Carg23258 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23258
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	nicotianamine synthase-like
Location	Carg_Chr12: 9913211 .. 9914059 (-)
RNA-Seq Expression	Carg23258
Synteny	Carg23258

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTTGCGACGGCGAAGGGCTCCTTTTGGTCCAAAAGGTCTCAGAACTTTACCACAAAATCTCCACTCTCCAAAGCCTCCAACCCTCCAAACATGTCAACTCCCTCTTCTCTCAGCTCGTCCTCACCTGCACCCCTCCGCCGCCTCCTCCACCGTCTCCGCCGCTCATCGACATCTCCAAGCTCAGCTCGACCGTCCAAACCATGAGATCCCATCTCATCAAGCTCTGCGGCCAAGCCGAGGGCTTACTCGAGCACCATTACTCGACCATCTTAGCCTCCCACCAGAACCCTCTTGACCATCTCTCCCTTTTCCCTTACTACTCCAACTACCTCAAACTAACCCATCTCGAATTCTCCATTCTGACCCGTTACATATCATCCTCTCCACCGTCCAAAGTGGCATTCATTGGCTCTGGTGCTCTCCCGTTCTCGTCCATCATCATGGCTTTGAAATACCTCCCGACGACTGAGTTTCACAACTTTGATGTCGACTCATCTGCTAATGCCAAGGCTACGACGCTCGTAGCAAGCGACGTGGACTTGTCGAAGCGGATGTTGTTCCACACTACTGATATCATGGACGTCACCTCGAACATGTTGGGAGAGTTCGAGGTGGTGTTTTTGGCAGCGTTGGTGGGAATGGAGAAAGAGGAGAAAGTTAAGGTAATCGAGCATTTACGAAAGAACATGACTAGTGGCTCGTTGTTGATGTTGAGAAGCGCGTATGGTGCACGTGCATTTCTTTATCCAGTGGTGGAAGCTTCTGATCTTCGAGGGTTTGAGATTCTTACAACGTTTCATCCGACTGATGATGTTGTCAATTCGATCGTGTTGGCTCGATTATAA

mRNA sequence

ATGTGTTGCGACGGCGAAGGGCTCCTTTTGGTCCAAAAGGTCTCAGAACTTTACCACAAAATCTCCACTCTCCAAAGCCTCCAACCCTCCAAACATGTCAACTCCCTCTTCTCTCAGCTCGTCCTCACCTGCACCCCTCCGCCGCCTCCTCCACCGTCTCCGCCGCTCATCGACATCTCCAAGCTCAGCTCGACCGTCCAAACCATGAGATCCCATCTCATCAAGCTCTGCGGCCAAGCCGAGGGCTTACTCGAGCACCATTACTCGACCATCTTAGCCTCCCACCAGAACCCTCTTGACCATCTCTCCCTTTTCCCTTACTACTCCAACTACCTCAAACTAACCCATCTCGAATTCTCCATTCTGACCCGTTACATATCATCCTCTCCACCGTCCAAAGTGGCATTCATTGGCTCTGGTGCTCTCCCGTTCTCGTCCATCATCATGGCTTTGAAATACCTCCCGACGACTGAGTTTCACAACTTTGATGTCGACTCATCTGCTAATGCCAAGGCTACGACGCTCGTAGCAAGCGACGTGGACTTGTCGAAGCGGATGTTGTTCCACACTACTGATATCATGGACGTCACCTCGAACATGTTGGGAGAGTTCGAGGTGGTGTTTTTGGCAGCGTTGGTGGGAATGGAGAAAGAGGAGAAAGTTAAGGTAATCGAGCATTTACGAAAGAACATGACTAGTGGCTCGTTGTTGATGTTGAGAAGCGCGTATGGTGCACGTGCATTTCTTTATCCAGTGGTGGAAGCTTCTGATCTTCGAGGGTTTGAGATTCTTACAACGTTTCATCCGACTGATGATGTTGTCAATTCGATCGTGTTGGCTCGATTATAA

Coding sequence (CDS)

ATGTGTTGCGACGGCGAAGGGCTCCTTTTGGTCCAAAAGGTCTCAGAACTTTACCACAAAATCTCCACTCTCCAAAGCCTCCAACCCTCCAAACATGTCAACTCCCTCTTCTCTCAGCTCGTCCTCACCTGCACCCCTCCGCCGCCTCCTCCACCGTCTCCGCCGCTCATCGACATCTCCAAGCTCAGCTCGACCGTCCAAACCATGAGATCCCATCTCATCAAGCTCTGCGGCCAAGCCGAGGGCTTACTCGAGCACCATTACTCGACCATCTTAGCCTCCCACCAGAACCCTCTTGACCATCTCTCCCTTTTCCCTTACTACTCCAACTACCTCAAACTAACCCATCTCGAATTCTCCATTCTGACCCGTTACATATCATCCTCTCCACCGTCCAAAGTGGCATTCATTGGCTCTGGTGCTCTCCCGTTCTCGTCCATCATCATGGCTTTGAAATACCTCCCGACGACTGAGTTTCACAACTTTGATGTCGACTCATCTGCTAATGCCAAGGCTACGACGCTCGTAGCAAGCGACGTGGACTTGTCGAAGCGGATGTTGTTCCACACTACTGATATCATGGACGTCACCTCGAACATGTTGGGAGAGTTCGAGGTGGTGTTTTTGGCAGCGTTGGTGGGAATGGAGAAAGAGGAGAAAGTTAAGGTAATCGAGCATTTACGAAAGAACATGACTAGTGGCTCGTTGTTGATGTTGAGAAGCGCGTATGGTGCACGTGCATTTCTTTATCCAGTGGTGGAAGCTTCTGATCTTCGAGGGTTTGAGATTCTTACAACGTTTCATCCGACTGATGATGTTGTCAATTCGATCGTGTTGGCTCGATTATAA

Protein sequence

MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDVDLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL

Homology

[Close]

BLAST of Carg23258 vs. NCBI nr
Match: KAG7021049.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 547.4 bits (1409), Expect = 7.4e-152
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180
           ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180

Query: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Carg23258 vs. NCBI nr
Match: KAG6586217.1 (putative nicotianamine synthase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 542.7 bits (1397), Expect = 1.8e-150
Identity = 279/282 (98.94%), Postives = 282/282 (100.00%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQ+LQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQTLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180
           ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180

Query: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLSKRMLFHTTDIMDVTS+MLGEFEVVFLAALVGMEKEEKVKVIEHL+KNMTSGSLLMLR
Sbjct: 181 DLSKRMLFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLQKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Carg23258 vs. NCBI nr
Match: XP_023538584.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 537.7 bits (1384), Expect = 5.9e-149
Identity = 277/282 (98.23%), Postives = 278/282 (98.58%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDVS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
            LSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  NLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180
           ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKA  LVASDV
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAMALVASDV 180

Query: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLSKRMLFHTTDIMDVTSNML EFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSKRMLFHTTDIMDVTSNMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Carg23258 vs. NCBI nr
Match: XP_022938003.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 536.2 bits (1380), Expect = 1.7e-148
Identity = 277/282 (98.23%), Postives = 279/282 (98.94%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180
           ILTRYISSS PSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAT LVASDV
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATALVASDV 180

Query: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLS+RM FHTTDIMDVTS+MLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Carg23258 vs. NCBI nr
Match: XP_022965879.1 (nicotianamine synthase [Cucurbita maxima])

HSP 1 Score: 530.0 bits (1364), Expect = 1.2e-146
Identity = 274/282 (97.16%), Postives = 277/282 (98.23%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180
           ILTRYIS + PSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAT LVASDV
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180

Query: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLS+RM FHTTDIMDVTS+ML EFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Carg23258 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 2.5e-86
Identity = 161/272 (59.19%), Postives = 216/272 (79.41%), Query Frame = 0


Query: 10  LVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTM 69
           +V+KV ELY +IS L++L PSK VN LF+ LV TC PP P       ID+SKL   +Q +
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP-------IDVSKLCQKIQEI 68

Query: 70  RSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILTRYISSS 129
           RSHLIKLCGQAEGLLE H+S IL+S++NPL HL +FPY+ NY+KL+ LE++ILT+  +++
Sbjct: 69  RSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKN-TTN 128

Query: 130 PPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDVDLSKRMLFH 189
            P K+AFIGSG LP +S+++A K+L TT FHN+D+D  AN  A+ LVA+D D+S RM FH
Sbjct: 129 IPKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFH 188

Query: 190 TTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAFL 249
           T D+MDVT   L +++VVFLAALVGM+KE+KVKV++HL K M+ G+ LMLRSA+GARAFL
Sbjct: 189 TADVMDVTC-ALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFL 248

Query: 250 YPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           YPV++  DLRGFE+L+ +HPTD+V+NS+++AR
Sbjct: 249 YPVLDPRDLRGFEVLSVYHPTDEVINSVIIAR 271

[Close]

BLAST of Carg23258 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 5.6e-86
Identity = 162/273 (59.34%), Postives = 214/273 (78.39%), Query Frame = 0


Query: 10  LVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTM 69
           LV K+ +LY KIS L++L+P + V++LF QLV TC PP P       ID++K+S  +Q M
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPN------IDVTKMSENIQEM 68

Query: 70  RSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILTRYISS 129
           RS+LIK+CG+AEG LEHH+S+IL S + NPL HL+LFPYY+NYLKL+ LEF +L + ++ 
Sbjct: 69  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 128

Query: 130 SPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDVDLSKRMLF 189
             P  VAFIGSG LP +S+++A  +L  + FHNFD+D SAN  A  LV+SD DLS+RM F
Sbjct: 129 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 188

Query: 190 HTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAF 249
           HT DIMDVT ++ G F+VVFLAALVGM+K+EKVKV+EHL K+M+ G+LLMLRSA+G RAF
Sbjct: 189 HTVDIMDVTESLKG-FDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAF 248

Query: 250 LYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LYP+VE  DL GFE+L+ +HPTD+V+NSIV++R
Sbjct: 249 LYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of Carg23258 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 1.8e-84
Identity = 162/284 (57.04%), Postives = 219/284 (77.11%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           M C     L+V+++ +LY +IS L+SL+PSK+V++LF QLV TC       P+   ID++
Sbjct: 1   MACQNN--LVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL------PTDTNIDVT 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILAS---HQNPLDHLSLFPYYSNYLKLTHL 120
            +   V+ MR++LIKLCG+AEG LE H+STIL S    QNPLDHL +FPYYSNYLKL  L
Sbjct: 61  NMCEEVKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKL 120

Query: 121 EFSILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVA 180
           EF +L+++ SS  P+K+AF+GSG +P +SI++A  +LP T FHNFD+DS AN  A+ LV+
Sbjct: 121 EFDLLSQH-SSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVS 180

Query: 181 SDVDLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLL 240
            D DLSKRM+FHTTD+++ T   L +++VVFLAALVGM+KE KVK IEHL K+M  G++L
Sbjct: 181 RDPDLSKRMIFHTTDVLNATEG-LDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVL 240

Query: 241 MLRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           MLRSA+  RAFLYP+V++SDL+GF++LT +HPTDDVVNS+V+AR
Sbjct: 241 MLRSAHALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of Carg23258 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 5.3e-84
Identity = 163/273 (59.71%), Postives = 212/273 (77.66%), Query Frame = 0


Query: 10  LVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTM 69
           LVQ + +LY KIS L+SL+PS+ VN LF QLV TC PP P       ID++K+   VQ +
Sbjct: 8   LVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPN------IDVTKMCDRVQEI 67

Query: 70  RSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILTRYISS 129
           R +LIK+CG AEG LE+H+S+IL S+Q NPL HL++FPYY+NYLKL  LEF +L + ++ 
Sbjct: 68  RLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNG 127

Query: 130 SPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDVDLSKRMLF 189
             P  VAFIGSG LP +SI++A  +L  T FHNFD+D SAN+ A+ LV+SD D+S+RM F
Sbjct: 128 FVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFF 187

Query: 190 HTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAF 249
           HT DIMDVT + L  F+VVFLAALVGM KEEKVKVIEHL+K+M  G++LMLRSA+G RAF
Sbjct: 188 HTVDIMDVTES-LKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAF 247

Query: 250 LYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LYP+VE  DL+GFE+L+ +HPTDDV+NS+V+++
Sbjct: 248 LYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of Carg23258 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 4.9e-82
Identity = 159/283 (56.18%), Postives = 220/283 (77.74%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           M C  E  L+V+++ +LY++IS L+SL+PSK+V++LF QLV TC       P+   ID++
Sbjct: 1   MAC--ENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL------PTDTNIDVT 60

Query: 61  KL-SSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLE 120
           ++    V+ MRSHLIKLCG+AEG LE H+S IL S + NPL+HL +FPYY+NYLKL  LE
Sbjct: 61  EIHDEKVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLE 120

Query: 121 FSILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVAS 180
           F +L+++ ++  P+KVAFIGSG +P +SI++A  +LP T FHNFD+DS AN  A+ LV+ 
Sbjct: 121 FDLLSQH-TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSR 180

Query: 181 DVDLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLM 240
           D DLSKRM+FHTTD+++     L +++VVFLAALVGM+KE KVK IEHL K+M  G+++M
Sbjct: 181 DSDLSKRMIFHTTDVLNAKEG-LDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVM 240

Query: 241 LRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LRSA+G RAFLYP+V++ DL+GFE+LT +HP+DDVVNS+V+AR
Sbjct: 241 LRSAHGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of Carg23258 vs. ExPASy TrEMBL
Match: A0A6J1FHL8 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 8.3e-149
Identity = 277/282 (98.23%), Postives = 279/282 (98.94%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180
           ILTRYISSS PSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAT LVASDV
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATALVASDV 180

Query: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLS+RM FHTTDIMDVTS+MLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Carg23258 vs. ExPASy TrEMBL
Match: A0A6J1HS54 (nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 6.0e-147
Identity = 274/282 (97.16%), Postives = 277/282 (98.23%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180
           ILTRYIS + PSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAT LVASDV
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180

Query: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLS+RM FHTTDIMDVTS+ML EFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Carg23258 vs. ExPASy TrEMBL
Match: A0A1S3BXJ6 (nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.2e-117
Identity = 226/282 (80.14%), Postives = 249/282 (88.30%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCC  + LLLVQK+SELYHKISTL+SL+PSKHVNSLFSQLVL    PP    SP  ID+S
Sbjct: 1   MCC--QSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
            L S++QTMRS+LIKLCG+AE LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  SLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180
           IL  +I + PPSKVAF+GSGALPFSSIIMALKYLP TEFHNFD+D SANAKA+ LVA DV
Sbjct: 121 ILNHHI-TPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDV 180

Query: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           +LS+RM+FHTTDIM+V S  L EFEVVFLAALVGME+EEK KVIEHLRKNM  G+LLMLR
Sbjct: 181 ELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 279

[Close]

BLAST of Carg23258 vs. ExPASy TrEMBL
Match: A0A0A0LJ09 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 9.3e-116
Identity = 225/286 (78.67%), Postives = 250/286 (87.41%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLV-LTCTPPPPPPPSPPLIDI 60
           MCC  + LLLVQK+SELYHKISTL+SL+PSKHVNSLFSQLV L CT      PS   ID+
Sbjct: 1   MCC--QSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICT------PSSSSIDV 60

Query: 61  SKLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEF 120
           S L ST+QTMRSHLIKLCG+AE LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEF
Sbjct: 61  STLCSTIQTMRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEF 120

Query: 121 SILTRYI---SSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLV 180
           SIL  +    +++PPSKVAF+GSGALPFSSIIMALKY P TEFHNFD+D SANAKA+ LV
Sbjct: 121 SILNHHAPHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLV 180

Query: 181 ASDVDLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSL 240
           A DV+LS+RM+FHTTDIMDV S  L EFEVVFLAALVGME+EEK KVIEHLRKNM+ G+L
Sbjct: 181 AGDVELSRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGAL 240

Query: 241 LMLRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           LMLRSAYGARAF+YPVVEA DLRGF+ILT FHPTD+V+NSIVLARL
Sbjct: 241 LMLRSAYGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

[Close]

BLAST of Carg23258 vs. ExPASy TrEMBL
Match: A0A6J1DTD8 (probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309 PE=3 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 3.3e-113
Identity = 220/282 (78.01%), Postives = 245/282 (86.88%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCC  + +LLVQKVSELYHKISTLQSL+PSK VNSLFSQLVLTC    PPPPSPP ID+S
Sbjct: 1   MCC--QEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCA---PPPPSPPPIDVS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYS+ILASH+NPL++LS+FPYYSNYLKL HLEFS
Sbjct: 61  KLGSAVQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFS 120

Query: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180
           IL R+   + PSKVAF+GSGALPF+SIIMA KYLP TEFHNFDVD SANAKA  LVA D 
Sbjct: 121 ILERH---AQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDR 180

Query: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DL +RM+FHT DIM+VT  + G F+VVFLAALVGME+EEKVKVIEHL KNM  G+LLMLR
Sbjct: 181 DLERRMVFHTADIMEVTGELRG-FQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEA DL+GFEILT FHPTD+V+NS+VLARL
Sbjct: 241 SAYGARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273

[Close]

BLAST of Carg23258 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 318.9 bits (816), Expect = 4.0e-87
Identity = 162/273 (59.34%), Postives = 214/273 (78.39%), Query Frame = 0


Query: 10  LVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTM 69
           LV K+ +LY KIS L++L+P + V++LF QLV TC PP P       ID++K+S  +Q M
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPN------IDVTKMSENIQEM 68

Query: 70  RSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILTRYISS 129
           RS+LIK+CG+AEG LEHH+S+IL S + NPL HL+LFPYY+NYLKL+ LEF +L + ++ 
Sbjct: 69  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 128

Query: 130 SPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDVDLSKRMLF 189
             P  VAFIGSG LP +S+++A  +L  + FHNFD+D SAN  A  LV+SD DLS+RM F
Sbjct: 129 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 188

Query: 190 HTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAF 249
           HT DIMDVT ++ G F+VVFLAALVGM+K+EKVKV+EHL K+M+ G+LLMLRSA+G RAF
Sbjct: 189 HTVDIMDVTESLKG-FDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAF 248

Query: 250 LYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LYP+VE  DL GFE+L+ +HPTD+V+NSIV++R
Sbjct: 249 LYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of Carg23258 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 313.9 bits (803), Expect = 1.3e-85
Identity = 162/284 (57.04%), Postives = 219/284 (77.11%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           M C     L+V+++ +LY +IS L+SL+PSK+V++LF QLV TC       P+   ID++
Sbjct: 1   MACQNN--LVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL------PTDTNIDVT 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILAS---HQNPLDHLSLFPYYSNYLKLTHL 120
            +   V+ MR++LIKLCG+AEG LE H+STIL S    QNPLDHL +FPYYSNYLKL  L
Sbjct: 61  NMCEEVKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKL 120

Query: 121 EFSILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVA 180
           EF +L+++ SS  P+K+AF+GSG +P +SI++A  +LP T FHNFD+DS AN  A+ LV+
Sbjct: 121 EFDLLSQH-SSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVS 180

Query: 181 SDVDLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLL 240
            D DLSKRM+FHTTD+++ T   L +++VVFLAALVGM+KE KVK IEHL K+M  G++L
Sbjct: 181 RDPDLSKRMIFHTTDVLNATEG-LDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVL 240

Query: 241 MLRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           MLRSA+  RAFLYP+V++SDL+GF++LT +HPTDDVVNS+V+AR
Sbjct: 241 MLRSAHALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of Carg23258 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 312.4 bits (799), Expect = 3.8e-85
Identity = 163/273 (59.71%), Postives = 212/273 (77.66%), Query Frame = 0


Query: 10  LVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTM 69
           LVQ + +LY KIS L+SL+PS+ VN LF QLV TC PP P       ID++K+   VQ +
Sbjct: 8   LVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPN------IDVTKMCDRVQEI 67

Query: 70  RSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILTRYISS 129
           R +LIK+CG AEG LE+H+S+IL S+Q NPL HL++FPYY+NYLKL  LEF +L + ++ 
Sbjct: 68  RLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNG 127

Query: 130 SPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDVDLSKRMLF 189
             P  VAFIGSG LP +SI++A  +L  T FHNFD+D SAN+ A+ LV+SD D+S+RM F
Sbjct: 128 FVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFF 187

Query: 190 HTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAF 249
           HT DIMDVT + L  F+VVFLAALVGM KEEKVKVIEHL+K+M  G++LMLRSA+G RAF
Sbjct: 188 HTVDIMDVTES-LKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAF 247

Query: 250 LYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LYP+VE  DL+GFE+L+ +HPTDDV+NS+V+++
Sbjct: 248 LYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of Carg23258 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 305.8 bits (782), Expect = 3.5e-83
Identity = 159/283 (56.18%), Postives = 220/283 (77.74%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           M C  E  L+V+++ +LY++IS L+SL+PSK+V++LF QLV TC       P+   ID++
Sbjct: 1   MAC--ENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL------PTDTNIDVT 60

Query: 61  KL-SSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLE 120
           ++    V+ MRSHLIKLCG+AEG LE H+S IL S + NPL+HL +FPYY+NYLKL  LE
Sbjct: 61  EIHDEKVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLE 120

Query: 121 FSILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVAS 180
           F +L+++ ++  P+KVAFIGSG +P +SI++A  +LP T FHNFD+DS AN  A+ LV+ 
Sbjct: 121 FDLLSQH-TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSR 180

Query: 181 DVDLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLM 240
           D DLSKRM+FHTTD+++     L +++VVFLAALVGM+KE KVK IEHL K+M  G+++M
Sbjct: 181 DSDLSKRMIFHTTDVLNAKEG-LDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVM 240

Query: 241 LRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LRSA+G RAFLYP+V++ DL+GFE+LT +HP+DDVVNS+V+AR
Sbjct: 241 LRSAHGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of Carg23258 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 58.2 bits (139), Expect = 1.3e-08
Identity = 29/53 (54.72%), Postives = 39/53 (73.58%), Query Frame = 0


Query: 231 MTSGSLLMLRSAYGARAFLYPVVEASDLRGFEILTTFHP--TDDVVNSIVLAR 282
           M  G+LLMLRSA G R+FLY  V+  DL+GFE+L  +HP  +D  VNS+++AR
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVAR 53

The following BLAST results are available for this feature:

BLAST of Carg23258 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021049.1	7.4e-152	100.00	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6586217.1	1.8e-150	98.94	putative nicotianamine synthase 4, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023538584.1	5.9e-149	98.23	nicotianamine synthase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022938003.1	1.7e-148	98.23	nicotianamine synthase-like [Cucurbita moschata]	[more]
XP_022965879.1	1.2e-146	97.16	nicotianamine synthase [Cucurbita maxima]	[more]

BLAST of Carg23258 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI7	2.5e-86	59.19	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9C7X5	5.6e-86	59.34	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
Q9FF79	1.8e-84	57.04	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
O80483	5.3e-84	59.71	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FKT9	4.9e-82	56.18	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of Carg23258 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FHL8	8.3e-149	98.23	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 S...	[more]
A0A6J1HS54	6.0e-147	97.16	nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1	[more]
A0A1S3BXJ6	2.2e-117	80.14	nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1	[more]
A0A0A0LJ09	9.3e-116	78.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1	[more]
A0A6J1DTD8	3.3e-113	78.01	probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309...	[more]

BLAST of Carg23258 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	4.0e-87	59.34	nicotianamine synthase 4	[more]
AT5G04950.1	1.3e-85	57.04	nicotianamine synthase 1	[more]
AT1G09240.1	3.8e-85	59.71	nicotianamine synthase 3	[more]
AT5G56080.1	3.5e-83	56.18	nicotianamine synthase 2	[more]
AT4G26483.1	1.3e-08	54.72	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 56..282 e-value: 5.4E-59 score: 201.5
None	No IPR available	PANTHER	PTHR32266:SF17	SYNTHASE, PUTATIVE-RELATED	coord: 3..281
None	No IPR available	SUPERFAMILY	101447	Formin homology 2 domain (FH2 domain)	coord: 46..226
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 9..281 e-value: 3.6E-112 score: 374.2
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 3..281
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 2..282 score: 51.825874

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23258-RA	Carg23258-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity