Carg23481 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23481
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	CASP-like protein
Location	Carg_Chr12: 9699310 .. 9702229 (-)
RNA-Seq Expression	Carg23481
Synteny	Carg23481

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCGGTGGCAATGGGGTTTTGGGATCAAAGAACTGAAGGGATTCTCAGATCCTTTGCGATCATTGGCTTGGTTTTAACTGTTCTTTTGTTGGGTTTTGATAAGCAAACTACCCAAATCTTTCATATTGACAAGAAGGCCTCTTTCAGAAGTTTGCATGCTTTTGTGTAAGCCCTTTCTCTCTCATTTTTCAAACTCTTTCTCGTTTTCATTCTTGGCCTTTTAGGTTTCGTGTTTTAAAATTTTGATGAACCGGAGATTTGAACTCTGAGATTCCACATCGATTGGAGATGAAAATGAAACACCCTTTATAAGAGTGTGGAAACCTTTCCGTAACAACGCATTTTAAAAACCTTGAGGGGATGTCCGAGAAGGAAAGCCCAAAAAGGACAATATTTGCTAGCCGTGGGCTTGAGCTGTTACGAATAGTATCAGGGTCAGATACCGGACGATATGGCAGGTAGAAGGTTGTAACCTGAAGCGGGTAGACATGAGGCAATGTGTCAGCAAAAACACTGGGTCTCGAAGGGGATGGATTTTGTGGAGGTCTCACATTGACTAAAGAAATGAACGAGTGCCAACGAGGACGCTAGGCTCTGAAAGGCGGTGAATTGTGAGATTCCACATTGGTTGGGGAGGATAATAAAAGGTTGTTCCCCGAAGGAGGTAGACACGAGACGATGTGCCAGTAAGGATGTTGGGTCTCGAGGGGGATTTGGTGGGGTCTCACATCAATTGGAGAAAGGCTTGAGTGCCAGCAAGGACACTGGGTCCTAAAGGGTTGAATTGTGAGGTTCCACATCGATTGGGGAGGAACGAAACACTCTTTACACGAACATCTAAGTTTCAATGATAAATAACAAATGTCAAAAGTTTCATTGGATGACCAATGAAACGAAACTCGTGAAAGAACATTTCTAAGTTAAACTTTCAGAAATCAAATGATTATCGAACCGAATTTAAAGTTATTCAATTTTCTTGCCATTGGTGACAGAGTGATTATGTATGTGGACTCCCTAGCAGCTGCCTACAACATTCTTCAAATCTGCAAATGTTGGATTTCAGCTCAGCCAAAAGGAACATCCAAATTAGGGAGTGGTTCTCATATATGCCTATTTTGGGCTTCTTTCTTGCTTGACCAGGTATGTCTTAATATCTCTTTTACTTATCTTCAAATTTTTAGTACTTCTATTGATAAATTTAGGTCTAAAAATCTCGAATTTTTGTAAGTCAATTATCAAATTTATATAGTTAGTGAGACGAGCGGACTAGACTCGTCTTACAAACTTAGGTTACATGAATCTAATCATCAAACCAAAAGAATTTAAAGATTATCACGAGTTTCTATCTCTTCCTATATTAAATGTAATAGCCTAATCCTACTTAGATATTGTCTGCTTTAATCCATTACGTATAACCGTTAAATTTACCGTCTTTAACAGTAGTAAGGTTCGTAAAAGATTCAAAGAAAAATGTTTTTAATAGCTCTAATTTGGGGTAGATGGACTGTTCCAACCTATGGCTATGGAGGAAAAGGACACCGACAAGTGGACAAGAAACCAGTTGATGTAACTAACAAACATTTAAGAAAGTTCTAAGCTATGTTAGACTTTGAAAACTTTTCATAACCAAATCCAAACATTTAAATTTAATCTCAACATCTTAATATTTTTTCTAATTGTACATAATGTTTAAATTAGTTCATAATACACTTACGAGTATCCTACTTCAGATGAAAAATTAGGTTACTTATTCGAAGAAGATTTATCAGTTTAAAAAAAAATTAAAATTTGAGAGAACAAGAACAAATTAATCAATCTCGAGAAAGTGAAAATCTTGTCTACTTTTAACCATTTGAGCTTAGTCTCAAGATTTTCTTTATTAGTGGGTTCTACCCATAATTATTGAACTCCTTTCCTTTAAACTACAATCTTTCAGACAAAATCATAGCGGAACTAGTTCTCCTTTAACTATTATTGGAAAAGACCTTCTTTGGGGTGTAAGGCGTAAATGTCGACTTGATTTCTAATTCAGAGAGAGAAGTATATTCCTTATGCTCTAGGACATTCATCCATTACTCTCGAGTGTATTTTTCTTGAGCTAATCCTCTTTCAGAAGCTCGAACAGTTTGAGTGTTAGATATAGGATCACGATAGCATTATCAATAGCAGTACTTAGTAAATTCTTATATAATCCTCGAAAGATATGAAATTTTATTGCGATTCATGGGAAAAAAAAAATAGAATTTGACGAGAATATTTTTCATTTAATGAAAATCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTGACTATTAATACATAAATTATATAGAAACAGGTCAGGAATTTGAATCTTCACTTTTGTCGGGTCCATCTCGAACTCGAGAAAGGACAAAACTTATAACAAATAAATAAAAAGTTGTCTCAACCATGGGTCCAAACCGTCCCAAACATGTTTTATTATGTGCCAACATCCAACTTAAAAAGTGGTTTGAACTGGCATTGACAGGCAGTGGCGTACCTCGTATTTGCCGCAAACACGGCGGGGATGGAGGCGGCGCTTCTGGCGGTGACCGGCGCACATGACTTTCAATGGATGAAGCTCTGCAACAGATTCGAGAGATTCTGCTACCAAGTCGGCGGAGCTTTTCTTTGTGGCTATGGAGCTTTTTTTGCAATGTGTGCCATTTGCGTTATCTCTTCTTTCAACTTGTTTAGGCATTATTCCCCAAACCTTTTCTTTCGACTAAAATCCAACGAGTGAATTGTTCGATATCGACTTCGAGACCTTGTAACTTTACCAAGTCTATGAATTGTATTCATATCTGTATGTCGAAGACATTTGGATCAATACACACTATCTTTATTAAAAATGAGATCTTTTGAATAGGGTTTGAATAGAATAATAAGATATG

mRNA sequence

AGCGGTGGCAATGGGGTTTTGGGATCAAAGAACTGAAGGGATTCTCAGATCCTTTGCGATCATTGGCTTGGTTTTAACTGTTCTTTTGTTGGGTTTTGATAAGCAAACTACCCAAATCTTTCATATTGACAAGAAGGCCTCTTTCAGAAGTTTGCATGCTTTTGTAGTGATTATGTATGTGGACTCCCTAGCAGCTGCCTACAACATTCTTCAAATCTGCAAATGTTGGATTTCAGCTCAGCCAAAAGGAACATCCAAATTAGGGAGTGGTTCTCATATATGCCTATTTTGGGCTTCTTTCTTGCTTGACCAGGCAGTGGCGTACCTCGTATTTGCCGCAAACACGGCGGGGATGGAGGCGGCGCTTCTGGCGGTGACCGGCGCACATGACTTTCAATGGATGAAGCTCTGCAACAGATTCGAGAGATTCTGCTACCAAGTCGGCGGAGCTTTTCTTTGTGGCTATGGAGCTTTTTTTGCAATGTGTGCCATTTGCGTTATCTCTTCTTTCAACTTGTTTAGGCATTATTCCCCAAACCTTTTCTTTCGACTAAAATCCAACGAGTGAATTGTTCGATATCGACTTCGAGACCTTGTAACTTTACCAAGTCTATGAATTGTATTCATATCTGTATGTCGAAGACATTTGGATCAATACACACTATCTTTATTAAAAATGAGATCTTTTGAATAGGGTTTGAATAGAATAATAAGATATG

Coding sequence (CDS)

ATGGGGTTTTGGGATCAAAGAACTGAAGGGATTCTCAGATCCTTTGCGATCATTGGCTTGGTTTTAACTGTTCTTTTGTTGGGTTTTGATAAGCAAACTACCCAAATCTTTCATATTGACAAGAAGGCCTCTTTCAGAAGTTTGCATGCTTTTGTAGTGATTATGTATGTGGACTCCCTAGCAGCTGCCTACAACATTCTTCAAATCTGCAAATGTTGGATTTCAGCTCAGCCAAAAGGAACATCCAAATTAGGGAGTGGTTCTCATATATGCCTATTTTGGGCTTCTTTCTTGCTTGACCAGGCAGTGGCGTACCTCGTATTTGCCGCAAACACGGCGGGGATGGAGGCGGCGCTTCTGGCGGTGACCGGCGCACATGACTTTCAATGGATGAAGCTCTGCAACAGATTCGAGAGATTCTGCTACCAAGTCGGCGGAGCTTTTCTTTGTGGCTATGGAGCTTTTTTTGCAATGTGTGCCATTTGCGTTATCTCTTCTTTCAACTTGTTTAGGCATTATTCCCCAAACCTTTTCTTTCGACTAAAATCCAACGAGTGA

Protein sequence

MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSLAAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALLAVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFRLKSNE

Homology

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BLAST of Carg23481 vs. NCBI nr
Match: XP_023537147.1 (CASP-like protein 2C1 [Cucurbita pepo subsp. pepo] >KAG6586191.1 CASP-like protein 2C1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021022.1 CASP-like protein 2C1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 379.0 bits (972), Expect = 2.3e-101
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60
           MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL
Sbjct: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60

Query: 61  AAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120
           AAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL
Sbjct: 61  AAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120

Query: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180
           AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR
Sbjct: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180

Query: 181 LKSNE 186
           LKSNE
Sbjct: 181 LKSNE 185

[Close]

BLAST of Carg23481 vs. NCBI nr
Match: XP_022938059.1 (CASP-like protein 2C1 [Cucurbita moschata])

HSP 1 Score: 377.1 bits (967), Expect = 8.7e-101
Identity = 184/185 (99.46%), Postives = 184/185 (99.46%), Query Frame = 0


Query: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60
           MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL
Sbjct: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60

Query: 61  AAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120
           AAAYNILQICKCWISA PKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL
Sbjct: 61  AAAYNILQICKCWISAHPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120

Query: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180
           AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR
Sbjct: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180

Query: 181 LKSNE 186
           LKSNE
Sbjct: 181 LKSNE 185

[Close]

BLAST of Carg23481 vs. NCBI nr
Match: XP_022965970.1 (CASP-like protein 2C1 [Cucurbita maxima])

HSP 1 Score: 373.6 bits (958), Expect = 9.7e-100
Identity = 182/185 (98.38%), Postives = 183/185 (98.92%), Query Frame = 0


Query: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60
           MGFWDQ+TEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL
Sbjct: 1   MGFWDQKTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60

Query: 61  AAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120
           AAAYNILQIC CWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL
Sbjct: 61  AAAYNILQICNCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120

Query: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180
           AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHY PNLFFR
Sbjct: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYLPNLFFR 180

Query: 181 LKSNE 186
           LKSNE
Sbjct: 181 LKSNE 185

[Close]

BLAST of Carg23481 vs. NCBI nr
Match: XP_004149074.1 (CASP-like protein 2C1 [Cucumis sativus] >KGN61092.1 hypothetical protein Csa_021217 [Cucumis sativus])

HSP 1 Score: 292.7 bits (748), Expect = 2.2e-75
Identity = 143/184 (77.72%), Postives = 157/184 (85.33%), Query Frame = 0


Query: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60
           MGF D +TEGILR  AII LVL+VLLLGFDK+T QIFH+DKKASF+SL A V+I+YVDS+
Sbjct: 1   MGFGDVKTEGILRFCAIILLVLSVLLLGFDKETVQIFHVDKKASFKSLRALVIIIYVDSM 60

Query: 61  AAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120
           AA YNILQ+CKCWI AQPKG SKLG+  HI LFW SF LDQ  AYL FA+NTAGMEAALL
Sbjct: 61  AAGYNILQLCKCWIFAQPKGISKLGTHFHIYLFWLSFFLDQVAAYLTFASNTAGMEAALL 120

Query: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180
           AVTGAHDFQWMKLCNRF RFCYQVGGAFLCGY AFFA+  I  IS+FNLFRHYSPN F R
Sbjct: 121 AVTGAHDFQWMKLCNRFHRFCYQVGGAFLCGYAAFFALLLISFISAFNLFRHYSPNHFLR 180

Query: 181 LKSN 185
           LKS+
Sbjct: 181 LKSS 184

[Close]

BLAST of Carg23481 vs. NCBI nr
Match: XP_038889630.1 (CASP-like protein 2C1 [Benincasa hispida])

HSP 1 Score: 277.7 bits (709), Expect = 7.2e-71
Identity = 139/184 (75.54%), Postives = 149/184 (80.98%), Query Frame = 0


Query: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60
           MGF + RTEGILR  AI  LVL+V LLGFDKQTTQIFHIDKKASFRSL A V+I+YVDS+
Sbjct: 1   MGFGNVRTEGILRFCAIFLLVLSVCLLGFDKQTTQIFHIDKKASFRSLRALVIIIYVDSM 60

Query: 61  AAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120
            A YNILQICKCWI AQPKG SKLG    I LFW SF LDQA  YL FAANTAGMEA+ L
Sbjct: 61  VAGYNILQICKCWIFAQPKGISKLGGHFDIYLFWLSFFLDQAAVYLTFAANTAGMEASFL 120

Query: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180
           AVTGA DFQWMKLCNRF RFCYQVGGAFLCGY AF  +  I  IS+F+LFRHYSPN F R
Sbjct: 121 AVTGARDFQWMKLCNRFHRFCYQVGGAFLCGYAAFSTLLVISFISAFSLFRHYSPNHFLR 180

Query: 181 LKSN 185
           LKS+
Sbjct: 181 LKSS 184

[Close]

BLAST of Carg23481 vs. ExPASy Swiss-Prot
Match: B9IM09 (CASP-like protein 2C1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_578614 PE=3 SV=2)

HSP 1 Score: 181.4 bits (459), Expect = 9.2e-45
Identity = 92/176 (52.27%), Postives = 121/176 (68.75%), Query Frame = 0


Query: 7   RTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSLAAAYNI 66
           + E ILR  AI+ LV T  L+G D QT  +   +K+ +++ LHA VV++YVD++AAAYN+
Sbjct: 7   KIEAILRGIAILLLVSTACLVGLDSQTKFVIVYEKEVTYKDLHALVVLVYVDAVAAAYNL 66

Query: 67  LQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALLAVTGAH 126
           LQ+C+C +SA  KG  K   GS+  L WA F+LDQ  AY  FAA++A ++ ++L +TGA 
Sbjct: 67  LQLCRCSVSALSKGNFK---GSYRYLSWACFVLDQLAAYTTFAAHSAALQHSVLGITGAK 126

Query: 127 DFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFRLK 183
            FQWMK CNRF RFC+Q+GGA  CGY A   M  I  IS+FNLFR YSP  F RLK
Sbjct: 127 VFQWMKWCNRFTRFCFQIGGALTCGYIASVLMVMISFISAFNLFRLYSPKHFLRLK 179

[Close]

BLAST of Carg23481 vs. ExPASy Swiss-Prot
Match: Q5K4H9 (CASP-like protein XL3 OS=Gossypium hirsutum OX=3635 GN=XL3 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.1e-42
Identity = 89/177 (50.28%), Postives = 117/177 (66.10%), Query Frame = 0


Query: 6   QRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSLAAAYN 65
           Q+ E ++R   I+ LVLT  L+G D QT  IF++ KKASF+ L A V ++Y+ SLAAAYN
Sbjct: 6   QKIEALIRLSTIVMLVLTACLIGLDSQTKVIFYVQKKASFKDLRALVGLLYITSLAAAYN 65

Query: 66  ILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALLAVTGA 125
           +LQ+C    SA  KGTS     S+  L W  ++LDQAV Y VFA N A +E + L +TG 
Sbjct: 66  LLQLCCSSFSASYKGTS---LQSYAYLAWLRYILDQAVVYAVFAGNLAALEHSFLVLTGE 125

Query: 126 HDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFRLK 183
            +FQW+K CN++ RFC Q+GG+ LCG+ A   M +I  IS+FNLFR YSP  F  LK
Sbjct: 126 ENFQWLKWCNKYTRFCTQIGGSLLCGFVASLLMFSIASISAFNLFRQYSPTKFMHLK 179

[Close]

BLAST of Carg23481 vs. ExPASy Swiss-Prot
Match: A7Q6G6 (CASP-like protein 2C1 OS=Vitis vinifera OX=29760 GN=VIT_11s0052g01140 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.5e-42
Identity = 96/177 (54.24%), Postives = 122/177 (68.93%), Query Frame = 0


Query: 7   RTEGILRSFAIIGLVLTVLLLGFDKQTTQIFH-IDKKASFRSLHAFVVIMYVDSLAAAYN 66
           R E  LR FAI+ LVLT  LLGFD QT  +F  I K A+FR L A  V++YVDS+AA YN
Sbjct: 30  RAEAFLRLFAILVLVLTACLLGFDTQTKLLFSTIKKTATFRDLGALQVVVYVDSVAAGYN 89

Query: 67  ILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALLAVTGA 126
           +LQ+ + +ISA+ KG  KL + S++ L W  FLLDQA  Y VF+ANTA ++A+++AVTG 
Sbjct: 90  LLQLGRGFISAKLKG--KLINVSYVTLPWVCFLLDQAAVYTVFSANTAALQASIIAVTGE 149

Query: 127 HDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFRLK 183
              QWMK+CNR+ RFC QVGGA L GY A   M  +  +S+F+LFR YSP  F  LK
Sbjct: 150 SSLQWMKVCNRYTRFCIQVGGALLSGYLASLLMVLLSSLSAFSLFRLYSPKQFHLLK 204

[Close]

BLAST of Carg23481 vs. ExPASy Swiss-Prot
Match: C6SZ04 (CASP-like protein 2C1 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 6.4e-38
Identity = 84/178 (47.19%), Postives = 116/178 (65.17%), Query Frame = 0


Query: 7   RTEGILRSFAIIGLVLTVLLLGFDKQTTQIF-HIDKKASFRSLHAFVVIMYVDSLAAAYN 66
           R E  LR  AI+ LVLT  L+  D QT  +F  I+KKA+++ L+A  +++YV   AA YN
Sbjct: 7   RGEVCLRVSAILVLVLTACLVALDTQTKVVFVSIEKKATYKDLNALKILVYVTCAAAGYN 66

Query: 67  ILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALLAVTGA 126
           +L +CK  I ++     K   GS++C+ W  F LDQ   Y+ FAANTA M AA+LA++G+
Sbjct: 67  LLLLCKHSIWSR-----KNFKGSYLCMAWICFSLDQIAVYMTFAANTATMGAAVLAISGS 126

Query: 127 HDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFRLKS 184
             FQW+K+C++F RFC ++GGA LCGY A   M  I  IS++ +FR YSP  F RLKS
Sbjct: 127 EAFQWLKVCDKFTRFCVEIGGALLCGYAASMLMALISTISAYKVFRMYSPKWFLRLKS 179

[Close]

BLAST of Carg23481 vs. ExPASy Swiss-Prot
Match: Q8L8U9 (CASP-like protein 2C1 OS=Arabidopsis thaliana OX=3702 GN=At4g25830 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 8.1e-33
Identity = 77/179 (43.02%), Postives = 107/179 (59.78%), Query Frame = 0


Query: 6   QRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSLAAAYN 65
           + TE ILR   +  L+LT  L+G D QT +I +I K  SFR L A    +Y+D + AAYN
Sbjct: 5   RETEVILRLCIVFFLLLTSCLIGLDSQTKEIAYIHKNVSFRYLLALEAELYIDVVVAAYN 64

Query: 66  ILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALLAVTGA 125
           ++Q+   W + + K ++           W S+LLDQ  AY+VFA  +A  + +LL VTG+
Sbjct: 65  LVQLGLGWYNVEQKTSNPK---------WFSYLLDQTAAYVVFAGTSAAAQHSLLVVTGS 124

Query: 126 HDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFRLKSN 185
            + QWMK C +F RFC+Q+G A +  Y A   M  +  IS+FNLFR YSP  FFR KS+
Sbjct: 125 RELQWMKWCYKFTRFCFQMGSAIILNYIAAALMVLLSSISAFNLFRLYSPKRFFRFKSS 174

[Close]

BLAST of Carg23481 vs. ExPASy TrEMBL
Match: A0A6J1FCZ2 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444261 PE=3 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 4.2e-101
Identity = 184/185 (99.46%), Postives = 184/185 (99.46%), Query Frame = 0


Query: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60
           MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL
Sbjct: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60

Query: 61  AAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120
           AAAYNILQICKCWISA PKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL
Sbjct: 61  AAAYNILQICKCWISAHPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120

Query: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180
           AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR
Sbjct: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180

Query: 181 LKSNE 186
           LKSNE
Sbjct: 181 LKSNE 185

[Close]

BLAST of Carg23481 vs. ExPASy TrEMBL
Match: A0A6J1HQ91 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465689 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 4.7e-100
Identity = 182/185 (98.38%), Postives = 183/185 (98.92%), Query Frame = 0


Query: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60
           MGFWDQ+TEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL
Sbjct: 1   MGFWDQKTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60

Query: 61  AAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120
           AAAYNILQIC CWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL
Sbjct: 61  AAAYNILQICNCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120

Query: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180
           AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHY PNLFFR
Sbjct: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYLPNLFFR 180

Query: 181 LKSNE 186
           LKSNE
Sbjct: 181 LKSNE 185

[Close]

BLAST of Carg23481 vs. ExPASy TrEMBL
Match: A0A0A0LK62 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G048830 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 1.0e-75
Identity = 143/184 (77.72%), Postives = 157/184 (85.33%), Query Frame = 0


Query: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60
           MGF D +TEGILR  AII LVL+VLLLGFDK+T QIFH+DKKASF+SL A V+I+YVDS+
Sbjct: 1   MGFGDVKTEGILRFCAIILLVLSVLLLGFDKETVQIFHVDKKASFKSLRALVIIIYVDSM 60

Query: 61  AAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120
           AA YNILQ+CKCWI AQPKG SKLG+  HI LFW SF LDQ  AYL FA+NTAGMEAALL
Sbjct: 61  AAGYNILQLCKCWIFAQPKGISKLGTHFHIYLFWLSFFLDQVAAYLTFASNTAGMEAALL 120

Query: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180
           AVTGAHDFQWMKLCNRF RFCYQVGGAFLCGY AFFA+  I  IS+FNLFRHYSPN F R
Sbjct: 121 AVTGAHDFQWMKLCNRFHRFCYQVGGAFLCGYAAFFALLLISFISAFNLFRHYSPNHFLR 180

Query: 181 LKSN 185
           LKS+
Sbjct: 181 LKSS 184

[Close]

BLAST of Carg23481 vs. ExPASy TrEMBL
Match: A0A6J1DH36 (CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111020824 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.2e-68
Identity = 133/185 (71.89%), Postives = 155/185 (83.78%), Query Frame = 0


Query: 1   MGFW-DQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDS 60
           MGF+ D RTEGILR  AIIGL+LTV LLGFDKQTTQIFH+DKKA+FRSL + +VIMYV+S
Sbjct: 1   MGFFGDPRTEGILRFCAIIGLILTVCLLGFDKQTTQIFHVDKKATFRSLRSLIVIMYVNS 60

Query: 61  LAAAYNILQICKCWISAQPK-GTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAA 120
           +AA YN+LQ+CKCWISAQPK G SKLG+   I +FW SF LDQA+AY++FAANTAG+EAA
Sbjct: 61  VAAGYNLLQLCKCWISAQPKLGASKLGAHYDIYIFWISFFLDQAIAYVIFAANTAGVEAA 120

Query: 121 LLAVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLF 180
           LLA+TGA++ QWMKLCNRF RFC+Q GGAFLCGY A   M AI  IS+FNLF HYSP+ F
Sbjct: 121 LLAITGAYNLQWMKLCNRFTRFCFQAGGAFLCGYAASLTMAAISFISAFNLFTHYSPSHF 180

Query: 181 FRLKS 184
            R KS
Sbjct: 181 LRPKS 185

[Close]

BLAST of Carg23481 vs. ExPASy TrEMBL
Match: A0A6J1KBE1 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111492374 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.7e-63
Identity = 126/184 (68.48%), Postives = 142/184 (77.17%), Query Frame = 0


Query: 1   MGFWDQRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSL 60
           MGF D RTEGILR  A+I LV TV L+GFDK+TTQIF  DK ASF+SL A  VIMYVDS+
Sbjct: 1   MGFGDARTEGILRLCAMICLVFTVFLMGFDKETTQIFGADKTASFKSLGALKVIMYVDSI 60

Query: 61  AAAYNILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALL 120
           AAAYNILQ+ KCWISA PK   +L +  H+ LFW SFL+DQA AYL FAANTAG+EAA +
Sbjct: 61  AAAYNILQLSKCWISAHPKPPFQLQTHFHLPLFWLSFLIDQAGAYLTFAANTAGIEAAFV 120

Query: 121 AVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFR 180
           AV G  +FQWMKLCNRF RFCYQ  GAFLCGY AF  M  I  +S+FNLF+HYSPN F  
Sbjct: 121 AVRGNQEFQWMKLCNRFHRFCYQAAGAFLCGYAAFLTMLLISFLSAFNLFKHYSPNHFLH 180

Query: 181 LKSN 185
           LKSN
Sbjct: 181 LKSN 184

[Close]

BLAST of Carg23481 vs. TAIR 10
Match: AT4G25830.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 141.7 bits (356), Expect = 5.8e-34
Identity = 77/179 (43.02%), Postives = 107/179 (59.78%), Query Frame = 0


Query: 6   QRTEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSLAAAYN 65
           + TE ILR   +  L+LT  L+G D QT +I +I K  SFR L A    +Y+D + AAYN
Sbjct: 5   RETEVILRLCIVFFLLLTSCLIGLDSQTKEIAYIHKNVSFRYLLALEAELYIDVVVAAYN 64

Query: 66  ILQICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALLAVTGA 125
           ++Q+   W + + K ++           W S+LLDQ  AY+VFA  +A  + +LL VTG+
Sbjct: 65  LVQLGLGWYNVEQKTSNPK---------WFSYLLDQTAAYVVFAGTSAAAQHSLLVVTGS 124

Query: 126 HDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHYSPNLFFRLKSN 185
            + QWMK C +F RFC+Q+G A +  Y A   M  +  IS+FNLFR YSP  FFR KS+
Sbjct: 125 RELQWMKWCYKFTRFCFQMGSAIILNYIAAALMVLLSSISAFNLFRLYSPKRFFRFKSS 174

[Close]

BLAST of Carg23481 vs. TAIR 10
Match: AT2G35760.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 100.5 bits (249), Expect = 1.5e-21
Identity = 61/166 (36.75%), Postives = 86/166 (51.81%), Query Frame = 0


Query: 8   TEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSLAAAYNIL 67
           TE ILR    +  ++  +L+  D Q  +IF I KKA F  + A V+++ V+ +AA Y+++
Sbjct: 30  TELILRCLVCVLALVAAILIATDVQVREIFMIQKKAKFTDMKALVLLVVVNGIAAGYSLV 89

Query: 68  QICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALLAVTGAHD 127
           Q  +C +    KG           L WA F  DQAVAYL  A   A  ++A  A  G  +
Sbjct: 90  QAVRCVVGLM-KGRVLFSKP----LAWAIFFGDQAVAYLCVAGVAAAAQSAAFAKLGEPE 149

Query: 128 FQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHY 174
            QWMK+CN + +FC QVG        A   M  I  IS+F +FR Y
Sbjct: 150 LQWMKICNMYGKFCNQVGEGIASALFACIGMVLISCISAFGVFRLY 190

[Close]

BLAST of Carg23481 vs. TAIR 10
Match: AT4G16442.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 85.5 bits (210), Expect = 4.9e-17
Identity = 57/166 (34.34%), Postives = 86/166 (51.81%), Query Frame = 0


Query: 8   TEGILRSFAIIGLVLTVLLLGFDKQTTQIFHIDKKASFRSLHAFVVIMYVDSLAAAYNIL 67
           TE +LR    +  +L ++L+  D +   IF I K A +  + A V ++  + +AA Y++L
Sbjct: 11  TELLLRCSISVFALLALILVVTDTEVKLIFTIKKTAKYTDMKAVVFLVVANGIAAVYSLL 70

Query: 68  QICKCWISAQPKGTSKLGSGSHICLFWASFLLDQAVAYLVFAANTAGMEAALLAVTGAHD 127
           Q  +C +     GT K        L WA F  DQA+AYL  AA  A  E+ ++A  G  D
Sbjct: 71  QSVRCVV-----GTMKGKVLFSKPLAWAFFSGDQAMAYLNVAAIAATAESGVIAREGEED 130

Query: 128 FQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFRHY 174
            QWM++C  + +FC Q+         A  AM  +  IS+F+LFR Y
Sbjct: 131 LQWMRVCTMYGKFCNQMAIGVSSALLASIAMVFVSCISAFSLFRLY 171

[Close]

BLAST of Carg23481 vs. TAIR 10
Match: AT4G15610.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 46.2 bits (108), Expect = 3.3e-05
Identity = 42/172 (24.42%), Postives = 76/172 (44.19%), Query Frame = 0


Query: 8   TEGILRSFAIIGLVLTVLLLGFDKQTTQIFHID-----KKASFRSLHAFVVIMYVDSLAA 67
           T+ +LR       + +++++   KQT  IF          A F +  A +  +   S+A 
Sbjct: 29  TQVVLRFVLFAATLTSIVVMVTSKQTKNIFLPGTPIRIPAAEFTNSPALIYFVVALSVAC 88

Query: 68  AYNILQICKCWISAQPKGTSKLGSGSHIC---LFWASFLLDQAVAYLVFAANTAGMEAAL 127
            Y+I+      +SA  K         H C   L     ++D  +  +V +A  AG   A 
Sbjct: 89  FYSIVSTF-VTVSAFKK---------HSCSAVLLLNLAIMDAVMVGIVASATGAGGGVAY 148

Query: 128 LAVTGAHDFQWMKLCNRFERFCYQVGGAFLCGYGAFFAMCAICVISSFNLFR 172
           L + G  + +W K+C+ +++FC  VGGA      A   +  + +IS  +L++
Sbjct: 149 LGLKGNKEVRWGKICHIYDKFCRHVGGAIAVSLFASVVLLLLSIISVLSLYK 190

The following BLAST results are available for this feature:

BLAST of Carg23481 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023537147.1	2.3e-101	100.00	CASP-like protein 2C1 [Cucurbita pepo subsp. pepo] >KAG6586191.1 CASP-like prote...	[more]
XP_022938059.1	8.7e-101	99.46	CASP-like protein 2C1 [Cucurbita moschata]	[more]
XP_022965970.1	9.7e-100	98.38	CASP-like protein 2C1 [Cucurbita maxima]	[more]
XP_004149074.1	2.2e-75	77.72	CASP-like protein 2C1 [Cucumis sativus] >KGN61092.1 hypothetical protein Csa_021...	[more]
XP_038889630.1	7.2e-71	75.54	CASP-like protein 2C1 [Benincasa hispida]	[more]

BLAST of Carg23481 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9IM09	9.2e-45	52.27	CASP-like protein 2C1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_578614 PE=3 S...	[more]
Q5K4H9	1.1e-42	50.28	CASP-like protein XL3 OS=Gossypium hirsutum OX=3635 GN=XL3 PE=2 SV=1	[more]
A7Q6G6	2.5e-42	54.24	CASP-like protein 2C1 OS=Vitis vinifera OX=29760 GN=VIT_11s0052g01140 PE=2 SV=1	[more]
C6SZ04	6.4e-38	47.19	CASP-like protein 2C1 OS=Glycine max OX=3847 PE=2 SV=1	[more]
Q8L8U9	8.1e-33	43.02	CASP-like protein 2C1 OS=Arabidopsis thaliana OX=3702 GN=At4g25830 PE=2 SV=1	[more]

BLAST of Carg23481 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FCZ2	4.2e-101	99.46	CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444261 PE=3 SV=1	[more]
A0A6J1HQ91	4.7e-100	98.38	CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465689 PE=3 SV=1	[more]
A0A0A0LK62	1.0e-75	77.72	CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G048830 PE=3 SV=1	[more]
A0A6J1DH36	1.2e-68	71.89	CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111020824 PE=3 SV=1	[more]
A0A6J1KBE1	2.7e-63	68.48	CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111492374 PE=3 SV=1	[more]

BLAST of Carg23481 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25830.1	5.8e-34	43.02	Uncharacterised protein family (UPF0497)	[more]
AT2G35760.1	1.5e-21	36.75	Uncharacterised protein family (UPF0497)	[more]
AT4G16442.1	4.9e-17	34.34	Uncharacterised protein family (UPF0497)	[more]
AT4G15610.1	3.3e-05	24.42	Uncharacterised protein family (UPF0497)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006702	Casparian strip membrane protein domain	PFAM	PF04535	DUF588	coord: 8..157 e-value: 1.9E-31 score: 108.9
IPR006459	Casparian strip membrane protein	TIGRFAM	TIGR01569	TIGR01569	coord: 11..168 e-value: 3.4E-23 score: 80.2
None	No IPR available	PANTHER	PTHR11615	NITRATE, FORMATE, IRON DEHYDROGENASE	coord: 4..183
None	No IPR available	PANTHER	PTHR11615:SF241	CASP-LIKE PROTEIN 2C1	coord: 4..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23481-RA	Carg23481-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding