Carg23507 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23507
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProteasome subunit beta
LocationCarg_Chr14: 12749872 .. 12752411 (-)
RNA-Seq ExpressionCarg23507
SyntenyCarg23507
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGCGTATTTGGATTTGTTGGCAACGGATTCGTCATAGTGGCGGCTGACACATCGGCGGTGCATAGTATTTTGGTTCATAAATCCAACGAGGACAAGATCATGGTTCTCGATTCCCACAAGCTCGTTGCAGCTAGCGGCGAACCTGGCGACAGGTTTGAGGTTTTTGTTGATGGAAATCTTGTTTCTTCTCTAATCGTTTGCCTTTTGTTAATTATGTGTTTCTTTCGACTGTGATTCGATAGGGTTCAGTTCACGGAGTATATACAGAAGAATGTGGCTTTGTATCAGTTTCGCAATGGTATCCCTTTGACCTCTGCTGCTGCTGCTAATTTTACTCGCGGTGAACTCGCAACCGCCTTGCGGAAGGTAATATCATTTCTTCTTACTAAAACTTTTATTTGACTACATTTTCAACCTTTTAAGTTTTGGGAACTTTGGATCATGGGGGTTGTTTTAAGCGGTTCTAATAATTCATCGTGATGATGAAGTATGAATAGTTTTGTCTTCAATCTAACTTTTACTAGTTTGTGGAGCTTAGTGTTGGATGATGGAAGTCCCACATCGACTAATTTAGGGTGTGATCATGGGAAGCCCAAAGCAAAGTCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACATGGTATTAGAGCCATGCCCTAAACTCAGTCGTGCCACAATAGATTGGTAAATCCTCAAATATCGAACAAAGAACTCTTAAAGAAAAGGAGCCAAGCCCCTCCTCGAAGACAGTCAAAAAATGACTAAGACTCTAAAGGAGTCGAGCCTTGATTAAGGGGAGGTGCACTTTGTTCGAGGGGAGGTGTTGGATGATGGAAGTCCCACATCGACTAATTTAGGGTGTGATCATGGGTTTATAAGTGAGGAATACTACCTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGTAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACACTTAGAACTGTTCAAATGTTTACCGAGCGAGGGAATATTGTCTTGGTTTGTTTTTCTCGAAACCCCTTGATTTCTGTCATTATGTCGTCTTGAAGGATGCCTCACAATGGAAAGAAACTAGACCGATAACCATCTCCGGATGTTGTATGTTAAACACTTTGATTTGCGTTTTTGTTCTTGTATCCAGCTGTGTTGAGTGTAGGCAGGCCAAAGTTCATGTATTTCTCTTATATCATGTTTCGTCTTGCTATTATCATCATCCTATTGGTTCATTTCTTAGTATTAAGGATGGCATTTCATAATCCAGAAAGTTAACAATGATGTGTATTGTACTCTACTTCTTGCCTGAGAAATAGAACCAAGCCCACCGATAGATATTGTCATCTCTGAGTTTTCTCTTTAAGGACTTCTCTGGAAGGGTTTAAAATGCGGCTGTTAGAGAAGGGTTTTCACACTCTTATAATGAATGTTTCGTACCTTTATAAGGAATGATTTGTTTCCTTCTCCAACCGATATGGGATCTCATAATCTACTCCCTTGGTGGCCAGTGCCTCGCTGGCACTCCACCCGATGTCTGGCTCTGATACCATTTGTAACAGCTCAATCCCATTATTAGTAGATAGTCCTTGTAACCTCATTTAATTTCACTAAATTCTGCTAGAAATGTGTTCGAATGTAATTTGATGTGTTGGTGGGCGAGATCGGGTTGGCTTTATAATGGTATGGCATGTGATGTGGTGATGACTGCATAGAATACTAATGACTTTCATGAACGCTGGTTCGTGTTTACAAATGTACACTCTTATGTCTAGTTCTATAAAATCTTCCTTGATCTTGCTCCCCTAGCGTCACCCTCCCCTAGCGTGCACAAGGGTGACGTCTCTTACTGAGTTGTCTGCTTCTCAAAGTACCAGTTATCATGTTTCACCACTACAACTAAGTTTTTAGCCACTGGGGCACAAGTCTTTGTTCATTAAGGATAAGCTAGGCTCGATGTAGAACTCGTGTGAATAACGAGCCCATAAAAGAGGAGTTTTACTGTGTAATTTGGTTTTTGTGCTTAGAACATAATAATAAGTGATCCACTATTCTTCTTGTAATCCTGCATTTAGCTGTATCTTTGAATGAGCAACTGTCTCCTGATTTCAATTGTGAGTTCTTGATTTTCAGAATCCATATTCTGTGAACATTCTTTTGGCGGGATATGACAAGGAGACAGGACCATCCCTGTTTTACATTGACTATATTGCCACCCTTCACAAGGTTGACAAGGGAGCTTTTGGTTATGGATCTTACTTTTCACTCTCCATGATGGATAGACACTACCATAGTGGTATGACAGAAGAAGAAGCTATTGATTTGGTTGATAAATGCATACTTGAGATCAGGTCCAGGCTTGTTGTGGCCCCACCGAACTTCGTTATCAAAATTGTAGACAAGAATGGAGCTAGGGAGGTTGCTTGGCGTCAATCCATCAAGGATGGAGCTAGCGTTCCTTCAGCTTAA

mRNA sequence

ATGGAGAGCGTATTTGGATTTGTTGGCAACGGATTCGTCATAGTGGCGGCTGACACATCGGCGGTGCATAGTATTTTGGTTCATAAATCCAACGAGGACAAGATCATGGTTCTCGATTCCCACAAGCTCGTTGCAGCTAGCGGCGAACCTGGCGACAGGGTTCAGTTCACGGAGTATATACAGAAGAATGTGGCTTTGTATCAGTTTCGCAATGGTATCCCTTTGACCTCTGCTGCTGCTGCTAATTTTACTCGCGGTGAACTCGCAACCGCCTTGCGGAAGAATCCATATTCTGTGAACATTCTTTTGGCGGGATATGACAAGGAGACAGGACCATCCCTGTTTTACATTGACTATATTGCCACCCTTCACAAGGTTGACAAGGGAGCTTTTGGTTATGGATCTTACTTTTCACTCTCCATGATGGATAGACACTACCATAGTGGTATGACAGAAGAAGAAGCTATTGATTTGGTTGATAAATGCATACTTGAGATCAGGTCCAGGCTTGTTGTGGCCCCACCGAACTTCGTTATCAAAATTGTAGACAAGAATGGAGCTAGGGAGGTTGCTTGGCGTCAATCCATCAAGGATGGAGCTAGCGTTCCTTCAGCTTAA

Coding sequence (CDS)

ATGGAGAGCGTATTTGGATTTGTTGGCAACGGATTCGTCATAGTGGCGGCTGACACATCGGCGGTGCATAGTATTTTGGTTCATAAATCCAACGAGGACAAGATCATGGTTCTCGATTCCCACAAGCTCGTTGCAGCTAGCGGCGAACCTGGCGACAGGGTTCAGTTCACGGAGTATATACAGAAGAATGTGGCTTTGTATCAGTTTCGCAATGGTATCCCTTTGACCTCTGCTGCTGCTGCTAATTTTACTCGCGGTGAACTCGCAACCGCCTTGCGGAAGAATCCATATTCTGTGAACATTCTTTTGGCGGGATATGACAAGGAGACAGGACCATCCCTGTTTTACATTGACTATATTGCCACCCTTCACAAGGTTGACAAGGGAGCTTTTGGTTATGGATCTTACTTTTCACTCTCCATGATGGATAGACACTACCATAGTGGTATGACAGAAGAAGAAGCTATTGATTTGGTTGATAAATGCATACTTGAGATCAGGTCCAGGCTTGTTGTGGCCCCACCGAACTTCGTTATCAAAATTGTAGACAAGAATGGAGCTAGGGAGGTTGCTTGGCGTCAATCCATCAAGGATGGAGCTAGCGTTCCTTCAGCTTAA

Protein sequence

MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYIQKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYIATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIKIVDKNGAREVAWRQSIKDGASVPSA
Homology
[Close]
BLAST of Carg23507 vs. NCBI nr
Match: XP_022956298.1 (proteasome subunit beta type-2-A-like [Cucurbita moschata] >XP_022979470.1 proteasome subunit beta type-2-A-like [Cucurbita maxima] >KAG6582311.1 Proteasome subunit beta type-2-A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018723.1 Proteasome subunit beta type-2-A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 407.9 bits (1047), Expect = 5.1e-110
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI
Sbjct: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKDGASVPSA 206
           IVDKNGAREVAWRQSIKDGASVPSA
Sbjct: 181 IVDKNGAREVAWRQSIKDGASVPSA 205

[Close]
BLAST of Carg23507 vs. NCBI nr
Match: XP_023527188.1 (proteasome subunit beta type-2-A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 406.4 bits (1043), Expect = 1.5e-109
Identity = 204/205 (99.51%), Postives = 204/205 (99.51%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI
Sbjct: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           AT HKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATFHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKDGASVPSA 206
           IVDKNGAREVAWRQSIKDGASVPSA
Sbjct: 181 IVDKNGAREVAWRQSIKDGASVPSA 205

[Close]
BLAST of Carg23507 vs. NCBI nr
Match: XP_038875710.1 (proteasome subunit beta type-2-A-like [Benincasa hispida])

HSP 1 Score: 399.4 bits (1025), Expect = 1.8e-107
Identity = 199/205 (97.07%), Postives = 203/205 (99.02%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFG VGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNVALYQFRNGIPLT+AAAANFTRGELATALRKNPYSVNILLAGYDKETGPSL+YIDYI
Sbjct: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVDKGAFGYGSYFSL+MMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKDGASVPSA 206
           IVDKNGAREVAWR+SIKDG SVPSA
Sbjct: 181 IVDKNGAREVAWRESIKDGVSVPSA 205

[Close]
BLAST of Carg23507 vs. NCBI nr
Match: XP_008438650.1 (PREDICTED: proteasome subunit beta type-2-A [Cucumis melo] >KAA0049346.1 proteasome subunit beta type-2-A [Cucumis melo var. makuwa] >TYK17212.1 proteasome subunit beta type-2-A [Cucumis melo var. makuwa])

HSP 1 Score: 397.5 bits (1020), Expect = 6.9e-107
Identity = 197/205 (96.10%), Postives = 203/205 (99.02%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFG VGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNVALYQFRNGIPLT+AAAANFTRGELATALRKNPYSVN+LLAGYDKETGPSL+YIDYI
Sbjct: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNVLLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKV+KGAFGYGSYFSL+MMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKDGASVPSA 206
           IVDKNGAREVAWR+SIKDG SVPSA
Sbjct: 181 IVDKNGAREVAWRESIKDGVSVPSA 205

[Close]
BLAST of Carg23507 vs. NCBI nr
Match: XP_022138279.1 (proteasome subunit beta type-2-A-like [Momordica charantia])

HSP 1 Score: 397.1 bits (1019), Expect = 9.0e-107
Identity = 197/205 (96.10%), Postives = 203/205 (99.02%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFG VGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MECVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNV+LYQFRNGIPLT+AAAANFTRGELATALRKNPYSVNILLAGYDKETGPSL+YIDYI
Sbjct: 61  QKNVSLYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKDGASVPSA 206
           I+D+NGAREVAWR+SIKDGASVPSA
Sbjct: 181 IIDENGAREVAWRESIKDGASVPSA 205

[Close]
BLAST of Carg23507 vs. ExPASy Swiss-Prot
Match: O23714 (Proteasome subunit beta type-2-A OS=Arabidopsis thaliana OX=3702 GN=PBD1 PE=1 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 6.7e-97
Identity = 174/198 (87.88%), Postives = 188/198 (94.95%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFG VGNGF IVAADTSAVHSIL+HK+NEDKIMVLDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1   MECVFGLVGNGFAIVAADTSAVHSILLHKNNEDKIMVLDSHKLVAASGEPGDRVQFTEYV 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNV+LY+FRNGIPLT+AAAANFTRGELATALRKNPYSVNIL+AGYD E+G SL+YIDYI
Sbjct: 61  QKNVSLYKFRNGIPLTTAAAANFTRGELATALRKNPYSVNILMAGYDDESGASLYYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVDKGAFGYGSYFSLS MDRHY S M+ EEAI+LVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKD 199
           IVDK+GAR+ AWRQS+KD
Sbjct: 181 IVDKDGARDYAWRQSVKD 198

[Close]
BLAST of Carg23507 vs. ExPASy Swiss-Prot
Match: O24633 (Proteasome subunit beta type-2-B OS=Arabidopsis thaliana OX=3702 GN=PBD2 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 5.3e-94
Identity = 170/198 (85.86%), Postives = 182/198 (91.92%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFG VGNGF IVAADTSAVHSIL+HK+ EDKIM LDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1   MECVFGLVGNGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYV 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNV+LYQFRNGIPL++AAAANFTRGELATALRKNPYSVNIL+AGYDKE G SL+YIDYI
Sbjct: 61  QKNVSLYQFRNGIPLSTAAAANFTRGELATALRKNPYSVNILMAGYDKEAGASLYYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVDKGAFGYGSYFSLS MDRHY S M+ EEAI+LVDKCILEIRSRLV+APPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVIAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKD 199
           IVDK+GARE  WR S  D
Sbjct: 181 IVDKDGAREYGWRISTAD 198

[Close]
BLAST of Carg23507 vs. ExPASy Swiss-Prot
Match: Q9LST6 (Proteasome subunit beta type-2 OS=Oryza sativa subsp. japonica OX=39947 GN=PBD1 PE=2 SV=2)

HSP 1 Score: 297.4 bits (760), Expect = 1.3e-79
Identity = 146/193 (75.65%), Postives = 165/193 (85.49%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME V G VG  F +VAADTSAV SILVHK++EDK+MVLDSHKL+ ASGEPGDRVQFTE+I
Sbjct: 1   MECVIGVVGRDFAVVAADTSAVQSILVHKTDEDKVMVLDSHKLMGASGEPGDRVQFTEFI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKN+ LYQFRN IPL++AA ANFTRGELATALRKNPY VN+LLAGYD + G SL+YIDYI
Sbjct: 61  QKNLHLYQFRNNIPLSTAATANFTRGELATALRKNPYYVNVLLAGYDSDVGASLYYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           AT HK++KGAFGYGSYF LS+MD+ Y   M+ EEA+DLVDKCI EIR RLVVAP NF+IK
Sbjct: 121 ATFHKIEKGAFGYGSYFCLSLMDKLYRPDMSVEEAVDLVDKCIKEIRLRLVVAPQNFIIK 180

Query: 181 IVDKNGAREVAWR 194
           IVDK GARE A R
Sbjct: 181 IVDKEGAREYARR 193

[Close]
BLAST of Carg23507 vs. ExPASy Swiss-Prot
Match: Q55DY7 (Proteasome subunit beta type-2 OS=Dictyostelium discoideum OX=44689 GN=psmB2 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 4.2e-46
Identity = 89/191 (46.60%), Postives = 132/191 (69.11%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME++  F   G V+  AD+S  HSI+  K +EDKI+ +D HKL+ ++GE GDRVQFTEYI
Sbjct: 1   METLLAFKVKGGVLCLADSSVNHSIVKMKDDEDKILEIDGHKLLLSAGEAGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKET---GPSLFYI 120
            KN+ LY  RN  P+++ AAANF R ELAT++R +PYS+N++LAGYDKE    G SL+Y+
Sbjct: 61  SKNIKLYSLRNSYPMSTPAAANFIRNELATSIRSHPYSINLILAGYDKEVEDGGTSLYYM 120

Query: 121 DYIATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNF 180
           DY+ +L K++ G  GY SYF L ++DRH+   ++ E+ I+L+  C  E+++R +V+   +
Sbjct: 121 DYLGSLQKLNFGCHGYASYFLLGLLDRHHKCDLSLEDGINLMHLCTTELKTRFLVS-GKY 180

Query: 181 VIKIVDKNGAR 189
            +K V   G +
Sbjct: 181 TLKFVSSEGIK 190

[Close]
BLAST of Carg23507 vs. ExPASy Swiss-Prot
Match: P49721 (Proteasome subunit beta type-2 OS=Homo sapiens OX=9606 GN=PSMB2 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 8.7e-44
Identity = 87/191 (45.55%), Postives = 127/191 (66.49%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME + G  G  +V+VA+D  A  +I+  K + DK+  +    L+   GE GD VQF EYI
Sbjct: 1   MEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSEKILLLCVGEAGDTVQFAEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALR-KNPYSVNILLAGYDKETGPSLFYIDY 120
           QKNV LY+ RNG  L+  AAANFTR  LA  LR + PY VN+LLAGYD+  GP+L+Y+DY
Sbjct: 61  QKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDY 120

Query: 121 IATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVI 180
           +A L K    A GYG++ +LS++DR+Y   ++ E A++L+ KC+ E++ R ++  P F +
Sbjct: 121 LAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSV 180

Query: 181 KIVDKNGAREV 191
           +I+DKNG  ++
Sbjct: 181 RIIDKNGIHDL 191

[Close]
BLAST of Carg23507 vs. ExPASy TrEMBL
Match: A0A6J1ITC5 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111479173 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.5e-110
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI
Sbjct: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKDGASVPSA 206
           IVDKNGAREVAWRQSIKDGASVPSA
Sbjct: 181 IVDKNGAREVAWRQSIKDGASVPSA 205

[Close]
BLAST of Carg23507 vs. ExPASy TrEMBL
Match: A0A6J1GWE4 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111458036 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.5e-110
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI
Sbjct: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKDGASVPSA 206
           IVDKNGAREVAWRQSIKDGASVPSA
Sbjct: 181 IVDKNGAREVAWRQSIKDGASVPSA 205

[Close]
BLAST of Carg23507 vs. ExPASy TrEMBL
Match: A0A5D3D072 (Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00590 PE=3 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 3.4e-107
Identity = 197/205 (96.10%), Postives = 203/205 (99.02%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFG VGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNVALYQFRNGIPLT+AAAANFTRGELATALRKNPYSVN+LLAGYDKETGPSL+YIDYI
Sbjct: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNVLLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKV+KGAFGYGSYFSL+MMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKDGASVPSA 206
           IVDKNGAREVAWR+SIKDG SVPSA
Sbjct: 181 IVDKNGAREVAWRESIKDGVSVPSA 205

[Close]
BLAST of Carg23507 vs. ExPASy TrEMBL
Match: A0A1S3AXK5 (Proteasome subunit beta OS=Cucumis melo OX=3656 GN=LOC103483693 PE=3 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 3.4e-107
Identity = 197/205 (96.10%), Postives = 203/205 (99.02%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFG VGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNVALYQFRNGIPLT+AAAANFTRGELATALRKNPYSVN+LLAGYDKETGPSL+YIDYI
Sbjct: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNVLLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKV+KGAFGYGSYFSL+MMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKDGASVPSA 206
           IVDKNGAREVAWR+SIKDG SVPSA
Sbjct: 181 IVDKNGAREVAWRESIKDGVSVPSA 205

[Close]
BLAST of Carg23507 vs. ExPASy TrEMBL
Match: A0A6J1C9Q2 (Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111009495 PE=3 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 4.4e-107
Identity = 197/205 (96.10%), Postives = 203/205 (99.02%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFG VGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MECVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNV+LYQFRNGIPLT+AAAANFTRGELATALRKNPYSVNILLAGYDKETGPSL+YIDYI
Sbjct: 61  QKNVSLYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKDGASVPSA 206
           I+D+NGAREVAWR+SIKDGASVPSA
Sbjct: 181 IIDENGAREVAWRESIKDGASVPSA 205

[Close]
BLAST of Carg23507 vs. TAIR 10
Match: AT3G22630.1 (20S proteasome beta subunit D1 )

HSP 1 Score: 354.8 bits (909), Expect = 4.8e-98
Identity = 174/198 (87.88%), Postives = 188/198 (94.95%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFG VGNGF IVAADTSAVHSIL+HK+NEDKIMVLDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1   MECVFGLVGNGFAIVAADTSAVHSILLHKNNEDKIMVLDSHKLVAASGEPGDRVQFTEYV 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNV+LY+FRNGIPLT+AAAANFTRGELATALRKNPYSVNIL+AGYD E+G SL+YIDYI
Sbjct: 61  QKNVSLYKFRNGIPLTTAAAANFTRGELATALRKNPYSVNILMAGYDDESGASLYYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVDKGAFGYGSYFSLS MDRHY S M+ EEAI+LVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKD 199
           IVDK+GAR+ AWRQS+KD
Sbjct: 181 IVDKDGARDYAWRQSVKD 198

[Close]
BLAST of Carg23507 vs. TAIR 10
Match: AT4G14800.1 (20S proteasome beta subunit D2 )

HSP 1 Score: 345.1 bits (884), Expect = 3.8e-95
Identity = 170/198 (85.86%), Postives = 182/198 (91.92%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFG VGNGF IVAADTSAVHSIL+HK+ EDKIM LDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1   MECVFGLVGNGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYV 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLFYIDYI 120
           QKNV+LYQFRNGIPL++AAAANFTRGELATALRKNPYSVNIL+AGYDKE G SL+YIDYI
Sbjct: 61  QKNVSLYQFRNGIPLSTAAAANFTRGELATALRKNPYSVNILMAGYDKEAGASLYYIDYI 120

Query: 121 ATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVDKGAFGYGSYFSLS MDRHY S M+ EEAI+LVDKCILEIRSRLV+APPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVIAPPNFVIK 180

Query: 181 IVDKNGAREVAWRQSIKD 199
           IVDK+GARE  WR S  D
Sbjct: 181 IVDKDGAREYGWRISTAD 198

[Close]
BLAST of Carg23507 vs. TAIR 10
Match: AT4G14800.2 (20S proteasome beta subunit D2 )

HSP 1 Score: 334.7 bits (857), Expect = 5.1e-92
Identity = 170/214 (79.44%), Postives = 182/214 (85.05%), Query Frame = 0 

Query: 1   MESVFGFVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFG VGNGF IVAADTSAVHSIL+HK+ EDKIM LDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1   MECVFGLVGNGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYV 60

Query: 61  QKNVALYQFRNGIPLTSAAAANFTRGELATALRK----------------NPYSVNILLA 120
           QKNV+LYQFRNGIPL++AAAANFTRGELATALRK                NPYSVNIL+A
Sbjct: 61  QKNVSLYQFRNGIPLSTAAAANFTRGELATALRKEFCATFQGYSDFCVLMNPYSVNILMA 120

Query: 121 GYDKETGPSLFYIDYIATLHKVDKGAFGYGSYFSLSMMDRHYHSGMTEEEAIDLVDKCIL 180
           GYDKE G SL+YIDYIATLHKVDKGAFGYGSYFSLS MDRHY S M+ EEAI+LVDKCIL
Sbjct: 121 GYDKEAGASLYYIDYIATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCIL 180

Query: 181 EIRSRLVVAPPNFVIKIVDKNGAREVAWRQSIKD 199
           EIRSRLV+APPNFVIKIVDK+GARE  WR S  D
Sbjct: 181 EIRSRLVIAPPNFVIKIVDKDGAREYGWRISTAD 214

The following BLAST results are available for this feature:
BLAST of Carg23507 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022956298.15.1e-110100.00proteasome subunit beta type-2-A-like [Cucurbita moschata] >XP_022979470.1 prote... [more]
XP_023527188.11.5e-10999.51proteasome subunit beta type-2-A-like [Cucurbita pepo subsp. pepo][more]
XP_038875710.11.8e-10797.07proteasome subunit beta type-2-A-like [Benincasa hispida][more]
XP_008438650.16.9e-10796.10PREDICTED: proteasome subunit beta type-2-A [Cucumis melo] >KAA0049346.1 proteas... [more]
XP_022138279.19.0e-10796.10proteasome subunit beta type-2-A-like [Momordica charantia][more]
BLAST of Carg23507 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O237146.7e-9787.88Proteasome subunit beta type-2-A OS=Arabidopsis thaliana OX=3702 GN=PBD1 PE=1 SV... [more]
O246335.3e-9485.86Proteasome subunit beta type-2-B OS=Arabidopsis thaliana OX=3702 GN=PBD2 PE=1 SV... [more]
Q9LST61.3e-7975.65Proteasome subunit beta type-2 OS=Oryza sativa subsp. japonica OX=39947 GN=PBD1 ... [more]
Q55DY74.2e-4646.60Proteasome subunit beta type-2 OS=Dictyostelium discoideum OX=44689 GN=psmB2 PE=... [more]
P497218.7e-4445.55Proteasome subunit beta type-2 OS=Homo sapiens OX=9606 GN=PSMB2 PE=1 SV=1[more]
BLAST of Carg23507 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1ITC52.5e-110100.00Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111479173 PE=3 SV=1[more]
A0A6J1GWE42.5e-110100.00Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111458036 PE=3 SV=1[more]
A0A5D3D0723.4e-10796.10Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3AXK53.4e-10796.10Proteasome subunit beta OS=Cucumis melo OX=3656 GN=LOC103483693 PE=3 SV=1[more]
A0A6J1C9Q24.4e-10796.10Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111009495 PE=3 SV=1[more]
BLAST of Carg23507 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G22630.14.8e-9887.8820S proteasome beta subunit D1 [more]
AT4G14800.13.8e-9585.8620S proteasome beta subunit D2 [more]
AT4G14800.25.1e-9279.4420S proteasome beta subunit D2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001353Proteasome, subunit alpha/betaPFAMPF00227Proteasomecoord: 3..182
e-value: 4.0E-40
score: 137.3
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 1..192
e-value: 7.8E-54
score: 184.3
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 1..190
NoneNo IPR availablePANTHERPTHR11599:SF181PROTEASOME SUBUNIT BETA TYPE-2-Bcoord: 1..199
NoneNo IPR availablePANTHERPTHR11599PROTEASOME SUBUNIT ALPHA/BETAcoord: 1..199
IPR016050Proteasome beta-type subunit, conserved sitePROSITEPS00854PROTEASOME_BETA_1coord: 5..52
IPR023333Proteasome B-type subunitPROSITEPS51476PROTEASOME_BETA_2coord: 1..182
score: 42.787254
IPR035206Proteasome subunit beta 2CDDcd03758proteasome_beta_type_2coord: 1..192
e-value: 7.81326E-116
score: 325.694

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23507-RACarg23507-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010498 proteasomal protein catabolic process
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0019774 proteasome core complex, beta-subunit complex
cellular_component GO:0005839 proteasome core complex
molecular_function GO:0004298 threonine-type endopeptidase activity