Carg22960 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22960
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Non-symbiotic hemoglobin
Location	Carg_Chr02: 1874168 .. 1875070 (-)
RNA-Seq Expression	Carg22960
Synteny	Carg22960

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCACTTTCGGAGAATCCAAGGTTTTCACTGAACACCAAGAAGCTCTTGTGGTCAAGTCATGGAGTGTCATGAAAAAGAATGCTCCTGAATTGGCCCTCAAATTCTTCCTCAAGTAAGCTACTCCCAACCCGAACATCGTCAATTCAAAGGTTCGTATTCGTGATTTGAAACTAACCCTTTTCTTGTTTTGGTGGATTGTGTTTAGGATATTTGAAATCGCTCCGTCCGCTCAAAAGATATTTCCTTTTTTGAGAGACGCTAAAGTCCCCCTCGAACAAAACCCTAAGCTGAAACCTCATGCTTTGAGTGTCTTTACTTTGGTAAGTAAATCAGGGACGGGCCTATCACAAAAATATTCTTTTAGTGGGACGATAGCTCTGAAAAGACAGATTCTCCATCTTTTCTTTAATCTCAGAAGAAATACGATCGGGGGCTCAGATAAAATAAGAACCGCCCCTTTTCCCATTCGCAAGAACTTGATGCAAGTTTTTGTGGGATGTTTTTTTTAATGTCTTTTACATTATGAATTTGGTTTGAGTCTCGTAGACTTGTGAGTCAGCGGTTCAGCTTCGAAAAGGTGGCATAGCAGCAGCTAGAGAAACCACTATCAAGAGGCTCGGTGCCAGTCACTTCAAATATGGTGTCGTCGACGAACATTTCGATGTATCTACATTATAGTTTGATCTTATAATTTGAACTTTACCTTCATCCCGATCAAACTATGACCTAATAATGAACGTTTTTTTTGTTCATAGGTGACAAGATTTGCACTTTTGGAGACAATAAAGGAAGGAATTCCAGAGATGTGGAGTGCAGAGATGAGAGGGGCATGGGCTGACGCTTATGATCAGTTGGTGGCCGCCATTAAAGCTGAGATGAAGCCTTCTTCCACCTCTTAA

mRNA sequence

ATGGGCACTTTCGGAGAATCCAAGGTTTTCACTGAACACCAAGAAGCTCTTGTGGTCAAGTCATGGAGTGTCATGAAAAAGAATGCTCCTGAATTGGCCCTCAAATTCTTCCTCAAGATATTTGAAATCGCTCCGTCCGCTCAAAAGATATTTCCTTTTTTGAGAGACGCTAAAGTCCCCCTCGAACAAAACCCTAAGCTGAAACCTCATGCTTTGAGTGTCTTTACTTTGACTTGTGAGTCAGCGGTTCAGCTTCGAAAAGGTGGCATAGCAGCAGCTAGAGAAACCACTATCAAGAGGCTCGGTGCCAGTCACTTCAAATATGGTGTCGTCGACGAACATTTCGATGTGACAAGATTTGCACTTTTGGAGACAATAAAGGAAGGAATTCCAGAGATGTGGAGTGCAGAGATGAGAGGGGCATGGGCTGACGCTTATGATCAGTTGGTGGCCGCCATTAAAGCTGAGATGAAGCCTTCTTCCACCTCTTAA

Coding sequence (CDS)

ATGGGCACTTTCGGAGAATCCAAGGTTTTCACTGAACACCAAGAAGCTCTTGTGGTCAAGTCATGGAGTGTCATGAAAAAGAATGCTCCTGAATTGGCCCTCAAATTCTTCCTCAAGATATTTGAAATCGCTCCGTCCGCTCAAAAGATATTTCCTTTTTTGAGAGACGCTAAAGTCCCCCTCGAACAAAACCCTAAGCTGAAACCTCATGCTTTGAGTGTCTTTACTTTGACTTGTGAGTCAGCGGTTCAGCTTCGAAAAGGTGGCATAGCAGCAGCTAGAGAAACCACTATCAAGAGGCTCGGTGCCAGTCACTTCAAATATGGTGTCGTCGACGAACATTTCGATGTGACAAGATTTGCACTTTTGGAGACAATAAAGGAAGGAATTCCAGAGATGTGGAGTGCAGAGATGAGAGGGGCATGGGCTGACGCTTATGATCAGTTGGTGGCCGCCATTAAAGCTGAGATGAAGCCTTCTTCCACCTCTTAA

Protein sequence

MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVPLEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRFALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS

Homology

[Close]

BLAST of Carg22960 vs. NCBI nr
Match: KAG7035102.1 (Non-symbiotic hemoglobin 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 323.6 bits (828), Expect = 1.0e-84
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP
Sbjct: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF
Sbjct: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 164
           ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS
Sbjct: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 163

[Close]

BLAST of Carg22960 vs. NCBI nr
Match: KAG6605102.1 (Non-symbiotic hemoglobin 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 323.6 bits (828), Expect = 1.0e-84
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP
Sbjct: 11  MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 70

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF
Sbjct: 71  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 130

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 164
           ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS
Sbjct: 131 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 173

[Close]

BLAST of Carg22960 vs. NCBI nr
Match: XP_022947860.1 (plastid division protein PDV2-like [Cucurbita moschata])

HSP 1 Score: 322.4 bits (825), Expect = 2.3e-84
Identity = 162/163 (99.39%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           MGTFGESKVFTEHQEALV+KSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP
Sbjct: 358 MGTFGESKVFTEHQEALVLKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 417

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF
Sbjct: 418 LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 477

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 164
           ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS
Sbjct: 478 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 520

[Close]

BLAST of Carg22960 vs. NCBI nr
Match: KAG6605095.1 (Non-symbiotic hemoglobin 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 322.4 bits (825), Expect = 2.3e-84
Identity = 162/163 (99.39%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           MGTFGESKVFTEHQEALV+KSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP
Sbjct: 11  MGTFGESKVFTEHQEALVLKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 70

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF
Sbjct: 71  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 130

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 164
           ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS
Sbjct: 131 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 173

[Close]

BLAST of Carg22960 vs. NCBI nr
Match: XP_023533133.1 (plastid division protein PDV2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 321.2 bits (822), Expect = 5.0e-84
Identity = 162/163 (99.39%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           M TFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP
Sbjct: 323 MATFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 382

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF
Sbjct: 383 LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 442

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 164
           ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS
Sbjct: 443 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 485

[Close]

BLAST of Carg22960 vs. ExPASy Swiss-Prot
Match: Q9FVL0 (Non-symbiotic hemoglobin 1 OS=Medicago sativa OX=3879 GN=MHB1 PE=2 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 1.7e-63
Identity = 119/161 (73.91%), Postives = 139/161 (86.34%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           MGT  ++K FTE QEALVVKSW+ MKKN+ EL LK FLKIFEIAPSAQK+F FL+D+KVP
Sbjct: 1   MGTL-DTKGFTEEQEALVVKSWNAMKKNSAELGLKLFLKIFEIAPSAQKLFSFLKDSKVP 60

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQN KLKPHA+SVF +TCESAVQLRK G    RE+++K+LGA+HFKYGVVDEHF+VT+F
Sbjct: 61  LEQNTKLKPHAMSVFLMTCESAVQLRKSGKVTVRESSLKKLGANHFKYGVVDEHFEVTKF 120

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSS 162
           ALLETIKE +PEMWS  M+ AW +AYDQLV AIK+EMKPSS
Sbjct: 121 ALLETIKEAVPEMWSPAMKNAWGEAYDQLVNAIKSEMKPSS 160

[Close]

BLAST of Carg22960 vs. ExPASy Swiss-Prot
Match: Q947C5 (Non-symbiotic hemoglobin 1 OS=Gossypium hirsutum OX=3635 GN=HB1 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 1.5e-62
Identity = 118/161 (73.29%), Postives = 136/161 (84.47%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           M T+ E KVFTE QEALVVKSW+VMKK   EL LKFFLKIFEIAPSA+K+F FLRD+ VP
Sbjct: 1   MATY-EGKVFTEEQEALVVKSWTVMKKKTAELGLKFFLKIFEIAPSAKKLFSFLRDSNVP 60

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQN KLKPHA+SVF +TCESAVQLRK G    RE+ +K+LGA+HFKYGVVDEHF+VT+F
Sbjct: 61  LEQNTKLKPHAMSVFVMTCESAVQLRKAGKVTVRESNLKKLGATHFKYGVVDEHFEVTKF 120

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSS 162
           ALLETIKE +P+MWS EM+ AW +AYD+LVAAIK EMK  S
Sbjct: 121 ALLETIKEAVPDMWSDEMKNAWGEAYDRLVAAIKIEMKACS 160

[Close]

BLAST of Carg22960 vs. ExPASy Swiss-Prot
Match: P07803 (Non-symbiotic hemoglobin OS=Trema tomentosa OX=3480 GN=GLB1 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 7.3e-62
Identity = 116/155 (74.84%), Postives = 135/155 (87.10%), Query Frame = 0


Query: 8   KVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVPLEQNPKL 67
           KVFTE QEALVVKSW+VMKKN+ EL LKFFLKIFEIAPSA+ +F +L+D+ +PLEQNPKL
Sbjct: 8   KVFTEEQEALVVKSWAVMKKNSAELGLKFFLKIFEIAPSAKNLFSYLKDSPIPLEQNPKL 67

Query: 68  KPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRFALLETIK 127
           KPHA++VF +TCESAVQLRK G    RE+ +KRLGA HFK GVV+EHF+ TRFALLETIK
Sbjct: 68  KPHAMTVFVMTCESAVQLRKAGKVTVRESNLKRLGAIHFKNGVVNEHFE-TRFALLETIK 127

Query: 128 EGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSST 163
           E +PEMWS EM+ AW +AYDQLVAAIK+EMKPSST
Sbjct: 128 EAVPEMWSPEMKNAWGEAYDQLVAAIKSEMKPSST 161

[Close]

BLAST of Carg22960 vs. ExPASy Swiss-Prot
Match: P23244 (Hemoglobin-2 OS=Casuarina glauca OX=3522 GN=HBII PE=3 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 9.6e-62
Identity = 115/156 (73.72%), Postives = 133/156 (85.26%), Query Frame = 0


Query: 6   ESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVPLEQNP 65
           E + FTE QEALVVKSWS MK NA EL LKFFLKIFEIAPSAQK+F FL+D+ VPLE+NP
Sbjct: 5   EGRGFTEEQEALVVKSWSAMKPNAGELGLKFFLKIFEIAPSAQKLFSFLKDSNVPLERNP 64

Query: 66  KLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRFALLET 125
           KLK HA+SVF +TCESAVQLRK G    RE+++K+LGASHFK+GV DEHF+VT+FALLET
Sbjct: 65  KLKSHAMSVFLMTCESAVQLRKAGKVTVRESSLKKLGASHFKHGVADEHFEVTKFALLET 124

Query: 126 IKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSS 162
           IKE +PE WS EM+ AW +AYD+LVAAIK EMKPSS
Sbjct: 125 IKEAVPETWSPEMKNAWGEAYDKLVAAIKLEMKPSS 160

[Close]

BLAST of Carg22960 vs. ExPASy Swiss-Prot
Match: P68168 (Non-legume hemoglobin OS=Parasponia andersonii OX=3476 PE=1 SV=2)

HSP 1 Score: 237.3 bits (604), Expect = 1.2e-61
Identity = 115/156 (73.72%), Postives = 135/156 (86.54%), Query Frame = 0


Query: 7   SKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVPLEQNPK 66
           +KVFTE QEALVVK+W+VMKKN+ EL L+FFLKIFEIAPSA+ +F +L+D+ VPLEQNPK
Sbjct: 7   NKVFTEEQEALVVKAWAVMKKNSAELGLQFFLKIFEIAPSAKNLFSYLKDSPVPLEQNPK 66

Query: 67  LKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRFALLETI 126
           LKPHA +VF +TCESAVQLRK G A  +E+ +KR+GA HFK GVV+EHF+VTRFALLETI
Sbjct: 67  LKPHATTVFVMTCESAVQLRKAGKATVKESDLKRIGAIHFKTGVVNEHFEVTRFALLETI 126

Query: 127 KEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSST 163
           KE +PEMWS EM+ AW  AYDQLVAAIK EMKPSST
Sbjct: 127 KEAVPEMWSPEMKNAWGVAYDQLVAAIKFEMKPSST 162

[Close]

BLAST of Carg22960 vs. ExPASy TrEMBL
Match: A0A6J1G827 (plastid division protein PDV2-like OS=Cucurbita moschata OX=3662 GN=LOC111451618 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.1e-84
Identity = 162/163 (99.39%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           MGTFGESKVFTEHQEALV+KSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP
Sbjct: 358 MGTFGESKVFTEHQEALVLKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 417

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF
Sbjct: 418 LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 477

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 164
           ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS
Sbjct: 478 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 520

[Close]

BLAST of Carg22960 vs. ExPASy TrEMBL
Match: A0A6J1KWX0 (plastid division protein PDV2-like OS=Cucurbita maxima OX=3661 GN=LOC111499505 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.3e-82
Identity = 157/163 (96.32%), Postives = 161/163 (98.77%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           MG+FGESKVFTEHQEALV+KSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP
Sbjct: 342 MGSFGESKVFTEHQEALVLKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 401

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQNPKLKPHAL+VFTL CESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF
Sbjct: 402 LEQNPKLKPHALNVFTLACESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 461

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSSTS 164
           ALLETIKEGIPEMWS EMRGAWA+AYDQLVAAIKAEMKPSSTS
Sbjct: 462 ALLETIKEGIPEMWSVEMRGAWAEAYDQLVAAIKAEMKPSSTS 504

[Close]

BLAST of Carg22960 vs. ExPASy TrEMBL
Match: A0A5D3CTW6 (Plastid division protein PDV2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001460 PE=3 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.5e-73
Identity = 138/159 (86.79%), Postives = 152/159 (95.60%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           MG+ GE KVFTE QEALV+KSWSVMKKNA +LA KFFLKIFEIAPSAQK+FPFLRD+KVP
Sbjct: 1   MGSCGEGKVFTEEQEALVIKSWSVMKKNAADLAFKFFLKIFEIAPSAQKMFPFLRDSKVP 60

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQNPKLKPHAL+VFTLTCESAVQLRKGGIAAA+ETTIKRLGA+H KYGV+DEHF+VT+F
Sbjct: 61  LEQNPKLKPHALNVFTLTCESAVQLRKGGIAAAKETTIKRLGATHLKYGVLDEHFEVTKF 120

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKP 160
           ALLETIKEGIPEMWS EM+GAWA+AYDQLV+AIKAEMKP
Sbjct: 121 ALLETIKEGIPEMWSVEMKGAWAEAYDQLVSAIKAEMKP 159

[Close]

BLAST of Carg22960 vs. ExPASy TrEMBL
Match: A0A1S4E332 (plastid division protein PDV2 OS=Cucumis melo OX=3656 GN=LOC103498462 PE=3 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.5e-73
Identity = 138/159 (86.79%), Postives = 152/159 (95.60%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           MG+ GE KVFTE QEALV+KSWSVMKKNA +LA KFFLKIFEIAPSAQK+FPFLRD+KVP
Sbjct: 316 MGSCGEGKVFTEEQEALVIKSWSVMKKNAADLAFKFFLKIFEIAPSAQKMFPFLRDSKVP 375

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQNPKLKPHAL+VFTLTCESAVQLRKGGIAAA+ETTIKRLGA+H KYGV+DEHF+VT+F
Sbjct: 376 LEQNPKLKPHALNVFTLTCESAVQLRKGGIAAAKETTIKRLGATHLKYGVLDEHFEVTKF 435

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKP 160
           ALLETIKEGIPEMWS EM+GAWA+AYDQLV+AIKAEMKP
Sbjct: 436 ALLETIKEGIPEMWSVEMKGAWAEAYDQLVSAIKAEMKP 474

[Close]

BLAST of Carg22960 vs. ExPASy TrEMBL
Match: A0A0A0LLW6 (GLOBIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G238880 PE=3 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 2.1e-72
Identity = 134/159 (84.28%), Postives = 153/159 (96.23%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           MG+ GE K+F+E QEALV+KSWSVMKKNAP+LA KFFLKIFEIAPSAQK+FPFLRD+KVP
Sbjct: 1   MGSCGERKIFSEEQEALVIKSWSVMKKNAPDLAFKFFLKIFEIAPSAQKMFPFLRDSKVP 60

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
           LEQNPKLKPHAL+VFTLTCESAVQLRKGGIAAA+E+T+KRLGA+H KYGV+DEHF+VT+F
Sbjct: 61  LEQNPKLKPHALNVFTLTCESAVQLRKGGIAAAKESTMKRLGATHLKYGVLDEHFEVTKF 120

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMKP 160
           ALLETIKEGIPEMWS EM+GAWA+AYDQLV+AIKA+MKP
Sbjct: 121 ALLETIKEGIPEMWSVEMKGAWAEAYDQLVSAIKAQMKP 159

[Close]

BLAST of Carg22960 vs. TAIR 10
Match: AT2G16060.1 (hemoglobin 1 )

HSP 1 Score: 229.9 bits (585), Expect = 1.4e-60
Identity = 112/153 (73.20%), Postives = 128/153 (83.66%), Query Frame = 0


Query: 9   VFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVPLEQNPKLK 68
           VFTE QEALVVKSWSVMKKN+ EL LK F+KIFEIAP+ +K+F FLRD+ +P EQNPKLK
Sbjct: 8   VFTEEQEALVVKSWSVMKKNSAELGLKLFIKIFEIAPTTKKMFSFLRDSPIPAEQNPKLK 67

Query: 69  PHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRFALLETIKE 128
           PHA+SVF + CESAVQLRK G    RETT+KRLGASH KYGVVDEHF+V ++ALLETIKE
Sbjct: 68  PHAMSVFVMCCESAVQLRKTGKVTVRETTLKRLGASHSKYGVVDEHFEVAKYALLETIKE 127

Query: 129 GIPEMWSAEMRGAWADAYDQLVAAIKAEMKPSS 162
            +PEMWS EM+ AW  AYD LVAAIKAEM  S+
Sbjct: 128 AVPEMWSPEMKVAWGQAYDHLVAAIKAEMNLSN 160

[Close]

BLAST of Carg22960 vs. TAIR 10
Match: AT3G10520.1 (haemoglobin 2 )

HSP 1 Score: 163.3 bits (412), Expect = 1.6e-40
Identity = 80/158 (50.63%), Postives = 110/158 (69.62%), Query Frame = 0


Query: 1   MGTFGESKVFTEHQEALVVKSWSVMKKNAPELALKFFLKIFEIAPSAQKIFPFLRDAKVP 60
           MG  G    FTE QEALV +SW ++K++ P+ +L FF +I EIAP+A+ +F FLRD+   
Sbjct: 1   MGEIG----FTEKQEALVKESWEILKQDIPKYSLHFFSQILEIAPAAKGLFSFLRDSDEV 60

Query: 61  LEQNPKLKPHALSVFTLTCESAVQLRKGGIAAARETTIKRLGASHFKYGVVDEHFDVTRF 120
              NPKLK HA+ VF +TCE+A+QLR+ G     +TT++ LG+ H K GV+D HF+V + 
Sbjct: 61  PHNNPKLKAHAVKVFKMTCETAIQLREEGKVVVADTTLQYLGSIHLKSGVIDPHFEVVKE 120

Query: 121 ALLETIKEGIPEMWSAEMRGAWADAYDQLVAAIKAEMK 159
           ALL T+KEG+ E ++ E+ GAW+ AYD L  AIK EMK
Sbjct: 121 ALLRTLKEGLGEKYNEEVEGAWSQAYDHLALAIKTEMK 154

The following BLAST results are available for this feature:

BLAST of Carg22960 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035102.1	1.0e-84	100.00	Non-symbiotic hemoglobin 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6605102.1	1.0e-84	100.00	Non-symbiotic hemoglobin 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022947860.1	2.3e-84	99.39	plastid division protein PDV2-like [Cucurbita moschata]	[more]
KAG6605095.1	2.3e-84	99.39	Non-symbiotic hemoglobin 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023533133.1	5.0e-84	99.39	plastid division protein PDV2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg22960 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FVL0	1.7e-63	73.91	Non-symbiotic hemoglobin 1 OS=Medicago sativa OX=3879 GN=MHB1 PE=2 SV=1	[more]
Q947C5	1.5e-62	73.29	Non-symbiotic hemoglobin 1 OS=Gossypium hirsutum OX=3635 GN=HB1 PE=2 SV=1	[more]
P07803	7.3e-62	74.84	Non-symbiotic hemoglobin OS=Trema tomentosa OX=3480 GN=GLB1 PE=1 SV=1	[more]
P23244	9.6e-62	73.72	Hemoglobin-2 OS=Casuarina glauca OX=3522 GN=HBII PE=3 SV=1	[more]
P68168	1.2e-61	73.72	Non-legume hemoglobin OS=Parasponia andersonii OX=3476 PE=1 SV=2	[more]

BLAST of Carg22960 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G827	1.1e-84	99.39	plastid division protein PDV2-like OS=Cucurbita moschata OX=3662 GN=LOC111451618...	[more]
A0A6J1KWX0	2.3e-82	96.32	plastid division protein PDV2-like OS=Cucurbita maxima OX=3661 GN=LOC111499505 P...	[more]
A0A5D3CTW6	2.5e-73	86.79	Plastid division protein PDV2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S4E332	2.5e-73	86.79	plastid division protein PDV2 OS=Cucumis melo OX=3656 GN=LOC103498462 PE=3 SV=1	[more]
A0A0A0LLW6	2.1e-72	84.28	GLOBIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G238880 PE=3...	[more]

BLAST of Carg22960 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G16060.1	1.4e-60	73.20	hemoglobin 1	[more]
AT3G10520.1	1.6e-40	50.63	haemoglobin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00188	PLANTGLOBIN	coord: 129..155 score: 54.64 coord: 11..28 score: 63.31 coord: 36..56 score: 60.68 coord: 64..92 score: 57.26
None	No IPR available	PANTHER	PTHR22924:SF94	HEMOGLOBIN 1, GLB1,NSHB1,ATGLB1,HB1	coord: 8..162
None	No IPR available	CDD	cd14784	class1_nsHb-like	coord: 10..158 e-value: 4.82893E-89 score: 254.748
IPR000971	Globin	PFAM	PF00042	Globin	coord: 14..122 e-value: 3.0E-18 score: 66.4
IPR000971	Globin	PROSITE	PS01033	GLOBIN	coord: 10..158 score: 27.301653
IPR012292	Globin/Protoglobin	GENE3D	1.10.490.10	Globins	coord: 9..156 e-value: 3.5E-54 score: 185.0
IPR001032	Leghaemoglobin	PANTHER	PTHR22924	LEGHEMOGLOBIN-RELATED	coord: 8..162
IPR019824	Leghaemoglobin, iron-binding site	PROSITE	PS00208	PLANT_GLOBIN	coord: 64..75
IPR009050	Globin-like superfamily	SUPERFAMILY	46458	Globin-like	coord: 8..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg22960-RA	Carg22960-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010020	chloroplast fission
biological_process	GO:0015671	oxygen transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0019825	oxygen binding
molecular_function	GO:0005344	oxygen carrier activity