Carg23874 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23874
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Mitochondrial carrier protein
Location	Carg_Chr02: 4065433 .. 4065927 (-)
RNA-Seq Expression	Carg23874
Synteny	Carg23874

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACCGAGGCGACTGAAGCTACGACTAAGTTTCAGAGTCCGGGTTTTCGCCCGGTACCGTCGCCTCCGGATTTCCATCCCGAAATTATTGTCTCAACCCATGATGGTCTCCGGTTCTGGCAGTTCATGATTGCCGGTTCAGTCGCGGGGTCTGTGGAGCATATGGCTATGTTTCCGGTTGATACCGTCAAGACCCATATGCAAGCCCTTGGTTCTTGTCCGATTAAATCTGTTGGAGTTCGACAAGCCCTTCGGTCAATTTTGAAGTCAGAGGGACCTGCTGGTTTTTATCGTGGTATTGGTGCTATGGGCCTCGGAGCTGGTCCTGCACATGCTGTGTATTTTACAGTTTACGAGAATTGTAAAAGGGTTTTCTCTGGTGGGGATCCTAATAATTCAGTAGCCCACGCTGCTTCTGGTGTCTGTGCGACGGTGGTGAGTGATGCTGTCTTTACACCGATGGATATGGAGGAGCTGCTGCTGGAGCTTTAG

mRNA sequence

ATGGCTACCGAGGCGACTGAAGCTACGACTAAGTTTCAGAGTCCGGGTTTTCGCCCGGTACCGTCGCCTCCGGATTTCCATCCCGAAATTATTGTCTCAACCCATGATGGTCTCCGGTTCTGGCAGTTCATGATTGCCGGTTCAGTCGCGGGGTCTGTGGAGCATATGGCTATGTTTCCGGTTGATACCGTCAAGACCCATATGCAAGCCCTTGGTTCTTGTCCGATTAAATCTGTTGGAGTTCGACAAGCCCTTCGGTCAATTTTGAAGTCAGAGGGACCTGCTGGTTTTTATCGTGGTATTGGTGCTATGGGCCTCGGAGCTGGTCCTGCACATGCTGTGTATTTTACAGTTTACGAGAATTGTAAAAGGGTTTTCTCTGGTGGGGATCCTAATAATTCAGTAGCCCACGCTGCTTCTGGTGTCTGTGCGACGGTGGTGAGTGATGCTGTCTTTACACCGATGGATATGGAGGAGCTGCTGCTGGAGCTTTAG

Coding sequence (CDS)

ATGGCTACCGAGGCGACTGAAGCTACGACTAAGTTTCAGAGTCCGGGTTTTCGCCCGGTACCGTCGCCTCCGGATTTCCATCCCGAAATTATTGTCTCAACCCATGATGGTCTCCGGTTCTGGCAGTTCATGATTGCCGGTTCAGTCGCGGGGTCTGTGGAGCATATGGCTATGTTTCCGGTTGATACCGTCAAGACCCATATGCAAGCCCTTGGTTCTTGTCCGATTAAATCTGTTGGAGTTCGACAAGCCCTTCGGTCAATTTTGAAGTCAGAGGGACCTGCTGGTTTTTATCGTGGTATTGGTGCTATGGGCCTCGGAGCTGGTCCTGCACATGCTGTGTATTTTACAGTTTACGAGAATTGTAAAAGGGTTTTCTCTGGTGGGGATCCTAATAATTCAGTAGCCCACGCTGCTTCTGGTGTCTGTGCGACGGTGGTGAGTGATGCTGTCTTTACACCGATGGATATGGAGGAGCTGCTGCTGGAGCTTTAG

Protein sequence

MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYENCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL

Homology

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BLAST of Carg23874 vs. NCBI nr
Match: KAG7035342.1 (Mitoferrin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 334.7 bits (857), Expect = 4.4e-88
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60
           MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP
Sbjct: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
           NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL
Sbjct: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 164

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BLAST of Carg23874 vs. NCBI nr
Match: XP_023532261.1 (mitoferrin-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 324.7 bits (831), Expect = 4.6e-85
Identity = 159/164 (96.95%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60
           MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP
Sbjct: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
           NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDM +  L+L
Sbjct: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMVKQRLQL 164

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BLAST of Carg23874 vs. NCBI nr
Match: XP_022948031.1 (mitoferrin-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 324.7 bits (831), Expect = 4.6e-85
Identity = 159/164 (96.95%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60
           MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP
Sbjct: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
           NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDM +  L+L
Sbjct: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMVKQRLQL 164

[Close]

BLAST of Carg23874 vs. NCBI nr
Match: XP_023532262.1 (mitoferrin-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 324.7 bits (831), Expect = 4.6e-85
Identity = 159/164 (96.95%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60
           MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP
Sbjct: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
           NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDM +  L+L
Sbjct: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMVKQRLQL 164

[Close]

BLAST of Carg23874 vs. NCBI nr
Match: XP_022948032.1 (mitoferrin-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 324.7 bits (831), Expect = 4.6e-85
Identity = 159/164 (96.95%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60
           MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP
Sbjct: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
           NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDM +  L+L
Sbjct: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMVKQRLQL 164

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BLAST of Carg23874 vs. ExPASy Swiss-Prot
Match: Q23125 (Mitoferrin OS=Caenorhabditis elegans OX=6239 GN=mfn-1 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.2e-19
Identity = 53/124 (42.74%), Postives = 79/124 (63.71%), Query Frame = 0


Query: 44  MIAGSVAGSVEHMAMFPVDTVKTHMQALGSCP-IKSVGVRQALRSILKSEGPAGFYRGIG 103
           + AG++AG+VEH  MFP D+VKT MQ+L  CP  K      +L SI+K EG     RG+ 
Sbjct: 21  LTAGALAGAVEHCVMFPFDSVKTRMQSLCPCPETKCPTPVHSLMSIVKREGWLRPLRGVN 80

Query: 104 AMGLGAGPAHAVYFTVYENCKRVFSGGDP--NNSVAHAASGVCATVVSDAVFTPMDMEEL 163
           A+  G+ PAHA+YFTVYE  K   +G     +N++A+ ASGV AT++ DA+  P ++ + 
Sbjct: 81  AVAAGSMPAHALYFTVYEKMKGYLTGNSAGHSNTLAYGASGVVATLIHDAIMNPAEVVKQ 140

Query: 164 LLEL 165
            +++
Sbjct: 141 RMQM 144

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BLAST of Carg23874 vs. ExPASy Swiss-Prot
Match: Q620A6 (Mitoferrin OS=Caenorhabditis briggsae OX=6238 GN=mfn-1 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.6e-19
Identity = 52/123 (42.28%), Postives = 77/123 (62.60%), Query Frame = 0


Query: 44  MIAGSVAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGA 103
           + AG++AG+VEH  MFP D+VKT MQ+L  C  K      +L SI+K EG     RG+ A
Sbjct: 21  LAAGALAGAVEHCVMFPFDSVKTRMQSLCPCETKCPTPVHSLMSIVKREGWLRPLRGVNA 80

Query: 104 MGLGAGPAHAVYFTVYENCKRVFSGGDP--NNSVAHAASGVCATVVSDAVFTPMDMEELL 163
           +  G+ PAHA+YFTVYE  K   +G      +++A+ ASGV AT++ DAV  P ++ +  
Sbjct: 81  VAAGSMPAHALYFTVYEKMKSFLTGNTAGHEHTLAYGASGVVATLIHDAVMNPAEVVKQR 140

Query: 164 LEL 165
           +++
Sbjct: 141 MQM 143

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BLAST of Carg23874 vs. ExPASy Swiss-Prot
Match: Q55DY8 (Mitoferrin OS=Dictyostelium discoideum OX=44689 GN=mcfF PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 5.9e-19
Identity = 49/130 (37.69%), Postives = 82/130 (63.08%), Query Frame = 0


Query: 36  DGLRFWQFMIAGSVAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPA 95
           +G  F+  +IAG+ AG  EH  M+P+DT+KTH+QA+    +++  + Q  + I++  G  
Sbjct: 12  EGGSFYVHLIAGAAAGFAEHCGMYPIDTIKTHIQAIKPGAMQTSSL-QITKHIIQQHGIT 71

Query: 96  GFYRGIGAMGLGAGPAHAVYFTVYENCKRVFSGGD-PNNSVAHAASGVCATVVSDAVFTP 155
           G +RG+ A+  GA P+HAV+F++YE  K  F G D  ++ +    +G  AT+ S+AV +P
Sbjct: 72  GLFRGLTAVAAGAAPSHAVHFSIYELLKFKFIGSDEDHHPIKVGIAGAIATMTSEAVASP 131

Query: 156 MDMEELLLEL 165
           MD+ +  L+L
Sbjct: 132 MDVVKQRLQL 140

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BLAST of Carg23874 vs. ExPASy Swiss-Prot
Match: P10566 (Mitochondrial RNA-splicing protein MRS3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MRS3 PE=1 SV=4)

HSP 1 Score: 94.4 bits (233), Expect = 1.3e-18
Identity = 55/151 (36.42%), Postives = 83/151 (54.97%), Query Frame = 0


Query: 18  RPVPS-PPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFPVDTVKTHMQALGSCPI 77
           RP+P+ P D      + TH  L  +  +IAG+ AG +EH  MFP+D +KT +Q+  +  +
Sbjct: 12  RPIPAIPMDLPDYEALPTHAPL--YHQLIAGAFAGIMEHSVMFPIDALKTRIQSANAKSL 71

Query: 78  KSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYENCKRVF---SGGDPNN 137
            +  +   +  I  SEG    ++G+ ++ LGAGPAHAVYF  YE CK+     S    ++
Sbjct: 72  SAKNMLSQISHISTSEGTLALWKGVQSVILGAGPAHAVYFGTYEFCKKNLIDSSDTQTHH 131

Query: 138 SVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
               A SG CAT  SDA+  P D  +  ++L
Sbjct: 132 PFKTAISGACATTASDALMNPFDTIKQRIQL 160

[Close]

BLAST of Carg23874 vs. ExPASy Swiss-Prot
Match: Q9NYZ2 (Mitoferrin-1 OS=Homo sapiens OX=9606 GN=SLC25A37 PE=1 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 6.5e-18
Identity = 54/137 (39.42%), Postives = 81/137 (59.12%), Query Frame = 0


Query: 32  VSTHDGLRFWQFMIAGSVAGSVEHMAMFPVDTVKTHMQALGSCP-IKSVGVRQALRSILK 91
           VSTH        M AG++AG +EH  M+PVD+VKT MQ+L   P  +   +  AL+ I++
Sbjct: 45  VSTH--------MTAGAMAGILEHSVMYPVDSVKTRMQSLSPDPKAQYTSIYGALKKIMR 104

Query: 92  SEGPAGFYRGIGAMGLGAGPAHAVYFTVYENCKRVFSGGDPNNSVAHAASGVC---ATVV 151
           +EG     RG+  M +GAGPAHA+YF  YEN KR  +    +   +H A+G+    AT++
Sbjct: 105 TEGFWRPLRGVNVMIMGAGPAHAMYFACYENMKRTLNDVFHHQGNSHLANGIAGSMATLL 164

Query: 152 SDAVFTPMDMEELLLEL 165
            DAV  P ++ +  L++
Sbjct: 165 HDAVMNPAEVVKQRLQM 173

[Close]

BLAST of Carg23874 vs. ExPASy TrEMBL
Match: A0A6J1G829 (mitoferrin-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451733 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.2e-85
Identity = 159/164 (96.95%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60
           MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP
Sbjct: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
           NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDM +  L+L
Sbjct: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMVKQRLQL 164

[Close]

BLAST of Carg23874 vs. ExPASy TrEMBL
Match: A0A6J1G850 (mitoferrin-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451733 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.2e-85
Identity = 159/164 (96.95%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60
           MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP
Sbjct: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
           NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDM +  L+L
Sbjct: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMVKQRLQL 164

[Close]

BLAST of Carg23874 vs. ExPASy TrEMBL
Match: A0A6J1KXP1 (mitoferrin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499705 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.9e-84
Identity = 157/164 (95.73%), Postives = 159/164 (96.95%), Query Frame = 0


Query: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60
           MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHM MFP
Sbjct: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMTMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
           NCKRVFSGGDPNNSVAHAASGVCATV SDAVFTPMDM +  L+L
Sbjct: 121 NCKRVFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQL 164

[Close]

BLAST of Carg23874 vs. ExPASy TrEMBL
Match: A0A6J1L227 (mitoferrin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499705 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.9e-84
Identity = 157/164 (95.73%), Postives = 159/164 (96.95%), Query Frame = 0


Query: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60
           MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHM MFP
Sbjct: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMTMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
           NCKRVFSGGDPNNSVAHAASGVCATV SDAVFTPMDM +  L+L
Sbjct: 121 NCKRVFSGGDPNNSVAHAASGVCATVASDAVFTPMDMVKQRLQL 164

[Close]

BLAST of Carg23874 vs. ExPASy TrEMBL
Match: A0A5A7SZA5 (Mitoferrin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852G00170 PE=3 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.9e-81
Identity = 149/164 (90.85%), Postives = 156/164 (95.12%), Query Frame = 0


Query: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60
           MATEATEATTKFQSP FRPVPSPPDFHPEI+VS HDGLRFWQFMIAGS+AGS EHMAMFP
Sbjct: 1   MATEATEATTKFQSPDFRPVPSPPDFHPEIVVSAHDGLRFWQFMIAGSIAGSAEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           +DTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE
Sbjct: 61  IDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120

Query: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
           NCK+ FSGGDPNNS+AHAASGVCATV SDAVFTPMDM +  L+L
Sbjct: 121 NCKKFFSGGDPNNSLAHAASGVCATVASDAVFTPMDMVKQRLQL 164

[Close]

BLAST of Carg23874 vs. TAIR 10
Match: AT2G30160.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 231.9 bits (590), Expect = 3.8e-61
Identity = 112/161 (69.57%), Postives = 131/161 (81.37%), Query Frame = 0


Query: 5   ATEATTKFQSPGFRPVPSPPDFHPEIIV-STHDGLRFWQFMIAGSVAGSVEHMAMFPVDT 64
           ATEATTKF     RP+P PPDFHP IIV + +  L+FWQ M+AGS+AGSVEHMAMFPVDT
Sbjct: 2   ATEATTKFPESDLRPIPQPPDFHPAIIVPAQNTTLKFWQLMVAGSIAGSVEHMAMFPVDT 61

Query: 65  VKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYENCK 124
           VKTHMQAL SCPIK +G+RQA RSI+K++GP+  YRGI AMGLGAGPAHAVYF+ YE  K
Sbjct: 62  VKTHMQALRSCPIKPIGIRQAFRSIIKTDGPSALYRGIWAMGLGAGPAHAVYFSFYEVSK 121

Query: 125 RVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
           +  SGG+PNNS AHA SGV AT+ SDAVFTPMDM +  L++
Sbjct: 122 KFLSGGNPNNSAAHAISGVFATISSDAVFTPMDMVKQRLQI 162

[Close]

BLAST of Carg23874 vs. TAIR 10
Match: AT1G07030.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 222.6 bits (566), Expect = 2.3e-58
Identity = 112/164 (68.29%), Postives = 127/164 (77.44%), Query Frame = 0


Query: 1   MATEATEATTKFQSPGFRPVPSPPDFHPEIIVSTHDGLRFWQFMIAGSVAGSVEHMAMFP 60
           MATEAT    KFQ P  R V   PDF PEI    HDGL+FWQFMIAGS+AGSVEHMAMFP
Sbjct: 1   MATEAT-TVPKFQEPDLRQVSQTPDFKPEI---AHDGLKFWQFMIAGSIAGSVEHMAMFP 60

Query: 61  VDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAMGLGAGPAHAVYFTVYE 120
           VDT+KTHMQAL  CP+K VG+R+A RSI++ EGP+  YRGI AMGLGAGPAHAVYF+ YE
Sbjct: 61  VDTIKTHMQALRPCPLKPVGIREAFRSIIQKEGPSALYRGIWAMGLGAGPAHAVYFSFYE 120

Query: 121 NCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMDMEELLLEL 165
             K+  S GD NNSVAHA SGV AT+ SDAVFTPMDM +  L++
Sbjct: 121 VSKKYLSAGDQNNSVAHAMSGVFATISSDAVFTPMDMVKQRLQM 160

[Close]

BLAST of Carg23874 vs. TAIR 10
Match: AT1G07025.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 108.6 bits (270), Expect = 4.8e-24
Identity = 62/124 (50.00%), Postives = 79/124 (63.71%), Query Frame = 0


Query: 38  LRFWQFMIAGSVAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGF 97
           L+ WQ MIAGSVAGS ++M MFPV T+   M    S   + VG+RQALRS++++EGP+  
Sbjct: 16  LQLWQLMIAGSVAGSFKNMTMFPVRTLDQRM-LHRSYSQRHVGIRQALRSVIQTEGPSAL 75

Query: 98  YRGIGAMGLGA-GPAHAVYFTVYENCKRVFSGGDPNNSVAHAASGVCATVVSDAVFTPMD 157
           YRGI  M  GA GPA  V+F+ Y+  K   S G+PNN V H  S     V S AV TP+D
Sbjct: 76  YRGIWYMRHGAMGPAQFVHFSFYDVSKNFLSTGNPNNPVVHVISWAFTAVWSYAVSTPVD 135

Query: 158 MEEL 161
           M +L
Sbjct: 136 MAKL 138

[Close]

BLAST of Carg23874 vs. TAIR 10
Match: AT2G37890.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 68.6 bits (166), Expect = 5.5e-12
Identity = 40/115 (34.78%), Postives = 60/115 (52.17%), Query Frame = 0


Query: 45  IAGSVAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAM 104
           ++G +AG     A +P+D V+T + A  +  I   G+    R+I + EG  G Y+G+GA 
Sbjct: 153 VSGGLAGITAATATYPLDLVRTRLAAQRNA-IYYQGIEHTFRTICREEGILGLYKGLGAT 212

Query: 105 GLGAGPAHAVYFTVYENCKRVFSGGDPNNS--VAHAASGVCATVVSDAVFTPMDM 158
            LG GP+ A+ F  YE+ K  +    PN+S  V    SG  A  VS     P+D+
Sbjct: 213 LLGVGPSLAINFAAYESMKLFWHSHRPNDSDLVVSLVSGGLAGAVSSTATYPLDL 266

[Close]

BLAST of Carg23874 vs. TAIR 10
Match: AT3G53940.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 68.2 bits (165), Expect = 7.2e-12
Identity = 41/122 (33.61%), Postives = 63/122 (51.64%), Query Frame = 0


Query: 45  IAGSVAGSVEHMAMFPVDTVKTHMQALGSCPIKSVGVRQALRSILKSEGPAGFYRGIGAM 104
           ++G +AG     A +P+D V+T + A  +  I   GV  A R+I + EG  G Y+G+GA 
Sbjct: 181 VSGGLAGLTAASATYPLDLVRTRLSAQRN-SIYYQGVGHAFRTICREEGILGLYKGLGAT 240

Query: 105 GLGAGPAHAVYFTVYENCKRVFSGGDPN--NSVAHAASGVCATVVSDAVFTPMDMEELLL 164
            LG GP+ A+ F  YE  K  +    PN  N+V     G  + +VS     P+D+    +
Sbjct: 241 LLGVGPSLAISFAAYETFKTFWLSHRPNDSNAVVSLGCGSLSGIVSSTATFPLDLVRRRM 300

The following BLAST results are available for this feature:

BLAST of Carg23874 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035342.1	4.4e-88	100.00	Mitoferrin, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023532261.1	4.6e-85	96.95	mitoferrin-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022948031.1	4.6e-85	96.95	mitoferrin-like isoform X1 [Cucurbita moschata]	[more]
XP_023532262.1	4.6e-85	96.95	mitoferrin-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022948032.1	4.6e-85	96.95	mitoferrin-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Carg23874 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q23125	1.2e-19	42.74	Mitoferrin OS=Caenorhabditis elegans OX=6239 GN=mfn-1 PE=2 SV=1	[more]
Q620A6	1.6e-19	42.28	Mitoferrin OS=Caenorhabditis briggsae OX=6238 GN=mfn-1 PE=3 SV=1	[more]
Q55DY8	5.9e-19	37.69	Mitoferrin OS=Dictyostelium discoideum OX=44689 GN=mcfF PE=3 SV=1	[more]
P10566	1.3e-18	36.42	Mitochondrial RNA-splicing protein MRS3 OS=Saccharomyces cerevisiae (strain ATCC...	[more]
Q9NYZ2	6.5e-18	39.42	Mitoferrin-1 OS=Homo sapiens OX=9606 GN=SLC25A37 PE=1 SV=2	[more]

BLAST of Carg23874 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G829	2.2e-85	96.95	mitoferrin-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451733 PE=3 SV...	[more]
A0A6J1G850	2.2e-85	96.95	mitoferrin-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451733 PE=3 SV...	[more]
A0A6J1KXP1	1.9e-84	95.73	mitoferrin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499705 PE=3 SV=1	[more]
A0A6J1L227	1.9e-84	95.73	mitoferrin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499705 PE=3 SV=1	[more]
A0A5A7SZA5	1.9e-81	90.85	Mitoferrin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852G0017...	[more]

BLAST of Carg23874 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30160.1	3.8e-61	69.57	Mitochondrial substrate carrier family protein	[more]
AT1G07030.1	2.3e-58	68.29	Mitochondrial substrate carrier family protein	[more]
AT1G07025.1	4.8e-24	50.00	Mitochondrial substrate carrier family protein	[more]
AT2G37890.1	5.5e-12	34.78	Mitochondrial substrate carrier family protein	[more]
AT3G53940.1	7.2e-12	33.61	Mitochondrial substrate carrier family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023395	Mitochondrial carrier domain superfamily	GENE3D	1.50.40.10	Mitochondrial carrier domain	coord: 11..158 e-value: 5.0E-32 score: 112.8
IPR023395	Mitochondrial carrier domain superfamily	SUPERFAMILY	103506	Mitochondrial carrier	coord: 36..157
IPR018108	Mitochondrial substrate/solute carrier	PFAM	PF00153	Mito_carr	coord: 38..127 e-value: 3.1E-22 score: 78.3
IPR018108	Mitochondrial substrate/solute carrier	PROSITE	PS50920	SOLCAR	coord: 38..125 score: 21.140013
None	No IPR available	PANTHER	PTHR45758	MITOFERRIN-1-RELATED	coord: 5..158
None	No IPR available	PANTHER	PTHR45758:SF11	MITOCHONDRIAL CARRIER PROTEIN, EXPRESSED	coord: 5..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23874-RA	Carg23874-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048250	iron import into the mitochondrion
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005381	iron ion transmembrane transporter activity