Carg23613 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23613
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	alpha/beta-Hydrolases superfamily protein
Location	Carg_Chr20: 4538328 .. 4540237 (+)
RNA-Seq Expression	Carg23613
Synteny	Carg23613

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCTTCAACAAACGACGTCGCCGACGAGTTCTTTCCCTTCTTAAGAATCTACAAAGACGGCCGCATCCAACGACTCCAAGTCGCCTCCGACATAGTCTCCGCCTGCGCCGACGACCCCAACTCCGCTTTCCGAGCAAAGGATGTCACGATCTCCGTGGATTCCGCCCTTTCGGTGCGTATTTTCATCCCCTCCGCCGTAGATCTGAATCAGAAGCTGCCCCTTCTGTTGTACGTCCACGGCGGTGCCTTCTGCTTCGAATCGGCTTTTAATTTGCAGTACCATAGCCACGTGGGTTCTCTCGCTGCTAAGGCCAACGCCGTCGCTGTTTCCGTCGAGTACCGCCTCGCGCCGGAGAATCCAATCCCCGCCTGCTACGACGATTGTTGGGATGCGTTACGGTGGATCGCGGCGCATGTGAACAGGTGAGGCTGATTTCAATTTTGAATTTATTTATTTATTTATTTATTTTTGCTTGGGTAAAGTTTTTATCTCTACGTTACTAGTTTTTTAATATATATTTTTTTTAACTTATTAAAATAATACATACGATTTCATTATTAATATATATTTTAATAATAAAAATAAAAATATTTAGATATTTTAATTTTTTTTTATAAATACACAATCGCACTTTTGATGGCAGTAGACTTGCGATTGTGCGGCACTCACTTTCCAACGACAAGTGAGCTAAGCCAATTTGTCCTTGCGTCGCTTATAGCCAAGCGACGTATGCTCGAGTCTCTGGCCTCGATTTAAGAAGGTTTTAGAAAACAAGTGCAGAGATAAATTTTGTTAAGAGACTTTATATATAAAGTAAGAGAGTAACAATAACCGCTCACAATATATAACTTTTGATAGATGGATAGAAATTAAAATAAGACTTAGTTATTTTAGGAATAAAACGTTTAGATATTTTTAAGAATAAATTTGGTCCTTAGTTTCCCGCTATAAATGCCATTTCACCCGATTTTTAGTTTGTTTAACATCGAGTATCATTATCGGGACGGAGAATATATTCCTCGTCCGAGGCGAGGAACCCCGTCTAAATTCTTAAAAATTGAGTAAATCTATCTCTGGAACGATTTTATTTTAAAAATCATTCTCGTTAAAATATCATCACTAGCCTAATAGCGTCAAGTCTAATAATTGTCTAATCGTGTGATTACCCTTCAAAGTTTTCATACAAGAAATTACATGAAAATGTTTGCTTAATTCACCGCACCTAAATGATGTGAAAGTTATCTTTATTTATAATCAAATAAACTATCATATCTGATATGGAAAGAGCAATAAATGGAAAGAATAATAGATGAAAGATAGTTAGAATAAAAAAAAAAAGTAAATATCTAGATATTTGTCTACAATAAAATATCAAATATAATATATATATATATATTAATTTAGTACACAAAACCTTGGGCGCCAACAGGGATGGTCCAGAGCCGTGGCTGAACCAGCACGCAGATTTGGACCGGATCTGTTTAGCTGGCGACAGTGCGGGCGCCAACATCTGCCATCACTTGGCTGTTCGTGCAGGAACATCAGGAGAGCAGCTGGGCAGAGTAAAGGTCGCGGCATTGGCTTTGATTCACCCGTTCTTTGGCGACGGGGCGGGGGACCGGCTGTGGAAGTATCTGTGTTCGGACACCACTTGGCTGAGGCCCACCGCACAGGATCTCGCCACGTTGCCTTGTCGGAGAGTGAAGATCTTTCTGGCAGAGAAAGATTCTCTGAAGTTCGGAGGGCGGAATTACGATGAGGATCTGAAGAGCAGCGGGTGGAAGGGGGCGGTGGAGACGGTGGAGCACGGCGGAGAGGATCATGTGTTTCATTTGAAGATGCCGGAGTGTGAAAAGGCCGTTGATTTGATGGAAAAATTATCCTGTTTCATAAACTTAGATTAG

mRNA sequence

ATGGCCTCTTCAACAAACGACGTCGCCGACGAGTTCTTTCCCTTCTTAAGAATCTACAAAGACGGCCGCATCCAACGACTCCAAGTCGCCTCCGACATAGTCTCCGCCTGCGCCGACGACCCCAACTCCGCTTTCCGAGCAAAGGATGTCACGATCTCCGTGGATTCCGCCCTTTCGGTGCGTATTTTCATCCCCTCCGCCGTAGATCTGAATCAGAAGCTGCCCCTTCTGTTGTACGTCCACGGCGGTGCCTTCTGCTTCGAATCGGCTTTTAATTTGCAGTACCATAGCCACGTGGGTTCTCTCGCTGCTAAGGCCAACGCCGTCGCTGTTTCCGTCGAGTACCGCCTCGCGCCGGAGAATCCAATCCCCGCCTGCTACGACGATTGTTGGGATGCGTTACGGTGGATCGCGGCGCATGTGAACAGGGATGGTCCAGAGCCGTGGCTGAACCAGCACGCAGATTTGGACCGGATCTGTTTAGCTGGCGACAGTGCGGGCGCCAACATCTGCCATCACTTGGCTGTTCGTGCAGGAACATCAGGAGAGCAGCTGGGCAGAGTAAAGGTCGCGGCATTGGCTTTGATTCACCCGTTCTTTGGCGACGGGGCGGGGGACCGGCTGTGGAAGTATCTGTGTTCGGACACCACTTGGCTGAGGCCCACCGCACAGGATCTCGCCACGTTGCCTTGTCGGAGAGTGAAGATCTTTCTGGCAGAGAAAGATTCTCTGAAGTTCGGAGGGCGGAATTACGATGAGGATCTGAAGAGCAGCGGGTGGAAGGGGGCGGTGGAGACGGTGGAGCACGGCGGAGAGGATCATGTGTTTCATTTGAAGATGCCGGAGTGTGAAAAGGCCGTTGATTTGATGGAAAAATTATCCTGTTTCATAAACTTAGATTAG

Coding sequence (CDS)

ATGGCCTCTTCAACAAACGACGTCGCCGACGAGTTCTTTCCCTTCTTAAGAATCTACAAAGACGGCCGCATCCAACGACTCCAAGTCGCCTCCGACATAGTCTCCGCCTGCGCCGACGACCCCAACTCCGCTTTCCGAGCAAAGGATGTCACGATCTCCGTGGATTCCGCCCTTTCGGTGCGTATTTTCATCCCCTCCGCCGTAGATCTGAATCAGAAGCTGCCCCTTCTGTTGTACGTCCACGGCGGTGCCTTCTGCTTCGAATCGGCTTTTAATTTGCAGTACCATAGCCACGTGGGTTCTCTCGCTGCTAAGGCCAACGCCGTCGCTGTTTCCGTCGAGTACCGCCTCGCGCCGGAGAATCCAATCCCCGCCTGCTACGACGATTGTTGGGATGCGTTACGGTGGATCGCGGCGCATGTGAACAGGGATGGTCCAGAGCCGTGGCTGAACCAGCACGCAGATTTGGACCGGATCTGTTTAGCTGGCGACAGTGCGGGCGCCAACATCTGCCATCACTTGGCTGTTCGTGCAGGAACATCAGGAGAGCAGCTGGGCAGAGTAAAGGTCGCGGCATTGGCTTTGATTCACCCGTTCTTTGGCGACGGGGCGGGGGACCGGCTGTGGAAGTATCTGTGTTCGGACACCACTTGGCTGAGGCCCACCGCACAGGATCTCGCCACGTTGCCTTGTCGGAGAGTGAAGATCTTTCTGGCAGAGAAAGATTCTCTGAAGTTCGGAGGGCGGAATTACGATGAGGATCTGAAGAGCAGCGGGTGGAAGGGGGCGGTGGAGACGGTGGAGCACGGCGGAGAGGATCATGTGTTTCATTTGAAGATGCCGGAGTGTGAAAAGGCCGTTGATTTGATGGAAAAATTATCCTGTTTCATAAACTTAGATTAG

Protein sequence

MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSVRIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGTSGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD

Homology

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BLAST of Carg23613 vs. NCBI nr
Match: KAG7010967.1 (putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 625.5 bits (1612), Expect = 2.3e-175
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180

Query: 181 SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300
           KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300

[Close]

BLAST of Carg23613 vs. NCBI nr
Match: KAG6571158.1 (putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 622.1 bits (1603), Expect = 2.5e-174
Identity = 298/300 (99.33%), Postives = 299/300 (99.67%), Query Frame = 0


Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYKDGRIQRLQV SDIVSACADDP+SAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVSACADDPSSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180

Query: 181 SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300
           KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300

[Close]

BLAST of Carg23613 vs. NCBI nr
Match: XP_022944136.1 (probable carboxylesterase 12 [Cucurbita moschata])

HSP 1 Score: 618.2 bits (1593), Expect = 3.7e-173
Identity = 296/300 (98.67%), Postives = 296/300 (98.67%), Query Frame = 0


Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYKDGRIQRLQV SDIVSACADDPNSAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVSACADDPNSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYH HVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHRHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180

Query: 181 SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           S EQLGRVKV ALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300
           KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300

[Close]

BLAST of Carg23613 vs. NCBI nr
Match: XP_022986577.1 (probable carboxylesterase 12 [Cucurbita maxima] >XP_022986578.1 probable carboxylesterase 12 [Cucurbita maxima])

HSP 1 Score: 609.4 bits (1570), Expect = 1.7e-170
Identity = 291/300 (97.00%), Postives = 295/300 (98.33%), Query Frame = 0


Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYK+GRIQRLQV SDIV ACADDPNSAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKNGRIQRLQVTSDIVPACADDPNSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLN+HADLDRICLAGDSAGANICHHLAVRAG+
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNEHADLDRICLAGDSAGANICHHLAVRAGS 180

Query: 181 SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           S EQLGRVKV ALALIHPFFGDGA DRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SAEQLGRVKVVALALIHPFFGDGAEDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300
           KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKL+CFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300

[Close]

BLAST of Carg23613 vs. NCBI nr
Match: XP_023512082.1 (probable carboxylesterase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 608.2 bits (1567), Expect = 3.8e-170
Identity = 291/300 (97.00%), Postives = 293/300 (97.67%), Query Frame = 0


Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYKDGRIQRLQV SDIV ACADDP SAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAG+
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 180

Query: 181 SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           S EQLGRVKV ALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300
           KDSLKFGG NYDEDLKSSGW GAVETVEHGGEDHVFHLKMPECEKAVDLMEKL+CFINLD
Sbjct: 241 KDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300

[Close]

BLAST of Carg23613 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 4.2e-63
Identity = 131/327 (40.06%), Postives = 186/327 (56.88%), Query Frame = 0


Query: 6   NDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSVRIFIP 65
           +++A +  P L+IYK GRI+RL   + +    + +P +   +KDV  S D+ LSVRI++P
Sbjct: 3   SEIAVDCSPLLKIYKSGRIERLMGEATVPP--SSEPQNGVVSKDVVYSADNNLSVRIYLP 62

Query: 66  --SAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENPI 125
             +A + + KLPLL+Y HGG F  E+AF+  YH+ + +  + +N VAVSV+YR APE+PI
Sbjct: 63  EKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPI 122

Query: 126 PACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGTS-- 185
              +DD W AL+W+  H+   G E WLN+HAD  R+ L+GDSAGANI HH+A+RA     
Sbjct: 123 SVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKL 182

Query: 186 GEQLGRVKVAALALIHPFF-------------------------------GDGAGDRLWK 245
              L    ++ + L+HP+F                                DG  D L  
Sbjct: 183 SPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLN 242

Query: 246 YLCSDTTWLRPTAQDLATLPCRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHG 298
            + S++        DL+ L C +V + +AEKD+L   G  Y   L+ SGWKG VE VE  
Sbjct: 243 VVQSESV-------DLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESE 302

[Close]

BLAST of Carg23613 vs. ExPASy Swiss-Prot
Match: Q9FX94 (Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.0e-61
Identity = 126/319 (39.50%), Postives = 188/319 (58.93%), Query Frame = 0


Query: 6   NDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSVRIFIP 65
           +++A EF PF RIYKDGR++RL + +D + A   DP     +KDV  S ++ LSVR+F+P
Sbjct: 3   SEIASEFLPFCRIYKDGRVERL-IGTDTIPASL-DPTYDVVSKDVIYSPENNLSVRLFLP 62

Query: 66  ---SAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENP 125
              + +    KLPLL+Y+HGGA+  ES F+  YH+++  +   AN +AVSV+YR APE+P
Sbjct: 63  HKSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDP 122

Query: 126 IPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGTSG 185
           +PA Y+D W A++WI AH N  GP  W+N+HAD  ++ L GDSAG NI HH+A++AG   
Sbjct: 123 VPAAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGK-- 182

Query: 186 EQLGRVKVAALALIHP-FFGDGAGD--------------RLWKYLCSDTTW--------- 245
           E+   +K+  +A++HP F+G    D               +W+ + S  +          
Sbjct: 183 EKKLDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFN 242

Query: 246 LRPTAQDLATLPCRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHL 298
           +  +  D + L C +V + +A KD     G  Y   L+   W+G VE VE  GEDHVFHL
Sbjct: 243 VNGSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHL 302

[Close]

BLAST of Carg23613 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 7.4e-60
Identity = 133/320 (41.56%), Postives = 184/320 (57.50%), Query Frame = 0


Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV 60
           M S+   V+ E  P+L ++ DG ++RL  A   V     DP +   +KD+ I   + LS 
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERL--AGTEVCPPGLDPITGVFSKDIIIEPKTGLSA 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RI+ P ++   QK+PL+LY HGGAF   S     YH+ +  +  +AN +AVSV YRLAPE
Sbjct: 61  RIYRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180
           +P+P  Y+D W AL+ I A +N    EPW+N +ADLD + L GDSAGANI HHLA RA  
Sbjct: 121 HPLPTAYEDSWTALKNIQA-IN----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQ 180

Query: 181 SGEQLGRVKVAALALIHPFFGD----GAG----------DRLWKYLC-----SDTTWLRP 240
           S + L   K+  + +IHP+F      GA           D  W+++C     SD  W+ P
Sbjct: 181 SDQTL---KIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINP 240

Query: 241 TAQ---DLATLPCRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHL 299
            A    DL  L C RV I +AEKD L   G+ Y E L  S WKG VE +E   +DHVFH+
Sbjct: 241 FADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHI 300

[Close]

BLAST of Carg23613 vs. ExPASy Swiss-Prot
Match: Q9LMA7 (Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.8e-59
Identity = 120/313 (38.34%), Postives = 180/313 (57.51%), Query Frame = 0


Query: 6   NDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSVRIFIP 65
           +++A ++ P  RI+K+G I+RL   + +  +   +P +   +KD   S +  LS+RI++P
Sbjct: 3   SEIAFDYSPRFRIFKNGGIERLVPETFVPPSL--NPENGVVSKDAVYSPEKNLSLRIYLP 62

Query: 66  --SAVDLNQ-KLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENP 125
             S  +  + K+PLL+Y HGG F  E+AF+  YH+ + S  +  + +AVSVEYR APE+P
Sbjct: 63  QNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHP 122

Query: 126 IPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGTSG 185
           IP  Y+D WDA++WI  H+ R GPE WLN+HAD  ++ LAGDSAGANI HH+A+R     
Sbjct: 123 IPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEK 182

Query: 186 EQLGRVKVAALALIHPFFGDGA------------GDRLWKYLCSDT------TWLRPTAQ 245
                 K++ + L HP+F   A             +RLW+    D+       W+     
Sbjct: 183 LPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVGS 242

Query: 246 DLATLPCRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECE 298
           DL  L CRRV + +A  D L  GG +Y  +L+ SGW G V+ +E   E HVFHL+ P+ E
Sbjct: 243 DLTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSE 302

[Close]

BLAST of Carg23613 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.8e-59
Identity = 121/329 (36.78%), Postives = 189/329 (57.45%), Query Frame = 0


Query: 1   MASSTN-----DVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVD 60
           MASST+     ++  E  P LR+YKDG ++R  + S  V    +DP +    KD+ IS +
Sbjct: 1   MASSTSTTTSKEIDRELPPLLRVYKDGTVERF-LGSSFVPPSPEDPETGVSTKDIVISEN 60

Query: 61  SALSVRIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEY 120
             +S R+++P   +  +KLP+L+Y HGGAFC ESAF+  +  ++  +A+KAN + VS+EY
Sbjct: 61  PTISARVYLPKLNNTTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEY 120

Query: 121 RLAPENPIPACYDDCWDALRWIAAH-VNRDGP---EPWLNQHADLDRICLAGDSAGANIC 180
           RLAPE+P+PA Y+D W AL+W+ +H  N + P   +PWL +H D +R  + GD++GANI 
Sbjct: 121 RLAPEHPLPAAYEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIA 180

Query: 181 HHLAVRAGTSGEQLGRVKVAALALIHPFF--------------GDGAGDRLWKYLCSDTT 240
           H+ A+R G      G +++A +    P F                 +  ++W ++  D  
Sbjct: 181 HNAALRVGAEALP-GGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQVWNFVYPDAP 240

Query: 241 W---------LRPTAQDLATLPCRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVE 298
                     L P A +LATL C ++ +F+A KD L+  G  Y E +K SGWKG VE  +
Sbjct: 241 GGIDNPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQ 300

[Close]

BLAST of Carg23613 vs. ExPASy TrEMBL
Match: A0A6J1FTL3 (probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448682 PE=4 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.8e-173
Identity = 296/300 (98.67%), Postives = 296/300 (98.67%), Query Frame = 0


Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYKDGRIQRLQV SDIVSACADDPNSAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVSACADDPNSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYH HVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHRHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180

Query: 181 SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           S EQLGRVKV ALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300
           KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300

[Close]

BLAST of Carg23613 vs. ExPASy TrEMBL
Match: A0A6J1J7Y5 (probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484278 PE=4 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 8.2e-171
Identity = 291/300 (97.00%), Postives = 295/300 (98.33%), Query Frame = 0


Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYK+GRIQRLQV SDIV ACADDPNSAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKNGRIQRLQVTSDIVPACADDPNSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLN+HADLDRICLAGDSAGANICHHLAVRAG+
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNEHADLDRICLAGDSAGANICHHLAVRAGS 180

Query: 181 SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           S EQLGRVKV ALALIHPFFGDGA DRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SAEQLGRVKVVALALIHPFFGDGAEDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300
           KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKL+CFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300

[Close]

BLAST of Carg23613 vs. ExPASy TrEMBL
Match: A0A0A0LNZ1 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G234530 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.3e-135
Identity = 233/303 (76.90%), Postives = 259/303 (85.48%), Query Frame = 0


Query: 1   MASSTN---DVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSA 60
           MASSTN   DVA +FFPFLR+Y DGR+QRL   SDIV A ADDP S FR+KDVTIS D A
Sbjct: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  LSVRIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRL 120
           +S R+FIPS+ D NQKLPLLLYVHGGAFC ESAF+LQYH HVGSLAAKANAVAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL 120

Query: 121 APENPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVR 180
           APE+PIPACY+DCWDALRW+AAHVNRDG EPWLN + D +RICLAGDSAGANICH+LA R
Sbjct: 121 APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 AGTSGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIF 240
           A +S E+LG  KV A+ALIHPFFGDG  +RLWKYLCS+T  LRPT +DLA L C+RVKIF
Sbjct: 181 ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCSETKLLRPTIEDLAKLGCKRVKIF 240

Query: 241 LAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFI 300
           LAE D LK GG+NY+EDLKSSGW G VETVEHG E+HVFHLK PECEKAVDL+EKL+ FI
Sbjct: 241 LAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFI 300

[Close]

BLAST of Carg23613 vs. ExPASy TrEMBL
Match: A0A5D3CP06 (2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00380 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.0e-133
Identity = 229/303 (75.58%), Postives = 257/303 (84.82%), Query Frame = 0


Query: 1   MASST---NDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSA 60
           MASST   N+VA + FPFLR+Y DGR+QRL + SD V A ADDP S FR+KDVTIS D A
Sbjct: 1   MASSTNKNNEVAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  LSVRIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRL 120
           +S R+FIPS+ D NQKLPLLLYVHGGAFC ESAFNLQYH HVG+LAA+ANAVAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVEYRL 120

Query: 121 APENPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVR 180
           APE+PIPACYDDCWDALRW+AAHVNRDG EPWLN + D +RICLAGDSAGANICH+LA R
Sbjct: 121 APEHPIPACYDDCWDALRWVAAHVNRDGLEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 AGTSGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIF 240
           A +S E+LG  KV A+ALIHPFFGDG  DRLWKYLCS+T  +RPT ++LA + C+RVKIF
Sbjct: 181 ASSSSEELGGAKVVAMALIHPFFGDGREDRLWKYLCSETRLVRPTKEELAKMGCKRVKIF 240

Query: 241 LAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFI 300
           LAE D LKFGGRNYDEDLK SGW G VETVEHGGE+HVFHLK PE E AVDL+EKL+ FI
Sbjct: 241 LAENDFLKFGGRNYDEDLKRSGWNGVVETVEHGGENHVFHLKNPESENAVDLLEKLASFI 300

[Close]

BLAST of Carg23613 vs. ExPASy TrEMBL
Match: A0A1S3C9R7 (2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo OX=3656 GN=LOC103498401 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.0e-133
Identity = 229/303 (75.58%), Postives = 257/303 (84.82%), Query Frame = 0


Query: 1   MASST---NDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSA 60
           MASST   N+VA + FPFLR+Y DGR+QRL + SD V A ADDP S FR+KDVTIS D A
Sbjct: 1   MASSTNKNNEVAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  LSVRIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRL 120
           +S R+FIPS+ D NQKLPLLLYVHGGAFC ESAFNLQYH HVG+LAA+ANAVAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVEYRL 120

Query: 121 APENPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVR 180
           APE+PIPACYDDCWDALRW+AAHVNRDG EPWLN + D +RICLAGDSAGANICH+LA R
Sbjct: 121 APEHPIPACYDDCWDALRWVAAHVNRDGLEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 AGTSGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIF 240
           A +S E+LG  KV A+ALIHPFFGDG  DRLWKYLCS+T  +RPT ++LA + C+RVKIF
Sbjct: 181 ASSSSEELGGAKVVAMALIHPFFGDGREDRLWKYLCSETRLVRPTKEELAKMGCKRVKIF 240

Query: 241 LAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFI 300
           LAE D LKFGGRNYDEDLK SGW G VETVEHGGE+HVFHLK PE E AVDL+EKL+ FI
Sbjct: 241 LAENDFLKFGGRNYDEDLKRSGWNGVVETVEHGGENHVFHLKNPESENAVDLLEKLASFI 300

[Close]

BLAST of Carg23613 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 243.0 bits (619), Expect = 3.0e-64
Identity = 131/327 (40.06%), Postives = 186/327 (56.88%), Query Frame = 0


Query: 6   NDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSVRIFIP 65
           +++A +  P L+IYK GRI+RL   + +    + +P +   +KDV  S D+ LSVRI++P
Sbjct: 3   SEIAVDCSPLLKIYKSGRIERLMGEATVPP--SSEPQNGVVSKDVVYSADNNLSVRIYLP 62

Query: 66  --SAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENPI 125
             +A + + KLPLL+Y HGG F  E+AF+  YH+ + +  + +N VAVSV+YR APE+PI
Sbjct: 63  EKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPI 122

Query: 126 PACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGTS-- 185
              +DD W AL+W+  H+   G E WLN+HAD  R+ L+GDSAGANI HH+A+RA     
Sbjct: 123 SVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKL 182

Query: 186 GEQLGRVKVAALALIHPFF-------------------------------GDGAGDRLWK 245
              L    ++ + L+HP+F                                DG  D L  
Sbjct: 183 SPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLN 242

Query: 246 YLCSDTTWLRPTAQDLATLPCRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHG 298
            + S++        DL+ L C +V + +AEKD+L   G  Y   L+ SGWKG VE VE  
Sbjct: 243 VVQSESV-------DLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESE 302

[Close]

BLAST of Carg23613 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 236.9 bits (603), Expect = 2.1e-62
Identity = 126/319 (39.50%), Postives = 188/319 (58.93%), Query Frame = 0


Query: 6   NDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSVRIFIP 65
           +++A EF PF RIYKDGR++RL + +D + A   DP     +KDV  S ++ LSVR+F+P
Sbjct: 3   SEIASEFLPFCRIYKDGRVERL-IGTDTIPASL-DPTYDVVSKDVIYSPENNLSVRLFLP 62

Query: 66  ---SAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENP 125
              + +    KLPLL+Y+HGGA+  ES F+  YH+++  +   AN +AVSV+YR APE+P
Sbjct: 63  HKSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDP 122

Query: 126 IPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGTSG 185
           +PA Y+D W A++WI AH N  GP  W+N+HAD  ++ L GDSAG NI HH+A++AG   
Sbjct: 123 VPAAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGK-- 182

Query: 186 EQLGRVKVAALALIHP-FFGDGAGD--------------RLWKYLCSDTTW--------- 245
           E+   +K+  +A++HP F+G    D               +W+ + S  +          
Sbjct: 183 EKKLDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFN 242

Query: 246 LRPTAQDLATLPCRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHL 298
           +  +  D + L C +V + +A KD     G  Y   L+   W+G VE VE  GEDHVFHL
Sbjct: 243 VNGSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHL 302

[Close]

BLAST of Carg23613 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 5.3e-61
Identity = 133/320 (41.56%), Postives = 184/320 (57.50%), Query Frame = 0


Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV 60
           M S+   V+ E  P+L ++ DG ++RL  A   V     DP +   +KD+ I   + LS 
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERL--AGTEVCPPGLDPITGVFSKDIIIEPKTGLSA 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RI+ P ++   QK+PL+LY HGGAF   S     YH+ +  +  +AN +AVSV YRLAPE
Sbjct: 61  RIYRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180
           +P+P  Y+D W AL+ I A +N    EPW+N +ADLD + L GDSAGANI HHLA RA  
Sbjct: 121 HPLPTAYEDSWTALKNIQA-IN----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQ 180

Query: 181 SGEQLGRVKVAALALIHPFFGD----GAG----------DRLWKYLC-----SDTTWLRP 240
           S + L   K+  + +IHP+F      GA           D  W+++C     SD  W+ P
Sbjct: 181 SDQTL---KIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINP 240

Query: 241 TAQ---DLATLPCRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHL 299
            A    DL  L C RV I +AEKD L   G+ Y E L  S WKG VE +E   +DHVFH+
Sbjct: 241 FADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHI 300

[Close]

BLAST of Carg23613 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 230.3 bits (586), Expect = 2.0e-60
Identity = 120/313 (38.34%), Postives = 180/313 (57.51%), Query Frame = 0


Query: 6   NDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSVRIFIP 65
           +++A ++ P  RI+K+G I+RL   + +  +   +P +   +KD   S +  LS+RI++P
Sbjct: 3   SEIAFDYSPRFRIFKNGGIERLVPETFVPPSL--NPENGVVSKDAVYSPEKNLSLRIYLP 62

Query: 66  --SAVDLNQ-KLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENP 125
             S  +  + K+PLL+Y HGG F  E+AF+  YH+ + S  +  + +AVSVEYR APE+P
Sbjct: 63  QNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHP 122

Query: 126 IPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGTSG 185
           IP  Y+D WDA++WI  H+ R GPE WLN+HAD  ++ LAGDSAGANI HH+A+R     
Sbjct: 123 IPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEK 182

Query: 186 EQLGRVKVAALALIHPFFGDGA------------GDRLWKYLCSDT------TWLRPTAQ 245
                 K++ + L HP+F   A             +RLW+    D+       W+     
Sbjct: 183 LPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVGS 242

Query: 246 DLATLPCRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECE 298
           DL  L CRRV + +A  D L  GG +Y  +L+ SGW G V+ +E   E HVFHL+ P+ E
Sbjct: 243 DLTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSE 302

[Close]

BLAST of Carg23613 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 223.4 bits (568), Expect = 2.4e-58
Identity = 122/312 (39.10%), Postives = 173/312 (55.45%), Query Frame = 0


Query: 8   VADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSVRIFIPSA 67
           +A +  P  R+YK GRI+RL   + +  +    P +   +KD+  S +  LS+RI++P  
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLT--PQNGVVSKDIIHSPEKNLSLRIYLPEK 64

Query: 68  VDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENPIPACY 127
           V + +KLP+L+Y HGG F  E+AF+  YH+ + S  A AN +A+SV YR APE P+P  Y
Sbjct: 65  VTV-KKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIPY 124

Query: 128 DDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGTSGEQLGR 187
           +D WD+L+W+  H+   GPE W+N+H D  ++ LAGDSAG NI HHL +RA    E+L  
Sbjct: 125 EDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRA--KKEKLCD 184

Query: 188 VKVAALALIHPFF--------------GDGAG-DRLWKYLCS------DTTWLRPTAQDL 247
             ++ + LIHP+F              G   G +  W+          D  WL     D 
Sbjct: 185 SLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDP 244

Query: 248 ATLPCRRVKIFLAEKDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKA 299
           + L C RV + +A  D     G  Y E LK SGW+G VE +E   E HVFHLK P  + A
Sbjct: 245 SGLGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNA 304

The following BLAST results are available for this feature:

BLAST of Carg23613 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010967.1	2.3e-175	100.00	putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
KAG6571158.1	2.5e-174	99.33	putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022944136.1	3.7e-173	98.67	probable carboxylesterase 12 [Cucurbita moschata]	[more]
XP_022986577.1	1.7e-170	97.00	probable carboxylesterase 12 [Cucurbita maxima] >XP_022986578.1 probable carboxy...	[more]
XP_023512082.1	3.8e-170	97.00	probable carboxylesterase 12 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg23613 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SMN0	4.2e-63	40.06	Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1	[more]
Q9FX94	3.0e-61	39.50	Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1	[more]
Q9SX78	7.4e-60	41.56	Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1	[more]
Q9LMA7	2.8e-59	38.34	Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1	[more]
Q5NUF4	2.8e-59	36.78	2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 ...	[more]

BLAST of Carg23613 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FTL3	1.8e-173	98.67	probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448682 PE=4 ...	[more]
A0A6J1J7Y5	8.2e-171	97.00	probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484278 PE=4 SV...	[more]
A0A0A0LNZ1	3.3e-135	76.90	Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G2345...	[more]
A0A5D3CP06	1.0e-133	75.58	2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C9R7	1.0e-133	75.58	2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo OX=3656 GN=LOC103498401 P...	[more]

BLAST of Carg23613 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48690.1	3.0e-64	40.06	alpha/beta-Hydrolases superfamily protein	[more]
AT1G49660.1	2.1e-62	39.50	carboxyesterase 5	[more]
AT1G47480.1	5.3e-61	41.56	alpha/beta-Hydrolases superfamily protein	[more]
AT1G19190.1	2.0e-60	38.34	alpha/beta-Hydrolases superfamily protein	[more]
AT2G03550.1	2.4e-58	39.10	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 77..199 e-value: 2.0E-35 score: 122.6
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 15..299 e-value: 2.1E-62 score: 213.5
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 17..298
None	No IPR available	PANTHER	PTHR23024	ARYLACETAMIDE DEACETYLASE	coord: 5..298
None	No IPR available	PANTHER	PTHR23024:SF514	CARBOXYLESTERASE 12-RELATED	coord: 5..298
IPR033140	Lipase, GDXG, putative serine active site	PROSITE	PS01174	LIPASE_GDXG_SER	coord: 159..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23613-RA	Carg23613-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity