Carg23026 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23026
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionacidic mammalian chitinase-like
LocationCarg_Chr05: 11175817 .. 11176893 (+)
RNA-Seq ExpressionCarg23026
SyntenyCarg23026
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAGTTTGCGAATGTGACCGAGTTTGTGAATGCAGGGTATTGGCTTGCGAGCACCGACGACAGGCTCCCGGCTGCCAACATCGACGCTACACTCTTCACCCATCTCTTCGCTGGCTACGCTCAACTCAACTCTGATACCTACCAAGTCACCATCGATCCTTCACACCTCATCCTCATCGATCAGTTTTCTTCAACCGTCAAACTCAGCAACCCTGAGGTGAAGACTCTGTTGTCCATTGCAGCAGACGCCGCCCTCTTCGCCGCAATGGCTGCAGACCCTACAAACCGCGATGCCTTCATCAAGTCCTCCATTGCTGCAGCCAGAGGCGGTGGGTTCGATGGCCTCGACCTCCAGTGGCTATACCCTTCTTCTCAGGACCAAATGGACAACTTCAAAACCTTGATCCATTCGTGGCACCAAGCGGTGGTGGACGATGCCACCAGTTTAAGCCTCCCGCAGCTGCTCCTGGTGGCCACAGTCTCCAATCTTCCCTATCTTCAGAGCAACATTCAATACCCAGCTGATGCAATCAACCAAAAGCTGGACTGGGTGAACCTCCTCTCTTATGACTTTTACACCCCTGACTCCTCCAGGGCTGCCACAGGGCCATCCTCTGCCTTCCACAATCCCAAGGCCAATGCCCTTTCAGCTAATTTTGGGATCAGGTCGTGGGTGCAGAGCCTCCACAATCTGCCTCACAAGAAAATCGTGTTTGGAATTCCCTTCCACGGGTGGGCTTGGAAGCTTTTCAACTCTCACCAGCACACCCTGTTTGCTAAAGCGGATGGAGCCGCCACCGGAGAATACATTGGTCCACACGGCCAAATCTTCTACTCCGATGTGCAGAAATTTATAGAGGACAATCAGGCTGGTGTTGAGGACAAGGACAAGAGCTATCTTGTTGCCTACGCTTATTACGAGACAACCTGGATTACCTACGAGAGTGAATACACCATTGCCGACAAGATCGCACTCGCAAAGACCGACAAGGTTCTTGGTTACTTTGCTTTCAACATTGCAGCCGATGACGCTTCTAACACTCTTGCTAATGCTGCCAAATCCAATTGGTGA

mRNA sequence

ATGGCTGAGTTTGCGAATGTGACCGAGTTTGTGAATGCAGGGTATTGGCTTGCGAGCACCGACGACAGGCTCCCGGCTGCCAACATCGACGCTACACTCTTCACCCATCTCTTCGCTGGCTACGCTCAACTCAACTCTGATACCTACCAAGTCACCATCGATCCTTCACACCTCATCCTCATCGATCAGTTTTCTTCAACCGTCAAACTCAGCAACCCTGAGGTGAAGACTCTGTTGTCCATTGCAGCAGACGCCGCCCTCTTCGCCGCAATGGCTGCAGACCCTACAAACCGCGATGCCTTCATCAAGTCCTCCATTGCTGCAGCCAGAGGCGGTGGGTTCGATGGCCTCGACCTCCAGTGGCTATACCCTTCTTCTCAGGACCAAATGGACAACTTCAAAACCTTGATCCATTCGTGGCACCAAGCGGTGGTGGACGATGCCACCAGTTTAAGCCTCCCGCAGCTGCTCCTGGTGGCCACAGTCTCCAATCTTCCCTATCTTCAGAGCAACATTCAATACCCAGCTGATGCAATCAACCAAAAGCTGGACTGGGTGAACCTCCTCTCTTATGACTTTTACACCCCTGACTCCTCCAGGGCTGCCACAGGGCCATCCTCTGCCTTCCACAATCCCAAGGCCAATGCCCTTTCAGCTAATTTTGGGATCAGGTCGTGGGTGCAGAGCCTCCACAATCTGCCTCACAAGAAAATCGTGTTTGGAATTCCCTTCCACGGGTGGGCTTGGAAGCTTTTCAACTCTCACCAGCACACCCTGTTTGCTAAAGCGGATGGAGCCGCCACCGGAGAATACATTGGTCCACACGGCCAAATCTTCTACTCCGATGTGCAGAAATTTATAGAGGACAATCAGGCTGGTGTTGAGGACAAGGACAAGAGCTATCTTGTTGCCTACGCTTATTACGAGACAACCTGGATTACCTACGAGAGTGAATACACCATTGCCGACAAGATCGCACTCGCAAAGACCGACAAGGTTCTTGGTTACTTTGCTTTCAACATTGCAGCCGATGACGCTTCTAACACTCTTGCTAATGCTGCCAAATCCAATTGGTGA

Coding sequence (CDS)

ATGGCTGAGTTTGCGAATGTGACCGAGTTTGTGAATGCAGGGTATTGGCTTGCGAGCACCGACGACAGGCTCCCGGCTGCCAACATCGACGCTACACTCTTCACCCATCTCTTCGCTGGCTACGCTCAACTCAACTCTGATACCTACCAAGTCACCATCGATCCTTCACACCTCATCCTCATCGATCAGTTTTCTTCAACCGTCAAACTCAGCAACCCTGAGGTGAAGACTCTGTTGTCCATTGCAGCAGACGCCGCCCTCTTCGCCGCAATGGCTGCAGACCCTACAAACCGCGATGCCTTCATCAAGTCCTCCATTGCTGCAGCCAGAGGCGGTGGGTTCGATGGCCTCGACCTCCAGTGGCTATACCCTTCTTCTCAGGACCAAATGGACAACTTCAAAACCTTGATCCATTCGTGGCACCAAGCGGTGGTGGACGATGCCACCAGTTTAAGCCTCCCGCAGCTGCTCCTGGTGGCCACAGTCTCCAATCTTCCCTATCTTCAGAGCAACATTCAATACCCAGCTGATGCAATCAACCAAAAGCTGGACTGGGTGAACCTCCTCTCTTATGACTTTTACACCCCTGACTCCTCCAGGGCTGCCACAGGGCCATCCTCTGCCTTCCACAATCCCAAGGCCAATGCCCTTTCAGCTAATTTTGGGATCAGGTCGTGGGTGCAGAGCCTCCACAATCTGCCTCACAAGAAAATCGTGTTTGGAATTCCCTTCCACGGGTGGGCTTGGAAGCTTTTCAACTCTCACCAGCACACCCTGTTTGCTAAAGCGGATGGAGCCGCCACCGGAGAATACATTGGTCCACACGGCCAAATCTTCTACTCCGATGTGCAGAAATTTATAGAGGACAATCAGGCTGGTGTTGAGGACAAGGACAAGAGCTATCTTGTTGCCTACGCTTATTACGAGACAACCTGGATTACCTACGAGAGTGAATACACCATTGCCGACAAGATCGCACTCGCAAAGACCGACAAGGTTCTTGGTTACTTTGCTTTCAACATTGCAGCCGATGACGCTTCTAACACTCTTGCTAATGCTGCCAAATCCAATTGGTGA

Protein sequence

MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW
Homology
BLAST of Carg23026 vs. NCBI nr
Match: KAG7030350.1 (Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 722.6 bits (1864), Expect = 1.6e-204
Identity = 358/358 (100.00%), Postives = 358/358 (100.00%), Query Frame = 0

Query: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60
           MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60

Query: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120
           IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ
Sbjct: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180
           WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN
Sbjct: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180

Query: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVF 240
           QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVF
Sbjct: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVF 240

Query: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300
           GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS
Sbjct: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300

Query: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
           YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW
Sbjct: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Carg23026 vs. NCBI nr
Match: KAG6599434.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 719.5 bits (1856), Expect = 1.4e-203
Identity = 356/358 (99.44%), Postives = 357/358 (99.72%), Query Frame = 0

Query: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60
           MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60

Query: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120
           IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ
Sbjct: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180
           WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYL+SNIQYPADAIN
Sbjct: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLKSNIQYPADAIN 180

Query: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVF 240
           QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSW QSLHNLPHKKIVF
Sbjct: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVF 240

Query: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300
           GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS
Sbjct: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300

Query: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
           YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW
Sbjct: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Carg23026 vs. NCBI nr
Match: KAG6599362.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1 Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 718.0 bits (1852), Expect = 4.0e-203
Identity = 356/358 (99.44%), Postives = 356/358 (99.44%), Query Frame = 0

Query: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60
           MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPS LIL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLIL 60

Query: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120
           IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ
Sbjct: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180
           WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN
Sbjct: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180

Query: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVF 240
           QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSW QSLHNLPHKKIVF
Sbjct: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVF 240

Query: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300
           GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS
Sbjct: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300

Query: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
           YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW
Sbjct: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Carg23026 vs. NCBI nr
Match: XP_022946077.1 (acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mammalian chitinase-like [Cucurbita moschata])

HSP 1 Score: 713.8 bits (1841), Expect = 7.6e-202
Identity = 353/358 (98.60%), Postives = 355/358 (99.16%), Query Frame = 0

Query: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60
           MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNS TYQVT+DPS LIL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLIL 60

Query: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120
           IDQFSSTVKLSNPEVKTLLSIAADAA+FAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ
Sbjct: 61  IDQFSSTVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180
           WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN
Sbjct: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180

Query: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVF 240
           QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSW QSLHNLPHKKIVF
Sbjct: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVF 240

Query: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300
           GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS
Sbjct: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300

Query: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
           YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW
Sbjct: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Carg23026 vs. NCBI nr
Match: XP_023521369.1 (acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 692.2 bits (1785), Expect = 2.4e-195
Identity = 339/356 (95.22%), Postives = 350/356 (98.31%), Query Frame = 0

Query: 3   EFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILID 62
           +FANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPS LIL++
Sbjct: 9   KFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLN 68

Query: 63  QFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWL 122
           QFS TV+LSNP+VKTLLSIAADAA+FAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWL
Sbjct: 69  QFSPTVRLSNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWL 128

Query: 123 YPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQK 182
           YPSSQ++M NFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQK
Sbjct: 129 YPSSQEEMGNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQK 188

Query: 183 LDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFGI 242
           LDWVNLLSYDFYTPDS+RAATGPSSAFHNPKANALSANFGIRSW QSLHNLPHKKIVFGI
Sbjct: 189 LDWVNLLSYDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGI 248

Query: 243 PFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 302
           PFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL
Sbjct: 249 PFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 308

Query: 303 VAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
           VAY Y+ETTWITYES+YTIADKIALAK DKVLGYFAFNIAADDASNTLANAAKSNW
Sbjct: 309 VAYTYFETTWITYESDYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 364

BLAST of Carg23026 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.8e-71
Identity = 156/353 (44.19%), Postives = 211/353 (59.77%), Query Frame = 0

Query: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSSTVKL 70
           V A YW  +++   P  +ID++LFTHLF  +A LNS T QVT+  ++      F+ TV+ 
Sbjct: 28  VKASYWFPASE--FPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 71  SNPEVKTLLSIA---ADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQ 130
            NP VKTLLSI    AD   +A+MA++PT+R +FI SSI  AR  GF GLDL W YPSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 131 DQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATV--SNLPYLQSNIQYPADAINQKLDW 190
            +M NF TL+  W  AVV +A+S   P+LLL A V  SN  Y   ++ YP  A+   LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 191 VNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFGIPFH 250
           VNL++YDFY P  SR  TGP +A  +P     S + G RSW+Q+   LP KK V G P++
Sbjct: 208 VNLMAYDFYGPGWSR-VTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPYY 267

Query: 251 GWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAY 310
           G+AW+L N++ H+ +A   GAA    I P G I Y  ++KFI DN A     + + +  Y
Sbjct: 268 GYAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGA-TTVYNSTVVGDY 327

Query: 311 AYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
            Y  T WI Y+   +I  K+  AK   +LGYF++++ ADD S  L+ AA   W
Sbjct: 328 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDNSG-LSRAASQAW 366

BLAST of Carg23026 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.5e-46
Identity = 115/350 (32.86%), Postives = 186/350 (53.14%), Query Frame = 0

Query: 15  YWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSSTVKLSNPE 74
           YWL   +   P   +D +LFTH+F  +   N+ TY++ I  S ++ ++ F+ T K  +P 
Sbjct: 39  YWL--ENPFFPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKTKSPP 98

Query: 75  VKTLLSI---AADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQMD 134
             TL SI    ++++L A +A+DP    AFI S+I  AR  GFDG+DL W +P +  +M+
Sbjct: 99  AATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNTKEMN 158

Query: 135 NFKTLIHSWHQAVVDDATSLSLPQLLLVATV---SNLPYLQSNIQYPADAINQKLDWVNL 194
           +   ++  W +A+ D+  +   P LLL A V    N         YP ++IN+ LDWVN+
Sbjct: 159 DLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLDWVNV 218

Query: 195 LSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFGIPFHGWA 254
           +SY+   P S++  TG  S   +PK+N +S   G+ SW+ S   +  +K+V G+P +G +
Sbjct: 219 MSYELRGPRSNK--TGAPSGTFDPKSN-VSVVSGLLSWIHS--GVVPEKLVMGMPLYGKS 278

Query: 255 WKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYY 314
           WKL + + H + A + G+      G +G + Y  V  F     A VE  D      Y+Y 
Sbjct: 279 WKLRDPNVHGIGAPSVGSGP----GVNGLMAYFQVLDFNRQKSAKVE-YDVDTASVYSYS 338

Query: 315 ETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
            +TWI Y++ +T++ K+  A+  K+ GYF +    D     +A  A   W
Sbjct: 339 GSTWIGYDNPFTVSIKVGFAQALKLRGYFFWVAGLDTLDWKIATQASKAW 376

BLAST of Carg23026 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 178.3 bits (451), Expect = 1.5e-43
Identity = 122/361 (33.80%), Postives = 185/361 (51.25%), Query Frame = 0

Query: 8   TEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILI----DQ 67
           T  V   YW+   +   P ++ID +LFTH+F  +   N  TY++  +      +      
Sbjct: 34  TTRVKGIYWI--ENPLFPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSLTT 93

Query: 68  FSSTVKLSNPEVKTLLSI---AADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 127
           F++T K   P + TLLSI    +++ LFA +A+DPT R  FI S+I  AR  GFDG+D  
Sbjct: 94  FTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFD 153

Query: 128 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATV---SNLPYLQSNIQYPAD 187
           W +P++  +M++   L+  W +A+ D+  S S P LLL A V    N         YP D
Sbjct: 154 WEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVD 213

Query: 188 AINQKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKK 247
           +IN+ LDWVN++SYD     S+   TG  S   + K+N +S   G+ SW++    +  +K
Sbjct: 214 SINKNLDWVNVMSYDLRGSGSN--VTGAPSGMFDSKSN-VSVVSGLFSWIRG--GVAPEK 273

Query: 248 IVFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDK 307
           IV G+P +G +WKL + + H + A   G       G  G + Y  V  F +   A V   
Sbjct: 274 IVMGMPLYGKSWKLQDPNVHGIGAPNVGPGP----GVDGGMAYFQVVDFNKQMGAKVV-Y 333

Query: 308 DKSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSN 359
           DK     Y+Y  +TWI Y+  +T++ K+  A+  K+ GYF F  A  D S+   + A   
Sbjct: 334 DKETGSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYF-FWAAGYDTSDWKVSTASKA 381

BLAST of Carg23026 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 162.9 bits (411), Expect = 6.6e-39
Identity = 114/359 (31.75%), Postives = 186/359 (51.81%), Query Frame = 0

Query: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSSTVKL 70
           V A YW A +D     ++ID   FTH++  + Q   +++ + I  S+     ++     +
Sbjct: 55  VRAAYWPAWSD--FSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYDGIHNI 114

Query: 71  SNPEVKTLLSIAA---DAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQ 130
             P V TLLSI     +A LF+ MA+   NR +FI S+I  AR   F+GLDL W +P  +
Sbjct: 115 -RPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDE 174

Query: 131 DQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNI--------QYPADAI 190
             M N   L+  W++A+V +A +    +LLL + V    Y  S I         YP  AI
Sbjct: 175 KDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAV----YFNSTISLIGNGPRSYPVRAI 234

Query: 191 NQKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIV 250
            + LDW + + +D+    ++   TG ++A ++P +N +S  +GI SW+ S   +P +K+V
Sbjct: 235 RKYLDWASPMCFDYNGAWANE--TGFNAALYDPNSN-ISTKYGIGSWIGS--GVPAEKLV 294

Query: 251 FGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDK 310
            G+P +G AW+L + + H + AKA G A    +   G + Y ++  F +D  A V   D+
Sbjct: 295 MGLPLYGRAWELKDPNDHGVGAKAVGPA----VDTDGSMDYDEILVFNKDTGAKVV-YDE 354

Query: 311 SYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
             +  Y+Y  TTWI Y+   +I  K+  A++  + GYF + I   D   T++  A + W
Sbjct: 355 VAVSFYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAI-GKDKDWTISKQASNAW 395

BLAST of Carg23026 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 9.5e-38
Identity = 115/361 (31.86%), Postives = 190/361 (52.63%), Query Frame = 0

Query: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSSTVKL 70
           V + YW A  DD  P+  ID   FTH+   + Q    ++++ I  S +     F   ++ 
Sbjct: 40  VRSAYWPAG-DDFSPSL-IDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 71  SNPEVKTLLSI---AADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQ 130
            +P VKTLLSI    +++ LF+ +A+   NR+ FI S+I  AR   FDG+DL W +P +Q
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 131 DQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQ---------YPADA 190
             M N   L   W+ A+  +A     P+LLL + V    Y  S ++         YP  A
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAV----YYNSTVRLIGKHGPRSYPTQA 219

Query: 191 INQKLDWVNLLSYDFY-TPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKK 250
           IN+ LDW + + +D++ T D++   T  ++A ++ K+  +S NFG+ SW++S   +  +K
Sbjct: 220 INKYLDWASPMCFDYHGTWDNN---TDFNAALYDSKSE-ISTNFGLHSWIKS--GVRPEK 279

Query: 251 IVFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDK 310
           +V G+  +G AW+L + + + + A+A G AT       G + Y+++ KF + + A V   
Sbjct: 280 LVMGLALYGRAWELKDPNVNGVGAEAVGPAT----DTDGSMNYNEILKFNKQSGANVV-Y 339

Query: 311 DKSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSN 359
           DK  +  Y+Y  TTWI Y+   +I  K+  AK+  + GYF F     D   +++  A + 
Sbjct: 340 DKVAISFYSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYF-FWALGKDKDWSISKQASNA 382

BLAST of Carg23026 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 3.7e-202
Identity = 353/358 (98.60%), Postives = 355/358 (99.16%), Query Frame = 0

Query: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60
           MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNS TYQVT+DPS LIL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLIL 60

Query: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120
           IDQFSSTVKLSNPEVKTLLSIAADAA+FAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ
Sbjct: 61  IDQFSSTVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180
           WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN
Sbjct: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180

Query: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVF 240
           QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSW QSLHNLPHKKIVF
Sbjct: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVF 240

Query: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300
           GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS
Sbjct: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300

Query: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
           YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW
Sbjct: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Carg23026 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.4e-193
Identity = 338/358 (94.41%), Postives = 346/358 (96.65%), Query Frame = 0

Query: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60
           MAEFANVTEFVNAGYWLASTD  LPAANIDATLFTHLFAGYAQLNSDTYQVT+DPS LIL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLIL 60

Query: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120
           IDQFSSTVKLSNP+VKTLLSIA DAA+FAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ
Sbjct: 61  IDQFSSTVKLSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180
           WLYPSSQDQM NFKTLIHSWHQAVVDDATS+SLPQLLLVATVSNLPYLQ+NIQYPADAI 
Sbjct: 121 WLYPSSQDQMANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAIT 180

Query: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVF 240
           QKLDWVNLLSYDFYTPDS RAATGPSSAFHNPKANALSANFGIRSW QSLHNL HKKIVF
Sbjct: 181 QKLDWVNLLSYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVF 240

Query: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300
           GIPFHGWAWKL NSHQH LFAKADGAA+GEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS
Sbjct: 241 GIPFHGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300

Query: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
           YLVAYAYYETTWIT+ESEYT+ADKIALAK DKVLGYFAFNIAADDASNTLANAAKSNW
Sbjct: 301 YLVAYAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Carg23026 vs. ExPASy TrEMBL
Match: A0A6J1KFT3 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.2e-189
Identity = 333/358 (93.02%), Postives = 341/358 (95.25%), Query Frame = 0

Query: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60
           MAEFANVTEFVNAGYWLASTD  LPAANIDATLFTHLFAGYAQLNSDTYQVT+DPS LIL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLIL 60

Query: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120
           IDQFSSTVKLSNP VKTLLSIA DAA+FAAMA DPTNRDAFIKSSIAAARGGGFDGLDLQ
Sbjct: 61  IDQFSSTVKLSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180
           WLYPSSQDQM NFKTLI SWHQAVVDDATS+SLPQLLLVATVSNLPYLQ+NIQYPADAI 
Sbjct: 121 WLYPSSQDQMANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAIT 180

Query: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVF 240
           Q LDWVNLLSYDFYTPDS R ATGPSSAFHNPKANALSANFGIRSW QSL+NL HKKIVF
Sbjct: 181 QNLDWVNLLSYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVF 240

Query: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300
           GIPFHGWAWKL NSHQH LFAKADGAA+GEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS
Sbjct: 241 GIPFHGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300

Query: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
           YLVAYAYYETTWIT+ESEYT+ADKIALAK DKVLGYFAFNIAADDASNTLANAAKSNW
Sbjct: 301 YLVAYAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Carg23026 vs. ExPASy TrEMBL
Match: A0A6J1G4H5 (chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 2.6e-187
Identity = 327/358 (91.34%), Postives = 342/358 (95.53%), Query Frame = 0

Query: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60
           MA+FANVT+FVNAGYWLAST+DRL AANIDATLFTHLF GYA L+  T+QVT+DPS LIL
Sbjct: 1   MAKFANVTKFVNAGYWLASTNDRLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLIL 60

Query: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120
           I+QFSSTVKLSNP+VKTLLSIAADAA+FAAMAA PTNRD FIKSSIAAARGGGFDGLDLQ
Sbjct: 61  INQFSSTVKLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQ 120

Query: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAIN 180
           WLYPSSQ +M NFKTLIHSWHQAVVDDAT +SLPQLLLVATVSNLPYLQ N++YPADAIN
Sbjct: 121 WLYPSSQYEMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAIN 180

Query: 181 QKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVF 240
           QKLDWVNLLSYDFYTPDSS+AATGPSSAFHNPKANALSANFGIRSW QSLHNLPHKKIVF
Sbjct: 181 QKLDWVNLLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVF 240

Query: 241 GIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300
           GIPFHGWAWKLFNSHQH  FAKADGAA+GEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS
Sbjct: 241 GIPFHGWAWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKS 300

Query: 301 YLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
           YLVAYAYYETTWITYESEYTIADKIALAK DKVLGYFAFNIAADDASNTLANAAKSNW
Sbjct: 301 YLVAYAYYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Carg23026 vs. ExPASy TrEMBL
Match: A0A6J1G4J9 (acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 3.8e-183
Identity = 320/352 (90.91%), Postives = 333/352 (94.60%), Query Frame = 0

Query: 7   VTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSS 66
           + EFVNAGYWLA TDD L AANIDATLFTHLF GYA L+  T+QVT+DPS LILI+QFSS
Sbjct: 1   MAEFVNAGYWLAGTDDTLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQFSS 60

Query: 67  TVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126
           TVKLSNP+VKTLLSIAADAA+FAAMAA PTNRD FIKSSIAAARGGGFDGLDLQWLYPSS
Sbjct: 61  TVKLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYPSS 120

Query: 127 QDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWV 186
           Q +M NFKTLIHSWHQAVVDDAT +SLPQLLLVATVSNLPYLQ N++YPADAINQKLDWV
Sbjct: 121 QYEMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAINQKLDWV 180

Query: 187 NLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFGIPFHG 246
           NLLSYDFYTPDSS+AATGPSSAFHNPKANALSANFGIRSW QSLHNLPHKKIVFGIPFHG
Sbjct: 181 NLLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHG 240

Query: 247 WAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 306
           WAWKLFNSHQH  FAKADGAA+GEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA
Sbjct: 241 WAWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 300

Query: 307 YYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
           YYETTWITYESEYTIADKIALAK DKVLGYFAFNIAADDASNTLANAAKSNW
Sbjct: 301 YYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 352

BLAST of Carg23026 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 270.0 bits (689), Expect = 2.7e-72
Identity = 156/353 (44.19%), Postives = 211/353 (59.77%), Query Frame = 0

Query: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSSTVKL 70
           V A YW  +++   P  +ID++LFTHLF  +A LNS T QVT+  ++      F+ TV+ 
Sbjct: 28  VKASYWFPASE--FPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 71  SNPEVKTLLSIA---ADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQ 130
            NP VKTLLSI    AD   +A+MA++PT+R +FI SSI  AR  GF GLDL W YPSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 131 DQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATV--SNLPYLQSNIQYPADAINQKLDW 190
            +M NF TL+  W  AVV +A+S   P+LLL A V  SN  Y   ++ YP  A+   LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 191 VNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFGIPFH 250
           VNL++YDFY P  SR  TGP +A  +P     S + G RSW+Q+   LP KK V G P++
Sbjct: 208 VNLMAYDFYGPGWSR-VTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPYY 267

Query: 251 GWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAY 310
           G+AW+L N++ H+ +A   GAA    I P G I Y  ++KFI DN A     + + +  Y
Sbjct: 268 GYAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGA-TTVYNSTVVGDY 327

Query: 311 AYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 359
            Y  T WI Y+   +I  K+  AK   +LGYF++++ ADD S  L+ AA   W
Sbjct: 328 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDNSG-LSRAASQAW 366

BLAST of Carg23026 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 248.8 bits (634), Expect = 6.5e-66
Identity = 142/350 (40.57%), Postives = 200/350 (57.14%), Query Frame = 0

Query: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60
           +  F++    V A YW A ++   P A ID++LFTHLF  +A +N+ TYQV +   +   
Sbjct: 16  LLRFSSAQTVVKATYWFAESES--PLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPK 75

Query: 61  IDQFSSTVKLSNPEVKTLLSIAADAA---LFAAMAADPTNRDAFIKSSIAAARGGGFDGL 120
              F+ TV+  NP VKTLLSI  D      FA+MA++PT+R  FI SSI  AR  GF GL
Sbjct: 76  FSTFTQTVRRRNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGL 135

Query: 121 DLQWLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPAD 180
           DL W YPS   +MDNF  L+  W  AV  +A S   P+LLL A V    Y   ++ +P +
Sbjct: 136 DLNWKYPSITTEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF-YSYSYYSVLHPVN 195

Query: 181 AINQKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKK 240
           A+   LDWVNL++YDFY   SSR    P +  ++P     S + G+R+W Q+   LP KK
Sbjct: 196 AVADSLDWVNLVAYDFYESGSSRVTCSP-APLYDPITTGPSGDAGVRAWTQA--GLPAKK 255

Query: 241 IVFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDK 300
            V G P +G+AW L ++  H  +A + G A    I P G I Y  +++FI DN+A +   
Sbjct: 256 AVLGFPLYGYAWCLTDAKNHNYYANSSGPA----ISPDGSIGYDQIRRFIVDNKATMV-Y 315

Query: 301 DKSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDAS 348
           + + +  Y Y + TWI Y+   +I  K+  AK   +LGYF+++I ADD S
Sbjct: 316 NSNLVQNYCYAKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADDNS 354

BLAST of Carg23026 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 235.7 bits (600), Expect = 5.7e-62
Identity = 139/346 (40.17%), Postives = 199/346 (57.51%), Query Frame = 0

Query: 8   TEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSST 67
           ++ V A YW  +TD   PA +ID++LFTHLF  +A L +++Y++TI   +      F+ T
Sbjct: 4   SDVVKASYWFPATD--FPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTET 63

Query: 68  VKLSNPEVKTLLSIA---ADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 127
           V+  NP VKTLLSI    AD   FA+MA++P +R +FI+S+I  AR  GF GLDL W YP
Sbjct: 64  VQQRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYP 123

Query: 128 SSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQ---YPADAINQ 187
            ++++M +F  L+  W  AV  ++ S     L+L A V    Y  SN Q   YP  AI+ 
Sbjct: 124 RNEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAV----YYSSNYQGVPYPVLAISN 183

Query: 188 KLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFG 247
            LDW+NL++YDFY P  S   TGP ++ + P  +  S + G+R W ++   LP KK V G
Sbjct: 184 SLDWINLMAYDFYGPGWS-TVTGPPASLYLP-TDGRSGDSGVRDWTEA--GLPAKKAVLG 243

Query: 248 IPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSY 307
            P++GWAW L +   +      D   TG  I   G+I Y  +Q +I DN A  +  D   
Sbjct: 244 FPYYGWAWTLADPDVNGY----DANTTGPAISDDGEISYRQLQTWIVDNGA-TKVHDDMM 303

Query: 308 LVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDAS 348
           +  Y Y  TTWI Y+SE +I  K+  AK   +LGYF++++  DD S
Sbjct: 304 VGDYCYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDNS 334

BLAST of Carg23026 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 231.5 bits (589), Expect = 1.1e-60
Identity = 142/368 (38.59%), Postives = 199/368 (54.08%), Query Frame = 0

Query: 3   EFANVTEFVNAGYWLASTDDR--------LPAANIDATLFTHLFAGYAQLNSDTYQVTID 62
           +F      V A YW      +         P++ ID+TLFTHLF  +A ++S T++VTI 
Sbjct: 6   QFLRTNSIVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTIS 65

Query: 63  PSHLILIDQFSSTVKLSNPEVKTLLSIA---ADAALFAAMAADPTNRDAFIKSSIAAARG 122
            ++      F+ TVK  N +V+TLLSI    AD A+ A+MA++  NR AFI SSI  AR 
Sbjct: 66  AANSYQFSSFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARK 125

Query: 123 GGFDGLDLQWLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSN 182
             F GLDL W YPS+  +M NF  L+  W  AVV+++   +   LLL A V   P     
Sbjct: 126 KDFYGLDLAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQ-YDG 185

Query: 183 IQYPADAINQKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANA-LSANFGIRSWVQSL 242
           ++YP  AI   LD+VN+++YDFY P  S     P++ FH+P   A  S N G+R W+   
Sbjct: 186 VEYPVKAIADNLDFVNIMAYDFYGPGWSPVTGPPAALFHDPSNPAGRSGNSGLRKWLDEA 245

Query: 243 HNLPHKKIVFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDN 302
             LP KK V G P+ GWAW L ++  +   A  DGAA    I P G I Y+ ++ +I DN
Sbjct: 246 -KLPPKKAVLGFPYCGWAWTLEDAENNGYDAATDGAA----ISPDGSITYAKIRNYIVDN 305

Query: 303 QAGVEDKDKSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTL 359
            A     D + +  Y Y   TWI Y+   +I  K+  AK   +LGYF++++ A D +  L
Sbjct: 306 GAAT-FHDPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGA-DYNCGL 365

BLAST of Carg23026 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 230.7 bits (587), Expect = 1.8e-60
Identity = 137/346 (39.60%), Postives = 194/346 (56.07%), Query Frame = 0

Query: 3   EFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILID 62
           +F      V A YW+   ++  PA NID+T FTHLF  +A ++S T++VTI  ++   + 
Sbjct: 6   QFLRTNSIVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVS 65

Query: 63  QFSSTVKLSNPEVKTLLSIA---ADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDL 122
            F+ TVK  N +V+TLLSI    AD A+ A+MA++  NR AFI SSI  AR   F GLDL
Sbjct: 66  SFTHTVKDKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDL 125

Query: 123 QWLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAI 182
            W YPS+  +M NF  L+  W  AVV+++   +   LLL A V   P      +YP  AI
Sbjct: 126 AWEYPSNDVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPDYYGE-EYPVQAI 185

Query: 183 NQKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANA-LSANFGIRSWVQSLHNLPHKKI 242
              LD+VN+++YDFY P  S   TGP +A  +P   A  S + G+  W+++   LP KK 
Sbjct: 186 ADNLDFVNIMAYDFYGPGWS-PVTGPPAALFDPSNPAGRSGDSGLSKWLEA--KLPAKKA 245

Query: 243 VFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKD 302
           V G  + GWAW L ++  +   A  DGAA    I   G I Y+ ++ +I DN A     D
Sbjct: 246 VLGFSYCGWAWTLEDAENNGYDAATDGAA----ISSDGSITYAKIRNYIIDNGAAT-FHD 305

Query: 303 KSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAAD 345
            + +  Y Y  TTWI Y+   +I  K+  AK   +LGYF++++ AD
Sbjct: 306 PAVIGFYCYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030350.11.6e-204100.00Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6599434.11.4e-20399.44Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6599362.14.0e-20399.44Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1... [more]
XP_022946077.17.6e-20298.60acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mamm... [more]
XP_023521369.12.4e-19595.22acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O818623.8e-7144.19Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1[more]
A0A072UR652.5e-4632.86Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1[more]
U5N4E31.5e-4333.80Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2[more]
A0A1B1J8Z26.6e-3931.75Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2[more]
A0A072VEP09.5e-3831.86Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G2P43.7e-20298.60acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE... [more]
A0A6J1KB821.4e-19394.41chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1[more]
A0A6J1KFT31.2e-18993.02chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1[more]
A0A6J1G4H52.6e-18791.34chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 ... [more]
A0A6J1G4J93.8e-18390.91acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G19810.12.7e-7244.19Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19820.16.5e-6640.57Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19800.15.7e-6240.17Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19760.11.1e-6038.59Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19750.11.8e-6039.60Glycosyl hydrolase family protein with chitinase insertion domain [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011583Chitinase IISMARTSM006362g34coord: 10..344
e-value: 1.9E-53
score: 193.6
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 27..345
e-value: 4.2E-76
score: 258.4
NoneNo IPR availablePANTHERPTHR11177CHITINASEcoord: 5..351
NoneNo IPR availablePANTHERPTHR11177:SF351CHITINASE-LIKE PROTEIN-RELATEDcoord: 5..351
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 13..344
e-value: 1.1E-49
score: 169.8
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 10..358
score: 46.405052
IPR029070Chitinase insertion domain superfamilyGENE3D3.10.50.10coord: 244..315
e-value: 4.2E-76
score: 258.4
IPR029070Chitinase insertion domain superfamilySUPERFAMILY54556Chitinase insertion domaincoord: 246..315
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 12..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23026-RACarg23026-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0008061 chitin binding
molecular_function GO:0016787 hydrolase activity