Carg23849 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23849
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionpleckstrin homology domain-containing protein 1-like
LocationCarg_Chr02: 4221747 .. 4222181 (-)
RNA-Seq ExpressionCarg23849
SyntenyCarg23849
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGCCTGTGGCGATCGGCAACCGGCCAAGATCCAAGTCCAGAGGATTACAAGGGCGTCGAATTCTGGAACAGTCCCGAACGCGCCGGCTGGCTCAACAAGCAGGGTGAATACTTCAAAACCTGGCGTCGCCGTTGGTTCGTTCTGAAACAAGGTAAACTCTTGTGGTTTAAGGACTCTGTTGTTACTCGCGCTTCCATTCCACGCGGTGTCATCCCTGTCAATACCTGCCTCACTGTCAAGGGCGCTGAGGATGTCCTCCATAAGCCTTGCGCCTTCGAGCTCTCTACCACTGGTCAAGACACCATGTATTTTATCGCCAAGTCGGAGCGCGAAAAGGAGGAGTGGATTAATTCGATCGGCCGCTCGATAGTTCAGAATTCGCGATCGGTTACCGAATCTGAAGTCGTTGATTACGATAACAGGCGATGA

mRNA sequence

ATGGAGAGCCTGTGGCGATCGGCAACCGGCCAAGATCCAAGTCCAGAGGATTACAAGGGCGTCGAATTCTGGAACAGTCCCGAACGCGCCGGCTGGCTCAACAAGCAGGGTGAATACTTCAAAACCTGGCGTCGCCGTTGGTTCGTTCTGAAACAAGGTAAACTCTTGTGGTTTAAGGACTCTGTTGTTACTCGCGCTTCCATTCCACGCGGTGTCATCCCTGTCAATACCTGCCTCACTGTCAAGGGCGCTGAGGATGTCCTCCATAAGCCTTGCGCCTTCGAGCTCTCTACCACTGGTCAAGACACCATGTATTTTATCGCCAAGTCGGAGCGCGAAAAGGAGGAGTGGATTAATTCGATCGGCCGCTCGATAGTTCAGAATTCGCGATCGGTTACCGAATCTGAAGTCGTTGATTACGATAACAGGCGATGA

Coding sequence (CDS)

ATGGAGAGCCTGTGGCGATCGGCAACCGGCCAAGATCCAAGTCCAGAGGATTACAAGGGCGTCGAATTCTGGAACAGTCCCGAACGCGCCGGCTGGCTCAACAAGCAGGGTGAATACTTCAAAACCTGGCGTCGCCGTTGGTTCGTTCTGAAACAAGGTAAACTCTTGTGGTTTAAGGACTCTGTTGTTACTCGCGCTTCCATTCCACGCGGTGTCATCCCTGTCAATACCTGCCTCACTGTCAAGGGCGCTGAGGATGTCCTCCATAAGCCTTGCGCCTTCGAGCTCTCTACCACTGGTCAAGACACCATGTATTTTATCGCCAAGTCGGAGCGCGAAAAGGAGGAGTGGATTAATTCGATCGGCCGCTCGATAGTTCAGAATTCGCGATCGGTTACCGAATCTGAAGTCGTTGATTACGATAACAGGCGATGA

Protein sequence

MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKDSVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINSIGRSIVQNSRSVTESEVVDYDNRR
Homology
BLAST of Carg23849 vs. NCBI nr
Match: XP_022947886.1 (pleckstrin homology domain-containing protein 1-like [Cucurbita moschata] >XP_023532322.1 pleckstrin homology domain-containing protein 1-like [Cucurbita pepo subsp. pepo] >KAG6605417.1 Pleckstrin-likey domain-containing protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035366.1 Pleckstrin-likey domain-containing protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 302.4 bits (773), Expect = 2.1e-78
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD
Sbjct: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60

Query: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120
           SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS
Sbjct: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120

Query: 121 IGRSIVQNSRSVTESEVVDYDNRR 145
           IGRSIVQNSRSVTESEVVDYDNRR
Sbjct: 121 IGRSIVQNSRSVTESEVVDYDNRR 144

BLAST of Carg23849 vs. NCBI nr
Match: XP_023007310.1 (pleckstrin homology domain-containing protein 1-like [Cucurbita maxima])

HSP 1 Score: 301.2 bits (770), Expect = 4.7e-78
Identity = 143/144 (99.31%), Postives = 144/144 (100.00%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MESLWR+ATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD
Sbjct: 1   MESLWRAATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60

Query: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120
           SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS
Sbjct: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120

Query: 121 IGRSIVQNSRSVTESEVVDYDNRR 145
           IGRSIVQNSRSVTESEVVDYDNRR
Sbjct: 121 IGRSIVQNSRSVTESEVVDYDNRR 144

BLAST of Carg23849 vs. NCBI nr
Match: KAE8646952.1 (hypothetical protein Csa_023953, partial [Cucumis sativus])

HSP 1 Score: 290.0 bits (741), Expect = 1.1e-74
Identity = 135/144 (93.75%), Postives = 141/144 (97.92%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MESLWR+ATGQDPSPEDYKGVEFW SPERAGWLNKQGEY +TWRRRWFVLK+GKL WFKD
Sbjct: 39  MESLWRAATGQDPSPEDYKGVEFWTSPERAGWLNKQGEYLRTWRRRWFVLKRGKLFWFKD 98

Query: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120
           S+VTRASIPRGVIPVNTCLTVKGAED+LHKPCAFELSTTGQDTMYFIA+SEREKEEWINS
Sbjct: 99  SIVTRASIPRGVIPVNTCLTVKGAEDILHKPCAFELSTTGQDTMYFIAESEREKEEWINS 158

Query: 121 IGRSIVQNSRSVTESEVVDYDNRR 145
           IGRSIVQNSRSVTESEVVDYDNRR
Sbjct: 159 IGRSIVQNSRSVTESEVVDYDNRR 182

BLAST of Carg23849 vs. NCBI nr
Match: XP_004143153.1 (pleckstrin homology domain-containing protein 1 [Cucumis sativus] >XP_008464048.1 PREDICTED: pleckstrin homology domain-containing protein 1 [Cucumis melo] >KAA0061839.1 pleckstrin-like proteiny domain-containing protein 1 [Cucumis melo var. makuwa] >TYK15417.1 pleckstrin-like proteiny domain-containing protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 290.0 bits (741), Expect = 1.1e-74
Identity = 135/144 (93.75%), Postives = 141/144 (97.92%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MESLWR+ATGQDPSPEDYKGVEFW SPERAGWLNKQGEY +TWRRRWFVLK+GKL WFKD
Sbjct: 6   MESLWRAATGQDPSPEDYKGVEFWTSPERAGWLNKQGEYLRTWRRRWFVLKRGKLFWFKD 65

Query: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120
           S+VTRASIPRGVIPVNTCLTVKGAED+LHKPCAFELSTTGQDTMYFIA+SEREKEEWINS
Sbjct: 66  SIVTRASIPRGVIPVNTCLTVKGAEDILHKPCAFELSTTGQDTMYFIAESEREKEEWINS 125

Query: 121 IGRSIVQNSRSVTESEVVDYDNRR 145
           IGRSIVQNSRSVTESEVVDYDNRR
Sbjct: 126 IGRSIVQNSRSVTESEVVDYDNRR 149

BLAST of Carg23849 vs. NCBI nr
Match: XP_022944633.1 (pleckstrin homology domain-containing protein 1-like [Cucurbita moschata] >XP_022986962.1 pleckstrin homology domain-containing protein 1-like [Cucurbita maxima] >XP_023512687.1 pleckstrin homology domain-containing protein 1-like [Cucurbita pepo subsp. pepo] >KAG6570660.1 Pleckstrin-likey domain-containing protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010508.1 Pleckstrin-likey domain-containing protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 289.7 bits (740), Expect = 1.4e-74
Identity = 135/144 (93.75%), Postives = 140/144 (97.22%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MESLWR+ATGQDPSP+DYKGVEFW SPERAGWLNKQGEY KTWRRRWFVLKQGKL WFKD
Sbjct: 1   MESLWRAATGQDPSPDDYKGVEFWTSPERAGWLNKQGEYLKTWRRRWFVLKQGKLFWFKD 60

Query: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120
           S+VTRASIPRGVIPVNTCLTVKGAED+LHKPCAFELSTTG DTMYFIA+SEREKEEWINS
Sbjct: 61  SIVTRASIPRGVIPVNTCLTVKGAEDILHKPCAFELSTTGHDTMYFIAESEREKEEWINS 120

Query: 121 IGRSIVQNSRSVTESEVVDYDNRR 145
           IGRSIVQNSRSVTESEVVDYDNRR
Sbjct: 121 IGRSIVQNSRSVTESEVVDYDNRR 144

BLAST of Carg23849 vs. ExPASy Swiss-Prot
Match: Q9ST43 (Pleckstrin homology domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 GN=PH1 PE=2 SV=2)

HSP 1 Score: 225.7 bits (574), Expect = 3.3e-58
Identity = 108/146 (73.97%), Postives = 126/146 (86.30%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MES+WR ATGQDPS EDY+G+EFW++PER+GWL KQG+Y KTWRRRWFVLK+GKLLWFKD
Sbjct: 1   MESIWRIATGQDPSREDYEGIEFWSNPERSGWLTKQGDYIKTWRRRWFVLKRGKLLWFKD 60

Query: 61  SVVT--RASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWI 120
                 R S PRGVI V  CLTVKGAEDV++KP AFELS +G  TM+FIA +E+EKEEWI
Sbjct: 61  QAAAGIRGSTPRGVISVGDCLTVKGAEDVVNKPFAFELS-SGSYTMFFIADNEKEKEEWI 120

Query: 121 NSIGRSIVQNSRSVTESEVVDYDNRR 145
           NSIGRSIVQ+SRSVT+SEV+DYD+RR
Sbjct: 121 NSIGRSIVQHSRSVTDSEVLDYDHRR 145

BLAST of Carg23849 vs. ExPASy Swiss-Prot
Match: Q86IV4 (PH domain-containing protein DDB_G0274775 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274775 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.3e-09
Identity = 36/98 (36.73%), Postives = 52/98 (53.06%), Query Frame = 0

Query: 28  ERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKDSVVTRASIPRGVIPVNTCLTVKGAEDV 87
           +R GWL KQG   +TWRRRWFVLK  KL +FK      A+   G+I +     VK  +D 
Sbjct: 17  DREGWLTKQGGSIRTWRRRWFVLKGKKLFYFKSKGDIEAT---GLIELEQNSFVKEEKDK 76

Query: 88  LHKPCAFELSTTGQDTMYFIAKSEREKEEWINSIGRSI 126
             K        T +   Y  A++E + ++W+ SI R++
Sbjct: 77  DKKKKYMFTVGTSKRVFYIFAETETDMKQWMESIKRNL 111

BLAST of Carg23849 vs. ExPASy Swiss-Prot
Match: O08967 (Cytohesin-3 OS=Mus musculus OX=10090 GN=Cyth3 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.7e-09
Identity = 45/121 (37.19%), Postives = 58/121 (47.93%), Query Frame = 0

Query: 26  SPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKDSVVTRASIPRGVIPVNTCLTVKGAE 85
           +P+R GWL K G   KTW+RRWF+L    L +F+    T    PRG+IP+   L+++  E
Sbjct: 264 NPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFE---YTTDKEPRGIIPLEN-LSIREVE 323

Query: 86  DVLHKPCAFEL--------------------STTGQDTMYFI-AKSEREKEEWINSIGRS 126
           D   KP  FEL                       G   +Y I A S  EKEEW+ SI  S
Sbjct: 324 DP-RKPNCFELYNPSHKGQVIKACKTEADGRVVEGNHVVYRISAPSPEEKEEWMKSIKAS 379

BLAST of Carg23849 vs. ExPASy Swiss-Prot
Match: Q76MY7 (Cytohesin-2 OS=Chlorocebus aethiops OX=9534 GN=CYTH2 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.4e-08
Identity = 41/121 (33.88%), Postives = 59/121 (48.76%), Query Frame = 0

Query: 26  SPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKDSVVTRASIPRGVIPVNTCLTVKGAE 85
           +P+R GWL K G   KTW+RRWF+L    L +F+    T    PRG+IP+   L+++  +
Sbjct: 259 NPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFE---YTTDKEPRGIIPLEN-LSIREVD 318

Query: 86  DVLHKPCAFEL--------------------STTGQDTMYFI-AKSEREKEEWINSIGRS 126
           D   KP  FEL                       G   +Y I A ++ EK+EWI SI  +
Sbjct: 319 DP-RKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPTQEEKDEWIKSIQAA 374

BLAST of Carg23849 vs. ExPASy Swiss-Prot
Match: Q80TI1 (Pleckstrin homology domain-containing family H member 1 OS=Mus musculus OX=10090 GN=Plekhh1 PE=1 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 3.2e-08
Identity = 36/92 (39.13%), Postives = 54/92 (58.70%), Query Frame = 0

Query: 28  ERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD-SVVTRASIPRGVIPVNTCLTVKGAED 87
           E++G+L K G   KTW+RRWFVL+QG++L++K  S V R   P+G + +N+   +   E+
Sbjct: 574 EKSGYLLKMGSRVKTWKRRWFVLRQGQILYYKSPSDVIRK--PQGQVDLNSHCQIVREEE 633

Query: 88  VLHKPCAFELSTTGQDTMYFIAKSEREKEEWI 119
                  F+L  +G  T Y  A+S    EEWI
Sbjct: 634 AQ----TFQL-ISGNKTYYLTAESPSLLEEWI 658

BLAST of Carg23849 vs. ExPASy TrEMBL
Match: A0A6J1G7Q8 (pleckstrin homology domain-containing protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451639 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.0e-78
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD
Sbjct: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60

Query: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120
           SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS
Sbjct: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120

Query: 121 IGRSIVQNSRSVTESEVVDYDNRR 145
           IGRSIVQNSRSVTESEVVDYDNRR
Sbjct: 121 IGRSIVQNSRSVTESEVVDYDNRR 144

BLAST of Carg23849 vs. ExPASy TrEMBL
Match: A0A6J1L2M0 (pleckstrin homology domain-containing protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499846 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.3e-78
Identity = 143/144 (99.31%), Postives = 144/144 (100.00%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MESLWR+ATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD
Sbjct: 1   MESLWRAATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60

Query: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120
           SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS
Sbjct: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120

Query: 121 IGRSIVQNSRSVTESEVVDYDNRR 145
           IGRSIVQNSRSVTESEVVDYDNRR
Sbjct: 121 IGRSIVQNSRSVTESEVVDYDNRR 144

BLAST of Carg23849 vs. ExPASy TrEMBL
Match: A0A5A7V834 (Pleckstrin-like proteiny domain-containing protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00610 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.3e-75
Identity = 135/144 (93.75%), Postives = 141/144 (97.92%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MESLWR+ATGQDPSPEDYKGVEFW SPERAGWLNKQGEY +TWRRRWFVLK+GKL WFKD
Sbjct: 6   MESLWRAATGQDPSPEDYKGVEFWTSPERAGWLNKQGEYLRTWRRRWFVLKRGKLFWFKD 65

Query: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120
           S+VTRASIPRGVIPVNTCLTVKGAED+LHKPCAFELSTTGQDTMYFIA+SEREKEEWINS
Sbjct: 66  SIVTRASIPRGVIPVNTCLTVKGAEDILHKPCAFELSTTGQDTMYFIAESEREKEEWINS 125

Query: 121 IGRSIVQNSRSVTESEVVDYDNRR 145
           IGRSIVQNSRSVTESEVVDYDNRR
Sbjct: 126 IGRSIVQNSRSVTESEVVDYDNRR 149

BLAST of Carg23849 vs. ExPASy TrEMBL
Match: A0A0A0KBN3 (PH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G186365 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.3e-75
Identity = 135/144 (93.75%), Postives = 141/144 (97.92%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MESLWR+ATGQDPSPEDYKGVEFW SPERAGWLNKQGEY +TWRRRWFVLK+GKL WFKD
Sbjct: 6   MESLWRAATGQDPSPEDYKGVEFWTSPERAGWLNKQGEYLRTWRRRWFVLKRGKLFWFKD 65

Query: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120
           S+VTRASIPRGVIPVNTCLTVKGAED+LHKPCAFELSTTGQDTMYFIA+SEREKEEWINS
Sbjct: 66  SIVTRASIPRGVIPVNTCLTVKGAEDILHKPCAFELSTTGQDTMYFIAESEREKEEWINS 125

Query: 121 IGRSIVQNSRSVTESEVVDYDNRR 145
           IGRSIVQNSRSVTESEVVDYDNRR
Sbjct: 126 IGRSIVQNSRSVTESEVVDYDNRR 149

BLAST of Carg23849 vs. ExPASy TrEMBL
Match: A0A1S3CL23 (pleckstrin homology domain-containing protein 1 OS=Cucumis melo OX=3656 GN=LOC103502029 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.3e-75
Identity = 135/144 (93.75%), Postives = 141/144 (97.92%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MESLWR+ATGQDPSPEDYKGVEFW SPERAGWLNKQGEY +TWRRRWFVLK+GKL WFKD
Sbjct: 6   MESLWRAATGQDPSPEDYKGVEFWTSPERAGWLNKQGEYLRTWRRRWFVLKRGKLFWFKD 65

Query: 61  SVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWINS 120
           S+VTRASIPRGVIPVNTCLTVKGAED+LHKPCAFELSTTGQDTMYFIA+SEREKEEWINS
Sbjct: 66  SIVTRASIPRGVIPVNTCLTVKGAEDILHKPCAFELSTTGQDTMYFIAESEREKEEWINS 125

Query: 121 IGRSIVQNSRSVTESEVVDYDNRR 145
           IGRSIVQNSRSVTESEVVDYDNRR
Sbjct: 126 IGRSIVQNSRSVTESEVVDYDNRR 149

BLAST of Carg23849 vs. TAIR 10
Match: AT2G29700.1 (pleckstrin homologue 1 )

HSP 1 Score: 225.7 bits (574), Expect = 2.4e-59
Identity = 108/146 (73.97%), Postives = 126/146 (86.30%), Query Frame = 0

Query: 1   MESLWRSATGQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFKD 60
           MES+WR ATGQDPS EDY+G+EFW++PER+GWL KQG+Y KTWRRRWFVLK+GKLLWFKD
Sbjct: 1   MESIWRIATGQDPSREDYEGIEFWSNPERSGWLTKQGDYIKTWRRRWFVLKRGKLLWFKD 60

Query: 61  SVVT--RASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWI 120
                 R S PRGVI V  CLTVKGAEDV++KP AFELS +G  TM+FIA +E+EKEEWI
Sbjct: 61  QAAAGIRGSTPRGVISVGDCLTVKGAEDVVNKPFAFELS-SGSYTMFFIADNEKEKEEWI 120

Query: 121 NSIGRSIVQNSRSVTESEVVDYDNRR 145
           NSIGRSIVQ+SRSVT+SEV+DYD+RR
Sbjct: 121 NSIGRSIVQHSRSVTDSEVLDYDHRR 145

BLAST of Carg23849 vs. TAIR 10
Match: AT5G05710.1 (Pleckstrin homology (PH) domain superfamily protein )

HSP 1 Score: 222.2 bits (565), Expect = 2.6e-58
Identity = 108/144 (75.00%), Postives = 122/144 (84.72%), Query Frame = 0

Query: 1   MESLWRSAT-GQDPSPEDYKGVEFWNSPERAGWLNKQGEYFKTWRRRWFVLKQGKLLWFK 60
           M SLWR+   GQ+ + EDY GVEFW++PER GWL KQGEY KTWRRRWFVLKQGKL WFK
Sbjct: 1   MASLWRAVIGGQNNNSEDYGGVEFWSNPERTGWLTKQGEYIKTWRRRWFVLKQGKLFWFK 60

Query: 61  DSVVTRASIPRGVIPVNTCLTVKGAEDVLHKPCAFELSTTGQDTMYFIAKSEREKEEWIN 120
           DS VTR S PRGV+PV +CLT KGAEDVL+K  AFELST   +TMYFIA SE+EKE+WIN
Sbjct: 61  DSDVTRVSRPRGVVPVESCLTAKGAEDVLNKQNAFELSTR-NETMYFIADSEKEKEDWIN 120

Query: 121 SIGRSIVQNSRSVTESEVVDYDNR 144
           SIGRSIVQNSRSVT+SE+VDYDN+
Sbjct: 121 SIGRSIVQNSRSVTDSEIVDYDNK 143

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022947886.12.1e-78100.00pleckstrin homology domain-containing protein 1-like [Cucurbita moschata] >XP_02... [more]
XP_023007310.14.7e-7899.31pleckstrin homology domain-containing protein 1-like [Cucurbita maxima][more]
KAE8646952.11.1e-7493.75hypothetical protein Csa_023953, partial [Cucumis sativus][more]
XP_004143153.11.1e-7493.75pleckstrin homology domain-containing protein 1 [Cucumis sativus] >XP_008464048.... [more]
XP_022944633.11.4e-7493.75pleckstrin homology domain-containing protein 1-like [Cucurbita moschata] >XP_02... [more]
Match NameE-valueIdentityDescription
Q9ST433.3e-5873.97Pleckstrin homology domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q86IV41.3e-0936.73PH domain-containing protein DDB_G0274775 OS=Dictyostelium discoideum OX=44689 G... [more]
O089671.7e-0937.19Cytohesin-3 OS=Mus musculus OX=10090 GN=Cyth3 PE=1 SV=1[more]
Q76MY71.4e-0833.88Cytohesin-2 OS=Chlorocebus aethiops OX=9534 GN=CYTH2 PE=2 SV=1[more]
Q80TI13.2e-0839.13Pleckstrin homology domain-containing family H member 1 OS=Mus musculus OX=10090... [more]
Match NameE-valueIdentityDescription
A0A6J1G7Q81.0e-78100.00pleckstrin homology domain-containing protein 1-like OS=Cucurbita moschata OX=36... [more]
A0A6J1L2M02.3e-7899.31pleckstrin homology domain-containing protein 1-like OS=Cucurbita maxima OX=3661... [more]
A0A5A7V8345.3e-7593.75Pleckstrin-like proteiny domain-containing protein 1 OS=Cucumis melo var. makuwa... [more]
A0A0A0KBN35.3e-7593.75PH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G186365 PE=4 SV=... [more]
A0A1S3CL235.3e-7593.75pleckstrin homology domain-containing protein 1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT2G29700.12.4e-5973.97pleckstrin homologue 1 [more]
AT5G05710.12.6e-5875.00Pleckstrin homology (PH) domain superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001849Pleckstrin homology domainSMARTSM00233PH_updatecoord: 27..128
e-value: 1.7E-21
score: 87.4
IPR001849Pleckstrin homology domainPFAMPF00169PHcoord: 28..124
e-value: 5.2E-21
score: 75.1
IPR001849Pleckstrin homology domainPROSITEPS50003PH_DOMAINcoord: 26..126
score: 17.251301
IPR011993PH-like domain superfamilyGENE3D2.30.29.30coord: 24..137
e-value: 4.4E-27
score: 96.2
NoneNo IPR availablePANTHERPTHR22902SESQUIPEDALIANcoord: 1..144
NoneNo IPR availablePANTHERPTHR22902:SF26PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING PROTEIN 1coord: 1..144
NoneNo IPR availableCDDcd13276PH_AtPH1coord: 29..135
e-value: 7.73783E-57
score: 170.961
NoneNo IPR availableSUPERFAMILY50729PH domain-likecoord: 24..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23849-RACarg23849-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0032266 phosphatidylinositol-3-phosphate binding