Carg22968 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22968
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncold-regulated 413 plasma membrane protein 2-like
LocationCarg_Chr02: 1909782 .. 1911679 (-)
RNA-Seq ExpressionCarg22968
SyntenyCarg22968
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTTCTTCTTCTTCTTATACTTCTGTAATTTCATCAGATCGGAAGATCAAAGCGGCCGTACTGAAATGGTGAAACCGAGCTACTTGAAAATGTTGACGGATTCTGACGTTTCCGATCTTATCTCCTCTGATCTTCGGGAACTTCGTAACGCTGGTAAAAAGCTTGCTACACACGTCGTTAAGCTTAGTGCTGTGGGTTTTGGCGCTACTTTCCTCCAATGGATTGCTTCCTTTGCTGCTATGTATGTTTCTCTCTCTCATATGGCTTTATTCCTGCTTCATTTAGCGATGTTGTTGTTTGTGGTTCGTTGCTGACGGTTGATTGTTTTAGAGGAAATTTTAGTTGTGAGAGGCTTCTGTTTCGAGGAATAGTTTGTGTTTAGTGATCGATTTTCTGTTTAACTGAAGTGATTCCTTTCTTGAATTAGATATTGAACTGATTGAGGAATATGAGAAGGATTTAGTTAGGGAAATGATTACTGTAACCAAATGTTCGTTTTCGTTTTGATATCTGCTTGATCTTCTTTTAGGTTTTTCTGGATCATTTCGATTCGACTTCGATTCCTTTCTGTTCTTATTATTGTACACTTTTGCAACTTGAATTTTTTTTGTGAGTGCAGTTACTTGCTGATTTTGGATCGAACGAACTGGAAGACGAATATACTTACCTCATTGTTGATTCCGTACATTTTCTTTAGTCTTCCTGGTTTGATTTTCGGTTTTCTCAGGTGATCATCTCTATAGCAATTGGCTCCGAATGAAGCTCCCGGAATTACGCTTCTGTCGTCTCTAACTAAATTGCTTTCTCTTGCAGGGGAGAGGTCGGAAAGTGGATCGCCGCCATTGCTGTAGTGCTGCGTCTCTTCTTCCCACGACGATTCCCAGGTAGTTTTCAGGACTCGACTTGATTACAGAAATTACAGAATTCAGTTTCAGTTTATGAACTACAAAATATTCTGGATGATGAGTTTCTTGAAATAGATTTAGAGCCACCAGTAGTTTTGATTACTCTCTTCTTCCTTCTGTTTCATTTTGGATGACTGCCACAATGACTAGTTTTATATTCTCCTTTTCATTTCTTGCTAAGAACTATTGAAATCTTAACAATGAAGAAGAATCATTATCAAACTGTTTAACATCTTTGAGCCTGTAAAAAGTTAGATAGATCTTAAAGCATCAAAATGATCAAAAAGTTTAGTTGAAGTATACTACCATTAGGTATTTAACATACCTTCGAACTTTTTGTAAGGAATCCTTCCCAACAATCCTCAATAATCCTCCCCTCGAACAAAGTACATTATAGAGGCTTCCCTGATGCTTATGGAGCTCTCGAAAAGCCTCTCTTTAATCGAGGCTACATTAGCAAATGAGGGACTCCTTCCCAACGATCGGCAGCACTTTCACACTTGCATTCCAATTTCGAAGGTAATATGATTTGCTTAGTTTGAAGGAACAAACAACATCCCAAACCTCATCCATAATCAAATATCCTCCAATCTAATAGCTTAAGCTTTCAGTAAATAGAAATTTCACATCATGTTGAAGTATAGTATGTCCTGTCTAGTTTGCAAGCTTAAGAGCCAAAAGAATTAGATGTCAAAACTGAATTATTGCACTCCCTTCTCTCATTGCCTGGAAATACAGATTGGCTTGAATTGCCTGGCGCTTTGATACTTCTAATTGTGGTGGCTCCGGGGTTATTTGCGAGCACCATAAGGGGCGACGCCATCGGAGAAGTGATATGTCTGATAATATCATGCTATTTGCTGCAAGAACACATCAGAGCCTCTGGTGGATTCAGAAACTCCTTCACCAAAGCCAATGGCATTTCAAACACCATTGGCATTGTCCTTCTCTTTGTTTTTCCTGTCTGGGCTCTGTTACTTCGCGTGCTTTGA

mRNA sequence

TTCTTCTTCTTCTTCTTATACTTCTGTAATTTCATCAGATCGGAAGATCAAAGCGGCCGTACTGAAATGGTGAAACCGAGCTACTTGAAAATGTTGACGGATTCTGACGTTTCCGATCTTATCTCCTCTGATCTTCGGGAACTTCGTAACGCTGGTAAAAAGCTTGCTACACACGTCGTTAAGCTTAGTGCTGTGGGTTTTGGCGCTACTTTCCTCCAATGGATTGCTTCCTTTGCTGCTATTTACTTGCTGATTTTGGATCGAACGAACTGGAAGACGAATATACTTACCTCATTGTTGATTCCGTACATTTTCTTTAGTCTTCCTGGTTTGATTTTCGGTTTTCTCAGGGGAGAGGTCGGAAAGTGGATCGCCGCCATTGCTGTAGTGCTGCGTCTCTTCTTCCCACGACGATTCCCAGATTGGCTTGAATTGCCTGGCGCTTTGATACTTCTAATTGTGGTGGCTCCGGGGTTATTTGCGAGCACCATAAGGGGCGACGCCATCGGAGAAGTGATATGTCTGATAATATCATGCTATTTGCTGCAAGAACACATCAGAGCCTCTGGTGGATTCAGAAACTCCTTCACCAAAGCCAATGGCATTTCAAACACCATTGGCATTGTCCTTCTCTTTGTTTTTCCTGTCTGGGCTCTGTTACTTCGCGTGCTTTGA

Coding sequence (CDS)

ATGGTGAAACCGAGCTACTTGAAAATGTTGACGGATTCTGACGTTTCCGATCTTATCTCCTCTGATCTTCGGGAACTTCGTAACGCTGGTAAAAAGCTTGCTACACACGTCGTTAAGCTTAGTGCTGTGGGTTTTGGCGCTACTTTCCTCCAATGGATTGCTTCCTTTGCTGCTATTTACTTGCTGATTTTGGATCGAACGAACTGGAAGACGAATATACTTACCTCATTGTTGATTCCGTACATTTTCTTTAGTCTTCCTGGTTTGATTTTCGGTTTTCTCAGGGGAGAGGTCGGAAAGTGGATCGCCGCCATTGCTGTAGTGCTGCGTCTCTTCTTCCCACGACGATTCCCAGATTGGCTTGAATTGCCTGGCGCTTTGATACTTCTAATTGTGGTGGCTCCGGGGTTATTTGCGAGCACCATAAGGGGCGACGCCATCGGAGAAGTGATATGTCTGATAATATCATGCTATTTGCTGCAAGAACACATCAGAGCCTCTGGTGGATTCAGAAACTCCTTCACCAAAGCCAATGGCATTTCAAACACCATTGGCATTGTCCTTCTCTTTGTTTTTCCTGTCTGGGCTCTGTTACTTCGCGTGCTTTGA

Protein sequence

MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIYLLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDWLELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGISNTIGIVLLFVFPVWALLLRVL
Homology
BLAST of Carg22968 vs. NCBI nr
Match: XP_023532118.1 (cold-regulated 413 plasma membrane protein 2-like [Cucurbita pepo subsp. pepo] >KAG7035110.1 Cold-regulated plasma membrane protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 391.0 bits (1003), Expect = 6.4e-105
Identity = 202/202 (100.00%), Postives = 202/202 (100.00%), Query Frame = 0

Query: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60
           MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY
Sbjct: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60

Query: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120
           LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW
Sbjct: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120

Query: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180
           LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI
Sbjct: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180

Query: 181 SNTIGIVLLFVFPVWALLLRVL 203
           SNTIGIVLLFVFPVWALLLRVL
Sbjct: 181 SNTIGIVLLFVFPVWALLLRVL 202

BLAST of Carg22968 vs. NCBI nr
Match: XP_022948197.1 (cold-regulated 413 plasma membrane protein 2-like [Cucurbita moschata])

HSP 1 Score: 388.3 bits (996), Expect = 4.1e-104
Identity = 201/202 (99.50%), Postives = 201/202 (99.50%), Query Frame = 0

Query: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60
           MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY
Sbjct: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60

Query: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120
           LLILDRTNWKTNILTSLLIPYIFFSLP LIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW
Sbjct: 61  LLILDRTNWKTNILTSLLIPYIFFSLPDLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120

Query: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180
           LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI
Sbjct: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180

Query: 181 SNTIGIVLLFVFPVWALLLRVL 203
           SNTIGIVLLFVFPVWALLLRVL
Sbjct: 181 SNTIGIVLLFVFPVWALLLRVL 202

BLAST of Carg22968 vs. NCBI nr
Match: XP_023007529.1 (cold-regulated 413 plasma membrane protein 2-like [Cucurbita maxima])

HSP 1 Score: 387.9 bits (995), Expect = 5.4e-104
Identity = 200/202 (99.01%), Postives = 200/202 (99.01%), Query Frame = 0

Query: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60
           MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY
Sbjct: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60

Query: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120
           LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIA IAVVLRLFFPRRFPDW
Sbjct: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAVIAVVLRLFFPRRFPDW 120

Query: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180
           LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI
Sbjct: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180

Query: 181 SNTIGIVLLFVFPVWALLLRVL 203
           SNTIGIVLLFVFPVW LLLRVL
Sbjct: 181 SNTIGIVLLFVFPVWVLLLRVL 202

BLAST of Carg22968 vs. NCBI nr
Match: KAG6605109.1 (Cold-regulated 413 plasma membrane protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 372.1 bits (954), Expect = 3.1e-99
Identity = 192/194 (98.97%), Postives = 194/194 (100.00%), Query Frame = 0

Query: 9   MLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIYLLILDRTN 68
           MLTDSDVSDLISSDLRELR+AGK+LATHVVKLSAVGFGATFLQWIASFAAIYLLILDRTN
Sbjct: 1   MLTDSDVSDLISSDLRELRDAGKRLATHVVKLSAVGFGATFLQWIASFAAIYLLILDRTN 60

Query: 69  WKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDWLELPGALI 128
           WKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDWLELPGALI
Sbjct: 61  WKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDWLELPGALI 120

Query: 129 LLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGISNTIGIVL 188
           LLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGISNTIGIVL
Sbjct: 121 LLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGISNTIGIVL 180

Query: 189 LFVFPVWALLLRVL 203
           LFVFPVWALLLRVL
Sbjct: 181 LFVFPVWALLLRVL 194

BLAST of Carg22968 vs. NCBI nr
Match: XP_038902783.1 (cold-regulated 413 plasma membrane protein 2-like [Benincasa hispida])

HSP 1 Score: 359.4 bits (921), Expect = 2.1e-95
Identity = 183/202 (90.59%), Postives = 190/202 (94.06%), Query Frame = 0

Query: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60
           MVKP+YLKM+TDSD +DLISSDLREL NA KKLATH VKL A GF A+FLQWIASFAAIY
Sbjct: 1   MVKPNYLKMVTDSDAADLISSDLRELGNAAKKLATHAVKLCASGFAASFLQWIASFAAIY 60

Query: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120
           LLILDRTNWKTNILTSLLIPYIFFSLPG+IFGFLRGE GKW+A IAVVLRLFFPRRFPDW
Sbjct: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGVIFGFLRGEFGKWVAVIAVVLRLFFPRRFPDW 120

Query: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180
           LELPGALILLIVVAP LFA TIRGDAIGE ICLIISCYLLQEHIRASGGFRNSFTKANGI
Sbjct: 121 LELPGALILLIVVAPSLFAHTIRGDAIGEAICLIISCYLLQEHIRASGGFRNSFTKANGI 180

Query: 181 SNTIGIVLLFVFPVWALLLRVL 203
           SNTIGIVLLFVFPVWAL+LRVL
Sbjct: 181 SNTIGIVLLFVFPVWALVLRVL 202

BLAST of Carg22968 vs. ExPASy Swiss-Prot
Match: Q9SVL6 (Cold-regulated 413 plasma membrane protein 2 OS=Arabidopsis thaliana OX=3702 GN=COR413PM2 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 4.1e-70
Identity = 135/203 (66.50%), Postives = 163/203 (80.30%), Query Frame = 0

Query: 1   MVKPSYLKMLTDS-DVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAI 60
           M +  YL M TD  D   L++SD+ EL+ A KKL + V KL  +GFG +FL+++ASFAAI
Sbjct: 1   MGRMDYLAMKTDDVDTVALVNSDMEELKVAAKKLFSDVSKLGGLGFGVSFLKFLASFAAI 60

Query: 61  YLLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPD 120
           YLLILDRTNWKT +LTSLLIPYIF SLP +IF FL G+VGKWIA +AVVLRLFFP+ FPD
Sbjct: 61  YLLILDRTNWKTKMLTSLLIPYIFLSLPSVIFNFLSGDVGKWIAFVAVVLRLFFPKHFPD 120

Query: 121 WLELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANG 180
           WLE+PG+LILL+VV+P   A  IRG  IG VI L I CYLLQEHIRASGGFRNSFT+  G
Sbjct: 121 WLEMPGSLILLLVVSPHFLAHHIRGTWIGTVISLFIGCYLLQEHIRASGGFRNSFTQPRG 180

Query: 181 ISNTIGIVLLFVFPVWALLLRVL 203
           +SNT+GI+LL V+PVWAL++RV+
Sbjct: 181 VSNTLGIILLLVYPVWALIVRVM 203

BLAST of Carg22968 vs. ExPASy Swiss-Prot
Match: Q9XIM7 (Cold-regulated 413 plasma membrane protein 1 OS=Arabidopsis thaliana OX=3702 GN=COR413PM1 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 4.6e-66
Identity = 125/181 (69.06%), Postives = 145/181 (80.11%), Query Frame = 0

Query: 18  LISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIYLLILDRTNWKTNILTSL 77
           +I SD  EL  A K LATH   L+ +GFG + L+W+AS AAIYLL+LDRTNWKTN+LTSL
Sbjct: 16  MIGSDFNELTIAAKNLATHAFTLTGLGFGTSVLEWVASIAAIYLLVLDRTNWKTNMLTSL 75

Query: 78  LIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDWLELPGALILLIVVAPGL 137
           LIPYIFFSLP LIFG  RGE+GKWIA +AVV++LFFP+   ++LELP AL+LL VVAP L
Sbjct: 76  LIPYIFFSLPSLIFGIFRGEIGKWIAFVAVVVQLFFPKHAREYLELPVALVLLAVVAPNL 135

Query: 138 FASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGISNTIGIVLLFVFPVWAL 197
            A T R   IG  ICL I CYLLQEHIRASGGFRN+FTKANGISNT+GI+ L VFPVWAL
Sbjct: 136 IAGTFRDSWIGLAICLGIGCYLLQEHIRASGGFRNAFTKANGISNTVGIICLVVFPVWAL 195

Query: 198 L 199
           +
Sbjct: 196 I 196

BLAST of Carg22968 vs. ExPASy Swiss-Prot
Match: O23164 (Cold-regulated 413 plasma membrane protein 4 OS=Arabidopsis thaliana OX=3702 GN=At4g37220 PE=2 SV=2)

HSP 1 Score: 246.5 bits (628), Expect = 2.6e-64
Identity = 118/201 (58.71%), Postives = 152/201 (75.62%), Query Frame = 0

Query: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60
           M +  +L M T+ + ++LI+SD+ E   A KKL   V  L  VGFG + LQW AS  AIY
Sbjct: 1   MGRGEFLAMKTEENAANLINSDMNEFVAAAKKLVKDVGMLGGVGFGTSVLQWAASIFAIY 60

Query: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120
           LLILDRTNWKT +LT+LL+PYIFF+LP +IF F  G+ GKWIA IA+++RLFFP+ FP+W
Sbjct: 61  LLILDRTNWKTKMLTTLLVPYIFFTLPSVIFQFFSGDFGKWIALIAIIVRLFFPKEFPEW 120

Query: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180
           LE+P ALIL++VV+P L A T+R   +G VICL+I+CYL  EHI+ASGGF+NSFT+ NGI
Sbjct: 121 LEIPVALILIVVVSPSLIAWTLRESWVGAVICLVIACYLFHEHIKASGGFKNSFTQKNGI 180

Query: 181 SNTIGIVLLFVFPVWALLLRV 202
           SNTIGIV L V+PVW +   +
Sbjct: 181 SNTIGIVALLVYPVWTIFFHI 201

BLAST of Carg22968 vs. ExPASy Swiss-Prot
Match: O64834 (Cold-regulated 413 plasma membrane protein 3 OS=Arabidopsis thaliana OX=3702 GN=At2g23680 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.8e-33
Identity = 74/150 (49.33%), Postives = 103/150 (68.67%), Query Frame = 0

Query: 49  FLQWIASFAAIYLLILDRTNWK--TNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIA 108
           FL+W+AS  A++L+ILD+T WK   NI+ SLL PY+F SLP +IF  LR  VGKWIA + 
Sbjct: 30  FLRWLASIVAVFLMILDQTKWKYSNNIMASLLAPYLFSSLPIVIFQVLRNGVGKWIALLT 89

Query: 109 VVLRLFFPRRFPDWLELPGALILLIVVAPGLFASTIRGDA--IGEVICLIISCYLLQEHI 168
           V+LRLF P  F + LE+PGA ILLIVV P    +  R D    G  +CL+ S YL+ +H 
Sbjct: 90  VILRLFLPNHFHESLEIPGATILLIVVTPSDIGAIFRDDLRYTGGDVCLLTSFYLINKHT 149

Query: 169 RASGGFRNSFTKANGISNTIGIVLLFVFPV 195
           +A GG +NSFT+ + ++ +I + +LFV+P+
Sbjct: 150 KACGGIKNSFTQKDKVTYSICLWILFVYPI 179

BLAST of Carg22968 vs. ExPASy Swiss-Prot
Match: Q94AL8 (Cold-regulated 413 inner membrane protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=COR413IM1 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.3e-12
Identity = 55/192 (28.65%), Postives = 97/192 (50.52%), Query Frame = 0

Query: 7   LKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIYLLILDR 66
           L++  D   +  +S+ + + R  G  +  +   +S     A  LQWI++ + + L++   
Sbjct: 47  LRLSGDRQRTATVSTRVEKRRKRGSSVVCYAAPIS-----ANSLQWISTISCLALMLAR- 106

Query: 67  TNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDWLELPGA 126
               T I  S+++P      P  I  +++GE G W A +A++ RLFF   FP  LELP  
Sbjct: 107 ---GTGIHKSVVVPLFALHAPSSIVAWIKGEYGVWAAFLALIARLFF--TFPGELELPFI 166

Query: 127 LILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGISNTIGI 186
            +LL++VAP      IRG   G +I + IS +L  +H   +G    ++ K + ++ T+ I
Sbjct: 167 ALLLVIVAP-YQVMNIRGKQEGAIIAIAISGFLAFQHFSRAGSLEKAYEKGSVLA-TVAI 225

Query: 187 VLLFVFPVWALL 199
           + + V  +  LL
Sbjct: 227 IGVTVVSLLLLL 225

BLAST of Carg22968 vs. ExPASy TrEMBL
Match: A0A6J1G8J0 (cold-regulated 413 plasma membrane protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111451846 PE=3 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.0e-104
Identity = 201/202 (99.50%), Postives = 201/202 (99.50%), Query Frame = 0

Query: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60
           MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY
Sbjct: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60

Query: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120
           LLILDRTNWKTNILTSLLIPYIFFSLP LIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW
Sbjct: 61  LLILDRTNWKTNILTSLLIPYIFFSLPDLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120

Query: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180
           LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI
Sbjct: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180

Query: 181 SNTIGIVLLFVFPVWALLLRVL 203
           SNTIGIVLLFVFPVWALLLRVL
Sbjct: 181 SNTIGIVLLFVFPVWALLLRVL 202

BLAST of Carg22968 vs. ExPASy TrEMBL
Match: A0A6J1L568 (cold-regulated 413 plasma membrane protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111499993 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.6e-104
Identity = 200/202 (99.01%), Postives = 200/202 (99.01%), Query Frame = 0

Query: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60
           MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY
Sbjct: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60

Query: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120
           LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIA IAVVLRLFFPRRFPDW
Sbjct: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAVIAVVLRLFFPRRFPDW 120

Query: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180
           LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI
Sbjct: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180

Query: 181 SNTIGIVLLFVFPVWALLLRVL 203
           SNTIGIVLLFVFPVW LLLRVL
Sbjct: 181 SNTIGIVLLFVFPVWVLLLRVL 202

BLAST of Carg22968 vs. ExPASy TrEMBL
Match: A0A1S3CAX8 (cold-regulated 413 plasma membrane protein 2-like OS=Cucumis melo OX=3656 GN=LOC103498423 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 8.4e-95
Identity = 181/202 (89.60%), Postives = 190/202 (94.06%), Query Frame = 0

Query: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60
           MVKP++LKM+TDSD +DLISSDLREL NA +KLATH VKL A GF A+FLQWIASFAAIY
Sbjct: 1   MVKPNHLKMVTDSDAADLISSDLRELGNAARKLATHAVKLGASGFTASFLQWIASFAAIY 60

Query: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120
           LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGE GKW+A IAVVLRLFFPRRFPDW
Sbjct: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEFGKWVAVIAVVLRLFFPRRFPDW 120

Query: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180
           LELPGALILLIVVAP LFASTIR DAIGE ICLIISCYLLQEHIRASGGFRNSFTKANGI
Sbjct: 121 LELPGALILLIVVAPSLFASTIRNDAIGEAICLIISCYLLQEHIRASGGFRNSFTKANGI 180

Query: 181 SNTIGIVLLFVFPVWALLLRVL 203
           SNTIGI+LLFVFPVWAL+LRVL
Sbjct: 181 SNTIGIILLFVFPVWALVLRVL 202

BLAST of Carg22968 vs. ExPASy TrEMBL
Match: A0A5A7TUZ7 (Cold-regulated 413 plasma membrane protein 2-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G005140 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.1e-94
Identity = 180/202 (89.11%), Postives = 190/202 (94.06%), Query Frame = 0

Query: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60
           MVKP++LKM+TDSD +DLISSDLREL NA +KLATH VKL A GF A+FLQWIASFAAIY
Sbjct: 1   MVKPNHLKMVTDSDAADLISSDLRELGNAARKLATHAVKLGASGFTASFLQWIASFAAIY 60

Query: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120
           LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGE GKW+A IAVVLRLFFPRRFPDW
Sbjct: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEFGKWVAVIAVVLRLFFPRRFPDW 120

Query: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180
           LELPGALILLIVVAP LFAST+R DAIGE ICLIISCYLLQEHIRASGGFRNSFTKANGI
Sbjct: 121 LELPGALILLIVVAPSLFASTVRNDAIGEAICLIISCYLLQEHIRASGGFRNSFTKANGI 180

Query: 181 SNTIGIVLLFVFPVWALLLRVL 203
           SNTIGI+LLFVFPVWAL+LRVL
Sbjct: 181 SNTIGIILLFVFPVWALVLRVL 202

BLAST of Carg22968 vs. ExPASy TrEMBL
Match: A0A6J1FVU6 (cold-regulated 413 plasma membrane protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111448574 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 5.5e-94
Identity = 179/202 (88.61%), Postives = 190/202 (94.06%), Query Frame = 0

Query: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60
           MVKPSYLKM+TDSD +DLIS+DLREL NA KKLATH V+L A GF A FLQW+ASFAAIY
Sbjct: 1   MVKPSYLKMVTDSDAADLISNDLRELGNAAKKLATHAVQLGASGFPAAFLQWLASFAAIY 60

Query: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120
           LLILDRTNWKTNILTSLLIPYIFFSLPG+IF FLRGE GKW+AAIAVVLRLFFPRRFPDW
Sbjct: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGVIFFFLRGEFGKWVAAIAVVLRLFFPRRFPDW 120

Query: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180
           LELPGALILLIVVAPGLFAST+R DAIGE ICLIISCYLLQEHIRASGGFRNSFTKANGI
Sbjct: 121 LELPGALILLIVVAPGLFASTMRNDAIGEAICLIISCYLLQEHIRASGGFRNSFTKANGI 180

Query: 181 SNTIGIVLLFVFPVWALLLRVL 203
           SNTIGI+LLFVFPVWAL++RVL
Sbjct: 181 SNTIGIILLFVFPVWALVVRVL 202

BLAST of Carg22968 vs. TAIR 10
Match: AT3G50830.1 (cold-regulated 413-plasma membrane 2 )

HSP 1 Score: 265.8 bits (678), Expect = 2.9e-71
Identity = 135/203 (66.50%), Postives = 163/203 (80.30%), Query Frame = 0

Query: 1   MVKPSYLKMLTDS-DVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAI 60
           M +  YL M TD  D   L++SD+ EL+ A KKL + V KL  +GFG +FL+++ASFAAI
Sbjct: 1   MGRMDYLAMKTDDVDTVALVNSDMEELKVAAKKLFSDVSKLGGLGFGVSFLKFLASFAAI 60

Query: 61  YLLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPD 120
           YLLILDRTNWKT +LTSLLIPYIF SLP +IF FL G+VGKWIA +AVVLRLFFP+ FPD
Sbjct: 61  YLLILDRTNWKTKMLTSLLIPYIFLSLPSVIFNFLSGDVGKWIAFVAVVLRLFFPKHFPD 120

Query: 121 WLELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANG 180
           WLE+PG+LILL+VV+P   A  IRG  IG VI L I CYLLQEHIRASGGFRNSFT+  G
Sbjct: 121 WLEMPGSLILLLVVSPHFLAHHIRGTWIGTVISLFIGCYLLQEHIRASGGFRNSFTQPRG 180

Query: 181 ISNTIGIVLLFVFPVWALLLRVL 203
           +SNT+GI+LL V+PVWAL++RV+
Sbjct: 181 VSNTLGIILLLVYPVWALIVRVM 203

BLAST of Carg22968 vs. TAIR 10
Match: AT2G15970.1 (cold regulated 413 plasma membrane 1 )

HSP 1 Score: 252.3 bits (643), Expect = 3.3e-67
Identity = 125/181 (69.06%), Postives = 145/181 (80.11%), Query Frame = 0

Query: 18  LISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIYLLILDRTNWKTNILTSL 77
           +I SD  EL  A K LATH   L+ +GFG + L+W+AS AAIYLL+LDRTNWKTN+LTSL
Sbjct: 16  MIGSDFNELTIAAKNLATHAFTLTGLGFGTSVLEWVASIAAIYLLVLDRTNWKTNMLTSL 75

Query: 78  LIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDWLELPGALILLIVVAPGL 137
           LIPYIFFSLP LIFG  RGE+GKWIA +AVV++LFFP+   ++LELP AL+LL VVAP L
Sbjct: 76  LIPYIFFSLPSLIFGIFRGEIGKWIAFVAVVVQLFFPKHAREYLELPVALVLLAVVAPNL 135

Query: 138 FASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGISNTIGIVLLFVFPVWAL 197
            A T R   IG  ICL I CYLLQEHIRASGGFRN+FTKANGISNT+GI+ L VFPVWAL
Sbjct: 136 IAGTFRDSWIGLAICLGIGCYLLQEHIRASGGFRNAFTKANGISNTVGIICLVVFPVWAL 195

Query: 198 L 199
           +
Sbjct: 196 I 196

BLAST of Carg22968 vs. TAIR 10
Match: AT4G37220.1 (Cold acclimation protein WCOR413 family )

HSP 1 Score: 246.5 bits (628), Expect = 1.8e-65
Identity = 118/201 (58.71%), Postives = 152/201 (75.62%), Query Frame = 0

Query: 1   MVKPSYLKMLTDSDVSDLISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIY 60
           M +  +L M T+ + ++LI+SD+ E   A KKL   V  L  VGFG + LQW AS  AIY
Sbjct: 1   MGRGEFLAMKTEENAANLINSDMNEFVAAAKKLVKDVGMLGGVGFGTSVLQWAASIFAIY 60

Query: 61  LLILDRTNWKTNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDW 120
           LLILDRTNWKT +LT+LL+PYIFF+LP +IF F  G+ GKWIA IA+++RLFFP+ FP+W
Sbjct: 61  LLILDRTNWKTKMLTTLLVPYIFFTLPSVIFQFFSGDFGKWIALIAIIVRLFFPKEFPEW 120

Query: 121 LELPGALILLIVVAPGLFASTIRGDAIGEVICLIISCYLLQEHIRASGGFRNSFTKANGI 180
           LE+P ALIL++VV+P L A T+R   +G VICL+I+CYL  EHI+ASGGF+NSFT+ NGI
Sbjct: 121 LEIPVALILIVVVSPSLIAWTLRESWVGAVICLVIACYLFHEHIKASGGFKNSFTQKNGI 180

Query: 181 SNTIGIVLLFVFPVWALLLRV 202
           SNTIGIV L V+PVW +   +
Sbjct: 181 SNTIGIVALLVYPVWTIFFHI 201

BLAST of Carg22968 vs. TAIR 10
Match: AT2G15970.2 (cold regulated 413 plasma membrane 1 )

HSP 1 Score: 176.4 bits (446), Expect = 2.3e-44
Identity = 88/136 (64.71%), Postives = 104/136 (76.47%), Query Frame = 0

Query: 18  LISSDLRELRNAGKKLATHVVKLSAVGFGATFLQWIASFAAIYLLILDRTNWKTNILTSL 77
           +I SD  EL  A K LATH   L+ +GFG + L+W+AS AAIYLL+LDRTNWKTN+LTSL
Sbjct: 16  MIGSDFNELTIAAKNLATHAFTLTGLGFGTSVLEWVASIAAIYLLVLDRTNWKTNMLTSL 75

Query: 78  LIPYIFFSLPGLIFGFLRGEVGKWIAAIAVVLRLFFPRRFPDWLELPGALILLIVVAPGL 137
           LIPYIFFSLP LIFG  RGE+GKWIA +AVV++LFFP+   ++LELP AL+LL VVAP L
Sbjct: 76  LIPYIFFSLPSLIFGIFRGEIGKWIAFVAVVVQLFFPKHAREYLELPVALVLLAVVAPNL 135

Query: 138 FASTIRGDAIGEVICL 154
            A T R   IG  ICL
Sbjct: 136 IAGTFRDSWIGLAICL 151

BLAST of Carg22968 vs. TAIR 10
Match: AT2G23680.1 (Cold acclimation protein WCOR413 family )

HSP 1 Score: 144.1 bits (362), Expect = 1.3e-34
Identity = 74/150 (49.33%), Postives = 103/150 (68.67%), Query Frame = 0

Query: 49  FLQWIASFAAIYLLILDRTNWK--TNILTSLLIPYIFFSLPGLIFGFLRGEVGKWIAAIA 108
           FL+W+AS  A++L+ILD+T WK   NI+ SLL PY+F SLP +IF  LR  VGKWIA + 
Sbjct: 30  FLRWLASIVAVFLMILDQTKWKYSNNIMASLLAPYLFSSLPIVIFQVLRNGVGKWIALLT 89

Query: 109 VVLRLFFPRRFPDWLELPGALILLIVVAPGLFASTIRGDA--IGEVICLIISCYLLQEHI 168
           V+LRLF P  F + LE+PGA ILLIVV P    +  R D    G  +CL+ S YL+ +H 
Sbjct: 90  VILRLFLPNHFHESLEIPGATILLIVVTPSDIGAIFRDDLRYTGGDVCLLTSFYLINKHT 149

Query: 169 RASGGFRNSFTKANGISNTIGIVLLFVFPV 195
           +A GG +NSFT+ + ++ +I + +LFV+P+
Sbjct: 150 KACGGIKNSFTQKDKVTYSICLWILFVYPI 179

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023532118.16.4e-105100.00cold-regulated 413 plasma membrane protein 2-like [Cucurbita pepo subsp. pepo] >... [more]
XP_022948197.14.1e-10499.50cold-regulated 413 plasma membrane protein 2-like [Cucurbita moschata][more]
XP_023007529.15.4e-10499.01cold-regulated 413 plasma membrane protein 2-like [Cucurbita maxima][more]
KAG6605109.13.1e-9998.97Cold-regulated 413 plasma membrane protein 2, partial [Cucurbita argyrosperma su... [more]
XP_038902783.12.1e-9590.59cold-regulated 413 plasma membrane protein 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9SVL64.1e-7066.50Cold-regulated 413 plasma membrane protein 2 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9XIM74.6e-6669.06Cold-regulated 413 plasma membrane protein 1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
O231642.6e-6458.71Cold-regulated 413 plasma membrane protein 4 OS=Arabidopsis thaliana OX=3702 GN=... [more]
O648341.8e-3349.33Cold-regulated 413 plasma membrane protein 3 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q94AL81.3e-1228.65Cold-regulated 413 inner membrane protein 1, chloroplastic OS=Arabidopsis thalia... [more]
Match NameE-valueIdentityDescription
A0A6J1G8J02.0e-10499.50cold-regulated 413 plasma membrane protein 2-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1L5682.6e-10499.01cold-regulated 413 plasma membrane protein 2-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A1S3CAX88.4e-9589.60cold-regulated 413 plasma membrane protein 2-like OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A5A7TUZ71.1e-9489.11Cold-regulated 413 plasma membrane protein 2-like protein OS=Cucumis melo var. m... [more]
A0A6J1FVU65.5e-9488.61cold-regulated 413 plasma membrane protein 2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT3G50830.12.9e-7166.50cold-regulated 413-plasma membrane 2 [more]
AT2G15970.13.3e-6769.06cold regulated 413 plasma membrane 1 [more]
AT4G37220.11.8e-6558.71Cold acclimation protein WCOR413 family [more]
AT2G15970.22.3e-4464.71cold regulated 413 plasma membrane 1 [more]
AT2G23680.11.3e-3449.33Cold acclimation protein WCOR413 family [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008892Cold-regulated 413 proteinPFAMPF05562WCOR413coord: 9..189
e-value: 1.4E-87
score: 292.4
IPR008892Cold-regulated 413 proteinPANTHERPTHR33596COLD-REGULATED 413 PLASMA MEMBRANE PROTEIN 2coord: 1..201
NoneNo IPR availablePANTHERPTHR33596:SF18COLD-REGULATED 413 PLASMA MEMBRANE PROTEIN 1-RELATEDcoord: 1..201

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22968-RACarg22968-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane