Carg23127 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg23127 vs. NCBI nr
Match: KAG7025725.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 579/579 (100.00%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPK 60
           MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPK
Sbjct: 1   MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPK 60

Query: 61  PLKAIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKY 120
           PLKAIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKY
Sbjct: 61  PLKAIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKY 120

Query: 121 EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG 180
           EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG
Sbjct: 121 EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG 180

Query: 181 RKPTDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKK 240
           RKPTDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKK
Sbjct: 181 RKPTDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKK 240

Query: 241 EKDSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRE 300
           EKDSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRE
Sbjct: 241 EKDSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRE 300

Query: 301 LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI 360
           LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI
Sbjct: 301 LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI 360

Query: 361 TGKEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSPGNGISAVAFDTKA 420
           TGKEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSPGNGISAVAFDTKA
Sbjct: 361 TGKEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSPGNGISAVAFDTKA 420

Query: 421 AKFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGI 480
           AKFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGI
Sbjct: 421 AKFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGI 480

Query: 481 IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS 540
           IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS
Sbjct: 481 IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS 540

Query: 541 WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA 580
           WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA
Sbjct: 541 WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA 579

BLAST of Carg23127 vs. NCBI nr
Match: KAG6593377.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 577/579 (99.65%), Postives = 577/579 (99.65%), Query Frame = 0

Query: 1   MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPK 60
           MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIE EDNEVKVCVFNVPK
Sbjct: 26  MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEGEDNEVKVCVFNVPK 85

Query: 61  PLKAIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKY 120
           PLKAIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKY
Sbjct: 86  PLKAIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKY 145

Query: 121 EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG 180
           EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG
Sbjct: 146 EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG 205

Query: 181 RKPTDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKK 240
           RKPTDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKK
Sbjct: 206 RKPTDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKK 265

Query: 241 EKDSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRE 300
           EKDSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFE SFYPVRE
Sbjct: 266 EKDSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFEHSFYPVRE 325

Query: 301 LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI 360
           LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI
Sbjct: 326 LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI 385

Query: 361 TGKEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSPGNGISAVAFDTKA 420
           TGKEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSPGNGISAVAFDTKA
Sbjct: 386 TGKEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSPGNGISAVAFDTKA 445

Query: 421 AKFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGI 480
           AKFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGI
Sbjct: 446 AKFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGI 505

Query: 481 IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS 540
           IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS
Sbjct: 506 IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS 565

Query: 541 WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA 580
           WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA
Sbjct: 566 WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA 604

BLAST of Carg23127 vs. NCBI nr
Match: XP_022959754.1 (putative UPF0481 protein At3g02645 [Cucurbita moschata])

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 566/579 (97.75%), Postives = 574/579 (99.14%), Query Frame = 0

Query: 1   MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPK 60
           MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPK
Sbjct: 1   MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPK 60

Query: 61  PLKAIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKY 120
           PLKAIDPNSYTPQEVSIGPYH+WREELHEMRRHKIAAAKRAQ+RL+SLKF+DLVDKITKY
Sbjct: 61  PLKAIDPNSYTPQEVSIGPYHHWREELHEMRRHKIAAAKRAQDRLQSLKFRDLVDKITKY 120

Query: 121 EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG 180
           EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG
Sbjct: 121 EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG 180

Query: 181 RKPTDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKK 240
           RKPTDNAILRDIVMLENQVPLFILRNMLAYQYT VEPAQVLASMLLALYEYLSPFKGKKK
Sbjct: 181 RKPTDNAILRDIVMLENQVPLFILRNMLAYQYTSVEPAQVLASMLLALYEYLSPFKGKKK 240

Query: 241 EKDSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRE 300
           EKDSQALVPECFHLLDFLYRMITPILANPSEI+EDDHTHKEPTKQHVERNFE SFYPVRE
Sbjct: 241 EKDSQALVPECFHLLDFLYRMITPILANPSEIIEDDHTHKEPTKQHVERNFEHSFYPVRE 300

Query: 301 LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI 360
           LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI
Sbjct: 301 LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI 360

Query: 361 TGKEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSPGNGISAVAFDTKA 420
           TGKEEDDDDAPEEGSS TH KDKLPLLEEISIPSVSELTNSG+RFSPG+GISAVAFDTKA
Sbjct: 361 TGKEEDDDDAPEEGSSGTHWKDKLPLLEEISIPSVSELTNSGVRFSPGDGISAVAFDTKA 420

Query: 421 AKFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGI 480
           AKFCLPTITLDVNSEVVLRNLVAYEASKPSGG VFTRFIDLMNGIIDT+EDVELLTAAGI
Sbjct: 421 AKFCLPTITLDVNSEVVLRNLVAYEASKPSGGSVFTRFIDLMNGIIDTDEDVELLTAAGI 480

Query: 481 IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS 540
           IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS
Sbjct: 481 IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS 540

Query: 541 WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA 580
           WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA
Sbjct: 541 WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA 579

BLAST of Carg23127 vs. NCBI nr
Match: XP_023514999.1 (putative UPF0481 protein At3g02645 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1023.8 bits (2646), Expect = 5.6e-295
Identity = 524/585 (89.57%), Postives = 544/585 (92.99%), Query Frame = 0

Query: 1   MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEE-----EDNEVKVCV 60
           M F SL+SRFH LPTEH WQLTSSFDE QWVIQIRQSLED  E+EE     EDNEVKVC+
Sbjct: 1   MGFSSLESRFHSLPTEHFWQLTSSFDEKQWVIQIRQSLED-EELEEKDNGVEDNEVKVCI 60

Query: 61  FNVPKPLKAIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVD 120
           FNVPKPL AIDPNSYTP EVSIGPYH+WR EL+EM RHKIAAAKRAQNRL+S  F+DLVD
Sbjct: 61  FNVPKPLMAIDPNSYTPNEVSIGPYHHWRVELYEMGRHKIAAAKRAQNRLQSRNFRDLVD 120

Query: 121 KITKYEQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCS 180
           K+TKYEQKTR CYHK+LNL  ET AWMLAMDASFLLE LQTC ITK+YSL RTSSKLSCS
Sbjct: 121 KMTKYEQKTRACYHKFLNLNSETWAWMLAMDASFLLEFLQTCTITKEYSLSRTSSKLSCS 180

Query: 181 VEYDGRKPTDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPAQVLASMLLALYEYLSPF 240
           V+YDGRKPTDNAILRDIVMLENQ+PLFILRNMLAYQYTFVEP+QVLASMLL LY YLSPF
Sbjct: 181 VDYDGRKPTDNAILRDIVMLENQIPLFILRNMLAYQYTFVEPSQVLASMLLELYNYLSPF 240

Query: 241 KGKKKEKDSQALVPECFHLLDFLYRMITPILA-NPSEIMEDDHTHKEPTKQHVERNFERS 300
           KGKK+ KD QALVPECFHLLDFLYRMITPILA NPSEIMEDDH HKEPTKQHVERNF+ S
Sbjct: 241 KGKKEVKDVQALVPECFHLLDFLYRMITPILANNPSEIMEDDHNHKEPTKQHVERNFKHS 300

Query: 301 FYPVRELGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLE 360
           FYPVRELGKQIWKILSKLNSGPAHLYRIIVG RPLQVIFKLPWTIISKLPGIGILLKPLE
Sbjct: 301 FYPVRELGKQIWKILSKLNSGPAHLYRIIVGSRPLQVIFKLPWTIISKLPGIGILLKPLE 360

Query: 361 HLCSLITGKEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSPGNGISAV 420
           HLCSLITGKEEDDDD+PEEGSSRTHGKDKLPLLEEISIPSVSELT SG+RFS  +GISAV
Sbjct: 361 HLCSLITGKEEDDDDSPEEGSSRTHGKDKLPLLEEISIPSVSELTKSGVRFSRSDGISAV 420

Query: 421 AFDTKAAKFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVEL 480
           AFDTKA KF LPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRF+DLMNGIIDTEEDVEL
Sbjct: 421 AFDTKAVKFHLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFVDLMNGIIDTEEDVEL 480

Query: 481 LTAAGIIRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVK 540
           LTAAGIIRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVN+YYSSRWKVKA KLVK
Sbjct: 481 LTAAGIIRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNRYYSSRWKVKAMKLVK 540

Query: 541 KYVFGSWPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA 580
           KYVF SWP LTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA
Sbjct: 541 KYVFASWPLLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA 584

BLAST of Carg23127 vs. NCBI nr
Match: XP_011659516.1 (putative UPF0481 protein At3g02645 [Cucumis sativus] >KAE8646429.1 hypothetical protein Csa_015865 [Cucumis sativus])

HSP 1 Score: 735.3 bits (1897), Expect = 4.0e-208
Identity = 388/576 (67.36%), Postives = 457/576 (79.34%), Query Frame = 0

Query: 4   FSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPKPLK 63
           FS  SR H LP  +SW L S FDE +WVIQIRQSL++  E  EED  + VC+FNVPK L 
Sbjct: 3   FSSKSRLHSLPAGNSWGLNSDFDEERWVIQIRQSLDE--EELEEDTGIPVCIFNVPKSLM 62

Query: 64  AIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKYEQK 123
            IDP+SY PQEV+IGPYH+WR+EL+EM R+KIAAAKRAQ +L+SLKF DLV+K+TK+EQK
Sbjct: 63  VIDPDSYIPQEVAIGPYHHWRQELYEMERYKIAAAKRAQKQLQSLKFHDLVEKLTKHEQK 122

Query: 124 TRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDGRKP 183
           TR CYHKYLN   ET AWM+A+DASFLLEVL+     ++ S+   SSKLS  V+Y+GRK 
Sbjct: 123 TRACYHKYLNFNSETFAWMMAVDASFLLEVLRVYT-REETSISSVSSKLSYLVDYEGRKS 182

Query: 184 TDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPA-QVLASMLLALYEYLSPFKGKKKEK 243
             NAILRDIVMLENQ+PLF+LR ML  Q++ VEPA Q+L SMLL LYE+LSPFK  +   
Sbjct: 183 AHNAILRDIVMLENQIPLFVLRKMLELQFSAVEPADQLLLSMLLGLYEHLSPFKVMEDLV 242

Query: 244 DSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRELG 303
           + Q  V ECFHLLDFLYR+ITP LA+  E MEDD   +EP  + VE  F+    P+  LG
Sbjct: 243 ELQVSVSECFHLLDFLYRIITPKLADTLETMEDDQNQQEPAIEIVESTFKHPCSPLSSLG 302

Query: 304 KQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLITG 363
            +IWKILSKLN GP HL++ I G RPL VIFKLPWTI+S +PGIGIL+KPLE++ SL  G
Sbjct: 303 SEIWKILSKLNKGPVHLFKRIAGSRPLLVIFKLPWTIVSNIPGIGILMKPLEYIFSLKKG 362

Query: 364 KEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSP-GNGISAVAFDTKAA 423
           +EE+D +  + GSSR  GK +LPLLEEI+IPSVSELT SG+ F P G G+SA+AFD+KA 
Sbjct: 363 EEENDPE--KGGSSRKDGKIRLPLLEEITIPSVSELTKSGVLFLPIGGGVSAIAFDSKAV 422

Query: 424 KFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGII 483
            F LPTI LDVNSEVVLRNLVAYEASK SG LVFTRFI+LMNGIID+EEDV+LL   GII
Sbjct: 423 IFNLPTIKLDVNSEVVLRNLVAYEASKSSGPLVFTRFIELMNGIIDSEEDVKLLREKGII 482

Query: 484 RNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGSW 543
            NHLKS+AEV +LWNGMSKSIK TKVPFLDK IEDVNK+YS+RWKVKA+K V+KYVFGSW
Sbjct: 483 LNHLKSDAEVAELWNGMSKSIKLTKVPFLDKVIEDVNKHYSNRWKVKAAKFVEKYVFGSW 542

Query: 544 PFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTA 578
           P L LLATILLLA+ ALQAFCSVYSCSRF  +L+TA
Sbjct: 543 PLLALLATILLLAMNALQAFCSVYSCSRFFNHLSTA 573

BLAST of Carg23127 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 3.8e-113
Identity = 241/548 (43.98%), Postives = 335/548 (61.13%), Query Frame = 0

Query: 25  FDENQWVIQIRQSLEDVGEIEEED-NEVKVCVFNVPKPLKAIDPNSYTPQEVSIGPYHYW 84
           FDE +WVI +++SL+   E+EE D  EV V +FNVPK L    P+SYTP  VSIGPYH  
Sbjct: 16  FDETRWVINVQKSLD--AELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCL 75

Query: 85  REELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKYEQKTRECYHKYLNLKGETLAWML 144
           + ELHEM R+K+  A++ +N+  S +F DLV+K+   E K R CYHKY+   GETL W++
Sbjct: 76  KPELHEMERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIM 135

Query: 145 AMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDGRKPTDNAILRDIVMLENQVPLFI 204
           A+D+SFL+E L+  +  K  +L                +   N ILRDI+M+ENQ+PLF+
Sbjct: 136 AVDSSFLIEFLKIYSFRKVETLI--------------NRVGHNEILRDIMMIENQIPLFV 195

Query: 205 LRNMLAYQYTFVEPA-QVLASMLLALYEYLSPFKGK-KKEKDSQALVPECFHLLDFLYRM 264
           LR  L +Q    E A  +L S+L  L + LSP   K   ++  +A   EC H+LDFLY+M
Sbjct: 196 LRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILDFLYQM 255

Query: 265 ITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRELGKQIWKILSKLNSGPAHLYR 324
           I P +    E+ EDD  ++       E    R+   + E+                H ++
Sbjct: 256 IVPRI-EEEELEEDDEENRAD-----ENGGNRAIRFMDEI---------------KHQFK 315

Query: 325 IIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLITGKEEDDDDAPEEGSSRTHGK 384
            +   RP  +I + PW IIS LPG   L    ++L +    ++E++     + S      
Sbjct: 316 RVFASRPADLILRFPWRIISNLPGFMALKLSADYLFT----RQENEATTTRQESVSILDI 375

Query: 385 DKLPLLEEISIPSVSELTNSGIRFSP---GNGISAVAFDTKAAKFCLPTITLDVNSEVVL 444
           +K PL+EE++IPSVS+L  +G+RF P   GN IS V FD+ + +F LP I LD+N+E VL
Sbjct: 376 EKPPLVEELTIPSVSDLHKAGVRFKPTAHGN-ISTVTFDSNSGQFYLPVINLDINTETVL 435

Query: 445 RNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGIIRNHLKSNAEVTKLWNGM 504
           RNLVAYEA+  SG LVFTR+ +L+NGIID+EEDV LL   G++ + LKS+ E  ++WNGM
Sbjct: 436 RNLVAYEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGM 495

Query: 505 SKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGSWPFLTLLATILLLALTAL 564
           SKS++ TKV FLDK IEDVN+YY+ RWKVK  +LV+ YV+GSW  L  LA +LLL L +L
Sbjct: 496 SKSVRLTKVGFLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLMLVSL 521

Query: 565 QAFCSVYS 567
           Q F  V+S
Sbjct: 556 QLFSLVFS 521

BLAST of Carg23127 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 6.1e-10
Identity = 52/170 (30.59%), Postives = 83/170 (48.82%), Query Frame = 0

Query: 46  EEDNEVKVCVFNVPKPLKAIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRL 105
           E   +   C+F VP+   A++P +Y P+ VSIGPYHY  + L  +++HK    +  Q  L
Sbjct: 39  ESAGKESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHK---PRLLQLFL 98

Query: 106 ESLKFQD-----LVDKITKYEQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAIT 165
           +  K +D     LV  +   E K R+ Y + L   G  L +M+ +D  F+L V    +  
Sbjct: 99  DEAKKKDVEENVLVKAVVDLEDKIRKSYSEELK-TGHDLMFMMVLDGCFILMVFLIMSGN 158

Query: 166 KKYS---LFRTSSKLSCSVEYDGRKPTDNAILRDIVMLENQVPLFILRNM 208
            + S   +F     LS             +I  D+++LENQVP F+L+ +
Sbjct: 159 IELSEDPIFSIPWLLS-------------SIQSDLLLLENQVPFFVLQTL 191

BLAST of Carg23127 vs. ExPASy TrEMBL
Match: A0A6J1H904 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111460732 PE=4 SV=1)

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 566/579 (97.75%), Postives = 574/579 (99.14%), Query Frame = 0

Query: 1   MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPK 60
           MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPK
Sbjct: 1   MSFFSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPK 60

Query: 61  PLKAIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKY 120
           PLKAIDPNSYTPQEVSIGPYH+WREELHEMRRHKIAAAKRAQ+RL+SLKF+DLVDKITKY
Sbjct: 61  PLKAIDPNSYTPQEVSIGPYHHWREELHEMRRHKIAAAKRAQDRLQSLKFRDLVDKITKY 120

Query: 121 EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG 180
           EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG
Sbjct: 121 EQKTRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDG 180

Query: 181 RKPTDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKK 240
           RKPTDNAILRDIVMLENQVPLFILRNMLAYQYT VEPAQVLASMLLALYEYLSPFKGKKK
Sbjct: 181 RKPTDNAILRDIVMLENQVPLFILRNMLAYQYTSVEPAQVLASMLLALYEYLSPFKGKKK 240

Query: 241 EKDSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRE 300
           EKDSQALVPECFHLLDFLYRMITPILANPSEI+EDDHTHKEPTKQHVERNFE SFYPVRE
Sbjct: 241 EKDSQALVPECFHLLDFLYRMITPILANPSEIIEDDHTHKEPTKQHVERNFEHSFYPVRE 300

Query: 301 LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI 360
           LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI
Sbjct: 301 LGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLI 360

Query: 361 TGKEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSPGNGISAVAFDTKA 420
           TGKEEDDDDAPEEGSS TH KDKLPLLEEISIPSVSELTNSG+RFSPG+GISAVAFDTKA
Sbjct: 361 TGKEEDDDDAPEEGSSGTHWKDKLPLLEEISIPSVSELTNSGVRFSPGDGISAVAFDTKA 420

Query: 421 AKFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGI 480
           AKFCLPTITLDVNSEVVLRNLVAYEASKPSGG VFTRFIDLMNGIIDT+EDVELLTAAGI
Sbjct: 421 AKFCLPTITLDVNSEVVLRNLVAYEASKPSGGSVFTRFIDLMNGIIDTDEDVELLTAAGI 480

Query: 481 IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS 540
           IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS
Sbjct: 481 IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS 540

Query: 541 WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA 580
           WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA
Sbjct: 541 WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTAEA 579

BLAST of Carg23127 vs. ExPASy TrEMBL
Match: A0A5A7UU59 (Putative UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001980 PE=4 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 3.6e-207
Identity = 389/576 (67.53%), Postives = 454/576 (78.82%), Query Frame = 0

Query: 4   FSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPKPLK 63
           FS  SR H LP  +SW L S FDE +WVIQIRQSL++  E  EED  + VC+FNVPK L 
Sbjct: 3   FSSKSRLHSLPAGNSWGLNSGFDEERWVIQIRQSLDE--EELEEDIGIPVCIFNVPKSLM 62

Query: 64  AIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKYEQK 123
            IDP+SYTPQEV+IGPYH+WR+EL+EM R+KIAAAKRAQ +L+SLKF DLV+K+TK+EQK
Sbjct: 63  VIDPDSYTPQEVAIGPYHHWRQELYEMERYKIAAAKRAQKQLQSLKFHDLVEKLTKHEQK 122

Query: 124 TRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDGRKP 183
           TR CYHKYLN   ET AWM+A+DASFLLEVL+     ++ S+   SSKLS  V+Y+GRK 
Sbjct: 123 TRACYHKYLNFNSETFAWMMAVDASFLLEVLRVYT-REETSISSVSSKLSYLVDYEGRKS 182

Query: 184 TDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPA-QVLASMLLALYEYLSPFKGKKKEK 243
             NAILRDIVMLENQ+PLF+LR ML  Q++ VEPA Q+L SMLL LYE LSPFK  +   
Sbjct: 183 AHNAILRDIVMLENQIPLFVLRMMLELQFSAVEPADQLLLSMLLGLYEDLSPFKVMEDLV 242

Query: 244 DSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRELG 303
           + Q  V ECFHLLDFLYRMITP L +  E MEDD   +EP  + VE  F+    P+  LG
Sbjct: 243 ELQVSVSECFHLLDFLYRMITPKLVDTLETMEDDQNQQEPAIEIVESTFKHPCSPLSSLG 302

Query: 304 KQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLITG 363
            +IWKILSKLN GP H ++ IVG RPLQVIFKLPWTI+S +PGIG L+KPLE++ SL   
Sbjct: 303 SEIWKILSKLNEGPVHFFKRIVGSRPLQVIFKLPWTIVSNIPGIGTLMKPLEYIFSL--R 362

Query: 364 KEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSPGN-GISAVAFDTKAA 423
           K E++ D  + GSSR  GK KLPLLEEI+IPSVSELT SG+RF P N G+SA+AFD+KA 
Sbjct: 363 KVEEEKDPEKGGSSRKDGKTKLPLLEEITIPSVSELTKSGVRFLPINGGVSAIAFDSKAV 422

Query: 424 KFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGII 483
            F LP I LDVNSEVVLRNLVAYEASK SG LVFTRFI+LMNGIID+EEDV+LL   GII
Sbjct: 423 IFNLPIIKLDVNSEVVLRNLVAYEASKSSGPLVFTRFIELMNGIIDSEEDVKLLKEKGII 482

Query: 484 RNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGSW 543
            NHLKS+AEV ++WNGMSKSIK TKVPFLDK IEDVNK+YSSRWKVKA+K V+KYVFGSW
Sbjct: 483 LNHLKSDAEVAEVWNGMSKSIKLTKVPFLDKVIEDVNKHYSSRWKVKAAKFVEKYVFGSW 542

Query: 544 PFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTA 578
           P L LLATILLLA+ ALQAFCSVYSCSRF  +L+TA
Sbjct: 543 PLLALLATILLLAMNALQAFCSVYSCSRFFDHLSTA 573

BLAST of Carg23127 vs. ExPASy TrEMBL
Match: A0A1S3CDL4 (putative UPF0481 protein At3g02645 OS=Cucumis melo OX=3656 GN=LOC103499824 PE=4 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 8.0e-207
Identity = 389/576 (67.53%), Postives = 454/576 (78.82%), Query Frame = 0

Query: 4   FSLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPKPLK 63
           FS  SR H LP  +SW L S FDE +WVIQIRQSL++  E  EED  + VC+FNVPK L 
Sbjct: 3   FSSKSRLHSLPAGNSWGLNSGFDEERWVIQIRQSLDE--EELEEDIGIPVCIFNVPKSLM 62

Query: 64  AIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKYEQK 123
            IDP+SYTPQEV+IGPYH+WR+EL+EM R+KIAAAKRAQ +L+SLKF DLV+K+TK+EQK
Sbjct: 63  VIDPDSYTPQEVAIGPYHHWRQELYEMERYKIAAAKRAQKQLQSLKFHDLVEKLTKHEQK 122

Query: 124 TRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDGRKP 183
           TR CYHKYLN   ET AWM+A+DASFLLEVL+     ++ S+   SSKLS  V+Y+GRK 
Sbjct: 123 TRACYHKYLNFNSETFAWMMAVDASFLLEVLRVYT-REETSISSVSSKLSYLVDYEGRKS 182

Query: 184 TDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPA-QVLASMLLALYEYLSPFKGKKKEK 243
             NAILRDIVMLENQ+PLF+LR ML  Q++ VEPA Q+L SMLL LYE LSPFK  +   
Sbjct: 183 AHNAILRDIVMLENQIPLFVLRMMLELQFSAVEPADQLLLSMLLGLYEDLSPFKVMEDLV 242

Query: 244 DSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRELG 303
           + Q  V ECFHLLDFLYRMITP L +  E MEDD   +EP  + VE  F+    P+  LG
Sbjct: 243 ELQVSVSECFHLLDFLYRMITPKLVDTLETMEDDQNQQEPAIEIVESTFKHPCSPLSSLG 302

Query: 304 KQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLITG 363
            +IWKILSKLN GP HL++ IVG RPLQVIFKLPWTI+S +PGIG L+KPLE++ SL   
Sbjct: 303 SEIWKILSKLNEGPVHLFKRIVGSRPLQVIFKLPWTIVSNIPGIGTLMKPLEYIFSL--R 362

Query: 364 KEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSPGN-GISAVAFDTKAA 423
           K E++ D  + GSSR  G  KLPLLEEI+IPSVSELT SG+ F P N G+SA+AFD+KA 
Sbjct: 363 KVEEEKDPEKGGSSRKDGITKLPLLEEITIPSVSELTKSGVHFLPINGGVSAIAFDSKAV 422

Query: 424 KFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGII 483
            F LPTI LDVNSEVVLRNLVAYEASK SG LVFTRFI+LMNGIID+EEDV+LL   GII
Sbjct: 423 IFNLPTIKLDVNSEVVLRNLVAYEASKSSGPLVFTRFIELMNGIIDSEEDVKLLKEKGII 482

Query: 484 RNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGSW 543
            NHLKS+AEV ++WNGMSKSIK TKVPFLDK IEDVNK+YSSRWKVKA+K V+KYVFGSW
Sbjct: 483 LNHLKSDAEVAEVWNGMSKSIKLTKVPFLDKVIEDVNKHYSSRWKVKAAKFVEKYVFGSW 542

Query: 544 PFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTA 578
           P L LLATILLLA+ ALQAFCSVYSCSRF  +L+TA
Sbjct: 543 PLLALLATILLLAMNALQAFCSVYSCSRFFDHLSTA 573

BLAST of Carg23127 vs. ExPASy TrEMBL
Match: A0A6J1D655 (putative UPF0481 protein At3g02645 OS=Momordica charantia OX=3673 GN=LOC111017403 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 1.6e-194
Identity = 358/517 (69.25%), Postives = 421/517 (81.43%), Query Frame = 0

Query: 64  AIDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKYEQK 123
           AIDP+SYTPQEV+IGPYHYWR+EL+EM R+KIAAAKRAQ +L+S KF ++V+K+TKYE+K
Sbjct: 2   AIDPDSYTPQEVAIGPYHYWRQELYEMERYKIAAAKRAQKQLQSPKFHNIVEKLTKYERK 61

Query: 124 TRECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDGRKP 183
            R CYHKYLN   ETL+WM+A+DASFLLE+L+   I ++ +L R SSKLS  V+Y+G K 
Sbjct: 62  IRSCYHKYLNFNSETLSWMMAIDASFLLEILRVYTIREEKNLSRVSSKLSNLVDYEGWKS 121

Query: 184 TDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPA--QVLASMLLALYEYLSPFKGKKKE 243
             NAILRDIVMLENQ+PLF++R ML  Q++ +EPA  Q L SMLL LYE LSPFK  +  
Sbjct: 122 AYNAILRDIVMLENQIPLFVMRKMLELQFSALEPAADQKLLSMLLGLYEDLSPFKVMEDL 181

Query: 244 KDSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVREL 303
            + QA V ECFHLLDFLYRMITP LA+P EIMEDD   KEPT++++E   +  F P+ +L
Sbjct: 182 VNIQASVSECFHLLDFLYRMITPKLADPLEIMEDDQNQKEPTRENIESALKHLFSPLSKL 241

Query: 304 GKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLIT 363
           G +IWKILSKLN GP HL R ++  RPLQVIFKLPWT+ISKLPGIGIL+KPLEHLC    
Sbjct: 242 GSEIWKILSKLNRGPVHLLRRLLSSRPLQVIFKLPWTVISKLPGIGILIKPLEHLCLQRR 301

Query: 364 GKEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSP-GNGISAVAFDTKA 423
           GKEE+D   PE+GSSR+ GK + PLLEEI+IPSVS+L  SG+   P   G+SAVAFDTKA
Sbjct: 302 GKEEND---PEKGSSRSDGKPRPPLLEEITIPSVSQLVKSGVCLLPIDGGVSAVAFDTKA 361

Query: 424 AKFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGI 483
             F LPTITLDVNSEVVLRNLVAYEASKP G LVFTRFI+LMNGIID+EEDV+LL   GI
Sbjct: 362 VIFYLPTITLDVNSEVVLRNLVAYEASKPFGPLVFTRFIELMNGIIDSEEDVKLLKEKGI 421

Query: 484 IRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGS 543
           I NHLKS+AEV +LWNGMSKSIK TKVPFLDK IEDVNK+YSSRWKVKA K ++KYVFGS
Sbjct: 422 IMNHLKSDAEVAELWNGMSKSIKLTKVPFLDKVIEDVNKHYSSRWKVKAVKFIEKYVFGS 481

Query: 544 WPFLTLLATILLLALTALQAFCSVYSCSRFSRYLNTA 578
           WP L LLATILLLA+TALQAFCSVY+CS+   +LNTA
Sbjct: 482 WPLLALLATILLLAMTALQAFCSVYTCSQVFYHLNTA 515

BLAST of Carg23127 vs. ExPASy TrEMBL
Match: A0A1S4E348 (putative UPF0481 protein At3g02645 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499825 PE=4 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 9.6e-192
Identity = 376/577 (65.16%), Postives = 429/577 (74.35%), Query Frame = 0

Query: 5   SLDSRFHFLPTEHSWQLTSSFDENQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPKPLKA 64
           S  SR H LP  H W L  S++E  WV QIRQS+++  E  EED     C+  VPK L  
Sbjct: 4   SSKSRLHSLPAGHYWGLNLSYEEG-WVNQIRQSIDE--EELEEDIGHPACICTVPKSLIV 63

Query: 65  IDPNSYTPQEVSIGPYHYWREELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKYEQKT 124
            DP+SYTPQEV+IGPYH+WR+EL+ M R+KIAAAK+AQ +L+SLKF +LV+K+ KYE+K 
Sbjct: 64  FDPDSYTPQEVAIGPYHHWRQELYVMERYKIAAAKKAQKQLQSLKFHNLVEKLAKYERKI 123

Query: 125 RECYHKYLNLKGETLAWMLAMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYD--GRK 184
           R  YHKYLN   ET  WM+A+DASFLLEVLQ   I ++ S+ R SSKLSC V  D  GR+
Sbjct: 124 RAYYHKYLNFNSETFVWMMAIDASFLLEVLQVYTIREEKSISRISSKLSCLVVVDNEGRR 183

Query: 185 PTDNAILRDIVMLENQVPLFILRNMLAYQYTFVEPA-QVLASMLLALYEYLSPFKGKKKE 244
              N ILRDIVMLENQ+PLF+LR ML  Q   +E   Q+L SMLL LYE LSPF    + 
Sbjct: 184 SAQNTILRDIVMLENQIPLFVLRKMLKLQSPALEQTDQLLLSMLLGLYEDLSPF----EM 243

Query: 245 KDSQALVPECFHLLDFLYRMITPILANPSEIMEDDHTHKEPTKQHV--ERNFERSFYPVR 304
            + Q  V ECFHLLDFLYRMITP LA P EI+E+D   KE TK++   E  F+     + 
Sbjct: 244 LEPQVSVSECFHLLDFLYRMITPKLAGPLEILENDRNQKESTKENAEDENAFKHFCRSLS 303

Query: 305 ELGKQIWKILSKLNSGPAHLYRIIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSL 364
           ELG  IWKILSK N GP HL+R I+  RPLQVIFKLPWTI+SKLPGI IL+KPL HLCSL
Sbjct: 304 ELGSAIWKILSKFNKGPVHLFRRILSSRPLQVIFKLPWTIVSKLPGIVILMKPLSHLCSL 363

Query: 365 ITGKEEDDDDAPEEGSSRTHGKDKLPLLEEISIPSVSELTNSGIRFSP-GNGISAVAFDT 424
             G+EE+D    E+GSS   GK KLPL EEI+IPSVS+LT SG+ FS    G+SAVAFD 
Sbjct: 364 RKGEEEND---IEKGSSWKVGKIKLPLSEEIAIPSVSQLTKSGVHFSSIDGGVSAVAFDP 423

Query: 425 KAAKFCLPTITLDVNSEVVLRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAA 484
            A  F LPTI LDVNSEVVLRNLVAYEASK SG LVFTRFI+LMNGIID+EEDV LL   
Sbjct: 424 NAVIFYLPTINLDVNSEVVLRNLVAYEASKASGPLVFTRFIELMNGIIDSEEDVRLLKEK 483

Query: 485 GIIRNHLKSNAEVTKLWNGMSKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVF 544
           GII NHLKS+AEV  LWNGMSKSIK TKVPFLDK IEDVNKYYS RWKVKA+K VKKYVF
Sbjct: 484 GIILNHLKSDAEVADLWNGMSKSIKLTKVPFLDKVIEDVNKYYSGRWKVKAAKFVKKYVF 543

Query: 545 GSWPFLTLLATILLLALTALQAFCSVYSCSRFSRYLN 576
           GSWP L  LATILLLALTALQAFCSVYSCSRF  +LN
Sbjct: 544 GSWPLLAFLATILLLALTALQAFCSVYSCSRFIHHLN 570

BLAST of Carg23127 vs. TAIR 10
Match: AT3G02645.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 410.2 bits (1053), Expect = 2.7e-114
Identity = 241/548 (43.98%), Postives = 335/548 (61.13%), Query Frame = 0

Query: 25  FDENQWVIQIRQSLEDVGEIEEED-NEVKVCVFNVPKPLKAIDPNSYTPQEVSIGPYHYW 84
           FDE +WVI +++SL+   E+EE D  EV V +FNVPK L    P+SYTP  VSIGPYH  
Sbjct: 16  FDETRWVINVQKSLD--AELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCL 75

Query: 85  REELHEMRRHKIAAAKRAQNRLESLKFQDLVDKITKYEQKTRECYHKYLNLKGETLAWML 144
           + ELHEM R+K+  A++ +N+  S +F DLV+K+   E K R CYHKY+   GETL W++
Sbjct: 76  KPELHEMERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIM 135

Query: 145 AMDASFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDGRKPTDNAILRDIVMLENQVPLFI 204
           A+D+SFL+E L+  +  K  +L                +   N ILRDI+M+ENQ+PLF+
Sbjct: 136 AVDSSFLIEFLKIYSFRKVETLI--------------NRVGHNEILRDIMMIENQIPLFV 195

Query: 205 LRNMLAYQYTFVEPA-QVLASMLLALYEYLSPFKGK-KKEKDSQALVPECFHLLDFLYRM 264
           LR  L +Q    E A  +L S+L  L + LSP   K   ++  +A   EC H+LDFLY+M
Sbjct: 196 LRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILDFLYQM 255

Query: 265 ITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRELGKQIWKILSKLNSGPAHLYR 324
           I P +    E+ EDD  ++       E    R+   + E+                H ++
Sbjct: 256 IVPRI-EEEELEEDDEENRAD-----ENGGNRAIRFMDEI---------------KHQFK 315

Query: 325 IIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLITGKEEDDDDAPEEGSSRTHGK 384
            +   RP  +I + PW IIS LPG   L    ++L +    ++E++     + S      
Sbjct: 316 RVFASRPADLILRFPWRIISNLPGFMALKLSADYLFT----RQENEATTTRQESVSILDI 375

Query: 385 DKLPLLEEISIPSVSELTNSGIRFSP---GNGISAVAFDTKAAKFCLPTITLDVNSEVVL 444
           +K PL+EE++IPSVS+L  +G+RF P   GN IS V FD+ + +F LP I LD+N+E VL
Sbjct: 376 EKPPLVEELTIPSVSDLHKAGVRFKPTAHGN-ISTVTFDSNSGQFYLPVINLDINTETVL 435

Query: 445 RNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGIIRNHLKSNAEVTKLWNGM 504
           RNLVAYEA+  SG LVFTR+ +L+NGIID+EEDV LL   G++ + LKS+ E  ++WNGM
Sbjct: 436 RNLVAYEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGM 495

Query: 505 SKSIKSTKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGSWPFLTLLATILLLALTAL 564
           SKS++ TKV FLDK IEDVN+YY+ RWKVK  +LV+ YV+GSW  L  LA +LLL L +L
Sbjct: 496 SKSVRLTKVGFLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLMLVSL 521

Query: 565 QAFCSVYS 567
           Q F  V+S
Sbjct: 556 QLFSLVFS 521

BLAST of Carg23127 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 145.2 bits (365), Expect = 1.6e-34
Identity = 134/546 (24.54%), Postives = 225/546 (41.21%), Query Frame = 0

Query: 28  NQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPKPLKAIDPNSYTPQEVSIGPYHYWREEL 87
           + WVI IR  LE     ++     K+C++ VP  L+  D  SY PQ VS+GPYH+ ++ L
Sbjct: 88  DSWVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQENDKKSYFPQTVSLGPYHHGKKRL 147

Query: 88  HEMRRHKIAAAKRAQNRLESLKFQDLVDKITKYEQKTRECYHKYLNLKGETLAWMLAMDA 147
             M RHK  A  +   RL+  + +   + + + E+K R CY   ++L       ML +D 
Sbjct: 148 RPMERHKWRALNKVLKRLKQ-RIEMYTNAMRELEEKARACYEGPISLSRNEFTEMLVLDG 207

Query: 148 SFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDGRKPT------DNAILRDIVMLENQVPL 207
            F+LE            LFR + +    + Y    P        ++I RD++MLENQ+PL
Sbjct: 208 CFVLE------------LFRGTVEGFTEIGYARNDPVFAMRGLMHSIQRDMIMLENQLPL 267

Query: 208 FILRNMLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKKEKDSQALVPECFHLLDFLYRM 267
           F+L  +L  Q        ++A + +  ++ L P  G+   K  Q+       L+++L + 
Sbjct: 268 FVLDRLLELQLGTQNQTGIVAHVAVKFFDPLMP-TGEALTKPDQS------KLMNWLEKS 327

Query: 268 ITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRELGKQIWKILSKLNSGPAHLYR 327
           +   L +  E+             H    F RS                 L S P     
Sbjct: 328 L-DTLGDKGEL-------------HCLDVFRRSL----------------LQSSPT---- 387

Query: 328 IIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLITGKEEDDDDAPEEGSSRTHGK 387
                                 P    LLK L     ++  +++                
Sbjct: 388 ----------------------PNTRSLLKRLTRNTRVVDKRQQQ--------------- 447

Query: 388 DKLPLLEEISIPSVSELTNSGIRFSPGNGISAVAFDTKAAKFCLPTITLDVNSEVVLRNL 447
                     +  V+EL  +G++F           + K     +P + +   ++ +  NL
Sbjct: 448 ---------LVHCVTELREAGVKFRKRKTDRFWDIEFKNGYLEIPKLLIHDGTKSLFSNL 507

Query: 448 VAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGIIRNHLKSNAEVTKLWNGMSKS 507
           +A+E          T +I  M+ +I++ EDV  L   GII + L S++EV  L+N + + 
Sbjct: 508 IAFEQCHIESSNHITSYIIFMDNLINSSEDVSYLHYCGIIEHWLGSDSEVADLFNRLCQE 533

Query: 508 IK-STKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGSWPFLTLLATILLLALTALQA 567
           +    K   L +   DVN+YY+ +W V  + L  KY    W + +  A ++LL LT  Q+
Sbjct: 568 VVFDPKDSHLSRLSGDVNRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLLLTLCQS 533

BLAST of Carg23127 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 132.9 bits (333), Expect = 8.4e-31
Identity = 133/546 (24.36%), Postives = 218/546 (39.93%), Query Frame = 0

Query: 28  NQWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPKPLKAIDPNSYTPQEVSIGPYHYWREEL 87
           + WVI I   LE     ++     K+C++ VP  L+  D  SY PQ VS+GPYH+ ++ L
Sbjct: 78  DDWVISITDKLEQAHRDDDTTLWGKLCIYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRL 137

Query: 88  HEMRRHKIAAAKRAQNRLESLKFQDLVDKITKYEQKTRECYHKYLNLKGETLAWMLAMDA 147
             M RHK  A  R   R      +  +D + + E+K R CY   L+L       ML +D 
Sbjct: 138 RSMDRHKWRAVNRVLKRTNQ-GIKMYIDAMRELEEKARACYEGPLSLSSNEFIEMLVLDG 197

Query: 148 SFLLEVLQTCAITKKYSLFRTSSKLSCSVEYDGRKP------TDNAILRDIVMLENQVPL 207
            F+LE            LFR + +    + Y    P      + ++I RD+VMLENQ+PL
Sbjct: 198 CFVLE------------LFRGAVEGFTELGYARNDPVFAMRGSMHSIQRDMVMLENQLPL 257

Query: 208 FILRNMLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKKEKDSQALVPECFHLLDFLYRM 267
           F+L  +L  Q        ++A + +  ++ L P                           
Sbjct: 258 FVLNRLLELQLGTRNQTGLVAQLAIRFFDPLMP--------------------------- 317

Query: 268 ITPILANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRELGKQIWKILSKLNSGPAHLYR 327
                       ++  T    +K       ++SF P  ++G+                  
Sbjct: 318 -----------TDEPLTKSGQSKLENSLARDKSFDPFADMGE------------------ 377

Query: 328 IIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLITGKEEDDDDAPEEGSSRTHGK 387
                  L  +     +++   P      KP   L               +  S  T   
Sbjct: 378 -------LHCLDVFRRSLLRSSP------KPEPRL-------------TRKRWSRNTRVA 437

Query: 388 DKLPLLEEISIPSVSELTNSGIRFSPGNGISAVAFDTKAAKFCLPTITLDVNSEVVLRNL 447
           DK     +  I  V+EL  +GI+F             K     +P + +   ++ +  NL
Sbjct: 438 DK---RRQQLIHCVTELKEAGIKFRRRKTDRFWDMQFKNGYLEIPRLLIHDGTKSLFLNL 497

Query: 448 VAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGIIRNHLKSNAEVTKLWNGMSKS 507
           +A+E          T +I  M+ +ID+ EDV  L   GII + L S++EV  L+N + + 
Sbjct: 498 IAFEQCHIDSSNDITSYIIFMDNLIDSHEDVSYLHYCGIIEHWLGSDSEVADLFNRLCQE 525

Query: 508 IK-STKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGSWPFLTLLATILLLALTALQA 567
           +   T+  +L +   +VN+YY  +W    + L  KY    W  ++  A ++LL LT  Q+
Sbjct: 558 VVFDTEDSYLSRLSIEVNRYYDHKWNAWRATLKHKYFNNPWAIVSFCAAVILLVLTFSQS 525

BLAST of Carg23127 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 132.5 bits (332), Expect = 1.1e-30
Identity = 132/541 (24.40%), Postives = 223/541 (41.22%), Query Frame = 0

Query: 29  QWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPKPLKAIDPNSYTPQEVSIGPYHYWREELH 88
           +WVI I+  +E     +  ++  K+C++ VP  L+  D  SY PQ VSIGPYH+ +  L 
Sbjct: 66  EWVISIKDKMEKALSYDATNSWDKLCIYRVPFYLQENDKKSYLPQTVSIGPYHHGKVHLR 125

Query: 89  EMRRHKIAAAKRAQNRLESLKFQDLVDKITKYEQKTRECYHKYLNLKGET-LAWMLAMDA 148
            M RHK  A      R +    +  +D + + E++ R CY   +++K       ML +D 
Sbjct: 126 PMERHKWRAVNMIMARTKH-NIEMYIDAMKELEEEARACYQGPIDMKNSNEFTEMLVLDG 185

Query: 149 SFLLEVLQ-TCAITKKYSLFRTSSKLSCSVEYDGRKPTDNAILRDIVMLENQVPLFILRN 208
            F+LE+ + T    +K    R     +       ++   ++I RD++MLENQ+PLF+L  
Sbjct: 186 CFVLELFKGTIQGFQKIGYARNDPVFA-------KRGLMHSIQRDMIMLENQLPLFVLDR 245

Query: 209 MLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKKEKDSQALVPECFHLLDFLYRMITPIL 268
           +L  Q        ++A + +  ++ L P                                
Sbjct: 246 LLGLQTGTPNQTGIVAEVAVRFFKTLMP-------------------------------- 305

Query: 269 ANPSEIMEDDHTHKEPTKQHVERNFERSFYPVRELGKQIWKILSKLNSGPAHLYRIIVGF 328
              SE++    T  E +    E++         ELG          ++G  H        
Sbjct: 306 --TSEVL----TKSERSLDSQEKS--------DELG----------DNGGLHC------- 365

Query: 329 RPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLITGKEEDDDDAPEEGSSRTHGKDKLPL 388
             L V  +                       SLI   E  +   P E  S    + +L  
Sbjct: 366 --LDVFHR-----------------------SLIQSSETTNQGTPYEDMSMVEKQQQL-- 425

Query: 389 LEEISIPSVSELTNSGIRFSPGNGISAVAFDTKAAKFCLPTITLDVNSEVVLRNLVAYEA 448
                I  V+EL  +G+ F           + K     +P + +   ++ +  NL+A+E 
Sbjct: 426 -----IHCVTELRGAGVNFMRKETGQLWDIEFKNGYLKIPKLLIHDGTKSLFSNLIAFEQ 485

Query: 449 SKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGIIRNHLKSNAEVTKLWNGMSKS-IKST 508
                    T +I  M+ +I++ +DV  L   GII + L S++EV  L+N + K  I   
Sbjct: 486 CHTQSSNNITSYIIFMDNLINSSQDVSYLHHDGIIEHWLGSDSEVADLFNRLCKEVIFDP 503

Query: 509 KVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGSWPFLTLLATILLLALTALQAFCSVY 567
           K  +L +   +VN+YYS +W    + L +KY    W + +  A ++LL LT  Q+F +VY
Sbjct: 546 KDGYLSQLSREVNRYYSRKWNSLKATLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFAVY 503

BLAST of Carg23127 vs. TAIR 10
Match: AT3G50140.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 127.9 bits (320), Expect = 2.7e-29
Identity = 130/549 (23.68%), Postives = 221/549 (40.26%), Query Frame = 0

Query: 29  QWVIQIRQSLEDVGEIEEEDNEVKVCVFNVPKPLKAIDPNSYTPQEVSIGPYHYWREELH 88
           +WVI I+  +E V       +  K+C++ VP  LK  D NSY PQ VS+GPYH+  E L 
Sbjct: 89  EWVIWIKDKMEQVMRDAATTSWDKICIYRVPLSLKKSDKNSYFPQAVSLGPYHHGDEHLR 148

Query: 89  EMRRHKIAAAKRAQNRLESLKFQDLVDKITKYEQKTRECYHKYLNLKGETLAWMLAMDAS 148
            M  HK  A      R +    +  +D + + E++ R CY   + L       ML +D  
Sbjct: 149 PMDYHKWRAVNMVMKRTKQ-GIEMYIDAMKELEERARACYEGPIGLSSNKFTQMLVLDGC 208

Query: 149 FLLEVLQTCAITKKYSLFRTSSKLSCSVEYDGRKP------TDNAILRDIVMLENQVPLF 208
           F+L+            LFR + +    + YD   P      + ++I RD++MLENQ+PLF
Sbjct: 209 FVLD------------LFRGAYEGFSKLGYDRNDPVFAMRGSMHSIRRDMLMLENQLPLF 268

Query: 209 ILRNMLAYQYTFVEPAQVLASMLLALYEYLSPFKGKKKEKDSQALVPECFHLLDFLYRMI 268
           +L  +L  Q        ++A + +  +  L P                            
Sbjct: 269 VLNRLLELQLGTQYQTGLVAQLAVRFFNPLMP---------------------------- 328

Query: 269 TPILANPSEIMEDDHTHKEPTK-QHVERNFERSFYPVRELGKQIWKILSKLNSGPAHLYR 328
                          T+   TK ++ + N  + F P+ +  K+    L            
Sbjct: 329 ---------------TYMSSTKIENSQENNNKFFNPIADKEKEELHCLD----------- 388

Query: 329 IIVGFRPLQVIFKLPWTIISKLPGIGILLKPLEHLCSLITGKEEDDDDAPEEGSSRTHGK 388
                     +F+              LL+P     SL           P+   SR+   
Sbjct: 389 ----------VFRRS------------LLQP-----SL----------KPDPRLSRSRWS 448

Query: 389 DKLPLL----EEISIPSVSELTNSGIRFSPGNGISAVAFDTKAAKFCLPTITLDVNSEVV 448
            K PL+    ++  +  V+EL  +GI+F             K     +P + +   ++ +
Sbjct: 449 RK-PLVADKRQQQLLHCVTELREAGIKFKRRKSDRFWDIQFKNGCLEIPKLLIHDGTKSL 508

Query: 449 LRNLVAYEASKPSGGLVFTRFIDLMNGIIDTEEDVELLTAAGIIRNHLKSNAEVTKLWNG 508
             NL+AYE          T +I  M+ +ID+ ED+  L    II + L +++EV  ++N 
Sbjct: 509 FSNLIAYEQCHIDSTNDITSYIIFMDNLIDSAEDIRYLHYYDIIEHWLGNDSEVADVFNR 532

Query: 509 MSKSIK-STKVPFLDKAIEDVNKYYSSRWKVKASKLVKKYVFGSWPFLTLLATILLLALT 566
           + + +    +  +L +    V++YY+ +W V  + L  KY    W + +  A ++LL LT
Sbjct: 569 LCQEVAFDLENTYLSELSNKVDRYYNRKWNVLKATLKHKYFSNPWAYFSFFAAVILLLLT 532