Carg23029 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23029
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein NRT1/ PTR FAMILY 4.4-like
LocationCarg_Chr01: 5367367 .. 5370450 (-)
RNA-Seq ExpressionCarg23029
SyntenyCarg23029
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGATGCTTTAGAGAGGACAAAAACAAAGAGCAAAGAAATAAATTCCATGTATGAGGACACATTTGTTGATTGGAGAGGCCGACCGTGCACCTCTAACAAACATGGTGGAATGGCTGCCGCTGCCTTTGTTCTTGGTCTGTTTTTTGGTGTTTGAAACAGAGTTTGGTTTCGGTTTTTATGTTTTTAATTTGTGTTTTGTGTGTTTATATGGTTTGTTGTGGCTCAGGGCTTCAAGCATTTGAGATGATGGCTATAGCAGCAGTTGGGAATAATCTCATTACTTACGTTTTCACTCAGATGCATTTTCCTCTCTCTAAATCTGCTAACGTTGTTACCAACTTTGTTGGAACTGTTTTTTTTCTGTCTCTCCTTGGTGGCTTCCTGTCCGATTCTTATCTTGGCAGCTTCCGAACTATGCTCATCTTTGGCTTCCTCGAGCTATCGGTAAATTCCCGTCTAAATCATTGTTTTCAACGGAGCAAACAACAATCATATTGTTATTTTGACCATAAATGCAGAGGATCATAGGGAGATGAGTCCAATAACTAGCCACTAATTTAGCAGTTGATTATGAGTTTATAAATAAGAAATACATCTCTACTAATACGAGACTTTTTCGAAAAACAAAAATAAAAGTCATGAGAACTTATGCTTAAACGGAACAATATCATACCATCTTCAAGAGTTAGTGGTTCCTAACCCAACTCTTCGTAATTTTATTTTTGATCTTTTGAAAAAAAAAAAAAATTATATCTCACGATTCGTTGGGAGGAGAGAATGAAACATCCTTTATAAGGGTGTAGAAACCTTTTCTTAGCATACACGTTTTAAAAACTTTGAGGTGAAGTTCAAAAAGAAAAATCCAAAAAAGATGATATCTACTAGCGGTGGACTTAGGCGGTTATATAGTATCTCTTATTTAATATTTATATGAACTTACAAAAATTTTGAAGTTGGTGATGAAGAAGTGAATCTAGGAAGAGCTGAATAGTGGATTAATATATAATTGCAGGGATTTAGTCTTCTAACAGTGCAAGCCCATTATCCAAGGCTGAGGCCACCAGCATGCGACATGGGAACAGGGCAACAGTGCATGGAGGCGAAAGGGTACGAGGCATTAATGCTGTTCACAGCGCTGTATTTGGTGGCATTGGGAAGTGGGTGTTTAAAACCCAACATAATATCACATGGGGCTGACCAATTTCGAAGACAACACCCAACCCAATTCCAAAAGCTCTCCACTTTCTTCAATTGTGCTTACTTCGCCTTCTGCACTGGCGAGCTCATTGCTTTGACACTCCTTGTTTGGGTCCAAACCCACTATGGCATGGACGTTGGCTTCACCGTATCCACTGCTGCCATGCTTCTTGGCTTGATCTCATTGCTCGCTGGCACCTCCTTCTTTAGAAACAGCCCTCCTCGTGGCACCATCTTCACCCCCATCGCTCAAGTAATTAATTATCCAATTTCAAACATTCACCAAACTTTTTATTTTTTATTTTTTATTTTTTAAATTTTTAATTTTTCCTTTCTGCTGATAGGTTTTTGTAGCTGCATTTGTGAAGAGGAAGCAAATCTGTCCTTCCAATTCGGAGATGCTGCATCGAAGTCAAAACGGCGTCGTTAAACTTCTCCATACCGACAACTTCAGGTGTGTTTCTCAAAATCTAATTATGTTTTTCTTGCTATTTTTGTCTGTTTTTGTTCTTTTAACTATTTATTAGTATTTTTTTTAAAAAAGTTTTAAATAAAAAATTAAATTTTTAATCAAATTTATGAGACAAAACAAATTTATATTAGGATACATAGCCCCATAACTATTTAGAGCAAAAATTGTATAAATCTATGGCAGATATTTCGTAAAAATAGTACACATCTCATAAATTTGGTAAACTGAAACTATATATATCTCATTAGTCAGTTATATTGGATAGTAGGTAGATTATACATATATTTGATAGATTATATTGTAAATTATATATATATATATCCCATTAGTGAGACGTCTAAATATACTTTTTTATTTCTGTACTCTCTGTGTTCTGAAGTGAGCAATAAAATCTTTAGCTAATATTTTTTATAAATAAAATCCTCGATAAAGATTTAGTTTTAAGTATACAATATTTCTTCTTGAATTTTCACTCTTGTTCTTTGTGTTCATCGAGTGTATGCGTGGTTGTGCAGTCTTAACAAGTTTTATAGTCTTATAAAGTGGTTAAGTGTAAACATGATGTATATTTACAGATTCCTTGACAAAGCTTGTATAAAGAGTGAAGAAGGGAGAGTTGGAAGTGAAGAGAGTCCATGGAAGCTGTGCACGGTAACACAAGTGGAACAAGTCAAAATAATTCTCTCACTTATTCCTATATTTGCATGCACCATAATCTTCAACACCATCTTAGCACAGCTCCAAACATTCTCAGTTCAACAAGGAGCTGCCATGAACACATCCCTCACCAACTCCTTCCAAATCCCCCCTGCTTCCCTCCAATCCATCCCTTACATCATTCTCATCTTCCTCGTCCCGCTCTATGAAACCGCCTTCGTCCCCCTTGCCCGCCGCATCACCCACACCAACTCCGGCATTTCCCCTCTCCAACGCGTAGGCACAGGCCTCTTCGTTGCCACATTCTCCATGGTCTCCGCGGCATTAGTTGAGAAAAAGCGTCGAAACTCCTCCATCGTTGGCTCATCCCTCTCGATCCTCTGGATCGCACCGCAATTCATCATTTTCGGCATCTCGGAGCTCTTCACTGCGGTCGGTCTGGTCGAGTTTTTTTACAAGCAATCCGTGGAGGGGATGCAGTCGTTTTTAACAGCCATGACTTATTGCTCCTACTCGTTTGGCTTCTATTTGAGCTCTTTATTGGTGTCGCTTGTTAATAAGATCACTGGTGGGTCTTCGGGTGGTGGGTGGCTTAGCCATAATGACCTAAACAACGACAGATTGGATCTGTTCTATTGGTTGCTGGCAGGTCTCAGCCTTGTCAATTTCTTCAACTATCTCTTTTGGGCCAATAGATATTCTCAAGAACATGAGGCTCAAGACCTTGAAAACCCGACCTCCAAACCTTCCTAA

mRNA sequence

ATGGGGGATGCTTTAGAGAGGACAAAAACAAAGAGCAAAGAAATAAATTCCATGTATGAGGACACATTTGTTGATTGGAGAGGCCGACCGTGCACCTCTAACAAACATGGTGGAATGGCTGCCGCTGCCTTTGTTCTTGGGCTTCAAGCATTTGAGATGATGGCTATAGCAGCAGTTGGGAATAATCTCATTACTTACGTTTTCACTCAGATGCATTTTCCTCTCTCTAAATCTGCTAACGTTGTTACCAACTTTGTTGGAACTGTTTTTTTTCTGTCTCTCCTTGGTGGCTTCCTGTCCGATTCTTATCTTGGCAGCTTCCGAACTATGCTCATCTTTGGCTTCCTCGAGCTATCGGGATTTAGTCTTCTAACAGTGCAAGCCCATTATCCAAGGCTGAGGCCACCAGCATGCGACATGGGAACAGGGCAACAGTGCATGGAGGCGAAAGGGTACGAGGCATTAATGCTGTTCACAGCGCTGTATTTGGTGGCATTGGGAAGTGGGTGTTTAAAACCCAACATAATATCACATGGGGCTGACCAATTTCGAAGACAACACCCAACCCAATTCCAAAAGCTCTCCACTTTCTTCAATTGTGCTTACTTCGCCTTCTGCACTGGCGAGCTCATTGCTTTGACACTCCTTGTTTGGGTCCAAACCCACTATGGCATGGACGTTGGCTTCACCGTATCCACTGCTGCCATGCTTCTTGGCTTGATCTCATTGCTCGCTGGCACCTCCTTCTTTAGAAACAGCCCTCCTCGTGGCACCATCTTCACCCCCATCGCTCAAGTTTTTGTAGCTGCATTTGTGAAGAGGAAGCAAATCTGTCCTTCCAATTCGGAGATGCTGCATCGAAGTCAAAACGGCGTCGTTAAACTTCTCCATACCGACAACTTCAGATTCCTTGACAAAGCTTGTATAAAGAGTGAAGAAGGGAGAGTTGGAAGTGAAGAGAGTCCATGGAAGCTGTGCACGGTAACACAAGTGGAACAAGTCAAAATAATTCTCTCACTTATTCCTATATTTGCATGCACCATAATCTTCAACACCATCTTAGCACAGCTCCAAACATTCTCAGTTCAACAAGGAGCTGCCATGAACACATCCCTCACCAACTCCTTCCAAATCCCCCCTGCTTCCCTCCAATCCATCCCTTACATCATTCTCATCTTCCTCGTCCCGCTCTATGAAACCGCCTTCGTCCCCCTTGCCCGCCGCATCACCCACACCAACTCCGGCATTTCCCCTCTCCAACGCGTAGGCACAGGCCTCTTCGTTGCCACATTCTCCATGGTCTCCGCGGCATTAGTTGAGAAAAAGCGTCGAAACTCCTCCATCGTTGGCTCATCCCTCTCGATCCTCTGGATCGCACCGCAATTCATCATTTTCGGCATCTCGGAGCTCTTCACTGCGGTCGGTCTGGTCGAGTTTTTTTACAAGCAATCCGTGGAGGGGATGCAGTCGTTTTTAACAGCCATGACTTATTGCTCCTACTCGTTTGGCTTCTATTTGAGCTCTTTATTGGTGTCGCTTGTTAATAAGATCACTGGTGGGTCTTCGGGTGGTGGGTGGCTTAGCCATAATGACCTAAACAACGACAGATTGGATCTGTTCTATTGGTTGCTGGCAGGTCTCAGCCTTGTCAATTTCTTCAACTATCTCTTTTGGGCCAATAGATATTCTCAAGAACATGAGGCTCAAGACCTTGAAAACCCGACCTCCAAACCTTCCTAA

Coding sequence (CDS)

ATGGGGGATGCTTTAGAGAGGACAAAAACAAAGAGCAAAGAAATAAATTCCATGTATGAGGACACATTTGTTGATTGGAGAGGCCGACCGTGCACCTCTAACAAACATGGTGGAATGGCTGCCGCTGCCTTTGTTCTTGGGCTTCAAGCATTTGAGATGATGGCTATAGCAGCAGTTGGGAATAATCTCATTACTTACGTTTTCACTCAGATGCATTTTCCTCTCTCTAAATCTGCTAACGTTGTTACCAACTTTGTTGGAACTGTTTTTTTTCTGTCTCTCCTTGGTGGCTTCCTGTCCGATTCTTATCTTGGCAGCTTCCGAACTATGCTCATCTTTGGCTTCCTCGAGCTATCGGGATTTAGTCTTCTAACAGTGCAAGCCCATTATCCAAGGCTGAGGCCACCAGCATGCGACATGGGAACAGGGCAACAGTGCATGGAGGCGAAAGGGTACGAGGCATTAATGCTGTTCACAGCGCTGTATTTGGTGGCATTGGGAAGTGGGTGTTTAAAACCCAACATAATATCACATGGGGCTGACCAATTTCGAAGACAACACCCAACCCAATTCCAAAAGCTCTCCACTTTCTTCAATTGTGCTTACTTCGCCTTCTGCACTGGCGAGCTCATTGCTTTGACACTCCTTGTTTGGGTCCAAACCCACTATGGCATGGACGTTGGCTTCACCGTATCCACTGCTGCCATGCTTCTTGGCTTGATCTCATTGCTCGCTGGCACCTCCTTCTTTAGAAACAGCCCTCCTCGTGGCACCATCTTCACCCCCATCGCTCAAGTTTTTGTAGCTGCATTTGTGAAGAGGAAGCAAATCTGTCCTTCCAATTCGGAGATGCTGCATCGAAGTCAAAACGGCGTCGTTAAACTTCTCCATACCGACAACTTCAGATTCCTTGACAAAGCTTGTATAAAGAGTGAAGAAGGGAGAGTTGGAAGTGAAGAGAGTCCATGGAAGCTGTGCACGGTAACACAAGTGGAACAAGTCAAAATAATTCTCTCACTTATTCCTATATTTGCATGCACCATAATCTTCAACACCATCTTAGCACAGCTCCAAACATTCTCAGTTCAACAAGGAGCTGCCATGAACACATCCCTCACCAACTCCTTCCAAATCCCCCCTGCTTCCCTCCAATCCATCCCTTACATCATTCTCATCTTCCTCGTCCCGCTCTATGAAACCGCCTTCGTCCCCCTTGCCCGCCGCATCACCCACACCAACTCCGGCATTTCCCCTCTCCAACGCGTAGGCACAGGCCTCTTCGTTGCCACATTCTCCATGGTCTCCGCGGCATTAGTTGAGAAAAAGCGTCGAAACTCCTCCATCGTTGGCTCATCCCTCTCGATCCTCTGGATCGCACCGCAATTCATCATTTTCGGCATCTCGGAGCTCTTCACTGCGGTCGGTCTGGTCGAGTTTTTTTACAAGCAATCCGTGGAGGGGATGCAGTCGTTTTTAACAGCCATGACTTATTGCTCCTACTCGTTTGGCTTCTATTTGAGCTCTTTATTGGTGTCGCTTGTTAATAAGATCACTGGTGGGTCTTCGGGTGGTGGGTGGCTTAGCCATAATGACCTAAACAACGACAGATTGGATCTGTTCTATTGGTTGCTGGCAGGTCTCAGCCTTGTCAATTTCTTCAACTATCTCTTTTGGGCCAATAGATATTCTCAAGAACATGAGGCTCAAGACCTTGAAAACCCGACCTCCAAACCTTCCTAA

Protein sequence

MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDNFRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLFYWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS
Homology
BLAST of Carg23029 vs. NCBI nr
Match: KAG7037184.1 (Protein NRT1/ PTR FAMILY 4.4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 579/579 (100.00%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60
           MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120
           NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180
           FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA
Sbjct: 121 FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180

Query: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240
           DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL
Sbjct: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240

Query: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN 300
           ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN
Sbjct: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN 300

Query: 301 FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360
           FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF
Sbjct: 301 FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360

Query: 361 SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420
           SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ
Sbjct: 361 SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420

Query: 421 RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480
           RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF
Sbjct: 421 RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480

Query: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLF 540
           YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLF
Sbjct: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLF 540

Query: 541 YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS 580
           YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS
Sbjct: 541 YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS 579

BLAST of Carg23029 vs. NCBI nr
Match: XP_022932490.1 (protein NRT1/ PTR FAMILY 4.4-like [Cucurbita moschata])

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 576/579 (99.48%), Postives = 578/579 (99.83%), Query Frame = 0

Query: 1   MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60
           MGDALE+TKTKSKEINSMYEDT VDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALEKTKTKSKEINSMYEDTSVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120
           NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180
           FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA
Sbjct: 121 FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180

Query: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240
           DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL
Sbjct: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240

Query: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN 300
           ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN
Sbjct: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN 300

Query: 301 FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360
           FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF
Sbjct: 301 FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360

Query: 361 SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420
           SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ
Sbjct: 361 SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420

Query: 421 RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480
           RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF
Sbjct: 421 RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480

Query: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLF 540
           YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGG+SGGGWLSHNDLNNDRLDLF
Sbjct: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGASGGGWLSHNDLNNDRLDLF 540

Query: 541 YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS 580
           YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS
Sbjct: 541 YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS 579

BLAST of Carg23029 vs. NCBI nr
Match: XP_022973463.1 (protein NRT1/ PTR FAMILY 4.4-like [Cucurbita maxima])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 565/579 (97.58%), Postives = 570/579 (98.45%), Query Frame = 0

Query: 1   MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60
           MGDALE+TKTKSKE+ SM EDT VDWRGRPCTSNKHGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALEKTKTKSKELTSMSEDTSVDWRGRPCTSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120
           NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180
           FSLLTVQAHYPRLRP ACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA
Sbjct: 121 FSLLTVQAHYPRLRPLACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180

Query: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240
           DQFRRQHPTQFQKLSTFFNCAYFAFCTGEL+ALTLLVWVQTHYGMDVGFTVSTAAMLLGL
Sbjct: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELLALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240

Query: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN 300
           ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQI PSNSEMLHRSQNGVVKLLHTD 
Sbjct: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQIYPSNSEMLHRSQNGVVKLLHTDK 300

Query: 301 FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360
           FRFLDKACIKSEEGRVGSEES WKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF
Sbjct: 301 FRFLDKACIKSEEGRVGSEESQWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360

Query: 361 SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420
           SVQQGAAMNT LTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ
Sbjct: 361 SVQQGAAMNTCLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420

Query: 421 RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480
           RVGTGLFVATFSMVSAALVEKKRRNSS+VGSSLSILWIAPQFIIFGISELFTAVGLVEFF
Sbjct: 421 RVGTGLFVATFSMVSAALVEKKRRNSSVVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480

Query: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLF 540
           YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGG+SGGGWLSHNDLNNDRLDLF
Sbjct: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGASGGGWLSHNDLNNDRLDLF 540

Query: 541 YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS 580
           YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS
Sbjct: 541 YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS 579

BLAST of Carg23029 vs. NCBI nr
Match: XP_023523828.1 (protein NRT1/ PTR FAMILY 4.4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 562/579 (97.06%), Postives = 570/579 (98.45%), Query Frame = 0

Query: 1   MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60
           MGDALE+TKTKSK+I SM EDT VDWRGRPCTS+KHGGMAAAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALEKTKTKSKQITSMSEDTSVDWRGRPCTSDKHGGMAAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120
           NNLITYVFTQMHFPLSKSANVVTNFVGTVF LSLLGGFLSDSYLGSFRTMLIFGFLELSG
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFLLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180
           FSL+TVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA
Sbjct: 121 FSLVTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180

Query: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240
           DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL
Sbjct: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240

Query: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN 300
           ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQN VVKLLHTD 
Sbjct: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNAVVKLLHTDK 300

Query: 301 FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360
           F FLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF
Sbjct: 301 FTFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360

Query: 361 SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420
           SVQQGAAMNT LTNSF+IPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ
Sbjct: 361 SVQQGAAMNTCLTNSFKIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420

Query: 421 RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480
           RVGTGLFVATFSMVSAALVEKKRRNSS+VGSSLSILWIAPQFIIFGISELFTAVGLVEFF
Sbjct: 421 RVGTGLFVATFSMVSAALVEKKRRNSSLVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480

Query: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLF 540
           YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITG +SGGGWLSHNDLNNDRLDLF
Sbjct: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGEASGGGWLSHNDLNNDRLDLF 540

Query: 541 YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS 580
           YWLLAGLSLVNFFNYLFWANRYSQEH+AQDLENPTSKPS
Sbjct: 541 YWLLAGLSLVNFFNYLFWANRYSQEHDAQDLENPTSKPS 579

BLAST of Carg23029 vs. NCBI nr
Match: KAG6607544.1 (Protein NRT1/ PTR FAMILY 4.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 979.2 bits (2530), Expect = 1.6e-281
Identity = 500/503 (99.40%), Postives = 502/503 (99.80%), Query Frame = 0

Query: 18  MYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSK 77
           MYEDT VDWRGRPCTS+KHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSK
Sbjct: 1   MYEDTSVDWRGRPCTSDKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSK 60

Query: 78  SANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPA 137
           SANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPA
Sbjct: 61  SANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPA 120

Query: 138 CDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTF 197
           CDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTF
Sbjct: 121 CDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTF 180

Query: 198 FNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRG 257
           FNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRG
Sbjct: 181 FNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRG 240

Query: 258 TIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDNFRFLDKACIKSEEGRVG 317
           TIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDNFRFLDKACIKSEEGRVG
Sbjct: 241 TIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDNFRFLDKACIKSEEGRVG 300

Query: 318 SEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNSFQ 377
           SEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNSFQ
Sbjct: 301 SEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNSFQ 360

Query: 378 IPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTGLFVATFSMVSAA 437
           IPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQR+GTGLFVATFSMVSAA
Sbjct: 361 IPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRIGTGLFVATFSMVSAA 420

Query: 438 LVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTY 497
           LVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTY
Sbjct: 421 LVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTY 480

Query: 498 CSYSFGFYLSSLLVSLVNKITGG 521
           CSYSFGFYLSSLLVSLVNKITGG
Sbjct: 481 CSYSFGFYLSSLLVSLVNKITGG 503

BLAST of Carg23029 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 7.0e-224
Identity = 403/557 (72.35%), Postives = 465/557 (83.48%), Query Frame = 0

Query: 20  EDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSA 79
           +D  VD+RGRPC  +KHGG  AA FVLG QAFEMMAIAAVGNNLITYVF +MHFPLSKSA
Sbjct: 23  DDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSA 82

Query: 80  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPACD 139
           N+VTNF+GTVF LSLLGGFLSDSYLGSFRTML+FG +E+SGF LL+VQAH P LRPP C+
Sbjct: 83  NLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECN 142

Query: 140 M-GTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFF 199
           M  T   C+EA GY+A  L+TAL LVALGSGCLKPNIISHGA+QF+R+     +KLS+FF
Sbjct: 143 MKSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRK---DLRKLSSFF 202

Query: 200 NCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGT 259
           N AYFAF  G+LIALTLLVWVQTH GMDVGF VS A M  G+ISL+AGTSF+RN PP G+
Sbjct: 203 NAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGS 262

Query: 260 IFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVK---LLHTDNFRFLDKACIKSEEGR 319
           IFTPIAQVFVAA  KRKQICPSN  M+H+    +V+   LLH++ FRFLDKACIK+ +G+
Sbjct: 263 IFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIKT-QGK 322

Query: 320 VGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNS 379
             + ESPW+LCT+ QV QVKI+LS+IPIFACTIIFNTILAQLQTFSVQQG++MNT +T +
Sbjct: 323 --AMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKT 382

Query: 380 FQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTGLFVATFSMVS 439
           FQIPPASLQ+IPYIILIF VPLYET FVPLAR++T  +SGISPLQR+GTGLF+ATFSMV+
Sbjct: 383 FQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVA 442

Query: 440 AALVEKKRRNSSIVGS-SLSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFLTA 499
           AALVEKKRR S +  +  LSI WIAPQF+IFG+SE+FTAVGLVEFFYKQS + MQSFLTA
Sbjct: 443 AALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTA 502

Query: 500 MTYCSYSFGFYLSSLLVSLVNKITGGSSGG---GWLSHNDLNNDRLDLFYWLLAGLSLVN 559
           MTYCSYSFGFYLSS+LVS VN++T  +  G   GWL  NDLN DRLD FYWLLA LS +N
Sbjct: 503 MTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFIN 562

Query: 560 FFNYLFWANRYSQEHEA 569
           FFNYLFW+  YS +  A
Sbjct: 563 FFNYLFWSRWYSCDPSA 573

BLAST of Carg23029 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 2.1e-212
Identity = 377/562 (67.08%), Postives = 457/562 (81.32%), Query Frame = 0

Query: 6   ERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLIT 65
           E+ +  ++    + E+  VDWRGRP   NKHGGM AA FVLGLQAFE+M IAAVGNNLIT
Sbjct: 12  EQEEVSNENNWELAEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLIT 71

Query: 66  YVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLT 125
           YV  +MHFPLSK+AN+VTNFVGT+F  +LLGG+LSD++LGSF T++IFGF+ELSGF LL+
Sbjct: 72  YVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLS 131

Query: 126 VQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRR 185
           VQAH P+L+PP C+    Q C EAKG++A++ F ALYLVALGSGC+KPN+I+HGADQF +
Sbjct: 132 VQAHLPQLKPPKCNPLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQ 191

Query: 186 QHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLA 245
            HP Q ++LS++FN AYFAF  GELIALTLLVWVQTH GMD+GF VS AAM +G+ISL++
Sbjct: 192 SHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVS 251

Query: 246 GTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQ---NGVV---KLLHTD 305
           GT +FRN  PR +IFTPIA V VAA +KRK   PS+  MLH      N VV    L HT 
Sbjct: 252 GTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTP 311

Query: 306 NFRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQT 365
            FRFLDKACIK ++    ++ESPW+LCTVTQVEQVK ++SL+PIFA TI+FNTILAQLQT
Sbjct: 312 RFRFLDKACIKIQD--TNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQT 371

Query: 366 FSVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPL 425
           FSVQQG++MNT L+NSF IPPASLQ+IPYI+LIFLVPLY++  VP AR++T  NSGI PL
Sbjct: 372 FSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPL 431

Query: 426 QRVGTGLFVATFSMVSAALVEKKRRNSSIV-GSSLSILWIAPQFIIFGISELFTAVGLVE 485
            R+G GLF++TFSMVSAA++EKKRR+SS++ G  LSI WI PQF+IFGISE+FTAVGL+E
Sbjct: 432 TRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIE 491

Query: 486 FFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGS-SGGGWLSHNDLNNDRL 545
           FFYKQS +GM+SFL A+TYCSYSFGFY SS+LVS+VNKIT  S    GWL  NDLN DRL
Sbjct: 492 FFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRL 551

Query: 546 DLFYWLLAGLSLVNFFNYLFWA 560
           DLFYWLLA LSL+NF +YLFW+
Sbjct: 552 DLFYWLLAVLSLLNFLSYLFWS 571

BLAST of Carg23029 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.8e-115
Identity = 244/580 (42.07%), Postives = 342/580 (58.97%), Query Frame = 0

Query: 23  FVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVV 82
           + DWR R     +HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH   SKSAN V
Sbjct: 13  YADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDV 72

Query: 83  TNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPACDMGT 142
           TNF+GT F L+LLGGFLSD++  +F+  LI   +E  G  +LT+QA  P L PP+CD  T
Sbjct: 73  TNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCDSPT 132

Query: 143 GQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFFNCAY 202
              C E  G +A MLF  LYLVALG G +K ++ SHGA+QF    P   ++ STFFN  Y
Sbjct: 133 ---CEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFN--Y 192

Query: 203 FAFC--TGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGTIF 262
           F FC   G L+A+T +VW++ + G + GF VST A+ + ++  L+G+ F+RN  P G+  
Sbjct: 193 FVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPL 252

Query: 263 TPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDNFRFLDKACIKSEEGRVGSEE 322
           T I +V +AA VK    C S S     S N V  +  + +   + K   K E    G  E
Sbjct: 253 TTILKVLLAASVK---CCSSGS-----SSNAVASMSVSPSNHCVSKG--KKEVESQGELE 312

Query: 323 SPWK------------------------------LCTVTQVEQVKIILSLIPIFACTIIF 382
            P +                               CTV QVE VKI+L ++PIFACTI+ 
Sbjct: 313 KPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIML 372

Query: 383 NTILAQLQTFSVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRIT 442
           N  LAQL TFSVQQ A+MNT +  S +IPPASL   P + ++ L P+Y+   +P AR+ T
Sbjct: 373 NCCLAQLSTFSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKAT 432

Query: 443 HTNSGISPLQRVGTGLFVATFSMVSAALVEKKR----RNSSIVGSS----LSILWIAPQF 502
            T +G++ LQR+G GL ++  +M  AALVE KR    ++S ++ S     ++ LWIA Q+
Sbjct: 433 KTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQY 492

Query: 503 IIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSS 562
           +  G ++LFT  GL+E+F+ ++   M+S  T++++ S + G+YLSS++VS+VN ITG S 
Sbjct: 493 LFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSG 552

BLAST of Carg23029 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 2.6e-109
Identity = 220/550 (40.00%), Postives = 326/550 (59.27%), Query Frame = 0

Query: 23  FVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVV 82
           + DWR +     +HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH  L++S++ V
Sbjct: 15  YADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEV 74

Query: 83  TNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPACDMGT 142
           T F+ T F L+LLGGFL+D++  +F   LI   +E  G  LLT+QA  P L PP C    
Sbjct: 75  TTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSA 134

Query: 143 GQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFFNCAY 202
             +C    G +A  LF  LYLV+LG G +K ++ SHGA+QF    P   ++ STFFN   
Sbjct: 135 ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYV 194

Query: 203 FAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGTIFTP 262
           F    G L+A+T +VW++ + G + GF VST ++ L ++  L G+ F++N  PRG+  T 
Sbjct: 195 FCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTT 254

Query: 263 IAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN--FRFLDKACIKSEEGRVGSEE 322
           I +V +AA +     C S +   H +    V+  H +    + L  +     +   G   
Sbjct: 255 IFKVLLAASIVS---CSSKTSSNHFTSR-EVQSEHEEKTPSQSLTNSLTCLNKAIEGKTH 314

Query: 323 SPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNSFQIPP 382
             W  CTV QVE VKI+L ++PIF CTI+ N  LAQL T+SV Q A MN  + N F +P 
Sbjct: 315 HIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVN-FNVPS 374

Query: 383 ASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTGLFVATFSMVSAALVE 442
           ASL   P + ++ L P Y+   +P AR++T +  GI+ LQR+G GL ++  +M  AALVE
Sbjct: 375 ASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVE 434

Query: 443 KKR----RNSSIVGSS----LSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFL 502
            KR    R + ++ S     ++ LWIA Q++  G ++LFT  GL+EFF+ ++   M+S  
Sbjct: 435 LKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLA 494

Query: 503 TAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLFYWLLAGLSLVNF 562
           T++++ S + G+YLSS++V +VN++T  +    WL    LN +RLDLFYWL+  LS+VNF
Sbjct: 495 TSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGEK-LNRNRLDLFYWLMCVLSVVNF 554

BLAST of Carg23029 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.2e-103
Identity = 226/568 (39.79%), Postives = 328/568 (57.75%), Query Frame = 0

Query: 13  KEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMH 72
           +E+    ED  VD+ G P    K G   A  F+LG +  E +A   +  NLITY+ T++H
Sbjct: 19  QEVKLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLH 78

Query: 73  FPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPR 132
                +A  VT + GT +   L+G  L+D+Y G + T+  F  +   G S LT+ A  P 
Sbjct: 79  QGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPA 138

Query: 133 LRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQ 192
           L+P  C    G  C  A   +  M F  LYL+ALG+G +KP + S GADQF      +  
Sbjct: 139 LKPAEC---IGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERV 198

Query: 193 KLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRN 252
           + ++FFN  YF+   G L++ +LLVW+Q + G  +GF + T  M L + S   GT  +R 
Sbjct: 199 RKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRF 258

Query: 253 SPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQN------GVVKLLHTDNFRFLDK 312
             P G+  T I+QV VA+F K     P ++ +L+ +Q+      G  K+ HTD+ ++LDK
Sbjct: 259 QKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDK 318

Query: 313 AC-IKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQG 372
           A  I  EE + G   + W+LCTVTQVE++KI++ + PI+A  IIF+ + AQ+ T  VQQG
Sbjct: 319 AAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQG 378

Query: 373 AAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTG 432
            AMN  +  SFQ+PPA+L +     +I  VPLY+   VPLAR+ T  + G + +QR+G G
Sbjct: 379 RAMNCKI-GSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIG 438

Query: 433 LFVATFSMVSAALVEKKR----RNSSIVGSS----LSILWIAPQFIIFGISELFTAVGLV 492
           LFV+   M +AA+VE  R     +  +V S     +S+LW  PQ+ I G +E+F  +G +
Sbjct: 439 LFVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQL 498

Query: 493 EFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRL 552
           EFFY QS + M+S  +A+   + + G YLSSL+++LV   T  +   GW+S N LN+  L
Sbjct: 499 EFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDN-LNSGHL 558

Query: 553 DLFYWLLAGLSLVNFFNYLFWANRYSQE 566
           D F+WLLAGLSLVN   Y F A RY Q+
Sbjct: 559 DYFFWLLAGLSLVNMAVYFFSAARYKQK 581

BLAST of Carg23029 vs. ExPASy TrEMBL
Match: A0A6J1F2C4 (protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita moschata OX=3662 GN=LOC111438893 PE=3 SV=1)

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 576/579 (99.48%), Postives = 578/579 (99.83%), Query Frame = 0

Query: 1   MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60
           MGDALE+TKTKSKEINSMYEDT VDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALEKTKTKSKEINSMYEDTSVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120
           NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180
           FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA
Sbjct: 121 FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180

Query: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240
           DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL
Sbjct: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240

Query: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN 300
           ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN
Sbjct: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN 300

Query: 301 FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360
           FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF
Sbjct: 301 FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360

Query: 361 SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420
           SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ
Sbjct: 361 SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420

Query: 421 RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480
           RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF
Sbjct: 421 RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480

Query: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLF 540
           YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGG+SGGGWLSHNDLNNDRLDLF
Sbjct: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGASGGGWLSHNDLNNDRLDLF 540

Query: 541 YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS 580
           YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS
Sbjct: 541 YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS 579

BLAST of Carg23029 vs. ExPASy TrEMBL
Match: A0A6J1ID44 (protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita maxima OX=3661 GN=LOC111472001 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 565/579 (97.58%), Postives = 570/579 (98.45%), Query Frame = 0

Query: 1   MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60
           MGDALE+TKTKSKE+ SM EDT VDWRGRPCTSNKHGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDALEKTKTKSKELTSMSEDTSVDWRGRPCTSNKHGGMTAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120
           NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120

Query: 121 FSLLTVQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180
           FSLLTVQAHYPRLRP ACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA
Sbjct: 121 FSLLTVQAHYPRLRPLACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGA 180

Query: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240
           DQFRRQHPTQFQKLSTFFNCAYFAFCTGEL+ALTLLVWVQTHYGMDVGFTVSTAAMLLGL
Sbjct: 181 DQFRRQHPTQFQKLSTFFNCAYFAFCTGELLALTLLVWVQTHYGMDVGFTVSTAAMLLGL 240

Query: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN 300
           ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQI PSNSEMLHRSQNGVVKLLHTD 
Sbjct: 241 ISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQIYPSNSEMLHRSQNGVVKLLHTDK 300

Query: 301 FRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360
           FRFLDKACIKSEEGRVGSEES WKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF
Sbjct: 301 FRFLDKACIKSEEGRVGSEESQWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTF 360

Query: 361 SVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420
           SVQQGAAMNT LTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ
Sbjct: 361 SVQQGAAMNTCLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQ 420

Query: 421 RVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480
           RVGTGLFVATFSMVSAALVEKKRRNSS+VGSSLSILWIAPQFIIFGISELFTAVGLVEFF
Sbjct: 421 RVGTGLFVATFSMVSAALVEKKRRNSSVVGSSLSILWIAPQFIIFGISELFTAVGLVEFF 480

Query: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLF 540
           YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGG+SGGGWLSHNDLNNDRLDLF
Sbjct: 481 YKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGASGGGWLSHNDLNNDRLDLF 540

Query: 541 YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS 580
           YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS
Sbjct: 541 YWLLAGLSLVNFFNYLFWANRYSQEHEAQDLENPTSKPS 579

BLAST of Carg23029 vs. ExPASy TrEMBL
Match: A0A6J1ID04 (protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita maxima OX=3661 GN=LOC111472692 PE=3 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 1.1e-275
Identity = 490/568 (86.27%), Postives = 528/568 (92.96%), Query Frame = 0

Query: 1   MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60
           MGD+LE+TK      + +  DT VDWRGRPC SNKHGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDSLEKTK------SILSVDTCVDWRGRPCNSNKHGGMGAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120
           NNLITYV+++MHFPLSK+ANVVTNFVGTVF LSLLGGFLSDSYLGSFRTMLIFGF+ELSG
Sbjct: 61  NNLITYVYSEMHFPLSKAANVVTNFVGTVFLLSLLGGFLSDSYLGSFRTMLIFGFIELSG 120

Query: 121 FSLLTVQAHYPRLRPPACDM---GTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIIS 180
           F++LTVQAHYP LRP AC+M   G G +C EAKGY+ALM F+ALYLVALGSGCLKPNIIS
Sbjct: 121 FTVLTVQAHYPGLRPAACEMGRSGEGDECEEAKGYKALMFFSALYLVALGSGCLKPNIIS 180

Query: 181 HGADQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAML 240
           HGADQF++Q PTQF+KLSTFFNCAYFAFCTGELIALTLLVWVQTH GMD+GF VSTAAML
Sbjct: 181 HGADQFQKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAML 240

Query: 241 LGLISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLH 300
           L LISLL+GTSFFRN+PPRG+IFTPIAQVFVAAF KRKQICPSNSEMLH S NGVVKLLH
Sbjct: 241 LALISLLSGTSFFRNNPPRGSIFTPIAQVFVAAFTKRKQICPSNSEMLHGSHNGVVKLLH 300

Query: 301 TDNFRFLDKACIKSEEGRVGS-EESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQ 360
           TDNFRFLDKACIKSEE R G+ EESPWKLCTVTQVEQVKIILS++PIFACTIIFNTILAQ
Sbjct: 301 TDNFRFLDKACIKSEETRAGTREESPWKLCTVTQVEQVKIILSVVPIFACTIIFNTILAQ 360

Query: 361 LQTFSVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGI 420
           LQTFSVQQGA+MNT+LTNSFQIPPASLQSIPY+ILIFLVPLYETAFVPLARR+T T+SGI
Sbjct: 361 LQTFSVQQGASMNTTLTNSFQIPPASLQSIPYLILIFLVPLYETAFVPLARRLTATDSGI 420

Query: 421 SPLQRVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGL 480
           +PLQRVGTGLFVATFSMVSAALVE+KRR+S    S+LSILWIAPQF+IFGISELFTAVGL
Sbjct: 421 TPLQRVGTGLFVATFSMVSAALVEQKRRHS----SNLSILWIAPQFVIFGISELFTAVGL 480

Query: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDR 540
           VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVS+VNK++ G SGGGWLSHNDLN DR
Sbjct: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSVVNKVSRGESGGGWLSHNDLNKDR 540

Query: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQ 565
           LDLFYWLLAGLSLVNFFNYLFWANRYSQ
Sbjct: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQ 558

BLAST of Carg23029 vs. ExPASy TrEMBL
Match: A0A6J1EUC1 (protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita moschata OX=3662 GN=LOC111436690 PE=3 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 2.4e-275
Identity = 490/568 (86.27%), Postives = 528/568 (92.96%), Query Frame = 0

Query: 1   MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60
           MGD+LE+TK      + +  DT VDWRGRPC SNKHGGM AAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDSLEKTK------SILSVDTCVDWRGRPCNSNKHGGMGAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120
           NNLITYV+++MHFPLSK+ANVVTNFVGTVF LSLLGGFLSDSYLGSFRTMLIFGF+ELSG
Sbjct: 61  NNLITYVYSEMHFPLSKAANVVTNFVGTVFLLSLLGGFLSDSYLGSFRTMLIFGFIELSG 120

Query: 121 FSLLTVQAHYPRLRPPACDMGT---GQQCMEAKGYEALMLFTALYLVALGSGCLKPNIIS 180
           F++LTVQAHYP LRP AC+MG+   G +C EAKGY+ALM F+ALYLVALGSGCLKPNIIS
Sbjct: 121 FTVLTVQAHYPGLRPAACEMGSSGEGDECEEAKGYKALMFFSALYLVALGSGCLKPNIIS 180

Query: 181 HGADQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAML 240
           HGADQF++Q PTQF+KLSTFFNCAYFAFCTGELIALTLLVWVQTH GMD+GF VSTAAML
Sbjct: 181 HGADQFQKQDPTQFKKLSTFFNCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAML 240

Query: 241 LGLISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLH 300
           L LISLL+G SFFRN+PPRG+IFTPIAQVFVAAF KRKQICPSNSEMLH S NGVVKLLH
Sbjct: 241 LALISLLSGASFFRNNPPRGSIFTPIAQVFVAAFTKRKQICPSNSEMLHGSHNGVVKLLH 300

Query: 301 TDNFRFLDKACIKSEEGRVGS-EESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQ 360
           TDNFRFLDKACIKSEE R G+ EESPWKLCTVTQVEQVKIILS+IPIFACTIIFNTILAQ
Sbjct: 301 TDNFRFLDKACIKSEETRGGTREESPWKLCTVTQVEQVKIILSVIPIFACTIIFNTILAQ 360

Query: 361 LQTFSVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGI 420
           LQTFSVQQGA+MNT+LTNSFQIPPASLQSIPY+ILIFLVPLYETAFVPLARR+T T+SGI
Sbjct: 361 LQTFSVQQGASMNTTLTNSFQIPPASLQSIPYLILIFLVPLYETAFVPLARRLTATDSGI 420

Query: 421 SPLQRVGTGLFVATFSMVSAALVEKKRRNSSIVGSSLSILWIAPQFIIFGISELFTAVGL 480
           +PLQRVGTGLFVATFSMVSAALVE+KRR+S    S+LSILWIAPQF+IFGISELFTAVGL
Sbjct: 421 TPLQRVGTGLFVATFSMVSAALVEQKRRHS----SNLSILWIAPQFVIFGISELFTAVGL 480

Query: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDR 540
           VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVS+VNK++GG SGGGWLSHNDLN DR
Sbjct: 481 VEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSVVNKVSGGGSGGGWLSHNDLNKDR 540

Query: 541 LDLFYWLLAGLSLVNFFNYLFWANRYSQ 565
           LDLFYWLLAGLSLVNF NYLFWANRYSQ
Sbjct: 541 LDLFYWLLAGLSLVNFINYLFWANRYSQ 558

BLAST of Carg23029 vs. ExPASy TrEMBL
Match: A0A6J1E215 (protein NRT1/ PTR FAMILY 4.4-like OS=Momordica charantia OX=3673 GN=LOC111025710 PE=3 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 3.2e-264
Identity = 475/571 (83.19%), Postives = 508/571 (88.97%), Query Frame = 0

Query: 1   MGDALERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVG 60
           MGD LE+TK+   + NS    T VDWRGR C+SN HGGMAAAAFVLGLQAFEMMAIAAVG
Sbjct: 1   MGDVLEKTKSSKLKENS----TSVDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVG 60

Query: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSG 120
           NNLITYVFTQMHFPLSKSANVVTNFVGTVF LSLLGGF+SDSYLGSFRTML+FG +ELSG
Sbjct: 61  NNLITYVFTQMHFPLSKSANVVTNFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSG 120

Query: 121 FSLLTVQAHYPRLRPPACDM-GTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHG 180
           F+LLTVQAHYP LRP  CDM  T   C+EA G EA M F ALYLVALGSGCLKPNIISHG
Sbjct: 121 FTLLTVQAHYPGLRPAPCDMAATSTPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHG 180

Query: 181 ADQFRRQHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLG 240
           ADQF ++ P Q +KLSTFFNCAYFAFCTGEL ALTLLVW+QTH GMDVGF VSTAAMLLG
Sbjct: 181 ADQFPKEDPKQLKKLSTFFNCAYFAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLG 240

Query: 241 LISLLAGTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTD 300
           LI LL+ TS +RN+PPRGTIFTPIAQVFVAAF KRKQICPSNSEMLH ++NG++KLLHTD
Sbjct: 241 LICLLSATSLYRNTPPRGTIFTPIAQVFVAAFTKRKQICPSNSEMLHGTENGLLKLLHTD 300

Query: 301 NFRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQT 360
            FRFLDKACIKSE+   G EESPWKLCTV QVEQVKIILS+IPIFACTIIFNTILAQLQT
Sbjct: 301 KFRFLDKACIKSEDDE-GREESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQT 360

Query: 361 FSVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPL 420
           FSVQQG +M++ LT  FQIPPASLQ+IPY+ILIFLVPLYETAFVPLARR+T   SGISPL
Sbjct: 361 FSVQQGTSMDSWLTPKFQIPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPL 420

Query: 421 QRVGTGLFVATFSMVSAALVEKKRRNSSIVGS-SLSILWIAPQFIIFGISELFTAVGLVE 480
           QRVGTGLFVATFSMVSAALVE KRRNSS  G+  LSILWI+PQF+IFGISELFTAVGLVE
Sbjct: 421 QRVGTGLFVATFSMVSAALVETKRRNSSAAGAPPLSILWISPQFVIFGISELFTAVGLVE 480

Query: 481 FFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLD 540
           FFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKIT GS+ GGWLSHNDLNNDRLD
Sbjct: 481 FFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLD 540

Query: 541 LFYWLLAGLSLVNFFNYLFWANRYSQEHEAQ 570
           LFYWLLAGLS VNFFNYLFWANRYSQ H  +
Sbjct: 541 LFYWLLAGLSFVNFFNYLFWANRYSQGHRGE 566

BLAST of Carg23029 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 778.1 bits (2008), Expect = 5.0e-225
Identity = 403/557 (72.35%), Postives = 465/557 (83.48%), Query Frame = 0

Query: 20  EDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSA 79
           +D  VD+RGRPC  +KHGG  AA FVLG QAFEMMAIAAVGNNLITYVF +MHFPLSKSA
Sbjct: 23  DDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSA 82

Query: 80  NVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPACD 139
           N+VTNF+GTVF LSLLGGFLSDSYLGSFRTML+FG +E+SGF LL+VQAH P LRPP C+
Sbjct: 83  NLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECN 142

Query: 140 M-GTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFF 199
           M  T   C+EA GY+A  L+TAL LVALGSGCLKPNIISHGA+QF+R+     +KLS+FF
Sbjct: 143 MKSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRK---DLRKLSSFF 202

Query: 200 NCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGT 259
           N AYFAF  G+LIALTLLVWVQTH GMDVGF VS A M  G+ISL+AGTSF+RN PP G+
Sbjct: 203 NAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGS 262

Query: 260 IFTPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVK---LLHTDNFRFLDKACIKSEEGR 319
           IFTPIAQVFVAA  KRKQICPSN  M+H+    +V+   LLH++ FRFLDKACIK+ +G+
Sbjct: 263 IFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIKT-QGK 322

Query: 320 VGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNS 379
             + ESPW+LCT+ QV QVKI+LS+IPIFACTIIFNTILAQLQTFSVQQG++MNT +T +
Sbjct: 323 --AMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKT 382

Query: 380 FQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTGLFVATFSMVS 439
           FQIPPASLQ+IPYIILIF VPLYET FVPLAR++T  +SGISPLQR+GTGLF+ATFSMV+
Sbjct: 383 FQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVA 442

Query: 440 AALVEKKRRNSSIVGS-SLSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFLTA 499
           AALVEKKRR S +  +  LSI WIAPQF+IFG+SE+FTAVGLVEFFYKQS + MQSFLTA
Sbjct: 443 AALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTA 502

Query: 500 MTYCSYSFGFYLSSLLVSLVNKITGGSSGG---GWLSHNDLNNDRLDLFYWLLAGLSLVN 559
           MTYCSYSFGFYLSS+LVS VN++T  +  G   GWL  NDLN DRLD FYWLLA LS +N
Sbjct: 503 MTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFIN 562

Query: 560 FFNYLFWANRYSQEHEA 569
           FFNYLFW+  YS +  A
Sbjct: 563 FFNYLFWSRWYSCDPSA 573

BLAST of Carg23029 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 740.0 bits (1909), Expect = 1.5e-213
Identity = 377/562 (67.08%), Postives = 457/562 (81.32%), Query Frame = 0

Query: 6   ERTKTKSKEINSMYEDTFVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLIT 65
           E+ +  ++    + E+  VDWRGRP   NKHGGM AA FVLGLQAFE+M IAAVGNNLIT
Sbjct: 12  EQEEVSNENNWELAEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLIT 71

Query: 66  YVFTQMHFPLSKSANVVTNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLT 125
           YV  +MHFPLSK+AN+VTNFVGT+F  +LLGG+LSD++LGSF T++IFGF+ELSGF LL+
Sbjct: 72  YVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLS 131

Query: 126 VQAHYPRLRPPACDMGTGQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRR 185
           VQAH P+L+PP C+    Q C EAKG++A++ F ALYLVALGSGC+KPN+I+HGADQF +
Sbjct: 132 VQAHLPQLKPPKCNPLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQ 191

Query: 186 QHPTQFQKLSTFFNCAYFAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLA 245
            HP Q ++LS++FN AYFAF  GELIALTLLVWVQTH GMD+GF VS AAM +G+ISL++
Sbjct: 192 SHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVS 251

Query: 246 GTSFFRNSPPRGTIFTPIAQVFVAAFVKRKQICPSNSEMLHRSQ---NGVV---KLLHTD 305
           GT +FRN  PR +IFTPIA V VAA +KRK   PS+  MLH      N VV    L HT 
Sbjct: 252 GTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTP 311

Query: 306 NFRFLDKACIKSEEGRVGSEESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQT 365
            FRFLDKACIK ++    ++ESPW+LCTVTQVEQVK ++SL+PIFA TI+FNTILAQLQT
Sbjct: 312 RFRFLDKACIKIQD--TNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQT 371

Query: 366 FSVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPL 425
           FSVQQG++MNT L+NSF IPPASLQ+IPYI+LIFLVPLY++  VP AR++T  NSGI PL
Sbjct: 372 FSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPL 431

Query: 426 QRVGTGLFVATFSMVSAALVEKKRRNSSIV-GSSLSILWIAPQFIIFGISELFTAVGLVE 485
            R+G GLF++TFSMVSAA++EKKRR+SS++ G  LSI WI PQF+IFGISE+FTAVGL+E
Sbjct: 432 TRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIE 491

Query: 486 FFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGS-SGGGWLSHNDLNNDRL 545
           FFYKQS +GM+SFL A+TYCSYSFGFY SS+LVS+VNKIT  S    GWL  NDLN DRL
Sbjct: 492 FFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRL 551

Query: 546 DLFYWLLAGLSLVNFFNYLFWA 560
           DLFYWLLA LSL+NF +YLFW+
Sbjct: 552 DLFYWLLAVLSLLNFLSYLFWS 571

BLAST of Carg23029 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 417.9 bits (1073), Expect = 1.3e-116
Identity = 244/580 (42.07%), Postives = 342/580 (58.97%), Query Frame = 0

Query: 23  FVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVV 82
           + DWR R     +HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH   SKSAN V
Sbjct: 13  YADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDV 72

Query: 83  TNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPACDMGT 142
           TNF+GT F L+LLGGFLSD++  +F+  LI   +E  G  +LT+QA  P L PP+CD  T
Sbjct: 73  TNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCDSPT 132

Query: 143 GQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFFNCAY 202
              C E  G +A MLF  LYLVALG G +K ++ SHGA+QF    P   ++ STFFN  Y
Sbjct: 133 ---CEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFN--Y 192

Query: 203 FAFC--TGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGTIF 262
           F FC   G L+A+T +VW++ + G + GF VST A+ + ++  L+G+ F+RN  P G+  
Sbjct: 193 FVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPL 252

Query: 263 TPIAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDNFRFLDKACIKSEEGRVGSEE 322
           T I +V +AA VK    C S S     S N V  +  + +   + K   K E    G  E
Sbjct: 253 TTILKVLLAASVK---CCSSGS-----SSNAVASMSVSPSNHCVSKG--KKEVESQGELE 312

Query: 323 SPWK------------------------------LCTVTQVEQVKIILSLIPIFACTIIF 382
            P +                               CTV QVE VKI+L ++PIFACTI+ 
Sbjct: 313 KPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIML 372

Query: 383 NTILAQLQTFSVQQGAAMNTSLTNSFQIPPASLQSIPYIILIFLVPLYETAFVPLARRIT 442
           N  LAQL TFSVQQ A+MNT +  S +IPPASL   P + ++ L P+Y+   +P AR+ T
Sbjct: 373 NCCLAQLSTFSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKAT 432

Query: 443 HTNSGISPLQRVGTGLFVATFSMVSAALVEKKR----RNSSIVGSS----LSILWIAPQF 502
            T +G++ LQR+G GL ++  +M  AALVE KR    ++S ++ S     ++ LWIA Q+
Sbjct: 433 KTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQY 492

Query: 503 IIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITGGSS 562
           +  G ++LFT  GL+E+F+ ++   M+S  T++++ S + G+YLSS++VS+VN ITG S 
Sbjct: 493 LFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSG 552

BLAST of Carg23029 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 397.5 bits (1020), Expect = 1.8e-110
Identity = 220/550 (40.00%), Postives = 326/550 (59.27%), Query Frame = 0

Query: 23  FVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVV 82
           + DWR +     +HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH  L++S++ V
Sbjct: 11  YADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEV 70

Query: 83  TNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPACDMGT 142
           T F+ T F L+LLGGFL+D++  +F   LI   +E  G  LLT+QA  P L PP C    
Sbjct: 71  TTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSA 130

Query: 143 GQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFFNCAY 202
             +C    G +A  LF  LYLV+LG G +K ++ SHGA+QF    P   ++ STFFN   
Sbjct: 131 ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYV 190

Query: 203 FAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGTIFTP 262
           F    G L+A+T +VW++ + G + GF VST ++ L ++  L G+ F++N  PRG+  T 
Sbjct: 191 FCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTT 250

Query: 263 IAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN--FRFLDKACIKSEEGRVGSEE 322
           I +V +AA +     C S +   H +    V+  H +    + L  +     +   G   
Sbjct: 251 IFKVLLAASIVS---CSSKTSSNHFTSR-EVQSEHEEKTPSQSLTNSLTCLNKAIEGKTH 310

Query: 323 SPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNSFQIPP 382
             W  CTV QVE VKI+L ++PIF CTI+ N  LAQL T+SV Q A MN  + N F +P 
Sbjct: 311 HIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVN-FNVPS 370

Query: 383 ASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTGLFVATFSMVSAALVE 442
           ASL   P + ++ L P Y+   +P AR++T +  GI+ LQR+G GL ++  +M  AALVE
Sbjct: 371 ASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVE 430

Query: 443 KKR----RNSSIVGSS----LSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFL 502
            KR    R + ++ S     ++ LWIA Q++  G ++LFT  GL+EFF+ ++   M+S  
Sbjct: 431 LKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLA 490

Query: 503 TAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLFYWLLAGLSLVNF 562
           T++++ S + G+YLSS++V +VN++T  +    WL    LN +RLDLFYWL+  LS+VNF
Sbjct: 491 TSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGEK-LNRNRLDLFYWLMCVLSVVNF 550

BLAST of Carg23029 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 397.5 bits (1020), Expect = 1.8e-110
Identity = 220/550 (40.00%), Postives = 326/550 (59.27%), Query Frame = 0

Query: 23  FVDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVV 82
           + DWR +     +HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH  L++S++ V
Sbjct: 15  YADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEV 74

Query: 83  TNFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPACDMGT 142
           T F+ T F L+LLGGFL+D++  +F   LI   +E  G  LLT+QA  P L PP C    
Sbjct: 75  TTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSA 134

Query: 143 GQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFFNCAY 202
             +C    G +A  LF  LYLV+LG G +K ++ SHGA+QF    P   ++ STFFN   
Sbjct: 135 ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYV 194

Query: 203 FAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGTIFTP 262
           F    G L+A+T +VW++ + G + GF VST ++ L ++  L G+ F++N  PRG+  T 
Sbjct: 195 FCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTT 254

Query: 263 IAQVFVAAFVKRKQICPSNSEMLHRSQNGVVKLLHTDN--FRFLDKACIKSEEGRVGSEE 322
           I +V +AA +     C S +   H +    V+  H +    + L  +     +   G   
Sbjct: 255 IFKVLLAASIVS---CSSKTSSNHFTSR-EVQSEHEEKTPSQSLTNSLTCLNKAIEGKTH 314

Query: 323 SPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNSFQIPP 382
             W  CTV QVE VKI+L ++PIF CTI+ N  LAQL T+SV Q A MN  + N F +P 
Sbjct: 315 HIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVN-FNVPS 374

Query: 383 ASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTGLFVATFSMVSAALVE 442
           ASL   P + ++ L P Y+   +P AR++T +  GI+ LQR+G GL ++  +M  AALVE
Sbjct: 375 ASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVE 434

Query: 443 KKR----RNSSIVGSS----LSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFL 502
            KR    R + ++ S     ++ LWIA Q++  G ++LFT  GL+EFF+ ++   M+S  
Sbjct: 435 LKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLA 494

Query: 503 TAMTYCSYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLFYWLLAGLSLVNF 562
           T++++ S + G+YLSS++V +VN++T  +    WL    LN +RLDLFYWL+  LS+VNF
Sbjct: 495 TSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGEK-LNRNRLDLFYWLMCVLSVVNF 554

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037184.10.0e+00100.00Protein NRT1/ PTR FAMILY 4.4, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022932490.10.0e+0099.48protein NRT1/ PTR FAMILY 4.4-like [Cucurbita moschata][more]
XP_022973463.10.0e+0097.58protein NRT1/ PTR FAMILY 4.4-like [Cucurbita maxima][more]
XP_023523828.10.0e+0097.06protein NRT1/ PTR FAMILY 4.4-like [Cucurbita pepo subsp. pepo][more]
KAG6607544.11.6e-28199.40Protein NRT1/ PTR FAMILY 4.4, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q56XQ67.0e-22472.35Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Q93VV52.1e-21267.08Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q8H1571.8e-11542.07Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE42.6e-10940.00Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
P460321.2e-10339.79Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F2C40.0e+0099.48protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita moschata OX=3662 GN=LOC111438893 ... [more]
A0A6J1ID440.0e+0097.58protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita maxima OX=3661 GN=LOC111472001 PE... [more]
A0A6J1ID041.1e-27586.27protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita maxima OX=3661 GN=LOC111472692 PE... [more]
A0A6J1EUC12.4e-27586.27protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita moschata OX=3662 GN=LOC111436690 ... [more]
A0A6J1E2153.2e-26483.19protein NRT1/ PTR FAMILY 4.4-like OS=Momordica charantia OX=3673 GN=LOC111025710... [more]
Match NameE-valueIdentityDescription
AT1G33440.15.0e-22572.35Major facilitator superfamily protein [more]
AT1G59740.11.5e-21367.08Major facilitator superfamily protein [more]
AT1G69850.11.3e-11642.07nitrate transporter 1:2 [more]
AT1G27040.21.8e-11040.00Major facilitator superfamily protein [more]
AT1G27040.11.8e-11040.00Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 107..519
e-value: 8.7E-92
score: 308.0
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 19..569
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 10..570
e-value: 5.8E-164
score: 548.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 41..563
NoneNo IPR availablePANTHERPTHR11654:SF551PROTEIN NRT1/ PTR FAMILY 4.3coord: 19..569
NoneNo IPR availableCDDcd17414MFS_NPF4coord: 39..561
e-value: 0.0
score: 625.203

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23029-RACarg23029-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006817 phosphate ion transport
biological_process GO:0050896 response to stimulus
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity