Carg23839 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23839
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein NRT1/ PTR FAMILY 8.1-like
LocationCarg_Chr02: 4265190 .. 4267899 (+)
RNA-Seq ExpressionCarg23839
SyntenyCarg23839
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAATTATTTCTAAAGTTTTAAGAATAAAATAGCCAATTTCCAAATCCTAGGAATAAATAGTAATTTAATTTAGTCAAAATGAAAATTATGTTGAAGCACTGGTCTAAGATGTCCTAATGGCTAGCGATGAACTCCTTGAATAGTCCGTCAGTCCCTCGGTTTCCAGTTTTCGACGGCGCTATTTAAACCATCGCCGCCAATTCAAGATATAAACAAACGCCTAATTGCCGAAACCGAGTTCTTGTTCTAAAGCTCAGTAATGGAGTCCCCAGTGATGCGGTTCATAGGTCCCACCACTTCTCCTTGTTTCTCATTCCAAGTTTGAACGCCTCGACTCTCAAGACTAAGGTCACTACTCATTATCTTCCACTTATTGCTTTTCAAAATTCCATACAAATCTCTGCAAATTGCTTTTCCTGTCCCGTCGGTTCGTTCTGGATGCGATTCTCCATTTAGGTATGTTCATTTTCCCCCAAATCTGTTAGGTAAGGAAATGAATCTTCGAAACGACAAGCTATTTTCTCGTTTCTGAAATTTCGTTTGATCTCTGTCTGATTCTTGGAGATCCTGAAACCTAACCATGGAGGAAGAGGATAGTATTTACACGCAAGATGGAACGGTGGACTACCGTGGAGATCCTGCGATTAGAAGCCAAACAGGAACCTGGAAGGCCTGTCCCTATATTTTGGGTAGTTTTTCTGTTCTGGAAAAGTTAGGTCGATGAGTTTTCATATGGAATTGAACTGATGATTTCTATGACTTCTGAAATTTACTTGTTGATTCTTGAATGAATAGGCAATGAATTTTGTGAGAGATTGGCTTACTATGGAATGAGCACGAACTTGGTGCTCTACTTTAAGAAACATTTGAATCAGCACAGTGCTACCGCTGTTAAGAATGTGGCTAATTGGAGTGGAACCTGCTACATTACTCCCTTGATTGGAGCTTTTCTGGCTGATGCCTACTTGGGAAGATATCGAACTATTGCTACCTTCTCCATCATCTATGTTATCGTGAGTTCCTCTCTCCAAAACTTGTGGATTGCTACCTTCTCTATCATCTATGTTCTTGTGAGTTTTCTCTTGGAAACTTGTAGAAGATAGAGATCTATTGATACGATCAAATTTGATCGTGTAGCTCAACTAAGTTAAAACATATACTCTAAACTAAGAAGTGAATTCGAATCTAACTCCGGAATTGGTGTTTGAGAGTTTAAGCTTCAATGGGAAATTGTGATATATTTGCAGGGGATGACACTTTTGACATTGTCAGCTTCAGTTCATGGTTTGAAGCCCACATGTGTTGCTAAAGATGATTGTCAAGCGACGACTGCACAAAGTGCGGTGTGTTTTGTGGCTTTGTACTTGATTGCATTGGGAACTGGTGGGATTAAGCCTTGTGTTTCGTCTTACGGAGCTGATCAATTTGATGATGCCAATGAAACTGAGAAGAAGCACAAGAGCTCTTTCTTTAATTGGTTCTATCTTTCAATCAATGTTGGTGCTCTTATAGCAAGTTCTGTGCTTGTTTGGGTGCAAGATAATGTGAGTTGGGCTTGGGGGTTTGGTATTCCAGCCATAGCTATGGCGATTGCGGTGGCAAGTTTCTTTTCGGGGACTCGGTTGTTTCGGAACCAGAAACCTGGAGGAAGTCCTTTTACACGGATATCTCAGGTCATGGTGGCATCGTTTAGAAAATATAAGGTGAAAGTACCTGAAAGCAAGGCTTTGTACGAGACTGCTGATTCTGAGTCTTCTATCGTAGGAAGCCGCAAACTCGACCACACCGATGATTTCAGGTTCGATTTTAAAACTGAAAACCCTGCCTTGAAGCACTTCTTAAGAGCTACTCATACCCATTTTGTTTGAATTCAGGTTCTTTGACAAGGCAGCAGTGGAGATCGAATCAGATCAGATGTTAAAGGGCTCAGTAAATCCGTGGAGGCTTTGCACTGTCACTCAAGTGGAGGAGCTAAAGGCCATCATAAGGTTGCTTCCAGTTTGGGCAACTGGGATTGTATTTGCTGCTGTATATAGTCAGATGGGCAATTTATTTGTGTTGCAAGGTGATAAAATGGATGCTCATATTGGCCCCAACTTTGAGATCCCAGCAGCATCTCTATCCATTTTCGACACTTTGAGCGTGATCTTTTGGGTCCCGATTTATGATCGAATCATAGTTCCAGTGGCTCGAAAATACACAGGTCATCCAAACGGTATAACTCAACTGCAGAGAATGGGCATTGGCCTATTTATTTCAATTCTAGCTATGCTATCTGCAGCAATCTTAGAGCTTGTGAGACTTCGAGAAGTGAGAAAGAACGACTATTATCTACTCGAAAAGATGCCAATGTCCATCTTCTGGCAAGTTCCTCAGTATTTTCTTATTGGTTGTGCTGAAGTATTTACCTTAATAGGCCAATTGGAGTTCTTTTACGAGCAAGCTCCCGACGCCATGAGAAGTCTCTGCTCCGCTCTCTCGCTTACTACCATTGCACTTGGAAACTACCTAAGCTCTCTACTCGTGACTATCGTCACCAAAGTCAGCACTAAAGATGGGAGTCTTGGATGGATACCAGACAACTTGAACTACGGTCATATACAATATTTCTTCTTTCTCTTGGTGATCTTGAGTATCAAAAATTTGATTGCTTTCCTTTTCATAGCCAAGTGGTACAAGTATAAGAGACCCATTGGAACAGTTCGCTGA

mRNA sequence

AGAATTATTTCTAAAGTTTTAAGAATAAAATAGCCAATTTCCAAATCCTAGGAATAAATAGTAATTTAATTTAGTCAAAATGAAAATTATGTTGAAGCACTGGTCTAAGATGTCCTAATGGCTAGCGATGAACTCCTTGAATAGTCCGTCAGTCCCTCGGTTTCCAGTTTTCGACGGCGCTATTTAAACCATCGCCGCCAATTCAAGATATAAACAAACGCCTAATTGCCGAAACCGAGTTCTTGTTCTAAAGCTCAGTAATGGAGTCCCCAGTGATGCGGTTCATAGGTCCCACCACTTCTCCTTGTTTCTCATTCCAAGTTTGAACGCCTCGACTCTCAAGACTAAGGTCACTACTCATTATCTTCCACTTATTGCTTTTCAAAATTCCATACAAATCTCTGCAAATTGCTTTTCCTGTCCCGTCGGTTCGTTCTGGATGCGATTCTCCATTTAGGTATGTTCATTTTCCCCCAAATCTGTTAGGTAAGGAAATGAATCTTCGAAACGACAAGCTATTTTCTCGTTTCTGAAATTTCGTTTGATCTCTGTCTGATTCTTGGAGATCCTGAAACCTAACCATGGAGGAAGAGGATAGTATTTACACGCAAGATGGAACGGTGGACTACCGTGGAGATCCTGCGATTAGAAGCCAAACAGGAACCTGGAAGGCCTGTCCCTATATTTTGGGTAGTTTTTCTGTTCTGGAAAAGTTAGGTCGATGAGTTTTCATATGGAATTGAACTGATGATTTCTATGACTTCTGAAATTTACTTGTTGATTCTTGAATGAATAGGCAATGAATTTTGTGAGAGATTGGCTTACTATGGAATGAGCACGAACTTGGTGCTCTACTTTAAGAAACATTTGAATCAGCACAGTGCTACCGCTGTTAAGAATGTGGCTAATTGGAGTGGAACCTGCTACATTACTCCCTTGATTGGAGCTTTTCTGGCTGATGCCTACTTGGGAAGATATCGAACTATTGCTACCTTCTCCATCATCTATGTTATCGTGAGTTCCTCTCTCCAAAACTTGTGGATTGCTACCTTCTCTATCATCTATGTTCTTGTGAGTTTTCTCTTGGAAACTTGTAGAAGATAGAGATCTATTGATACGATCAAATTTGATCGTGTAGCTCAACTAAGTTAAAACATATACTCTAAACTAAGAAGTGAATTCGAATCTAACTCCGGAATTGGTGTTTGAGAGTTTAAGCTTCAATGGGAAATTGTGATATATTTGCAGGGGATGACACTTTTGACATTGTCAGCTTCAGTTCATGGTTTGAAGCCCACATGTGTTGCTAAAGATGATTGTCAAGCGACGACTGCACAAAGTGCGGTGTGTTTTGTGGCTTTGTACTTGATTGCATTGGGAACTGGTGGGATTAAGCCTTGTGTTTCGTCTTACGGAGCTGATCAATTTGATGATGCCAATGAAACTGAGAAGAAGCACAAGAGCTCTTTCTTTAATTGGTTCTATCTTTCAATCAATGTTGGTGCTCTTATAGCAAGTTCTGTGCTTGTTTGGGTGCAAGATAATGTGAGTTGGGCTTGGGGGTTTGGTATTCCAGCCATAGCTATGGCGATTGCGGTGGCAAGTTTCTTTTCGGGGACTCGGTTGTTTCGGAACCAGAAACCTGGAGGAAGTCCTTTTACACGGATATCTCAGGTCATGGTGGCATCGTTTAGAAAATATAAGGTGAAAGTACCTGAAAGCAAGGCTTTGTACGAGACTGCTGATTCTGAGTCTTCTATCGTAGGAAGCCGCAAACTCGACCACACCGATGATTTCAGGTTCTTTGACAAGGCAGCAGTGGAGATCGAATCAGATCAGATGTTAAAGGGCTCAGTAAATCCGTGGAGGCTTTGCACTGTCACTCAAGTGGAGGAGCTAAAGGCCATCATAAGGTTGCTTCCAGTTTGGGCAACTGGGATTGTATTTGCTGCTGTATATAGTCAGATGGGCAATTTATTTGTGTTGCAAGGTGATAAAATGGATGCTCATATTGGCCCCAACTTTGAGATCCCAGCAGCATCTCTATCCATTTTCGACACTTTGAGCGTGATCTTTTGGGTCCCGATTTATGATCGAATCATAGTTCCAGTGGCTCGAAAATACACAGGTCATCCAAACGGTATAACTCAACTGCAGAGAATGGGCATTGGCCTATTTATTTCAATTCTAGCTATGCTATCTGCAGCAATCTTAGAGCTTGTGAGACTTCGAGAAGTGAGAAAGAACGACTATTATCTACTCGAAAAGATGCCAATGTCCATCTTCTGGCAAGTTCCTCAGTATTTTCTTATTGGTTGTGCTGAAGTATTTACCTTAATAGGCCAATTGGAGTTCTTTTACGAGCAAGCTCCCGACGCCATGAGAAGTCTCTGCTCCGCTCTCTCGCTTACTACCATTGCACTTGGAAACTACCTAAGCTCTCTACTCGTGACTATCGTCACCAAAGTCAGCACTAAAGATGGGAGTCTTGGATGGATACCAGACAACTTGAACTACGGTCATATACAATATTTCTTCTTTCTCTTGGTGATCTTGAGTATCAAAAATTTGATTGCTTTCCTTTTCATAGCCAAGTGGTACAAGTATAAGAGACCCATTGGAACAGTTCGCTGA

Coding sequence (CDS)

ATGACACTTTTGACATTGTCAGCTTCAGTTCATGGTTTGAAGCCCACATGTGTTGCTAAAGATGATTGTCAAGCGACGACTGCACAAAGTGCGGTGTGTTTTGTGGCTTTGTACTTGATTGCATTGGGAACTGGTGGGATTAAGCCTTGTGTTTCGTCTTACGGAGCTGATCAATTTGATGATGCCAATGAAACTGAGAAGAAGCACAAGAGCTCTTTCTTTAATTGGTTCTATCTTTCAATCAATGTTGGTGCTCTTATAGCAAGTTCTGTGCTTGTTTGGGTGCAAGATAATGTGAGTTGGGCTTGGGGGTTTGGTATTCCAGCCATAGCTATGGCGATTGCGGTGGCAAGTTTCTTTTCGGGGACTCGGTTGTTTCGGAACCAGAAACCTGGAGGAAGTCCTTTTACACGGATATCTCAGGTCATGGTGGCATCGTTTAGAAAATATAAGGTGAAAGTACCTGAAAGCAAGGCTTTGTACGAGACTGCTGATTCTGAGTCTTCTATCGTAGGAAGCCGCAAACTCGACCACACCGATGATTTCAGGTTCTTTGACAAGGCAGCAGTGGAGATCGAATCAGATCAGATGTTAAAGGGCTCAGTAAATCCGTGGAGGCTTTGCACTGTCACTCAAGTGGAGGAGCTAAAGGCCATCATAAGGTTGCTTCCAGTTTGGGCAACTGGGATTGTATTTGCTGCTGTATATAGTCAGATGGGCAATTTATTTGTGTTGCAAGGTGATAAAATGGATGCTCATATTGGCCCCAACTTTGAGATCCCAGCAGCATCTCTATCCATTTTCGACACTTTGAGCGTGATCTTTTGGGTCCCGATTTATGATCGAATCATAGTTCCAGTGGCTCGAAAATACACAGGTCATCCAAACGGTATAACTCAACTGCAGAGAATGGGCATTGGCCTATTTATTTCAATTCTAGCTATGCTATCTGCAGCAATCTTAGAGCTTGTGAGACTTCGAGAAGTGAGAAAGAACGACTATTATCTACTCGAAAAGATGCCAATGTCCATCTTCTGGCAAGTTCCTCAGTATTTTCTTATTGGTTGTGCTGAAGTATTTACCTTAATAGGCCAATTGGAGTTCTTTTACGAGCAAGCTCCCGACGCCATGAGAAGTCTCTGCTCCGCTCTCTCGCTTACTACCATTGCACTTGGAAACTACCTAAGCTCTCTACTCGTGACTATCGTCACCAAAGTCAGCACTAAAGATGGGAGTCTTGGATGGATACCAGACAACTTGAACTACGGTCATATACAATATTTCTTCTTTCTCTTGGTGATCTTGAGTATCAAAAATTTGATTGCTTTCCTTTTCATAGCCAAGTGGTACAAGTATAAGAGACCCATTGGAACAGTTCGCTGA

Protein sequence

MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFFSGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTDDFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQLQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVFTLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNLNYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR
Homology
BLAST of Carg23839 vs. NCBI nr
Match: KAG7035376.1 (Protein NRT1/ PTR FAMILY 8.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 909.8 bits (2350), Expect = 9.3e-261
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD
Sbjct: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF
Sbjct: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 180
           SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD
Sbjct: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 180

Query: 181 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 240
           DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG
Sbjct: 181 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 240

Query: 241 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 300
           NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ
Sbjct: 241 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 300

Query: 301 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 360
           LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF
Sbjct: 301 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 360

Query: 361 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 420
           TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL
Sbjct: 361 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 420

Query: 421 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 461
           NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR
Sbjct: 421 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 460

BLAST of Carg23839 vs. NCBI nr
Match: XP_023007552.1 (protein NRT1/ PTR FAMILY 8.1-like [Cucurbita maxima] >XP_023007553.1 protein NRT1/ PTR FAMILY 8.1-like [Cucurbita maxima])

HSP 1 Score: 907.5 bits (2344), Expect = 4.6e-260
Identity = 458/460 (99.57%), Postives = 459/460 (99.78%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD
Sbjct: 111 MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 170

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF
Sbjct: 171 DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 230

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 180
           SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD
Sbjct: 231 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 290

Query: 181 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 240
           DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG
Sbjct: 291 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 350

Query: 241 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 300
           NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ
Sbjct: 351 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 410

Query: 301 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 360
           LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF
Sbjct: 411 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 470

Query: 361 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 420
           TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTK+STKDG LGWIPDNL
Sbjct: 471 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKISTKDGRLGWIPDNL 530

Query: 421 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 461
           NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR
Sbjct: 531 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 570

BLAST of Carg23839 vs. NCBI nr
Match: KAG6605427.1 (Protein NRT1/ PTR FAMILY 8.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 907.1 bits (2343), Expect = 6.0e-260
Identity = 459/460 (99.78%), Postives = 459/460 (99.78%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASVHGLKPTCVAKD CQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD
Sbjct: 111 MTLLTLSASVHGLKPTCVAKDGCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 170

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF
Sbjct: 171 DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 230

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 180
           SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD
Sbjct: 231 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 290

Query: 181 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 240
           DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG
Sbjct: 291 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 350

Query: 241 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 300
           NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ
Sbjct: 351 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 410

Query: 301 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 360
           LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF
Sbjct: 411 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 470

Query: 361 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 420
           TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL
Sbjct: 471 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 530

Query: 421 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 461
           NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR
Sbjct: 531 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 570

BLAST of Carg23839 vs. NCBI nr
Match: XP_023532202.1 (protein NRT1/ PTR FAMILY 8.1-like [Cucurbita pepo subsp. pepo] >XP_023532204.1 protein NRT1/ PTR FAMILY 8.1-like [Cucurbita pepo subsp. pepo] >XP_023532205.1 protein NRT1/ PTR FAMILY 8.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 906.4 bits (2341), Expect = 1.0e-259
Identity = 458/460 (99.57%), Postives = 459/460 (99.78%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD
Sbjct: 111 MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 170

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF
Sbjct: 171 DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 230

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 180
           SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD
Sbjct: 231 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 290

Query: 181 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 240
           DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG
Sbjct: 291 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 350

Query: 241 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 300
           NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARK+TGHPNGITQ
Sbjct: 351 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKFTGHPNGITQ 410

Query: 301 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 360
           LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF
Sbjct: 411 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 470

Query: 361 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 420
           TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDG LGWIPDNL
Sbjct: 471 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGRLGWIPDNL 530

Query: 421 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 461
           NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR
Sbjct: 531 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 570

BLAST of Carg23839 vs. NCBI nr
Match: XP_022948150.1 (protein NRT1/ PTR FAMILY 8.1-like [Cucurbita moschata])

HSP 1 Score: 904.4 bits (2336), Expect = 3.9e-259
Identity = 457/460 (99.35%), Postives = 458/460 (99.57%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD
Sbjct: 111 MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 170

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF
Sbjct: 171 DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 230

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 180
           SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD
Sbjct: 231 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 290

Query: 181 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 240
           DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGI+FAAVYSQMG
Sbjct: 291 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGILFAAVYSQMG 350

Query: 241 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 300
           NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPN ITQ
Sbjct: 351 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNSITQ 410

Query: 301 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 360
           LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF
Sbjct: 411 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 470

Query: 361 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 420
           TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDG LGWIPDNL
Sbjct: 471 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGRLGWIPDNL 530

Query: 421 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 461
           NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR
Sbjct: 531 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 570

BLAST of Carg23839 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 5.8e-181
Identity = 311/458 (67.90%), Postives = 378/458 (82.53%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASV GLKP     D C   ++Q+AV FVALY+IALGTGGIKPCVSS+GADQFD
Sbjct: 110 MTLLTLSASVPGLKPGNCNADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFD 169

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           + +E EK  KSSFFNWFY SINVGALIA++VLVW+Q NV W WGFG+P +AM IAV  FF
Sbjct: 170 ENDENEKIKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFF 229

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKA-LYETADSESSIVGSRKLDHT 180
            G+R +R Q+PGGSP TRI QV+VA+FRK  VKVPE K+ L+ETAD ES+I GSRKL HT
Sbjct: 230 FGSRFYRLQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHT 289

Query: 181 DDFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQM 240
           D+ +FFDKAAVE +SD +  G VNPWRLC+VTQVEELK+II LLPVWATGIVFA VYSQM
Sbjct: 290 DNLKFFDKAAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQM 349

Query: 241 GNLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGIT 300
             +FVLQG+ MD H+G NFEIP+ASLS+FDT+SV+FW P+YD+ I+P+ARK+T +  G T
Sbjct: 350 STMFVLQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFT 409

Query: 301 QLQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEV 360
           QLQRMGIGL +SI AM++A +LE+VRL  V+ ++ Y  +++ MSIFWQ+PQY LIGCAEV
Sbjct: 410 QLQRMGIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEV 469

Query: 361 FTLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDN 420
           FT IGQLEFFY+QAPDAMRSLCSALSLTT+ALGNYLS++LVT+V K++ K+G  GWIPDN
Sbjct: 470 FTFIGQLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDN 529

Query: 421 LNYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIG 458
           LN GH+ YFF+LL  LS  N + +L+I+K YKYK+ +G
Sbjct: 530 LNRGHLDYFFYLLATLSFLNFLVYLWISKRYKYKKAVG 567

BLAST of Carg23839 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 1.3e-180
Identity = 312/458 (68.12%), Postives = 372/458 (81.22%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLT+SASV GL PTC + + C AT  Q+A+ F+ALYLIALGTGGIKPCVSS+GADQFD
Sbjct: 111 MTLLTISASVPGLTPTC-SGETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFD 170

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           D +E EK+ KSSFFNWFY  INVGA+IASSVLVW+Q NV W WG G+P +AMAIAV  FF
Sbjct: 171 DTDEKEKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFF 230

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKA-LYETADSESSIVGSRKLDHT 180
           +G+  +R QKPGGSP TR+ QV+VAS RK KVK+PE ++ LYE  D+ESSI+GSRKL+HT
Sbjct: 231 AGSNFYRLQKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHT 290

Query: 181 DDFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQM 240
               FFDKAAVE ESD       + W+LCTVTQVEELKA+IRLLP+WATGIVFA+VYSQM
Sbjct: 291 KILTFFDKAAVETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQM 350

Query: 241 GNLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGIT 300
           G +FVLQG+ +D H+GPNF+IP+ASLS+FDTLSV+FW P+YD++IVP ARKYTGH  G T
Sbjct: 351 GTVFVLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFT 410

Query: 301 QLQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEV 360
           QLQR+GIGL ISI +M+SA ILE+ RL  V+ ++ Y  E +PM+IFWQVPQYFL+GCAEV
Sbjct: 411 QLQRIGIGLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEV 470

Query: 361 FTLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDN 420
           FT IGQLEFFY+QAPDAMRSLCSALSLT IA GNYLS+ LVT+VTKV+   G  GWI  N
Sbjct: 471 FTFIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKN 530

Query: 421 LNYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIG 458
           LN GH+ YFF+LL  LS  N + +L+IAKWY YK+  G
Sbjct: 531 LNNGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYKKTTG 567

BLAST of Carg23839 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.3e-153
Identity = 278/459 (60.57%), Postives = 348/459 (75.82%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDC-QATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQF 60
           M+ LTLSASV  LKP     D C  AT AQ A+ F  LYLIALGTGGIKPCVSS+GADQF
Sbjct: 127 MSALTLSASVPALKPAECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQF 186

Query: 61  DDANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASF 120
           DD +  E+  K+SFFNWFY SIN+GAL++SS+LVW+Q+N  W  GFGIP + M +A+ASF
Sbjct: 187 DDTDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASF 246

Query: 121 FSGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPE-SKALYETADSESSIVGSRKLDH 180
           F GT L+R QKPGGSP TRISQV+VASFRK  VKVPE +  LYET D  S+I GSRK++H
Sbjct: 247 FFGTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEH 306

Query: 181 TDDFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQ 240
           TDD ++ DKAAV  E +       N WRLCTVTQVEELK +IR+ P+WA+GI+F+AVY+Q
Sbjct: 307 TDDCQYLDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQ 366

Query: 241 MGNLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGI 300
           M  +FV QG  M+  IG +F++P A+L  FDT SVI WVP+YDR IVP+ARK+TG   G 
Sbjct: 367 MSTMFVQQGRAMNCKIG-SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGF 426

Query: 301 TQLQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEK---MPMSIFWQVPQYFLIG 360
           T++QRMGIGLF+S+L M +AAI+E++RL     ND  L+E    +P+S+ WQ+PQYF++G
Sbjct: 427 TEIQRMGIGLFVSVLCMAAAAIVEIIRLH--MANDLGLVESGAPVPISVLWQIPQYFILG 486

Query: 361 CAEVFTLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGW 420
            AEVF  IGQLEFFY+Q+PDAMRSLCSAL+L T ALGNYLSSL++T+VT  +T++G  GW
Sbjct: 487 AAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGW 546

Query: 421 IPDNLNYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKR 455
           I DNLN GH+ YFF+LL  LS+ N+  + F A  YK K+
Sbjct: 547 ISDNLNSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQKK 582

BLAST of Carg23839 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 1.0e-137
Identity = 252/461 (54.66%), Postives = 333/461 (72.23%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTC---VAKDDCQ-ATTAQSAVCFVALYLIALGTGGIKPCVSSYGA 60
           M LLTLSAS+  LKP     VA   C  ATT Q AV F  LYLIALGTGGIKPCVSS+GA
Sbjct: 138 MALLTLSASLPVLKPAACAGVAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGA 197

Query: 61  DQFDDANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAV 120
           DQFDD +  E+  K+SFFNWFY SIN+G+ I+S++LVWVQ+NV W  GF IP + M +++
Sbjct: 198 DQFDDTDPRERVRKASFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSI 257

Query: 121 ASFFSGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPES-KALYETADSESSIVGSRK 180
           ASFF GT L+R QKPGGSP TR+ QV+VA++RK K+ +PE    LYET +  S I GSRK
Sbjct: 258 ASFFIGTPLYRFQKPGGSPITRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRK 317

Query: 181 LDHTDDFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAV 240
           + HTD ++F DKAAV  E +       NPW+LCTVTQVEE+K +IR+ P+WA+GIV++ +
Sbjct: 318 IQHTDGYKFLDKAAVISEYESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVL 377

Query: 241 YSQMGNLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHP 300
           YSQ+  LFV QG  M+  I  +FEIP AS  +FDTL V+  +PIYDR +VP  R++TG P
Sbjct: 378 YSQISTLFVQQGRSMN-RIIRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIP 437

Query: 301 NGITQLQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIG 360
            G+T LQRMGIGLF+S+L++ +AAI+E VRL+  +       + + MSIFWQ+PQY L+G
Sbjct: 438 KGLTDLQRMGIGLFLSVLSIAAAAIVETVRLQLAQ-------DFVAMSIFWQIPQYILMG 497

Query: 361 CAEVFTLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGW 420
            AEVF  IG++EFFY+++PDAMRS+CSAL+L   A+G+YLSSL++T+V   +   G  GW
Sbjct: 498 IAEVFFFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGW 557

Query: 421 IPDNLNYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPI 457
           +PD+LN GH+ YFF+LLV L + N+  +  I   +  K+ +
Sbjct: 558 VPDDLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTKKKAL 590

BLAST of Carg23839 vs. ExPASy Swiss-Prot
Match: Q84WG0 (Protein NRT1/ PTR FAMILY 8.4 OS=Arabidopsis thaliana OX=3702 GN=NPF8.4 PE=2 SV=2)

HSP 1 Score: 437.2 bits (1123), Expect = 2.3e-121
Identity = 240/459 (52.29%), Postives = 312/459 (67.97%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPT-CVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQF 60
           M  LTLSASV GLKP  C+      AT  QS V F  LYLIALGTGGIKPCVSS+GADQF
Sbjct: 128 MVALTLSASVPGLKPAECIGSLCPPATMVQSTVLFSGLYLIALGTGGIKPCVSSFGADQF 187

Query: 61  DDANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASF 120
           D  + +E+  K+SFFNWFY +IN+GA ++S+VLVW+Q+N  W  GF IP + M +A  SF
Sbjct: 188 DKTDPSERVRKASFFNWFYFTINIGAFVSSTVLVWIQENYGWELGFLIPTVFMGLATMSF 247

Query: 121 FSGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHT 180
           F GT L+R QKP GSP T + QV+VA++RK  +KVPE                    D T
Sbjct: 248 FFGTPLYRFQKPRGSPITSVCQVLVAAYRKSNLKVPE--------------------DST 307

Query: 181 DDFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQM 240
           D            E D     + NPW+LCTVTQVEE+K ++RL+P+WA+GI+F+ ++SQ+
Sbjct: 308 D------------EGD----ANTNPWKLCTVTQVEEVKILLRLVPIWASGIIFSVLHSQI 367

Query: 241 GNLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGIT 300
             LFV QG  M   IG  FEIP A+L +FDT SV+  VPIYDR+IVP+ R++TG   G T
Sbjct: 368 YTLFVQQGRCMKRTIG-LFEIPPATLGMFDTASVLISVPIYDRVIVPLVRRFTGLAKGFT 427

Query: 301 QLQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEK---MPMSIFWQVPQYFLIGC 360
           +LQRMGIGLF+S+L++  AAI+E VRL+  R  D  L+E    +P++IFWQ+PQYFL+G 
Sbjct: 428 ELQRMGIGLFVSVLSLTFAAIVETVRLQLAR--DLDLVESGDIVPLNIFWQIPQYFLMGT 487

Query: 361 AEVFTLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWI 420
           A VF  +G++EFFYEQ+PD+MRSLCSA +L T  LGNYLSSL++T+V  +S KD    WI
Sbjct: 488 AGVFFFVGRIEFFYEQSPDSMRSLCSAWALLTTTLGNYLSSLIITLVAYLSGKD---CWI 544

Query: 421 P-DNLNYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKR 455
           P DN+N GH+ YFF+LLV L   N+  F+F +  Y + +
Sbjct: 548 PSDNINNGHLDYFFWLLVSLGSVNIPVFVFFSVKYTHMK 544

BLAST of Carg23839 vs. ExPASy TrEMBL
Match: A0A6J1KZ11 (protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita maxima OX=3661 GN=LOC111500013 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.2e-260
Identity = 458/460 (99.57%), Postives = 459/460 (99.78%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD
Sbjct: 111 MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 170

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF
Sbjct: 171 DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 230

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 180
           SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD
Sbjct: 231 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 290

Query: 181 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 240
           DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG
Sbjct: 291 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 350

Query: 241 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 300
           NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ
Sbjct: 351 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 410

Query: 301 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 360
           LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF
Sbjct: 411 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 470

Query: 361 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 420
           TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTK+STKDG LGWIPDNL
Sbjct: 471 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKISTKDGRLGWIPDNL 530

Query: 421 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 461
           NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR
Sbjct: 531 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 570

BLAST of Carg23839 vs. ExPASy TrEMBL
Match: A0A6J1G8X9 (protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita moschata OX=3662 GN=LOC111451816 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 1.9e-259
Identity = 457/460 (99.35%), Postives = 458/460 (99.57%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD
Sbjct: 111 MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 170

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF
Sbjct: 171 DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 230

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 180
           SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD
Sbjct: 231 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 290

Query: 181 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 240
           DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGI+FAAVYSQMG
Sbjct: 291 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGILFAAVYSQMG 350

Query: 241 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 300
           NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPN ITQ
Sbjct: 351 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNSITQ 410

Query: 301 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 360
           LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF
Sbjct: 411 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 470

Query: 361 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 420
           TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDG LGWIPDNL
Sbjct: 471 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGRLGWIPDNL 530

Query: 421 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 461
           NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR
Sbjct: 531 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 570

BLAST of Carg23839 vs. ExPASy TrEMBL
Match: A0A6J1J8M5 (protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita maxima OX=3661 GN=LOC111484451 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.2e-242
Identity = 421/460 (91.52%), Postives = 445/460 (96.74%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASV GLKPTCVAKDDC AT AQSAVCFVALYLIALGTGGIKPCVSSYGADQFD
Sbjct: 111 MTLLTLSASVPGLKPTCVAKDDCHATAAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 170

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           DA+ETEKKHKSSFFNWFYLSINVGALIASSVLVWVQ+NVSW WGFGIPA+AMAIAV SFF
Sbjct: 171 DADETEKKHKSSFFNWFYLSINVGALIASSVLVWVQENVSWGWGFGIPAMAMAIAVVSFF 230

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 180
           SGTRL+RNQKPGGSPFTRI QV+VASFRKY+VKVPE+KALYET D+ES++VGSRKLDHTD
Sbjct: 231 SGTRLYRNQKPGGSPFTRICQVVVASFRKYEVKVPENKALYETTDNESAVVGSRKLDHTD 290

Query: 181 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 240
           DFRFFDKAAVE+ESDQMLKGSVNPW+LCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG
Sbjct: 291 DFRFFDKAAVELESDQMLKGSVNPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 350

Query: 241 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 300
            LFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVP+YDRIIVP+ARKYTGHPNGITQ
Sbjct: 351 TLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPMARKYTGHPNGITQ 410

Query: 301 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 360
           LQRMGIGLFISI+AMLSAAILELVRLREVR+++YY L+ MPMSIFWQVPQYFLIGCAEVF
Sbjct: 411 LQRMGIGLFISIIAMLSAAILELVRLREVRRHNYYELDHMPMSIFWQVPQYFLIGCAEVF 470

Query: 361 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 420
           T IGQLEFFYEQAPDAMRSL SALSLTT+ALGNYLSSLLVTIVTK STKDGS GWIPDNL
Sbjct: 471 TFIGQLEFFYEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTKFSTKDGSPGWIPDNL 530

Query: 421 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 461
           NYGHI YFFFLLVILS+KNLIA+LFIAKWYKYKRP+GT+R
Sbjct: 531 NYGHIHYFFFLLVILSVKNLIAYLFIAKWYKYKRPLGTLR 570

BLAST of Carg23839 vs. ExPASy TrEMBL
Match: A0A6J1D3U5 (protein NRT1/ PTR FAMILY 8.1-like OS=Momordica charantia OX=3673 GN=LOC111017019 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.6e-242
Identity = 420/460 (91.30%), Postives = 445/460 (96.74%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASV GLKPTCVAKDDC AT AQSAVCFVALYLIALGTGGIKPCVSSYGADQFD
Sbjct: 111 MTLLTLSASVPGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 170

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           DANETEK+HKSSFFNWFYLSINVGALIASSVLVWVQDNVSW WGFGIPA+AMAIAV SFF
Sbjct: 171 DANETEKRHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFF 230

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 180
           SGTRL+RNQKPGGSPFTRISQV+V+SFRKYKVKVPESK+LYETADSESSIVGSRKLDHTD
Sbjct: 231 SGTRLYRNQKPGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTD 290

Query: 181 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 240
           DFRFFDKAAVE+ESDQMLKGSV+PW+LCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG
Sbjct: 291 DFRFFDKAAVELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 350

Query: 241 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 300
           NLFVLQGD+M+ H+GPNFEIPAASLSIFDTLSVIFWVP+YDRIIVPVARKYTGHPNGITQ
Sbjct: 351 NLFVLQGDRMNIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQ 410

Query: 301 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 360
           LQRMGIGL ISILAMLSAAILELVRLREV++++YY L+ MPMSIFWQVPQYFLIGCAEVF
Sbjct: 411 LQRMGIGLLISILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVF 470

Query: 361 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 420
           T IGQLEFFYEQAPDAMRSL SALSLTT+ALGNYLSSLLVTIVT VSTK+G LGWIPDNL
Sbjct: 471 TFIGQLEFFYEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNL 530

Query: 421 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 461
           NYGHI YFFFLL +LS+KNLIAF+FIAKWYKYKRP+GT+R
Sbjct: 531 NYGHIHYFFFLLALLSVKNLIAFIFIAKWYKYKRPVGTLR 570

BLAST of Carg23839 vs. ExPASy TrEMBL
Match: A0A6J1FV98 (protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita moschata OX=3662 GN=LOC111448422 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.6e-242
Identity = 420/460 (91.30%), Postives = 447/460 (97.17%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASV GLKPTCVAKDDC AT AQSAVCFVALYLIALGTGGIKPCVSSYGADQFD
Sbjct: 111 MTLLTLSASVPGLKPTCVAKDDCHATAAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 170

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           DA+ETEKKHKSSFFNWFYLSINVGALIASSVLVWVQ+NVSW WGFGIPA+AMAIAV SFF
Sbjct: 171 DADETEKKHKSSFFNWFYLSINVGALIASSVLVWVQENVSWGWGFGIPAMAMAIAVVSFF 230

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHTD 180
           SGTRL+RNQKPGGSPFTRI QV+VASFRKY+VKVPE+KALYETAD+ES++VGSRKLDHTD
Sbjct: 231 SGTRLYRNQKPGGSPFTRICQVVVASFRKYEVKVPENKALYETADNESAVVGSRKLDHTD 290

Query: 181 DFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 240
           DFRFFDKAAVE+ESD+MLKG+VNPW+LCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG
Sbjct: 291 DFRFFDKAAVELESDEMLKGTVNPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG 350

Query: 241 NLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGITQ 300
            LFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVP+YDRIIVP+ARKYTGHPNGITQ
Sbjct: 351 TLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPMARKYTGHPNGITQ 410

Query: 301 LQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEVF 360
           LQRMGIGLFISI+AMLSAAILELVRL+EVR+++YY L+ MPMSIFWQVPQYFLIGCAEVF
Sbjct: 411 LQRMGIGLFISIIAMLSAAILELVRLQEVRRHNYYELDHMPMSIFWQVPQYFLIGCAEVF 470

Query: 361 TLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDNL 420
           T IGQLEFFYEQAPDAMRSL SALSLTT+ALGNYLSSLLVTIVTKVSTKDGS GWIPDNL
Sbjct: 471 TFIGQLEFFYEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTKVSTKDGSPGWIPDNL 530

Query: 421 NYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIGTVR 461
           NYGHI YFFFLLVILS+KNLIA+LFIAKWYKYKRP+GT+R
Sbjct: 531 NYGHIHYFFFLLVILSVKNLIAYLFIAKWYKYKRPLGTLR 570

BLAST of Carg23839 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 635.2 bits (1637), Expect = 4.1e-182
Identity = 311/458 (67.90%), Postives = 378/458 (82.53%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLTLSASV GLKP     D C   ++Q+AV FVALY+IALGTGGIKPCVSS+GADQFD
Sbjct: 110 MTLLTLSASVPGLKPGNCNADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFD 169

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           + +E EK  KSSFFNWFY SINVGALIA++VLVW+Q NV W WGFG+P +AM IAV  FF
Sbjct: 170 ENDENEKIKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFF 229

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKA-LYETADSESSIVGSRKLDHT 180
            G+R +R Q+PGGSP TRI QV+VA+FRK  VKVPE K+ L+ETAD ES+I GSRKL HT
Sbjct: 230 FGSRFYRLQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHT 289

Query: 181 DDFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQM 240
           D+ +FFDKAAVE +SD +  G VNPWRLC+VTQVEELK+II LLPVWATGIVFA VYSQM
Sbjct: 290 DNLKFFDKAAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQM 349

Query: 241 GNLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGIT 300
             +FVLQG+ MD H+G NFEIP+ASLS+FDT+SV+FW P+YD+ I+P+ARK+T +  G T
Sbjct: 350 STMFVLQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFT 409

Query: 301 QLQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEV 360
           QLQRMGIGL +SI AM++A +LE+VRL  V+ ++ Y  +++ MSIFWQ+PQY LIGCAEV
Sbjct: 410 QLQRMGIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEV 469

Query: 361 FTLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDN 420
           FT IGQLEFFY+QAPDAMRSLCSALSLTT+ALGNYLS++LVT+V K++ K+G  GWIPDN
Sbjct: 470 FTFIGQLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDN 529

Query: 421 LNYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIG 458
           LN GH+ YFF+LL  LS  N + +L+I+K YKYK+ +G
Sbjct: 530 LNRGHLDYFFYLLATLSFLNFLVYLWISKRYKYKKAVG 567

BLAST of Carg23839 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 634.0 bits (1634), Expect = 9.2e-182
Identity = 312/458 (68.12%), Postives = 372/458 (81.22%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQFD 60
           MTLLT+SASV GL PTC + + C AT  Q+A+ F+ALYLIALGTGGIKPCVSS+GADQFD
Sbjct: 111 MTLLTISASVPGLTPTC-SGETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFD 170

Query: 61  DANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASFF 120
           D +E EK+ KSSFFNWFY  INVGA+IASSVLVW+Q NV W WG G+P +AMAIAV  FF
Sbjct: 171 DTDEKEKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFF 230

Query: 121 SGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKA-LYETADSESSIVGSRKLDHT 180
           +G+  +R QKPGGSP TR+ QV+VAS RK KVK+PE ++ LYE  D+ESSI+GSRKL+HT
Sbjct: 231 AGSNFYRLQKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHT 290

Query: 181 DDFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQM 240
               FFDKAAVE ESD       + W+LCTVTQVEELKA+IRLLP+WATGIVFA+VYSQM
Sbjct: 291 KILTFFDKAAVETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQM 350

Query: 241 GNLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGIT 300
           G +FVLQG+ +D H+GPNF+IP+ASLS+FDTLSV+FW P+YD++IVP ARKYTGH  G T
Sbjct: 351 GTVFVLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFT 410

Query: 301 QLQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIGCAEV 360
           QLQR+GIGL ISI +M+SA ILE+ RL  V+ ++ Y  E +PM+IFWQVPQYFL+GCAEV
Sbjct: 411 QLQRIGIGLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEV 470

Query: 361 FTLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWIPDN 420
           FT IGQLEFFY+QAPDAMRSLCSALSLT IA GNYLS+ LVT+VTKV+   G  GWI  N
Sbjct: 471 FTFIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKN 530

Query: 421 LNYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPIG 458
           LN GH+ YFF+LL  LS  N + +L+IAKWY YK+  G
Sbjct: 531 LNNGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYKKTTG 567

BLAST of Carg23839 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 544.3 bits (1401), Expect = 9.6e-155
Identity = 278/459 (60.57%), Postives = 348/459 (75.82%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTCVAKDDC-QATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQF 60
           M+ LTLSASV  LKP     D C  AT AQ A+ F  LYLIALGTGGIKPCVSS+GADQF
Sbjct: 127 MSALTLSASVPALKPAECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQF 186

Query: 61  DDANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASF 120
           DD +  E+  K+SFFNWFY SIN+GAL++SS+LVW+Q+N  W  GFGIP + M +A+ASF
Sbjct: 187 DDTDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASF 246

Query: 121 FSGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPE-SKALYETADSESSIVGSRKLDH 180
           F GT L+R QKPGGSP TRISQV+VASFRK  VKVPE +  LYET D  S+I GSRK++H
Sbjct: 247 FFGTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEH 306

Query: 181 TDDFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQ 240
           TDD ++ DKAAV  E +       N WRLCTVTQVEELK +IR+ P+WA+GI+F+AVY+Q
Sbjct: 307 TDDCQYLDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQ 366

Query: 241 MGNLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGI 300
           M  +FV QG  M+  IG +F++P A+L  FDT SVI WVP+YDR IVP+ARK+TG   G 
Sbjct: 367 MSTMFVQQGRAMNCKIG-SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGF 426

Query: 301 TQLQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEK---MPMSIFWQVPQYFLIG 360
           T++QRMGIGLF+S+L M +AAI+E++RL     ND  L+E    +P+S+ WQ+PQYF++G
Sbjct: 427 TEIQRMGIGLFVSVLCMAAAAIVEIIRLH--MANDLGLVESGAPVPISVLWQIPQYFILG 486

Query: 361 CAEVFTLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGW 420
            AEVF  IGQLEFFY+Q+PDAMRSLCSAL+L T ALGNYLSSL++T+VT  +T++G  GW
Sbjct: 487 AAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGW 546

Query: 421 IPDNLNYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKR 455
           I DNLN GH+ YFF+LL  LS+ N+  + F A  YK K+
Sbjct: 547 ISDNLNSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQKK 582

BLAST of Carg23839 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 491.5 bits (1264), Expect = 7.4e-139
Identity = 252/461 (54.66%), Postives = 333/461 (72.23%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPTC---VAKDDCQ-ATTAQSAVCFVALYLIALGTGGIKPCVSSYGA 60
           M LLTLSAS+  LKP     VA   C  ATT Q AV F  LYLIALGTGGIKPCVSS+GA
Sbjct: 138 MALLTLSASLPVLKPAACAGVAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGA 197

Query: 61  DQFDDANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAV 120
           DQFDD +  E+  K+SFFNWFY SIN+G+ I+S++LVWVQ+NV W  GF IP + M +++
Sbjct: 198 DQFDDTDPRERVRKASFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSI 257

Query: 121 ASFFSGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPES-KALYETADSESSIVGSRK 180
           ASFF GT L+R QKPGGSP TR+ QV+VA++RK K+ +PE    LYET +  S I GSRK
Sbjct: 258 ASFFIGTPLYRFQKPGGSPITRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRK 317

Query: 181 LDHTDDFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAV 240
           + HTD ++F DKAAV  E +       NPW+LCTVTQVEE+K +IR+ P+WA+GIV++ +
Sbjct: 318 IQHTDGYKFLDKAAVISEYESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVL 377

Query: 241 YSQMGNLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHP 300
           YSQ+  LFV QG  M+  I  +FEIP AS  +FDTL V+  +PIYDR +VP  R++TG P
Sbjct: 378 YSQISTLFVQQGRSMN-RIIRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIP 437

Query: 301 NGITQLQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEKMPMSIFWQVPQYFLIG 360
            G+T LQRMGIGLF+S+L++ +AAI+E VRL+  +       + + MSIFWQ+PQY L+G
Sbjct: 438 KGLTDLQRMGIGLFLSVLSIAAAAIVETVRLQLAQ-------DFVAMSIFWQIPQYILMG 497

Query: 361 CAEVFTLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGW 420
            AEVF  IG++EFFY+++PDAMRS+CSAL+L   A+G+YLSSL++T+V   +   G  GW
Sbjct: 498 IAEVFFFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGW 557

Query: 421 IPDNLNYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKRPI 457
           +PD+LN GH+ YFF+LLV L + N+  +  I   +  K+ +
Sbjct: 558 VPDDLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTKKKAL 590

BLAST of Carg23839 vs. TAIR 10
Match: AT2G02020.1 (Major facilitator superfamily protein )

HSP 1 Score: 437.2 bits (1123), Expect = 1.6e-122
Identity = 240/459 (52.29%), Postives = 312/459 (67.97%), Query Frame = 0

Query: 1   MTLLTLSASVHGLKPT-CVAKDDCQATTAQSAVCFVALYLIALGTGGIKPCVSSYGADQF 60
           M  LTLSASV GLKP  C+      AT  QS V F  LYLIALGTGGIKPCVSS+GADQF
Sbjct: 128 MVALTLSASVPGLKPAECIGSLCPPATMVQSTVLFSGLYLIALGTGGIKPCVSSFGADQF 187

Query: 61  DDANETEKKHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWAWGFGIPAIAMAIAVASF 120
           D  + +E+  K+SFFNWFY +IN+GA ++S+VLVW+Q+N  W  GF IP + M +A  SF
Sbjct: 188 DKTDPSERVRKASFFNWFYFTINIGAFVSSTVLVWIQENYGWELGFLIPTVFMGLATMSF 247

Query: 121 FSGTRLFRNQKPGGSPFTRISQVMVASFRKYKVKVPESKALYETADSESSIVGSRKLDHT 180
           F GT L+R QKP GSP T + QV+VA++RK  +KVPE                    D T
Sbjct: 248 FFGTPLYRFQKPRGSPITSVCQVLVAAYRKSNLKVPE--------------------DST 307

Query: 181 DDFRFFDKAAVEIESDQMLKGSVNPWRLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQM 240
           D            E D     + NPW+LCTVTQVEE+K ++RL+P+WA+GI+F+ ++SQ+
Sbjct: 308 D------------EGD----ANTNPWKLCTVTQVEEVKILLRLVPIWASGIIFSVLHSQI 367

Query: 241 GNLFVLQGDKMDAHIGPNFEIPAASLSIFDTLSVIFWVPIYDRIIVPVARKYTGHPNGIT 300
             LFV QG  M   IG  FEIP A+L +FDT SV+  VPIYDR+IVP+ R++TG   G T
Sbjct: 368 YTLFVQQGRCMKRTIG-LFEIPPATLGMFDTASVLISVPIYDRVIVPLVRRFTGLAKGFT 427

Query: 301 QLQRMGIGLFISILAMLSAAILELVRLREVRKNDYYLLEK---MPMSIFWQVPQYFLIGC 360
           +LQRMGIGLF+S+L++  AAI+E VRL+  R  D  L+E    +P++IFWQ+PQYFL+G 
Sbjct: 428 ELQRMGIGLFVSVLSLTFAAIVETVRLQLAR--DLDLVESGDIVPLNIFWQIPQYFLMGT 487

Query: 361 AEVFTLIGQLEFFYEQAPDAMRSLCSALSLTTIALGNYLSSLLVTIVTKVSTKDGSLGWI 420
           A VF  +G++EFFYEQ+PD+MRSLCSA +L T  LGNYLSSL++T+V  +S KD    WI
Sbjct: 488 AGVFFFVGRIEFFYEQSPDSMRSLCSAWALLTTTLGNYLSSLIITLVAYLSGKD---CWI 544

Query: 421 P-DNLNYGHIQYFFFLLVILSIKNLIAFLFIAKWYKYKR 455
           P DN+N GH+ YFF+LLV L   N+  F+F +  Y + +
Sbjct: 548 PSDNINNGHLDYFFWLLVSLGSVNIPVFVFFSVKYTHMK 544

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035376.19.3e-261100.00Protein NRT1/ PTR FAMILY 8.1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023007552.14.6e-26099.57protein NRT1/ PTR FAMILY 8.1-like [Cucurbita maxima] >XP_023007553.1 protein NRT... [more]
KAG6605427.16.0e-26099.78Protein NRT1/ PTR FAMILY 8.1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023532202.11.0e-25999.57protein NRT1/ PTR FAMILY 8.1-like [Cucurbita pepo subsp. pepo] >XP_023532204.1 p... [more]
XP_022948150.13.9e-25999.35protein NRT1/ PTR FAMILY 8.1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M3905.8e-18167.90Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB81.3e-18068.12Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
P460321.3e-15360.57Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q93Z201.0e-13754.66Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Q84WG02.3e-12152.29Protein NRT1/ PTR FAMILY 8.4 OS=Arabidopsis thaliana OX=3702 GN=NPF8.4 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1KZ112.2e-26099.57protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita maxima OX=3661 GN=LOC111500013 PE... [more]
A0A6J1G8X91.9e-25999.35protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita moschata OX=3662 GN=LOC111451816 ... [more]
A0A6J1J8M51.2e-24291.52protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita maxima OX=3661 GN=LOC111484451 PE... [more]
A0A6J1D3U51.6e-24291.30protein NRT1/ PTR FAMILY 8.1-like OS=Momordica charantia OX=3673 GN=LOC111017019... [more]
A0A6J1FV981.6e-24291.30protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita moschata OX=3662 GN=LOC111448422 ... [more]
Match NameE-valueIdentityDescription
AT3G54140.14.1e-18267.90peptide transporter 1 [more]
AT5G01180.19.2e-18268.12peptide transporter 5 [more]
AT2G02040.19.6e-15560.57peptide transporter 2 [more]
AT1G62200.17.4e-13954.66Major facilitator superfamily protein [more]
AT2G02020.11.6e-12252.29Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 1..416
e-value: 2.0E-121
score: 405.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..457
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..456
e-value: 3.7E-164
score: 548.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 31..453
NoneNo IPR availablePANTHERPTHR11654:SF297PROTEIN NRT1/ PTR FAMILY 8.3coord: 1..457
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01023PTR2_2coord: 74..86

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23839-RACarg23839-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006857 oligopeptide transport
biological_process GO:0006817 phosphate ion transport
biological_process GO:0050896 response to stimulus
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity