Carg23915 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg23915 vs. NCBI nr
Match: KAG7029825.1 (Vacuolar protein sorting-associated protein 41-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1872.1 bits (4848), Expect = 0.0e+00
Identity = 927/927 (100.00%), Postives = 927/927 (100.00%), Query Frame = 0

Query: 1   MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 60
           MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF
Sbjct: 1   MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 60

Query: 61  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 120
           PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT
Sbjct: 61  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 120

Query: 121 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 180
           SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN
Sbjct: 121 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 180

Query: 181 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 240
           DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL
Sbjct: 181 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 240

Query: 241 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 300
           VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP
Sbjct: 241 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 300

Query: 301 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 360
           EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP
Sbjct: 301 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 360

Query: 361 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 420
           KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE
Sbjct: 361 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 420

Query: 421 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 480
           AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL
Sbjct: 421 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 480

Query: 481 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 540
           FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY
Sbjct: 481 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 540

Query: 541 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 600
           ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG
Sbjct: 541 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 600

Query: 601 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 660
           DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE
Sbjct: 601 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 660

Query: 661 ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 720
           ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH
Sbjct: 661 ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 720

Query: 721 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 780
           KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK
Sbjct: 721 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 780

Query: 781 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 840
           ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR
Sbjct: 781 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 840

Query: 841 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 900
           CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF
Sbjct: 841 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 900

Query: 901 EDDDEDDTDAGGPRMRCILCTTAASKS 928
           EDDDEDDTDAGGPRMRCILCTTAASKS
Sbjct: 901 EDDDEDDTDAGGPRMRCILCTTAASKS 927

BLAST of Carg23915 vs. NCBI nr
Match: KAG6586007.1 (Vacuolar protein sorting-associated protein 41-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1872.1 bits (4848), Expect = 0.0e+00
Identity = 927/927 (100.00%), Postives = 927/927 (100.00%), Query Frame = 0

Query: 1    MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 60
            MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF
Sbjct: 103  MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 162

Query: 61   PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 120
            PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT
Sbjct: 163  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 222

Query: 121  SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 180
            SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN
Sbjct: 223  SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 282

Query: 181  DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 240
            DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL
Sbjct: 283  DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 342

Query: 241  VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 300
            VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP
Sbjct: 343  VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 402

Query: 301  EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 360
            EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP
Sbjct: 403  EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 462

Query: 361  KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 420
            KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE
Sbjct: 463  KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 522

Query: 421  AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 480
            AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL
Sbjct: 523  AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 582

Query: 481  FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 540
            FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY
Sbjct: 583  FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 642

Query: 541  ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 600
            ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG
Sbjct: 643  ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 702

Query: 601  DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 660
            DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE
Sbjct: 703  DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 762

Query: 661  ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 720
            ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH
Sbjct: 763  ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 822

Query: 721  KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 780
            KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK
Sbjct: 823  KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 882

Query: 781  ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 840
            ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR
Sbjct: 883  ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 942

Query: 841  CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 900
            CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF
Sbjct: 943  CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 1002

Query: 901  EDDDEDDTDAGGPRMRCILCTTAASKS 928
            EDDDEDDTDAGGPRMRCILCTTAASKS
Sbjct: 1003 EDDDEDDTDAGGPRMRCILCTTAASKS 1029

BLAST of Carg23915 vs. NCBI nr
Match: XP_022938247.1 (vacuolar protein sorting-associated protein 41 homolog [Cucurbita moschata] >XP_022938248.1 vacuolar protein sorting-associated protein 41 homolog [Cucurbita moschata] >XP_022938249.1 vacuolar protein sorting-associated protein 41 homolog [Cucurbita moschata] >XP_022938251.1 vacuolar protein sorting-associated protein 41 homolog [Cucurbita moschata] >XP_022938252.1 vacuolar protein sorting-associated protein 41 homolog [Cucurbita moschata])

HSP 1 Score: 1872.1 bits (4848), Expect = 0.0e+00
Identity = 927/927 (100.00%), Postives = 927/927 (100.00%), Query Frame = 0

Query: 1   MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 60
           MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF
Sbjct: 30  MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 89

Query: 61  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 120
           PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT
Sbjct: 90  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 149

Query: 121 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 180
           SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN
Sbjct: 150 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 209

Query: 181 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 240
           DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL
Sbjct: 210 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 269

Query: 241 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 300
           VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP
Sbjct: 270 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 329

Query: 301 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 360
           EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP
Sbjct: 330 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 389

Query: 361 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 420
           KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE
Sbjct: 390 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 449

Query: 421 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 480
           AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL
Sbjct: 450 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 509

Query: 481 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 540
           FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY
Sbjct: 510 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 569

Query: 541 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 600
           ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG
Sbjct: 570 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 629

Query: 601 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 660
           DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE
Sbjct: 630 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 689

Query: 661 ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 720
           ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH
Sbjct: 690 ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 749

Query: 721 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 780
           KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK
Sbjct: 750 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 809

Query: 781 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 840
           ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR
Sbjct: 810 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 869

Query: 841 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 900
           CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF
Sbjct: 870 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 929

Query: 901 EDDDEDDTDAGGPRMRCILCTTAASKS 928
           EDDDEDDTDAGGPRMRCILCTTAASKS
Sbjct: 930 EDDDEDDTDAGGPRMRCILCTTAASKS 956

BLAST of Carg23915 vs. NCBI nr
Match: XP_023520851.1 (vacuolar protein sorting-associated protein 41 homolog [Cucurbita pepo subsp. pepo] >XP_023520852.1 vacuolar protein sorting-associated protein 41 homolog [Cucurbita pepo subsp. pepo] >XP_023520853.1 vacuolar protein sorting-associated protein 41 homolog [Cucurbita pepo subsp. pepo] >XP_023520854.1 vacuolar protein sorting-associated protein 41 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1864.7 bits (4829), Expect = 0.0e+00
Identity = 921/927 (99.35%), Postives = 926/927 (99.89%), Query Frame = 0

Query: 1   MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 60
           +ADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF
Sbjct: 31  IADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 90

Query: 61  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 120
           PAHTAVVNDLSFD+EGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT
Sbjct: 91  PAHTAVVNDLSFDTEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 150

Query: 121 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 180
           SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN
Sbjct: 151 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 210

Query: 181 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 240
           DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL
Sbjct: 211 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 270

Query: 241 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 300
           VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP
Sbjct: 271 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 330

Query: 301 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 360
           EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP
Sbjct: 331 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 390

Query: 361 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 420
           KD+VIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE
Sbjct: 391 KDIVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 450

Query: 421 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 480
           AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL
Sbjct: 451 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 510

Query: 481 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 540
           FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY
Sbjct: 511 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 570

Query: 541 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 600
           ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG
Sbjct: 571 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 630

Query: 601 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 660
           DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE
Sbjct: 631 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 690

Query: 661 ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 720
           ICI+KNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH
Sbjct: 691 ICIKKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 750

Query: 721 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 780
           KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK
Sbjct: 751 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 810

Query: 781 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 840
           ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENK+FQSIQESVSARMMEVKSKTRGGAR
Sbjct: 811 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKIFQSIQESVSARMMEVKSKTRGGAR 870

Query: 841 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 900
           CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDA KGSGETRHDSTSDFDYDNGEF
Sbjct: 871 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDAKKGSGETRHDSTSDFDYDNGEF 930

Query: 901 EDDDEDDTDAGGPRMRCILCTTAASKS 928
           EDDDEDDTDAGGPRMRCILCTTAASKS
Sbjct: 931 EDDDEDDTDAGGPRMRCILCTTAASKS 957

BLAST of Carg23915 vs. NCBI nr
Match: XP_022969631.1 (vacuolar protein sorting-associated protein 41 homolog [Cucurbita maxima] >XP_022969632.1 vacuolar protein sorting-associated protein 41 homolog [Cucurbita maxima] >XP_022969633.1 vacuolar protein sorting-associated protein 41 homolog [Cucurbita maxima] >XP_022969634.1 vacuolar protein sorting-associated protein 41 homolog [Cucurbita maxima] >XP_022969636.1 vacuolar protein sorting-associated protein 41 homolog [Cucurbita maxima])

HSP 1 Score: 1859.3 bits (4815), Expect = 0.0e+00
Identity = 922/928 (99.35%), Postives = 926/928 (99.78%), Query Frame = 0

Query: 1   MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 60
           MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF
Sbjct: 34  MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 93

Query: 61  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 120
           PAHTAVVNDLSFD+EGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT
Sbjct: 94  PAHTAVVNDLSFDTEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 153

Query: 121 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 180
           SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN
Sbjct: 154 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 213

Query: 181 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 240
           DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL
Sbjct: 214 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 273

Query: 241 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 300
           VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP
Sbjct: 274 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 333

Query: 301 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 360
           EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP
Sbjct: 334 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 393

Query: 361 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 420
           KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE
Sbjct: 394 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 453

Query: 421 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 480
           AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTE+PRLRDTAYEVALVALASNPL
Sbjct: 454 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTEDPRLRDTAYEVALVALASNPL 513

Query: 481 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 540
           FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY
Sbjct: 514 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 573

Query: 541 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 600
           ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG
Sbjct: 574 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 633

Query: 601 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 660
           DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE
Sbjct: 634 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 693

Query: 661 ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 720
           ICI+KNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH
Sbjct: 694 ICIKKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 753

Query: 721 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 780
           KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK
Sbjct: 754 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 813

Query: 781 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 840
           ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR
Sbjct: 814 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 873

Query: 841 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 900
           CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDA KGSGETRHDSTSDFDYDNGEF
Sbjct: 874 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDAKKGSGETRHDSTSDFDYDNGEF 933

Query: 901 EDDD-EDDTDAGGPRMRCILCTTAASKS 928
           +DDD EDDTDAGGPRMRCILCTTAASKS
Sbjct: 934 DDDDYEDDTDAGGPRMRCILCTTAASKS 961

BLAST of Carg23915 vs. ExPASy Swiss-Prot
Match: P93231 (Vacuolar protein sorting-associated protein 41 homolog OS=Solanum lycopersicum OX=4081 GN=VPS41 PE=2 SV=1)

HSP 1 Score: 1512.7 bits (3915), Expect = 0.0e+00
Identity = 738/931 (79.27%), Postives = 829/931 (89.04%), Query Frame = 0

Query: 3   DDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEFPA 62
           ++E EPRLKYQRMG SVPSLL++DAA+C+AVAERMIALGTH G +HILDFLGNQVKEF A
Sbjct: 31  EEEDEPRLKYQRMGASVPSLLSADAATCIAVAERMIALGTHGGAVHILDFLGNQVKEFAA 90

Query: 63  HTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKTSR 122
           HTA VNDL FD++GEYVGSCSDDGSVVINSLFTDE+M+FEYHRPMKAIALDPDYA+ +SR
Sbjct: 91  HTAAVNDLCFDTDGEYVGSCSDDGSVVINSLFTDERMKFEYHRPMKAIALDPDYARTSSR 150

Query: 123 RFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQ 182
           RF  GGLAG LYLN KKWLG++DQVLHSGEGPIHAVKWRTSL+AWAND GVKVYDA+NDQ
Sbjct: 151 RFVTGGLAGQLYLNVKKWLGYRDQVLHSGEGPIHAVKWRTSLVAWANDTGVKVYDASNDQ 210

Query: 183 RITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRLVP 242
           RITFIERPRG PRPELLLP +VWQDD+LLVIGWGTSVKIA IRT Q++ ANGT   + + 
Sbjct: 211 RITFIERPRGIPRPELLLPHIVWQDDSLLVIGWGTSVKIALIRTTQSKGANGTY--KHMS 270

Query: 243 MSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEE-GEKDFSRTAPSRQGNAQRPE 302
           MSS+N+VDIVASFQTSY I+G+APFGD LV+LAYIPGEE GEKDFS T PSRQGNAQRPE
Sbjct: 271 MSSLNQVDIVASFQTSYFISGIAPFGDSLVILAYIPGEEDGEKDFSSTIPSRQGNAQRPE 330

Query: 303 VRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSPK 362
           VR+VTWNNDEL+TDALPVHGFEHYKAKDYSLAHAPF+GSSYAGGQWAAGDEPLYY+VSPK
Sbjct: 331 VRVVTWNNDELATDALPVHGFEHYKAKDYSLAHAPFSGSSYAGGQWAAGDEPLYYIVSPK 390

Query: 363 DVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAEA 422
           DVVIAKPRDAEDHI WLL+HG+HEKALEAVEA QG+SELLDEVGSRYLDHLIVERKYAEA
Sbjct: 391 DVVIAKPRDAEDHINWLLQHGWHEKALEAVEANQGQSELLDEVGSRYLDHLIVERKYAEA 450

Query: 423 ASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPLF 482
           ASLCPKLLRGS SAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALA+NP F
Sbjct: 451 ASLCPKLLRGSASAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALATNPSF 510

Query: 483 HKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLYA 542
           HKDLL+TVKSWPP IYS  PV SAIEPQ NTSSMTD LKEALAELYVIDGQ++K F LYA
Sbjct: 511 HKDLLSTVKSWPPRIYSTTPVFSAIEPQINTSSMTDPLKEALAELYVIDGQHDKAFALYA 570

Query: 543 DLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAGD 602
           DL+KPD+FDFIEK+NLH+A+REKV+QLMM+DCKRAV L IQ ++LIPP+EVVSQL  A D
Sbjct: 571 DLMKPDLFDFIEKHNLHDAVREKVLQLMMIDCKRAVLLLIQQRDLIPPSEVVSQLIAARD 630

Query: 603 KCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHEI 662
           KCD+RYF+HLYLHSLFEVN HAGKD+HD+QVELYADYD KMLL FLRSSQHYTLEKA+EI
Sbjct: 631 KCDYRYFLHLYLHSLFEVNLHAGKDYHDMQVELYADYDPKMLLTFLRSSQHYTLEKAYEI 690

Query: 663 CIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHK 722
           C++K+LL+EQVFILGRMGNAKQAL+VII++LGDIEEA+EFVSMQ DDELWEELI+   HK
Sbjct: 691 CVKKDLLKEQVFILGRMGNAKQALAVIINRLGDIEEAIEFVSMQQDDELWEELIQQSFHK 750

Query: 723 AEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILKA 782
            EMVG+LLEHTVGNLDPLYIVNM+PN LEIPRLRDRLVKI+TDYRTETSLRHGCNDILKA
Sbjct: 751 PEMVGVLLEHTVGNLDPLYIVNMLPNDLEIPRLRDRLVKIVTDYRTETSLRHGCNDILKA 810

Query: 783 DTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGARC 842
           D VNLLVKYY EA+ G+ LS+E D++  +R E  V    + ++S + +EVKSKTRGG RC
Sbjct: 811 DCVNLLVKYYKEAKRGVCLSDEVDDVSSRRGEKSVSHLGERTMSLKSVEVKSKTRGGGRC 870

Query: 843 CICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNG--- 902
           CICFDPFSI N+S+I FFCCHAYH TCL+ES+ ++   K +G     +TS  +Y NG   
Sbjct: 871 CICFDPFSILNVSIIAFFCCHAYHTTCLMESSISVGGKKEAGVAAQRTTSYDEYPNGVND 930

Query: 903 EFEDDD-----EDDTDAGGPRMRCILCTTAA 925
           ++ED+D     E+D  +G   MRCILCTTAA
Sbjct: 931 DYEDEDEEEEEEEDATSGALPMRCILCTTAA 959

BLAST of Carg23915 vs. ExPASy Swiss-Prot
Match: P93043 (Vacuolar protein sorting-associated protein 41 homolog OS=Arabidopsis thaliana OX=3702 GN=VPS41 PE=1 SV=3)

HSP 1 Score: 1436.0 bits (3716), Expect = 0.0e+00
Identity = 713/947 (75.29%), Postives = 815/947 (86.06%), Query Frame = 0

Query: 5   EMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEFPAHT 64
           E EPRLKYQRMGG+VP+LL++DAASC+AVA RMIALGTH GT+ ILD LGNQVKEF AHT
Sbjct: 39  EEEPRLKYQRMGGNVPALLSNDAASCIAVAARMIALGTHDGTVRILDLLGNQVKEFRAHT 98

Query: 65  AVVNDLSFDSEGEYVGSCSDDGSVVINSLFT-DEKMRFEYHRPMKAIALDPDYAKKTSRR 124
           A VND++FD+EGEY+GSCSDDGSVVINSLFT DEKM+F+YHRPMKAI+LDPDY KK S+R
Sbjct: 99  APVNDINFDTEGEYIGSCSDDGSVVINSLFTDDEKMKFDYHRPMKAISLDPDYTKKQSKR 158

Query: 125 FAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQR 184
           F AGGLAGHLY+NSKKW G KDQVLHSGEGPIH+VKWR SLIAWAND GVKVYD A DQR
Sbjct: 159 FVAGGLAGHLYMNSKKWFGNKDQVLHSGEGPIHSVKWRGSLIAWANDVGVKVYDTAKDQR 218

Query: 185 ITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRLVPM 244
           +TFIE+PRGSPRPE LLP LVWQDDTLLVIGWGTSVKIASI+++Q +    T + R + M
Sbjct: 219 VTFIEKPRGSPRPEALLPHLVWQDDTLLVIGWGTSVKIASIKSDQQQ----TGTFRQIQM 278

Query: 245 SSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGE-EGEKDFSRTAP-SRQGNAQRPE 304
           SS+ +VDIVASFQTSY I+G+APFGD LV+LAYIP E +GEK+FS T   SRQGNAQRPE
Sbjct: 279 SSLTQVDIVASFQTSYYISGIAPFGDSLVILAYIPIEGDGEKEFSSTTTLSRQGNAQRPE 338

Query: 305 VRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSPK 364
           +RIV+WNNDEL+ DALPVHGFEHYKAKDYSLAHAPF GSSYAGGQWAAGDEPLYY+VSPK
Sbjct: 339 IRIVSWNNDELTMDALPVHGFEHYKAKDYSLAHAPFPGSSYAGGQWAAGDEPLYYIVSPK 398

Query: 365 DVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAEA 424
           DVVIAKPRDAEDHI WLL+HGFHEKAL AVEA +GR+EL+D+VG+ YLDHLIVERKYAEA
Sbjct: 399 DVVIAKPRDAEDHINWLLQHGFHEKALAAVEASEGRTELIDKVGAGYLDHLIVERKYAEA 458

Query: 425 ASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPLF 484
           ASLCPKLLRGS SAWERWVFHFA LRQLPVLVPY+PT+NPRL+DT YEVALVALA+NP +
Sbjct: 459 ASLCPKLLRGSASAWERWVFHFAQLRQLPVLVPYMPTDNPRLKDTVYEVALVALATNPSY 518

Query: 485 HKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLYA 544
           HK+LL+ VKSWP  +YSAL VISAIEPQ NTSSMTDALKEALAELYVIDGQY+K F LYA
Sbjct: 519 HKELLSAVKSWPRSVYSALTVISAIEPQLNTSSMTDALKEALAELYVIDGQYQKAFSLYA 578

Query: 545 DLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQL----- 604
           DLLKP++FDFIEKY+LHEAIR KVVQLM+LDCKRA  LFIQN++LIPP+EVV QL     
Sbjct: 579 DLLKPEVFDFIEKYSLHEAIRGKVVQLMLLDCKRATVLFIQNRDLIPPSEVVPQLLKAGK 638

Query: 605 ----FKAGDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQH 664
                KAG KCD RY+++LYLH+LFEV+   GKDFHD+QVELYA+YD KMLLPFLRSSQH
Sbjct: 639 NPQVLKAGKKCDSRYYLYLYLHALFEVSHDTGKDFHDMQVELYAEYDTKMLLPFLRSSQH 698

Query: 665 YTLEKAHEICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWE 724
           Y LEKA+E+C++K+ LREQVF+LGRMGNAKQAL+VII+KLGDIEEAVEFVSMQHDD+LWE
Sbjct: 699 YKLEKAYELCVKKDFLREQVFVLGRMGNAKQALAVIINKLGDIEEAVEFVSMQHDDDLWE 758

Query: 725 ELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLR 784
           ELIK CL+K EMVG+LLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKI+TDYRTETSLR
Sbjct: 759 ELIKQCLNKPEMVGLLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIVTDYRTETSLR 818

Query: 785 HGCNDILKADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSEN-----KVFQSIQESVSAR 844
           HGCNDILK D VNLLVK +NEAR G+ LS+E+D+ R KR +N          + +S+S +
Sbjct: 819 HGCNDILKTDIVNLLVKCFNEARRGVCLSHEDDDSRAKREDNNRSSFSQRMVVDKSLSIK 878

Query: 845 MMEVKSKTRGGARCCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRH 904
           M EVKSKTRG  RCC+CFDP SI+  +V+VFFCCHAYHETCL+++     +N     T+ 
Sbjct: 879 MTEVKSKTRGDTRCCMCFDPVSIRGDTVVVFFCCHAYHETCLMDAAF---SNNNHKTTKG 938

Query: 905 DSTSDFDYDNG--EFEDDDEDDTDAGG-----PRMRCILCTTAASKS 928
            S  ++ YDNG  E E+D+E+D D  G      R+RCILCTTAA+ S
Sbjct: 939 SSGYEYSYDNGVDEEEEDEEEDEDGDGDRPGRSRLRCILCTTAAAAS 978

BLAST of Carg23915 vs. ExPASy Swiss-Prot
Match: Q5KU39 (Vacuolar protein sorting-associated protein 41 homolog OS=Mus musculus OX=10090 GN=Vps41 PE=1 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.3e-158
Identity = 320/885 (36.16%), Postives = 492/885 (55.59%), Query Frame = 0

Query: 3   DDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEFPA 62
           + E EP+LKY+R+   V  +L  DAASC+ V ++ +ALGTH G +++LD  GN  ++F  
Sbjct: 22  ESEEEPKLKYERLSNGVTEILQKDAASCMTVHDKFLALGTHYGKVYLLDVQGNITQKFDV 81

Query: 63  HTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKTSR 122
               +N +S D  GE++G CS+DG + +  L++ E+    +  P+K IA+ P + + + +
Sbjct: 82  SPVKINQISLDDSGEHMGVCSEDGKLQVFGLYSGEEFHETFDCPIKIIAVHPQFVRSSCK 141

Query: 123 RFAAGGLAGHLYLNSKKWLG-FKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAND 182
           +F  GG    L L  + W+  +K  VLH GEG I +VKWR  LIAWAN+ GVKV+D  + 
Sbjct: 142 QFVTGG--KKLLLFERTWMNRWKSSVLHEGEGNIRSVKWRGHLIAWANNMGVKVFDITSK 201

Query: 183 QRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRLV 242
           QRI+ + R   S RP++    L W+D+  L+IGWGTS+KI S++           +S + 
Sbjct: 202 QRISNVPRDDISLRPDMYPCSLCWKDNVTLIIGWGTSIKICSVKER--------HASEMR 261

Query: 243 PMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIP--GEEGEKDFSRTAPSRQGNAQR 302
            + S   V+IV+ F+T + I+G+AP  D LVVL+Y+    E+ E+++            R
Sbjct: 262 DLPS-RYVEIVSQFETEFYISGLAPLCDQLVVLSYVKEVSEKTEREY----------CAR 321

Query: 303 PEVRIV---TWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYY 362
           P + I+       +E+S+DAL V GF+  + +DY L         Y+ G      E L+Y
Sbjct: 322 PRLDIIQPLPETCEEISSDALTVRGFQENECRDYHL--------EYSEG------ESLFY 381

Query: 363 VVSPKDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQ---GRSELLDEVGSRYLDHLI 422
           VVSP+DVV+AK RD +DHI WLLE   +E+AL A E  Q    R ++LD +G  Y++HL+
Sbjct: 382 VVSPRDVVVAKERDQDDHIDWLLEKKKYEEALMAAEISQRNIKRHKILD-IGLAYVNHLV 441

Query: 423 VERKYAEAASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALV 482
              +Y  AA  C K+L  + S WE  V+ F  + QL  + PY+P  +P L+   YE+ L 
Sbjct: 442 ERGEYDMAARKCQKILGKNASLWEYEVYKFKEIGQLKAISPYLPRGDPVLKPLIYEMILH 501

Query: 483 ALASNPLFHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQY 542
               +   ++   T ++ WP  +Y+   ++ A+       S    L + LAELY  D  Y
Sbjct: 502 EFLESD--YEGFATLIREWPGDLYNNSVIVQAVRDHLKKDSQNKTLLKTLAELYTYDKNY 561

Query: 543 EKGFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVV 602
                +Y  L   D+F  I K+NL  +I++K+V LM  D ++AV + + N++ I   +VV
Sbjct: 562 GNALEIYLTLRHKDVFQLIHKHNLFSSIKDKIVLLMDFDSEKAVDMLLDNEDKISIKKVV 621

Query: 603 SQLFKAGDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHY 662
            +L       D     H+YLH LF+ + H G+ +H+ Q+ LYA+YD   LLPFLR S H 
Sbjct: 622 EELE------DRPELQHVYLHKLFKRDHHKGQRYHEKQISLYAEYDRPNLLPFLRDSTHC 681

Query: 663 TLEKAHEICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEE 722
            LEKA EIC Q+N + E V++L RMGN++ AL +I+++L D+++A+EF   Q D ELWE+
Sbjct: 682 PLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDKAIEFAKEQDDGELWED 741

Query: 723 LIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRH 782
           LI   + K   +  LL +   ++DP+ +++ +  G+EIP LRD LVKI+ DY  +  LR 
Sbjct: 742 LILYSIDKPPFITGLLNNIGTHVDPILLIHRIKEGMEIPNLRDSLVKILQDYNLQILLRE 801

Query: 783 GCNDILKADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKS 842
           GC  IL AD+++LL K +     G+ +  E               +I ES  + ++   +
Sbjct: 802 GCKKILVADSLSLLKKMHRTQMKGVLVDEE---------------NICESCLSPILPTDA 831

Query: 843 KTRGGARCCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDA 879
                        PF     SV+VF C H +H+ CL   + N  A
Sbjct: 862 A-----------KPF-----SVVVFHCRHMFHKECLPMPSMNAPA 831

BLAST of Carg23915 vs. ExPASy Swiss-Prot
Match: P49754 (Vacuolar protein sorting-associated protein 41 homolog OS=Homo sapiens OX=9606 GN=VPS41 PE=1 SV=3)

HSP 1 Score: 560.1 bits (1442), Expect = 4.8e-158
Identity = 315/880 (35.80%), Postives = 484/880 (55.00%), Query Frame = 0

Query: 3   DDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEFPA 62
           + E EP+LKY+R+   V  +L  DAASC+ V ++ +ALGTH G +++LD  GN  ++F  
Sbjct: 23  ESEEEPKLKYERLSNGVTEILQKDAASCMTVHDKFLALGTHYGKVYLLDVQGNITQKFDV 82

Query: 63  HTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKTSR 122
               +N +S D  GE++G CS+DG V +  L++ E+    +  P+K IA+ P + + + +
Sbjct: 83  SPVKINQISLDESGEHMGVCSEDGKVQVFGLYSGEEFHETFDCPIKIIAVHPHFVRSSCK 142

Query: 123 RFAAGGLAGHLYLNSKKWLG-FKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAND 182
           +F  GG    L L  + W+  +K  VLH GEG I +VKWR  LIAWAN+ GVK++D  + 
Sbjct: 143 QFVTGG--KKLLLFERSWMNRWKSAVLHEGEGNIRSVKWRGHLIAWANNMGVKIFDIISK 202

Query: 183 QRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRLV 242
           QRIT + R   S RP++    L W+D+  L+IGWGTSVK+ S++           +S + 
Sbjct: 203 QRITNVPRDDISLRPDMYPCSLCWKDNVTLIIGWGTSVKVCSVKER--------HASEMR 262

Query: 243 PMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIP--GEEGEKDFSRTAPSRQGNAQR 302
            + S   V+IV+ F+T + I+G+AP  D LVVL+Y+    E+ E+++            R
Sbjct: 263 DLPS-RYVEIVSQFETEFYISGLAPLCDQLVVLSYVKEISEKTEREY----------CAR 322

Query: 303 PEVRIV---TWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYY 362
           P + I+   +   +E+S+DAL V GF+  + +DY L         Y+ G      E L+Y
Sbjct: 323 PRLDIIQPLSETCEEISSDALTVRGFQENECRDYHL--------EYSEG------ESLFY 382

Query: 363 VVSPKDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQ---GRSELLDEVGSRYLDHLI 422
           +VSP+DVV+AK RD +DHI WLLE   +E+AL A E  Q    R ++LD +G  Y++HL+
Sbjct: 383 IVSPRDVVVAKERDQDDHIDWLLEKKKYEEALMAAEISQKNIKRHKILD-IGLAYINHLV 442

Query: 423 VERKYAEAASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALV 482
               Y  AA  C K+L  + + WE  V+ F  + QL  + PY+P  +P L+   YE+ L 
Sbjct: 443 ERGDYDIAARKCQKILGKNAALWEYEVYKFKEIGQLKAISPYLPRGDPVLKPLIYEMILH 502

Query: 483 ALASNPLFHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQY 542
               +   ++   T ++ WP  +Y+   ++ A+       S    L + LAELY  D  Y
Sbjct: 503 EFLESD--YEGFATLIREWPGDLYNNSVIVQAVRDHLKKDSQNKTLLKTLAELYTYDKNY 562

Query: 543 EKGFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVV 602
                +Y  L   D+F  I K+NL  +I++K+V LM  D ++AV + + N++ I   +VV
Sbjct: 563 GNALEIYLTLRHKDVFQLIHKHNLFSSIKDKIVLLMDFDSEKAVDMLLDNEDKISIKKVV 622

Query: 603 SQLFKAGDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHY 662
            +L       D     H+YLH LF+ + H G+ +H+ Q+ LYA+YD   LLPFLR S H 
Sbjct: 623 EELE------DRPELQHVYLHKLFKRDHHKGQRYHEKQISLYAEYDRPNLLPFLRDSTHC 682

Query: 663 TLEKAHEICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEE 722
            LEKA EIC Q+N + E V++L RMGN++ AL +I+++L D+++A+EF   Q D ELWE+
Sbjct: 683 PLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDKAIEFAKEQDDGELWED 742

Query: 723 LIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRH 782
           LI   + K   +  LL +   ++DP+ +++ +  G+EIP LRD LVKI+ DY  +  LR 
Sbjct: 743 LILYSIDKPPFITGLLNNIGTHVDPILLIHRIKEGMEIPNLRDSLVKILQDYNLQILLRE 802

Query: 783 GCNDILKADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKS 842
           GC  IL AD+++LL K +     G+ +  E                              
Sbjct: 803 GCKKILVADSLSLLKKMHRTQMKGVLVDEEN----------------------------- 823

Query: 843 KTRGGARCCICFDPF----SIQNMSVIVFFCCHAYHETCL 870
                  C  C  P     + +  SV+VF C H +H+ CL
Sbjct: 863 ------ICESCLSPILPSDAAKPFSVVVFHCRHMFHKECL 823

BLAST of Carg23915 vs. ExPASy Swiss-Prot
Match: Q9P7N3 (Vacuolar protein sorting-associated protein 41 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vps41 PE=3 SV=2)

HSP 1 Score: 415.6 bits (1067), Expect = 1.5e-114
Identity = 243/809 (30.04%), Postives = 431/809 (53.28%), Query Frame = 0

Query: 2   ADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEFP 61
           +D++ EP+L Y+R+         +D  S  A+++     G+H G I+I    G  +++  
Sbjct: 18  SDEDEEPKLIYERITEKFQGCFMNDTISACAISKEHFFFGSHNGAIYIYQKNGILLRKMI 77

Query: 62  AHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKTS 121
            H+A V DLS D E E + SCS DG ++I+++ T E    ++ RP+ ++A+DP Y+ ++S
Sbjct: 78  LHSASVVDLSVDLESENLASCSMDGKMIISNITTRETTVHDFKRPLLSVAIDPYYSTRSS 137

Query: 122 RRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAND 181
           R+  +GG AG + L+ K WLG KD VL +  G ++ + W T+ IAWA+D G+ VY     
Sbjct: 138 RQVLSGGRAGKVVLSEKGWLGNKDTVLQADCGAVYKISWYTTYIAWASDLGITVYSTEFG 197

Query: 182 QRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRLV 241
           + +  +E P+  P  E+   QL WQ ++ LVIGW   + I SI+  ++  AN        
Sbjct: 198 KVLGRLEPPKRLPNDEIFPYQLFWQSESRLVIGWSDQIMIVSIQ--RSNVAN-------- 257

Query: 242 PMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRPE 301
               + ++ + A  +   +++G+   G  ++ LAYI   E   DF+   PS++    RPE
Sbjct: 258 ---ELPKISLQALLEIDSIVSGVLMLGFNILTLAYIANVE---DFTSAIPSQRIEGCRPE 317

Query: 302 VRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSPK 361
           +R++  +  EL  DA+ +  +   +  DY L   P + S               +V+SP 
Sbjct: 318 LRLIDSSFKELCGDAIGLANYSRLQPSDYHLLPDPSSNSH-------------SFVISPN 377

Query: 362 DVVIAKPRDAEDHIAWLLEHGFHEKALEAVE--AGQGRSELLDEVGSRYLDHLIVERKYA 421
           D+V  + R+  DH+ +L+    + +A++AV+       S  + E+  +Y+ HL+ + +Y 
Sbjct: 378 DIVYVRERNQIDHVKYLVSKEMYAEAIDAVKKLPEIPPSLQISELAKKYIFHLLGKGQYK 437

Query: 422 EAASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVA-LASN 481
           EA  + P L   +++ WE+WVF FA    L  +  ++PT    L    YE+ L   LA++
Sbjct: 438 EAGMVIPSLYNDNLAEWEQWVFVFAENDHLEDIADFLPTGENHLSPLVYEMILAQYLATD 497

Query: 482 P-LFHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGF 541
              F+K L      WP ++YS   + +A   +F  +  +  L E+LA LY+ D      F
Sbjct: 498 ERTFNKKL----HEWPTMLYSVSTIRNATLKKFKENQKSSTLTESLAFLYLEDNMPIDAF 557

Query: 542 LLYADLLKPDIFDFIEKYNLHEAIREKVVQLMML-------DCKRAV-PLFIQNKELIPP 601
            LY  L      D I ++NL++  R  V+ LM++       D K A+  + +Q+    PP
Sbjct: 558 HLYLKLHSELCIDLILQHNLYDEARASVLLLMLISSKGKSSDTKSAMSSMLVQHVHSFPP 617

Query: 602 NEVVSQLFKAGDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRS 661
            EV+ Q+           F++ Y      + P++  ++ D++++++A++D K    FL +
Sbjct: 618 QEVIMQIHSVPQ------FLYEYFCEFELMYPNSLMEYGDLKLDVFAEFDRKRFFDFLVN 677

Query: 662 SQHYTLEKAHEICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDE 721
           +Q Y+L+ A +IC Q N L E V+ILGRMGN K+AL +II++L DI  A+ +V  Q D E
Sbjct: 678 TQCYSLDHAAQICKQYNYLDELVYILGRMGNNKEALMLIINELLDIGRAIRYVKEQADRE 737

Query: 722 LWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTET 781
           LW++LI   L K E +  LLE+   + +   +++ +P G ++P ++  + K++ D++++ 
Sbjct: 738 LWDDLISYSLDKPEFICTLLENIGTDENARNLLSKIPPGTKLPHMKKSISKLLADHQSQV 787

Query: 782 SLRHGCNDILKADTVNLLVKYYNEARHGI 799
            L   C  + K +++++ +KY  + + G+
Sbjct: 798 QLYQSCYKLFKNESISMAIKYREQEQSGL 787

BLAST of Carg23915 vs. ExPASy TrEMBL
Match: A0A6J1FDJ3 (Vacuolar protein sorting-associated protein 41 homolog OS=Cucurbita moschata OX=3662 GN=LOC111444390 PE=3 SV=1)

HSP 1 Score: 1872.1 bits (4848), Expect = 0.0e+00
Identity = 927/927 (100.00%), Postives = 927/927 (100.00%), Query Frame = 0

Query: 1   MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 60
           MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF
Sbjct: 30  MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 89

Query: 61  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 120
           PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT
Sbjct: 90  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 149

Query: 121 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 180
           SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN
Sbjct: 150 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 209

Query: 181 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 240
           DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL
Sbjct: 210 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 269

Query: 241 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 300
           VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP
Sbjct: 270 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 329

Query: 301 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 360
           EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP
Sbjct: 330 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 389

Query: 361 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 420
           KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE
Sbjct: 390 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 449

Query: 421 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 480
           AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL
Sbjct: 450 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 509

Query: 481 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 540
           FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY
Sbjct: 510 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 569

Query: 541 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 600
           ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG
Sbjct: 570 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 629

Query: 601 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 660
           DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE
Sbjct: 630 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 689

Query: 661 ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 720
           ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH
Sbjct: 690 ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 749

Query: 721 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 780
           KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK
Sbjct: 750 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 809

Query: 781 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 840
           ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR
Sbjct: 810 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 869

Query: 841 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 900
           CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF
Sbjct: 870 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 929

Query: 901 EDDDEDDTDAGGPRMRCILCTTAASKS 928
           EDDDEDDTDAGGPRMRCILCTTAASKS
Sbjct: 930 EDDDEDDTDAGGPRMRCILCTTAASKS 956

BLAST of Carg23915 vs. ExPASy TrEMBL
Match: A0A6J1I1J7 (Vacuolar protein sorting-associated protein 41 homolog OS=Cucurbita maxima OX=3661 GN=LOC111468604 PE=3 SV=1)

HSP 1 Score: 1859.3 bits (4815), Expect = 0.0e+00
Identity = 922/928 (99.35%), Postives = 926/928 (99.78%), Query Frame = 0

Query: 1   MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 60
           MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF
Sbjct: 34  MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 93

Query: 61  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 120
           PAHTAVVNDLSFD+EGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT
Sbjct: 94  PAHTAVVNDLSFDTEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 153

Query: 121 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 180
           SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN
Sbjct: 154 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 213

Query: 181 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 240
           DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL
Sbjct: 214 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 273

Query: 241 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 300
           VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP
Sbjct: 274 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 333

Query: 301 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 360
           EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP
Sbjct: 334 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 393

Query: 361 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 420
           KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE
Sbjct: 394 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 453

Query: 421 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 480
           AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTE+PRLRDTAYEVALVALASNPL
Sbjct: 454 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTEDPRLRDTAYEVALVALASNPL 513

Query: 481 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 540
           FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY
Sbjct: 514 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 573

Query: 541 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 600
           ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG
Sbjct: 574 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 633

Query: 601 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 660
           DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE
Sbjct: 634 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 693

Query: 661 ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 720
           ICI+KNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH
Sbjct: 694 ICIKKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 753

Query: 721 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 780
           KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK
Sbjct: 754 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 813

Query: 781 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 840
           ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR
Sbjct: 814 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 873

Query: 841 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 900
           CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDA KGSGETRHDSTSDFDYDNGEF
Sbjct: 874 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDAKKGSGETRHDSTSDFDYDNGEF 933

Query: 901 EDDD-EDDTDAGGPRMRCILCTTAASKS 928
           +DDD EDDTDAGGPRMRCILCTTAASKS
Sbjct: 934 DDDDYEDDTDAGGPRMRCILCTTAASKS 961

BLAST of Carg23915 vs. ExPASy TrEMBL
Match: A0A0A0LY23 (Vacuolar protein sorting-associated protein 41 homolog OS=Cucumis sativus OX=3659 GN=Csa_1G144290 PE=3 SV=1)

HSP 1 Score: 1778.1 bits (4604), Expect = 0.0e+00
Identity = 877/929 (94.40%), Postives = 902/929 (97.09%), Query Frame = 0

Query: 1   MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 60
           +ADDE EPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGT+HILDFLGNQVKEF
Sbjct: 32  IADDEEEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTVHILDFLGNQVKEF 91

Query: 61  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 120
           PAHTAVVNDLSFD+EGEYVGSCSDDGSVVINSLFTDE+MRFEYHRPMKAIALDPDYAKKT
Sbjct: 92  PAHTAVVNDLSFDTEGEYVGSCSDDGSVVINSLFTDERMRFEYHRPMKAIALDPDYAKKT 151

Query: 121 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 180
           SRRFAAGGLAGHLY NSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN
Sbjct: 152 SRRFAAGGLAGHLYFNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 211

Query: 181 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 240
           DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTN NRAANGTQSSR 
Sbjct: 212 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNHNRAANGTQSSRH 271

Query: 241 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 300
           VP SSMNRVDIVASFQTSYLITGMAPFGD LVVLAYIPGEEGEKDFS TAPSRQGNAQRP
Sbjct: 272 VPTSSMNRVDIVASFQTSYLITGMAPFGDVLVVLAYIPGEEGEKDFSMTAPSRQGNAQRP 331

Query: 301 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 360
           EVR+VTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAG EPLYY+VSP
Sbjct: 332 EVRVVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGVEPLYYIVSP 391

Query: 361 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 420
           KD+VIAKPRDAEDHIAWLLEHG+HEKALEAVEAGQGRSELLDEVGS+YLDHLIVERKYAE
Sbjct: 392 KDIVIAKPRDAEDHIAWLLEHGWHEKALEAVEAGQGRSELLDEVGSKYLDHLIVERKYAE 451

Query: 421 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 480
           AA LCPKLLRGS SAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASN  
Sbjct: 452 AALLCPKLLRGSASAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNSS 511

Query: 481 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 540
           FHKDLLTTVK+WPPVIYSA+PVISAIEPQFNTSSMTDALKEALAELYVIDGQYEK FLLY
Sbjct: 512 FHKDLLTTVKTWPPVIYSAVPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLY 571

Query: 541 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 600
           ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAV LFIQNKELIPPNEVVSQLFKAG
Sbjct: 572 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVQLFIQNKELIPPNEVVSQLFKAG 631

Query: 601 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 660
           DKCDFRYF+HLYLHSLFEVNPHAGKDFHDIQVELYADYD KMLLPFLRSSQHYTLEKA++
Sbjct: 632 DKCDFRYFLHLYLHSLFEVNPHAGKDFHDIQVELYADYDTKMLLPFLRSSQHYTLEKAYD 691

Query: 661 ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 720
           ICI+KNLLREQVFILGRMGNAKQAL+VIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH
Sbjct: 692 ICIKKNLLREQVFILGRMGNAKQALAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 751

Query: 721 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 780
           KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK
Sbjct: 752 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 811

Query: 781 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 840
           ADTVNLLVKYY EARHGIYLSNEEDE RGKR+ENKV QSIQ+S++ RMMEVKSKTRGG R
Sbjct: 812 ADTVNLLVKYYKEARHGIYLSNEEDEARGKRNENKVSQSIQKSLNVRMMEVKSKTRGGTR 871

Query: 841 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 900
           CCICF+PFSIQN+SVIVFFCCHAYHETCLIESTSNLDA KG+GET HD TSDFDYDNGE 
Sbjct: 872 CCICFNPFSIQNISVIVFFCCHAYHETCLIESTSNLDAMKGTGETGHDLTSDFDYDNGEI 931

Query: 901 EDDD--EDDTDAGGPRMRCILCTTAASKS 928
           EDD+  EDDTD GGPRMRCILCTTAASK+
Sbjct: 932 EDDEDGEDDTDVGGPRMRCILCTTAASKT 960

BLAST of Carg23915 vs. ExPASy TrEMBL
Match: A0A1S3CGC3 (Vacuolar protein sorting-associated protein 41 homolog OS=Cucumis melo OX=3656 GN=LOC103500583 PE=3 SV=1)

HSP 1 Score: 1776.9 bits (4601), Expect = 0.0e+00
Identity = 875/929 (94.19%), Postives = 902/929 (97.09%), Query Frame = 0

Query: 1   MADDEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEF 60
           MADDE EPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGT+HILDFLGNQVKEF
Sbjct: 32  MADDEEEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTVHILDFLGNQVKEF 91

Query: 61  PAHTAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKT 120
           PAHTAVVNDLSFD+EGEYVGSCSDDGSVVINSLFTDE+MRFEYHRPMKAIALDPDYAKKT
Sbjct: 92  PAHTAVVNDLSFDTEGEYVGSCSDDGSVVINSLFTDERMRFEYHRPMKAIALDPDYAKKT 151

Query: 121 SRRFAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 180
           SRRFAAGGLAGHLY NSKKWLG+KDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN
Sbjct: 152 SRRFAAGGLAGHLYFNSKKWLGYKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAAN 211

Query: 181 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRL 240
           DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTN NRAANGTQSSR 
Sbjct: 212 DQRITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNHNRAANGTQSSRH 271

Query: 241 VPMSSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRP 300
           VP SSMNRVDIVASFQTSYLITGMAPFGD LVVLAYIPGEEGEKDFS TAPSRQGNAQRP
Sbjct: 272 VPTSSMNRVDIVASFQTSYLITGMAPFGDVLVVLAYIPGEEGEKDFSITAPSRQGNAQRP 331

Query: 301 EVRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSP 360
           EVR+VTWNNDELSTDA PVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAG EPLYY+VSP
Sbjct: 332 EVRVVTWNNDELSTDARPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGVEPLYYIVSP 391

Query: 361 KDVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAE 420
           KD+VIAKPRDAEDHIAWLLEHG+HEKALEAVEAGQGRSELLDEVGS+YLDHLIVERKYAE
Sbjct: 392 KDIVIAKPRDAEDHIAWLLEHGWHEKALEAVEAGQGRSELLDEVGSKYLDHLIVERKYAE 451

Query: 421 AASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPL 480
           AASLCPKLLRGS SAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASN L
Sbjct: 452 AASLCPKLLRGSASAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNSL 511

Query: 481 FHKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLY 540
           FHKDLLTTVK+WPPVIYSA+PVISAIEPQFNTSSMTDALKEALAELYV+DGQYEK FLLY
Sbjct: 512 FHKDLLTTVKTWPPVIYSAVPVISAIEPQFNTSSMTDALKEALAELYVVDGQYEKAFLLY 571

Query: 541 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAG 600
           ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAV LFIQNKELIPPNEVVSQLFKAG
Sbjct: 572 ADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVQLFIQNKELIPPNEVVSQLFKAG 631

Query: 601 DKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHE 660
           DKCDFRYF+HLYLHSLFEVNPHAGKDFHDIQVELYADYD KMLLPFLRSSQHYTLEKA++
Sbjct: 632 DKCDFRYFLHLYLHSLFEVNPHAGKDFHDIQVELYADYDTKMLLPFLRSSQHYTLEKAYD 691

Query: 661 ICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLH 720
           ICI+KNLLREQVFILGRMGNAKQAL+VIIDKLGDIEEAVEFVSMQHDD+LWEELIKLCLH
Sbjct: 692 ICIKKNLLREQVFILGRMGNAKQALAVIIDKLGDIEEAVEFVSMQHDDDLWEELIKLCLH 751

Query: 721 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 780
           KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK
Sbjct: 752 KAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILK 811

Query: 781 ADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGAR 840
           ADTVNLLVKYY EARHGIYLSNEEDE RGKR+ENKV QSIQ+S++ RMMEVKSKTRGG R
Sbjct: 812 ADTVNLLVKYYKEARHGIYLSNEEDEARGKRNENKVSQSIQKSLNVRMMEVKSKTRGGTR 871

Query: 841 CCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEF 900
           CCICF+PFSIQN+SVIVFFCCHAYHETCLIESTSNLDA KG+GET HD  SDFDYDNGE 
Sbjct: 872 CCICFNPFSIQNISVIVFFCCHAYHETCLIESTSNLDAKKGTGETGHDLASDFDYDNGEM 931

Query: 901 EDDD--EDDTDAGGPRMRCILCTTAASKS 928
           EDD+  EDDTD GGPRMRCILCTTAASK+
Sbjct: 932 EDDEDGEDDTDMGGPRMRCILCTTAASKT 960

BLAST of Carg23915 vs. ExPASy TrEMBL
Match: A0A6J1DHI3 (Vacuolar protein sorting-associated protein 41 homolog OS=Momordica charantia OX=3673 GN=LOC111020519 PE=3 SV=1)

HSP 1 Score: 1764.2 bits (4568), Expect = 0.0e+00
Identity = 870/926 (93.95%), Postives = 890/926 (96.11%), Query Frame = 0

Query: 4   DEMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEFPAH 63
           DE EPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGT+HILDFLGNQVKEF AH
Sbjct: 35  DEEEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTVHILDFLGNQVKEFSAH 94

Query: 64  TAVVNDLSFDSEGEYVGSCSDDGSVVINSLFTDEKMRFEYHRPMKAIALDPDYAKKTSRR 123
           TAVVNDLSFD EGEYVGSCSDDGSVVINSLFTDE+MRFEYHRPMKAIALDPDY KKTSRR
Sbjct: 95  TAVVNDLSFDIEGEYVGSCSDDGSVVINSLFTDERMRFEYHRPMKAIALDPDYVKKTSRR 154

Query: 124 FAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQR 183
           FAAGGLAGHLY NSKKWLG+KDQVLHSGEGPIH VKWRTSLIAWANDAGVKVYDAANDQR
Sbjct: 155 FAAGGLAGHLYFNSKKWLGYKDQVLHSGEGPIHTVKWRTSLIAWANDAGVKVYDAANDQR 214

Query: 184 ITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRLVPM 243
           ITFIERPRGSPRPELLLPQLVWQDDT+LVIGWGTSVKIASIRTNQNRAANGTQSSR VP+
Sbjct: 215 ITFIERPRGSPRPELLLPQLVWQDDTMLVIGWGTSVKIASIRTNQNRAANGTQSSRHVPL 274

Query: 244 SSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRTAPSRQGNAQRPEVR 303
           SSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSR+APSRQGNAQRPEVR
Sbjct: 275 SSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGEEGEKDFSRSAPSRQGNAQRPEVR 334

Query: 304 IVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSPKDV 363
           +VTWNNDELSTDALPVHGFEHYKAKDYSLAHAPF+GSSYAGGQWAAGDEPLYY+VSPKDV
Sbjct: 335 MVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFSGSSYAGGQWAAGDEPLYYIVSPKDV 394

Query: 364 VIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAEAAS 423
           VIAKPRDAEDHIAWLLEHG+HEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAEAAS
Sbjct: 395 VIAKPRDAEDHIAWLLEHGWHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAEAAS 454

Query: 424 LCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPLFHK 483
           LCPKLLRGS SAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVAL SNP FHK
Sbjct: 455 LCPKLLRGSASAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALVSNPSFHK 514

Query: 484 DLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLYADL 543
           DLL TVKSWPPVIYSALPVISAIEPQ NTSSMTDALKEALAELYVIDGQYEK FLLYADL
Sbjct: 515 DLLATVKSWPPVIYSALPVISAIEPQCNTSSMTDALKEALAELYVIDGQYEKAFLLYADL 574

Query: 544 LKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQLFKAGDKC 603
           LKPDIFDFIEKYNLHE IREKVVQLMMLDCKRAVPLFIQNKELIPPNEV+SQL KA D C
Sbjct: 575 LKPDIFDFIEKYNLHETIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVISQLLKARDTC 634

Query: 604 DFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAHEICI 663
           DFRYFMHLYLHSLFE NPHAGKDFHDIQVELYADYD KMLLPFLRSSQHYTLEKA+EICI
Sbjct: 635 DFRYFMHLYLHSLFEANPHAGKDFHDIQVELYADYDRKMLLPFLRSSQHYTLEKAYEICI 694

Query: 664 QKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAE 723
           +KNLLREQVFILGRMGNAKQ L+VIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAE
Sbjct: 695 KKNLLREQVFILGRMGNAKQVLAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAE 754

Query: 724 MVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILKADT 783
           MVGMLLEHTVGNLDPLYIVN VPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILKADT
Sbjct: 755 MVGMLLEHTVGNLDPLYIVNRVPNGLEIPRLRDRLVKIITDYRTETSLRHGCNDILKADT 814

Query: 784 VNLLVKYYNEARHGIYLSNEEDEIRGKRSENKVFQSIQESVSARMMEVKSKTRGGARCCI 843
           VNLLVKYY EA+HGIYLSNEEDE RGKR+ENKV Q+IQ+S S RMMEVKSKTRGG RCCI
Sbjct: 815 VNLLVKYYKEAKHGIYLSNEEDEARGKRNENKVSQTIQKSSSVRMMEVKSKTRGGTRCCI 874

Query: 844 CFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRHDSTSDFDYDNGEFEDD 903
           CFDPFSIQN+SVIVFFCCHAYH TCL ESTSNLDA KG+GETRHDSTSDFDYDNGE EDD
Sbjct: 875 CFDPFSIQNISVIVFFCCHAYHATCLTESTSNLDAKKGAGETRHDSTSDFDYDNGEVEDD 934

Query: 904 DED----DTDAGGPRMRCILCTTAAS 926
           DED    DTD GGPRMRCILCTTAAS
Sbjct: 935 DEDDDENDTDVGGPRMRCILCTTAAS 960

BLAST of Carg23915 vs. TAIR 10
Match: AT1G08190.1 (vacuolar protein sorting 41 )

HSP 1 Score: 1436.0 bits (3716), Expect = 0.0e+00
Identity = 713/947 (75.29%), Postives = 815/947 (86.06%), Query Frame = 0

Query: 5   EMEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTIHILDFLGNQVKEFPAHT 64
           E EPRLKYQRMGG+VP+LL++DAASC+AVA RMIALGTH GT+ ILD LGNQVKEF AHT
Sbjct: 39  EEEPRLKYQRMGGNVPALLSNDAASCIAVAARMIALGTHDGTVRILDLLGNQVKEFRAHT 98

Query: 65  AVVNDLSFDSEGEYVGSCSDDGSVVINSLFT-DEKMRFEYHRPMKAIALDPDYAKKTSRR 124
           A VND++FD+EGEY+GSCSDDGSVVINSLFT DEKM+F+YHRPMKAI+LDPDY KK S+R
Sbjct: 99  APVNDINFDTEGEYIGSCSDDGSVVINSLFTDDEKMKFDYHRPMKAISLDPDYTKKQSKR 158

Query: 125 FAAGGLAGHLYLNSKKWLGFKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQR 184
           F AGGLAGHLY+NSKKW G KDQVLHSGEGPIH+VKWR SLIAWAND GVKVYD A DQR
Sbjct: 159 FVAGGLAGHLYMNSKKWFGNKDQVLHSGEGPIHSVKWRGSLIAWANDVGVKVYDTAKDQR 218

Query: 185 ITFIERPRGSPRPELLLPQLVWQDDTLLVIGWGTSVKIASIRTNQNRAANGTQSSRLVPM 244
           +TFIE+PRGSPRPE LLP LVWQDDTLLVIGWGTSVKIASI+++Q +    T + R + M
Sbjct: 219 VTFIEKPRGSPRPEALLPHLVWQDDTLLVIGWGTSVKIASIKSDQQQ----TGTFRQIQM 278

Query: 245 SSMNRVDIVASFQTSYLITGMAPFGDFLVVLAYIPGE-EGEKDFSRTAP-SRQGNAQRPE 304
           SS+ +VDIVASFQTSY I+G+APFGD LV+LAYIP E +GEK+FS T   SRQGNAQRPE
Sbjct: 279 SSLTQVDIVASFQTSYYISGIAPFGDSLVILAYIPIEGDGEKEFSSTTTLSRQGNAQRPE 338

Query: 305 VRIVTWNNDELSTDALPVHGFEHYKAKDYSLAHAPFAGSSYAGGQWAAGDEPLYYVVSPK 364
           +RIV+WNNDEL+ DALPVHGFEHYKAKDYSLAHAPF GSSYAGGQWAAGDEPLYY+VSPK
Sbjct: 339 IRIVSWNNDELTMDALPVHGFEHYKAKDYSLAHAPFPGSSYAGGQWAAGDEPLYYIVSPK 398

Query: 365 DVVIAKPRDAEDHIAWLLEHGFHEKALEAVEAGQGRSELLDEVGSRYLDHLIVERKYAEA 424
           DVVIAKPRDAEDHI WLL+HGFHEKAL AVEA +GR+EL+D+VG+ YLDHLIVERKYAEA
Sbjct: 399 DVVIAKPRDAEDHINWLLQHGFHEKALAAVEASEGRTELIDKVGAGYLDHLIVERKYAEA 458

Query: 425 ASLCPKLLRGSVSAWERWVFHFAHLRQLPVLVPYIPTENPRLRDTAYEVALVALASNPLF 484
           ASLCPKLLRGS SAWERWVFHFA LRQLPVLVPY+PT+NPRL+DT YEVALVALA+NP +
Sbjct: 459 ASLCPKLLRGSASAWERWVFHFAQLRQLPVLVPYMPTDNPRLKDTVYEVALVALATNPSY 518

Query: 485 HKDLLTTVKSWPPVIYSALPVISAIEPQFNTSSMTDALKEALAELYVIDGQYEKGFLLYA 544
           HK+LL+ VKSWP  +YSAL VISAIEPQ NTSSMTDALKEALAELYVIDGQY+K F LYA
Sbjct: 519 HKELLSAVKSWPRSVYSALTVISAIEPQLNTSSMTDALKEALAELYVIDGQYQKAFSLYA 578

Query: 545 DLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPLFIQNKELIPPNEVVSQL----- 604
           DLLKP++FDFIEKY+LHEAIR KVVQLM+LDCKRA  LFIQN++LIPP+EVV QL     
Sbjct: 579 DLLKPEVFDFIEKYSLHEAIRGKVVQLMLLDCKRATVLFIQNRDLIPPSEVVPQLLKAGK 638

Query: 605 ----FKAGDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQH 664
                KAG KCD RY+++LYLH+LFEV+   GKDFHD+QVELYA+YD KMLLPFLRSSQH
Sbjct: 639 NPQVLKAGKKCDSRYYLYLYLHALFEVSHDTGKDFHDMQVELYAEYDTKMLLPFLRSSQH 698

Query: 665 YTLEKAHEICIQKNLLREQVFILGRMGNAKQALSVIIDKLGDIEEAVEFVSMQHDDELWE 724
           Y LEKA+E+C++K+ LREQVF+LGRMGNAKQAL+VII+KLGDIEEAVEFVSMQHDD+LWE
Sbjct: 699 YKLEKAYELCVKKDFLREQVFVLGRMGNAKQALAVIINKLGDIEEAVEFVSMQHDDDLWE 758

Query: 725 ELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIITDYRTETSLR 784
           ELIK CL+K EMVG+LLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKI+TDYRTETSLR
Sbjct: 759 ELIKQCLNKPEMVGLLLEHTVGNLDPLYIVNMVPNGLEIPRLRDRLVKIVTDYRTETSLR 818

Query: 785 HGCNDILKADTVNLLVKYYNEARHGIYLSNEEDEIRGKRSEN-----KVFQSIQESVSAR 844
           HGCNDILK D VNLLVK +NEAR G+ LS+E+D+ R KR +N          + +S+S +
Sbjct: 819 HGCNDILKTDIVNLLVKCFNEARRGVCLSHEDDDSRAKREDNNRSSFSQRMVVDKSLSIK 878

Query: 845 MMEVKSKTRGGARCCICFDPFSIQNMSVIVFFCCHAYHETCLIESTSNLDANKGSGETRH 904
           M EVKSKTRG  RCC+CFDP SI+  +V+VFFCCHAYHETCL+++     +N     T+ 
Sbjct: 879 MTEVKSKTRGDTRCCMCFDPVSIRGDTVVVFFCCHAYHETCLMDAAF---SNNNHKTTKG 938

Query: 905 DSTSDFDYDNG--EFEDDDEDDTDAGG-----PRMRCILCTTAASKS 928
            S  ++ YDNG  E E+D+E+D D  G      R+RCILCTTAA+ S
Sbjct: 939 SSGYEYSYDNGVDEEEEDEEEDEDGDGDRPGRSRLRCILCTTAAAAS 978