Carg23785 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23785
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionglutathione S-transferase DHAR2-like
LocationCarg_Chr04: 1220319 .. 1224567 (-)
RNA-Seq ExpressionCarg23785
SyntenyCarg23785
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCTCGAAGCTGCGGTCAAGGCTGCCGTGGGTGCGCCCGATCTTATTGGGGACTGTATGTTCTTCTTCTTCCCCTTGCATCTTTACAATTTTGTTCGAGTTTTTTTGTGTCAATTTTGCGATTAGGGGTTGTTTGTTTTTGGGGGTATTGTAGGCCCTTTCAGCCATCGGGTCCTGTTAACTTTGGAGGAGAAGAAACTTTGCTACGAAGTGCATCTCATTAATTTATCCGACAAACCCAGTTGGTAATATCTTGAAACTTGGCTTCATTTTGGTTTCATATGGTGTTGTGTTGATTGTTAATGATTTTGGTAGGTTCTTGGAAGTGAGCCCCGGAGGGAAGGTGCCGGTGGTGAAGTTTGATGACAAATGGGTATCTGATTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTACCCTGAGCCTTCTCTTGTTTCTCCCCCTGAATTTTCTTCTGTGTAAGGCCTTTTTCCATTTGATTTAGTCCTAGTTCCATGTCTTAGTATGTTTCTTTTGTTCAGTAATGTTTTCAACAATTCAGATTAGAAGTACTAATCCAGATTAAGACAACTTAATCTGTAACTGGTTTGATTGAAGAATTAAAATCATGACACTATTTCTGAACTGTACAATTTCTCGTTTCACTACCTTATAATCCACGAAGCAAGCATTTGAATCATAATTTGGCTCATAGGGGATCAAAGATAAATGGTGCTTTCGTGAAAATGTTGAAGAGCAAGGACCCGAATGATGGTTCGGAGCTGGCTTTGCTTGAAGAGTTGAAGGCGTTGGATGAGCATCTTAAGGCTCATGTATGTGTGTTGAGTATATCAAACAACTATATTAAAGCATTTATGATTTTACTCCTCATTTTCGCCTATGCCATGCGGTTCCAGGGTCCTTATGTTGCTGGAGAGAAGGTCACTGCTGTTGATTTGAGTCTGGCACCAAAGTTGTACAATCTCGATATTGCTCTTGGCCATTTCAAGAAGTGGATATTCCCTGTAAACTTGAACAGTTTATCCAAATACAAAGAGGTATGATGTAGGCCTGATGTTATATAGTTTTGCCTTCTCTGTGCTTAGGCTTTGCTAGCTTCTTAGATCACCTGCAGTTTGAAATCTATTGTGTGCTGGTTGCTTTCAAGCTCAAGTAAACTCGAACCTGGGGTAATGGCGTGTATGTATGACTTATAGAGAATCCTAGGATAAACTATGTGCTTCGTTTAAGCGCAGACAGCCAGATTCAATGTAACTAATCTCACTAAACTTAGTTTGGTATGCATAAGTCAAATTTAAAAGTGACGAGTGGGTACGCGAGTTCATCTAGTTTCTTGGTGTTGAAAATTATTGGGATCAGTATTTGTCTTAGACCGAGCAAATAAAGAAGCGTGTTTTAAACAGTAAGAAAATAGGAAGAACAGAATGGACAGGGGGGGAGCAGCTTAGTGTGTGGAGAATTATGCAATGGATGCACAATATTATTCTGGTCAATTGTTGTTGTTTGGATTGATCTTGCAGCTGCTTTTCGCTCGAGAATCTTTCATGAAGACAAAGGTTGCCCCAGAACACGTAATTTCTGGTTGGGAGCCCAAGGTGAATGCTGCTGCAGAACCTGTAATTTCTGATAGTGAGCCCAAGGTGAATGCTGCTGCAGAACCTATAATTTCAGATAGTGAGCCCAAGGCGAATGCTGTCGCAGAACATGTAGTTTCTGATAGTGAGCCCAAGGTGAATGCTGCATGTACCAGGAGATGTTTTCATCCCAAGTTATGAAATTATTGGTGTTATAATCTTAAGCGTTGGACTGTCGTTGCTAGGCCATTGAGGTTGCTGTCAAGGCTGCTGTTGGTGCACTCGATGTTATTGGAGACTGTATGTTCTTCTTTCCCTTTCATCTTCTCATCAAAAATTTGTTTTGCTTTTGGGTGATTTTTTCCTTTCTTGCAGGCCCTTTCGCTCAGAGGGTTCTTTTGACTTTGGAGGAGAAGAAAGTTCCTTACAAATGGCATCTCATCGATTTATCCGACAAACCCAGTTGGTAATATCTCGAAACTTGGCTTGTATTTTAGTTCCAAAGAGGGTTTTAACTTTACGCAAACGGGGTTGTGTGGACTGTTAATGGTTTTGGCAGGTTCTTGGAAGTGAGCCCAGAAGGGAAGGTGCCGGTGGCCAAGATTGACGACAAATGGGTTCCCGACTCCGATGTCATTGTCGGGATTCTTGAGGAGAAGTATCCCAAGCCTATTCTTGCGACTCCACCCGAATTTTCTTCTGTGTAAGGCTTTTACTTTTTTGGTTTGATTTATTCCTCGTTCCATGGCTGATTATATTTCTTTTAACATATGAAGCTTGGGCCTTGTTTACTTTCCATCCACTTTTTGTTGAGTAACAATTTCAATAATTCAGATCTCTTTACTTTGGCTCATAGGGGATCAAAGATAAGTGGTGCTTTTGTAAAATTCTTGAAGAGTAAGGACCCGAACGATGGTTCGGAGCAGGCTTTGCTTGAAGAATTGAAGGCGTTGAACGAGCATCTCAATGCTCATGTATGCATGTCTAATATTTAGAACAACTATATTGCAGAGTTCATGATTTTACTCATTTCATATTTGGCAATGCTATATGGTACCAGGGTCCTTATGTTGCTGGAGAAAAGGTTACCGCTGTTGATTTGAGTCTGGCACCGAAGCTTTACCATCTTGATGTCGCACTTGGCCATTTCAAGAAGTGGATTTTCCCTGAAAACTTAACCAGTTTATCCAAATACAAAGAGGTACGATGTCGTTGCAGAGACGTTATATAGTTTGTGATTTATAATTCAATTTGATTTTTATAAACTAGCATCAAGCTTATGTTGAGGATCATTGAGAGTAGTACTCCGCATTGGTTAATTAAGGGGAATATCATGAGTTTATAAGTAAGGAACACTATCTCTATTGCTATGAGACATTTTGGAGAAACTAAAGCAAAACCACGAGGGTTTATGCTCAAAGTGGACAATATCATATTGTTGTGGAGAGTCGTGATTCATAACATGGTATCAGAGGCATGCTCTTAGCTTAGCCATGCTAATAGAATCCTCAAGTGTCGAACAAAGAAGTGGTGAGTCTCGAATGTGTAGTCAAAGGTGACTCAAGTGTCGAACAAAGGGTGTACTTTTTTCGAGGGCTCCAGAGAAGGATTCGAGTCTCGATTAAGGGGAGACTGTTCGAGAGCTCCAAGGAAGGAGGCCTCAAGGGGAGACTCTATGGTATACTTTATTCAGGGTTAAGCGTAGATGAAAAATTCAATGTAACTACTCCCACTAAACTAGTTTGTTGCACAAATGGAATCTAACCCTGATGAGTGGAATGCGAGTTTATCTGGTTTCTTGGAGTTTGAAATGGTCGGGATCACTGTGTGTTCAAACACAATGAAGCGTACAATGGATAGATACATTATATGATATGCTTTGATCATTTGTTATTGGTTGGATTGCTGATTTTGCAGTTGCTTTTCGCTCGAGAATCGTTTGTGAAGACGAAGGCTGCCCCAGAACATGTAATTTCTGGTTGGGAGGCCAAAGTGAATGCTGCATGAGCTGATCTGATGTTTTCGACCCGAGTTTATGATCCTAAGCTTGGAACTGTCGTTGCTATGTCCTATGCCTCATCATTAAGAGTGTCTATGTCTATGTTTAGGAATAACTCTGTTTCTTCTTCTCATTTGCATATTGATTATTCTTCCATCGACTAGAAACTTACAGTTACAGCTGCACCATTCTGGGAGAGCTTTGGTTAGTGAAATTTTTACTTTAAGCCGTAGAGGACTTCACAAAATGATGCTAATACTCCGATTCTCACTCAAATGATATGAGATCAAACCCGAAACTGAACCGAAAAGGTTCAACAATTAAGTATTGATTCAAAATCATTTGTATCAATTTAAGAAAAAAACAAGACCGGAATTGATTGTTTCATATCAAGAACACAGCTCTAACGCAAAAGTCTTAAATATGCTCGGTATGCTGAATCGACGTTGGCGCCTGTGAACTAGATTCGAGCAACGACCTTGAAAATGGATACATGTCGACGTATTAACTCAGGAACAAGCTATTATGGGTTAACTAAGATTTTTTCGAACTATTCAAATAAAACTATAATTTTATGAGCAGAGGCTGGAGAGATTCCGACACAGGTTGGACTTGCTGAGCTGAAAAAACTTCAGACGAGCTACAGCGATCTCCCTTTTTTCTACTTTTTCG

mRNA sequence

ATGGCCCTCGAAGCTGCGGTCAAGGCTGCCGTGGGTGCGCCCGATCTTATTGGGGACTGCCCTTTCAGCCATCGGGTCCTGTTAACTTTGGAGGAGAAGAAACTTTGCTACGAAGTGCATCTCATTAATTTATCCGACAAACCCAGTTGGTTCTTGGAAGTGAGCCCCGGAGGGAAGGTGCCGGTGGTGAAGTTTGATGACAAATGGGTATCTGATTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTACCCTGAGCCTTCTCTTGTTTCTCCCCCTGAATTTTCTTCTGTGGGATCAAAGATAAATGGTGCTTTCGTGAAAATGTTGAAGAGCAAGGACCCGAATGATGGTTCGGAGCTGGCTTTGCTTGAAGAGTTGAAGGCGTTGGATGAGCATCTTAAGGCTCATGTATGTGTGTTGAGTATATCAAACAACTATATTAAAGCATTTATGATTTTACTCCTCATTTTCGCCTATGCCATGCGGTTCCAGGGTCCTTATGTTGCTGGAGAGAAGGTCACTGCTGTTGATTTGAGTCTGGCACCAAAGTTGTACAATCTCGATATTGCTCTTGGCCATTTCAAGAAGTGGATATTCCCTGTAAACTTGAACAGTTTATCCAAATACAAAGAGCTGCTTTTCGCTCGAGAATCTTTCATGAAGACAAAGGTTGCCCCAGAACACGTAATTTCTGGTTGGGAGCCCAAGGTGAATGCTGCTGCAGAACCTGTAATTTCTGATAGTGAGCCCAAGGTGAATGCTGCTGCAGAACCTATAATTTCAGATAGTGAGCCCAAGGCGAATGCTGTCGCAGAACATGTAGTTTCTGATAGTGAGCCCAAGGCCATTGAGGTTGCTGTCAAGGCTGCTGTTGGTGCACTCGATGTTATTGGAGACTGCCCTTTCGCTCAGAGGGTTCTTTTGACTTTGGAGGAGAAGAAAGTTCCTTACAAATGGCATCTCATCGATTTATCCGACAAACCCAGTTGGTTCTTGGAAGTGAGCCCAGAAGGGAAGGTGCCGGTGGCCAAGATTGACGACAAATGGGTTCCCGACTCCGATGTCATTGTCGGGATTCTTGAGGAGAAGTATCCCAAGCCTATTCTTGCGACTCCACCCGAATTTTCTTCTGTGGGATCAAAGATAAGTGGTGCTTTTGTAAAATTCTTGAAGAGTAAGGACCCGAACGATGGTTCGGAGCAGGCTTTGCTTGAAGAATTGAAGGCGTTGAACGAGCATCTCAATGCTCATGGTCCTTATGTTGCTGGAGAAAAGGTTACCGCTGTTGATTTGAGTCTGGCACCGAAGCTTTACCATCTTGATGTCGCACTTGGCCATTTCAAGAAGTGGATTTTCCCTGAAAACTTAACCAGTTTATCCAAATACAAAGAGTTGCTTTTCGCTCGAGAATCGTTTGTGAAGACGAAGGCTGCCCCAGAACATGTAATTTCTGGTTGGGAGGCCAAAGTGAATGCTGCATGAGCTGATCTGATGTTTTCGACCCGAGTTTATGATCCTAAGCTTGGAACTGTCGTTGCTATGTCCTATGCCTCATCATTAAGAGTGTCTATGTCTATGTTTAGGAATAACTCTGTTTCTTCTTCTCATTTGCATATTGATTATTCTTCCATCGACTAGAAACTTACAGTTACAGCTGCACCATTCTGGGAGAGCTTTGGTTAGTGAAATTTTTACTTTAAGCCGTAGAGGACTTCACAAAATGATGCTAATACTCCGATTCTCACTCAAATGATATGAGATCAAACCCGAAACTGAACCGAAAAGGTTCAACAATTAAGTATTGATTCAAAATCATTTGTATCAATTTAAGAAAAAAACAAGACCGGAATTGATTGTTTCATATCAAGAACACAGCTCTAACGCAAAAGTCTTAAATATGCTCGGTATGCTGAATCGACGTTGGCGCCTGTGAACTAGATTCGAGCAACGACCTTGAAAATGGATACATGTCGACGTATTAACTCAGGAACAAGCTATTATGGGTTAACTAAGATTTTTTCGAACTATTCAAATAAAACTATAATTTTATGAGCAGAGGCTGGAGAGATTCCGACACAGGTTGGACTTGCTGAGCTGAAAAAACTTCAGACGAGCTACAGCGATCTCCCTTTTTTCTACTTTTTCG

Coding sequence (CDS)

ATGGCCCTCGAAGCTGCGGTCAAGGCTGCCGTGGGTGCGCCCGATCTTATTGGGGACTGCCCTTTCAGCCATCGGGTCCTGTTAACTTTGGAGGAGAAGAAACTTTGCTACGAAGTGCATCTCATTAATTTATCCGACAAACCCAGTTGGTTCTTGGAAGTGAGCCCCGGAGGGAAGGTGCCGGTGGTGAAGTTTGATGACAAATGGGTATCTGATTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTACCCTGAGCCTTCTCTTGTTTCTCCCCCTGAATTTTCTTCTGTGGGATCAAAGATAAATGGTGCTTTCGTGAAAATGTTGAAGAGCAAGGACCCGAATGATGGTTCGGAGCTGGCTTTGCTTGAAGAGTTGAAGGCGTTGGATGAGCATCTTAAGGCTCATGTATGTGTGTTGAGTATATCAAACAACTATATTAAAGCATTTATGATTTTACTCCTCATTTTCGCCTATGCCATGCGGTTCCAGGGTCCTTATGTTGCTGGAGAGAAGGTCACTGCTGTTGATTTGAGTCTGGCACCAAAGTTGTACAATCTCGATATTGCTCTTGGCCATTTCAAGAAGTGGATATTCCCTGTAAACTTGAACAGTTTATCCAAATACAAAGAGCTGCTTTTCGCTCGAGAATCTTTCATGAAGACAAAGGTTGCCCCAGAACACGTAATTTCTGGTTGGGAGCCCAAGGTGAATGCTGCTGCAGAACCTGTAATTTCTGATAGTGAGCCCAAGGTGAATGCTGCTGCAGAACCTATAATTTCAGATAGTGAGCCCAAGGCGAATGCTGTCGCAGAACATGTAGTTTCTGATAGTGAGCCCAAGGCCATTGAGGTTGCTGTCAAGGCTGCTGTTGGTGCACTCGATGTTATTGGAGACTGCCCTTTCGCTCAGAGGGTTCTTTTGACTTTGGAGGAGAAGAAAGTTCCTTACAAATGGCATCTCATCGATTTATCCGACAAACCCAGTTGGTTCTTGGAAGTGAGCCCAGAAGGGAAGGTGCCGGTGGCCAAGATTGACGACAAATGGGTTCCCGACTCCGATGTCATTGTCGGGATTCTTGAGGAGAAGTATCCCAAGCCTATTCTTGCGACTCCACCCGAATTTTCTTCTGTGGGATCAAAGATAAGTGGTGCTTTTGTAAAATTCTTGAAGAGTAAGGACCCGAACGATGGTTCGGAGCAGGCTTTGCTTGAAGAATTGAAGGCGTTGAACGAGCATCTCAATGCTCATGGTCCTTATGTTGCTGGAGAAAAGGTTACCGCTGTTGATTTGAGTCTGGCACCGAAGCTTTACCATCTTGATGTCGCACTTGGCCATTTCAAGAAGTGGATTTTCCCTGAAAACTTAACCAGTTTATCCAAATACAAAGAGTTGCTTTTCGCTCGAGAATCGTTTGTGAAGACGAAGGCTGCCCCAGAACATGTAATTTCTGGTTGGGAGGCCAAAGTGAATGCTGCATGA

Protein sequence

MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKVPVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSELALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLSLAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNAAAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGILEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAPEHVISGWEAKVNAA
Homology
BLAST of Carg23785 vs. NCBI nr
Match: KAG7030743.1 (Glutathione S-transferase DHAR2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 978.8 bits (2529), Expect = 1.8e-281
Identity = 494/494 (100.00%), Postives = 494/494 (100.00%), Query Frame = 0

Query: 1   MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV 60
           MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV
Sbjct: 1   MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV 60

Query: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120
           PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE
Sbjct: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120

Query: 121 LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS 180
           LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS
Sbjct: 121 LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS 180

Query: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 240
           LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 240

Query: 241 AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300
           AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD
Sbjct: 241 AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300

Query: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360
           CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI
Sbjct: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360

Query: 361 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 420
           LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP
Sbjct: 361 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 420

Query: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 480
           YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP
Sbjct: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 480

Query: 481 EHVISGWEAKVNAA 495
           EHVISGWEAKVNAA
Sbjct: 481 EHVISGWEAKVNAA 494

BLAST of Carg23785 vs. NCBI nr
Match: KAG6600074.1 (Glutathione S-transferase DHAR2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 890.6 bits (2300), Expect = 6.3e-255
Identity = 454/494 (91.90%), Postives = 461/494 (93.32%), Query Frame = 0

Query: 1   MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV 60
           MA+E AVKAAVGAP++IGDCPFS RVLLTLEEKK+ Y++HLINLSDKP WFLEVSP GKV
Sbjct: 219 MAIEVAVKAAVGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 278

Query: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120
           PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE
Sbjct: 279 PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 338

Query: 121 LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS 180
           LALLEELKALDEHLKAH                            GPYVAGEKVTAVDLS
Sbjct: 339 LALLEELKALDEHLKAH----------------------------GPYVAGEKVTAVDLS 398

Query: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 240
           LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA
Sbjct: 399 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 458

Query: 241 AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300
           AAEPVISDSEPKVNAAAEP+ISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD
Sbjct: 459 AAEPVISDSEPKVNAAAEPVISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 518

Query: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360
           CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI
Sbjct: 519 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 578

Query: 361 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 420
           LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP
Sbjct: 579 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 638

Query: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 480
           YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP
Sbjct: 639 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 684

Query: 481 EHVISGWEAKVNAA 495
           EHVISGWEAKVNAA
Sbjct: 699 EHVISGWEAKVNAA 684

BLAST of Carg23785 vs. NCBI nr
Match: XP_022943189.1 (uncharacterized protein LOC111447994 isoform X1 [Cucurbita moschata])

HSP 1 Score: 880.6 bits (2274), Expect = 6.5e-252
Identity = 448/494 (90.69%), Postives = 457/494 (92.51%), Query Frame = 0

Query: 1   MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV 60
           MA+E AVKAAVGAP++IGDCPFS RVLLTLEEKK+ Y++HLINLSDKP WFLEVSP GKV
Sbjct: 1   MAIEVAVKAAVGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120
           PVVKFDDKWVSDSDVIVGILEEKYPEPSLV+PPEFSSVGSK NGAFVKMLKSKDPNDGSE
Sbjct: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVTPPEFSSVGSKTNGAFVKMLKSKDPNDGSE 120

Query: 121 LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS 180
           LALLEELKALDEHLKAH                            GPYVAGEKVTAVDLS
Sbjct: 121 LALLEELKALDEHLKAH----------------------------GPYVAGEKVTAVDLS 180

Query: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 240
           LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTK APEHVISGWEPKVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 240

Query: 241 AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300
           AAEPVISDSEPKVNAAAEP+ISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD
Sbjct: 241 AAEPVISDSEPKVNAAAEPVISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300

Query: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360
           CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI
Sbjct: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360

Query: 361 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 420
           LEEKYP+P LATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHL AHGP
Sbjct: 361 LEEKYPEPFLATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLKAHGP 420

Query: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 480
           YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP
Sbjct: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 466

Query: 481 EHVISGWEAKVNAA 495
           EHVISGWEAKVNAA
Sbjct: 481 EHVISGWEAKVNAA 466

BLAST of Carg23785 vs. NCBI nr
Match: XP_023547263.1 (uncharacterized protein LOC111806131 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 865.9 bits (2236), Expect = 1.7e-247
Identity = 440/494 (89.07%), Postives = 451/494 (91.30%), Query Frame = 0

Query: 1   MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV 60
           MA+E AVKAAVGAP++IGDCPFS RVLLTLEEKK+ Y++HLINLSDKP WFLEVSP GKV
Sbjct: 1   MAIEVAVKAAVGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120
           PVVKFDDKWV DSDVIVGILEEKYPEPSLV+PPEFSSVGSKINGAFVKMLKSKDPNDGSE
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120

Query: 121 LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS 180
           LALLEELKALDEHLKAH                            GPYVAGEKVTAVDLS
Sbjct: 121 LALLEELKALDEHLKAH----------------------------GPYVAGEKVTAVDLS 180

Query: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 240
           LAPKLYNLDIALGHFKKWI PVNL SLSKYKELLFARESFMKTK APEHVISGWEPKVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWISPVNLTSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 240

Query: 241 AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300
            AEPVISDSEPKVNAAAEP+ISDSEPK NA AEHVVSDSEPKAIEVAVKAAVGALDVIGD
Sbjct: 241 TAEPVISDSEPKVNAAAEPVISDSEPKVNAAAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300

Query: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360
           CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI
Sbjct: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360

Query: 361 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 420
           LEEKYP+P LATPPEFS+VGSKI+GAFVKFLKSKDPNDGSEQALLEELKALNEHL AHGP
Sbjct: 361 LEEKYPEPFLATPPEFSTVGSKINGAFVKFLKSKDPNDGSEQALLEELKALNEHLKAHGP 420

Query: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 480
           YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP
Sbjct: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 466

Query: 481 EHVISGWEAKVNAA 495
           EHVISGWEAKV AA
Sbjct: 481 EHVISGWEAKVKAA 466

BLAST of Carg23785 vs. NCBI nr
Match: XP_022985936.1 (uncharacterized protein LOC111483829 isoform X1 [Cucurbita maxima])

HSP 1 Score: 864.0 bits (2231), Expect = 6.3e-247
Identity = 437/494 (88.46%), Postives = 452/494 (91.50%), Query Frame = 0

Query: 1   MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV 60
           MA+E AVKAA+GAP++IGDCPFS RVLLTLEEKK+ Y++HLINLSDKP WFLEVSP GKV
Sbjct: 1   MAIEVAVKAAIGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120
           PVVKFDDKWV DSDVIVGILEEKYPEPSLV+PPEFSSVGSKINGAFVKMLKSKDPNDGSE
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120

Query: 121 LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS 180
           LALLEELKALDEHLKAH                            GPYVAGEKVTAVDLS
Sbjct: 121 LALLEELKALDEHLKAH----------------------------GPYVAGEKVTAVDLS 180

Query: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 240
           LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTK APEHVISGWEPKVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 240

Query: 241 AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300
           AA PVISDSEPKVNAA EP++SDSEPKAN+VAEHVVSD EPKAIEVAVKAAVGALDVIGD
Sbjct: 241 AAVPVISDSEPKVNAATEPVVSDSEPKANSVAEHVVSDCEPKAIEVAVKAAVGALDVIGD 300

Query: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360
           CPFAQRVLLTLEEKKVPYKWHLIDLSDKP WFLEVSPEGKVPVAKIDD+WVPDSDVIVGI
Sbjct: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPCWFLEVSPEGKVPVAKIDDRWVPDSDVIVGI 360

Query: 361 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 420
           LEEKYP+P LATPPEFSSVGSKI+GAFVKFLKSKDP+DGSEQALLEELKALNEHL AHGP
Sbjct: 361 LEEKYPEPFLATPPEFSSVGSKINGAFVKFLKSKDPSDGSEQALLEELKALNEHLKAHGP 420

Query: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 480
           YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP
Sbjct: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 466

Query: 481 EHVISGWEAKVNAA 495
           EHVISGWEAKVN A
Sbjct: 481 EHVISGWEAKVNDA 466

BLAST of Carg23785 vs. ExPASy Swiss-Prot
Match: Q9FRL8 (Glutathione S-transferase DHAR2 OS=Arabidopsis thaliana OX=3702 GN=DHAR2 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 2.6e-86
Identity = 153/212 (72.17%), Postives = 182/212 (85.85%), Query Frame = 0

Query: 283 AIEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVP 342
           A+++ VK AVGA DV+GDCPF+QRVLLTLEEKK+PYK HLI++SDKP WFL++SPEGKVP
Sbjct: 2   ALDICVKVAVGAPDVLGDCPFSQRVLLTLEEKKLPYKTHLINVSDKPQWFLDISPEGKVP 61

Query: 343 VAKIDDKWVPDSDVIVGILEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQ 402
           V K+D KWV DSDVIVG+LEEKYP+P L TPPEF+SVGSKI GAFV FLKSKD NDGSE+
Sbjct: 62  VVKLDGKWVADSDVIVGLLEEKYPEPSLKTPPEFASVGSKIFGAFVTFLKSKDANDGSEK 121

Query: 403 ALLEELKALNEHLNAH-GPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLS 462
           AL++EL+AL  HL  H GP+VAGEK+TAVDLSLAPKLYHL+VALGH+K W  PE+LTS+ 
Sbjct: 122 ALVDELEALENHLKTHSGPFVAGEKITAVDLSLAPKLYHLEVALGHYKNWSVPESLTSVR 181

Query: 463 KYKELLFARESFVKTKAAPEHVISGWEAKVNA 494
            Y + LF+RESF  TKA  E V++GWE+KVNA
Sbjct: 182 NYAKALFSRESFENTKAKKEIVVAGWESKVNA 213

BLAST of Carg23785 vs. ExPASy Swiss-Prot
Match: Q65XA0 (Probable glutathione S-transferase DHAR1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=DHAR1 PE=1 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 4.8e-80
Identity = 143/211 (67.77%), Postives = 170/211 (80.57%), Query Frame = 0

Query: 284 IEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPV 343
           +EV VKAAVG  D +GDCPF+QRVLLTLEEKKVPY+  LID+ +KP WFL++SPEGKVPV
Sbjct: 3   VEVCVKAAVGHPDTLGDCPFSQRVLLTLEEKKVPYEMKLIDVQNKPDWFLKISPEGKVPV 62

Query: 344 AK-IDDKWVPDSDVIVGILEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQ 403
               D KW+PDSDVI  ++EEKYP P L TPPE++SVGSKI   F  FLKSKDPNDGSE+
Sbjct: 63  FNGGDGKWIPDSDVITQVIEEKYPTPSLVTPPEYASVGSKIFSCFTTFLKSKDPNDGSEK 122

Query: 404 ALLEELKALNEHLNAHGPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSK 463
           ALL EL+AL EHL AHGP++ G+ ++A DLSLAPKLYHL VAL HFK W  PE+LT++  
Sbjct: 123 ALLTELQALEEHLKAHGPFINGQNISAADLSLAPKLYHLQVALEHFKGWKIPEDLTNVHA 182

Query: 464 YKELLFARESFVKTKAAPEHVISGWEAKVNA 494
           Y E LF+RESF+KTKAA EH+I+GW  KVNA
Sbjct: 183 YTEALFSRESFIKTKAAKEHLIAGWAPKVNA 213

BLAST of Carg23785 vs. ExPASy Swiss-Prot
Match: Q9FWR4 (Glutathione S-transferase DHAR1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DHAR1 PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 5.8e-78
Identity = 144/211 (68.25%), Postives = 167/211 (79.15%), Query Frame = 0

Query: 283 AIEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVP 342
           A+E+ VKAAVGA D +GDCPF+QR LLTLEEK + YK HLI+LSDKP WFL++SP+GKVP
Sbjct: 2   ALEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQWFLDISPQGKVP 61

Query: 343 VAKIDDKWVPDSDVIVGILEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQ 402
           V KIDDKWV DSDVIVGILEEKYP P L TP EF+SVGS I G F  FLKSKD NDGSE 
Sbjct: 62  VLKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSEH 121

Query: 403 ALLEELKALNEHLNAH-GPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLS 462
           ALL EL+AL  HL +H GP++AGE+V+AVDLSLAPKLYHL VALGHFK W  PE+   + 
Sbjct: 122 ALLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHVH 181

Query: 463 KYKELLFARESFVKTKAAPEHVISGWEAKVN 493
            Y + LF+ +SF KTK   ++VISGW  KVN
Sbjct: 182 NYMKTLFSLDSFEKTKTEEKYVISGWAPKVN 212

BLAST of Carg23785 vs. ExPASy Swiss-Prot
Match: Q9FG59 (Probable glutathione S-transferase DHAR4 OS=Arabidopsis thaliana OX=3702 GN=DHAR4 PE=3 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.5e-73
Identity = 139/214 (64.95%), Postives = 171/214 (79.91%), Query Frame = 0

Query: 284 IEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPV 343
           IEV VKAA GA DV+GDCPF QR+LLTLE+KK+PYK HLID+S KP WFL +SP+GK+P+
Sbjct: 3   IEVCVKAASGAPDVLGDCPFGQRILLTLEDKKLPYKTHLIDVSLKPDWFLAISPKGKLPL 62

Query: 344 AKI--DDKWVPDSDVIVGILEEKYPKPILAT-PPEFSSVGSKISGAFVKFLKSKD-PNDG 403
            K   D+ WV DSD+IVGI+EEKYP+P L T PPEF+SVGSKI GAFV FL SKD  NDG
Sbjct: 63  VKFDEDENWVADSDLIVGIIEEKYPEPSLVTFPPEFASVGSKIIGAFVMFLTSKDHANDG 122

Query: 404 SEQALLEELKALNEHLNAH-GPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLT 463
           S+ ALL+EL+AL+ HL  H GP+VAG+KVT VDLSLAPKLYHL+  LGHF  W  PE+LT
Sbjct: 123 SDMALLDELEALDHHLKTHVGPFVAGDKVTVVDLSLAPKLYHLETTLGHFMDWCVPESLT 182

Query: 464 SLSKYKELLFARESFVKTKAAPEHVISGWEAKVN 493
           ++  Y ++LF+ ESF KTKAA E++I+ W  K++
Sbjct: 183 NVRDYMKVLFSLESFEKTKAAKEYLIASWAPKLD 216

BLAST of Carg23785 vs. ExPASy Swiss-Prot
Match: Q8LE52 (Glutathione S-transferase DHAR3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DHAR3 PE=1 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.0e-71
Identity = 126/225 (56.00%), Postives = 163/225 (72.44%), Query Frame = 0

Query: 267 KANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLS 326
           K   V   V   +    +E+ VKA++   + +GDCPF Q+VLLT+EEK VPY   ++DLS
Sbjct: 32  KPGRVGRFVTMATAASPLEICVKASITTPNKLGDCPFCQKVLLTMEEKNVPYDMKMVDLS 91

Query: 327 DKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGILEEKYPKPILATPPEFSSVGSKISGA 386
           +KP WFL++SPEGKVPV K D+KWVPDSDVI   LEEKYP+P LATPPE +SVGSKI   
Sbjct: 92  NKPEWFLKISPEGKVPVVKFDEKWVPDSDVITQALEEKYPEPPLATPPEKASVGSKIFST 151

Query: 387 FVKFLKSKDPNDGSEQALLEELKALNEHLNAHGPYVAGEKVTAVDLSLAPKLYHLDVALG 446
           FV FLKSKD  DG+EQ LL+EL   N+++  +GP++ GEK++A DLSLAPKLYH+ +ALG
Sbjct: 152 FVGFLKSKDSGDGTEQVLLDELTTFNDYIKDNGPFINGEKISAADLSLAPKLYHMKIALG 211

Query: 447 HFKKWIFPENLTSLSKYKELLFARESFVKTKAAPEHVISGWEAKV 492
           H+K W  P++L  +  Y E +F+RESF  T+A  E VI+GW  KV
Sbjct: 212 HYKNWSVPDSLPFVKSYMENVFSRESFTNTRAETEDVIAGWRPKV 256

BLAST of Carg23785 vs. ExPASy TrEMBL
Match: A0A6J1FTJ7 (uncharacterized protein LOC111447994 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447994 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.1e-252
Identity = 448/494 (90.69%), Postives = 457/494 (92.51%), Query Frame = 0

Query: 1   MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV 60
           MA+E AVKAAVGAP++IGDCPFS RVLLTLEEKK+ Y++HLINLSDKP WFLEVSP GKV
Sbjct: 1   MAIEVAVKAAVGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120
           PVVKFDDKWVSDSDVIVGILEEKYPEPSLV+PPEFSSVGSK NGAFVKMLKSKDPNDGSE
Sbjct: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVTPPEFSSVGSKTNGAFVKMLKSKDPNDGSE 120

Query: 121 LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS 180
           LALLEELKALDEHLKAH                            GPYVAGEKVTAVDLS
Sbjct: 121 LALLEELKALDEHLKAH----------------------------GPYVAGEKVTAVDLS 180

Query: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 240
           LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTK APEHVISGWEPKVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 240

Query: 241 AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300
           AAEPVISDSEPKVNAAAEP+ISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD
Sbjct: 241 AAEPVISDSEPKVNAAAEPVISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300

Query: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360
           CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI
Sbjct: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360

Query: 361 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 420
           LEEKYP+P LATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHL AHGP
Sbjct: 361 LEEKYPEPFLATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLKAHGP 420

Query: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 480
           YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP
Sbjct: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 466

Query: 481 EHVISGWEAKVNAA 495
           EHVISGWEAKVNAA
Sbjct: 481 EHVISGWEAKVNAA 466

BLAST of Carg23785 vs. ExPASy TrEMBL
Match: A0A6J1J9M2 (uncharacterized protein LOC111483829 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483829 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 3.0e-247
Identity = 437/494 (88.46%), Postives = 452/494 (91.50%), Query Frame = 0

Query: 1   MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV 60
           MA+E AVKAA+GAP++IGDCPFS RVLLTLEEKK+ Y++HLINLSDKP WFLEVSP GKV
Sbjct: 1   MAIEVAVKAAIGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120
           PVVKFDDKWV DSDVIVGILEEKYPEPSLV+PPEFSSVGSKINGAFVKMLKSKDPNDGSE
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120

Query: 121 LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS 180
           LALLEELKALDEHLKAH                            GPYVAGEKVTAVDLS
Sbjct: 121 LALLEELKALDEHLKAH----------------------------GPYVAGEKVTAVDLS 180

Query: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 240
           LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTK APEHVISGWEPKVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 240

Query: 241 AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300
           AA PVISDSEPKVNAA EP++SDSEPKAN+VAEHVVSD EPKAIEVAVKAAVGALDVIGD
Sbjct: 241 AAVPVISDSEPKVNAATEPVVSDSEPKANSVAEHVVSDCEPKAIEVAVKAAVGALDVIGD 300

Query: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360
           CPFAQRVLLTLEEKKVPYKWHLIDLSDKP WFLEVSPEGKVPVAKIDD+WVPDSDVIVGI
Sbjct: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPCWFLEVSPEGKVPVAKIDDRWVPDSDVIVGI 360

Query: 361 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 420
           LEEKYP+P LATPPEFSSVGSKI+GAFVKFLKSKDP+DGSEQALLEELKALNEHL AHGP
Sbjct: 361 LEEKYPEPFLATPPEFSSVGSKINGAFVKFLKSKDPSDGSEQALLEELKALNEHLKAHGP 420

Query: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 480
           YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP
Sbjct: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 466

Query: 481 EHVISGWEAKVNAA 495
           EHVISGWEAKVN A
Sbjct: 481 EHVISGWEAKVNDA 466

BLAST of Carg23785 vs. ExPASy TrEMBL
Match: A0A6J1FXI2 (probable glutathione S-transferase DHAR2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447994 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 4.3e-241
Identity = 433/494 (87.65%), Postives = 442/494 (89.47%), Query Frame = 0

Query: 1   MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV 60
           MA+E AVKAAVGAP++IGDCPFS RVLLTLEEKK+ Y++HLINLSDKP WFLEVSP GKV
Sbjct: 1   MAIEVAVKAAVGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120
           PVVKFDDKWVSDSDVIVGILEEKYPEPSLV+PPEFSSVGSK NGAFVKMLKSKDPNDGSE
Sbjct: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVTPPEFSSVGSKTNGAFVKMLKSKDPNDGSE 120

Query: 121 LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS 180
           LALLEELKALDEHLKAH                            GPYVAGEKVTAVDLS
Sbjct: 121 LALLEELKALDEHLKAH----------------------------GPYVAGEKVTAVDLS 180

Query: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 240
           LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTK APEHVISGWEPKVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 240

Query: 241 AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300
           AAEPVISDSEPKVNAAAEP               V+SDSEPKAIEVAVKAAVGALDVIGD
Sbjct: 241 AAEPVISDSEPKVNAAAEP---------------VISDSEPKAIEVAVKAAVGALDVIGD 300

Query: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360
           CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI
Sbjct: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360

Query: 361 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 420
           LEEKYP+P LATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHL AHGP
Sbjct: 361 LEEKYPEPFLATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLKAHGP 420

Query: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 480
           YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP
Sbjct: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 451

Query: 481 EHVISGWEAKVNAA 495
           EHVISGWEAKVNAA
Sbjct: 481 EHVISGWEAKVNAA 451

BLAST of Carg23785 vs. ExPASy TrEMBL
Match: A0A6J1JEQ3 (probable glutathione S-transferase DHAR2, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483829 PE=3 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 2.9e-237
Identity = 426/494 (86.23%), Postives = 438/494 (88.66%), Query Frame = 0

Query: 1   MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV 60
           MA+E AVKAA+GAP++IGDCPFS RVLLTLEEKK+ Y++HLINLSDKP WFLEVSP GKV
Sbjct: 1   MAIEVAVKAAIGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120
           PVVKFDDKWV DSDVIVGILEEKYPEPSLV+PPEFSSVGSKINGAFVKMLKSKDPNDGSE
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120

Query: 121 LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS 180
           LALLEELKALDEHLKAH                            GPYVAGEKVTAVDLS
Sbjct: 121 LALLEELKALDEHLKAH----------------------------GPYVAGEKVTAVDLS 180

Query: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 240
           LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTK APEHVISGWEPKVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 240

Query: 241 AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300
           AA PVISDSEPKVNAA EP               VVSDSEPKAIEVAVKAAVGALDVIGD
Sbjct: 241 AAVPVISDSEPKVNAATEP---------------VVSDSEPKAIEVAVKAAVGALDVIGD 300

Query: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360
           CPFAQRVLLTLEEKKVPYKWHLIDLSDKP WFLEVSPEGKVPVAKIDD+WVPDSDVIVGI
Sbjct: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPCWFLEVSPEGKVPVAKIDDRWVPDSDVIVGI 360

Query: 361 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 420
           LEEKYP+P LATPPEFSSVGSKI+GAFVKFLKSKDP+DGSEQALLEELKALNEHL AHGP
Sbjct: 361 LEEKYPEPFLATPPEFSSVGSKINGAFVKFLKSKDPSDGSEQALLEELKALNEHLKAHGP 420

Query: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 480
           YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP
Sbjct: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 451

Query: 481 EHVISGWEAKVNAA 495
           EHVISGWEAKVN A
Sbjct: 481 EHVISGWEAKVNDA 451

BLAST of Carg23785 vs. ExPASy TrEMBL
Match: A0A6J1FR13 (uncharacterized protein LOC111447994 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111447994 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 1.1e-231
Identity = 419/494 (84.82%), Postives = 427/494 (86.44%), Query Frame = 0

Query: 1   MALEAAVKAAVGAPDLIGDCPFSHRVLLTLEEKKLCYEVHLINLSDKPSWFLEVSPGGKV 60
           MA+E AVKAAVGAP++IGDCPFS RVLLTLEEKK+ Y++HLINLSDKP WFLEVSP GKV
Sbjct: 1   MAIEVAVKAAVGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVSPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120
           PVVKFDDKWVSDSDVIVGILEEKYPEPSLV+PPEFSSVGSK NGAFVKMLKSKDPNDGSE
Sbjct: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVTPPEFSSVGSKTNGAFVKMLKSKDPNDGSE 120

Query: 121 LALLEELKALDEHLKAHVCVLSISNNYIKAFMILLLIFAYAMRFQGPYVAGEKVTAVDLS 180
           LALLEELKALDEHLKAH                            GPYVAGEKVTAVDLS
Sbjct: 121 LALLEELKALDEHLKAH----------------------------GPYVAGEKVTAVDLS 180

Query: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKVAPEHVISGWEPKVNA 240
           LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTK APEHVISGWEPKVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 240

Query: 241 AAEPVISDSEPKVNAAAEPIISDSEPKANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGD 300
           AAEPVI                              SDSEPKAIEVAVKAAVGALDVIGD
Sbjct: 241 AAEPVI------------------------------SDSEPKAIEVAVKAAVGALDVIGD 300

Query: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360
           CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI
Sbjct: 301 CPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGI 360

Query: 361 LEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAHGP 420
           LEEKYP+P LATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHL AHGP
Sbjct: 361 LEEKYPEPFLATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLKAHGP 420

Query: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 480
           YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP
Sbjct: 421 YVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAP 436

Query: 481 EHVISGWEAKVNAA 495
           EHVISGWEAKVNAA
Sbjct: 481 EHVISGWEAKVNAA 436

BLAST of Carg23785 vs. TAIR 10
Match: AT1G75270.1 (dehydroascorbate reductase 2 )

HSP 1 Score: 320.9 bits (821), Expect = 1.8e-87
Identity = 153/212 (72.17%), Postives = 182/212 (85.85%), Query Frame = 0

Query: 283 AIEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVP 342
           A+++ VK AVGA DV+GDCPF+QRVLLTLEEKK+PYK HLI++SDKP WFL++SPEGKVP
Sbjct: 2   ALDICVKVAVGAPDVLGDCPFSQRVLLTLEEKKLPYKTHLINVSDKPQWFLDISPEGKVP 61

Query: 343 VAKIDDKWVPDSDVIVGILEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQ 402
           V K+D KWV DSDVIVG+LEEKYP+P L TPPEF+SVGSKI GAFV FLKSKD NDGSE+
Sbjct: 62  VVKLDGKWVADSDVIVGLLEEKYPEPSLKTPPEFASVGSKIFGAFVTFLKSKDANDGSEK 121

Query: 403 ALLEELKALNEHLNAH-GPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLS 462
           AL++EL+AL  HL  H GP+VAGEK+TAVDLSLAPKLYHL+VALGH+K W  PE+LTS+ 
Sbjct: 122 ALVDELEALENHLKTHSGPFVAGEKITAVDLSLAPKLYHLEVALGHYKNWSVPESLTSVR 181

Query: 463 KYKELLFARESFVKTKAAPEHVISGWEAKVNA 494
            Y + LF+RESF  TKA  E V++GWE+KVNA
Sbjct: 182 NYAKALFSRESFENTKAKKEIVVAGWESKVNA 213

BLAST of Carg23785 vs. TAIR 10
Match: AT1G19570.1 (dehydroascorbate reductase )

HSP 1 Score: 293.1 bits (749), Expect = 4.1e-79
Identity = 144/211 (68.25%), Postives = 167/211 (79.15%), Query Frame = 0

Query: 283 AIEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVP 342
           A+E+ VKAAVGA D +GDCPF+QR LLTLEEK + YK HLI+LSDKP WFL++SP+GKVP
Sbjct: 2   ALEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQWFLDISPQGKVP 61

Query: 343 VAKIDDKWVPDSDVIVGILEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQ 402
           V KIDDKWV DSDVIVGILEEKYP P L TP EF+SVGS I G F  FLKSKD NDGSE 
Sbjct: 62  VLKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSEH 121

Query: 403 ALLEELKALNEHLNAH-GPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLS 462
           ALL EL+AL  HL +H GP++AGE+V+AVDLSLAPKLYHL VALGHFK W  PE+   + 
Sbjct: 122 ALLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHVH 181

Query: 463 KYKELLFARESFVKTKAAPEHVISGWEAKVN 493
            Y + LF+ +SF KTK   ++VISGW  KVN
Sbjct: 182 NYMKTLFSLDSFEKTKTEEKYVISGWAPKVN 212

BLAST of Carg23785 vs. TAIR 10
Match: AT1G19570.2 (dehydroascorbate reductase )

HSP 1 Score: 284.3 bits (726), Expect = 1.9e-76
Identity = 143/211 (67.77%), Postives = 166/211 (78.67%), Query Frame = 0

Query: 283 AIEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVP 342
           A+E+ VKAAVGA D +GDCPF+QR LLTLEEK + YK HLI+LSDKP  FL++SP+GKVP
Sbjct: 2   ALEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQ-FLDISPQGKVP 61

Query: 343 VAKIDDKWVPDSDVIVGILEEKYPKPILATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQ 402
           V KIDDKWV DSDVIVGILEEKYP P L TP EF+SVGS I G F  FLKSKD NDGSE 
Sbjct: 62  VLKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSEH 121

Query: 403 ALLEELKALNEHLNAH-GPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLS 462
           ALL EL+AL  HL +H GP++AGE+V+AVDLSLAPKLYHL VALGHFK W  PE+   + 
Sbjct: 122 ALLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHVH 181

Query: 463 KYKELLFARESFVKTKAAPEHVISGWEAKVN 493
            Y + LF+ +SF KTK   ++VISGW  KVN
Sbjct: 182 NYMKTLFSLDSFEKTKTEEKYVISGWAPKVN 211

BLAST of Carg23785 vs. TAIR 10
Match: AT5G16710.1 (dehydroascorbate reductase 1 )

HSP 1 Score: 270.4 bits (690), Expect = 2.9e-72
Identity = 126/225 (56.00%), Postives = 163/225 (72.44%), Query Frame = 0

Query: 267 KANAVAEHVVSDSEPKAIEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLS 326
           K   V   V   +    +E+ VKA++   + +GDCPF Q+VLLT+EEK VPY   ++DLS
Sbjct: 32  KPGRVGRFVTMATAASPLEICVKASITTPNKLGDCPFCQKVLLTMEEKNVPYDMKMVDLS 91

Query: 327 DKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGILEEKYPKPILATPPEFSSVGSKISGA 386
           +KP WFL++SPEGKVPV K D+KWVPDSDVI   LEEKYP+P LATPPE +SVGSKI   
Sbjct: 92  NKPEWFLKISPEGKVPVVKFDEKWVPDSDVITQALEEKYPEPPLATPPEKASVGSKIFST 151

Query: 387 FVKFLKSKDPNDGSEQALLEELKALNEHLNAHGPYVAGEKVTAVDLSLAPKLYHLDVALG 446
           FV FLKSKD  DG+EQ LL+EL   N+++  +GP++ GEK++A DLSLAPKLYH+ +ALG
Sbjct: 152 FVGFLKSKDSGDGTEQVLLDELTTFNDYIKDNGPFINGEKISAADLSLAPKLYHMKIALG 211

Query: 447 HFKKWIFPENLTSLSKYKELLFARESFVKTKAAPEHVISGWEAKV 492
           H+K W  P++L  +  Y E +F+RESF  T+A  E VI+GW  KV
Sbjct: 212 HYKNWSVPDSLPFVKSYMENVFSRESFTNTRAETEDVIAGWRPKV 256

BLAST of Carg23785 vs. TAIR 10
Match: AT1G19550.1 (Glutathione S-transferase family protein )

HSP 1 Score: 170.2 bits (430), Expect = 4.0e-42
Identity = 90/174 (51.72%), Postives = 109/174 (62.64%), Query Frame = 0

Query: 322 LIDLSDKPS--WFLEVSPEGKVPVAKIDDKWVPDSDVIVGILEEKYPKPILATPPEFSSV 381
           ++ +   PS   F ++SP+GKVPV KIDDKWV DSD  VGILEEKYP P L TP EF+SV
Sbjct: 3   VVQICSSPSHEMFWDISPQGKVPVLKIDDKWVTDSDATVGILEEKYPDPPLKTPAEFASV 62

Query: 382 GSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLNAH-GPYVAGEKVTAVDLSLAPKL 441
           GS I                         +AL  HL +H GP++AGE+V+AVDLSLAPKL
Sbjct: 63  GSNI------------------------FEALENHLKSHDGPFIAGERVSAVDLSLAPKL 122

Query: 442 YHLDVALGHFKKWIFPENLTSLSKYKELLFARESFVKTKAAPEHVISGWEAKVN 493
           YHL VALGHFK W  PE+   +  Y + LF+ +SF KTK   + VISGW  KVN
Sbjct: 123 YHLQVALGHFKSWSVPESFPHVHNYMKTLFSLDSFEKTKTEEKCVISGWAPKVN 152

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030743.11.8e-281100.00Glutathione S-transferase DHAR2, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
KAG6600074.16.3e-25591.90Glutathione S-transferase DHAR2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022943189.16.5e-25290.69uncharacterized protein LOC111447994 isoform X1 [Cucurbita moschata][more]
XP_023547263.11.7e-24789.07uncharacterized protein LOC111806131 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022985936.16.3e-24788.46uncharacterized protein LOC111483829 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FRL82.6e-8672.17Glutathione S-transferase DHAR2 OS=Arabidopsis thaliana OX=3702 GN=DHAR2 PE=1 SV... [more]
Q65XA04.8e-8067.77Probable glutathione S-transferase DHAR1, cytosolic OS=Oryza sativa subsp. japon... [more]
Q9FWR45.8e-7868.25Glutathione S-transferase DHAR1, mitochondrial OS=Arabidopsis thaliana OX=3702 G... [more]
Q9FG591.5e-7364.95Probable glutathione S-transferase DHAR4 OS=Arabidopsis thaliana OX=3702 GN=DHAR... [more]
Q8LE524.0e-7156.00Glutathione S-transferase DHAR3, chloroplastic OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A6J1FTJ73.1e-25290.69uncharacterized protein LOC111447994 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1J9M23.0e-24788.46uncharacterized protein LOC111483829 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FXI24.3e-24187.65probable glutathione S-transferase DHAR2, chloroplastic isoform X2 OS=Cucurbita ... [more]
A0A6J1JEQ32.9e-23786.23probable glutathione S-transferase DHAR2, chloroplastic isoform X2 OS=Cucurbita ... [more]
A0A6J1FR131.1e-23184.82uncharacterized protein LOC111447994 isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT1G75270.11.8e-8772.17dehydroascorbate reductase 2 [more]
AT1G19570.14.1e-7968.25dehydroascorbate reductase [more]
AT1G19570.21.9e-7667.77dehydroascorbate reductase [more]
AT5G16710.12.9e-7256.00dehydroascorbate reductase 1 [more]
AT1G19550.14.0e-4251.72Glutathione S-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004045Glutathione S-transferase, N-terminalPFAMPF13417GST_N_3coord: 19..87
e-value: 3.1E-15
score: 56.3
coord: 300..368
e-value: 1.5E-16
score: 60.5
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 291..369
score: 15.398541
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 10..88
score: 14.715783
NoneNo IPR availableGENE3D1.20.1050.10coord: 371..493
e-value: 6.7E-46
score: 157.3
NoneNo IPR availablePFAMPF13410GST_C_2coord: 400..452
e-value: 6.1E-6
score: 26.2
NoneNo IPR availableGENE3D1.20.1050.10coord: 90..245
e-value: 1.3E-37
score: 130.4
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 282..370
e-value: 8.9E-28
score: 98.2
coord: 1..89
e-value: 1.7E-27
score: 97.3
NoneNo IPR availableSFLDSFLDG00358Main_(cytGST)coord: 300..462
e-value: 1.7E-26
score: 87.8
NoneNo IPR availablePANTHERPTHR44420:SF2GLUTATHIONE S-TRANSFERASE DHAR2-RELATEDcoord: 162..239
NoneNo IPR availablePANTHERPTHR44420:SF2GLUTATHIONE S-TRANSFERASE DHAR2-RELATEDcoord: 1..138
coord: 284..492
NoneNo IPR availableCDDcd00570GST_N_familycoord: 301..358
e-value: 1.1237E-14
score: 66.8264
NoneNo IPR availableCDDcd00570GST_N_familycoord: 20..77
e-value: 5.25479E-14
score: 64.9004
IPR040079Glutathione Transferase familySFLDSFLDS00019Glutathione_Transferase_(cytocoord: 300..462
e-value: 1.7E-26
score: 87.8
coord: 20..196
e-value: 1.0E-20
score: 69.0
IPR044627Dehydroascorbate reductases DHAR1/2/3/4PANTHERPTHR44420GLUTATHIONE S-TRANSFERASE DHAR2-RELATEDcoord: 162..239
coord: 284..492
IPR044627Dehydroascorbate reductases DHAR1/2/3/4PANTHERPTHR44420GLUTATHIONE S-TRANSFERASE DHAR2-RELATEDcoord: 1..138
IPR010987Glutathione S-transferase, C-terminal-likePROSITEPS50405GST_CTERcoord: 347..493
score: 10.276933
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 298..367
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 19..87
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 385..490
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 100..237

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23785-RACarg23785-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0033355 ascorbate glutathione cycle
biological_process GO:0098869 cellular oxidant detoxification
biological_process GO:0006749 glutathione metabolic process
molecular_function GO:0045174 glutathione dehydrogenase (ascorbate) activity
molecular_function GO:0004364 glutathione transferase activity
molecular_function GO:0005515 protein binding