Carg22924 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22924
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein NRT1/ PTR FAMILY 5.8-like
LocationCarg_Chr11: 5550310 .. 5554347 (+)
RNA-Seq ExpressionCarg22924
SyntenyCarg22924
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGGAGGAGGAGGAGGAGGAGGAGGGCTGCGGCGGAGACCAAGGCTGAGTGGAGCGTGTATTCTTCTCATAGGTATTTTAGAAGTTTTTTTTTTTTTTTATTTTTTATTTTGAAGTGTTATTAAGGGTTATGACGGAGGTTGGCGGCGCAGTGGTGTCGGGGATGGAGAGATTCGTGTTCAAAGGGGTGGCGTCGAACATGGTGACGTATCTAACAGAGGTGATGAAGATGGGGAACTCGGCGGCGGCGAAAACGGTGAACACCTGGTGCGGCTTCACTTCCATGCTGCCGCTGCTGGTGGCGCCGCTCGCTGATTCTTATTGGGATCGTTATTCCACCATTTTAGCCTCTGCTTTTCTTTACGTCATGGTACCATCACTTACCTCTCTCTTCCCCCAATCTCTATTTTTTTTTGTTTTTTTTTTTAGTATTAATATCTTTAACCAATTGAATTAAAATTGATTGAATTAAAATTGACAAGATTGAAAAGAAAAAATGAGCTGTTATCTCAGATATCTAGAGAGAGGTTTACACCGTAAAGAATGTTTTGTTCTTCTCCTCTTTCGATGTGGGATCTCACATGTTAAAATTTGAATTAAATGAATTGAGTTGGTGTAGGGGTGATCGTGGATTGAATTGTGTCAGATTAAAACATTAATAATAACCCTTATTGAGACATCGAGTTTTCGAACACTCAAGATGACTCATTAGCTCTCTCAAGAGAAATTATTAACATGTTAACTAATTTTTAAATACAAGGTGTGCGGTTAAAATTCAAAATGAGTCGGATTTATTTCGAGTTTTACCATTGATGCTACTTAATGATCAATATACTTAAAAAATCTCTACTTTTCTTTAAATTTGGTTGAAAATTAAAAATAAAAAAAAATAAATAATTAAGTTTTAAAAAAATAAAACGTTAAATATGATACATAAAAACTTTATTGGTTCCACAATTTTGCTTCTAGTTTTTAACTTCTAAAATTAAATAATATAATAATAATAAATAAATAAATAAAAAATAATCAGGTTCTGCTTTTGTTATTATGGTGATGACTAAGAATAGGGGGTGCAATATTTAGACAATTTTGTCCAATTATATTAACTTTCTCTTTTCATAAGAGTAAGTGCTTTGCCTTTTTCTCCCACCTTATTTAGGCCATAAAGATTTATTATTATTATTATTATTATTATTATTTTGAGTTTAATATCATGTCGGATGGAGATTTGAACCTATAAACCTTCTTGATCGGGTAAGATGTTTTGTATAATTTAAACCCTAAATTGGTAATATTGAGGTGGGTAACTTTTTAAGTGACCAAATTTTATGCAGGGGTTAGTTGCTTTAACATCAACGACATTAGCACGATCATGGTCACCAGCAAACACAGCCTCCTCCTTCTTATTCTCGTCCCTCTACCTCATCTCACTCGGCCAGGGCGGATACAACCCATCTCTCCAAGCCTTTGGTGCTGACCAGCTCGACCATGACGTTGCCGAACTCCCGATTGACATGGCCAAAACACCGTCCTCCTCCGAGCATAAGGCAAAGAAGAAGAGCTTGTTCTTCCAATGGTGGTACTTTGGAGTGTGCAGTGGCTGCCTCCTTGGTGTGACGGTCATGTCCTACATTCAAGACACATTTGGTTGGGTGATTGGGTTTGCCATCCCCATGGGCGCCATGGTTTGTTCTGTGGCGGTTTTTGCTTGTGGCACTAAGATTTATCGGTATAAGATAGAGGAGGAAGAGGATGGGGTTGCGAAGAGAGGGCTTGTTATCATGGTGGTGGAGCTTGTCAAAGCCTCGGCTTCTAGGCTCGTTTGTGCTACAAATGTTGTTGCCTTGTCTAAGAACAAGCCTCGTGATGATGTTGAGTTTGAGTAAGAACATCTCTATATCTCTCTTTGTTATTTTTTTTTTTAAAATTTTATTGCCTATCTTTTTGAAATTTTGATTTCGTTTTTAGAACCCGTGTGAAATTCAAATGTGTTTGTAAGGAAGGTGAAAAACTTATTCGAAAAATTGTGAGCAAATTATCAAACGAGTTTTTAACTCTTAGTTTTTGTGGCATTTGCAACCGTGTAACTGGGCATACTGCTAGTAGATATTGTCCTATTTGGGTTTTCCTATCGGGCTTCCCTTTAAGGTTTTTAAAACACATCTTCTAGAGAGAGGTTTCTACACTCTTATAAAGAATGTTTCGTTCTTCTCCCAAACCGAAGTGGGATCTGCACTCATTCCCCAACTGTAACAACTGCTCAAACTCACTACTAATAGATTTTGAACTTTCTCTTTCGGGCTTTCCCTTAAAGTTTTAAAATGTGTTTCTAGGGAGAGGTTTTCACACTTTTATAAAAAATGTTTCGTTCTCCTCCTCTCTAACCGTTGTGGGATCTTACAAACAACAGTTTCTTAATTGTTGATGTGATACGTGTTGACTAATCAATAGGTGATGATTTGACATAGCATTAGAAAAGATGACCAGGATCAAAAAGGGCGAGTAGATCGACCAAAGAAAAAAAAAACCCTCAACTTACCTATACATTTATGTTTATTTTTTCTTTCGGTTTAACCCGCCCTTAAATTTGTGTCAAAAATCTTTGAAAATGAACCCGAGATAAAATGAACCCGGGAAGAACTTGATGAGTGAGCTAAAGGATGAATATGAAGCTGATTATTTGACATTTTGCAGGCTGCAAGAGAGCAAGCCACTCTGCCATGAAAGCTCAGGAGCCATGAAATCCATGGAAGAAAACACCAACATGATTCCAAAAGAAAGATTATGTGTGGCCGACAAGGTGAAGCTGATTTTGCGGCTGCTACCAATTTGGACATTGCTTCTAATGTTCGCTGTAATATTCCAACAACCAGCTACCTTCTTCACCAAACAAGGCATGACAATGGAGCGGAACATCGGCGCCAACTTCAAGATCCCGCCCGCCACCCTCCAGAGCTCCATCACCATCTCCATAATCCTCCTCATGCCGTTGTACGACAACGTTTTGATCCCGATCACCCGCCTCTTCACCCGCACTGAAAAGGGCATCACTGTCATGCAGAGGATGGGCATTGGCATGTTCCTCTCTACCATTGCCATGATCCTCGCTGCCCTCGTCGAAGCCAAGAGGTTGACAATGACTAAAACCACATCGTCATTGTCGTATGCGCCACTTAGTATCTTCTGGCTGTTGCCTCAGTACATAATATTAGGAGTTTCAGACATCTTTACAGTGGTAGGGATGCAAGAGTTCTTCTACTCAGAGGTTCCCGTGAGCATGAGAACGACAGCATTTGCGCTGTACAACAGCGTGTTCGGTGTCGGGAGCTTTTGCAGTGCAATAATGATATCAATCGTGGAGTTATTGACAAGTTCGGATGGGAGGCCGAACTGGTTTTCGGATGACATGAGCAAAGCTAGGTTGGACAAGTATTATTGGTTGTTAGCATTTTGCAGTGGGTTGAGCTTTGTATTGTATGTGATTTGGTGTAAATGTTGCAGAAACAGGATCAGTGAAGAAGAAACACAATATTGAATTTGAATCAAGCTTTATGAGTTTGAATGGAATTGTGGAGTGAGGTTCTAACTAGTTTTTCTGTTCACTGTTCACAGTTTCGAACTTCGAACCTAGCTCACCTTTTGATAAAGAGTATAATGTTTTAACCAATAGAGTTATGCTCGGGTTGGTATAACTTCTTAAACTTATATCAACTTGCTATTAACCGGTAGTTGTGATGCTAATGTGGGGTAGGCATTTCAACAGTTGAAATAACGCTTTCAATCACTACAGAGAATTTATAAGATAGCTAACCAGCCCAAGTCTACCACTAACAGATATTGCCCTTATAAATAATGCTTCATTCTCCTCTCCAACCAATATAGGATCTCACAATCCACCCCTCTGGAGGGCCAGTGTCCTCGCTGGCACACCGCCCGATGCTTGACTCTGATACCATTGGGTACAGGCCAAGCCCATCGCTAGCAGATATTGTCCTCTTTATGCTTTCTCTTCCAGACTTTCTCACAAGATTTTAA

mRNA sequence

ATGTCGGGAGGAGGAGGAGGAGGAGGAGGGCTGCGGCGGAGACCAAGGCTGAGTGGAGCGTGTATTCTTCTCATAGTGGTGTCGGGGATGGAGAGATTCGTGTTCAAAGGGGTGGCGTCGAACATGGTGACGTATCTAACAGAGGTGATGAAGATGGGGAACTCGGCGGCGGCGAAAACGGTGAACACCTGGTGCGGCTTCACTTCCATGCTGCCGCTGCTGGTGGCGCCGCTCGCTGATTCTTATTGGGATCGTTATTCCACCATTTTAGCCTCTGCTTTTCTTTACGTCATGGGGTTAGTTGCTTTAACATCAACGACATTAGCACGATCATGGTCACCAGCAAACACAGCCTCCTCCTTCTTATTCTCGTCCCTCTACCTCATCTCACTCGGCCAGGGCGGATACAACCCATCTCTCCAAGCCTTTGGTGCTGACCAGCTCGACCATGACGTTGCCGAACTCCCGATTGACATGGCCAAAACACCGTCCTCCTCCGAGCATAAGGCAAAGAAGAAGAGCTTGTTCTTCCAATGGTGGTACTTTGGAGTGTGCAGTGGCTGCCTCCTTGGTGTGACGGTCATGTCCTACATTCAAGACACATTTGGTTGGGTGATTGGGTTTGCCATCCCCATGGGCGCCATGGTTTGTTCTGTGGCGGTTTTTGCTTGTGGCACTAAGATTTATCGGTATAAGATAGAGGAGGAAGAGGATGGGGTTGCGAAGAGAGGGCTTGTTATCATGGTGGTGGAGCTTGTCAAAGCCTCGGCTTCTAGGCTCGTTTGTGCTACAAATGTTGTTGCCTTGTCTAAGAACAAGCCTCGTGATGATGTTGAGTTTGAGCTGCAAGAGAGCAAGCCACTCTGCCATGAAAGCTCAGGAGCCATGAAATCCATGGAAGAAAACACCAACATGATTCCAAAAGAAAGATTATGTGTGGCCGACAAGGTGAAGCTGATTTTGCGGCTGCTACCAATTTGGACATTGCTTCTAATGTTCGCTGTAATATTCCAACAACCAGCTACCTTCTTCACCAAACAAGGCATGACAATGGAGCGGAACATCGGCGCCAACTTCAAGATCCCGCCCGCCACCCTCCAGAGCTCCATCACCATCTCCATAATCCTCCTCATGCCGTTGTACGACAACGTTTTGATCCCGATCACCCGCCTCTTCACCCGCACTGAAAAGGGCATCACTGTCATGCAGAGGATGGGCATTGGCATGTTCCTCTCTACCATTGCCATGATCCTCGCTGCCCTCGTCGAAGCCAAGAGGTTGACAATGACTAAAACCACATCGTCATTGTCGTATGCGCCACTTAGTATCTTCTGGCTGTTGCCTCAGTACATAATATTAGGAGTTTCAGACATCTTTACAGTGGTAGGGATGCAAGAGTTCTTCTACTCAGAGGTTCCCGTGAGCATGAGAACGACAGCATTTGCGCTGTACAACAGCGTGTTCGGTGTCGGGAGCTTTTGCAGTGCAATAATGATATCAATCGTGGAGTTATTGACAAGTTCGGATGGGAGGCCGAACTGGTTTTCGGATGACATGAGCAAAGCTAGGTTGGACAAGTATTATTGGTTGTTAGCATTTTGCAGTGGGTTGAGCTTTGATCTCACAATCCACCCCTCTGGAGGGCCAGTGTCCTCGCTGGCACACCGCCCGATGCTTGACTCTGATACCATTGGGTACAGGCCAAGCCCATCGCTAGCAGATATTGTCCTCTTTATGCTTTCTCTTCCAGACTTTCTCACAAGATTTTAA

Coding sequence (CDS)

ATGTCGGGAGGAGGAGGAGGAGGAGGAGGGCTGCGGCGGAGACCAAGGCTGAGTGGAGCGTGTATTCTTCTCATAGTGGTGTCGGGGATGGAGAGATTCGTGTTCAAAGGGGTGGCGTCGAACATGGTGACGTATCTAACAGAGGTGATGAAGATGGGGAACTCGGCGGCGGCGAAAACGGTGAACACCTGGTGCGGCTTCACTTCCATGCTGCCGCTGCTGGTGGCGCCGCTCGCTGATTCTTATTGGGATCGTTATTCCACCATTTTAGCCTCTGCTTTTCTTTACGTCATGGGGTTAGTTGCTTTAACATCAACGACATTAGCACGATCATGGTCACCAGCAAACACAGCCTCCTCCTTCTTATTCTCGTCCCTCTACCTCATCTCACTCGGCCAGGGCGGATACAACCCATCTCTCCAAGCCTTTGGTGCTGACCAGCTCGACCATGACGTTGCCGAACTCCCGATTGACATGGCCAAAACACCGTCCTCCTCCGAGCATAAGGCAAAGAAGAAGAGCTTGTTCTTCCAATGGTGGTACTTTGGAGTGTGCAGTGGCTGCCTCCTTGGTGTGACGGTCATGTCCTACATTCAAGACACATTTGGTTGGGTGATTGGGTTTGCCATCCCCATGGGCGCCATGGTTTGTTCTGTGGCGGTTTTTGCTTGTGGCACTAAGATTTATCGGTATAAGATAGAGGAGGAAGAGGATGGGGTTGCGAAGAGAGGGCTTGTTATCATGGTGGTGGAGCTTGTCAAAGCCTCGGCTTCTAGGCTCGTTTGTGCTACAAATGTTGTTGCCTTGTCTAAGAACAAGCCTCGTGATGATGTTGAGTTTGAGCTGCAAGAGAGCAAGCCACTCTGCCATGAAAGCTCAGGAGCCATGAAATCCATGGAAGAAAACACCAACATGATTCCAAAAGAAAGATTATGTGTGGCCGACAAGGTGAAGCTGATTTTGCGGCTGCTACCAATTTGGACATTGCTTCTAATGTTCGCTGTAATATTCCAACAACCAGCTACCTTCTTCACCAAACAAGGCATGACAATGGAGCGGAACATCGGCGCCAACTTCAAGATCCCGCCCGCCACCCTCCAGAGCTCCATCACCATCTCCATAATCCTCCTCATGCCGTTGTACGACAACGTTTTGATCCCGATCACCCGCCTCTTCACCCGCACTGAAAAGGGCATCACTGTCATGCAGAGGATGGGCATTGGCATGTTCCTCTCTACCATTGCCATGATCCTCGCTGCCCTCGTCGAAGCCAAGAGGTTGACAATGACTAAAACCACATCGTCATTGTCGTATGCGCCACTTAGTATCTTCTGGCTGTTGCCTCAGTACATAATATTAGGAGTTTCAGACATCTTTACAGTGGTAGGGATGCAAGAGTTCTTCTACTCAGAGGTTCCCGTGAGCATGAGAACGACAGCATTTGCGCTGTACAACAGCGTGTTCGGTGTCGGGAGCTTTTGCAGTGCAATAATGATATCAATCGTGGAGTTATTGACAAGTTCGGATGGGAGGCCGAACTGGTTTTCGGATGACATGAGCAAAGCTAGGTTGGACAAGTATTATTGGTTGTTAGCATTTTGCAGTGGGTTGAGCTTTGATCTCACAATCCACCCCTCTGGAGGGCCAGTGTCCTCGCTGGCACACCGCCCGATGCTTGACTCTGATACCATTGGGTACAGGCCAAGCCCATCGCTAGCAGATATTGTCCTCTTTATGCTTTCTCTTCCAGACTTTCTCACAAGATTTTAA

Protein sequence

MSGGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASSFLFSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYFGVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAKRGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEENTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIPPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALVEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFDLTIHPSGGPVSSLAHRPMLDSDTIGYRPSPSLADIVLFMLSLPDFLTRF
Homology
BLAST of Carg22924 vs. NCBI nr
Match: KAG7022250.1 (Protein NRT1/ PTR FAMILY 5.8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MSGGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKT 60
           MSGGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKT
Sbjct: 1   MSGGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKT 60

Query: 61  VNTWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASS 120
           VNTWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASS
Sbjct: 61  VNTWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASS 120

Query: 121 FLFSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWW 180
           FLFSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWW
Sbjct: 121 FLFSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWW 180

Query: 181 YFGVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGV 240
           YFGVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGV
Sbjct: 181 YFGVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGV 240

Query: 241 AKRGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSME 300
           AKRGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSME
Sbjct: 241 AKRGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSME 300

Query: 301 ENTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFK 360
           ENTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFK
Sbjct: 301 ENTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFK 360

Query: 361 IPPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAA 420
           IPPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAA
Sbjct: 361 IPPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAA 420

Query: 421 LVEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTA 480
           LVEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTA
Sbjct: 421 LVEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTA 480

Query: 481 FALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFD 540
           FALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFD
Sbjct: 481 FALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFD 540

Query: 541 LTIHPSGGPVSSLAHRPMLDSDTIGYRPSPSLADIVLFMLSLPDFLTRF 590
           LTIHPSGGPVSSLAHRPMLDSDTIGYRPSPSLADIVLFMLSLPDFLTRF
Sbjct: 541 LTIHPSGGPVSSLAHRPMLDSDTIGYRPSPSLADIVLFMLSLPDFLTRF 589

BLAST of Carg22924 vs. NCBI nr
Match: XP_022932208.1 (protein NRT1/ PTR FAMILY 5.8-like [Cucurbita moschata])

HSP 1 Score: 1027.7 bits (2656), Expect = 3.9e-296
Identity = 533/541 (98.52%), Postives = 534/541 (98.71%), Query Frame = 0

Query: 3   GGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVN 62
           GGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVN
Sbjct: 5   GGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVN 64

Query: 63  TWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASSFL 122
           TWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTST LARSWSPANT SSFL
Sbjct: 65  TWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTALARSWSPANTTSSFL 124

Query: 123 FSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYF 182
           FSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPID  KTPSSSEHKAKKK LFFQWWYF
Sbjct: 125 FSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDTPKTPSSSEHKAKKKRLFFQWWYF 184

Query: 183 GVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAK 242
           GVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAK
Sbjct: 185 GVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAK 244

Query: 243 RGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEEN 302
           RGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEEN
Sbjct: 245 RGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEEN 304

Query: 303 TNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIP 362
           TNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIP
Sbjct: 305 TNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIP 364

Query: 363 PATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALV 422
           PATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALV
Sbjct: 365 PATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALV 424

Query: 423 EAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFA 482
           EAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFA
Sbjct: 425 EAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFA 484

Query: 483 LYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFDLT 542
           LYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSF L 
Sbjct: 485 LYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFVLY 544

Query: 543 I 544
           +
Sbjct: 545 V 545

BLAST of Carg22924 vs. NCBI nr
Match: XP_023531217.1 (protein NRT1/ PTR FAMILY 5.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1012.7 bits (2617), Expect = 1.3e-291
Identity = 528/545 (96.88%), Postives = 531/545 (97.43%), Query Frame = 0

Query: 3   GGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVN 62
           GGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVN
Sbjct: 5   GGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVN 64

Query: 63  TWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASSFL 122
           TWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTST LARSWSPANT SSFL
Sbjct: 65  TWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTALARSWSPANTTSSFL 124

Query: 123 FSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEH----KAKKKSLFFQ 182
           FSSLYLISLGQGGYNPSLQAFGADQLDHDV ELP D AKTPSSSE     K KKKSLFFQ
Sbjct: 125 FSSLYLISLGQGGYNPSLQAFGADQLDHDVVELPTDTAKTPSSSEQKPKKKKKKKSLFFQ 184

Query: 183 WWYFGVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEED 242
           WWYFGVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIY+YKIEEEED
Sbjct: 185 WWYFGVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYQYKIEEEED 244

Query: 243 GVAKRGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKS 302
           GV KRGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKS
Sbjct: 245 GVVKRGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKS 304

Query: 303 MEENTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGAN 362
           MEEN+NMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGAN
Sbjct: 305 MEENSNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGAN 364

Query: 363 FKIPPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMIL 422
           FKIPPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMIL
Sbjct: 365 FKIPPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMIL 424

Query: 423 AALVEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRT 482
           AALVEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRT
Sbjct: 425 AALVEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRT 484

Query: 483 TAFALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLS 542
           TAFALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLS
Sbjct: 485 TAFALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLS 544

Query: 543 FDLTI 544
           F L +
Sbjct: 545 FVLYV 549

BLAST of Carg22924 vs. NCBI nr
Match: XP_022970676.1 (protein NRT1/ PTR FAMILY 5.8-like [Cucurbita maxima])

HSP 1 Score: 968.8 bits (2503), Expect = 2.2e-278
Identity = 506/542 (93.36%), Postives = 520/542 (95.94%), Query Frame = 0

Query: 5   GGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTW 64
           GGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLT+VMKMGNSAAAKTVNTW
Sbjct: 3   GGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTDVMKMGNSAAAKTVNTW 62

Query: 65  CGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASSFLFS 124
           CGFTSMLPLLVAPLADSYWDRYST+LASAFLYVMGLVALTSTTLA+SWSP NT SSFLFS
Sbjct: 63  CGFTSMLPLLVAPLADSYWDRYSTVLASAFLYVMGLVALTSTTLAQSWSPTNTTSSFLFS 122

Query: 125 SLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEH--KAKKKSLFFQWWYF 184
           SLYLISLGQGGYNPSLQAFGADQLDHDV ELP D AKT SSSE   K KKKSLFFQWWYF
Sbjct: 123 SLYLISLGQGGYNPSLQAFGADQLDHDVVELPTDTAKTLSSSEQKPKKKKKSLFFQWWYF 182

Query: 185 GVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAK 244
           GVCSGCLLGVTVMSYIQDT GWVIGFAIPMGAMVCSVAVFACG+KIYRYK+ EEEDGV K
Sbjct: 183 GVCSGCLLGVTVMSYIQDTLGWVIGFAIPMGAMVCSVAVFACGSKIYRYKV-EEEDGVEK 242

Query: 245 RGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFEL-QESKPLCHESSGAMKSMEE 304
           RGLVI VVELVKA+ASRL+CATNVVALSKNKPRDDVEFEL QESKPLCHESSG MKSMEE
Sbjct: 243 RGLVIKVVELVKATASRLICATNVVALSKNKPRDDVEFELQQESKPLCHESSGTMKSMEE 302

Query: 305 NTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKI 364
           N++MIPKER+CVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERN+ ANFKI
Sbjct: 303 NSDMIPKERICVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNVSANFKI 362

Query: 365 PPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAAL 424
           PPATLQSSITISIILLMPLYDN+LIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAAL
Sbjct: 363 PPATLQSSITISIILLMPLYDNILIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAAL 422

Query: 425 VEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAF 484
           VEAKRLTMTKTTSSLSY PLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAF
Sbjct: 423 VEAKRLTMTKTTSSLSYVPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAF 482

Query: 485 ALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFDL 544
           ALYNSVFGVGSFCSAIMISIVELLTSSDG  +WFSDDMSKARLDKYYWLLAFCSGLSF L
Sbjct: 483 ALYNSVFGVGSFCSAIMISIVELLTSSDGSLSWFSDDMSKARLDKYYWLLAFCSGLSFVL 542

BLAST of Carg22924 vs. NCBI nr
Match: KAG6588403.1 (Protein NRT1/ PTR FAMILY 5.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 959.1 bits (2478), Expect = 1.7e-275
Identity = 498/498 (100.00%), Postives = 498/498 (100.00%), Query Frame = 0

Query: 26  VVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFTSMLPLLVAPLADSYWDR 85
           VVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFTSMLPLLVAPLADSYWDR
Sbjct: 8   VVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFTSMLPLLVAPLADSYWDR 67

Query: 86  YSTILASAFLYVMGLVALTSTTLARSWSPANTASSFLFSSLYLISLGQGGYNPSLQAFGA 145
           YSTILASAFLYVMGLVALTSTTLARSWSPANTASSFLFSSLYLISLGQGGYNPSLQAFGA
Sbjct: 68  YSTILASAFLYVMGLVALTSTTLARSWSPANTASSFLFSSLYLISLGQGGYNPSLQAFGA 127

Query: 146 DQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYFGVCSGCLLGVTVMSYIQDTFGWV 205
           DQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYFGVCSGCLLGVTVMSYIQDTFGWV
Sbjct: 128 DQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYFGVCSGCLLGVTVMSYIQDTFGWV 187

Query: 206 IGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAKRGLVIMVVELVKASASRLVCATN 265
           IGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAKRGLVIMVVELVKASASRLVCATN
Sbjct: 188 IGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAKRGLVIMVVELVKASASRLVCATN 247

Query: 266 VVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEENTNMIPKERLCVADKVKLILRLLP 325
           VVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEENTNMIPKERLCVADKVKLILRLLP
Sbjct: 248 VVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEENTNMIPKERLCVADKVKLILRLLP 307

Query: 326 IWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIPPATLQSSITISIILLMPLYDNVL 385
           IWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIPPATLQSSITISIILLMPLYDNVL
Sbjct: 308 IWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIPPATLQSSITISIILLMPLYDNVL 367

Query: 386 IPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALVEAKRLTMTKTTSSLSYAPLSIFW 445
           IPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALVEAKRLTMTKTTSSLSYAPLSIFW
Sbjct: 368 IPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALVEAKRLTMTKTTSSLSYAPLSIFW 427

Query: 446 LLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFALYNSVFGVGSFCSAIMISIVELL 505
           LLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFALYNSVFGVGSFCSAIMISIVELL
Sbjct: 428 LLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFALYNSVFGVGSFCSAIMISIVELL 487

Query: 506 TSSDGRPNWFSDDMSKAR 524
           TSSDGRPNWFSDDMSKAR
Sbjct: 488 TSSDGRPNWFSDDMSKAR 505

BLAST of Carg22924 vs. ExPASy Swiss-Prot
Match: Q9LFR1 (Protein NRT1/ PTR FAMILY 5.8 OS=Arabidopsis thaliana OX=3702 GN=NPF5.8 PE=2 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 3.4e-165
Identity = 332/542 (61.25%), Postives = 398/542 (73.43%), Query Frame = 0

Query: 9   GGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFT 68
           GG +RR  LS +C LLIV++G+ER+ FKGVASN+VTYLT+V+KM NS AA TVNTW GFT
Sbjct: 3   GGEKRR-GLSKSCALLIVIAGIERYAFKGVASNLVTYLTDVVKMSNSRAATTVNTWSGFT 62

Query: 69  SMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTAS-SFLFSSLY 128
            MLPL  AP ADSYWDR+ TILAS+ LY +GLV LT T  A S S   T S  FL++SL 
Sbjct: 63  FMLPLFSAPFADSYWDRFFTILASSSLYFVGLVGLTFTAFAGSRSTTKTISLYFLYTSLS 122

Query: 129 LISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSS--SEHKAKKKSLFFQWWYFGVC 188
           L++LG G  NPSLQAFGADQLD+D   L  D    PSS   E K+ +K+ FFQWWYFGVC
Sbjct: 123 LVALGLGVLNPSLQAFGADQLDYD---LDHDNDHEPSSENKEVKSNRKTQFFQWWYFGVC 182

Query: 189 SGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAKRGL 248
           +G LLGVTVM+YIQDTFGWVIGFAIP  +M+  + +F CG  +Y Y      D   K   
Sbjct: 183 AGSLLGVTVMAYIQDTFGWVIGFAIPTASMLLLIFLFLCGCGVYVY-----ADPDLKAKP 242

Query: 249 VIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCH----ESSGAMKSMEE 308
              ++E++K      VC  N + L  +   + +E ELQ+ KPLC+    E++   KS+ +
Sbjct: 243 FQRILEIIK----ERVCGRNKITLVNDHDLNAMELELQDQKPLCNCSNTEANTTTKSLPD 302

Query: 309 NTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKI 368
           +     K      + VKL+LRLLPIWT+LLMFAVIFQQPATFFTKQGMTM+RNIG NFKI
Sbjct: 303 DHKSC-KTGFSGLETVKLLLRLLPIWTMLLMFAVIFQQPATFFTKQGMTMKRNIGPNFKI 362

Query: 369 PPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAAL 428
           PPATLQS+IT+SIILLMP YD +LIPI +  T+ EKGI+V +RMGIGMFLS IA+++AAL
Sbjct: 363 PPATLQSTITLSIILLMPFYDKILIPIAKKLTKNEKGISVKERMGIGMFLSIIAIVIAAL 422

Query: 429 VEAKRL---TMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRT 488
           VE KRL    M KTT +L   P+SI WLLPQYI+LG+SDIFTVVGMQEFFYSEVPVSMRT
Sbjct: 423 VERKRLKISKMMKTTPNLD--PVSILWLLPQYILLGISDIFTVVGMQEFFYSEVPVSMRT 482

Query: 489 TAFALYNSVFGVGSFCSAIMISIVELLTSS-DGRPNWFSDDMSKARLDKYYWLLAFCSGL 540
             FALY SVFGVGSF SA +ISI+E  TSS  G+ NWF+DDMS+ARLD YYWLLAF S +
Sbjct: 483 MGFALYTSVFGVGSFVSAALISIIETYTSSRGGKHNWFADDMSEARLDNYYWLLAFTSAI 528

BLAST of Carg22924 vs. ExPASy Swiss-Prot
Match: Q9SRI2 (Protein NRT1/ PTR FAMILY 5.9 OS=Arabidopsis thaliana OX=3702 GN=NPF5.9 PE=2 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 4.7e-159
Identity = 317/537 (59.03%), Postives = 391/537 (72.81%), Query Frame = 0

Query: 17  LSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFTSMLPLLVA 76
           LS +C LLIV++GMER+ FKGVASN+VTYLT+V+KM NS AAKTVNTW GFTSMLPL  A
Sbjct: 10  LSKSCALLIVIAGMERYAFKGVASNLVTYLTDVVKMSNSRAAKTVNTWAGFTSMLPLFSA 69

Query: 77  PLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASS-FLFSSLYLISLGQGG 136
           PLAD+YWDR+ TILAS+ +Y +GLV LT T  A S S   T SS FL+SSL L+S+G G 
Sbjct: 70  PLADTYWDRFFTILASSSVYFVGLVGLTWTAFAGSRSATKTISSYFLYSSLCLVSIGLGV 129

Query: 137 YNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYFGVCSGCLLGVTVM 196
            NPSLQAFGADQLDHD+ +   D++ +    + KA +K+ FFQ WYFGVC+G L+GVTVM
Sbjct: 130 LNPSLQAFGADQLDHDL-DKNFDLS-SGDQKDAKATRKTQFFQLWYFGVCTGSLMGVTVM 189

Query: 197 SYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAKRGLVIMVVELVKA 256
           +YIQDTFGWV+GFAIP   +  S+ VF  G  IY Y        + K+       +++K 
Sbjct: 190 AYIQDTFGWVLGFAIPGIVIFLSILVFMSGCGIYVYAPGAR---LKKKTTTTPFEKILKF 249

Query: 257 SASRLVCATNVVALSKNKPRDDVEFELQESKPLC-------HESSGAMKSMEENTNMIPK 316
              R+V   ++  L+  K  D +E EL+E +PLC          S   K +E++ +   K
Sbjct: 250 IKGRVVKQRSIYTLADEKDLDAMELELEE-RPLCKCETEDIETPSTTSKGLEDDES--SK 309

Query: 317 ERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIPPATLQS 376
                 D VKL++RL PIW +LLMFAVIFQ PATFFTKQG+TM+RNIG+NFKIPPATLQS
Sbjct: 310 TVFSGIDNVKLVIRLFPIWMMLLMFAVIFQLPATFFTKQGVTMKRNIGSNFKIPPATLQS 369

Query: 377 SITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALVEAKRLT 436
           +IT+SIILLMPLYD +LIPIT+   +   GI+VM+RMG+GMFLS IA+++AA+VE KRL 
Sbjct: 370 TITLSIILLMPLYDKILIPITKRIKKNGTGISVMERMGVGMFLSIIAIVIAAIVERKRLA 429

Query: 437 MTKTTSSL-----SYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFAL 496
           +++   +L        PLSIFWLLPQYI+LG+SDIFTVVGMQEFFYSEVPV MRT  FAL
Sbjct: 430 ISQKMKTLPDYDPETVPLSIFWLLPQYILLGISDIFTVVGMQEFFYSEVPVRMRTMGFAL 489

Query: 497 YNSVFGVGSFCSAIMISIVELLTSSDG-RPNWFSDDMSKARLDKYYWLLAFCSGLSF 540
           Y SVFGVGSF SA +ISIVE  +SS G R NWF+DDMS+ARLDKYYWLLA  S +SF
Sbjct: 490 YTSVFGVGSFVSAALISIVEAYSSSTGDRQNWFADDMSEARLDKYYWLLALTSTISF 538

BLAST of Carg22924 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 3.3e-104
Identity = 222/526 (42.21%), Postives = 310/526 (58.94%), Query Frame = 0

Query: 24  LIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFTSMLPLLVAPLADSYW 83
           +I V   ERF + G++SN++TYLT  +    +AAA  VN W G  S+LPLL A +ADS+ 
Sbjct: 40  IIGVEVAERFAYYGISSNLITYLTGPLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFL 99

Query: 84  DRYSTILASAFLYVMGLVALT----------STTLARSWSPANTASSFLFSSLYLISLGQ 143
            R+ TILA++ LY++GL  LT           + L  S SP     +F FS+LYL++L Q
Sbjct: 100 GRFRTILAASALYIVGLGVLTLSAMIPSDCKVSNLLSSCSPRFQVITF-FSALYLVALAQ 159

Query: 144 GGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYFGVCSGCLLGVT 203
           GG+ P +QAFGADQ D    E              + K KS FF WWYFG+C G L  + 
Sbjct: 160 GGHKPCVQAFGADQFDEKEPE--------------ECKAKSSFFNWWYFGMCFGTLTTLW 219

Query: 204 VMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAKRGLVIMVVELV 263
           V++YIQD   W +GF IP  AMV ++ V   GT  YR+ I  E+     R   + V  + 
Sbjct: 220 VLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQSPFVRIGNVYVAAVK 279

Query: 264 KASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEENTNMIPKERLCV 323
             S S L  A     L         +F       +    S ++  +EE            
Sbjct: 280 NWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNGSCSIDELEE------------ 339

Query: 324 ADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIPPATLQSSITIS 383
               K +LRL PIW   L++AV+F Q  TFFTKQG TMER+I   +KI PATLQS I++S
Sbjct: 340 ---AKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKISPATLQSFISLS 399

Query: 384 IILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALVEAKRLTMTK-- 443
           I++ +P+YD VLIPI R FT    GIT++QR+G G+FLS +AM++AALVE KRL      
Sbjct: 400 IVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAALVEMKRLKTAADY 459

Query: 444 --TTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFALYNSVFG 503
               S  +  P+S++WL+PQY++ G++D+F +VG+QEFFY +VP  +R+   ALY S+FG
Sbjct: 460 GLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELRSVGLALYLSIFG 519

Query: 504 VGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCS 536
           +G+F S+ MISI+E  TS  G+ +WF++++++A LD +YWLLA  S
Sbjct: 520 IGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLS 535

BLAST of Carg22924 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 354.8 bits (909), Expect = 1.9e-96
Identity = 211/533 (39.59%), Postives = 314/533 (58.91%), Query Frame = 0

Query: 20  ACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFTSMLPLLVAPLA 79
           A + +I V   ERF + G+ SN+++YLT  +    + AA  VN W G  ++LP+L A +A
Sbjct: 35  AALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAVAAANVNAWSGIATLLPVLGAFVA 94

Query: 80  DSYWDRYSTILASAFLYVMGLVALTSTT--LARSWSPANTASSFL----FSSLYLISLGQ 139
           D++  RY TI+ S+ +YV+GL  LT +   +  +    ++ SSFL    F SLYL+++GQ
Sbjct: 95  DAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEVTSSTSSFLNVLFFFSLYLVAIGQ 154

Query: 140 GGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYFGVCSGCLLGVT 199
            G+ P +QAFGADQ D                S+ K+ + S FF WWY  + +G    + 
Sbjct: 155 SGHKPCVQAFGADQFDE-------------KDSQEKSDRSS-FFNWWYLSLSAGICFAIL 214

Query: 200 VMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRY---KIEEEEDGVAKRGLVIMVV 259
           V+ YIQ+ F W  GF IP   MV S+ +F  G +IYRY   + EEE +   + G V  V 
Sbjct: 215 VVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRIGRVFFVA 274

Query: 260 ELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEENTNMIPKER 319
              +  +S  +C    V L  N   +   F   ++  + ++SS    + + +        
Sbjct: 275 LKNQRLSSSDLCK---VELEANTSPEKQSF-FNKALLVPNDSSQGENASKSSD------- 334

Query: 320 LCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIPPATLQSSI 379
             V D   LI RL+P+W   L +A+ + Q  TFFTKQG+TM+R I    KIPPA+LQ  I
Sbjct: 335 --VEDATALI-RLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASLQVFI 394

Query: 380 TISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALVEAKRLTMT 439
            ISI+L +P+YD V +PI RL T+   GIT ++R+G G+ LSTI M++AALVE KRL   
Sbjct: 395 GISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRLETA 454

Query: 440 KTTSSL----SYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFALYNS 499
           K    +    +  P+SI+WL+PQY++LG++D++T+VGMQEFFYS+VP  +R+   ALY S
Sbjct: 455 KEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLALYLS 514

Query: 500 VFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSF 540
             GVGS  S+++IS+++L T  D   +WF+ ++++A LD +YWLLA  S + F
Sbjct: 515 ALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGF 539

BLAST of Carg22924 vs. ExPASy Swiss-Prot
Match: Q8RX67 (Protein NRT1/ PTR FAMILY 5.11 OS=Arabidopsis thaliana OX=3702 GN=NPF5.11 PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 4.3e-96
Identity = 210/542 (38.75%), Postives = 314/542 (57.93%), Query Frame = 0

Query: 9   GGLRRRPRLSG---ACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWC 68
           G L  R   SG   +  L+IVV   ERF + G+ASN++ YLT  +    +AAA  VN W 
Sbjct: 17  GFLEIRENTSGGWKSARLIIVVQMAERFAYFGIASNLIMYLTGPLGESTAAAAANVNAWT 76

Query: 69  GFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANT---ASSFL 128
           G  + LPLL   LADSY  R+ TI+ S+ LY++GL  L+ +T+  S    ++     +  
Sbjct: 77  GTVAFLPLLGGFLADSYLGRFRTIIISSSLYILGLGLLSFSTMIPSHQSKDSNQLQETIF 136

Query: 129 FSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHK-AKKKSLFFQWWY 188
           F SLYL+++GQGGYNP ++ FGADQ D                ++HK A+ KS FF W  
Sbjct: 137 FFSLYLVAIGQGGYNPCIKVFGADQFD---------------GNDHKEARDKSSFFNWLM 196

Query: 189 FGVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVA 248
           FG C   L    V +YIQ+   W +GF IP  +M+ S+ +F  GT  YR+  E     V 
Sbjct: 197 FGNCISILTTRLVSTYIQENLSWSLGFGIPSVSMLLSLFLFLLGTTSYRFSTER----VG 256

Query: 249 KRGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKP----LCHESSGAMK 308
           K+           A  SR+      +   KN+ + D++     +      L H+SS   +
Sbjct: 257 KKNPF--------ARISRV-----FMEALKNRRQPDLDIANANANETLLLLAHQSSKQFR 316

Query: 309 SMEENTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGA 368
            ++          L   ++ K +LRL+PIW   +++ ++  Q  TFFTKQG TM+R+I  
Sbjct: 317 FLDRAAISC---ELAEIEEAKAVLRLIPIWITSVVYTIVHAQSPTFFTKQGATMDRSISP 376

Query: 369 NFKIPPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMI 428
              +P ATLQS I +S+++ +P+YD +L+P  R FT+   GIT +QR+G G+FLS +AM+
Sbjct: 377 GLLVPAATLQSFINLSVVVFIPIYDRLLVPFARSFTQNSSGITTLQRIGTGIFLSILAMV 436

Query: 429 LAALVEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMR 488
           LAALVE KRL   +   S+   P+S++WL+PQY+I GVSD+FT+VG+QEFFY +VP  +R
Sbjct: 437 LAALVETKRLQAARDELSI---PMSVWWLIPQYVIFGVSDMFTMVGLQEFFYGQVPSELR 496

Query: 489 TTAFALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGL 540
           +   AL  S++G G++ S+ MIS+++ +T+  G+ +WF +D+ +A LD +YWLLA    +
Sbjct: 497 SVGMALNLSIYGAGNYLSSFMISVIDKITNQYGQRSWFDNDLDQAHLDYFYWLLACLGFI 520

BLAST of Carg22924 vs. ExPASy TrEMBL
Match: A0A6J1F112 (protein NRT1/ PTR FAMILY 5.8-like OS=Cucurbita moschata OX=3662 GN=LOC111438525 PE=3 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 1.9e-296
Identity = 533/541 (98.52%), Postives = 534/541 (98.71%), Query Frame = 0

Query: 3   GGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVN 62
           GGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVN
Sbjct: 5   GGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVN 64

Query: 63  TWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASSFL 122
           TWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTST LARSWSPANT SSFL
Sbjct: 65  TWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTALARSWSPANTTSSFL 124

Query: 123 FSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYF 182
           FSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPID  KTPSSSEHKAKKK LFFQWWYF
Sbjct: 125 FSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDTPKTPSSSEHKAKKKRLFFQWWYF 184

Query: 183 GVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAK 242
           GVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAK
Sbjct: 185 GVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAK 244

Query: 243 RGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEEN 302
           RGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEEN
Sbjct: 245 RGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEEN 304

Query: 303 TNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIP 362
           TNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIP
Sbjct: 305 TNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIP 364

Query: 363 PATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALV 422
           PATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALV
Sbjct: 365 PATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALV 424

Query: 423 EAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFA 482
           EAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFA
Sbjct: 425 EAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFA 484

Query: 483 LYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFDLT 542
           LYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSF L 
Sbjct: 485 LYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFVLY 544

Query: 543 I 544
           +
Sbjct: 545 V 545

BLAST of Carg22924 vs. ExPASy TrEMBL
Match: A0A6J1I187 (protein NRT1/ PTR FAMILY 5.8-like OS=Cucurbita maxima OX=3661 GN=LOC111469586 PE=3 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 1.0e-278
Identity = 506/542 (93.36%), Postives = 520/542 (95.94%), Query Frame = 0

Query: 5   GGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTW 64
           GGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLT+VMKMGNSAAAKTVNTW
Sbjct: 3   GGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTDVMKMGNSAAAKTVNTW 62

Query: 65  CGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASSFLFS 124
           CGFTSMLPLLVAPLADSYWDRYST+LASAFLYVMGLVALTSTTLA+SWSP NT SSFLFS
Sbjct: 63  CGFTSMLPLLVAPLADSYWDRYSTVLASAFLYVMGLVALTSTTLAQSWSPTNTTSSFLFS 122

Query: 125 SLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEH--KAKKKSLFFQWWYF 184
           SLYLISLGQGGYNPSLQAFGADQLDHDV ELP D AKT SSSE   K KKKSLFFQWWYF
Sbjct: 123 SLYLISLGQGGYNPSLQAFGADQLDHDVVELPTDTAKTLSSSEQKPKKKKKSLFFQWWYF 182

Query: 185 GVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAK 244
           GVCSGCLLGVTVMSYIQDT GWVIGFAIPMGAMVCSVAVFACG+KIYRYK+ EEEDGV K
Sbjct: 183 GVCSGCLLGVTVMSYIQDTLGWVIGFAIPMGAMVCSVAVFACGSKIYRYKV-EEEDGVEK 242

Query: 245 RGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFEL-QESKPLCHESSGAMKSMEE 304
           RGLVI VVELVKA+ASRL+CATNVVALSKNKPRDDVEFEL QESKPLCHESSG MKSMEE
Sbjct: 243 RGLVIKVVELVKATASRLICATNVVALSKNKPRDDVEFELQQESKPLCHESSGTMKSMEE 302

Query: 305 NTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKI 364
           N++MIPKER+CVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERN+ ANFKI
Sbjct: 303 NSDMIPKERICVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNVSANFKI 362

Query: 365 PPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAAL 424
           PPATLQSSITISIILLMPLYDN+LIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAAL
Sbjct: 363 PPATLQSSITISIILLMPLYDNILIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAAL 422

Query: 425 VEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAF 484
           VEAKRLTMTKTTSSLSY PLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAF
Sbjct: 423 VEAKRLTMTKTTSSLSYVPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAF 482

Query: 485 ALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFDL 544
           ALYNSVFGVGSFCSAIMISIVELLTSSDG  +WFSDDMSKARLDKYYWLLAFCSGLSF L
Sbjct: 483 ALYNSVFGVGSFCSAIMISIVELLTSSDGSLSWFSDDMSKARLDKYYWLLAFCSGLSFVL 542

BLAST of Carg22924 vs. ExPASy TrEMBL
Match: A0A6J1FAN2 (protein NRT1/ PTR FAMILY 5.8-like OS=Cucurbita moschata OX=3662 GN=LOC111442343 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 1.0e-241
Identity = 446/544 (81.99%), Postives = 484/544 (88.97%), Query Frame = 0

Query: 1   MSGGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKT 60
           M+ GGGGGGG   RPRLS +CILLIVVSGMERFVFKGVASN+VTYLT+VMKM NS+AAKT
Sbjct: 1   MAAGGGGGGGSPPRPRLSRSCILLIVVSGMERFVFKGVASNLVTYLTDVMKMSNSSAAKT 60

Query: 61  VNTWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASS 120
           V++WCGFTSMLPLLVAPLADSYWDRYSTILA  FLYV+GLVAL STTLAR+WSP N ASS
Sbjct: 61  VSSWCGFTSMLPLLVAPLADSYWDRYSTILAFGFLYVLGLVALASTTLARTWSPTNMASS 120

Query: 121 FLFSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWW 180
           FLF SLYLISLGQ GYN SLQAFGADQLDHD AEL   + KT SSS+ K+KKKS FFQWW
Sbjct: 121 FLFWSLYLISLGQSGYNSSLQAFGADQLDHDDAELTNTIGKTSSSSDEKSKKKSSFFQWW 180

Query: 181 YFGVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGV 240
           YFGVCSG LLGVTVMSYIQD FGWV+GFAIPM AMV SV++FACGT+IYRYK +++ED  
Sbjct: 181 YFGVCSGSLLGVTVMSYIQDNFGWVLGFAIPMCAMVSSVSLFACGTRIYRYKRDDDEDRA 240

Query: 241 AKRGLVIMVVELVKASASRLVCATNVVAL-SKNKPRDDVEFELQESKPLCHESSGAMKSM 300
            KR  V  VVE+VKA+ASRL+C  NVVA  S+N   DDVE ELQESKPLCHESSGAMK M
Sbjct: 241 EKRRFV-KVVEVVKATASRLMCCRNVVAFSSENSDDDDVELELQESKPLCHESSGAMKVM 300

Query: 301 EENTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANF 360
           E+  NMI +ERLCV DKVK++LRLLPIWT+LLMFAVIFQQPATFFTKQGMTMERNIGANF
Sbjct: 301 EDK-NMIQRERLCVPDKVKMVLRLLPIWTMLLMFAVIFQQPATFFTKQGMTMERNIGANF 360

Query: 361 KIPPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILA 420
           KIPPATLQS+ITISIILLMPLYD VLIPITRLFT  EKGITVMQRMGIGMFLSTIAMILA
Sbjct: 361 KIPPATLQSAITISIILLMPLYDKVLIPITRLFTGAEKGITVMQRMGIGMFLSTIAMILA 420

Query: 421 ALVEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTT 480
           ALVEAKRLTM KT+ S S  PLSIFWLLPQYIILG+SDIFTVVGMQEFFYSEVPVSMRTT
Sbjct: 421 ALVEAKRLTMIKTSLSSSSVPLSIFWLLPQYIILGISDIFTVVGMQEFFYSEVPVSMRTT 480

Query: 481 AFALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSF 540
           AFALYNSVFGVGSFCSAI+ISIVEL+TS +GRP+WFSD+  +ARLDKYYWLLAFCSGLSF
Sbjct: 481 AFALYNSVFGVGSFCSAILISIVELMTSMEGRPSWFSDNTREARLDKYYWLLAFCSGLSF 540

Query: 541 DLTI 544
            L +
Sbjct: 541 VLYV 542

BLAST of Carg22924 vs. ExPASy TrEMBL
Match: A0A6J1J6R9 (protein NRT1/ PTR FAMILY 5.8-like OS=Cucurbita maxima OX=3661 GN=LOC111482269 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 4.3e-240
Identity = 442/542 (81.55%), Postives = 481/542 (88.75%), Query Frame = 0

Query: 3   GGGGGGGGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVN 62
           GGGGGGGG   +PRLS +CILLIVVSGMERFVFKGVASN+VTYLT+VMKM NS+AAKTV+
Sbjct: 5   GGGGGGGGSPPQPRLSRSCILLIVVSGMERFVFKGVASNLVTYLTDVMKMSNSSAAKTVS 64

Query: 63  TWCGFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASSFL 122
           +WCGFTSMLPLLVAPLADSYWDRYSTILA  FLYV+GLVAL STTLAR+WSP N ASSFL
Sbjct: 65  SWCGFTSMLPLLVAPLADSYWDRYSTILAFGFLYVLGLVALASTTLARTWSPTNMASSFL 124

Query: 123 FSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYF 182
           F SLYLISLGQ GYN SLQAFG DQLDHD  EL   + KT SSS+ K+KKKS FFQWWYF
Sbjct: 125 FWSLYLISLGQSGYNSSLQAFGTDQLDHDDTELTTTIGKTSSSSDEKSKKKSSFFQWWYF 184

Query: 183 GVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAK 242
           GVCSG LLGVTVMSYIQD FGWV+GFAIPM AMV SV++F+CGT+IYRYK +++ED   K
Sbjct: 185 GVCSGSLLGVTVMSYIQDNFGWVLGFAIPMCAMVSSVSLFSCGTRIYRYKRDDDEDQAEK 244

Query: 243 RGLVIMVVELVKASASRLVCATNVVALS-KNKPRDDVEFELQESKPLCHESSGAMKSMEE 302
           R  V  VVE+VKA+ASRL+C  NVVA S +N   DDVE ELQESKPLCHESSGAMK M++
Sbjct: 245 RRFV-KVVEVVKATASRLMCCRNVVAFSGENSDDDDVELELQESKPLCHESSGAMKIMDD 304

Query: 303 NTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKI 362
             NMI +ERLCV DKVK++LRLLPIWT+LLMFAVIFQQPATFFTKQGMTMERNIGANFKI
Sbjct: 305 K-NMIQRERLCVPDKVKMVLRLLPIWTMLLMFAVIFQQPATFFTKQGMTMERNIGANFKI 364

Query: 363 PPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAAL 422
           PPATLQS+ITISIILLMPLYD VLIPITRLFT  EKGITVMQRMGIGMFLSTIAMILAAL
Sbjct: 365 PPATLQSAITISIILLMPLYDKVLIPITRLFTGAEKGITVMQRMGIGMFLSTIAMILAAL 424

Query: 423 VEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAF 482
           VEAKRLTMTKT SS S   LSIFWLLPQYIILG+SDIFTVVGMQEFFYSEVPVSMRTTAF
Sbjct: 425 VEAKRLTMTKTASSSSSVSLSIFWLLPQYIILGISDIFTVVGMQEFFYSEVPVSMRTTAF 484

Query: 483 ALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFDL 542
           ALYNSVFGVGSFCSAI+ISIVEL+TS +GRP+WFSD+  +ARLDKYYWLLAFCSGLSF L
Sbjct: 485 ALYNSVFGVGSFCSAILISIVELMTSMEGRPSWFSDNTREARLDKYYWLLAFCSGLSFVL 544

Query: 543 TI 544
            +
Sbjct: 545 YV 544

BLAST of Carg22924 vs. ExPASy TrEMBL
Match: A0A1S3B8W4 (protein NRT1/ PTR FAMILY 5.8-like OS=Cucumis melo OX=3656 GN=LOC103487051 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 4.9e-236
Identity = 440/537 (81.94%), Postives = 478/537 (89.01%), Query Frame = 0

Query: 12  RRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFTSML 71
           R RP LS +CILLIVVSGMERFVFKGVASN+VTYLT+VMKM NS+AAKTV++WCGFTSML
Sbjct: 14  RSRPYLSNSCILLIVVSGMERFVFKGVASNLVTYLTDVMKMSNSSAAKTVSSWCGFTSML 73

Query: 72  PLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASSFLFSSLYLISL 131
           PL+VAPLADSYWDRYSTILASAFLYV+GLVALTSTTLAR+WSP NTASSFLFSSLYLISL
Sbjct: 74  PLVVAPLADSYWDRYSTILASAFLYVLGLVALTSTTLARTWSPTNTASSFLFSSLYLISL 133

Query: 132 GQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYFGVCSGCLLG 191
           GQGGYNPSLQAFGADQLDHD AELP   AKTP  ++ K KKKSLFFQWWYFGVCSG LLG
Sbjct: 134 GQGGYNPSLQAFGADQLDHDDAELPTINAKTP--TDEKPKKKSLFFQWWYFGVCSGSLLG 193

Query: 192 VTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAKRGLVIMVVE 251
           VT+MSYIQD FGWV+GFAIPM AMV SVA+F+CGTKIYRYK + EED V KR  V  V+E
Sbjct: 194 VTIMSYIQDNFGWVLGFAIPMCAMVFSVALFSCGTKIYRYKRDVEEDEVEKRRFV-KVME 253

Query: 252 LVKASASRLVCATNVV--ALSKNKPRDDVEFELQESKPLCHESSGA-MKSM--EENTNMI 311
           + KA+ASRL+C  +VV   LS +   DDVE ELQE+KPLCHE+SGA MK+M  + NT +I
Sbjct: 254 IFKATASRLMCWRSVVTTTLSIHNSDDDVELELQETKPLCHENSGAIMKAMVDKNNTKII 313

Query: 312 PKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIPPATL 371
           P+ER+CV  KVKL+LRLLPIWT+LLMFAVIFQQPATFFTKQGMTMERNIGA+FKIPPATL
Sbjct: 314 PRERVCVPHKVKLVLRLLPIWTMLLMFAVIFQQPATFFTKQGMTMERNIGADFKIPPATL 373

Query: 372 QSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALVEAKR 431
           QS+ITISIILLMPLYD VLIPITRLFT  EKGITVMQRMGIGMFLSTIAMILAALVEAKR
Sbjct: 374 QSAITISIILLMPLYDKVLIPITRLFTGAEKGITVMQRMGIGMFLSTIAMILAALVEAKR 433

Query: 432 LTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFALYNS 491
           LTMTK   S     LSIFWLLPQYIILG+SDIFTVVGMQEFFYSEVPVSMRTT FALYNS
Sbjct: 434 LTMTKNALS-----LSIFWLLPQYIILGISDIFTVVGMQEFFYSEVPVSMRTTGFALYNS 493

Query: 492 VFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSFDLTI 544
           VFGVGSFCSAIMISIVELLTS +G+PNWFSDDM +ARLDKYYWLLAFCSGLSF L +
Sbjct: 494 VFGVGSFCSAIMISIVELLTSVEGKPNWFSDDMKEARLDKYYWLLAFCSGLSFVLYV 542

BLAST of Carg22924 vs. TAIR 10
Match: AT5G14940.1 (Major facilitator superfamily protein )

HSP 1 Score: 583.2 bits (1502), Expect = 2.4e-166
Identity = 332/542 (61.25%), Postives = 398/542 (73.43%), Query Frame = 0

Query: 9   GGLRRRPRLSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFT 68
           GG +RR  LS +C LLIV++G+ER+ FKGVASN+VTYLT+V+KM NS AA TVNTW GFT
Sbjct: 3   GGEKRR-GLSKSCALLIVIAGIERYAFKGVASNLVTYLTDVVKMSNSRAATTVNTWSGFT 62

Query: 69  SMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTAS-SFLFSSLY 128
            MLPL  AP ADSYWDR+ TILAS+ LY +GLV LT T  A S S   T S  FL++SL 
Sbjct: 63  FMLPLFSAPFADSYWDRFFTILASSSLYFVGLVGLTFTAFAGSRSTTKTISLYFLYTSLS 122

Query: 129 LISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSS--SEHKAKKKSLFFQWWYFGVC 188
           L++LG G  NPSLQAFGADQLD+D   L  D    PSS   E K+ +K+ FFQWWYFGVC
Sbjct: 123 LVALGLGVLNPSLQAFGADQLDYD---LDHDNDHEPSSENKEVKSNRKTQFFQWWYFGVC 182

Query: 189 SGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAKRGL 248
           +G LLGVTVM+YIQDTFGWVIGFAIP  +M+  + +F CG  +Y Y      D   K   
Sbjct: 183 AGSLLGVTVMAYIQDTFGWVIGFAIPTASMLLLIFLFLCGCGVYVY-----ADPDLKAKP 242

Query: 249 VIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCH----ESSGAMKSMEE 308
              ++E++K      VC  N + L  +   + +E ELQ+ KPLC+    E++   KS+ +
Sbjct: 243 FQRILEIIK----ERVCGRNKITLVNDHDLNAMELELQDQKPLCNCSNTEANTTTKSLPD 302

Query: 309 NTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKI 368
           +     K      + VKL+LRLLPIWT+LLMFAVIFQQPATFFTKQGMTM+RNIG NFKI
Sbjct: 303 DHKSC-KTGFSGLETVKLLLRLLPIWTMLLMFAVIFQQPATFFTKQGMTMKRNIGPNFKI 362

Query: 369 PPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAAL 428
           PPATLQS+IT+SIILLMP YD +LIPI +  T+ EKGI+V +RMGIGMFLS IA+++AAL
Sbjct: 363 PPATLQSTITLSIILLMPFYDKILIPIAKKLTKNEKGISVKERMGIGMFLSIIAIVIAAL 422

Query: 429 VEAKRL---TMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRT 488
           VE KRL    M KTT +L   P+SI WLLPQYI+LG+SDIFTVVGMQEFFYSEVPVSMRT
Sbjct: 423 VERKRLKISKMMKTTPNLD--PVSILWLLPQYILLGISDIFTVVGMQEFFYSEVPVSMRT 482

Query: 489 TAFALYNSVFGVGSFCSAIMISIVELLTSS-DGRPNWFSDDMSKARLDKYYWLLAFCSGL 540
             FALY SVFGVGSF SA +ISI+E  TSS  G+ NWF+DDMS+ARLD YYWLLAF S +
Sbjct: 483 MGFALYTSVFGVGSFVSAALISIIETYTSSRGGKHNWFADDMSEARLDNYYWLLAFTSAI 528

BLAST of Carg22924 vs. TAIR 10
Match: AT3G01350.1 (Major facilitator superfamily protein )

HSP 1 Score: 562.8 bits (1449), Expect = 3.3e-160
Identity = 317/537 (59.03%), Postives = 391/537 (72.81%), Query Frame = 0

Query: 17  LSGACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFTSMLPLLVA 76
           LS +C LLIV++GMER+ FKGVASN+VTYLT+V+KM NS AAKTVNTW GFTSMLPL  A
Sbjct: 10  LSKSCALLIVIAGMERYAFKGVASNLVTYLTDVVKMSNSRAAKTVNTWAGFTSMLPLFSA 69

Query: 77  PLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANTASS-FLFSSLYLISLGQGG 136
           PLAD+YWDR+ TILAS+ +Y +GLV LT T  A S S   T SS FL+SSL L+S+G G 
Sbjct: 70  PLADTYWDRFFTILASSSVYFVGLVGLTWTAFAGSRSATKTISSYFLYSSLCLVSIGLGV 129

Query: 137 YNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYFGVCSGCLLGVTVM 196
            NPSLQAFGADQLDHD+ +   D++ +    + KA +K+ FFQ WYFGVC+G L+GVTVM
Sbjct: 130 LNPSLQAFGADQLDHDL-DKNFDLS-SGDQKDAKATRKTQFFQLWYFGVCTGSLMGVTVM 189

Query: 197 SYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAKRGLVIMVVELVKA 256
           +YIQDTFGWV+GFAIP   +  S+ VF  G  IY Y        + K+       +++K 
Sbjct: 190 AYIQDTFGWVLGFAIPGIVIFLSILVFMSGCGIYVYAPGAR---LKKKTTTTPFEKILKF 249

Query: 257 SASRLVCATNVVALSKNKPRDDVEFELQESKPLC-------HESSGAMKSMEENTNMIPK 316
              R+V   ++  L+  K  D +E EL+E +PLC          S   K +E++ +   K
Sbjct: 250 IKGRVVKQRSIYTLADEKDLDAMELELEE-RPLCKCETEDIETPSTTSKGLEDDES--SK 309

Query: 317 ERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIPPATLQS 376
                 D VKL++RL PIW +LLMFAVIFQ PATFFTKQG+TM+RNIG+NFKIPPATLQS
Sbjct: 310 TVFSGIDNVKLVIRLFPIWMMLLMFAVIFQLPATFFTKQGVTMKRNIGSNFKIPPATLQS 369

Query: 377 SITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALVEAKRLT 436
           +IT+SIILLMPLYD +LIPIT+   +   GI+VM+RMG+GMFLS IA+++AA+VE KRL 
Sbjct: 370 TITLSIILLMPLYDKILIPITKRIKKNGTGISVMERMGVGMFLSIIAIVIAAIVERKRLA 429

Query: 437 MTKTTSSL-----SYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFAL 496
           +++   +L        PLSIFWLLPQYI+LG+SDIFTVVGMQEFFYSEVPV MRT  FAL
Sbjct: 430 ISQKMKTLPDYDPETVPLSIFWLLPQYILLGISDIFTVVGMQEFFYSEVPVRMRTMGFAL 489

Query: 497 YNSVFGVGSFCSAIMISIVELLTSSDG-RPNWFSDDMSKARLDKYYWLLAFCSGLSF 540
           Y SVFGVGSF SA +ISIVE  +SS G R NWF+DDMS+ARLDKYYWLLA  S +SF
Sbjct: 490 YTSVFGVGSFVSAALISIVEAYSSSTGDRQNWFADDMSEARLDKYYWLLALTSTISF 538

BLAST of Carg22924 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 380.6 bits (976), Expect = 2.3e-105
Identity = 222/526 (42.21%), Postives = 310/526 (58.94%), Query Frame = 0

Query: 24  LIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFTSMLPLLVAPLADSYW 83
           +I V   ERF + G++SN++TYLT  +    +AAA  VN W G  S+LPLL A +ADS+ 
Sbjct: 40  IIGVEVAERFAYYGISSNLITYLTGPLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFL 99

Query: 84  DRYSTILASAFLYVMGLVALT----------STTLARSWSPANTASSFLFSSLYLISLGQ 143
            R+ TILA++ LY++GL  LT           + L  S SP     +F FS+LYL++L Q
Sbjct: 100 GRFRTILAASALYIVGLGVLTLSAMIPSDCKVSNLLSSCSPRFQVITF-FSALYLVALAQ 159

Query: 144 GGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYFGVCSGCLLGVT 203
           GG+ P +QAFGADQ D    E              + K KS FF WWYFG+C G L  + 
Sbjct: 160 GGHKPCVQAFGADQFDEKEPE--------------ECKAKSSFFNWWYFGMCFGTLTTLW 219

Query: 204 VMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVAKRGLVIMVVELV 263
           V++YIQD   W +GF IP  AMV ++ V   GT  YR+ I  E+     R   + V  + 
Sbjct: 220 VLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQSPFVRIGNVYVAAVK 279

Query: 264 KASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEENTNMIPKERLCV 323
             S S L  A     L         +F       +    S ++  +EE            
Sbjct: 280 NWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNGSCSIDELEE------------ 339

Query: 324 ADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIPPATLQSSITIS 383
               K +LRL PIW   L++AV+F Q  TFFTKQG TMER+I   +KI PATLQS I++S
Sbjct: 340 ---AKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKISPATLQSFISLS 399

Query: 384 IILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALVEAKRLTMTK-- 443
           I++ +P+YD VLIPI R FT    GIT++QR+G G+FLS +AM++AALVE KRL      
Sbjct: 400 IVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAALVEMKRLKTAADY 459

Query: 444 --TTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFALYNSVFG 503
               S  +  P+S++WL+PQY++ G++D+F +VG+QEFFY +VP  +R+   ALY S+FG
Sbjct: 460 GLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELRSVGLALYLSIFG 519

Query: 504 VGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCS 536
           +G+F S+ MISI+E  TS  G+ +WF++++++A LD +YWLLA  S
Sbjct: 520 IGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLS 535

BLAST of Carg22924 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 354.8 bits (909), Expect = 1.4e-97
Identity = 211/533 (39.59%), Postives = 314/533 (58.91%), Query Frame = 0

Query: 20  ACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWCGFTSMLPLLVAPLA 79
           A + +I V   ERF + G+ SN+++YLT  +    + AA  VN W G  ++LP+L A +A
Sbjct: 35  AALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAVAAANVNAWSGIATLLPVLGAFVA 94

Query: 80  DSYWDRYSTILASAFLYVMGLVALTSTT--LARSWSPANTASSFL----FSSLYLISLGQ 139
           D++  RY TI+ S+ +YV+GL  LT +   +  +    ++ SSFL    F SLYL+++GQ
Sbjct: 95  DAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEVTSSTSSFLNVLFFFSLYLVAIGQ 154

Query: 140 GGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHKAKKKSLFFQWWYFGVCSGCLLGVT 199
            G+ P +QAFGADQ D                S+ K+ + S FF WWY  + +G    + 
Sbjct: 155 SGHKPCVQAFGADQFDE-------------KDSQEKSDRSS-FFNWWYLSLSAGICFAIL 214

Query: 200 VMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRY---KIEEEEDGVAKRGLVIMVV 259
           V+ YIQ+ F W  GF IP   MV S+ +F  G +IYRY   + EEE +   + G V  V 
Sbjct: 215 VVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRIGRVFFVA 274

Query: 260 ELVKASASRLVCATNVVALSKNKPRDDVEFELQESKPLCHESSGAMKSMEENTNMIPKER 319
              +  +S  +C    V L  N   +   F   ++  + ++SS    + + +        
Sbjct: 275 LKNQRLSSSDLCK---VELEANTSPEKQSF-FNKALLVPNDSSQGENASKSSD------- 334

Query: 320 LCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGANFKIPPATLQSSI 379
             V D   LI RL+P+W   L +A+ + Q  TFFTKQG+TM+R I    KIPPA+LQ  I
Sbjct: 335 --VEDATALI-RLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASLQVFI 394

Query: 380 TISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMILAALVEAKRLTMT 439
            ISI+L +P+YD V +PI RL T+   GIT ++R+G G+ LSTI M++AALVE KRL   
Sbjct: 395 GISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRLETA 454

Query: 440 KTTSSL----SYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMRTTAFALYNS 499
           K    +    +  P+SI+WL+PQY++LG++D++T+VGMQEFFYS+VP  +R+   ALY S
Sbjct: 455 KEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLALYLS 514

Query: 500 VFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGLSF 540
             GVGS  S+++IS+++L T  D   +WF+ ++++A LD +YWLLA  S + F
Sbjct: 515 ALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGF 539

BLAST of Carg22924 vs. TAIR 10
Match: AT1G72130.1 (Major facilitator superfamily protein )

HSP 1 Score: 353.6 bits (906), Expect = 3.1e-97
Identity = 210/542 (38.75%), Postives = 314/542 (57.93%), Query Frame = 0

Query: 9   GGLRRRPRLSG---ACILLIVVSGMERFVFKGVASNMVTYLTEVMKMGNSAAAKTVNTWC 68
           G L  R   SG   +  L+IVV   ERF + G+ASN++ YLT  +    +AAA  VN W 
Sbjct: 17  GFLEIRENTSGGWKSARLIIVVQMAERFAYFGIASNLIMYLTGPLGESTAAAAANVNAWT 76

Query: 69  GFTSMLPLLVAPLADSYWDRYSTILASAFLYVMGLVALTSTTLARSWSPANT---ASSFL 128
           G  + LPLL   LADSY  R+ TI+ S+ LY++GL  L+ +T+  S    ++     +  
Sbjct: 77  GTVAFLPLLGGFLADSYLGRFRTIIISSSLYILGLGLLSFSTMIPSHQSKDSNQLQETIF 136

Query: 129 FSSLYLISLGQGGYNPSLQAFGADQLDHDVAELPIDMAKTPSSSEHK-AKKKSLFFQWWY 188
           F SLYL+++GQGGYNP ++ FGADQ D                ++HK A+ KS FF W  
Sbjct: 137 FFSLYLVAIGQGGYNPCIKVFGADQFD---------------GNDHKEARDKSSFFNWLM 196

Query: 189 FGVCSGCLLGVTVMSYIQDTFGWVIGFAIPMGAMVCSVAVFACGTKIYRYKIEEEEDGVA 248
           FG C   L    V +YIQ+   W +GF IP  +M+ S+ +F  GT  YR+  E     V 
Sbjct: 197 FGNCISILTTRLVSTYIQENLSWSLGFGIPSVSMLLSLFLFLLGTTSYRFSTER----VG 256

Query: 249 KRGLVIMVVELVKASASRLVCATNVVALSKNKPRDDVEFELQESKP----LCHESSGAMK 308
           K+           A  SR+      +   KN+ + D++     +      L H+SS   +
Sbjct: 257 KKNPF--------ARISRV-----FMEALKNRRQPDLDIANANANETLLLLAHQSSKQFR 316

Query: 309 SMEENTNMIPKERLCVADKVKLILRLLPIWTLLLMFAVIFQQPATFFTKQGMTMERNIGA 368
            ++          L   ++ K +LRL+PIW   +++ ++  Q  TFFTKQG TM+R+I  
Sbjct: 317 FLDRAAISC---ELAEIEEAKAVLRLIPIWITSVVYTIVHAQSPTFFTKQGATMDRSISP 376

Query: 369 NFKIPPATLQSSITISIILLMPLYDNVLIPITRLFTRTEKGITVMQRMGIGMFLSTIAMI 428
              +P ATLQS I +S+++ +P+YD +L+P  R FT+   GIT +QR+G G+FLS +AM+
Sbjct: 377 GLLVPAATLQSFINLSVVVFIPIYDRLLVPFARSFTQNSSGITTLQRIGTGIFLSILAMV 436

Query: 429 LAALVEAKRLTMTKTTSSLSYAPLSIFWLLPQYIILGVSDIFTVVGMQEFFYSEVPVSMR 488
           LAALVE KRL   +   S+   P+S++WL+PQY+I GVSD+FT+VG+QEFFY +VP  +R
Sbjct: 437 LAALVETKRLQAARDELSI---PMSVWWLIPQYVIFGVSDMFTMVGLQEFFYGQVPSELR 496

Query: 489 TTAFALYNSVFGVGSFCSAIMISIVELLTSSDGRPNWFSDDMSKARLDKYYWLLAFCSGL 540
           +   AL  S++G G++ S+ MIS+++ +T+  G+ +WF +D+ +A LD +YWLLA    +
Sbjct: 497 SVGMALNLSIYGAGNYLSSFMISVIDKITNQYGQRSWFDNDLDQAHLDYFYWLLACLGFI 520

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022250.10.0e+00100.00Protein NRT1/ PTR FAMILY 5.8, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022932208.13.9e-29698.52protein NRT1/ PTR FAMILY 5.8-like [Cucurbita moschata][more]
XP_023531217.11.3e-29196.88protein NRT1/ PTR FAMILY 5.8-like [Cucurbita pepo subsp. pepo][more]
XP_022970676.12.2e-27893.36protein NRT1/ PTR FAMILY 5.8-like [Cucurbita maxima][more]
KAG6588403.11.7e-275100.00Protein NRT1/ PTR FAMILY 5.8, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9LFR13.4e-16561.25Protein NRT1/ PTR FAMILY 5.8 OS=Arabidopsis thaliana OX=3702 GN=NPF5.8 PE=2 SV=1[more]
Q9SRI24.7e-15959.03Protein NRT1/ PTR FAMILY 5.9 OS=Arabidopsis thaliana OX=3702 GN=NPF5.9 PE=2 SV=1[more]
Q0WP013.3e-10442.21Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV... [more]
Q8VZE21.9e-9639.59Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV... [more]
Q8RX674.3e-9638.75Protein NRT1/ PTR FAMILY 5.11 OS=Arabidopsis thaliana OX=3702 GN=NPF5.11 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1F1121.9e-29698.52protein NRT1/ PTR FAMILY 5.8-like OS=Cucurbita moschata OX=3662 GN=LOC111438525 ... [more]
A0A6J1I1871.0e-27893.36protein NRT1/ PTR FAMILY 5.8-like OS=Cucurbita maxima OX=3661 GN=LOC111469586 PE... [more]
A0A6J1FAN21.0e-24181.99protein NRT1/ PTR FAMILY 5.8-like OS=Cucurbita moschata OX=3662 GN=LOC111442343 ... [more]
A0A6J1J6R94.3e-24081.55protein NRT1/ PTR FAMILY 5.8-like OS=Cucurbita maxima OX=3661 GN=LOC111482269 PE... [more]
A0A1S3B8W44.9e-23681.94protein NRT1/ PTR FAMILY 5.8-like OS=Cucumis melo OX=3656 GN=LOC103487051 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G14940.12.4e-16661.25Major facilitator superfamily protein [more]
AT3G01350.13.3e-16059.03Major facilitator superfamily protein [more]
AT1G22540.12.3e-10542.21Major facilitator superfamily protein [more]
AT1G72120.11.4e-9739.59Major facilitator superfamily protein [more]
AT1G72130.13.1e-9738.75Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 10..544
e-value: 2.8E-143
score: 479.9
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 21..506
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 86..508
e-value: 3.7E-75
score: 253.2
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 16..539
NoneNo IPR availablePANTHERPTHR11654:SF82PROTEIN NRT1/ PTR FAMILY 5.8coord: 16..539

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22924-RACarg22924-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity