Carg22757 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22757
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionEukaryotic aspartyl protease family protein
LocationCarg_Chr18: 126586 .. 127875 (+)
RNA-Seq ExpressionCarg22757
SyntenyCarg22757
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAACTGCTTGTTCCGGCGAGCTCTTCTTCTTCCTCCTCCTTCTCTCTGCAGCTGCACCCTCGTGATGCTCTTCGCAATGCCGGACATACGGACTACAGGAGCTTGGTCCTCTCCCGGCTTGACCGTGACTCGTCTAGAGTTAAATCGCTTAATGATAGGCTTGAATTTGGCTTAAGTGACTTGACTAGGTCGGGTCTTCAGCCGTTGAACACGGACATTCTGCCGGAGGATCTGAGCACTCCCATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTTGGCGTTGGTCAACCGGCCAAGCCATTCTATATGGTTCTGGATACCGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCCATTTTTGACCCTACCACTTCGTCTTCTTTTACTTCCCTTCCCTGCGAATCTCCGCACTGTCAAGCTCTTGAGATGTCTGGTTGTCGTGCTGCGAAGTGTCTTTACCAGGTCTCGTACGGCGATGGTTCCTTCACCGTCGGCCAGTTCGTCACTGAAACGCTGTCGTTTGGTAACTCCGGAGTGATTAACAACGTCGCTCTCGGTTGTGGCCACGACAACGAAGGACTGTTCATCGGATCCGCCGGATTGCTTGGTCTGGGCGGCGGATCACTTTCCCTAACTTCTCAGATGAAAGCTTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCAGCATCAACTCTCGAGTTCAATTCCGCTGCACCGAATGACTCGGTGAATGCTCAATTACTCAGAAGCGGAAGAGTCAATACATTCTACTACGTTGCACTCACCGGCTTGAGCGTCGGCGGCCATCCACTCTCTATTCCGCCTTCTCTGTTTCAATTGGACGATTCCGGCCACGGCGGCATCATCGTAGATTCAGGAACCGCCATAACTAGACTGCAAACTCAGGCCTACAACTCCCTTCGCGACGCCTTCGTGAGGCTCACACCGTACTTGAAGAAGGCGAACAGCTTCGCATTATTCGACACATGTTACGATCTGTCATCGCAATCGAGAGTGACGATTCCAACAGTATCTTTCCATTTCTCGGGCGGAAAGTCGCTGCAGCTGCCGCCGAAGAACTACCTCATTCCGGTGGATTCAGCCGGAACTTTTTGTTTTGCGTTCGCCCCAACGACGTCGTCTCTGTCTATCATAGGGAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGACTTGGCTAATTCGGTGGTAGCATTTTCACCCAATAAATGTTAG

mRNA sequence

GAACTGCTTGTTCCGGCGAGCTCTTCTTCTTCCTCCTCCTTCTCTCTGCAGCTGCACCCTCGTGATGCTCTTCGCAATGCCGGACATACGGACTACAGGAGCTTGGTCCTCTCCCGGCTTGACCGTGACTCGTCTAGAGTTAAATCGCTTAATGATAGGCTTGAATTTGGCTTAAGTGACTTGACTAGGTCGGGTCTTCAGCCGTTGAACACGGACATTCTGCCGGAGGATCTGAGCACTCCCATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTTGGCGTTGGTCAACCGGCCAAGCCATTCTATATGGTTCTGGATACCGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCCATTTTTGACCCTACCACTTCGTCTTCTTTTACTTCCCTTCCCTGCGAATCTCCGCACTGTCAAGCTCTTGAGATGTCTGGTTGTCGTGCTGCGAAGTGTCTTTACCAGGTCTCGTACGGCGATGGTTCCTTCACCGTCGGCCAGTTCGTCACTGAAACGCTGTCGTTTGGTAACTCCGGAGTGATTAACAACGTCGCTCTCGGTTGTGGCCACGACAACGAAGGACTGTTCATCGGATCCGCCGGATTGCTTGGTCTGGGCGGCGGATCACTTTCCCTAACTTCTCAGATGAAAGCTTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCAGCATCAACTCTCGAGTTCAATTCCGCTGCACCGAATGACTCGGTGAATGCTCAATTACTCAGAAGCGGAAGAGTCAATACATTCTACTACGTTGCACTCACCGGCTTGAGCGTCGGCGGCCATCCACTCTCTATTCCGCCTTCTCTGTTTCAATTGGACGATTCCGGCCACGGCGGCATCATCGTAGATTCAGGAACCGCCATAACTAGACTGCAAACTCAGGCCTACAACTCCCTTCGCGACGCCTTCGTGAGGCTCACACCGTACTTGAAGAAGGCGAACAGCTTCGCATTATTCGACACATGTTACGATCTGTCATCGCAATCGAGAGTGACGATTCCAACAGTATCTTTCCATTTCTCGGGCGGAAAGTCGCTGCAGCTGCCGCCGAAGAACTACCTCATTCCGGTGGATTCAGCCGGAACTTTTTGTTTTGCGTTCGCCCCAACGACGTCGTCTCTGTCTATCATAGGGAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGACTTGGCTAATTCGGTGGTAGCATTTTCACCCAATAAATGTTAG

Coding sequence (CDS)

GAACTGCTTGTTCCGGCGAGCTCTTCTTCTTCCTCCTCCTTCTCTCTGCAGCTGCACCCTCGTGATGCTCTTCGCAATGCCGGACATACGGACTACAGGAGCTTGGTCCTCTCCCGGCTTGACCGTGACTCGTCTAGAGTTAAATCGCTTAATGATAGGCTTGAATTTGGCTTAAGTGACTTGACTAGGTCGGGTCTTCAGCCGTTGAACACGGACATTCTGCCGGAGGATCTGAGCACTCCCATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTTGGCGTTGGTCAACCGGCCAAGCCATTCTATATGGTTCTGGATACCGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCCATTTTTGACCCTACCACTTCGTCTTCTTTTACTTCCCTTCCCTGCGAATCTCCGCACTGTCAAGCTCTTGAGATGTCTGGTTGTCGTGCTGCGAAGTGTCTTTACCAGGTCTCGTACGGCGATGGTTCCTTCACCGTCGGCCAGTTCGTCACTGAAACGCTGTCGTTTGGTAACTCCGGAGTGATTAACAACGTCGCTCTCGGTTGTGGCCACGACAACGAAGGACTGTTCATCGGATCCGCCGGATTGCTTGGTCTGGGCGGCGGATCACTTTCCCTAACTTCTCAGATGAAAGCTTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCAGCATCAACTCTCGAGTTCAATTCCGCTGCACCGAATGACTCGGTGAATGCTCAATTACTCAGAAGCGGAAGAGTCAATACATTCTACTACGTTGCACTCACCGGCTTGAGCGTCGGCGGCCATCCACTCTCTATTCCGCCTTCTCTGTTTCAATTGGACGATTCCGGCCACGGCGGCATCATCGTAGATTCAGGAACCGCCATAACTAGACTGCAAACTCAGGCCTACAACTCCCTTCGCGACGCCTTCGTGAGGCTCACACCGTACTTGAAGAAGGCGAACAGCTTCGCATTATTCGACACATGTTACGATCTGTCATCGCAATCGAGAGTGACGATTCCAACAGTATCTTTCCATTTCTCGGGCGGAAAGTCGCTGCAGCTGCCGCCGAAGAACTACCTCATTCCGGTGGATTCAGCCGGAACTTTTTGTTTTGCGTTCGCCCCAACGACGTCGTCTCTGTCTATCATAGGGAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGACTTGGCTAATTCGGTGGTAGCATTTTCACCCAATAAATGTTAG

Protein sequence

ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVGQFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC
Homology
BLAST of Carg22757 vs. NCBI nr
Match: KAG7012115.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 855.1 bits (2208), Expect = 2.5e-244
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 60
           ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD
Sbjct: 1   ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 60

Query: 61  LTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 120
           LTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ
Sbjct: 61  LTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 120

Query: 121 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 180
           CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG
Sbjct: 121 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 180

Query: 181 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 240
           QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV
Sbjct: 181 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 240

Query: 241 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 300
           DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD
Sbjct: 241 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 300

Query: 301 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 360
           SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP
Sbjct: 301 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 360

Query: 361 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 420
           TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS
Sbjct: 361 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 420

Query: 421 VVAFSPNKC 430
           VVAFSPNKC
Sbjct: 421 VVAFSPNKC 429

BLAST of Carg22757 vs. NCBI nr
Match: KAG6572933.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 852.4 bits (2201), Expect = 1.6e-243
Identity = 428/429 (99.77%), Postives = 428/429 (99.77%), Query Frame = 0

Query: 1   ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 60
           ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD
Sbjct: 70  ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 129

Query: 61  LTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 120
           LTRS LQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ
Sbjct: 130 LTRSDLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 189

Query: 121 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 180
           CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG
Sbjct: 190 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 249

Query: 181 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 240
           QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV
Sbjct: 250 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 309

Query: 241 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 300
           DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD
Sbjct: 310 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 369

Query: 301 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 360
           SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP
Sbjct: 370 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 429

Query: 361 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 420
           TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS
Sbjct: 430 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 489

Query: 421 VVAFSPNKC 430
           VVAFSPNKC
Sbjct: 490 VVAFSPNKC 498

BLAST of Carg22757 vs. NCBI nr
Match: XP_022994952.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 842.4 bits (2175), Expect = 1.7e-240
Identity = 422/429 (98.37%), Postives = 425/429 (99.07%), Query Frame = 0

Query: 1   ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 60
           ELL+PA+SSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD
Sbjct: 64  ELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 123

Query: 61  LTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 120
           LTRS LQPLN DILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ
Sbjct: 124 LTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 183

Query: 121 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 180
           CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCR+AKCLYQVSYGDGSFTVG
Sbjct: 184 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVG 243

Query: 181 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 240
           QFVTETLSFGNSG INNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV
Sbjct: 244 QFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 303

Query: 241 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 300
           DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD
Sbjct: 304 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 363

Query: 301 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 360
           SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP
Sbjct: 364 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 423

Query: 361 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 420
           TVSFHFSGGKSLQL PKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS
Sbjct: 424 TVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 483

Query: 421 VVAFSPNKC 430
           VVAFSPNKC
Sbjct: 484 VVAFSPNKC 492

BLAST of Carg22757 vs. NCBI nr
Match: XP_022954867.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata])

HSP 1 Score: 839.7 bits (2168), Expect = 1.1e-239
Identity = 423/429 (98.60%), Postives = 425/429 (99.07%), Query Frame = 0

Query: 1   ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 60
           ELLVPA +SSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG +D
Sbjct: 67  ELLVPA-NSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGSTD 126

Query: 61  LTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 120
           LTRS LQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ
Sbjct: 127 LTRSDLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 186

Query: 121 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 180
           CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG
Sbjct: 187 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 246

Query: 181 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 240
           QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSL SQMKASSFSYCLV
Sbjct: 247 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLNSQMKASSFSYCLV 306

Query: 241 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 300
           DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD
Sbjct: 307 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 366

Query: 301 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 360
           SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP
Sbjct: 367 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 426

Query: 361 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 420
           TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS
Sbjct: 427 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 486

Query: 421 VVAFSPNKC 430
           VVAFSPNKC
Sbjct: 487 VVAFSPNKC 494

BLAST of Carg22757 vs. NCBI nr
Match: XP_023541582.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 839.0 bits (2166), Expect = 1.9e-239
Identity = 421/429 (98.14%), Postives = 425/429 (99.07%), Query Frame = 0

Query: 1   ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 60
           +LLVP +SSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD
Sbjct: 68  QLLVPPNSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 127

Query: 61  LTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 120
           LTRS LQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVG+PAKPFYMVLDTGSDINWLQ
Sbjct: 128 LTRSDLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGRPAKPFYMVLDTGSDINWLQ 187

Query: 121 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 180
           CQPCTDCYQQTDPIFDPTTSSSFTSL CESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG
Sbjct: 188 CQPCTDCYQQTDPIFDPTTSSSFTSLTCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 247

Query: 181 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 240
           QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV
Sbjct: 248 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 307

Query: 241 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 300
           DRDSGSASTLEFNSAAPNDSVNAQLLRSGR+NTFYYVALTGLSVGGHPLSIPPSLFQLDD
Sbjct: 308 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRLNTFYYVALTGLSVGGHPLSIPPSLFQLDD 367

Query: 301 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 360
           SGHGGIIVDSGTAITRLQTQAY SLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP
Sbjct: 368 SGHGGIIVDSGTAITRLQTQAYISLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 427

Query: 361 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 420
           TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS
Sbjct: 428 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 487

Query: 421 VVAFSPNKC 430
           VVAFSPNKC
Sbjct: 488 VVAFSPNKC 496

BLAST of Carg22757 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 8.1e-161
Identity = 276/430 (64.19%), Postives = 344/430 (80.00%), Query Frame = 0

Query: 5   PASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRS 64
           P   +SSS  SL+LH RD    + H DY+SL LSRL+RDSSRV  +  ++ F +  + RS
Sbjct: 71  PVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRS 130

Query: 65  GLQPL---NTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQC 124
            L+P+   +T    EDL+TP+VSG+SQGSGEYFSR+GVG PAK  Y+VLDTGSD+NW+QC
Sbjct: 131 DLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQC 190

Query: 125 QPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVGQ 184
           +PC DCYQQ+DP+F+PT+SS++ SL C +P C  LE S CR+ KCLYQVSYGDGSFTVG+
Sbjct: 191 EPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGE 250

Query: 185 FVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVD 244
             T+T++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+T+QMKA+SFSYCLVD
Sbjct: 251 LATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVD 310

Query: 245 RDSGSASTLEFNSA-APNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 304
           RDSG +S+L+FNS         A LLR+ +++TFYYV L+G SVGG  + +P ++F +D 
Sbjct: 311 RDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDA 370

Query: 305 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKK-ANSFALFDTCYDLSSQSRVTI 364
           SG GG+I+D GTA+TRLQTQAYNSLRDAF++LT  LKK ++S +LFDTCYD SS S V +
Sbjct: 371 SGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKV 430

Query: 365 PTVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLAN 424
           PTV+FHF+GGKSL LP KNYLIPVD +GTFCFAFAPT+SSLSIIGNVQQQGTR+ +DL+ 
Sbjct: 431 PTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSK 490

Query: 425 SVVAFSPNKC 430
           +V+  S NKC
Sbjct: 491 NVIGLSGNKC 500

BLAST of Carg22757 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.7e-110
Identity = 228/433 (52.66%), Postives = 285/433 (65.82%), Query Frame = 0

Query: 6   ASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND-RLEFGLSDLTRS 65
           + S SSSS +L L   DAL +    D   L  SRL RDS RVKS+     +    ++T +
Sbjct: 64  SDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIPGRNVTHA 123

Query: 66  GLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC 125
                     P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC PC
Sbjct: 124 P--------RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC 183

Query: 126 TDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGC--RAAKCLYQVSYGDGSFTVGQF 185
             CY Q+DPIFDP  S ++ ++PC SPHC+ L+ +GC  R   CLYQVSYGDGSFTVG F
Sbjct: 184 RRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDF 243

Query: 186 VTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSL---TSQMKASSFSYCL 245
            TETL+F  + V   VALGCGHDNEGLF+G+AGLLGLG G LS    T       FSYCL
Sbjct: 244 STETLTFRRNRV-KGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCL 303

Query: 246 VDRDSGS--ASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPL-SIPPSLF 305
           VDR + S  +S +  N+A    +    LL + +++TFYYV L G+SVGG  +  +  SLF
Sbjct: 304 VDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLF 363

Query: 306 QLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSR 365
           +LD  G+GG+I+DSGT++TRL   AY ++RDAF      LK+A  F+LFDTC+DLS+ + 
Sbjct: 364 KLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNE 423

Query: 366 VTIPTVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFD 425
           V +PTV  HF G   + LP  NYLIPVD+ G FCFAFA T   LSIIGN+QQQG RV +D
Sbjct: 424 VKVPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYD 483

Query: 426 LANSVVAFSPNKC 430
           LA+S V F+P  C
Sbjct: 484 LASSRVGFAPGGC 484

BLAST of Carg22757 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 3.4e-106
Identity = 197/427 (46.14%), Postives = 275/427 (64.40%), Query Frame = 0

Query: 7   SSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSGL 66
           S  SSS ++L+L  RD   +  + ++   + +R+ RD+ RV ++  R+   +   + S  
Sbjct: 52  SDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRY 111

Query: 67  QPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTD 126
           +         D  + IVSG  QGSGEYF R+GVG P +  YMV+D+GSD+ W+QCQPC  
Sbjct: 112 E-------VNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKL 171

Query: 127 CYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVGQFVTET 186
           CY+Q+DP+FDP  S S+T + C S  C  +E SGC +  C Y+V YGDGS+T G    ET
Sbjct: 172 CYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALET 231

Query: 187 LSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMK---ASSFSYCLVDRD 246
           L+F  + V+ NVA+GCGH N G+FIG+AGLLG+GGGS+S   Q+      +F YCLV R 
Sbjct: 232 LTFAKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRG 291

Query: 247 SGSASTLEF-NSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSG 306
           + S  +L F   A P  +    L+R+ R  +FYYV L GL VGG  + +P  +F L ++G
Sbjct: 292 TDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETG 351

Query: 307 HGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTV 366
            GG+++D+GTA+TRL T AY + RD F   T  L +A+  ++FDTCYDLS    V +PTV
Sbjct: 352 DGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTV 411

Query: 367 SFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVV 426
           SF+F+ G  L LP +N+L+PVD +GT+CFAFA + + LSIIGN+QQ+G +V+FD AN  V
Sbjct: 412 SFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFV 470

Query: 427 AFSPNKC 430
            F PN C
Sbjct: 472 GFGPNVC 470

BLAST of Carg22757 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.6e-74
Identity = 157/347 (45.24%), Postives = 205/347 (59.08%), Query Frame = 0

Query: 89  GSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPC 148
           G GEY   + +G PA+PF  ++DTGSD+ W QCQPCT C+ Q+ PIF+P  SSSF++LPC
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPC 150

Query: 149 ESPHCQALEMSGCRAAKCLYQVSYGDGSFTVGQFVTETLSFGNSGVINNVALGCGHDNEG 208
            S  CQAL    C    C Y   YGDGS T G   TETL+FG S  I N+  GCG +N+G
Sbjct: 151 SSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQG 210

Query: 209 LFIGS-AGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSASTLEFNSAAPN---DSVNAQ 268
              G+ AGL+G+G G LSL SQ+  + FSYC+    S + S L   S A +    S N  
Sbjct: 211 FGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTT 270

Query: 269 LLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLD-DSGHGGIIVDSGTAITRLQTQAYN 328
           L++S ++ TFYY+ L GLSVG   L I PS F L+ ++G GGII+DSGT +T     AY 
Sbjct: 271 LIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQ 330

Query: 329 SLRDAFVRLTPYLKKANSFALFDTCYDL-SSQSRVTIPTVSFHFSGGKSLQLPPKNYLIP 388
           S+R  F+          S + FD C+   S  S + IPT   HF GG  L+LP +NY I 
Sbjct: 331 SVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFIS 390

Query: 389 VDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 430
             S G  C A   ++  +SI GN+QQQ   V +D  NSVV+F+  +C
Sbjct: 391 -PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Carg22757 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 4.4e-74
Identity = 165/401 (41.15%), Postives = 220/401 (54.86%), Query Frame = 0

Query: 35  LVLSRLDRDSSRVKSLNDRLEFGLSDLTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYF 94
           L+   + R   R++S+N  L+      + SG++            TP+ +    G GEY 
Sbjct: 60  LIKRAIKRGERRMRSINAMLQ------SSSGIE------------TPVYA----GDGEYL 119

Query: 95  SRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQ 154
             V +G P   F  ++DTGSD+ W QC+PCT C+ Q  PIF+P  SSSF++LPCES +CQ
Sbjct: 120 MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQ 179

Query: 155 ALEMSGCRAAKCLYQVSYGDGSFTVGQFVTETLSFGNSGVINNVALGCGHDNEGLFIGS- 214
            L    C   +C Y   YGDGS T G   TET +F  S V  N+A GCG DN+G   G+ 
Sbjct: 180 DLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSV-PNIAFGCGEDNQGFGQGNG 239

Query: 215 AGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSASTLEFNSAA---PNDSVNAQLLRSGR 274
           AGL+G+G G LSL SQ+    FSYC+    S S STL   SAA   P  S +  L+ S  
Sbjct: 240 AGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSL 299

Query: 275 VNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFV 334
             T+YY+ L G++VGG  L IP S FQL D G GG+I+DSGT +T L   AYN++  AF 
Sbjct: 300 NPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFT 359

Query: 335 RLTPYLKKANSFALFDTCYDLSSQ-SRVTIPTVSFHFSGGKSLQLPPKNYLIPVDSAGTF 394
                     S +   TC+   S  S V +P +S  F GG  L L  +N LI   + G  
Sbjct: 360 DQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILIS-PAEGVI 419

Query: 395 CFAFAPTTS-SLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 430
           C A   ++   +SI GN+QQQ T+V +DL N  V+F P +C
Sbjct: 420 CLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of Carg22757 vs. ExPASy TrEMBL
Match: A0A6J1JXD0 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111490538 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 8.2e-241
Identity = 422/429 (98.37%), Postives = 425/429 (99.07%), Query Frame = 0

Query: 1   ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 60
           ELL+PA+SSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD
Sbjct: 64  ELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 123

Query: 61  LTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 120
           LTRS LQPLN DILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ
Sbjct: 124 LTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 183

Query: 121 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 180
           CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCR+AKCLYQVSYGDGSFTVG
Sbjct: 184 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVG 243

Query: 181 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 240
           QFVTETLSFGNSG INNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV
Sbjct: 244 QFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 303

Query: 241 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 300
           DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD
Sbjct: 304 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 363

Query: 301 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 360
           SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP
Sbjct: 364 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 423

Query: 361 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 420
           TVSFHFSGGKSLQL PKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS
Sbjct: 424 TVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 483

Query: 421 VVAFSPNKC 430
           VVAFSPNKC
Sbjct: 484 VVAFSPNKC 492

BLAST of Carg22757 vs. ExPASy TrEMBL
Match: A0A6J1GS20 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456998 PE=3 SV=1)

HSP 1 Score: 839.7 bits (2168), Expect = 5.3e-240
Identity = 423/429 (98.60%), Postives = 425/429 (99.07%), Query Frame = 0

Query: 1   ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 60
           ELLVPA +SSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG +D
Sbjct: 67  ELLVPA-NSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGSTD 126

Query: 61  LTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 120
           LTRS LQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ
Sbjct: 127 LTRSDLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 186

Query: 121 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 180
           CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG
Sbjct: 187 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 246

Query: 181 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 240
           QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSL SQMKASSFSYCLV
Sbjct: 247 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLNSQMKASSFSYCLV 306

Query: 241 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 300
           DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD
Sbjct: 307 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 366

Query: 301 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 360
           SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP
Sbjct: 367 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 426

Query: 361 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 420
           TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS
Sbjct: 427 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 486

Query: 421 VVAFSPNKC 430
           VVAFSPNKC
Sbjct: 487 VVAFSPNKC 494

BLAST of Carg22757 vs. ExPASy TrEMBL
Match: A0A6J1C6K9 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN=LOC111008878 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.3e-221
Identity = 389/429 (90.68%), Postives = 405/429 (94.41%), Query Frame = 0

Query: 1   ELLVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSD 60
           E LVPA   +SSSFSLQLHPRDALRNAGH DY+SLVLSRLDRDSSRVKSLNDRL+F LSD
Sbjct: 61  EQLVPA---NSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSD 120

Query: 61  LTRSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 120
           L RS LQPL T+ILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ
Sbjct: 121 LKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 180

Query: 121 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVG 180
           CQPCTDCYQQ+DPIFDP TSSSF SLPCES  CQALEMSGCRAAKCLYQVSYGDGSFTVG
Sbjct: 181 CQPCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVG 240

Query: 181 QFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 240
           +FVTETLSFGNSG INNVALGCGHDNEGLF+GSAGLLGLGGGSLSLTSQMKASSFSYCLV
Sbjct: 241 EFVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLV 300

Query: 241 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 300
           DRDSGS+S+L+FNS AP+DSV A LLRSGRV+TFYYV LTGLSVGG  LSIPPSLFQ+DD
Sbjct: 301 DRDSGSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDD 360

Query: 301 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 360
           SG+GGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYL+K N FALFDTCYDLSSQSRVTIP
Sbjct: 361 SGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIP 420

Query: 361 TVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 420
           TVSFHFSGGKSL LPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS
Sbjct: 421 TVSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 480

Query: 421 VVAFSPNKC 430
           +V FSPNKC
Sbjct: 481 LVGFSPNKC 486

BLAST of Carg22757 vs. ExPASy TrEMBL
Match: A0A6J1E815 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111431581 PE=3 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 1.9e-213
Identity = 371/421 (88.12%), Postives = 395/421 (93.82%), Query Frame = 0

Query: 9   SSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSGLQP 68
           +SSSSFSLQLH RDAL+NAGH DY+SLVLSRLDRDSSRVKSLNDRLEF LSDL RS LQP
Sbjct: 74  NSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKRSDLQP 133

Query: 69  LNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCY 128
           L T+ILPEDLSTPIVSG+SQGSGEYFSR+GVG+PAKPFYMVLDTGSDINWLQCQPCTDCY
Sbjct: 134 LQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDINWLQCQPCTDCY 193

Query: 129 QQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVGQFVTETLS 188
           QQTDPIFDP TSSSF SLPCESP CQALE SGCRAAKCLYQV+YGDGSFTVG+FVTE+LS
Sbjct: 194 QQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEFVTESLS 253

Query: 189 FGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSAS 248
           FGNSG INNVALGCGHDNEGLF+GSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS S+S
Sbjct: 254 FGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSSSSS 313

Query: 249 TLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIV 308
            LEFNSAAP+DSVNA LLRSGRV+TFYYV L GLSVGG  LSIPPSLFQ+DD+G+GGIIV
Sbjct: 314 ALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDAGYGGIIV 373

Query: 309 DSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFSG 368
           DSGTAITRLQTQAYN+LRDAFV  TPYL++ N FALFDTCYDLSSQSRVTIPT+SF F+G
Sbjct: 374 DSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQSRVTIPTLSFQFAG 433

Query: 369 GKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNK 428
           GKSLQLPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRV+FDLANS+V FSPNK
Sbjct: 434 GKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLVGFSPNK 493

Query: 429 C 430
           C
Sbjct: 494 C 494

BLAST of Carg22757 vs. ExPASy TrEMBL
Match: A0A6J1KJJ8 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495193 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 4.0e-211
Identity = 370/427 (86.65%), Postives = 396/427 (92.74%), Query Frame = 0

Query: 3   LVPASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLT 62
           +VPA  +SSSSFSLQLH RDAL+NAGH DY+SLVLSRLDRDSSRVKSLNDRLEF LSDL 
Sbjct: 71  VVPA--NSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLK 130

Query: 63  RSGLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQ 122
           RS LQPL T+ILPEDLSTPIVSG+SQGSGEYFSR+GVGQPAKPFYMVLDTGSDINWLQCQ
Sbjct: 131 RSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQCQ 190

Query: 123 PCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVGQF 182
           PCTDCYQQTDPIFDP TSSSF SL CESP CQALE SGCRAAKCLYQV+YGDGSFTVG+F
Sbjct: 191 PCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEF 250

Query: 183 VTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDR 242
           VTE+LSFGNSG INNVALGCGHDNEGLF+GSAGLLGLGGGSLSL SQMKASSFSYCLVDR
Sbjct: 251 VTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASSFSYCLVDR 310

Query: 243 DSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSG 302
           DS S+S LEFNSAAP+D+VNA LLRSGRV+TFYYV L GLSVGG  LSIPPSLFQ+DD+G
Sbjct: 311 DSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDAG 370

Query: 303 HGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTV 362
           +GGIIVDSGTAITRLQTQAYN+LRDAFV  TPYL++ N  ALFDTCYDLSSQSRVTIPT+
Sbjct: 371 YGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQSRVTIPTL 430

Query: 363 SFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVV 422
           SF F+GGKSLQLPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRV+FDLANS++
Sbjct: 431 SFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLL 490

Query: 423 AFSPNKC 430
            FSPNKC
Sbjct: 491 GFSPNKC 495

BLAST of Carg22757 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 568.2 bits (1463), Expect = 5.8e-162
Identity = 276/430 (64.19%), Postives = 344/430 (80.00%), Query Frame = 0

Query: 5   PASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRS 64
           P   +SSS  SL+LH RD    + H DY+SL LSRL+RDSSRV  +  ++ F +  + RS
Sbjct: 71  PVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRS 130

Query: 65  GLQPL---NTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQC 124
            L+P+   +T    EDL+TP+VSG+SQGSGEYFSR+GVG PAK  Y+VLDTGSD+NW+QC
Sbjct: 131 DLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQC 190

Query: 125 QPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVGQ 184
           +PC DCYQQ+DP+F+PT+SS++ SL C +P C  LE S CR+ KCLYQVSYGDGSFTVG+
Sbjct: 191 EPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGE 250

Query: 185 FVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVD 244
             T+T++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+T+QMKA+SFSYCLVD
Sbjct: 251 LATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVD 310

Query: 245 RDSGSASTLEFNSA-APNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 304
           RDSG +S+L+FNS         A LLR+ +++TFYYV L+G SVGG  + +P ++F +D 
Sbjct: 311 RDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDA 370

Query: 305 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKK-ANSFALFDTCYDLSSQSRVTI 364
           SG GG+I+D GTA+TRLQTQAYNSLRDAF++LT  LKK ++S +LFDTCYD SS S V +
Sbjct: 371 SGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKV 430

Query: 365 PTVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLAN 424
           PTV+FHF+GGKSL LP KNYLIPVD +GTFCFAFAPT+SSLSIIGNVQQQGTR+ +DL+ 
Sbjct: 431 PTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSK 490

Query: 425 SVVAFSPNKC 430
           +V+  S NKC
Sbjct: 491 NVIGLSGNKC 500

BLAST of Carg22757 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 555.4 bits (1430), Expect = 3.9e-158
Identity = 266/423 (62.88%), Postives = 346/423 (81.80%), Query Frame = 0

Query: 9   SSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSGLQP 68
           S+SSSFSLQLH R ++R   H+DY+SL L+RL+RD++RVKSL  RL+  +++++++ L+P
Sbjct: 62  SASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKP 121

Query: 69  LNTDILPE--DLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTD 128
           ++T    E  D+  P++SG++QGSGEYF+RVG+G+PA+  YMVLDTGSD+NWLQC PC D
Sbjct: 122 ISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCAD 181

Query: 129 CYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVGQFVTET 188
           CY QT+PIF+P++SSS+  L C++P C ALE+S CR A CLY+VSYGDGS+TVG F TET
Sbjct: 182 CYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATET 241

Query: 189 LSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGS 248
           L+ G S ++ NVA+GCGH NEGLF+G+AGLLGLGGG L+L SQ+  +SFSYCLVDRDS S
Sbjct: 242 LTIG-STLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDS 301

Query: 249 ASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGI 308
           AST++F ++   D+V A LLR+ +++TFYY+ LTG+SVGG  L IP S F++D+SG GGI
Sbjct: 302 ASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGI 361

Query: 309 IVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHF 368
           I+DSGTA+TRLQT+ YNSLRD+FV+ T  L+KA   A+FDTCY+LS+++ V +PTV+FHF
Sbjct: 362 IIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHF 421

Query: 369 SGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSP 428
            GGK L LP KNY+IPVDS GTFC AFAPT SSL+IIGNVQQQGTRV FDLANS++ FS 
Sbjct: 422 PGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSS 481

Query: 429 NKC 430
           NKC
Sbjct: 482 NKC 483

BLAST of Carg22757 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 401.0 bits (1029), Expect = 1.2e-111
Identity = 228/433 (52.66%), Postives = 285/433 (65.82%), Query Frame = 0

Query: 6   ASSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND-RLEFGLSDLTRS 65
           + S SSSS +L L   DAL +    D   L  SRL RDS RVKS+     +    ++T +
Sbjct: 64  SDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIPGRNVTHA 123

Query: 66  GLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC 125
                     P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC PC
Sbjct: 124 P--------RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC 183

Query: 126 TDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGC--RAAKCLYQVSYGDGSFTVGQF 185
             CY Q+DPIFDP  S ++ ++PC SPHC+ L+ +GC  R   CLYQVSYGDGSFTVG F
Sbjct: 184 RRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDF 243

Query: 186 VTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSL---TSQMKASSFSYCL 245
            TETL+F  + V   VALGCGHDNEGLF+G+AGLLGLG G LS    T       FSYCL
Sbjct: 244 STETLTFRRNRV-KGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCL 303

Query: 246 VDRDSGS--ASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPL-SIPPSLF 305
           VDR + S  +S +  N+A    +    LL + +++TFYYV L G+SVGG  +  +  SLF
Sbjct: 304 VDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLF 363

Query: 306 QLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSR 365
           +LD  G+GG+I+DSGT++TRL   AY ++RDAF      LK+A  F+LFDTC+DLS+ + 
Sbjct: 364 KLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNE 423

Query: 366 VTIPTVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFD 425
           V +PTV  HF G   + LP  NYLIPVD+ G FCFAFA T   LSIIGN+QQQG RV +D
Sbjct: 424 VKVPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYD 483

Query: 426 LANSVVAFSPNKC 430
           LA+S V F+P  C
Sbjct: 484 LASSRVGFAPGGC 484

BLAST of Carg22757 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 390.2 bits (1001), Expect = 2.2e-108
Identity = 226/436 (51.83%), Postives = 282/436 (64.68%), Query Frame = 0

Query: 8   SSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSGLQ 67
           S S++S S+ L   DAL +        L   RL RDS RVKS+       L+ ++     
Sbjct: 55  SESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSIT-----SLAAVSTGRNA 114

Query: 68  PLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDC 127
              T       S  ++SG SQGSGEYF R+GVG PA   YMVLDTGSD+ WLQC PC  C
Sbjct: 115 TKRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKAC 174

Query: 128 YQQTDPIFDPTTSSSFTSLPCESPHCQAL-EMSGC---RAAKCLYQVSYGDGSFTVGQFV 187
           Y QTD IFDP  S +F ++PC S  C+ L + S C   R+  CLYQVSYGDGSFT G F 
Sbjct: 175 YNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFS 234

Query: 188 TETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMK---ASSFSYCLV 247
           TETL+F +   +++V LGCGHDNEGLF+G+AGLLGLG G LS  SQ K      FSYCLV
Sbjct: 235 TETLTF-HGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLV 294

Query: 248 DR-DSGSA----STLEF-NSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPL-SIPP 307
           DR  SGS+    ST+ F N+A P  SV   LL + +++TFYY+ L G+SVGG  +  +  
Sbjct: 295 DRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSE 354

Query: 308 SLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSS 367
           S F+LD +G+GG+I+DSGT++TRL   AY +LRDAF      LK+A S++LFDTC+DLS 
Sbjct: 355 SQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSG 414

Query: 368 QSRVTIPTVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRV 427
            + V +PTV FHF GG+ + LP  NYLIPV++ G FCFAFA T  SLSIIGN+QQQG RV
Sbjct: 415 MTTVKVPTVVFHFGGGE-VSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRV 474

Query: 428 NFDLANSVVAFSPNKC 430
            +DL  S V F    C
Sbjct: 475 AYDLVGSRVGFLSRAC 483

BLAST of Carg22757 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 386.7 bits (992), Expect = 2.4e-107
Identity = 197/427 (46.14%), Postives = 275/427 (64.40%), Query Frame = 0

Query: 7   SSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSGL 66
           S  SSS ++L+L  RD   +  + ++   + +R+ RD+ RV ++  R+   +   + S  
Sbjct: 52  SDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRY 111

Query: 67  QPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTD 126
           +         D  + IVSG  QGSGEYF R+GVG P +  YMV+D+GSD+ W+QCQPC  
Sbjct: 112 E-------VNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKL 171

Query: 127 CYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKCLYQVSYGDGSFTVGQFVTET 186
           CY+Q+DP+FDP  S S+T + C S  C  +E SGC +  C Y+V YGDGS+T G    ET
Sbjct: 172 CYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALET 231

Query: 187 LSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMK---ASSFSYCLVDRD 246
           L+F  + V+ NVA+GCGH N G+FIG+AGLLG+GGGS+S   Q+      +F YCLV R 
Sbjct: 232 LTFAKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRG 291

Query: 247 SGSASTLEF-NSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSG 306
           + S  +L F   A P  +    L+R+ R  +FYYV L GL VGG  + +P  +F L ++G
Sbjct: 292 TDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETG 351

Query: 307 HGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTV 366
            GG+++D+GTA+TRL T AY + RD F   T  L +A+  ++FDTCYDLS    V +PTV
Sbjct: 352 DGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTV 411

Query: 367 SFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVV 426
           SF+F+ G  L LP +N+L+PVD +GT+CFAFA + + LSIIGN+QQ+G +V+FD AN  V
Sbjct: 412 SFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFV 470

Query: 427 AFSPNKC 430
            F PN C
Sbjct: 472 GFGPNVC 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012115.12.5e-244100.00Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp... [more]
KAG6572933.11.6e-24399.77Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp... [more]
XP_022994952.11.7e-24098.37protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima][more]
XP_022954867.11.1e-23998.60protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata][more]
XP_023541582.11.9e-23998.14protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LS408.1e-16164.19Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ31.7e-11052.66Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q9LHE33.4e-10646.14Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q766C32.6e-7445.24Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C24.4e-7441.15Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1JXD08.2e-24198.37protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GS205.3e-24098.60protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1C6K93.3e-22190.68protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1E8151.9e-21388.12protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KJJ84.0e-21186.65protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT3G18490.15.8e-16264.19Eukaryotic aspartyl protease family protein [more]
AT1G25510.13.9e-15862.88Eukaryotic aspartyl protease family protein [more]
AT1G01300.11.2e-11152.66Eukaryotic aspartyl protease family protein [more]
AT3G61820.12.2e-10851.83Eukaryotic aspartyl protease family protein [more]
AT3G20015.12.4e-10746.14Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 274..425
e-value: 3.1E-38
score: 131.1
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 70..247
e-value: 7.4E-50
score: 171.6
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 248..429
e-value: 3.3E-52
score: 178.9
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 87..429
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 93..252
e-value: 2.5E-47
score: 161.4
NoneNo IPR availablePANTHERPTHR13683:SF274PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1coord: 7..429
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 7..429
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 108..119
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 93..425
score: 45.691963

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22757-RACarg22757-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity