Carg23067 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23067
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	nicotinamidase 1-like
Location	Carg_Chr11: 9408833 .. 9413090 (+)
RNA-Seq Expression	Carg23067
Synteny	Carg23067

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAACAGAAGATTTCAAAGATCGTGACGTTTTTTCTTTTGACTAACTTGAACTGATCTAATCTCCTTAATATCAACTCCATTTTGGAATAATTAAACTCTGATCTCTCTTATCTTCGGCTGCCGCTACATCTTTTCTCTGATTTCCCAAAAATGGTTTCCACCGTCATTGATCTTCTCAAAAACGAGATTCCGCTGGAGCAGGAATCTGTGGTTTTGGCTGAAGATACTGTTAATGGCCTTGTTCTTGTTGATATCATCAATGGCTTCTGCACTGTTGGCGCTGGAAATTTGGTAAGTGTTTTGCACTTGGGTTTGTTATATTGCACACTATCTGTTTGATGAAATGCCTCTGCGGTTTTTCCTGAAAATTTGGCTGTTTTCTTTCTTGTTGTTATTGCACACAATCTGTTTGATGTTATGCTTCTGTAGATTTACCGAAGAATTTGGCAAGTTTTCGTTAATTTGTTGCATCCTTGGAATATATTCTGTTTGATGTTATCATTCTTCTGCGTCTTAACTGGCTAGTTTATGGGTTTTTTCTACATGTAGATGTATGCGTAGAAGATTCGAACATTTGATCTATTAGTTGATGAAGCTTAAGTTTACTGTTCATTGAACTTTATTGAACTTCTCACCTTTACATATTTTGATGCTATACTTTTGCTCGTCGACACATGGGTGCCAACCAAGACCCCCTGATAAGAATTCTTACAACTTCGGACTAGATTCACCCATTTGAATTAGTTGATTTTACAAAATCGATCAAGCAGGCTGGTTGGGAAGGGATTTTTAGTGGAGAAAAGAATCGATGAGAGATAGATTTGACTATTTTGTCAGAATGAATGTGTAGATATATAGCATGCTTTGGAAGAGATTGACCCTGAGAGTCAGTCCAGTAAAAAAAGGAGAGGGCCCGTTGTCTAGACTGCATTTTGGGAGTTTGAACACCATCCCATTTTCATGTTCGGTCTAATAACGGGTCTCACTCTCCATATTCTTATTCACTTTAGTTGGACTAAAATTTACACAGTCACACAGTCTTACTTCTCAAGTTCTAACCAAAAGATTCTATTCAATTTGGACTAAAAGCAAATATAGTTTTACTTTTAGATTCTAGAGAGAATCCTCGTTCTAATAAAAGTAATATTAGCAACTTGAGCGAGGTTCTACTTGTTGGGCATAGACCTACCTTTGATGGAAATGTTAACAGTTTGAATCTCACCTTCACCTTCACCTCTCTTGTTAAACTAAGTAGAAATAAAATTGATGTTTTTCTCTTCCGTGTTTCTTTTTCTGTAATAGGCTAGAAAATTGGTGGAATGAGAGTATTAGGTTATGGAGTCTGCTGCTTATTTATAACTTCACTCAATGGTTATACATACTAAAACTATATCTATGGTAAATGACATATAAAGTAAATGGTAGATAGTTATATACGTTAATTGTGGCTATATCTTGTAAAATTACATATAGTAAACTGAACCATATATGTAACTCATTAGGCAGTGCTGTAAAAATGTACTTTCTATATTCTTTGTATGACATATAAAGTAAATGGTAGATAGTTATTTACCGTAGATGATGACAATATCTGGTAAAATTATATATATAGTAAACTCAACCATATATGTACCCGATTAGGCCGATGCTTTGAAAATGTATTTTCTCTTCTAGTACATACTTGAAGTCATCGATATAAATCTCTAGTTAATATTTCTCATTGCATTTTCTCTCTACTGTTCTTGTGTTCATTTAGATCGAGTGTGTATTGGTGTGCAATCCTGTGTGATCCCTCATCAGTTGGGGAGGAGAACGAAACATCCTTTGTAAGGGTGTGGAAACCTCTCTCTAACATACGCGTCGCGTTTTAAAGCCTTACGGGGAAGCCCAAAAGTAAAAACTCAAAGAAGACAATACCTGCTAGCGGTGGACTAGTTACAGATCCTAACAAAGGAAAGAACGTAAATTAGAACGTTGTTGTTTATGGGATGTTTTTGTTGGCAGGCACCAAGAGAACCAAACAGGCAGATCTCTGAAATGGTAGAGGAATCAGCAAGGATTGCAAGGATTTTCTGTGAGAAGAAATGGCCTGTAATGGCATTTCTTGACTCTCACCAACCAAACAAGCCTGAGGAACCATATCCTCCTCACTGTATTGCTGGTTCTGATGAATCCAACTTGGTTCCAGGCATGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTTTTTCCCTTATATCAAACCAAACAGATTGCATCAAAAACAAGTAAATGATCAGAAAATTTTAGATGAATATCAGAATGATTTGAAGAAACCCATGGTTTTAGCAAATCTAGTGTATGTTTATACGTCGAAAATATGGGAACTGGACCTTAGAACCTATGACGACTTTTTAAGTAATAATATATATTTTAACGAATGAAATTACGCTCAAGTTTGACACAATTAGCCAAATTCATATAATTAAAACGTGTCACCGTTTAACTAAATCATTAGTTAGAGTAAGCTGAGAGCTTGAAAATTAAGAAAGTATGTACTATTTTCATTCCAATCTTCCCAATTTACCTGTGATCTTTTAGGTGGAAGAATCTTAGAAACAAATTTTGCATTTTTTCTTTATATATTGTTAAATTATGAAAATTACAATAATTGTAATGGGTCAAAACAAGAAGGGGAGTGGTCTAGTGGTAGAATAGTACCCTGCCACGTTTCAGACCCGTGTTCGACTCTCGGCTGGTGCATCTGATGTTTTTTCATTTATTTTTTTTTTTTTGATAAGTTTCCCTCCTTTTATCATTAATGTTTTGAAACGTGGACTGAGTGGATGAAAATTGTAGCACTGCGATGGGTGGAGAAGGAGCCGAACGTGACGATCAGGCGAAAGGACTGCTTCGACGGCTATATTGGCTCCTTCCAGCCTGATGGCTCCAACGTCTTTGTGGATTGGATCAAACACAATCAAATTAAATCGGTATGAATCTTCTTCCACTTTTCATTTCTTTTCAATGTCTTTGTGGATTGGATCAAACACAATCAAATCGAATCGAATCTGTATGAATCTTCTTCCACTTTTCATTTTTTTTCAATGTCTTCTTTGTGGATTGGATCAAATCGAATCTGTATGAATCTTCTTCCACTTTTCATTTCTTTTCAATGTCTTCTTTGTGGATTGGATCAAATCGAATCTGTATGAATCTTCTTCCACTTTTCATTTCTTTTCAATGTCTTCTTTGTGGATTGGATCAAATCGAATCTGTATGAATCTTCTTCCACTTTTCATTTCTTTTCAATGTCTTCTTTGTGGATTGGATCAAATCGAATCTGTATGAATCTTCTTCCACTTTTCATTTCTTTTCAATGTCTTCTTTGTGGATTGGATCAAATCGAATCTGTATGAATCTTCTTCCACTTTTCATTTCTTTTCAATGTCTTTGGGATTGGATCAAACACGATATGATCCAGCAAGAGCTGTTGATTTGGAACTCAAACCTTTTCTTTTCTGTGGATTAAGCTTTTCGTGGTCGGAATTTGTACGGACATTTGCGTATTGGATTTCGTGTGCTCCACTATGTCCGCCAAAAATCTAGGGCTTCTCAGTCCGTTAAACGACGTCGTTCTGTACTCTCAGGCCTGTGCTACCTTTGATGTTCCTCTCCATGTTGCTACAAACACCAAAGGAGCTCTTCCTCATCCTCAGGTAATACATTTTTCGTTCTTTTTCTCTCTTTTTCTTCCTAATTGACTAATTAAAGAAAATAATTGAAATTATGTAATAATAAGAAAAAAGAATTTCGAGAAAAAAATTAAAAAAAGAGAGAATATATATTTTACGTGGAGTAGGATATTTGAAAAGATTATGTTTATGCGTAGAACGCTCGCATTTTGGGTCATGAAGTACTTCTAATTATTTAAGTGCACCATTTGGTTATAGTTTAATATACTTTTAACACTTTGGGTTATGAACGGTATGGCAGGAGTTCATGCACCATGTCGGCTTGTACATGGCGAAGGAAAGAGGTGCTGTTATAGCCAACAAAATATCACTAATAGAAACCACCGTTAAAGCCAACATACCCACCTTGCTTAATTAGTTGATCTTTGAATCGAATAAATAAATGGGAAGATGAACTTACCAAATAGAAAGTTTTTAAACTCGTGAGGAATACTTTAAATTAATCGAACTAAAGGAAGATATAGTAGTCTTCTTGGTTACCGTCATGTAAATTCTAAAATAAATTATTTTGTTACCTT

mRNA sequence

CAACAGAAGATTTCAAAGATCGTGACGTTTTTTCTTTTGACTAACTTGAACTGATCTAATCTCCTTAATATCAACTCCATTTTGGAATAATTAAACTCTGATCTCTCTTATCTTCGGCTGCCGCTACATCTTTTCTCTGATTTCCCAAAAATGGTTTCCACCGTCATTGATCTTCTCAAAAACGAGATTCCGCTGGAGCAGGAATCTGTGGTTTTGGCTGAAGATACTGTTAATGGCCTTGTTCTTGTTGATATCATCAATGGCTTCTGCACTGTTGGCGCTGGAAATTTGGCACCAAGAGAACCAAACAGGCAGATCTCTGAAATGGTAGAGGAATCAGCAAGGATTGCAAGGATTTTCTGTGAGAAGAAATGGCCTGTAATGGCATTTCTTGACTCTCACCAACCAAACAAGCCTGAGGAACCATATCCTCCTCACTGTATTGCTGGTTCTGATGAATCCAACTTGGTTCCAGCACTGCGATGGGTGGAGAAGGAGCCGAACGTGACGATCAGGCGAAAGGACTGCTTCGACGGCTATATTGGCTCCTTCCAGCCTGATGGCTCCAACGTCTTTGTGGATTGGATCAAACACAATCAAATTAAATCGCTTTTCGTGGTCGGAATTTGTACGGACATTTGCGTATTGGATTTCGTGTGCTCCACTATGTCCGCCAAAAATCTAGGGCTTCTCAGTCCGTTAAACGACGTCGTTCTGTACTCTCAGGCCTGTGCTACCTTTGATGTTCCTCTCCATGTTGCTACAAACACCAAAGGAGCTCTTCCTCATCCTCAGGAGTTCATGCACCATGTCGGCTTGTACATGGCGAAGGAAAGAGGTGCTGTTATAGCCAACAAAATATCACTAATAGAAACCACCGTTAAAGCCAACATACCCACCTTGCTTAATTAGTTGATCTTTGAATCGAATAAATAAATGGGAAGATGAACTTACCAAATAGAAAGTTTTTAAACTCGTGAGGAATACTTTAAATTAATCGAACTAAAGGAAGATATAGTAGTCTTCTTGGTTACCGTCATGTAAATTCTAAAATAAATTATTTTGTTACCTT

Coding sequence (CDS)

ATGGTTTCCACCGTCATTGATCTTCTCAAAAACGAGATTCCGCTGGAGCAGGAATCTGTGGTTTTGGCTGAAGATACTGTTAATGGCCTTGTTCTTGTTGATATCATCAATGGCTTCTGCACTGTTGGCGCTGGAAATTTGGCACCAAGAGAACCAAACAGGCAGATCTCTGAAATGGTAGAGGAATCAGCAAGGATTGCAAGGATTTTCTGTGAGAAGAAATGGCCTGTAATGGCATTTCTTGACTCTCACCAACCAAACAAGCCTGAGGAACCATATCCTCCTCACTGTATTGCTGGTTCTGATGAATCCAACTTGGTTCCAGCACTGCGATGGGTGGAGAAGGAGCCGAACGTGACGATCAGGCGAAAGGACTGCTTCGACGGCTATATTGGCTCCTTCCAGCCTGATGGCTCCAACGTCTTTGTGGATTGGATCAAACACAATCAAATTAAATCGCTTTTCGTGGTCGGAATTTGTACGGACATTTGCGTATTGGATTTCGTGTGCTCCACTATGTCCGCCAAAAATCTAGGGCTTCTCAGTCCGTTAAACGACGTCGTTCTGTACTCTCAGGCCTGTGCTACCTTTGATGTTCCTCTCCATGTTGCTACAAACACCAAAGGAGCTCTTCCTCATCCTCAGGAGTTCATGCACCATGTCGGCTTGTACATGGCGAAGGAAAGAGGTGCTGTTATAGCCAACAAAATATCACTAATAGAAACCACCGTTAAAGCCAACATACCCACCTTGCTTAATTAG

Protein sequence

MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMVEESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVTIRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGLLSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLIETTVKANIPTLLN

Homology

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BLAST of Carg23067 vs. NCBI nr
Match: KAG7022528.1 (Nicotinamidase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 518.8 bits (1335), Expect = 2.5e-143
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 0


Query: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60
           MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV
Sbjct: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60

Query: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120
           EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT
Sbjct: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120

Query: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180
           IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL
Sbjct: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180

Query: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI 240
           LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI
Sbjct: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI 240

Query: 241 ETTVKANIPTLLN 254
           ETTVKANIPTLLN
Sbjct: 241 ETTVKANIPTLLN 253

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BLAST of Carg23067 vs. NCBI nr
Match: KAG6588744.1 (Nicotinamidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 500.7 bits (1288), Expect = 7.2e-138
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0


Query: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60
           MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV
Sbjct: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60

Query: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120
           EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT
Sbjct: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120

Query: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180
           IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL
Sbjct: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180

Query: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI 240
           LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI
Sbjct: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI 240

Query: 241 ETT 244
           ETT
Sbjct: 241 ETT 243

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BLAST of Carg23067 vs. NCBI nr
Match: XP_022927789.1 (nicotinamidase 1-like [Cucurbita moschata])

HSP 1 Score: 498.0 bits (1281), Expect = 4.6e-137
Identity = 242/243 (99.59%), Postives = 242/243 (99.59%), Query Frame = 0


Query: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60
           MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV
Sbjct: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60

Query: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120
           EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT
Sbjct: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120

Query: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180
           IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL
Sbjct: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180

Query: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI 240
           LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI
Sbjct: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI 240

Query: 241 ETT 244
            TT
Sbjct: 241 GTT 243

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BLAST of Carg23067 vs. NCBI nr
Match: XP_022988899.1 (nicotinamidase 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 496.1 bits (1276), Expect = 1.8e-136
Identity = 241/243 (99.18%), Postives = 241/243 (99.18%), Query Frame = 0


Query: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60
           MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV
Sbjct: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60

Query: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120
           EES RIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT
Sbjct: 61  EESTRIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120

Query: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180
           IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL
Sbjct: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180

Query: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI 240
           LSPLNDVVLYSQACATFDVPLHVATNTK ALPHPQEFMHHVGLYMAKERGAVIANKISLI
Sbjct: 181 LSPLNDVVLYSQACATFDVPLHVATNTKRALPHPQEFMHHVGLYMAKERGAVIANKISLI 240

Query: 241 ETT 244
           ETT
Sbjct: 241 ETT 243

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BLAST of Carg23067 vs. NCBI nr
Match: XP_022988898.1 (nicotinamidase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 480.7 bits (1236), Expect = 7.7e-132
Identity = 233/242 (96.28%), Postives = 234/242 (96.69%), Query Frame = 0


Query: 2   VSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMVE 61
           V   I  + NEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMVE
Sbjct: 17  VGKEISSITNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMVE 76

Query: 62  ESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVTI 121
           ES RIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVTI
Sbjct: 77  ESTRIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVTI 136

Query: 122 RRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGLL 181
           RRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGLL
Sbjct: 137 RRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGLL 196

Query: 182 SPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLIE 241
           SPLNDVVLYSQACATFDVPLHVATNTK ALPHPQEFMHHVGLYMAKERGAVIANKISLIE
Sbjct: 197 SPLNDVVLYSQACATFDVPLHVATNTKRALPHPQEFMHHVGLYMAKERGAVIANKISLIE 256

Query: 242 TT 244
           TT
Sbjct: 257 TT 258

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BLAST of Carg23067 vs. ExPASy Swiss-Prot
Match: Q8S8F9 (Nicotinamidase 1 OS=Arabidopsis thaliana OX=3702 GN=NIC1 PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 6.8e-91
Identity = 149/236 (63.14%), Postives = 193/236 (81.78%), Query Frame = 0


Query: 4   TVIDLLKNEIPL-EQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMVEE 63
           T+ D LK +IP+ E+E ++L  D+  GLV+VD++NGFCT+G+GN+AP + N QIS+MVEE
Sbjct: 6   TIFDQLKKQIPVDEEEPLILNRDSSVGLVIVDVVNGFCTIGSGNMAPTKHNEQISKMVEE 65

Query: 64  SARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVTIR 123
           SA++AR FC++KWPV+AF+DSH P+ PE PYPPHCI G++ES LVPAL+W+E E   T+R
Sbjct: 66  SAKLAREFCDRKWPVLAFIDSHHPDIPERPYPPHCIIGTEESELVPALKWLESEDCATLR 125

Query: 124 RKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGLLS 183
           RKDC +G++GS + DGSNVFVDW+K  QIK + VVGICTDICV DFV + +SA+N G+LS
Sbjct: 126 RKDCINGFVGSMESDGSNVFVDWVKEKQIKVIVVVGICTDICVFDFVATALSARNHGVLS 185

Query: 184 PLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKIS 239
           P+ DVV+YS+ CATFD+PLHVA + KGA  HPQE MHHVGLYMAK RGA + +KIS
Sbjct: 186 PVEDVVVYSRGCATFDLPLHVAKDIKGAQAHPQELMHHVGLYMAKGRGAQVVSKIS 241

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BLAST of Carg23067 vs. ExPASy Swiss-Prot
Match: O32091 (Uncharacterized isochorismatase family protein PncA OS=Bacillus subtilis (strain 168) OX=224308 GN=pncA PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.9e-08
Identity = 57/206 (27.67%), Postives = 88/206 (42.72%), Query Frame = 0


Query: 30  LVLVDIINGFCTVGAGNLAPREPNRQISEMVEESARIARIFCEKKWPVMAFLDSHQPNKP 89
           L+ +D  N F     G L   EP R I E +     + + F      V+  +DSH     
Sbjct: 5   LICIDYTNDF-VASDGKLTCGEPGRMIEEAI---VNLTKEFITNGDYVVLAVDSHDEGDQ 64

Query: 90  EEP----YPPHCIAGSDESN----LVPALRWVEKEPNVTIRRKDCFDGYIGSFQPDGSNV 149
             P    +PPH I G++  +    L+P  +  E EPNV    K  +  + G+        
Sbjct: 65  YHPETRLFPPHNIKGTEGKDLYGKLLPLYQKHEHEPNVYYMEKTRYSAFAGT-------D 124

Query: 150 FVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGLLSPLNDVVLYSQACATFDVPL 209
               ++  QI  L + G+CTDICVL    + + A N G       +V++ QA A+F+   
Sbjct: 125 LELKLRERQIGELHLAGVCTDICVLH---TAVDAYNKGF-----RIVVHKQAVASFNQEG 183

Query: 210 HVATNTKGALPHPQEFMHHVGLYMAK 228
           H       AL H   F + +G  +A+
Sbjct: 185 HA-----WALSH---FANSIGAQVAE 183

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BLAST of Carg23067 vs. ExPASy TrEMBL
Match: A0A6J1EIZ6 (nicotinamidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434580 PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.2e-137
Identity = 242/243 (99.59%), Postives = 242/243 (99.59%), Query Frame = 0


Query: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60
           MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV
Sbjct: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60

Query: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120
           EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT
Sbjct: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120

Query: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180
           IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL
Sbjct: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180

Query: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI 240
           LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI
Sbjct: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI 240

Query: 241 ETT 244
            TT
Sbjct: 241 GTT 243

[Close]

BLAST of Carg23067 vs. ExPASy TrEMBL
Match: A0A6J1JMW0 (nicotinamidase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486116 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 8.5e-137
Identity = 241/243 (99.18%), Postives = 241/243 (99.18%), Query Frame = 0


Query: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60
           MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV
Sbjct: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60

Query: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120
           EES RIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT
Sbjct: 61  EESTRIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120

Query: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180
           IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL
Sbjct: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180

Query: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI 240
           LSPLNDVVLYSQACATFDVPLHVATNTK ALPHPQEFMHHVGLYMAKERGAVIANKISLI
Sbjct: 181 LSPLNDVVLYSQACATFDVPLHVATNTKRALPHPQEFMHHVGLYMAKERGAVIANKISLI 240

Query: 241 ETT 244
           ETT
Sbjct: 241 ETT 243

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BLAST of Carg23067 vs. ExPASy TrEMBL
Match: A0A6J1JNN5 (nicotinamidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486116 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 3.7e-132
Identity = 233/242 (96.28%), Postives = 234/242 (96.69%), Query Frame = 0


Query: 2   VSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMVE 61
           V   I  + NEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMVE
Sbjct: 17  VGKEISSITNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMVE 76

Query: 62  ESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVTI 121
           ES RIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVTI
Sbjct: 77  ESTRIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVTI 136

Query: 122 RRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGLL 181
           RRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGLL
Sbjct: 137 RRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGLL 196

Query: 182 SPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLIE 241
           SPLNDVVLYSQACATFDVPLHVATNTK ALPHPQEFMHHVGLYMAKERGAVIANKISLIE
Sbjct: 197 SPLNDVVLYSQACATFDVPLHVATNTKRALPHPQEFMHHVGLYMAKERGAVIANKISLIE 256

Query: 242 TT 244
           TT
Sbjct: 257 TT 258

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BLAST of Carg23067 vs. ExPASy TrEMBL
Match: A0A6J1C7M7 (nicotinamidase 1-like OS=Momordica charantia OX=3673 GN=LOC111008178 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 4.6e-122
Identity = 215/247 (87.04%), Postives = 230/247 (93.12%), Query Frame = 0


Query: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60
           MVS+ IDLLK EIPLEQESVVLAED VNGLVLVDIINGFCTVGAGNLAPREPNRQIS MV
Sbjct: 1   MVSSAIDLLKKEIPLEQESVVLAEDAVNGLVLVDIINGFCTVGAGNLAPREPNRQISGMV 60

Query: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120
           EESARIAR FCEKKWP++AFLDSHQPNKPEEPYPPHCI+GS ESNLVPAL W+EKE NVT
Sbjct: 61  EESARIARAFCEKKWPILAFLDSHQPNKPEEPYPPHCISGSHESNLVPALEWLEKEANVT 120

Query: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180
           +RRKDCFDGY+GSFQPDGSNVFVDWIK+N+I+SL VVGICTDICVLDFVCSTMSA+NLG 
Sbjct: 121 MRRKDCFDGYLGSFQPDGSNVFVDWIKNNRIRSLLVVGICTDICVLDFVCSTMSARNLGF 180

Query: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISLI 240
           LSPLNDVVLYSQACATFDVPLHVAT+TK AL HPQE MHH+GLYMAKERGA+IANKIS I
Sbjct: 181 LSPLNDVVLYSQACATFDVPLHVATSTKAALAHPQELMHHIGLYMAKERGAIIANKIS-I 240

Query: 241 ETTVKAN 248
            T  +AN
Sbjct: 241 GTAEEAN 246

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BLAST of Carg23067 vs. ExPASy TrEMBL
Match: A0A0A0K628 (Isochorismatase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G065110 PE=3 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 1.7e-121
Identity = 213/239 (89.12%), Postives = 224/239 (93.72%), Query Frame = 0


Query: 1   MVSTVIDLLKNEIPLEQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMV 60
           MVS+ IDLLK EIPLEQESVVLAED VNGLVLVDII+GFCTVGAGNLAPREPNRQISEMV
Sbjct: 1   MVSSAIDLLKKEIPLEQESVVLAEDAVNGLVLVDIIHGFCTVGAGNLAPREPNRQISEMV 60

Query: 61  EESARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVT 120
           EESARIARIFCEKKWPVMAF+DSHQPNKPE+PYPPHCI GS ESNLVPAL+WVEKE NVT
Sbjct: 61  EESARIARIFCEKKWPVMAFIDSHQPNKPEKPYPPHCIVGSHESNLVPALQWVEKEANVT 120

Query: 121 IRRKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGL 180
           IRRKDCFDGY+GSF PDGSN+FVDWIK+N IKSL VVGICTDICVLDFVCSTMSAKNLG 
Sbjct: 121 IRRKDCFDGYMGSFLPDGSNLFVDWIKNNHIKSLLVVGICTDICVLDFVCSTMSAKNLGF 180

Query: 181 LSPLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKISL 240
           L PLNDVVLYS++CATFD PLHVA NT  ALPHPQEFMHHVGLYMAKERGA+IANKISL
Sbjct: 181 LHPLNDVVLYSRSCATFDNPLHVAKNTNSALPHPQEFMHHVGLYMAKERGAIIANKISL 239

[Close]

BLAST of Carg23067 vs. TAIR 10
Match: AT2G22570.1 (nicotinamidase 1 )

HSP 1 Score: 335.1 bits (858), Expect = 4.9e-92
Identity = 149/236 (63.14%), Postives = 193/236 (81.78%), Query Frame = 0


Query: 4   TVIDLLKNEIPL-EQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMVEE 63
           T+ D LK +IP+ E+E ++L  D+  GLV+VD++NGFCT+G+GN+AP + N QIS+MVEE
Sbjct: 6   TIFDQLKKQIPVDEEEPLILNRDSSVGLVIVDVVNGFCTIGSGNMAPTKHNEQISKMVEE 65

Query: 64  SARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVTIR 123
           SA++AR FC++KWPV+AF+DSH P+ PE PYPPHCI G++ES LVPAL+W+E E   T+R
Sbjct: 66  SAKLAREFCDRKWPVLAFIDSHHPDIPERPYPPHCIIGTEESELVPALKWLESEDCATLR 125

Query: 124 RKDCFDGYIGSFQPDGSNVFVDWIKHNQIKSLFVVGICTDICVLDFVCSTMSAKNLGLLS 183
           RKDC +G++GS + DGSNVFVDW+K  QIK + VVGICTDICV DFV + +SA+N G+LS
Sbjct: 126 RKDCINGFVGSMESDGSNVFVDWVKEKQIKVIVVVGICTDICVFDFVATALSARNHGVLS 185

Query: 184 PLNDVVLYSQACATFDVPLHVATNTKGALPHPQEFMHHVGLYMAKERGAVIANKIS 239
           P+ DVV+YS+ CATFD+PLHVA + KGA  HPQE MHHVGLYMAK RGA + +KIS
Sbjct: 186 PVEDVVVYSRGCATFDLPLHVAKDIKGAQAHPQELMHHVGLYMAKGRGAQVVSKIS 241

[Close]

BLAST of Carg23067 vs. TAIR 10
Match: AT2G22570.2 (nicotinamidase 1 )

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-55
Identity = 91/150 (60.67%), Postives = 123/150 (82.00%), Query Frame = 0


Query: 4   TVIDLLKNEIPL-EQESVVLAEDTVNGLVLVDIINGFCTVGAGNLAPREPNRQISEMVEE 63
           T+ D LK +IP+ E+E ++L  D+  GLV+VD++NGFCT+G+GN+AP + N QIS+MVEE
Sbjct: 6   TIFDQLKKQIPVDEEEPLILNRDSSVGLVIVDVVNGFCTIGSGNMAPTKHNEQISKMVEE 65

Query: 64  SARIARIFCEKKWPVMAFLDSHQPNKPEEPYPPHCIAGSDESNLVPALRWVEKEPNVTIR 123
           SA++AR FC++KWPV+AF+DSH P+ PE PYPPHCI G++ES LVPAL+W+E E   T+R
Sbjct: 66  SAKLAREFCDRKWPVLAFIDSHHPDIPERPYPPHCIIGTEESELVPALKWLESEDCATLR 125

Query: 124 RKDCFDGYIGSFQPDGSNVFVDWIKHNQIK 153
           RKDC +G++GS + DGSNVFVDW+K  QIK
Sbjct: 126 RKDCINGFVGSMESDGSNVFVDWVKEKQIK 155

The following BLAST results are available for this feature:

BLAST of Carg23067 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022528.1	2.5e-143	100.00	Nicotinamidase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6588744.1	7.2e-138	100.00	Nicotinamidase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022927789.1	4.6e-137	99.59	nicotinamidase 1-like [Cucurbita moschata]	[more]
XP_022988899.1	1.8e-136	99.18	nicotinamidase 1-like isoform X2 [Cucurbita maxima]	[more]
XP_022988898.1	7.7e-132	96.28	nicotinamidase 1-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg23067 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8F9	6.8e-91	63.14	Nicotinamidase 1 OS=Arabidopsis thaliana OX=3702 GN=NIC1 PE=1 SV=1	[more]
O32091	1.9e-08	27.67	Uncharacterized isochorismatase family protein PncA OS=Bacillus subtilis (strain...	[more]

BLAST of Carg23067 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EIZ6	2.2e-137	99.59	nicotinamidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434580 PE=3 SV=1	[more]
A0A6J1JMW0	8.5e-137	99.18	nicotinamidase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486116 PE=...	[more]
A0A6J1JNN5	3.7e-132	96.28	nicotinamidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486116 PE=...	[more]
A0A6J1C7M7	4.6e-122	87.04	nicotinamidase 1-like OS=Momordica charantia OX=3673 GN=LOC111008178 PE=3 SV=1	[more]
A0A0A0K628	1.7e-121	89.12	Isochorismatase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G06...	[more]

BLAST of Carg23067 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22570.1	4.9e-92	63.14	nicotinamidase 1	[more]
AT2G22570.2	1.2e-55	60.67	nicotinamidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000868	Isochorismatase-like	PFAM	PF00857	Isochorismatase	coord: 29..204 e-value: 4.0E-27 score: 95.5
IPR036380	Isochorismatase-like superfamily	GENE3D	3.40.50.850		coord: 28..211 e-value: 3.3E-27 score: 97.8
IPR036380	Isochorismatase-like superfamily	SUPERFAMILY	52499	Isochorismatase-like hydrolases	coord: 30..233
None	No IPR available	PANTHER	PTHR47297:SF1	NICOTINAMIDASE 1	coord: 1..241
None	No IPR available	CDD	cd00431	cysteine_hydrolases	coord: 30..205 e-value: 4.9332E-30 score: 108.125
IPR044717	Nicotinamidase 1	PANTHER	PTHR47297	FAMILY NOT NAMED	coord: 1..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23067-RA	Carg23067-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019365	pyridine nucleotide salvage
molecular_function	GO:0008936	nicotinamidase activity