Carg23275 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23275
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	dnaJ homolog subfamily B member 8
Location	Carg_Chr12: 10011090 .. 10013630 (+)
RNA-Seq Expression	Carg23275
Synteny	Carg23275

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAAACTGAAATCTCTCGAACTTTCCTTCGTTCATATCGCGTGCAAGATATTTTCATCTCTCCCTTCGCCGTCAATTCGTCCCTCGTTATAGACCATCCGCAAATCGTTGAAGAAGCCGACGTAACGGAGCTTCCTCTCTTCGTCTTCTTGTTCTTCTCTGTTTTTGAAGCCCGCGGTGGTATTATGGTGTTCTGATCCTTTGTTTGGTGGTTGTTTTTCGAGACTCTTCGGTTTCTCTTCTTTTTGATTTCGGTTTTCGATGGATCGGGGAGGAGGATCCGACGGCGGATCTTCCTACTACGCTATCCTAGGGATTCGTAAGGATGCATCCCTATCTGAAATCCGTACTGCCTATCGCAAGCTTGCTCTGGTACTGAATTCGATTCTCGATGTATTGGTTTTGAAATTCGAGTTTCTTGTTTATTCTGTTTGAGATTTTGAGTTTGGAGGAGATTTGTTTTGTTTTTTTCGATTGGAGAGTGAGTTTGTGAAATCTGAATGCGTTGTTGTTCTCGTTTGTTTTGCCGTCAAGAAATGGCATCCGGATCGGTGTACGAGGAATCCTGCTGTCGCTGGAGAAGCCAAGCGGCAGTTTCAATTAGTTCAAGAAGCGTATTCAGGTGAGATCATCGGTTCATCCGCCCCTAATCCCTGTCAATTTTGTAAACGCCGTTGAATTCCCGGTCAATGTTCATAGGAAAGGGCACTAACGCGCGTTAGATTGCTCCATCTGCCTCCTCTCTCACAGTTCTCTCCGATCAGGCGAAGAGGTCCGTTTACGACGCCGGTCTTTACGATCCGACGGAAGAAGACGATGAGGTCAGTATTGTTCTTGTTTTTTTTTTTTTTTAATTATTATTAGTATTTTCTTGGGCTCTGGTTTAGATATTTTAGCACGCTATGATTATACACTCGAAAATACGACGTCGTTTTTATCATGTTATTGGTATTATTTTTGGTAACACCTTTAAACAAATAAATAAATAAATAAAAATAAATAAAACTATATCTTTTAAAATTTTCCTTAATAATCTTAACAATAAACCTTCCTTTTTTTTTTCTTCCTTCCAAAAAATAATAATAATAATAATAAATAATGATAAAATTTCTGTGGAATTATTTTATTTCCAAAATTAATTATACGTTTCAAAAACATGCAGCAAATAAGAATTTTATCGTATCAAAATCTTAAGATTTTAAATATTTTAAATTTTAAATTACTTTATAATAAGTTTAAGTACTTACCTGAAACAATCGTATGAAAATTTATAAACAAAAAAAAATTGTTGCTGTTATTTTTTTTTAATTATTTATTTTTTTTTAATGAGATAATCTGTCATGGGACAGTATTATTTTTTCTTCATTTTTAAAATTATTTTTTTTAATAAAAATCAATACATAAATAATAAAATTTTAAAAAATAATGAAAATTTCTATGATATTTTACGGAATTATTAATCATAGTAGTTTTTTTTTTTTTTTTTTGGCGTGTGGTGCAGGAATTTTGCGGTTTCATGCAAGAGATGATATCAATGATGAACAACGTCAAACCGGAGGTAAAATCCCGACCGTTCAATTTCCTGGCAATTAATTTCTGCCATCCTTGACCGTTCATCTCCTCTATTTGAACATTATCAATAATAATAATAATAATAATAATAAATCTCTGAGAGAACCTGCTAATTAAGGTAAAATATTGCTGAATTTTATGATAAATTTTGATTTTGTCCCATTTAAATCTTTTATGTTTTATTTTTTAATTTTGAAATTGAATTAAATAAACGGAATATTATTGGTATTATTTATATATTAAAATTGAAGATATTTTTAGTTTATTTAAATGGAATTACTTAGTGATAAAAGGAATAAAGATAAATTCTTCCAAGCAAATTGAAAAATATTTACATTTTTAGATAATTGCAATATTTTAAAACAAATTTTAAAGTTAATAATAAAAATAATCTTTTTTTTATTTTTGGTTTTTGGGTTTTGCTAGGGTGACAGCTTCGAGGACCTACAGAGGATGTTCATGGAAATGGTTGGTTCAGATGGCATCGGAGGGTTCACCGTGAACGACAATCCAACGGCTTCAAAAAGGCCACGTGCAAACGGCTCCAGATCTAGCGAGGCCAAAAGAAGCACCATGCGGCGCTAAACCAAACGCCCGGTCACCGTAAGATCTTTTCCATTCTCTCTGTAATTTTCAAAAATGCCGAAAATCTCCTTAGCTGACGTGGAAATATGTGATTGGAGGTGGTGGCCATTGCCTATTTATCCCAGTTTAGGTTCCGAAGGAAGACCTGATAGTTCTTTTCTTCAAGTAATTTAGTGCAAGTTGGAGGGGCTTTTAGGTCTTTTCCTTGTTGGAGAATATTTGGCTTACTTTTTTCTCTTTATTGTATTAGAATTATATGATTATCTTTGATATAAAAATATAATTTTTCGATTAATAGTTTAGGTAATATGATATTAAAAAAAAAGTAACTTTATATTATTAAATTAAATTAAACAAAAGATACGACCAAAATTCTCCTGTAA

mRNA sequence

GAAAAAACTGAAATCTCTCGAACTTTCCTTCGTTCATATCGCGTGCAAGATATTTTCATCTCTCCCTTCGCCGTCAATTCGTCCCTCGTTATAGACCATCCGCAAATCGTTGAAGAAGCCGACGTAACGGAGCTTCCTCTCTTCGTCTTCTTGTTCTTCTCTGTTTTTGAAGCCCGCGGTGGTATTATGGTGTTCTGATCCTTTGTTTGGTGGTTGTTTTTCGAGACTCTTCGGTTTCTCTTCTTTTTGATTTCGGTTTTCGATGGATCGGGGAGGAGGATCCGACGGCGGATCTTCCTACTACGCTATCCTAGGGATTCGTAAGGATGCATCCCTATCTGAAATCCGTACTGCCTATCGCAAGCTTGCTCTGAAATGGCATCCGGATCGGTGTACGAGGAATCCTGCTGTCGCTGGAGAAGCCAAGCGGCAGTTTCAATTAGTTCAAGAAGCGTATTCAGTTCTCTCCGATCAGGCGAAGAGGTCCGTTTACGACGCCGGTCTTTACGATCCGACGGAAGAAGACGATGAGGAATTTTGCGGTTTCATGCAAGAGATGATATCAATGATGAACAACGTCAAACCGGAGGGTGACAGCTTCGAGGACCTACAGAGGATGTTCATGGAAATGGTTGGTTCAGATGGCATCGGAGGGTTCACCGTGAACGACAATCCAACGGCTTCAAAAAGGCCACGTGCAAACGGCTCCAGATCTAGCGAGGCCAAAAGAAGCACCATGCGGCGCTAAACCAAACGCCCGGTCACCGTAAGATCTTTTCCATTCTCTCTGTAATTTTCAAAAATGCCGAAAATCTCCTTAGCTGACGTGGAAATATGTGATTGGAGGTGGTGGCCATTGCCTATTTATCCCAGTTTAGGTTCCGAAGGAAGACCTGATAGTTCTTTTCTTCAAGTAATTTAGTGCAAGTTGGAGGGGCTTTTAGGTCTTTTCCTTGTTGGAGAATATTTGGCTTACTTTTTTCTCTTTATTGTATTAGAATTATATGATTATCTTTGATATAAAAATATAATTTTTCGATTAATAGTTTAGGTAATATGATATTAAAAAAAAAGTAACTTTATATTATTAAATTAAATTAAACAAAAGATACGACCAAAATTCTCCTGTAA

Coding sequence (CDS)

ATGGATCGGGGAGGAGGATCCGACGGCGGATCTTCCTACTACGCTATCCTAGGGATTCGTAAGGATGCATCCCTATCTGAAATCCGTACTGCCTATCGCAAGCTTGCTCTGAAATGGCATCCGGATCGGTGTACGAGGAATCCTGCTGTCGCTGGAGAAGCCAAGCGGCAGTTTCAATTAGTTCAAGAAGCGTATTCAGTTCTCTCCGATCAGGCGAAGAGGTCCGTTTACGACGCCGGTCTTTACGATCCGACGGAAGAAGACGATGAGGAATTTTGCGGTTTCATGCAAGAGATGATATCAATGATGAACAACGTCAAACCGGAGGGTGACAGCTTCGAGGACCTACAGAGGATGTTCATGGAAATGGTTGGTTCAGATGGCATCGGAGGGTTCACCGTGAACGACAATCCAACGGCTTCAAAAAGGCCACGTGCAAACGGCTCCAGATCTAGCGAGGCCAAAAGAAGCACCATGCGGCGCTAA

Protein sequence

MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQLVQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMFMEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMRR

Homology

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BLAST of Carg23275 vs. NCBI nr
Match: KAG6586232.1 (DnaJ-like subfamily B member 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021066.1 DnaJ-like subfamily B member 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 318.5 bits (815), Expect = 3.2e-83
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60
           MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL
Sbjct: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60

Query: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF 120
           VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF
Sbjct: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF 120

Query: 121 MEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMRR 162
           MEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMRR
Sbjct: 121 MEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMRR 161

[Close]

BLAST of Carg23275 vs. NCBI nr
Match: XP_023536754.1 (uncharacterized protein LOC111798035 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 310.1 bits (793), Expect = 1.1e-80
Identity = 156/160 (97.50%), Postives = 158/160 (98.75%), Query Frame = 0


Query: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60
           MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL
Sbjct: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60

Query: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF 120
           VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFC FMQEMISMMNNVKPEGDSFEDLQ+MF
Sbjct: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMISMMNNVKPEGDSFEDLQKMF 120

Query: 121 MEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMR 161
           MEMVGSD IGGFTVNDNPTA+KRPRANGSRSSEAKRSTMR
Sbjct: 121 MEMVGSDSIGGFTVNDNPTATKRPRANGSRSSEAKRSTMR 160

[Close]

BLAST of Carg23275 vs. NCBI nr
Match: XP_022937579.1 (uncharacterized protein LOC111443946 [Cucurbita moschata] >XP_022937580.1 uncharacterized protein LOC111443946 [Cucurbita moschata])

HSP 1 Score: 309.3 bits (791), Expect = 1.9e-80
Identity = 156/161 (96.89%), Postives = 158/161 (98.14%), Query Frame = 0


Query: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60
           MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL
Sbjct: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60

Query: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF 120
           VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFC FMQEMISMMNNVKPEGDSFEDLQ+MF
Sbjct: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMISMMNNVKPEGDSFEDLQKMF 120

Query: 121 MEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMRR 162
           MEM  SDGIGGFTVNDNPTASKRPRANGSRS+EAKRSTMRR
Sbjct: 121 MEMADSDGIGGFTVNDNPTASKRPRANGSRSTEAKRSTMRR 161

[Close]

BLAST of Carg23275 vs. NCBI nr
Match: XP_022965987.1 (dnaJ homolog subfamily B member 8 [Cucurbita maxima] >XP_022965988.1 dnaJ homolog subfamily B member 8 [Cucurbita maxima])

HSP 1 Score: 306.2 bits (783), Expect = 1.6e-79
Identity = 153/161 (95.03%), Postives = 159/161 (98.76%), Query Frame = 0


Query: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60
           MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYR LALKWHPDRCTRNP+VAGEAKRQFQL
Sbjct: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRMLALKWHPDRCTRNPSVAGEAKRQFQL 60

Query: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF 120
           VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFC FMQEMISMMN+VKPEGDSFEDLQ+MF
Sbjct: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMISMMNSVKPEGDSFEDLQKMF 120

Query: 121 MEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMRR 162
           MEM+GSDGIGGF+VNDNPTA+KRPRANGSRSSEAKRSTMRR
Sbjct: 121 MEMIGSDGIGGFSVNDNPTATKRPRANGSRSSEAKRSTMRR 161

[Close]

BLAST of Carg23275 vs. NCBI nr
Match: XP_004144765.1 (dnaJ homolog subfamily B member 8 [Cucumis sativus] >KGN60978.1 hypothetical protein Csa_021314 [Cucumis sativus])

HSP 1 Score: 286.2 bits (731), Expect = 1.8e-73
Identity = 144/161 (89.44%), Postives = 151/161 (93.79%), Query Frame = 0


Query: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60
           MDRGGGSDGG+SYYAILGIRKDASLS+IRTAYRKLALKWHPDR  RNPAVAGEAKRQFQL
Sbjct: 1   MDRGGGSDGGTSYYAILGIRKDASLSDIRTAYRKLALKWHPDRWMRNPAVAGEAKRQFQL 60

Query: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF 120
           VQEAYSVLSDQ KRSVYDAGLYDPTEEDDEEFC FMQEMI+MMNNVKPEGDSFEDLQ+MF
Sbjct: 61  VQEAYSVLSDQTKRSVYDAGLYDPTEEDDEEFCDFMQEMITMMNNVKPEGDSFEDLQKMF 120

Query: 121 MEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMRR 162
           MEMVGSDG+G F +NDNPTASKRPR NGSRSS  KRS+ RR
Sbjct: 121 MEMVGSDGVGMFNMNDNPTASKRPRPNGSRSSAPKRSSSRR 161

[Close]

BLAST of Carg23275 vs. ExPASy Swiss-Prot
Match: A6Q486 (Chaperone protein DnaJ OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 3.9e-15
Identity = 49/120 (40.83%), Postives = 69/120 (57.50%), Query Frame = 0


Query: 13  YYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQLVQEAYSVLSDQA 72
           YY +L + ++AS  EI+ AYRKLALK+HPDR   NP    EA+ +F+L+ EAY VLSD+ 
Sbjct: 6   YYELLEVDRNASFEEIKKAYRKLALKYHPDRNPDNP----EAEEKFKLINEAYQVLSDEE 65

Query: 73  KRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMFMEMVGSDGIGGF 132
           KR++YD   Y     +++ F GF Q+             SF+D+   F  + G    GGF
Sbjct: 66  KRALYDQ--YGKAGLENQGFSGFNQK-------------SFDDIMDFFESVFGETFGGGF 106

[Close]

BLAST of Carg23275 vs. ExPASy Swiss-Prot
Match: Q5SLW9 (Chaperone protein DnaJ 1 OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=dnaJ1 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.3e-14
Identity = 47/104 (45.19%), Postives = 66/104 (63.46%), Query Frame = 0


Query: 13  YYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQLVQEAYSVLSDQA 72
           YYAILG+ ++AS  EI+ AYR+LALK+HPD   RNP    EA+ +F+ + EAY+VLSD  
Sbjct: 4   YYAILGVSREASQEEIKKAYRRLALKYHPD---RNPG-DKEAEERFKEINEAYAVLSDPK 63

Query: 73  KRSVYDAG-LYDPTEEDDEEFCGFMQEMISMMNNVK-PEGDSFE 115
           KR+ YD G L  P    ++ F  F QE+  +  + + P G+  E
Sbjct: 64  KRAAYDRGHLEAPEYRPEDLFDLFFQEVFGVRGHRRPPRGEDLE 103

[Close]

BLAST of Carg23275 vs. ExPASy Swiss-Prot
Match: Q72GN6 (Chaperone protein DnaJ OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.3e-14
Identity = 47/104 (45.19%), Postives = 66/104 (63.46%), Query Frame = 0


Query: 13  YYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQLVQEAYSVLSDQA 72
           YYAILG+ ++AS  EI+ AYR+LALK+HPD   RNP    EA+ +F+ + EAY+VLSD  
Sbjct: 4   YYAILGVSREASQEEIKKAYRRLALKYHPD---RNPG-DKEAEERFKEINEAYAVLSDPK 63

Query: 73  KRSVYDAG-LYDPTEEDDEEFCGFMQEMISMMNNVK-PEGDSFE 115
           KR+ YD G L  P    ++ F  F QE+  +  + + P G+  E
Sbjct: 64  KRAAYDRGHLEAPEYRPEDLFDLFFQEVFGVRGHRRPPRGEDLE 103

[Close]

BLAST of Carg23275 vs. ExPASy Swiss-Prot
Match: Q5F3Z5 (DnaJ homolog subfamily B member 6 OS=Gallus gallus OX=9031 GN=DNAJB6 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 4.3e-14
Identity = 38/66 (57.58%), Postives = 48/66 (72.73%), Query Frame = 0


Query: 13 YYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQLVQEAYSVLSDQA 72
          YY +LG++K AS  +I+ AYRKLALKWHPD   +NP    EA++QF+ V EAY VLSD  
Sbjct: 4  YYEVLGVQKHASAEDIKKAYRKLALKWHPD---KNPENKEEAEQQFKQVAEAYEVLSDAK 63

Query: 73 KRSVYD 79
          KR +YD
Sbjct: 64 KRDIYD 66

[Close]

BLAST of Carg23275 vs. ExPASy Swiss-Prot
Match: Q8NHS0 (DnaJ homolog subfamily B member 8 OS=Homo sapiens OX=9606 GN=DNAJB8 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 7.3e-14
Identity = 37/68 (54.41%), Postives = 51/68 (75.00%), Query Frame = 0


Query: 11 SSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQLVQEAYSVLSD 70
          ++YY +LG++  AS  +I+ AYRKLAL+WHPD   +NP    EA+++F+LV EAY VLSD
Sbjct: 2  ANYYEVLGVQASASPEDIKKAYRKLALRWHPD---KNPDNKEEAEKKFKLVSEAYEVLSD 61

Query: 71 QAKRSVYD 79
            KRS+YD
Sbjct: 62 SKKRSLYD 66

[Close]

BLAST of Carg23275 vs. ExPASy TrEMBL
Match: A0A6J1FH54 (uncharacterized protein LOC111443946 OS=Cucurbita moschata OX=3662 GN=LOC111443946 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 9.4e-81
Identity = 156/161 (96.89%), Postives = 158/161 (98.14%), Query Frame = 0


Query: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60
           MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL
Sbjct: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60

Query: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF 120
           VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFC FMQEMISMMNNVKPEGDSFEDLQ+MF
Sbjct: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMISMMNNVKPEGDSFEDLQKMF 120

Query: 121 MEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMRR 162
           MEM  SDGIGGFTVNDNPTASKRPRANGSRS+EAKRSTMRR
Sbjct: 121 MEMADSDGIGGFTVNDNPTASKRPRANGSRSTEAKRSTMRR 161

[Close]

BLAST of Carg23275 vs. ExPASy TrEMBL
Match: A0A6J1HLS8 (dnaJ homolog subfamily B member 8 OS=Cucurbita maxima OX=3661 GN=LOC111465708 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 8.0e-80
Identity = 153/161 (95.03%), Postives = 159/161 (98.76%), Query Frame = 0


Query: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60
           MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYR LALKWHPDRCTRNP+VAGEAKRQFQL
Sbjct: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRMLALKWHPDRCTRNPSVAGEAKRQFQL 60

Query: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF 120
           VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFC FMQEMISMMN+VKPEGDSFEDLQ+MF
Sbjct: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMISMMNSVKPEGDSFEDLQKMF 120

Query: 121 MEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMRR 162
           MEM+GSDGIGGF+VNDNPTA+KRPRANGSRSSEAKRSTMRR
Sbjct: 121 MEMIGSDGIGGFSVNDNPTATKRPRANGSRSSEAKRSTMRR 161

[Close]

BLAST of Carg23275 vs. ExPASy TrEMBL
Match: A0A0A0LGH9 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033330 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 8.5e-74
Identity = 144/161 (89.44%), Postives = 151/161 (93.79%), Query Frame = 0


Query: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60
           MDRGGGSDGG+SYYAILGIRKDASLS+IRTAYRKLALKWHPDR  RNPAVAGEAKRQFQL
Sbjct: 1   MDRGGGSDGGTSYYAILGIRKDASLSDIRTAYRKLALKWHPDRWMRNPAVAGEAKRQFQL 60

Query: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF 120
           VQEAYSVLSDQ KRSVYDAGLYDPTEEDDEEFC FMQEMI+MMNNVKPEGDSFEDLQ+MF
Sbjct: 61  VQEAYSVLSDQTKRSVYDAGLYDPTEEDDEEFCDFMQEMITMMNNVKPEGDSFEDLQKMF 120

Query: 121 MEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMRR 162
           MEMVGSDG+G F +NDNPTASKRPR NGSRSS  KRS+ RR
Sbjct: 121 MEMVGSDGVGMFNMNDNPTASKRPRPNGSRSSAPKRSSSRR 161

[Close]

BLAST of Carg23275 vs. ExPASy TrEMBL
Match: A0A6J1G400 (dnaJ homolog subfamily B member 8-like OS=Cucurbita moschata OX=3662 GN=LOC111450533 PE=4 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.2e-73
Identity = 142/161 (88.20%), Postives = 150/161 (93.17%), Query Frame = 0


Query: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60
           MDRGGGSDGG+ YY ILGIRKDAS S++RTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL
Sbjct: 1   MDRGGGSDGGACYYTILGIRKDASFSDVRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60

Query: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF 120
           VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFC FMQEM++MMNN KPEGDSFEDLQRMF
Sbjct: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCDFMQEMMTMMNNAKPEGDSFEDLQRMF 120

Query: 121 MEMVGSDGIGGFTVNDNPTASKRPR-ANGSRSSEAKRSTMR 161
           MEMVGSDG+GGF VNDNPTASKRPR  NGSRSS  KR++ R
Sbjct: 121 MEMVGSDGMGGFQVNDNPTASKRPRPTNGSRSSAPKRNSSR 161

[Close]

BLAST of Carg23275 vs. ExPASy TrEMBL
Match: A0A5D3BDE0 (DnaJ-like protein subfamily B member 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001310 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 4.2e-73
Identity = 143/161 (88.82%), Postives = 149/161 (92.55%), Query Frame = 0


Query: 1   MDRGGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQL 60
           MDRGGGSDGG+ YY ILGIRKDASLS+IRTAYRKLALKWHPDR  RNPAVAGEAKRQFQL
Sbjct: 1   MDRGGGSDGGACYYTILGIRKDASLSDIRTAYRKLALKWHPDRWMRNPAVAGEAKRQFQL 60

Query: 61  VQEAYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMF 120
           VQEAYSVLSDQ KRSVYDAGLYDPTEEDDEEFC FMQEMI+MMNNVKPEGDSFEDLQRMF
Sbjct: 61  VQEAYSVLSDQTKRSVYDAGLYDPTEEDDEEFCDFMQEMITMMNNVKPEGDSFEDLQRMF 120

Query: 121 MEMVGSDGIGGFTVNDNPTASKRPRANGSRSSEAKRSTMRR 162
           MEMVGSDG+G F +NDNPTASKRPR NGSRSS  KRS+ RR
Sbjct: 121 MEMVGSDGVGMFNMNDNPTASKRPRPNGSRSSAPKRSSSRR 161

[Close]

BLAST of Carg23275 vs. TAIR 10
Match: AT1G56300.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 217.2 bits (552), Expect = 9.4e-57
Identity = 109/153 (71.24%), Postives = 127/153 (83.01%), Query Frame = 0


Query: 4   GGGSDGGSSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQLVQE 63
           GGGS+  SSYY ILGIRKDAS+S+IRTAYRKLA+KWHPDR  RNP VAGEAKR+FQ +QE
Sbjct: 5   GGGSNVRSSYYTILGIRKDASVSDIRTAYRKLAMKWHPDRYARNPGVAGEAKRRFQQIQE 64

Query: 64  AYSVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGDSFEDLQRMFMEM 123
           AYSVL+D+ KRS+YD GLYDP E+DD++FC FMQEMISMMNNVK  G+S EDLQRMF +M
Sbjct: 65  AYSVLNDENKRSMYDVGLYDPHEDDDDDFCDFMQEMISMMNNVKDAGESLEDLQRMFTDM 124

Query: 124 VGSDGIGGFTVNDNPTASKRPRANGSRSSEAKR 157
           VG DG+  +  N+NP  +KRPR N SRSS A R
Sbjct: 125 VGGDGV-SYDCNNNPKGNKRPRVNISRSSAAMR 156

[Close]

BLAST of Carg23275 vs. TAIR 10
Match: AT3G14200.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 1.8e-28
Identity = 63/119 (52.94%), Postives = 84/119 (70.59%), Query Frame = 0


Query: 14  YAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQLVQEAYSVLSDQAK 73
           YA+LG++K+ S +E+R+AY+KLAL+WHPDRC+    V  EAK++FQ +QEAYSVLSD  K
Sbjct: 14  YAVLGLKKECSKTELRSAYKKLALRWHPDRCSSMEFVE-EAKKKFQAIQEAYSVLSDSNK 73

Query: 74  RSVYDAGLYDPTEEDDEEFCG-FMQEMISMMNNVKPE----GDSFEDLQRMFMEMVGSD 128
           R +YD G Y+  ++DD+   G F+ EM +MMN  KP     GDSFE LQ +F EM   D
Sbjct: 74  RFLYDVGAYNTDDDDDQNGMGDFLNEMATMMNQSKPSDNNTGDSFEQLQDLFNEMFQGD 131

[Close]

BLAST of Carg23275 vs. TAIR 10
Match: AT1G71000.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.2e-27
Identity = 66/121 (54.55%), Postives = 84/121 (69.42%), Query Frame = 0


Query: 8   DGG--SSYYAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQLVQEAY 67
           +GG   +YY ILG+  D+S  +IR AY KLA  WHPDR T++P  +GEAKR+FQ +QEAY
Sbjct: 2   EGGIRQTYYEILGVAVDSSAEQIRRAYHKLAKIWHPDRWTKDPFRSGEAKRRFQQIQEAY 61

Query: 68  SVLSDQAKRSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGD--SFEDLQRMFMEM 125
           SVLSD+ KRS YD GLYD  E  DE +  F+QEM+S+M+  K E    S E+LQ M  +M
Sbjct: 62  SVLSDERKRSSYDVGLYDSGE--DEGYFDFVQEMVSLMSQTKREEKQYSLEELQTMVDDM 120

[Close]

BLAST of Carg23275 vs. TAIR 10
Match: AT1G72416.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 90.9 bits (224), Expect = 1.0e-18
Identity = 45/115 (39.13%), Postives = 70/115 (60.87%), Query Frame = 0


Query: 14  YAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQLVQEAYSVLSDQAK 73
           YA+L +  + +  ++R +Y+ L LKWHPDR         EAK +FQ +Q AYSVLSD  K
Sbjct: 7   YAVLDLNNECTQGDLRLSYKNLVLKWHPDRFLEE-IEKDEAKMKFQSIQRAYSVLSDSNK 66

Query: 74  RSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGD-SFEDLQRMFMEMVGSD 128
           R +YD G YD +++D+     F+ EM+++M   +  GD S E+ + +F E++  D
Sbjct: 67  RLLYDVGAYD-SDDDETGMADFINEMVTLMAQTQSTGDESLEEFEELFQELLKDD 119

[Close]

BLAST of Carg23275 vs. TAIR 10
Match: AT1G72416.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 90.9 bits (224), Expect = 1.0e-18
Identity = 45/115 (39.13%), Postives = 70/115 (60.87%), Query Frame = 0


Query: 14  YAILGIRKDASLSEIRTAYRKLALKWHPDRCTRNPAVAGEAKRQFQLVQEAYSVLSDQAK 73
           YA+L +  + +  ++R +Y+ L LKWHPDR         EAK +FQ +Q AYSVLSD  K
Sbjct: 7   YAVLDLNNECTQGDLRLSYKNLVLKWHPDRFLEE-IEKDEAKMKFQSIQRAYSVLSDSNK 66

Query: 74  RSVYDAGLYDPTEEDDEEFCGFMQEMISMMNNVKPEGD-SFEDLQRMFMEMVGSD 128
           R +YD G YD +++D+     F+ EM+++M   +  GD S E+ + +F E++  D
Sbjct: 67  RLLYDVGAYD-SDDDETGMADFINEMVTLMAQTQSTGDESLEEFEELFQELLKDD 119

The following BLAST results are available for this feature:

BLAST of Carg23275 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6586232.1	3.2e-83	100.00	DnaJ-like subfamily B member 6, partial [Cucurbita argyrosperma subsp. sororia] ...	[more]
XP_023536754.1	1.1e-80	97.50	uncharacterized protein LOC111798035 [Cucurbita pepo subsp. pepo]	[more]
XP_022937579.1	1.9e-80	96.89	uncharacterized protein LOC111443946 [Cucurbita moschata] >XP_022937580.1 unchar...	[more]
XP_022965987.1	1.6e-79	95.03	dnaJ homolog subfamily B member 8 [Cucurbita maxima] >XP_022965988.1 dnaJ homolo...	[more]
XP_004144765.1	1.8e-73	89.44	dnaJ homolog subfamily B member 8 [Cucumis sativus] >KGN60978.1 hypothetical pro...	[more]

BLAST of Carg23275 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A6Q486	3.9e-15	40.83	Chaperone protein DnaJ OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=dnaJ P...	[more]
Q5SLW9	3.3e-14	45.19	Chaperone protein DnaJ 1 OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / ...	[more]
Q72GN6	3.3e-14	45.19	Chaperone protein DnaJ OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 /...	[more]
Q5F3Z5	4.3e-14	57.58	DnaJ homolog subfamily B member 6 OS=Gallus gallus OX=9031 GN=DNAJB6 PE=2 SV=1	[more]
Q8NHS0	7.3e-14	54.41	DnaJ homolog subfamily B member 8 OS=Homo sapiens OX=9606 GN=DNAJB8 PE=1 SV=1	[more]

BLAST of Carg23275 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FH54	9.4e-81	96.89	uncharacterized protein LOC111443946 OS=Cucurbita moschata OX=3662 GN=LOC1114439...	[more]
A0A6J1HLS8	8.0e-80	95.03	dnaJ homolog subfamily B member 8 OS=Cucurbita maxima OX=3661 GN=LOC111465708 PE...	[more]
A0A0A0LGH9	8.5e-74	89.44	J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033330 PE=4 SV=1	[more]
A0A6J1G400	3.2e-73	88.20	dnaJ homolog subfamily B member 8-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A5D3BDE0	4.2e-73	88.82	DnaJ-like protein subfamily B member 8 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Carg23275 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56300.1	9.4e-57	71.24	Chaperone DnaJ-domain superfamily protein	[more]
AT3G14200.1	1.8e-28	52.94	Chaperone DnaJ-domain superfamily protein	[more]
AT1G71000.1	1.2e-27	54.55	Chaperone DnaJ-domain superfamily protein	[more]
AT1G72416.1	1.0e-18	39.13	Chaperone DnaJ-domain superfamily protein	[more]
AT1G72416.2	1.0e-18	39.13	Chaperone DnaJ-domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 14..32 score: 46.68 coord: 53..73 score: 45.09 coord: 32..47 score: 52.6
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 11..73 e-value: 8.9E-26 score: 101.6
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 13..78 e-value: 1.3E-24 score: 86.1
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 12..81 score: 19.459675
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 13..70 e-value: 6.76962E-25 score: 88.7565
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 10..136 e-value: 1.8E-27 score: 98.1
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 12..84
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 131..161
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 132..147
None	No IPR available	PANTHER	PTHR44743:SF11	PUTATIVE, EXPRESSED-RELATED	coord: 11..147
None	No IPR available	PANTHER	PTHR44743	PUTATIVE, EXPRESSED-RELATED	coord: 11..147
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 58..77

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23275-RA	Carg23275-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010583	response to cyclopentenone