Carg23020 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23020
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type domain-containing protein
Location	Carg_Chr05: 11133025 .. 11133802 (-)
RNA-Seq Expression	Carg23020
Synteny	Carg23020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGGAATGTTGCCGGGAGTTGAATGCGCCAGAAGGCGGCGATTCCACCAGAGCATAACCTTATCGGACACGCCATCCACGGCGGCGAAATTAGGATCCACAAGAAGGCCGTCGTTCTGTTTGTACACGAGCAACCGTGATTTTCACATCACTTCATCATCGTCTTGTTCACAGGTATAAATGAATGAAATCTAAGAATTTTTCTGTTTGTTTCCCGGGAAAATTCTGATGAATGTTGAACGTAAAATTAAGCAACGGAGCTCATCCCATCAAGCTTACCAGAACGAGAAGCTTGGTGAAATTGCAAGAGAAGCTAAGGAACGGTTGGATGAGAGGTTAAGAACTCATAGAAAACCTGAAAATCCAAGGTAATTTCTTCTTTTATCGTACGAGGATCAAATGATCGTTCGAAATCTTAAAACCATAAAAACAAGTTGAAAGGAACTAAATTTGTAATTTAACCTAAAATCAACGTTGAAATCGAAAATAATCATACAGGAAAAAGAGTGGAGAAGGGTTGAAGAACTCATTGGAAAAGAAAGAAAAAGCGAAGGGGAAAGAAGAATCAATAACGAAGAAGAGGTTCACTTGGGGGAAGCTGATGAAATGGAAAGCCTCGGAGCAGGAAGAGTGCGCCGTTTGTTTGGAAGGGTTCAGAGCGGGTGAGCCGTTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCCGCGTGCTTGGTGCCTTGGCTCCAAACCAATGCCCATTGCCCATGCTGTAGGTTCCCGTTTCCCTCTTAA

mRNA sequence

ATGGCCGGAATGTTGCCGGGAGTTGAATGCGCCAGAAGGCGGCGATTCCACCAGAGCATAACCTTATCGGACACGCCATCCACGGCGGCGAAATTAGGATCCACAAGAAGGCCGTCGTTCTGTTTGTACACGAGCAACCGTGATTTTCACATCACTTCATCATCGTCTTGTTCACAGCAACGGAGCTCATCCCATCAAGCTTACCAGAACGAGAAGCTTGGTGAAATTGCAAGAGAAGCTAAGGAACGGTTGGATGAGAGGTTAAGAACTCATAGAAAACCTGAAAATCCAAGGAAAAAGAGTGGAGAAGGGTTGAAGAACTCATTGGAAAAGAAAGAAAAAGCGAAGGGGAAAGAAGAATCAATAACGAAGAAGAGGTTCACTTGGGGGAAGCTGATGAAATGGAAAGCCTCGGAGCAGGAAGAGTGCGCCGTTTGTTTGGAAGGGTTCAGAGCGGGTGAGCCGTTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCCGCGTGCTTGGTGCCTTGGCTCCAAACCAATGCCCATTGCCCATGCTGTAGGTTCCCGTTTCCCTCTTAA

Coding sequence (CDS)

ATGGCCGGAATGTTGCCGGGAGTTGAATGCGCCAGAAGGCGGCGATTCCACCAGAGCATAACCTTATCGGACACGCCATCCACGGCGGCGAAATTAGGATCCACAAGAAGGCCGTCGTTCTGTTTGTACACGAGCAACCGTGATTTTCACATCACTTCATCATCGTCTTGTTCACAGCAACGGAGCTCATCCCATCAAGCTTACCAGAACGAGAAGCTTGGTGAAATTGCAAGAGAAGCTAAGGAACGGTTGGATGAGAGGTTAAGAACTCATAGAAAACCTGAAAATCCAAGGAAAAAGAGTGGAGAAGGGTTGAAGAACTCATTGGAAAAGAAAGAAAAAGCGAAGGGGAAAGAAGAATCAATAACGAAGAAGAGGTTCACTTGGGGGAAGCTGATGAAATGGAAAGCCTCGGAGCAGGAAGAGTGCGCCGTTTGTTTGGAAGGGTTCAGAGCGGGTGAGCCGTTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCCGCGTGCTTGGTGCCTTGGCTCCAAACCAATGCCCATTGCCCATGCTGTAGGTTCCCGTTTCCCTCTTAA

Protein sequence

MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQRSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEKAKGKEESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCPCCRFPFPS

Homology

[Close]

BLAST of Carg23020 vs. NCBI nr
Match: KAG7030344.1 (putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 382.1 bits (980), Expect = 2.8e-102
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60
           MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEKAKGKEE 120
           RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEKAKGKEE
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEKAKGKEE 120

Query: 121 SITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHC 180
           SITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHC
Sbjct: 121 SITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHC 180

Query: 181 PCCRFPFPS 190
           PCCRFPFPS
Sbjct: 181 PCCRFPFPS 189

[Close]

BLAST of Carg23020 vs. NCBI nr
Match: XP_022946687.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita moschata])

HSP 1 Score: 349.4 bits (895), Expect = 2.0e-92
Identity = 175/189 (92.59%), Postives = 175/189 (92.59%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60
           MAGMLPGVECARRRRFHQSIT SDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEKAKGKEE 120
           RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEG             KEE
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEG------------WKEE 120

Query: 121 SITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHC 180
           SITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHC
Sbjct: 121 SITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHC 177

Query: 181 PCCRFPFPS 190
           PCCRFPFPS
Sbjct: 181 PCCRFPFPS 177

[Close]

BLAST of Carg23020 vs. NCBI nr
Match: XP_023545449.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 333.6 bits (854), Expect = 1.1e-87
Identity = 169/190 (88.95%), Postives = 172/190 (90.53%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHIT-SSSSCSQ 60
           MAGMLPGVECARRRRFHQSIT SD+PSTAAKLGSTRRPSFCLYTSNRDFHIT SSSS SQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60

Query: 61  QRSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEKAKGKE 120
           QRSSS+QAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEG             KE
Sbjct: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEG------------SKE 120

Query: 121 ESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAH 180
           ESITKKRFTWGKLMKWKASEQEEC +CLEGFRAGEPLVHLPCAHKFHSACLVPWLQ NAH
Sbjct: 121 ESITKKRFTWGKLMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAH 178

Query: 181 CPCCRFPFPS 190
           CPCCRFPFPS
Sbjct: 181 CPCCRFPFPS 178

[Close]

BLAST of Carg23020 vs. NCBI nr
Match: XP_022999563.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita maxima])

HSP 1 Score: 314.7 bits (805), Expect = 5.4e-82
Identity = 160/187 (85.56%), Postives = 166/187 (88.77%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSC--S 60
           MAGMLPGVECARRRRFHQSIT SD+PSTAAKLGSTRRPSFCLYTSNRDFHITSSSS   S
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSRS 60

Query: 61  QQRSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEKAKGK 120
           QQRSSS+QAYQ EKLGEIAREAKERLDERL++HRKP+NPRKKSGEG             K
Sbjct: 61  QQRSSSNQAYQTEKLGEIAREAKERLDERLKSHRKPQNPRKKSGEG------------SK 120

Query: 121 EESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNA 180
           EESITKKRFTWGKLMKWKASEQ+ECAVCLEGFRAGEPLVHLPCAHKFHSAC VPWLQ N 
Sbjct: 121 EESITKKRFTWGKLMKWKASEQQECAVCLEGFRAGEPLVHLPCAHKFHSACFVPWLQANH 175

Query: 181 HCPCCRF 186
           HCPCCRF
Sbjct: 181 HCPCCRF 175

[Close]

BLAST of Carg23020 vs. NCBI nr
Match: XP_038891102.1 (probable E3 ubiquitin-protein ligase RHY1A [Benincasa hispida])

HSP 1 Score: 307.8 bits (787), Expect = 6.7e-80
Identity = 163/198 (82.32%), Postives = 169/198 (85.35%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60
           MAGMLPGVECARRRRFHQSI  SD+PSTAAK GSTRRPSFCLYTSNRDFH TSSSS SQ 
Sbjct: 1   MAGMLPGVECARRRRFHQSIASSDSPSTAAKSGSTRRPSFCLYTSNRDFHSTSSSS-SQH 60

Query: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGE---GLKNSLEK----KE 120
           RSSS+QAYQNEKLGEIAREAKERLDERLRTHRKPEN R+KS     G K S EK    KE
Sbjct: 61  RSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENSRRKSENEKVGWKGSSEKNGEEKE 120

Query: 121 KAKGKEES--ITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLV 180
           + KGKEES  ITKKRF WGKLMKW+ASEQEEC VCLE FR GEPLVHLPCAHKFHS CLV
Sbjct: 121 RTKGKEESIIITKKRFNWGKLMKWRASEQEECTVCLERFRVGEPLVHLPCAHKFHSTCLV 180

Query: 181 PWLQTNAHCPCCRFPFPS 190
           PWLQ N+HCPCCRFP PS
Sbjct: 181 PWLQANSHCPCCRFPIPS 197

[Close]

BLAST of Carg23020 vs. ExPASy Swiss-Prot
Match: Q852U6 (Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RHY1A PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 8.1e-12
Identity = 26/47 (55.32%), Postives = 33/47 (70.21%), Query Frame = 0


Query: 138 SEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCPCCR 185
           SE  +C++CLE F  G+ L+ LPC H FHS+CL PWL+    CPCCR
Sbjct: 198 SEMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCR 244

[Close]

BLAST of Carg23020 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 8.9e-11
Identity = 25/54 (46.30%), Postives = 34/54 (62.96%), Query Frame = 0


Query: 136 KASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCPCCRFPFPS 190
           K  E   C+VCL+    G     +PC HKFHS+C++PWL+ ++ CP CRF  PS
Sbjct: 192 KIEEVVSCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWLELHSSCPVCRFELPS 245

[Close]

BLAST of Carg23020 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.4e-10
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 138 SEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCPCCRFPFPS 190
           SE  +CAVC++ F  G  +  +PC H FH  CL+PWL+ +  CP CRF  P+
Sbjct: 211 SEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPT 262

[Close]

BLAST of Carg23020 vs. ExPASy Swiss-Prot
Match: Q9ULT6 (E3 ubiquitin-protein ligase ZNRF3 OS=Homo sapiens OX=9606 GN=ZNRF3 PE=1 SV=3)

HSP 1 Score: 60.5 bits (145), Expect = 2.4e-08
Identity = 30/77 (38.96%), Postives = 43/77 (55.84%), Query Frame = 0


Query: 108 SLEKKEKAKGKEESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHS 167
           +LEK E  K   +S  ++  + G L    +S   +CA+CLE +  GE L  +PC H+FH 
Sbjct: 258 ALEKMETRKFNSKSKGRREGSCGALDTLSSSSTSDCAICLEKYIDGEELRVIPCTHRFHR 317

Query: 168 ACLVPWLQTNAHCPCCR 185
            C+ PWL  +  CP CR
Sbjct: 318 KCVDPWLLQHHTCPHCR 334

[Close]

BLAST of Carg23020 vs. ExPASy Swiss-Prot
Match: A5WWA0 (E3 ubiquitin-protein ligase znrf3 OS=Danio rerio OX=7955 GN=znrf3 PE=1 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 4.1e-08
Identity = 30/77 (38.96%), Postives = 43/77 (55.84%), Query Frame = 0


Query: 108 SLEKKEKAKGKEESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHS 167
           +LEK E  K K +   ++  + G      +S   +CA+CLE +  GE L  +PCAH+FH 
Sbjct: 232 ALEKMETCKFKAKFKGQREASCGASDSVSSSSTSDCAICLEKYIDGEELRVIPCAHRFHK 291

Query: 168 ACLVPWLQTNAHCPCCR 185
            C+ PWL  +  CP CR
Sbjct: 292 KCVDPWLLQHHTCPHCR 308

[Close]

BLAST of Carg23020 vs. ExPASy TrEMBL
Match: A0A6J1G4D6 (probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC111450678 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 9.7e-93
Identity = 175/189 (92.59%), Postives = 175/189 (92.59%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60
           MAGMLPGVECARRRRFHQSIT SDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEKAKGKEE 120
           RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEG             KEE
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEG------------WKEE 120

Query: 121 SITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHC 180
           SITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHC
Sbjct: 121 SITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHC 177

Query: 181 PCCRFPFPS 190
           PCCRFPFPS
Sbjct: 181 PCCRFPFPS 177

[Close]

BLAST of Carg23020 vs. ExPASy TrEMBL
Match: A0A6J1KK21 (probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita maxima OX=3661 GN=LOC111493891 PE=4 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.6e-82
Identity = 160/187 (85.56%), Postives = 166/187 (88.77%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSC--S 60
           MAGMLPGVECARRRRFHQSIT SD+PSTAAKLGSTRRPSFCLYTSNRDFHITSSSS   S
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSRS 60

Query: 61  QQRSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEKAKGK 120
           QQRSSS+QAYQ EKLGEIAREAKERLDERL++HRKP+NPRKKSGEG             K
Sbjct: 61  QQRSSSNQAYQTEKLGEIAREAKERLDERLKSHRKPQNPRKKSGEG------------SK 120

Query: 121 EESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNA 180
           EESITKKRFTWGKLMKWKASEQ+ECAVCLEGFRAGEPLVHLPCAHKFHSAC VPWLQ N 
Sbjct: 121 EESITKKRFTWGKLMKWKASEQQECAVCLEGFRAGEPLVHLPCAHKFHSACFVPWLQANH 175

Query: 181 HCPCCRF 186
           HCPCCRF
Sbjct: 181 HCPCCRF 175

[Close]

BLAST of Carg23020 vs. ExPASy TrEMBL
Match: A0A6J1FHS8 (E3 ubiquitin-protein ligase ZNRF3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444265 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.7e-76
Identity = 155/189 (82.01%), Postives = 163/189 (86.24%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60
           MAGMLPGVECARRRRFHQSIT SD+ STAAKLGSTRR SFCLY SNRDFH+TSSS  SQQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSASTAAKLGSTRRSSFCLYASNRDFHLTSSS--SQQ 60

Query: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGE---GLKNSLEKKEKAKG 120
           R SS+ AYQ+EKLG+ AREAKERLDERLRTHRKPEN RKKS     GL     KKEKAKG
Sbjct: 61  RCSSNHAYQDEKLGQAAREAKERLDERLRTHRKPENSRKKSSSEEVGL-----KKEKAKG 120

Query: 121 KEESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTN 180
           KEE+ TKKRF WGKLMKWKASEQEEC VCLE FRA EPLVHLPCAHKFHSACLVPWLQ N
Sbjct: 121 KEETTTKKRFGWGKLMKWKASEQEECTVCLEKFRATEPLVHLPCAHKFHSACLVPWLQAN 180

Query: 181 AHCPCCRFP 187
           A+CPCCR+P
Sbjct: 181 AYCPCCRYP 182

[Close]

BLAST of Carg23020 vs. ExPASy TrEMBL
Match: A0A6J1HQI5 (probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465688 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 6.3e-76
Identity = 151/186 (81.18%), Postives = 160/186 (86.02%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60
           MAGMLPGVECARRRRFHQSIT S++ STAAKLGSTRR SFCLYTSNRDFH+TSSS  SQQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSS--SQQ 60

Query: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEKAKGKEE 120
           R SS+ AYQNEKLG+ AREAKERLDERLRTHRKPEN R KS    +    KKE  KGKEE
Sbjct: 61  RCSSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSS--EEVRLKKENVKGKEE 120

Query: 121 SITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHC 180
           + TKKRF+WGKLMKWKASEQEEC VCLE FRA EPLVHLPCAHKFHS CLVPWLQ NAHC
Sbjct: 121 TTTKKRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHC 180

Query: 181 PCCRFP 187
           PCCR+P
Sbjct: 181 PCCRYP 182

[Close]

BLAST of Carg23020 vs. ExPASy TrEMBL
Match: A0A0A0LG47 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 PE=4 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 6.5e-73
Identity = 153/197 (77.66%), Postives = 163/197 (82.74%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60
           MAGMLPGVECARRRRFHQSI  SD+P TA K GSTRRPSFCLYTSNRDFH+ SSSS SQQ
Sbjct: 1   MAGMLPGVECARRRRFHQSINSSDSPFTAGKSGSTRRPSFCLYTSNRDFHLISSSS-SQQ 60

Query: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSG--------EGLKNSLEKK 120
           RS ++QAYQN+KLGEIAREAKERLDERLRTHRKPEN RKK+            KN+ EKK
Sbjct: 61  RSLTNQAYQNDKLGEIAREAKERLDERLRTHRKPENSRKKNNNEEVKGKESREKNNGEKK 120

Query: 121 E--KAKGKEESITKKRFTWG-KLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSAC 180
           E    KGKEE  TKKRF+WG KLMKWKASEQEEC+VCLE FR  EPL+HLPCAHKFHS C
Sbjct: 121 EMKNTKGKEEE-TKKRFSWGRKLMKWKASEQEECSVCLERFRLSEPLLHLPCAHKFHSTC 180

Query: 181 LVPWLQTNAHCPCCRFP 187
           LVPWLQ NAHCPCCRFP
Sbjct: 181 LVPWLQANAHCPCCRFP 195

[Close]

BLAST of Carg23020 vs. TAIR 10
Match: AT2G15580.1 (RING/U-box superfamily protein )

HSP 1 Score: 146.7 bits (369), Expect = 1.8e-35
Identity = 93/198 (46.97%), Postives = 119/198 (60.10%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFH---QSITLSDTPSTAAKLGS--TRRPSFCLYTSNRDFHITSSS 60
           MAGMLPGVECARRRRFH     I  S+T S AA  G   TRRPSF LYT+N + H    S
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61  SCSQQ-RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLKNSLEKKEK 120
              +  R+ S+    +EKL   A+EAK+RL++RLR   +     K  G        K E+
Sbjct: 61  FSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLRIPPRTRQNGKDKG-------NKLEQ 120

Query: 121 AKGK-----EESITKKRFTWGKLMKW---KASEQEECAVCLEGFRAGEPLVHLPCAHKFH 180
            KGK        +   + + G+LM+W   +  EQ++CA+CL+ F+ GE LVHLPCAHKFH
Sbjct: 121 GKGKPLGDLPTEVVGLKKSRGRLMEWFKRRVREQQDCAICLDRFKKGETLVHLPCAHKFH 180

Query: 181 SACLVPWLQTNAHCPCCR 185
           S CL+PWL TN +CP CR
Sbjct: 181 SICLLPWLDTNVYCPYCR 191

[Close]

BLAST of Carg23020 vs. TAIR 10
Match: AT2G15580.2 (RING/U-box superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.0e-13
Identity = 55/112 (49.11%), Postives = 68/112 (60.71%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFH---QSITLSDTPSTAAKLGS--TRRPSFCLYTSNRDFHITSSS 60
           MAGMLPGVECARRRRFH     I  S+T S AA  G   TRRPSF LYT+N + H    S
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61  SCSQQ-RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEGLK 107
              +  R+ S+    +EKL   A+EAK+RL++RLR       PR  SG+ +K
Sbjct: 61  FSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLRI-----PPRTSSGKMVK 107

[Close]

BLAST of Carg23020 vs. TAIR 10
Match: AT1G49850.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 5.7e-13
Identity = 26/47 (55.32%), Postives = 33/47 (70.21%), Query Frame = 0


Query: 138 SEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCPCCR 185
           SE  +C++CLE F  G+ L+ LPC H FHS+CL PWL+    CPCCR
Sbjct: 198 SEMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCR 244

[Close]

BLAST of Carg23020 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 6.3e-12
Identity = 26/54 (48.15%), Postives = 34/54 (62.96%), Query Frame = 0


Query: 136 KASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCPCCRFPFPS 190
           K  E  +C+VCL+ F  G     +PC HKFH  C+VPWL+ ++ CP CRF  PS
Sbjct: 252 KIMEPLQCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWLELHSSCPVCRFELPS 305

[Close]

BLAST of Carg23020 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.4e-11
Identity = 25/54 (46.30%), Postives = 35/54 (64.81%), Query Frame = 0


Query: 136 KASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCPCCRFPFPS 190
           K  E  +C+VCL+ F  G     +PC HKFHS CL+PWL+ ++ CP CR+  P+
Sbjct: 216 KIEETLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPA 269

The following BLAST results are available for this feature:

BLAST of Carg23020 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030344.1	2.8e-102	100.00	putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subs...	[more]
XP_022946687.1	2.0e-92	92.59	probable E3 ubiquitin-protein ligase RHY1A [Cucurbita moschata]	[more]
XP_023545449.1	1.1e-87	88.95	probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo]	[more]
XP_022999563.1	5.4e-82	85.56	probable E3 ubiquitin-protein ligase RHY1A [Cucurbita maxima]	[more]
XP_038891102.1	6.7e-80	82.32	probable E3 ubiquitin-protein ligase RHY1A [Benincasa hispida]	[more]

BLAST of Carg23020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q852U6	8.1e-12	55.32	Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q6AVN2	8.9e-11	46.30	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8LPN7	3.4e-10	46.15	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q9ULT6	2.4e-08	38.96	E3 ubiquitin-protein ligase ZNRF3 OS=Homo sapiens OX=9606 GN=ZNRF3 PE=1 SV=3	[more]
A5WWA0	4.1e-08	38.96	E3 ubiquitin-protein ligase znrf3 OS=Danio rerio OX=7955 GN=znrf3 PE=1 SV=2	[more]

BLAST of Carg23020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4D6	9.7e-93	92.59	probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1KK21	2.6e-82	85.56	probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1FHS8	1.7e-76	82.01	E3 ubiquitin-protein ligase ZNRF3-like isoform X1 OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1HQI5	6.3e-76	81.18	probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=366...	[more]
A0A0A0LG47	6.5e-73	77.66	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 P...	[more]

BLAST of Carg23020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15580.1	1.8e-35	46.97	RING/U-box superfamily protein	[more]
AT2G15580.2	2.0e-13	49.11	RING/U-box superfamily protein	[more]
AT1G49850.1	5.7e-13	55.32	RING/U-box superfamily protein	[more]
AT5G56340.1	6.3e-12	48.15	RING/U-box superfamily protein	[more]
AT1G55530.1	1.4e-11	46.30	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 143..183 e-value: 3.8E-5 score: 33.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 142..184 e-value: 2.6E-11 score: 43.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 143..184 score: 12.267982
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 89..189 e-value: 2.4E-18 score: 67.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 86..117
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..70
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..73
None	No IPR available	PANTHER	PTHR22765	RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING	coord: 1..184
None	No IPR available	PANTHER	PTHR22765:SF204	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..184
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 142..184 e-value: 5.37538E-18 score: 71.5641
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 129..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23020-RA	Carg23020-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
molecular_function	GO:0061630	ubiquitin protein ligase activity