Carg23546 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23546
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	transmembrane emp24 domain-containing protein p24beta2-like
Location	Carg_Chr06: 3459522 .. 3461692 (+)
RNA-Seq Expression	Carg23546
Synteny	Carg23546

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGATTGTGTTTATTTGTGGAGTTTAAGGAAAAAATGGAGATGGGAAGTGGGAAGTCAAAATACAGGTTTTTTATTTTCATGGCGGCTTTGTTCATATCATCACACCCAGTTTTGGGGATTAGATTTGTGATTGACAAAGAAGAATGCTTCTCTCACAACGTTGAATTCATTAGAGATACCATTTATCTCTCTTTTGTTGTCGTTAGCGTTCATACCTGGCGACATCATGCTCACAACGGCGTTGATCTTTTGGTGACCACTTTTATCAATTACTCTTTTTTCTTTTTCTCTCTTAGATTCGTCAAAATTGTTTGTATTAATTTGCATGTTTATGAGATGTCCGGCTCGTGAATTAGGATTAAGATATTGAATCTGATTGTATATACGTGTTGTTGTTATATACCTTACAGATGTTAAGGAACCGAGCCTTTTGTTTTGCACGTATGTATGGGTGAATGAGATTCAAATTAGGTTCTATGCATTAGGGTTTAAAAAACTAACATTGTTTGATATATGTTATTATAAGTTTTTTCAAAATGTTTGTATTCGATTTATAGATTAAGTTTTATAAAACTCCATTTTAGTAACATTTACAGATCATAGATCTGTTCATCACAGTGACACAAACCTAAATATAATACAAATCAAGCTTTTCCCAATCCGCAATTTTATAAATTCGAGCATCGTCTCAAGTCTCTCGATGTCGAAACTAAGTCATCAGTTCATGTTTTAGTCTTGTTATGATTAAATGTGATTTGCAAAGTACAGGTGAAAGGGCCTTTAGGGGAGAGAATTGAAGAAATTAGAGGGAAAACAAGTGAGAAATTTGAGTTTATTTCTCTTAGAAAAGGGCGATATCACTTCTGCTTCATCAACAACTCACCTCACCAAGAGACCATAGAATTTGATATCCATATCAATCATTACTATGAGTTCTATGATGATGATGAACAACATGTAAAAGATGGTATTAATTCCTTTGATGAACCCTAAAGTTTTTGCTTTGAAATTCATACTCCCCATTACTTTTCTGCTCATCTCATCTGGTTTGAAATGAAATTTACAGAACATTTGAGCCCTTTATTGGCTCAAATAAAGAAGTTGGGAGAAACCTTAGGCGTTATTGAGTTTGAGCAGCAATGGTTAGAGGCACAGACAGAGCGACAAGCCAAAGGTATTGTTCATCTATATTAACCCATTTGATATACAATCTATTCCTTTGTATTAATTCAAATATCAGATCTAAACAATAACACTTCTACCATTTAGTAAGTGAAGTACCTTTTTTGCAGTGAATGAAGCAATGAGCACAATGGCGATTCTGAAGGCTGTTCTCGAATCAGCAGCGCTCATCGGTGCAAGCAGTCTTCAAGTTTTCCTTCTACGTCGCCTGTTCGAGAAAAAAGTTGGAAGCCTGTATTCGAGCACCTACAGAATATAATCTCATATCCAAGGCAGATTCAAACTCCAGCAGACATACAACAAGCATGGCAGAACAAGTGTTGGAAAGGTTGAGGAGTTTTTTTTTGTTTCTCTGTTTTTAATGAAACTAAAAGAAGTCATCAAATTTGGTTTAGAGCTAAAACTATTCATCCTTATAAGATGATCGGCTTCCGAATGGTTGAATAATTACTTTCTCAACATCTAATACGCCATGATGAATCGGTGTAACGAGTATCCATGTTCGTATTATTCTTTCTTCTAATGTTTGCCAACATTTTCAACATAAATATTATGCTCCATGCTTAGAAAGCAAGAGAATATTATGCTCTCCATGAGGCATGGACTTTCTCTAAAGGGTTGTTTTCGAGTAATCGAAAATCTTCTACAGGGGAAAATGAAAATGGAAAGAGAGAAAGAAACCATTTAACAAACAATATTAGAGATTCAATATCAGATCTAAGCAGCAACATGCGTTCTTAAAAAAAACAACAAAAAAGAAGTAAAAACAAATTAGATAGCACAGGTCAGCTTCTACCAACACTTCACAGCACCACAAATAAAGCACGTAAAATTCTTCAACTTTTTTGCTCCATGAATGAAGACTAAGAAGCGAATAGTATAGCAGGCTGACCGCAATCGAAAACCAGTGGAGACGATCACAGAGCAACACCAGTGGAGACGATCACAGAGCAACACGGTTACGGGCTCTGAATGCAGCAGAACCAT

mRNA sequence

TTTGATTGTGTTTATTTGTGGAGTTTAAGGAAAAAATGGAGATGGGAAGTGGGAAGTCAAAATACAGGTTTTTTATTTTCATGGCGGCTTTGTTCATATCATCACACCCAGTTTTGGGGATTAGATTTGTGATTGACAAAGAAGAATGCTTCTCTCACAACGTTGAATTCATTAGAGATACCATTTATCTCTCTTTTGTTGTCGTTAGCGTTCATACCTGGCGACATCATGCTCACAACGGCGTTGATCTTTTGGTGAAAGGGCCTTTAGGGGAGAGAATTGAAGAAATTAGAGGGAAAACAAGTGAGAAATTTGAGTTTATTTCTCTTAGAAAAGGGCGATATCACTTCTGCTTCATCAACAACTCACCTCACCAAGAGACCATAGAATTTGATATCCATATCAATCATTACTATGAGTTCTATGATGATGATGAACAACATGTAAAAGATGAACATTTGAGCCCTTTATTGGCTCAAATAAAGAAGTTGGGAGAAACCTTAGGCGTTATTGAGTTTGAGCAGCAATGGTTAGAGGCACAGACAGAGCGACAAGCCAAAGTGAATGAAGCAATGAGCACAATGGCGATTCTGAAGGCTGTTCTCGAATCAGCAGCGCTCATCGGTGCAAGCAGTCTTCAAGTTTTCCTTCTACGTCGCCTGTTCGAGAAAAAAGTTGGAAGCCTGTATTCGAGCACCTACAGAATATAATCTCATATCCAAGGCAGATTCAAACTCCAGCAGACATACAACAAGCATGGCAGAACAAGTGTTGGAAAGGTTGAGGAGTTTTTTTTTGTTTCTCTGTTTTTAATGAAACTAAAAGAAGTCATCAAATTTGGTTTAGAGCTAAAACTATTCATCCTTATAAGATGATCGGCTTCCGAATGGTTGAATAATTACTTTCTCAACATCTAATACGCCATGATGAATCGGTGTAACGAGTATCCATGTTCGTATTATTCTTTCTTCTAATGTTTGCCAACATTTTCAACATAAATATTATGCTCCATGCTTAGAAAGCAAGAGAATATTATGCTCTCCATGAGGCATGGACTTTCTCTAAAGGGTTGTTTTCGAGTAATCGAAAATCTTCTACAGGGGAAAATGAAAATGGAAAGAGAGAAAGAAACCATTTAACAAACAATATTAGAGATTCAATATCAGATCTAAGCAGCAACATGCGTTCTTAAAAAAAACAACAAAAAAGAAGTAAAAACAAATTAGATAGCACAGGTCAGCTTCTACCAACACTTCACAGCACCACAAATAAAGCACGTAAAATTCTTCAACTTTTTTGCTCCATGAATGAAGACTAAGAAGCGAATAGTATAGCAGGCTGACCGCAATCGAAAACCAGTGGAGACGATCACAGAGCAACACGGTTACGGGCTCTGAATGCAGCAGAACCAT

Coding sequence (CDS)

ATGGAGATGGGAAGTGGGAAGTCAAAATACAGGTTTTTTATTTTCATGGCGGCTTTGTTCATATCATCACACCCAGTTTTGGGGATTAGATTTGTGATTGACAAAGAAGAATGCTTCTCTCACAACGTTGAATTCATTAGAGATACCATTTATCTCTCTTTTGTTGTCGTTAGCGTTCATACCTGGCGACATCATGCTCACAACGGCGTTGATCTTTTGGTGAAAGGGCCTTTAGGGGAGAGAATTGAAGAAATTAGAGGGAAAACAAGTGAGAAATTTGAGTTTATTTCTCTTAGAAAAGGGCGATATCACTTCTGCTTCATCAACAACTCACCTCACCAAGAGACCATAGAATTTGATATCCATATCAATCATTACTATGAGTTCTATGATGATGATGAACAACATGTAAAAGATGAACATTTGAGCCCTTTATTGGCTCAAATAAAGAAGTTGGGAGAAACCTTAGGCGTTATTGAGTTTGAGCAGCAATGGTTAGAGGCACAGACAGAGCGACAAGCCAAAGTGAATGAAGCAATGAGCACAATGGCGATTCTGAAGGCTGTTCTCGAATCAGCAGCGCTCATCGGTGCAAGCAGTCTTCAAGTTTTCCTTCTACGTCGCCTGTTCGAGAAAAAAGTTGGAAGCCTGTATTCGAGCACCTACAGAATATAA

Protein sequence

MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI

Homology

[Close]

BLAST of Carg23546 vs. NCBI nr
Match: KAG6596756.1 (Transmembrane emp24 domain-containing protein p24beta2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028293.1 Transmembrane emp24 domain-containing protein p24beta2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 443.7 bits (1140), Expect = 9.2e-121
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVH 60
           MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVH
Sbjct: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVH 60

Query: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120
           TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD
Sbjct: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120

Query: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180
           IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM
Sbjct: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180

Query: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 224

[Close]

BLAST of Carg23546 vs. NCBI nr
Match: XP_022952151.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita moschata])

HSP 1 Score: 413.7 bits (1062), Expect = 1.0e-111
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

[Close]

BLAST of Carg23546 vs. NCBI nr
Match: XP_023005965.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita maxima])

HSP 1 Score: 411.0 bits (1055), Expect = 6.6e-111
Identity = 208/209 (99.52%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSV+TWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

[Close]

BLAST of Carg23546 vs. NCBI nr
Match: XP_023539502.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330.9 bits (847), Expect = 8.7e-87
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAK 176
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAK
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAK 160

[Close]

BLAST of Carg23546 vs. NCBI nr
Match: XP_022144018.1 (transmembrane emp24 domain-containing protein p24beta2-like [Momordica charantia])

HSP 1 Score: 285.0 bits (728), Expect = 5.5e-73
Identity = 151/224 (67.41%), Postives = 174/224 (77.68%), Query Frame = 0


Query: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVH 60
           M +G  K   R  +    + +S+  V G RFVIDKEECFSHNVEF RDTI+LSFVVVSVH
Sbjct: 1   MRIGKSKGWLRIAMVGLLVSVSAEAVFGFRFVIDKEECFSHNVEFTRDTIHLSFVVVSVH 60

Query: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120
               HAHNGVDL VKGP GE IEE +GKTSEKF+F++  KG Y FCF N SPH ETI+FD
Sbjct: 61  ASWRHAHNGVDLSVKGPKGEGIEEHKGKTSEKFKFVAHFKGPYRFCFTNKSPHHETIDFD 120

Query: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180
           IHI  Y+E YD D QHV++EHLSPL+ QI+KL E L ++EFEQQWLEAQTERQ KVNEAM
Sbjct: 121 IHITRYFEPYDHD-QHVQNEHLSPLMDQIEKLREALDIVEFEQQWLEAQTERQVKVNEAM 180

Query: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           S  AILKA+LESAALIGAS LQV LL RLFEK+VG L+S++Y I
Sbjct: 181 SRWAILKALLESAALIGASGLQVCLLCRLFEKRVGVLHSNSYTI 223

[Close]

BLAST of Carg23546 vs. ExPASy Swiss-Prot
Match: Q9S7M9 (Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana OX=3702 GN=At3g07680 PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 3.8e-53
Identity = 110/202 (54.46%), Postives = 142/202 (70.30%), Query Frame = 0


Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLL 73
           I +  L  S    LGIRFVID+EECFSH  E+  DT+++SFVV+   +  H   +GVDL+
Sbjct: 7   IVLLGLLWSFQATLGIRFVIDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLV 66

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           + GP GE+I + R + S K +F+  +KG Y FCF N SP+ ETI+FD+ + H + +YD  
Sbjct: 67  IHGPTGEQIHDFREQISAKHDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGH-FAYYD-- 126

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PL+ QI KL E L  I+FEQ WLEAQT+RQA VNE MS  A+ KA+ ES 
Sbjct: 127 -QHAKDEHFTPLMEQISKLEEALYNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESF 186

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           ALIGAS LQV+LLRRLFE+K+G
Sbjct: 187 ALIGASFLQVYLLRRLFERKLG 204

[Close]

BLAST of Carg23546 vs. ExPASy Swiss-Prot
Match: Q9LIL4 (Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana OX=3702 GN=At3g22845 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 9.2e-23
Identity = 67/209 (32.06%), Postives = 107/209 (51.20%), Query Frame = 0


Query: 7   KSKYRFFIFMAALFISS-HPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHH 66
           ++K   F+ +  + ++S + +  +   ++ EEC    V +  DT+  +FVVV    +   
Sbjct: 5   QAKIHVFVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHDIFWGS 64

Query: 67  AHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINH 126
            H G+D  V  P G  ++ ++G + +KFEF + + G Y FCF N     ET+ F IH+ H
Sbjct: 65  DHPGLDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFYIHVGH 124

Query: 127 YYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAI 186
                 ++    KDEHL P+  +I +L E L  +  EQ++L+A+  R    NE+     I
Sbjct: 125 ----IPNEHDLAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVI 184

Query: 187 LKAVLESAALIGASSLQVFLLRRLFEKKV 215
              V E   L  AS LQV  +R+LF K V
Sbjct: 185 FYTVGEYIFLAAASGLQVLYIRKLFSKSV 209

[Close]

BLAST of Carg23546 vs. ExPASy TrEMBL
Match: A0A6J1GJG3 (transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita moschata OX=3662 GN=LOC111454883 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 4.9e-112
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

[Close]

BLAST of Carg23546 vs. ExPASy TrEMBL
Match: A0A6J1KYW1 (transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita maxima OX=3661 GN=LOC111498823 PE=3 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 3.2e-111
Identity = 208/209 (99.52%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSV+TWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

[Close]

BLAST of Carg23546 vs. ExPASy TrEMBL
Match: A0A6J1CS53 (transmembrane emp24 domain-containing protein p24beta2-like OS=Momordica charantia OX=3673 GN=LOC111013807 PE=3 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.6e-73
Identity = 151/224 (67.41%), Postives = 174/224 (77.68%), Query Frame = 0


Query: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVH 60
           M +G  K   R  +    + +S+  V G RFVIDKEECFSHNVEF RDTI+LSFVVVSVH
Sbjct: 1   MRIGKSKGWLRIAMVGLLVSVSAEAVFGFRFVIDKEECFSHNVEFTRDTIHLSFVVVSVH 60

Query: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120
               HAHNGVDL VKGP GE IEE +GKTSEKF+F++  KG Y FCF N SPH ETI+FD
Sbjct: 61  ASWRHAHNGVDLSVKGPKGEGIEEHKGKTSEKFKFVAHFKGPYRFCFTNKSPHHETIDFD 120

Query: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180
           IHI  Y+E YD D QHV++EHLSPL+ QI+KL E L ++EFEQQWLEAQTERQ KVNEAM
Sbjct: 121 IHITRYFEPYDHD-QHVQNEHLSPLMDQIEKLREALDIVEFEQQWLEAQTERQVKVNEAM 180

Query: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           S  AILKA+LESAALIGAS LQV LL RLFEK+VG L+S++Y I
Sbjct: 181 SRWAILKALLESAALIGASGLQVCLLCRLFEKRVGVLHSNSYTI 223

[Close]

BLAST of Carg23546 vs. ExPASy TrEMBL
Match: A0A6J5XME6 (GOLD domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS34477 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.7e-59
Identity = 121/202 (59.90%), Postives = 153/202 (75.74%), Query Frame = 0


Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLL 73
           + +    +S   V GIRFVID+EECFSHNV+F  DT+++SFVV+ V    H+  +GVDL+
Sbjct: 11  VLLLTFLVSLQAVFGIRFVIDREECFSHNVQFEGDTVHISFVVIKVDASWHYTQDGVDLV 70

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           VKGP GE+I + R KTSEKF+F++  KG + FCF N SP+ ETI+FDIH++H + +YD  
Sbjct: 71  VKGPSGEQIHDFRDKTSEKFDFVTRHKGVHQFCFTNKSPYHETIDFDIHVSH-FTYYD-- 130

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PLL Q+ KL E L  I+FEQ WLEAQTERQA VNEAMS  AI KA  ESA
Sbjct: 131 -QHAKDEHFNPLLEQVSKLEEALYNIQFEQHWLEAQTERQAIVNEAMSRRAIHKAFFESA 190

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           AL+GAS+LQV+LLRRLFE+K+G
Sbjct: 191 ALVGASALQVYLLRRLFERKLG 208

[Close]

BLAST of Carg23546 vs. ExPASy TrEMBL
Match: M5WCT6 (GOLD domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G024700 PE=3 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 3.7e-59
Identity = 120/202 (59.41%), Postives = 153/202 (75.74%), Query Frame = 0


Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLL 73
           + +    +S   V GIRFVID+EECFSHNV++  DT+++SFVV+ V    H+  +GVDL+
Sbjct: 11  VLLLTFLVSLQAVFGIRFVIDREECFSHNVQYEGDTVHISFVVIKVDASWHYTQDGVDLV 70

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           VKGP GE+I + R KTSEKF+F++  KG + FCF N SP+ ETI+FDIH++H + +YD  
Sbjct: 71  VKGPSGEQIHDFRDKTSEKFDFVTRHKGVHQFCFTNKSPYHETIDFDIHVSH-FTYYD-- 130

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PLL Q+ KL E L  I+FEQ WLEAQTERQA VNEAMS  AI KA  ESA
Sbjct: 131 -QHAKDEHFNPLLEQVSKLEEALYNIQFEQHWLEAQTERQAIVNEAMSRRAIHKAFFESA 190

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           AL+GAS+LQV+LLRRLFE+K+G
Sbjct: 191 ALVGASALQVYLLRRLFERKLG 208

[Close]

BLAST of Carg23546 vs. TAIR 10
Match: AT3G07680.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 209.5 bits (532), Expect = 2.7e-54
Identity = 110/202 (54.46%), Postives = 142/202 (70.30%), Query Frame = 0


Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLL 73
           I +  L  S    LGIRFVID+EECFSH  E+  DT+++SFVV+   +  H   +GVDL+
Sbjct: 7   IVLLGLLWSFQATLGIRFVIDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLV 66

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           + GP GE+I + R + S K +F+  +KG Y FCF N SP+ ETI+FD+ + H + +YD  
Sbjct: 67  IHGPTGEQIHDFREQISAKHDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGH-FAYYD-- 126

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PL+ QI KL E L  I+FEQ WLEAQT+RQA VNE MS  A+ KA+ ES 
Sbjct: 127 -QHAKDEHFTPLMEQISKLEEALYNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESF 186

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           ALIGAS LQV+LLRRLFE+K+G
Sbjct: 187 ALIGASFLQVYLLRRLFERKLG 204

[Close]

BLAST of Carg23546 vs. TAIR 10
Match: AT3G22845.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 108.6 bits (270), Expect = 6.5e-24
Identity = 67/209 (32.06%), Postives = 107/209 (51.20%), Query Frame = 0


Query: 7   KSKYRFFIFMAALFISS-HPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHH 66
           ++K   F+ +  + ++S + +  +   ++ EEC    V +  DT+  +FVVV    +   
Sbjct: 5   QAKIHVFVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHDIFWGS 64

Query: 67  AHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINH 126
            H G+D  V  P G  ++ ++G + +KFEF + + G Y FCF N     ET+ F IH+ H
Sbjct: 65  DHPGLDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFYIHVGH 124

Query: 127 YYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAI 186
                 ++    KDEHL P+  +I +L E L  +  EQ++L+A+  R    NE+     I
Sbjct: 125 ----IPNEHDLAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVI 184

Query: 187 LKAVLESAALIGASSLQVFLLRRLFEKKV 215
              V E   L  AS LQV  +R+LF K V
Sbjct: 185 FYTVGEYIFLAAASGLQVLYIRKLFSKSV 209

The following BLAST results are available for this feature:

BLAST of Carg23546 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596756.1	9.2e-121	100.00	Transmembrane emp24 domain-containing protein p24beta2, partial [Cucurbita argyr...	[more]
XP_022952151.1	1.0e-111	100.00	transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita moschata]	[more]
XP_023005965.1	6.6e-111	99.52	transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita maxima]	[more]
XP_023539502.1	8.7e-87	100.00	transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita pepo subs...	[more]
XP_022144018.1	5.5e-73	67.41	transmembrane emp24 domain-containing protein p24beta2-like [Momordica charantia...	[more]

BLAST of Carg23546 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7M9	3.8e-53	54.46	Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana O...	[more]
Q9LIL4	9.2e-23	32.06	Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana O...	[more]

BLAST of Carg23546 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GJG3	4.9e-112	100.00	transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita moschat...	[more]
A0A6J1KYW1	3.2e-111	99.52	transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita maxima ...	[more]
A0A6J1CS53	2.6e-73	67.41	transmembrane emp24 domain-containing protein p24beta2-like OS=Momordica charant...	[more]
A0A6J5XME6	1.7e-59	59.90	GOLD domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS34...	[more]
M5WCT6	3.7e-59	59.41	GOLD domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G024700 PE=3 ...	[more]

BLAST of Carg23546 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07680.1	2.7e-54	54.46	emp24/gp25L/p24 family/GOLD family protein	[more]
AT3G22845.1	6.5e-24	32.06	emp24/gp25L/p24 family/GOLD family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009038	GOLD domain	SMART	SM01190	EMP24_GP25L_2	coord: 28..211 e-value: 9.2E-31 score: 118.2
IPR009038	GOLD domain	PFAM	PF01105	EMP24_GP25L	coord: 35..211 e-value: 5.8E-27 score: 94.8
IPR009038	GOLD domain	PROSITE	PS50866	GOLD	coord: 36..123 score: 11.698513
IPR015720	Transmembrane emp24 domain-containing protein	PANTHER	PTHR22811	TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN	coord: 25..215
None	No IPR available	PANTHER	PTHR22811:SF167	TMP21-RELATED PROTEIN-RELATED	coord: 25..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23546-RA	Carg23546-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport
biological_process	GO:0007030	Golgi organization
biological_process	GO:0006886	intracellular protein transport
cellular_component	GO:0030134	COPII-coated ER to Golgi transport vesicle
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005793	endoplasmic reticulum-Golgi intermediate compartment
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0016021	integral component of membrane