Carg22943 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22943
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAB hydrolase-1 domain-containing protein
LocationCarg_Chr02: 1753542 .. 1754368 (+)
RNA-Seq ExpressionCarg22943
SyntenyCarg22943
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAATTAGAGAATCCATGGCGAACATTTTCCACGTCTACCACCGGGTTCTCCGGTGGCTAATGAAGGTCGCCGGAGTCCGGCAAGAACTGGTCCAGATAGAACCAGGAACCACGATGAATTTCTGGGTACCCACCAGAAACCCGAAAAAAAACCCGAATCCACCGGTGGTCCTTCTCCATGGATTCGCCGGCAACGGGATCATGACGTGGCAATTTCAGGTGATGAATTTGGCCAGAAACTACGCCGTCTATGTACCGGACTTTCTCTTCTTCGGTGAGTCGGTGACGGACCGGGCGGAGAGATCGCCGGAGTTTCAGGCGGAGTGCGTGGTGAAGGGGCTGAGGAGGCTGGGGGTTGAGAGGCCGTGCGTTTTGGTGGGGTTTAGCTATGGGGCAATGGTGGGGTTTAGGCTGGCTGAGATGTACCCGGATTTGGTGGCGGCGATGGTGGTCACGTCGGCGCCGACGGCGTTGACGGAGTCGCTCAGCCGTGTGGCGCTGCAGGAGATTGGGTATAAAAGTTGGTCGGATTTCTTGATTCCGAACACGGTCAACGGTGTGAAATCCTTGTTTGAAATTGGTTCCTTTGACTTCCCGGCGCTTCCTAATTGGATTATGAAACATTATTTGGAGGTATTTTTAATTTTAAATTTTTTTAATAAAATTAAAATGTATTATTAATTTTTTTTTAAAGTTTAGAGATATTTTTTAAACAAATTATAACATCGTCACAGGCAATGGTTAAGTACAGAAACGAACAAGCAGAACTTCTTCGAGCATTGGTGGTACCGGATGATTTTACCATCTCTCAATTCCCACAGGTTTAA

mRNA sequence

AGAATTAGAGAATCCATGGCGAACATTTTCCACGTCTACCACCGGGTTCTCCGGTGGCTAATGAAGGTCGCCGGAGTCCGGCAAGAACTGGTCCAGATAGAACCAGGAACCACGATGAATTTCTGGGTACCCACCAGAAACCCGAAAAAAAACCCGAATCCACCGGTGGTCCTTCTCCATGGATTCGCCGGCAACGGGATCATGACGTGGCAATTTCAGGTGATGAATTTGGCCAGAAACTACGCCGTCTATGTACCGGACTTTCTCTTCTTCGGTGAGTCGGTGACGGACCGGGCGGAGAGATCGCCGGAGTTTCAGGCGGAGTGCGTGGTGAAGGGGCTGAGGAGGCTGGGGGTTGAGAGGCCGTGCGTTTTGGTGGGGTTTAGCTATGGGGCAATGGTGGGGTTTAGGCTGGCTGAGATGTACCCGGATTTGGTGGCGGCGATGGTGGTCACGTCGGCGCCGACGGCGTTGACGGAGTCGCTCAGCCGTGTGGCGCTGCAGGAGATTGGGTATAAAAGTTGGTCGGATTTCTTGATTCCGAACACGGTCAACGGTGTGAAATCCTTGTTTGAAATTGGTTCCTTTGACTTCCCGGCGCTTCCTAATTGGATTATGAAACATTATTTGGAGGCAATGGTTAAGTACAGAAACGAACAAGCAGAACTTCTTCGAGCATTGGTGGTACCGGATGATTTTACCATCTCTCAATTCCCACAGGTTTAA

Coding sequence (CDS)

AGAATTAGAGAATCCATGGCGAACATTTTCCACGTCTACCACCGGGTTCTCCGGTGGCTAATGAAGGTCGCCGGAGTCCGGCAAGAACTGGTCCAGATAGAACCAGGAACCACGATGAATTTCTGGGTACCCACCAGAAACCCGAAAAAAAACCCGAATCCACCGGTGGTCCTTCTCCATGGATTCGCCGGCAACGGGATCATGACGTGGCAATTTCAGGTGATGAATTTGGCCAGAAACTACGCCGTCTATGTACCGGACTTTCTCTTCTTCGGTGAGTCGGTGACGGACCGGGCGGAGAGATCGCCGGAGTTTCAGGCGGAGTGCGTGGTGAAGGGGCTGAGGAGGCTGGGGGTTGAGAGGCCGTGCGTTTTGGTGGGGTTTAGCTATGGGGCAATGGTGGGGTTTAGGCTGGCTGAGATGTACCCGGATTTGGTGGCGGCGATGGTGGTCACGTCGGCGCCGACGGCGTTGACGGAGTCGCTCAGCCGTGTGGCGCTGCAGGAGATTGGGTATAAAAGTTGGTCGGATTTCTTGATTCCGAACACGGTCAACGGTGTGAAATCCTTGTTTGAAATTGGTTCCTTTGACTTCCCGGCGCTTCCTAATTGGATTATGAAACATTATTTGGAGGCAATGGTTAAGTACAGAAACGAACAAGCAGAACTTCTTCGAGCATTGGTGGTACCGGATGATTTTACCATCTCTCAATTCCCACAGGTTTAA

Protein sequence

RIRESMANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPNTVNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQV
Homology
BLAST of Carg22943 vs. NCBI nr
Match: KAG7035085.1 (Epoxide hydrolase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 494.2 bits (1271), Expect = 6.4e-136
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0

Query: 1   RIRESMANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLH 60
           RIRESMANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLH
Sbjct: 1   RIRESMANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLH 60

Query: 61  GFAGNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVE 120
           GFAGNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVE
Sbjct: 61  GFAGNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVE 120

Query: 121 RPCVLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLI 180
           RPCVLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLI
Sbjct: 121 RPCVLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLI 180

Query: 181 PNTVNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQ 240
           PNTVNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQ
Sbjct: 181 PNTVNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQ 240

Query: 241 V 242
           V
Sbjct: 241 V 241

BLAST of Carg22943 vs. NCBI nr
Match: KAG6605077.1 (Epoxide hydrolase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 479.6 bits (1233), Expect = 1.6e-131
Identity = 233/235 (99.15%), Postives = 234/235 (99.57%), Query Frame = 0

Query: 6   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 65
           MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNP KNPNPPVVLLHGFAGN
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPIKNPNPPVVLLHGFAGN 60

Query: 66  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 125
           GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 120

Query: 126 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPNTVN 185
           VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSD+LIPNTVN
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDYLIPNTVN 180

Query: 186 GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQ 241
           GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQ
Sbjct: 181 GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQ 235

BLAST of Carg22943 vs. NCBI nr
Match: XP_023533135.1 (epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 479.6 bits (1233), Expect = 1.6e-131
Identity = 232/236 (98.31%), Postives = 235/236 (99.58%), Query Frame = 0

Query: 6   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 65
           MANIFHVYHRVLRWLMKVAGVRQELVQI+PGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIQPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 60

Query: 66  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 125
           GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLR+LGVERPCVL
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRKLGVERPCVL 120

Query: 126 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPNTVN 185
           VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSD+LIPNTVN
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDYLIPNTVN 180

Query: 186 GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQV 242
           GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYR EQAELLRALVVPDDFTISQFPQV
Sbjct: 181 GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRKEQAELLRALVVPDDFTISQFPQV 236

BLAST of Carg22943 vs. NCBI nr
Match: XP_022947943.1 (epoxide hydrolase 4-like [Cucurbita moschata])

HSP 1 Score: 468.4 bits (1204), Expect = 3.8e-128
Identity = 229/235 (97.45%), Postives = 231/235 (98.30%), Query Frame = 0

Query: 6   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 65
           MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKN NPPVVLLHGFAGN
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNRNPPVVLLHGFAGN 60

Query: 66  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 125
           GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAE VVKGLR+LGVERPCVL
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAEGVVKGLRKLGVERPCVL 120

Query: 126 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPNTVN 185
           VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVAL EIGYKSWSD+LIPNTVN
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALLEIGYKSWSDYLIPNTVN 180

Query: 186 GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQ 241
           GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYR EQAELLRALVVPDDFTISQFPQ
Sbjct: 181 GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRKEQAELLRALVVPDDFTISQFPQ 235

BLAST of Carg22943 vs. NCBI nr
Match: XP_023006837.1 (epoxide hydrolase 4-like [Cucurbita maxima])

HSP 1 Score: 411.4 bits (1056), Expect = 5.4e-111
Identity = 197/206 (95.63%), Postives = 202/206 (98.06%), Query Frame = 0

Query: 6   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 65
           MANIFHVYHRVLRWLMKVAGVRQELVQI+PGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIQPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 60

Query: 66  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 125
           GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 120

Query: 126 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPNTVN 185
           VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPT LTESLSR ALQEIGYKSWSD+LIPNTV 
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTVLTESLSREALQEIGYKSWSDYLIPNTVK 180

Query: 186 GVKSLFEIGSFDFPALPNWIMKHYLE 212
           GVKSLFEIGSFDFPALPNWIM+ Y++
Sbjct: 181 GVKSLFEIGSFDFPALPNWIMEQYVK 206

BLAST of Carg22943 vs. ExPASy Swiss-Prot
Match: Q8IUS5 (Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2)

HSP 1 Score: 55.1 bits (131), Expect = 1.3e-06
Identity = 37/134 (27.61%), Postives = 65/134 (48.51%), Query Frame = 0

Query: 54  PPVVLLHGFAGNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKG 113
           P ++LLHGF      +W++Q+      Y V   D   +GE  TD       ++ +C++  
Sbjct: 94  PLMLLLHGFP-EFWYSWRYQLREFKSEYRVVALDLRGYGE--TDAPIHRQNYKLDCLITD 153

Query: 114 LR----RLGVERPCVLVGFSYGAMVGFRLAEMYPDLVAAMVVTS--APTALTESLSRVAL 173
           ++     LG  + CVL+G  +G M+ + +A  YP++V  ++V +   P   TE + R   
Sbjct: 154 IKDILDSLGYSK-CVLIGHDWGGMIAWLIAICYPEMVMKLIVINFPHPNVFTEYILRHPA 213

Query: 174 QEIGYKSWSDFLIP 182
           Q +    +  F IP
Sbjct: 214 QLLKSSYYYFFQIP 223

BLAST of Carg22943 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 55.1 bits (131), Expect = 1.3e-06
Identity = 39/149 (26.17%), Postives = 70/149 (46.98%), Query Frame = 0

Query: 54  PPVVLLHGFAGNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKG 113
           P ++LLHGF      +W+ Q+      Y V   D   +GES  D       ++ +C++  
Sbjct: 92  PLMLLLHGFP-EFWYSWRHQLREFKSEYRVVALDLRGYGES--DAPAHQESYKLDCLIAD 151

Query: 114 LR----RLGVERPCVLVGFSYGAMVGFRLAEMYPDLVAAMVVTS--APTALTESLSRVAL 173
           ++     LG  + CVL+G  +G M+ + +A  YP+++  ++V +   P+  TE + R   
Sbjct: 152 IKDILDSLGYSK-CVLIGHDWGGMIAWLIAVCYPEMIMKLIVINFPHPSVFTEYILRHPA 211

Query: 174 QEIGYKSWSDFLIPN------TVNGVKSL 191
           Q      +  F IP       ++N  K+L
Sbjct: 212 QLFRSSFYYFFQIPRFPEFMFSINDFKAL 236

BLAST of Carg22943 vs. ExPASy TrEMBL
Match: A0A6J1G806 (epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451675 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 1.8e-128
Identity = 229/235 (97.45%), Postives = 231/235 (98.30%), Query Frame = 0

Query: 6   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 65
           MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKN NPPVVLLHGFAGN
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNRNPPVVLLHGFAGN 60

Query: 66  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 125
           GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAE VVKGLR+LGVERPCVL
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAEGVVKGLRKLGVERPCVL 120

Query: 126 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPNTVN 185
           VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVAL EIGYKSWSD+LIPNTVN
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALLEIGYKSWSDYLIPNTVN 180

Query: 186 GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQ 241
           GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYR EQAELLRALVVPDDFTISQFPQ
Sbjct: 181 GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRKEQAELLRALVVPDDFTISQFPQ 235

BLAST of Carg22943 vs. ExPASy TrEMBL
Match: A0A6J1KWX2 (epoxide hydrolase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111499509 PE=4 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.6e-111
Identity = 197/206 (95.63%), Postives = 202/206 (98.06%), Query Frame = 0

Query: 6   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 65
           MANIFHVYHRVLRWLMKVAGVRQELVQI+PGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIQPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 60

Query: 66  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 125
           GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 120

Query: 126 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPNTVN 185
           VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPT LTESLSR ALQEIGYKSWSD+LIPNTV 
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTVLTESLSREALQEIGYKSWSDYLIPNTVK 180

Query: 186 GVKSLFEIGSFDFPALPNWIMKHYLE 212
           GVKSLFEIGSFDFPALPNWIM+ Y++
Sbjct: 181 GVKSLFEIGSFDFPALPNWIMEQYVK 206

BLAST of Carg22943 vs. ExPASy TrEMBL
Match: A0A5A7TQB4 (Epoxide hydrolase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00590 PE=4 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 1.2e-90
Identity = 160/236 (67.80%), Postives = 192/236 (81.36%), Query Frame = 0

Query: 8   NIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKN--PNPPVVLLHGFAGN 67
           NIFH+Y  VLRW+MK AG+    +QIEPGT+MNFWVP R P KN   +PP++ LHGFA N
Sbjct: 4   NIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFAAN 63

Query: 68  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 127
            IMTWQFQV+  A+ +AVYVPDF+FFG+SVTDR +RSPEFQAECV +GLR+LGVER  VL
Sbjct: 64  AIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRFVL 123

Query: 128 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPNTVN 187
           VGFSYGAMVGFRLAE YP+LV AMVVT+APT LTE ++  AL++IGYKSWS++LIP TV 
Sbjct: 124 VGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPETVK 183

Query: 188 GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQV 242
           GVKS+ EI SF+FP  P WI KHYLE M+ +  E+AELL ALV P+D TIS++PQV
Sbjct: 184 GVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQV 239

BLAST of Carg22943 vs. ExPASy TrEMBL
Match: A0A1S4E310 (uncharacterized protein LOC103499739 OS=Cucumis melo OX=3656 GN=LOC103499739 PE=4 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 3.4e-90
Identity = 159/235 (67.66%), Postives = 191/235 (81.28%), Query Frame = 0

Query: 8   NIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKN--PNPPVVLLHGFAGN 67
           NIFH+Y  VLRW+MK AG+    +QIEPGT+MNFWVP R P KN   +PP++ LHGFA N
Sbjct: 4   NIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFAAN 63

Query: 68  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 127
            IMTWQFQV+  A+ +AVYVPDF+FFG+SVTDR +RSPEFQAECV +GLR+LGVER  VL
Sbjct: 64  AIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRFVL 123

Query: 128 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPNTVN 187
           VGFSYGAMVGFRLAE YP+LV AMVVT+APT LTE ++  AL++IGYKSWS++LIP TV 
Sbjct: 124 VGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPETVK 183

Query: 188 GVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQ 241
           GVKS+ EI SF+FP  P WI KHYLE M+ +  E+AELL ALV P+D TIS++PQ
Sbjct: 184 GVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQ 238

BLAST of Carg22943 vs. ExPASy TrEMBL
Match: A0A0A0LLY6 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247580 PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.8e-88
Identity = 156/237 (65.82%), Postives = 193/237 (81.43%), Query Frame = 0

Query: 6   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKN--PNPPVVLLHGFA 65
           M NIFH+Y +VLRW+MK AG++   +QI+PGT MNFWVP   P KN   +PP++ LHGFA
Sbjct: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60

Query: 66  GNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPC 125
            N IMTWQFQV+  A+N+AVYVPDF+FFG+SVTDR +R+ EFQAECVV+GLR+LGV+R  
Sbjct: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRTTEFQAECVVEGLRKLGVDRRF 120

Query: 126 VLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPNT 185
           VLVGFSYGAMVGFRLAEMYP++V AMVVT+APT LTE ++  A+++IGYKSWS++LIP T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180

Query: 186 VNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPDDFTISQFPQ 241
           V G  S+ +I SF+FP  P WI K YLEAMV +R E+AELL ALV P+D TISQ+PQ
Sbjct: 181 VKGAISMLQIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQ 237

BLAST of Carg22943 vs. TAIR 10
Match: AT4G36610.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 222.2 bits (565), Expect = 4.4e-58
Identity = 119/248 (47.98%), Postives = 161/248 (64.92%), Query Frame = 0

Query: 6   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKN-----------PNP 65
           M N   V   +L  LMK+AGV    ++IEPGT +NFWVP    KKN           P  
Sbjct: 1   MVNFVEVQKPLLYGLMKMAGVVPYTLEIEPGTKINFWVPKETLKKNSGTGKPTKPDKPKK 60

Query: 66  PVVLL-HGFAGNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKG 125
           PVVLL HGFAG GI+TWQFQV  L++ Y+VY+PD LFFG S TD ++RSP FQA+C+VKG
Sbjct: 61  PVVLLIHGFAGEGIVTWQFQVGALSKKYSVYIPDLLFFGGSYTDNSDRSPAFQADCLVKG 120

Query: 126 LRRLGVERPCVLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYK 185
           LR LGV++  V VGFSYG MV F++AE YPD+V A+VV+ +   +T++++  +L  +G+ 
Sbjct: 121 LRILGVDK-FVPVGFSYGGMVAFKIAEAYPDMVRAIVVSGSIPTMTDTINEASLNRLGFS 180

Query: 186 SWSDFLIPNTVNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPD-D 241
           S +D L+P +V G+K+LF I        P  + K Y+E M   R E+AELL A+VV + +
Sbjct: 181 SSTDLLLPTSVTGLKALFTIAVHKPLWFPKRLFKDYIEVMFNNRKERAELLEAVVVSNKE 240

BLAST of Carg22943 vs. TAIR 10
Match: AT2G18360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 7.4e-58
Identity = 117/250 (46.80%), Postives = 162/250 (64.80%), Query Frame = 0

Query: 6   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKK-------------NP 65
           M N   +   +L  LMK+AGV    V++EPGT MNFW+P    KK              P
Sbjct: 1   MVNFVDLQKPLLYRLMKLAGVIPYTVELEPGTKMNFWIPKETLKKTKKSDKNFAVEPQKP 60

Query: 66  NPPVVL-LHGFAGNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVV 125
             PV+L +HGFA  GI+TWQFQV +LA+ Y+VY+PD LFFG S +D A+RSP FQA C+V
Sbjct: 61  TKPVLLFIHGFAAEGIVTWQFQVGSLAKKYSVYIPDLLFFGGSYSDNADRSPAFQAHCLV 120

Query: 126 KGLRRLGVERPCVLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIG 185
           K LR LG+E+   LVGFSYG MV F++AE YP++V AMVV+ +  A+T+++S   L ++G
Sbjct: 121 KSLRILGIEK-FTLVGFSYGGMVAFKIAEEYPEMVQAMVVSGSILAMTDTISESNLNQLG 180

Query: 186 YKSWSDFLIPNTVNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRNEQAELLRALVVPD 241
           +KS +D L+P +V G+K+LF +        P  + K ++E M+  R E+AELL ALV+ +
Sbjct: 181 FKSSADLLLPTSVKGLKTLFTLAVHKPMWFPKRLFKDFIEVMITNRKERAELLEALVISN 240

BLAST of Carg22943 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 125.2 bits (313), Expect = 7.3e-29
Identity = 76/229 (33.19%), Postives = 122/229 (53.28%), Query Frame = 0

Query: 22  KVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGNGIMTWQFQVMNLARNY 81
           K +G+R   + ++ GT +NFWV    P+  P P ++L+HG     I  W      L+R +
Sbjct: 20  KRSGLRPVTIDLKDGTVVNFWVSKTKPESKPKPNLLLIHGLGATAIWQWYDVARRLSRYF 79

Query: 82  AVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVLVGFSYGAMVGFRLAEM 141
            +Y+PD +FFG S T R ERS  FQA+ +++ L    V++   LVG SYG  VG+R+A M
Sbjct: 80  NLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVKK-FSLVGLSYGGFVGYRMASM 139

Query: 142 YPDLVAAMVVTSAPTALTESLSRVALQEIG-YKSWSDFLIPNTVNGVKSLFEIGSFDF-- 201
           Y D V  +V+  A   + E   +  + ++      S  L+P +   VK L E+  + F  
Sbjct: 140 YADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPES---VKKLRELMGYIFYK 199

Query: 202 PAL----PNWIMKHYLEAMVKYRN--EQAELLRALVVPDDFTISQFPQV 242
           PAL    P  ++  ++E  +   N  E+ EL++A  +P D  IS+ P++
Sbjct: 200 PALARLVPTCLLHDFIEHALTRDNMEEKRELIKA--IPKDRIISEIPKL 242

BLAST of Carg22943 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 109.4 bits (272), Expect = 4.1e-24
Identity = 67/232 (28.88%), Postives = 117/232 (50.43%), Query Frame = 0

Query: 5   SMANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFW--VPTRNPKKNPNPPVVLLHGF 64
           S  ++      +LR     AG+  + + I+  TT+ FW   P+ + +    P ++LLHGF
Sbjct: 4   SFLSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLLHGF 63

Query: 65  AGNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERP 124
             + +  W  QV  L+  + +YVPD +FFG S +    RS  FQA C+ K + +L VER 
Sbjct: 64  GPSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEVERF 123

Query: 125 CVLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPN 184
            V VG SYG  V + +A+M+P+ V  +V+ S+   L  S +   +         + ++P 
Sbjct: 124 SV-VGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAKCHRIKEVMLPA 183

Query: 185 TVNGVKSLF-EIGSFDFPALPNWIMKHYLEAMV-KYRNEQAELLRALVVPDD 233
           +   ++     + S     +P++++  + + M  + R E+AELL  L +  D
Sbjct: 184 SATDLRRFSGMVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKD 234

BLAST of Carg22943 vs. TAIR 10
Match: AT1G72620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.6e-23
Identity = 63/213 (29.58%), Postives = 120/213 (56.34%), Query Frame = 0

Query: 37  TTMNFWVPTRNPKKNPNPPVVLLHGFAGNGIMTWQF--QVMNLARNYAVYVPDFLFFGES 96
           TT++FW+        PN  +V+LHG+ GN    WQF  QV +L++++ +++PD +FFG+S
Sbjct: 68  TTLHFWISGHRKINRPN--LVMLHGYGGNS--KWQFIHQVSDLSKSFNLFIPDLVFFGKS 127

Query: 97  VTDRAERSPEFQAECVVKGLRRLGV-ERPCVLVGFSYGAMVGFRLAEMYPDLVAAMVVTS 156
            +   +R+ EFQA  +V GL+RLG  +    +   SYG  V +R+A+++P+++  +V+ S
Sbjct: 128 YSRNTDRTIEFQARSIVGGLKRLGCGDGDLSVYSISYGGFVAYRIAKIWPEMIEKLVIVS 187

Query: 157 APTALTESLSRVALQEIGYKSWSDFLIPNTVNGVKSLFEI----GSFDFPALPNWIMKHY 216
           +    T+      +++ G    S+ L+P+    ++ L ++    G      +P++I+  +
Sbjct: 188 SGVGFTQQQKMTEMKKHG-GDVSEILVPSNPRDLRLLVKVSMNTGIRFLDWVPDFILSQF 247

Query: 217 LEAMVK-YRNEQAELLRALV----VPDDFTISQ 238
           +  M +  R E  +L + L+     P+ F+ISQ
Sbjct: 248 IAVMYETNRQELVDLAKNLLEREEEPELFSISQ 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035085.16.4e-136100.00Epoxide hydrolase 4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6605077.11.6e-13199.15Epoxide hydrolase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023533135.11.6e-13198.31epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo][more]
XP_022947943.13.8e-12897.45epoxide hydrolase 4-like [Cucurbita moschata][more]
XP_023006837.15.4e-11195.63epoxide hydrolase 4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8IUS51.3e-0627.61Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2[more]
Q6IE261.3e-0626.17Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1G8061.8e-12897.45epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451675 PE=4 SV=1[more]
A0A6J1KWX22.6e-11195.63epoxide hydrolase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111499509 PE=4 SV=1[more]
A0A5A7TQB41.2e-9067.80Epoxide hydrolase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S4E3103.4e-9067.66uncharacterized protein LOC103499739 OS=Cucumis melo OX=3656 GN=LOC103499739 PE=... [more]
A0A0A0LLY61.8e-8865.82AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247... [more]
Match NameE-valueIdentityDescription
AT4G36610.14.4e-5847.98alpha/beta-Hydrolases superfamily protein [more]
AT2G18360.17.4e-5846.80alpha/beta-Hydrolases superfamily protein [more]
AT1G78210.17.3e-2933.19alpha/beta-Hydrolases superfamily protein [more]
AT5G21950.14.1e-2428.88alpha/beta-Hydrolases superfamily protein [more]
AT1G72620.11.6e-2329.58alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 54..165
e-value: 4.7E-17
score: 62.5
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 8..239
e-value: 4.4E-32
score: 113.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 24..217
NoneNo IPR availablePANTHERPTHR43139SI:DKEY-122A22.2coord: 7..240
NoneNo IPR availablePANTHERPTHR43139:SF22EPOXIDE HYDROLASE 4-LIKEcoord: 7..240

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22943-RACarg22943-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity