expect = 7.5e-92
Identity = 175/390 (44.87%), Postives = 235/390 (60.26%), Query Frame = 0

Query: 13  ILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHRPAWRGDSALDDG 72
           +LVG  L F+             Y DAL KS++F E QRSGKLP N R  WR DSAL DG
Sbjct: 8   MLVGMFLAFESL-------EALEYGDALNKSILFFEGQRSGKLPTNQRVKWRADSALSDG 67

Query: 73  KLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELE 132
            LAN                 V+L+GGYYDAGDNVK+  PM+FT T LSW A+ Y  E+ 
Sbjct: 68  SLAN-----------------VNLIGGYYDAGDNVKFVWPMSFTTTLLSWAAIEYQNEIS 127

Query: 133 AAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDS 192
           +  ++  L++ IKWGTD+ L+A +  + LY +VGD   DH CW RPE+M T RT+  I S
Sbjct: 128 SVNQLGYLRSTIKWGTDFILRAHTSPNMLYTQVGDGNSDHSCWERPEDMDTSRTLYSISS 187

Query: 193 ETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKGTYDGECPFYCS 252
            +PG+E A E +AA+A++S+VF+  + TY+  LLN AKTL++FA  ++G+Y   CPFYCS
Sbjct: 188 SSPGSEAAGEAAAALAAASLVFKSVDSTYSSTLLNHAKTLFEFADKYRGSYQASCPFYCS 247

Query: 253 YSGYNDELLWAATWLYVATRKSVYLKYVL-EESISASVAEFSWDLKYVGAQVLL------ 312
           YSGY DELLWAA WLY AT   +Y+ YV+  +  S +V EFSWD K+VGAQ LL      
Sbjct: 248 YSGYQDELLWAAAWLYKATGDKIYINYVISNKDWSQAVNEFSWDNKFVGAQALLVSEFYN 307

Query: 313 ---------SKADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDI 372
                    S  +S++C+ +P S   QI  +PGG++ +RD +N QYVT    V   YS  
Sbjct: 308 GANDLAKFKSDVESFVCAMMPGSSSQQIKPTPGGLLFIRDSSNLQYVTTATTVLFHYSKT 367

Query: 373 LA-AYKQNVKCSDQQFDPAHLMTFAKKQAE 386
           L  A   +++C   +F  + +  FAK Q +
Sbjct: 368 LTKAGVGSIQCGSTKFTVSQIRNFAKSQVD 373

BLAST of Carg23971 vs. TAIR 10
Match: AT1G02800.1 (cellulase 2 )

HSP 1 Score: 325.9 bits (834), Expect = 4.6e-89
Identity = 172/381 (45.14%), Postives = 225/381 (59.06%), Query Frame = 0

Query: 30  HAADFNYKDALTKSLIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANRKHTDKDLHGAER 89
           H  + NYKDAL+KS++F E QRSGKLPPN R  WR +S L DG   N             
Sbjct: 38  HLDNHNYKDALSKSILFFEGQRSGKLPPNQRMTWRSNSGLSDGSALN------------- 97

Query: 90  RNSPVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELEAAGEMENLKAAIKWGTD 149
               VDLVGGYYDAGDN+K+G PMAFT T LSW  + +   +++  E+ N K AI+W TD
Sbjct: 98  ----VDLVGGYYDAGDNMKFGFPMAFTTTMLSWSLIEFGGLMKS--ELPNAKDAIRWATD 157

Query: 150 YFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGTEIAAETSAAMAS 209
           + LKA+SH D +YV+VGDP  DH CW RPE+M TPR+V ++D   PG++IA E +AA+A+
Sbjct: 158 FLLKATSHPDTIYVQVGDPNMDHACWERPEDMDTPRSVFKVDKNNPGSDIAGEIAAALAA 217

Query: 210 SSIVFRHSNQTYARLLLNKAKTLYKFAKAHKGTYDG-----ECPFYCSYSGYNDELLWAA 269
           +SIVFR  + +Y+  LL +A T++ FA  ++G Y        CPFYCSYSGY DELLW A
Sbjct: 218 ASIVFRKCDPSYSNHLLQRAITVFTFADKYRGPYSAGLAPEVCPFYCSYSGYQDELLWGA 277

Query: 270 TWLYVATRKSVYLKYVLEESISASVAE----FSWDLKYVGAQVLLSK------------- 329
            WL  AT    YL Y+          E    FSWD K+VGA++LLSK             
Sbjct: 278 AWLQKATNNPTYLNYIKANGQILGADEFDNMFSWDNKHVGARILLSKEFLIQKVKSLEEY 337

Query: 330 ---ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILAAYKQNV 386
              ADS+ICS LP +   Q   +PGG++     +N QYVT T+F+   Y+  L + +   
Sbjct: 338 KEHADSFICSVLPGASSSQ--YTPGGLLFKMGESNMQYVTSTSFLLLTYAKYLTSARTVA 397

BLAST of Carg23971 vs. TAIR 10
Match: AT4G09740.1 (glycosyl hydrolase 9B14 )

HSP 1 Score: 324.3 bits (830), Expect = 1.3e-88
Identity = 170/392 (43.37%), Postives = 236/392 (60.20%), Query Frame = 0

Query: 11  MAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHRPAWRGDSALD 70
           + +L+G  L F+     ++ A D  Y DAL KS++F E QRSGKLP N R  WR DS L 
Sbjct: 6   VVMLIGMFLAFE-----SLEALD--YGDALNKSILFFEGQRSGKLPTNQRVKWRADSGLS 65

Query: 71  DGKLANRKHTDKDLHGAERRNSPVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAE 130
           DG  AN                 V+L+GGYYDAGDNVK+  PM+FT T LSW AL Y  E
Sbjct: 66  DGASAN-----------------VNLIGGYYDAGDNVKFVWPMSFTTTLLSWAALEYQNE 125

Query: 131 LEAAGEMENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQI 190
           +    ++  L++ IKWGT++ L+A +  + LY +VGD   DH CW RPE+M TPRT+  I
Sbjct: 126 ITFVNQLGYLRSTIKWGTNFILRAHTSTNMLYTQVGDGNSDHSCWERPEDMDTPRTLYSI 185

Query: 191 DSETPGTEIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKGTYDGECPFY 250
            S +PG+E A E +AA+A++S+VF+  + TY+  LLN AK+L++FA  ++G+Y   CPFY
Sbjct: 186 SSSSPGSEAAGEAAAALAAASLVFKLVDSTYSSKLLNNAKSLFEFADKYRGSYQASCPFY 245

Query: 251 CSYSGYNDELLWAATWLYVATRKSVYLKYVL-EESISASVAEFSWDLKYVGAQVLLSK-- 310
           CS+SGY DELLWAA WLY AT +  YL YV+  +  S ++ EFSWD K+ G Q LL+   
Sbjct: 246 CSHSGYQDELLWAAAWLYKATGEKSYLNYVISNKDWSKAINEFSWDNKFAGVQALLASEF 305

Query: 311 -------------ADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYS 370
                         +S++C+ +P S   QI  +PGG++ +RD +N QYVT    +   YS
Sbjct: 306 YNGANDLEKFKTDVESFVCALMPGSSSQQIKPTPGGILFIRDSSNLQYVTTATTILFYYS 365

Query: 371 DILA-AYKQNVKCSDQQFDPAHLMTFAKKQAE 386
             L  A   +++C   QF  + +  FAK Q +
Sbjct: 366 KTLTKAGVGSIQCGSTQFTVSQIRNFAKSQVD 373