Carg23446 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23446
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Calmodulin-like
Location	Carg_Chr05: 10774957 .. 10776888 (-)
RNA-Seq Expression	Carg23446
Synteny	Carg23446

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGAAGAGCAAATTTTAGAGTTTCAAGAAGCTTTTTGTCTGCTCGACAAAGATGGTGATGGTACGTGTGAGTTATGAATATCGTAATCAAATTCTCTTCGATCACCGCTCGTGGCAGGTGACCGTTGCCACGATCGACCTTTTGTTCTTGAATTTTTGAATTACCGATCATCGTTTGATAACCATTTTGTTGTTCGAGTTTTTCGTTTTTTTGTAAAATAATTGTATGATCTATTCTCATAATTTCTTTAGGTCGAACTTTTTTTTTCGTTTTATGATGAAAACTCGAATTTCTGAGGCTAATGATATACGTCATAGCCCGTTGAGTTACGTTCAAGATGACTAGATATCTAATCGTTATTATGAACGTTTTTTGAAATATAGGTTGCATAACGATCAAAGAACTTGCTACAGCGATTAGATCGTTACATCAAAACCCTACTGAAGAAGAACTGCAAATCATGATGAATGAAGTTGATGTTAACGGGAGCGGATCCATTGAATTTGAGGAGTTCTTCAATCTCATGGCAAAAAAGATGAAGGTATTTATTAACGTTAATTTCAATTATACGCTTAAACTTTATAAAATTTTCATTTATAATAATAAAAAATTGACGTACCATCTAATATTTAAAAATTAAAAGGCGTATATGATATTATTTATAACTATATAGATTATTGATACAATTTATTTTAAAATATTATTATCATTTAATATACATTAATAGTCACGTAATTACGTAAATAATATAGTTATTTTTAGTAAATTTCAAAGGTAAAAAAAAAAAAATAATAAAAAAAAAAAAAAAAAAAAGTCTAAGGGCATAAATGTAAACTTACTTCCAAATTCGGGTATTTTAATTATTTATTTATTTTTCCACAAGGATTTTTCAAATAATCAATTATATACTTTTAATTTCCACAAATTTTCAACGAGGCCAACTTGTTGGAATATGAGTTTTGACCTTTTATTTTATTTTATTTTATATTTTCGAGAATTATGTAATTTTTAATTTGTTATTTAAAGAAAGTAATTAACAGATAAATTGTTAAACAAGCCTGCTAATGTTTTTTTAAAATTATTATTAAATCTTAATTTTGTTGATAAATCAAGTCAATTACTTGTTAAAAAAGCATGTTTATGCTTAAGAAATTGATAAATAAATTAGTTTATTCAATATTTTAAAAGAAATATATTTCAATAATTTATAAAATAATTAAATAACTGTGACGTTTCGAGATAGTTTTTTTTTTTTTTAATTAGTAAAATAATAACAATAATTTTTCTTCCAAAAGAGACACAATGTGCTAACGTTCATAGAAGAAAGACTAATAAAGGGTATTTAAGGATTTATTGAAAGTTCGAGAACTAAATTTAAATATTTGAAATTTAGGAAAATGAAGCCGAAGAAGAATTGAGAGAAGCTTTCAAAGTATTTGATATGGATCAAGATGGGTTTATATCGCCCAACGAGGTTTGACTTCTAATTACCCTAAAGAAAAAGAAAAGGTTGGAAACGTGTTTTTGGTTGGTTTTTTAAAATCGATATGGTCTGATGGGTCAGTCGGTTTGAGCGTTTATGAATTCACCAGCCAACTGATTCCAATTGACACGTGAACTTAAATCGACCAACTGAAGTCAATTCGATTGATCAGCTCGATTTTCGAGTTTTGTTACTTATTTCTACTTAAAACATTTATGAATCCCTATCGATCATTTTCGATCCCTAAACCCTAACTCGAAAAACCCGAGAAAAAAGGGTTAAAAGTGGTTTAATTTTGTGTGTTTGGCAGCTGAAGAATGTGATGATCCACATGGTGGAGAAATTAACTGATGAAGAAGTAGAGCAAATGGTGAAAGAAGCTGATTTGGATGGAGATGGTCTTATTGACTATGAAGAATTTGCTAAAATGATGCTTCTTATTTGA

mRNA sequence

ATGTCGGAAGAGCAAATTTTAGAGTTTCAAGAAGCTTTTTGTCTGCTCGACAAAGATGGTGATGGTTGCATAACGATCAAAGAACTTGCTACAGCGATTAGATCGTTACATCAAAACCCTACTGAAGAAGAACTGCAAATCATGATGAATGAAGTTGATGTTAACGGGAGCGGATCCATTGAATTTGAGGAGTTCTTCAATCTCATGGCAAAAAAGATGAAGGAAAATGAAGCCGAAGAAGAATTGAGAGAAGCTTTCAAAGTATTTGATATGGATCAAGATGGGTTTATATCGCCCAACGAGCTGAAGAATGTGATGATCCACATGGTGGAGAAATTAACTGATGAAGAAGTAGAGCAAATGGTGAAAGAAGCTGATTTGGATGGAGATGGTCTTATTGACTATGAAGAATTTGCTAAAATGATGCTTCTTATTTGA

Coding sequence (CDS)

ATGTCGGAAGAGCAAATTTTAGAGTTTCAAGAAGCTTTTTGTCTGCTCGACAAAGATGGTGATGGTTGCATAACGATCAAAGAACTTGCTACAGCGATTAGATCGTTACATCAAAACCCTACTGAAGAAGAACTGCAAATCATGATGAATGAAGTTGATGTTAACGGGAGCGGATCCATTGAATTTGAGGAGTTCTTCAATCTCATGGCAAAAAAGATGAAGGAAAATGAAGCCGAAGAAGAATTGAGAGAAGCTTTCAAAGTATTTGATATGGATCAAGATGGGTTTATATCGCCCAACGAGCTGAAGAATGTGATGATCCACATGGTGGAGAAATTAACTGATGAAGAAGTAGAGCAAATGGTGAAAGAAGCTGATTTGGATGGAGATGGTCTTATTGACTATGAAGAATTTGCTAAAATGATGCTTCTTATTTGA

Protein sequence

MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSIEFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQMVKEADLDGDGLIDYEEFAKMMLLI

Homology

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BLAST of Carg23446 vs. NCBI nr
Match: KAG7030302.1 (Calmodulin-like protein 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 282.7 bits (722), Expect = 1.8e-72
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI
Sbjct: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ
Sbjct: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120

Query: 121 MVKEADLDGDGLIDYEEFAKMMLLI 146
           MVKEADLDGDGLIDYEEFAKMMLLI
Sbjct: 121 MVKEADLDGDGLIDYEEFAKMMLLI 145

[Close]

BLAST of Carg23446 vs. NCBI nr
Match: XP_023547072.1 (calmodulin-like protein 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 280.8 bits (717), Expect = 6.7e-72
Identity = 144/145 (99.31%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI
Sbjct: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           EFEEFFNLMAKKMKE+EAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ
Sbjct: 61  EFEEFFNLMAKKMKESEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120

Query: 121 MVKEADLDGDGLIDYEEFAKMMLLI 146
           MVKEADLDGDGLIDYEEFAKMMLLI
Sbjct: 121 MVKEADLDGDGLIDYEEFAKMMLLI 145

[Close]

BLAST of Carg23446 vs. NCBI nr
Match: XP_022946612.1 (calmodulin-like protein 8 [Cucurbita moschata])

HSP 1 Score: 280.8 bits (717), Expect = 6.7e-72
Identity = 144/145 (99.31%), Postives = 144/145 (99.31%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI
Sbjct: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           EFEEFFNLM KKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ
Sbjct: 61  EFEEFFNLMEKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120

Query: 121 MVKEADLDGDGLIDYEEFAKMMLLI 146
           MVKEADLDGDGLIDYEEFAKMMLLI
Sbjct: 121 MVKEADLDGDGLIDYEEFAKMMLLI 145

[Close]

BLAST of Carg23446 vs. NCBI nr
Match: XP_022999410.1 (calmodulin-like protein 8 [Cucurbita maxima])

HSP 1 Score: 279.3 bits (713), Expect = 1.9e-71
Identity = 142/145 (97.93%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQ+MMNEVD+NGSGSI
Sbjct: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQMMMNEVDINGSGSI 60

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELK+VMIHMVEKLTDEEVEQ
Sbjct: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKHVMIHMVEKLTDEEVEQ 120

Query: 121 MVKEADLDGDGLIDYEEFAKMMLLI 146
           MVKEADLDGDGLIDYEEFAKMMLLI
Sbjct: 121 MVKEADLDGDGLIDYEEFAKMMLLI 145

[Close]

BLAST of Carg23446 vs. NCBI nr
Match: KAG6599302.1 (Calmodulin-like protein 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 277.3 bits (708), Expect = 7.4e-71
Identity = 142/145 (97.93%), Postives = 144/145 (99.31%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           MSEEQILEF+EAFCLLDKDGDGCITIKELATAIRSL QNPTEEELQIMMNEVDVNGSGSI
Sbjct: 1   MSEEQILEFEEAFCLLDKDGDGCITIKELATAIRSLQQNPTEEELQIMMNEVDVNGSGSI 60

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTD+EVEQ
Sbjct: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDDEVEQ 120

Query: 121 MVKEADLDGDGLIDYEEFAKMMLLI 146
           MVKEADLDGDGLIDYEEFAKMMLLI
Sbjct: 121 MVKEADLDGDGLIDYEEFAKMMLLI 145

[Close]

BLAST of Carg23446 vs. ExPASy Swiss-Prot
Match: Q9LIK5 (Calmodulin-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=CML11 PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 4.0e-51
Identity = 95/144 (65.97%), Postives = 127/144 (88.19%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           +++EQI+EF+EAFCL DKDGDGCIT  ELAT IRSL QNPTE+ELQ M+ E+D +G+G+I
Sbjct: 28  LTQEQIMEFKEAFCLFDKDGDGCITADELATVIRSLDQNPTEQELQDMITEIDSDGNGTI 87

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           EF EF NLMA +++E +A+EEL+EAFKVFD DQ+G+IS +EL++VMI++ EKLTDEEV+Q
Sbjct: 88  EFSEFLNLMANQLQETDADEELKEAFKVFDKDQNGYISASELRHVMINLGEKLTDEEVDQ 147

Query: 121 MVKEADLDGDGLIDYEEFAKMMLL 145
           M+KEADLDGDG ++Y+EF +MM++
Sbjct: 148 MIKEADLDGDGQVNYDEFVRMMMI 171

[Close]

BLAST of Carg23446 vs. ExPASy Swiss-Prot
Match: O23320 (Calmodulin-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=CML8 PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 5.2e-51
Identity = 97/145 (66.90%), Postives = 127/145 (87.59%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           ++++QI EF+EAFCL DKDGDGCIT++ELAT IRSL QNPTE+EL  ++ E+D + +G+I
Sbjct: 6   LTKDQITEFKEAFCLFDKDGDGCITVEELATVIRSLDQNPTEQELHDIITEIDSDSNGTI 65

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           EF EF NLMAKK++E++AEEEL+EAFKVFD DQ+G+IS +EL +VMI++ EKLTDEEVEQ
Sbjct: 66  EFAEFLNLMAKKLQESDAEEELKEAFKVFDKDQNGYISASELSHVMINLGEKLTDEEVEQ 125

Query: 121 MVKEADLDGDGLIDYEEFAKMMLLI 146
           M+KEADLDGDG ++Y+EF KMM+ I
Sbjct: 126 MIKEADLDGDGQVNYDEFVKMMINI 150

[Close]

BLAST of Carg23446 vs. ExPASy Swiss-Prot
Match: P0DH95 (Calmodulin-1 OS=Arabidopsis thaliana OX=3702 GN=CAM1 PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 1.7e-49
Identity = 96/143 (67.13%), Postives = 123/143 (86.01%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           +++EQI EF+EAF L DKDGDGCIT KEL T +RSL QNPTE ELQ M+NEVD +G+G+I
Sbjct: 5   LTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 64

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           +F EF NLMAKKMK+ ++EEEL+EAF+VFD DQ+GFIS  EL++VM ++ EKLTDEEVE+
Sbjct: 65  DFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEE 124

Query: 121 MVKEADLDGDGLIDYEEFAKMML 144
           M++EAD+DGDG I+YEEF K+M+
Sbjct: 125 MIREADVDGDGQINYEEFVKIMM 147

[Close]

BLAST of Carg23446 vs. ExPASy Swiss-Prot
Match: P0DH96 (Calmodulin-4 OS=Arabidopsis thaliana OX=3702 GN=CAM4 PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 1.7e-49
Identity = 96/143 (67.13%), Postives = 123/143 (86.01%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           +++EQI EF+EAF L DKDGDGCIT KEL T +RSL QNPTE ELQ M+NEVD +G+G+I
Sbjct: 5   LTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 64

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           +F EF NLMAKKMK+ ++EEEL+EAF+VFD DQ+GFIS  EL++VM ++ EKLTDEEVE+
Sbjct: 65  DFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEE 124

Query: 121 MVKEADLDGDGLIDYEEFAKMML 144
           M++EAD+DGDG I+YEEF K+M+
Sbjct: 125 MIREADVDGDGQINYEEFVKIMM 147

[Close]

BLAST of Carg23446 vs. ExPASy Swiss-Prot
Match: P04464 (Calmodulin OS=Triticum aestivum OX=4565 PE=1 SV=3)

HSP 1 Score: 196.8 bits (499), Expect = 1.7e-49
Identity = 95/143 (66.43%), Postives = 123/143 (86.01%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           +++EQI EF+EAF L DKDGDGCIT KEL T +RSL QNPTE ELQ M+NEVD +G+G+I
Sbjct: 5   LTDEQIAEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 64

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           +F EF NLMA+KMK+ ++EEEL+EAF+VFD DQDGFIS  EL++VM ++ EKLTDEEV++
Sbjct: 65  DFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQDGFISAAELRHVMTNLGEKLTDEEVDE 124

Query: 121 MVKEADLDGDGLIDYEEFAKMML 144
           M++EAD+DGDG I+YEEF K+M+
Sbjct: 125 MIREADVDGDGQINYEEFVKVMM 147

[Close]

BLAST of Carg23446 vs. ExPASy TrEMBL
Match: A0A6J1G467 (calmodulin-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111450628 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 3.2e-72
Identity = 144/145 (99.31%), Postives = 144/145 (99.31%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI
Sbjct: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           EFEEFFNLM KKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ
Sbjct: 61  EFEEFFNLMEKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120

Query: 121 MVKEADLDGDGLIDYEEFAKMMLLI 146
           MVKEADLDGDGLIDYEEFAKMMLLI
Sbjct: 121 MVKEADLDGDGLIDYEEFAKMMLLI 145

[Close]

BLAST of Carg23446 vs. ExPASy TrEMBL
Match: A0A6J1KFB0 (calmodulin-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493791 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 9.4e-72
Identity = 142/145 (97.93%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQ+MMNEVD+NGSGSI
Sbjct: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQMMMNEVDINGSGSI 60

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELK+VMIHMVEKLTDEEVEQ
Sbjct: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKHVMIHMVEKLTDEEVEQ 120

Query: 121 MVKEADLDGDGLIDYEEFAKMMLLI 146
           MVKEADLDGDGLIDYEEFAKMMLLI
Sbjct: 121 MVKEADLDGDGLIDYEEFAKMMLLI 145

[Close]

BLAST of Carg23446 vs. ExPASy TrEMBL
Match: A0A1S3C386 (calmodulin-like protein 8 OS=Cucumis melo OX=3656 GN=LOC103495973 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.9e-65
Identity = 134/146 (91.78%), Postives = 138/146 (94.52%), Query Frame = 0


Query: 1   MSEEQIL-EFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGS 60
           MSEEQI+ EFQEAFCLLDKDGDGCITI+ELATAIRSLH NPTEEELQIMMNEVDVNG+G 
Sbjct: 1   MSEEQIISEFQEAFCLLDKDGDGCITIEELATAIRSLHHNPTEEELQIMMNEVDVNGNGY 60

Query: 61  IEFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVE 120
           IEF EFFNLMAKKMKENEAEEELREAFKVFDMD DG ISPNELKNVMIHMVEKLTDEE+E
Sbjct: 61  IEFGEFFNLMAKKMKENEAEEELREAFKVFDMDDDGKISPNELKNVMIHMVEKLTDEEIE 120

Query: 121 QMVKEADLDGDGLIDYEEFAKMMLLI 146
           QMV EADLDGDGLIDYEEF KMMLLI
Sbjct: 121 QMVYEADLDGDGLIDYEEFVKMMLLI 146

[Close]

BLAST of Carg23446 vs. ExPASy TrEMBL
Match: A0A5D3D4E4 (Calmodulin-like protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001380 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.8e-65
Identity = 133/146 (91.10%), Postives = 138/146 (94.52%), Query Frame = 0


Query: 1   MSEEQIL-EFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGS 60
           MSEEQI+ EFQEAFCLLDKDGDGCITI+ELATAIRSLH NPTEEELQIMMNEVDVNG+G 
Sbjct: 1   MSEEQIISEFQEAFCLLDKDGDGCITIEELATAIRSLHHNPTEEELQIMMNEVDVNGNGY 60

Query: 61  IEFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVE 120
           IEF EFFNLMAKKMKENEAEEELREAFKVFDMD DG ISPNELKNVMIHMVEKLTDEE+E
Sbjct: 61  IEFGEFFNLMAKKMKENEAEEELREAFKVFDMDDDGKISPNELKNVMIHMVEKLTDEEIE 120

Query: 121 QMVKEADLDGDGLIDYEEFAKMMLLI 146
           QMV EADLDGDGL+DYEEF KMMLLI
Sbjct: 121 QMVYEADLDGDGLVDYEEFVKMMLLI 146

[Close]

BLAST of Carg23446 vs. ExPASy TrEMBL
Match: A0A0A0LFI9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G011480 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 5.0e-65
Identity = 133/146 (91.10%), Postives = 137/146 (93.84%), Query Frame = 0


Query: 1   MSEEQIL-EFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGS 60
           MSEEQI+ EFQEAFCLLDKDGDGCITI ELATAIRSLH NPTEEELQIMMNEVDVNG+G 
Sbjct: 1   MSEEQIISEFQEAFCLLDKDGDGCITINELATAIRSLHHNPTEEELQIMMNEVDVNGNGY 60

Query: 61  IEFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVE 120
           IEF EFFNLMAKKMKENEAE+ELREAFKVFDMD DG ISPNELKNVMIHMVEKLTDEE+E
Sbjct: 61  IEFGEFFNLMAKKMKENEAEDELREAFKVFDMDDDGKISPNELKNVMIHMVEKLTDEEIE 120

Query: 121 QMVKEADLDGDGLIDYEEFAKMMLLI 146
           QMV EADLDGDGLIDYEEF KMMLLI
Sbjct: 121 QMVNEADLDGDGLIDYEEFVKMMLLI 146

[Close]

BLAST of Carg23446 vs. TAIR 10
Match: AT3G22930.1 (calmodulin-like 11 )

HSP 1 Score: 202.2 bits (513), Expect = 2.8e-52
Identity = 95/144 (65.97%), Postives = 127/144 (88.19%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           +++EQI+EF+EAFCL DKDGDGCIT  ELAT IRSL QNPTE+ELQ M+ E+D +G+G+I
Sbjct: 28  LTQEQIMEFKEAFCLFDKDGDGCITADELATVIRSLDQNPTEQELQDMITEIDSDGNGTI 87

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           EF EF NLMA +++E +A+EEL+EAFKVFD DQ+G+IS +EL++VMI++ EKLTDEEV+Q
Sbjct: 88  EFSEFLNLMANQLQETDADEELKEAFKVFDKDQNGYISASELRHVMINLGEKLTDEEVDQ 147

Query: 121 MVKEADLDGDGLIDYEEFAKMMLL 145
           M+KEADLDGDG ++Y+EF +MM++
Sbjct: 148 MIKEADLDGDGQVNYDEFVRMMMI 171

[Close]

BLAST of Carg23446 vs. TAIR 10
Match: AT4G14640.1 (calmodulin 8 )

HSP 1 Score: 201.8 bits (512), Expect = 3.7e-52
Identity = 97/145 (66.90%), Postives = 127/145 (87.59%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           ++++QI EF+EAFCL DKDGDGCIT++ELAT IRSL QNPTE+EL  ++ E+D + +G+I
Sbjct: 6   LTKDQITEFKEAFCLFDKDGDGCITVEELATVIRSLDQNPTEQELHDIITEIDSDSNGTI 65

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           EF EF NLMAKK++E++AEEEL+EAFKVFD DQ+G+IS +EL +VMI++ EKLTDEEVEQ
Sbjct: 66  EFAEFLNLMAKKLQESDAEEELKEAFKVFDKDQNGYISASELSHVMINLGEKLTDEEVEQ 125

Query: 121 MVKEADLDGDGLIDYEEFAKMMLLI 146
           M+KEADLDGDG ++Y+EF KMM+ I
Sbjct: 126 MIKEADLDGDGQVNYDEFVKMMINI 150

[Close]

BLAST of Carg23446 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 196.8 bits (499), Expect = 1.2e-50
Identity = 96/143 (67.13%), Postives = 123/143 (86.01%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           +++EQI EF+EAF L DKDGDGCIT KEL T +RSL QNPTE ELQ M+NEVD +G+G+I
Sbjct: 5   LTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 64

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           +F EF NLMAKKMK+ ++EEEL+EAF+VFD DQ+GFIS  EL++VM ++ EKLTDEEVE+
Sbjct: 65  DFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEE 124

Query: 121 MVKEADLDGDGLIDYEEFAKMML 144
           M++EAD+DGDG I+YEEF K+M+
Sbjct: 125 MIREADVDGDGQINYEEFVKIMM 147

[Close]

BLAST of Carg23446 vs. TAIR 10
Match: AT5G37780.1 (calmodulin 1 )

HSP 1 Score: 196.8 bits (499), Expect = 1.2e-50
Identity = 96/143 (67.13%), Postives = 123/143 (86.01%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           +++EQI EF+EAF L DKDGDGCIT KEL T +RSL QNPTE ELQ M+NEVD +G+G+I
Sbjct: 5   LTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 64

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           +F EF NLMAKKMK+ ++EEEL+EAF+VFD DQ+GFIS  EL++VM ++ EKLTDEEVE+
Sbjct: 65  DFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEE 124

Query: 121 MVKEADLDGDGLIDYEEFAKMML 144
           M++EAD+DGDG I+YEEF K+M+
Sbjct: 125 MIREADVDGDGQINYEEFVKIMM 147

[Close]

BLAST of Carg23446 vs. TAIR 10
Match: AT2G41110.1 (calmodulin 2 )

HSP 1 Score: 194.5 bits (493), Expect = 5.9e-50
Identity = 94/143 (65.73%), Postives = 123/143 (86.01%), Query Frame = 0


Query: 1   MSEEQILEFQEAFCLLDKDGDGCITIKELATAIRSLHQNPTEEELQIMMNEVDVNGSGSI 60
           ++++QI EF+EAF L DKDGDGCIT KEL T +RSL QNPTE ELQ M+NEVD +G+G+I
Sbjct: 5   LTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 64

Query: 61  EFEEFFNLMAKKMKENEAEEELREAFKVFDMDQDGFISPNELKNVMIHMVEKLTDEEVEQ 120
           +F EF NLMA+KMK+ ++EEEL+EAF+VFD DQ+GFIS  EL++VM ++ EKLTDEEV++
Sbjct: 65  DFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDE 124

Query: 121 MVKEADLDGDGLIDYEEFAKMML 144
           M+KEAD+DGDG I+YEEF K+M+
Sbjct: 125 MIKEADVDGDGQINYEEFVKVMM 147

The following BLAST results are available for this feature:

BLAST of Carg23446 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030302.1	1.8e-72	100.00	Calmodulin-like protein 8, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023547072.1	6.7e-72	99.31	calmodulin-like protein 8 [Cucurbita pepo subsp. pepo]	[more]
XP_022946612.1	6.7e-72	99.31	calmodulin-like protein 8 [Cucurbita moschata]	[more]
XP_022999410.1	1.9e-71	97.93	calmodulin-like protein 8 [Cucurbita maxima]	[more]
KAG6599302.1	7.4e-71	97.93	Calmodulin-like protein 8, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Carg23446 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LIK5	4.0e-51	65.97	Calmodulin-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=CML11 PE=2 SV=1	[more]
O23320	5.2e-51	66.90	Calmodulin-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=CML8 PE=1 SV=1	[more]
P0DH95	1.7e-49	67.13	Calmodulin-1 OS=Arabidopsis thaliana OX=3702 GN=CAM1 PE=1 SV=1	[more]
P0DH96	1.7e-49	67.13	Calmodulin-4 OS=Arabidopsis thaliana OX=3702 GN=CAM4 PE=1 SV=1	[more]
P04464	1.7e-49	66.43	Calmodulin OS=Triticum aestivum OX=4565 PE=1 SV=3	[more]

BLAST of Carg23446 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G467	3.2e-72	99.31	calmodulin-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111450628 PE=4 SV=...	[more]
A0A6J1KFB0	9.4e-72	97.93	calmodulin-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493791 PE=4 SV=1	[more]
A0A1S3C386	2.9e-65	91.78	calmodulin-like protein 8 OS=Cucumis melo OX=3656 GN=LOC103495973 PE=4 SV=1	[more]
A0A5D3D4E4	3.8e-65	91.10	Calmodulin-like protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A0A0LFI9	5.0e-65	91.10	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G011480 PE=4 SV=1	[more]

BLAST of Carg23446 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22930.1	2.8e-52	65.97	calmodulin-like 11	[more]
AT4G14640.1	3.7e-52	66.90	calmodulin 8	[more]
AT1G66410.1	1.2e-50	67.13	calmodulin 4	[more]
AT5G37780.1	1.2e-50	67.13	calmodulin 1	[more]
AT2G41110.1	5.9e-50	65.73	calmodulin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 117..145 e-value: 3.5E-4 score: 29.9 coord: 81..109 e-value: 5.8E-7 score: 39.1 coord: 8..36 e-value: 4.3E-6 score: 36.3 coord: 44..72 e-value: 3.3E-5 score: 33.3
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 9..69 e-value: 8.4E-15 score: 55.0 coord: 79..142 e-value: 8.0E-17 score: 61.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 4..39 score: 14.652882
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 40..75 score: 13.481276
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 77..112 score: 16.410292
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 113..145 score: 14.820255
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 85..142 e-value: 1.15863E-17 score: 69.8841
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 8..70 e-value: 1.46906E-15 score: 64.4913
None	No IPR available	GENE3D	1.10.238.10		coord: 70..144 e-value: 1.1E-29 score: 104.7
None	No IPR available	GENE3D	1.10.238.10		coord: 1..69 e-value: 2.9E-26 score: 93.4
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 1..144
None	No IPR available	PANTHER	PTHR23050:SF378	CALMODULIN	coord: 1..144
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 17..29
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 90..102
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 126..138
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 53..65
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 2..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23446-RA	Carg23446-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding