Carg22727 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22727
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionWRKY transcription factor SUSIBA2-like
LocationCarg_Chr11: 5035303 .. 5038951 (+)
RNA-Seq ExpressionCarg22727
SyntenyCarg22727
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGTTCATGGAGGTCTATGGCGAGCACAGAGCCTCTAAATTTGGTGATGATGATGTTCATGTGGATGTCAATGGTGGCAGCTTGGCTGATAGACGAGCTTCCAAGAGAGGGAGAAATGGGTTCAATATCGCTATGGCTCGGTGCCCGTTGGGTTCGTCGATGGTTCGATCTCCTTTCCTCACAATTCCTTCCGGGTTTAGCCCGACCGTGTTGCTTGATTCGCCGATTATGTTGCTCAATACCCATGTGAGATTTCTTCTTTCTTCTTGTTTTCTTGTGATTTGAACGTCTGATCTTGAGGAATGATCAGATCTGGTGAACGAACGAGCTAATCGGGAATATTTTGTTATTGAAATCGTATGGATCTCTTGTTGGGTTGTTTTAATTTTACGTGGAAATCTTAACCGTAGAGGTATTTGATCTCGTTCTAGATTAGGATGATGTTTGGAGGATGAATTCTTCGAACTAGCTAGGGTTAATAAGAGCTAATGTAAATGATAAAAGGTTTCGAAGAAATGGATTCGGTTGGTTTCTGAACTAATAGATTTGATTGGATTGGATTGGATCGTGTTATGTCATGTCAGGATATTCCATCTCCAACAACGGGGACGTTTTCCGATACATTCCATAAAGGATGAGAATTCGTTGCTGAATCCTATCATGCTTGAAGATGGGAGTAGCTATGGCAGTGAGGATTCGTACTACAGGTTCACGCCTCGTGGGGAGCTCCGGCTTGAAAATCAGGTAATTGATTAACGCTATAATTTTTTCGAATATGTGAACGAGAAGGCATTGCTTCTCGAGTAAGATATGACACTCGAGTAGTACAAGAATAATGCATGAATGAAATAAGACCATATCTAAATGAGAAACATCTCGAAGATATACAAATAGGGTAAAGATTGCCTTAAAAACGATAGTTATACTAACAGAGTGTAGTTTCTTCTTCGGTTGCTGGTTTAGAACAATTCTATGTTGGATGATCTCGAAAAAATTTTGTGCGGACAACCAAGACAACTAAATAACATGATCGAGATGCTAATGATTGTTAGGATCATTAGACATTGAATTCTAATTCAAATCCCGAGTTGAGTATTTCAATGAATAATAATATTAAAAAAATTAAAGCGAAACATAAAATCTTATACGAGCCAAAACCTTTTCAAGGCCTTCAACCCTTTAAAACTCGGTTTATAGATTATATAGTCATGATTTTTCGTTATATATAGATTTTTTTTTTTTTTGGAATATTTATCTAAAAAATATTTGATTAGTTTCAAAATAAAATATTATATTTGTGATTAGGATACATATTTATGTTATTTATTATAATAATTTTATTTTATTAAGTTTGGGGGAAGGAATTATTAATTCCAAACTATTTTTTTTTTAATTTAATTTATTTATTATTATTTTTTATATATATAATAAATTCTTAACTAGGGACTCCGTGAATAGGAATCTATTGTAATGACTCTAACTAACTCTTAAAACACGAACGTCCTAGGTTTATCTCATCGTATAAGATGTCCTTATTCTCGTCTTTGTCTGGTTTTTTTCTTAGAAATATTGAGTATTAAAAATACTCGAGTAACTAACAATAATGATAATGATTTATGATTCGAACTTTTGGTTGTTTAGGAGAAAGATATTGACTCTATGATATAGTTTGACCCAGATTAACGTACCAACTTTTCAAATAGAATTATAAAAGCTCGTATAGTAACGACTTTGCCTCTTAAAACACGACTTACCCATGATTAAAAACCTTTAAATCTATCTCAAAATCAAACTCGGGTTTATCTCATGCAAGGTCAACCGTCTAAGATGCTCTTGTTCTCGTTCTTGTCTGAATTTTTTTTTTTTTTAGAAATATTGAGTATTAAAAATACCTGAGTAGCTAACCAGAATGATTCATGATTCGAACTTTTACTCGTTTAGGAAGGTGAAACGGATCATCAGGGGGTCGAATCAGAGAAAACACTGATGGATTATGAATTACTAGCTGAATTTCCCAAAGAGGGTTGTTCTGTATCAGAACAATATGAATTGGGTTCATCCTCCACTGATAAGAACAATGTGGAAACCTGTCTTTCCTCTGTAACTCATAATCAAGACATTGAGCAGCCGGCGGCGCCGCCGCCGCCGCCGTTAGAAACAGAACACAAAGGGTCTTATGTTCCTGTCGGAATGCTAAAAACATCAGAAGATGGATACAACTGGAGAAAGTACGGCCAGAAACAGGTGAAGGGCAGCGAATATCCAAGAAGCTATTACAAATGCACGCATCCAAATTGTATAGTAAAGAAGAAGGTGGAGCGATCGCTTGACGGTCAGATCACAGAGATTATATATACCGGCGCTCATAATCATTCTAAGCCGGACCCCACTCGCCGGGCTGTGCTTGGATCAGTGCTTGCTCCAGATGATGCTCCGGACACCGGCGAAGGCGGTGGAAGCCGCGCCAAACTTGAAGGTGCACTGCCATGGCGGAACAGTCACTATGGGGTGAAGGATTGGAGCGTTGATGGGCTAGAAAGGACATCCTCTGTTTCAGCTGTGACAGAGCTTTCTGAGCCATTGTATGGGAAAACTGTTGGTGGGTTTGAATCCATTGAAACTCCCGAGCTTTCCTCCACTCTTGCTAGCCATGATGATGACGACGGCGGCGGCGGCGGAGGAAGCGGCGGCGGTGGAAACGATGATGATGAACTCACAAATCAGGGGAGTATTTCAGCTGATGATGCAGAGCCTGAGCTGAAAAGAAGGTAAAAAACAACAAAAAAAACATCATGGGGTTTGTTAAAAACGTGTTTTTGTTCATTAATTACAAGGTTTAATCATTGTTTCAGACGGAAAGAGGGGATTTCCATGGAGACAAGCTTGGGTTCGCGCTCTGTTCGTGAACAGAGAGTAGTAGTTCAAATCGAAACAGAGTTCGACATACTTGAAGATGGGTATCGATGGAGGAAATATGGCCAAAAAGTTGTGAAAGGAAACCCAAATCCAAGGTAAAAAAACATTCAAATCCAAGCTAGAAATCGTTTCTATTGCATTTTATGAAATGGGTTTGTTCTTCAGGAGCTATTACAAGTGCACGAGTGCAGGGTGTTCAGTAAGAAAACATGTCGAGAGAGCATCGCATGATTTGAAATGTGTAATAACAACGTACGAAGGAAAGCACAACCATTTAGTGCCAGCAGCGAGGAACAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCACCGCGCAAGCCTCCTCTGCTGCTGCTGCTACTCAGGCGGCGGCGGCGCCGACAAGAAAGTCAGAAACACAAATCCAAGATCTTTCCACACGGTTTTATCCGACACCTGAATTCAGCAGCCATGAGTATCAGCGGTCGTTTGGAACAGCGCCGCCATTTTGTCAGCTGAAGTTCCCTCCATTGCAGACGGCCTTCCCTGCTTTGGACTTCCCCATTTCATTGCCATTGAGCCAAAATCTGTCGGCTTTTCAGGTGTTCTTGGCTGAGAGATTCCTCACGCCAAAACAGGAGCATGAGGAGGATAATATCTATGCTTCTTTTCAGGCAATGACTGATACTTCAAGTGGATCTTCTTCTTCCTCTGTTTCCTCTGTTTATCAGCAAGTTATGGGGAAATTTCCATAG

mRNA sequence

ATGCAGTTCATGGAGGTCTATGGCGAGCACAGAGCCTCTAAATTTGGTGATGATGATGTTCATGTGGATGTCAATGGTGGCAGCTTGGCTGATAGACGAGCTTCCAAGAGAGGGAGAAATGGGTTCAATATCGCTATGGCTCGGTGCCCGTTGGGTTCGTCGATGGTTCGATCTCCTTTCCTCACAATTCCTTCCGGGTTTAGCCCGACCGTGTTGCTTGATTCGCCGATTATGATATTCCATCTCCAACAACGGGGACGTTTTCCGATACATTCCATAAAGGATGAGAATTCGTTGCTGAATCCTATCATGCTTGAAGATGGGAGTAGCTATGGCAGTGAGGATTCGTACTACAGGTTCACGCCTCGTGGGGAGCTCCGGCTTGAAAATCAGGAAGGTGAAACGGATCATCAGGGGGTCGAATCAGAGAAAACACTGATGGATTATGAATTACTAGCTGAATTTCCCAAAGAGGGTTGTTCTGTATCAGAACAATATGAATTGGGTTCATCCTCCACTGATAAGAACAATGTGGAAACCTGTCTTTCCTCTGTAACTCATAATCAAGACATTGAGCAGCCGGCGGCGCCGCCGCCGCCGCCGTTAGAAACAGAACACAAAGGGTCTTATGTTCCTGTCGGAATGCTAAAAACATCAGAAGATGGATACAACTGGAGAAAGTACGGCCAGAAACAGGTGAAGGGCAGCGAATATCCAAGAAGCTATTACAAATGCACGCATCCAAATTGTATAGTAAAGAAGAAGGTGGAGCGATCGCTTGACGGTCAGATCACAGAGATTATATATACCGGCGCTCATAATCATTCTAAGCCGGACCCCACTCGCCGGGCTGTGCTTGGATCAGTGCTTGCTCCAGATGATGCTCCGGACACCGGCGAAGGCGGTGGAAGCCGCGCCAAACTTGAAGGTGCACTGCCATGGCGGAACAGTCACTATGGGGTGAAGGATTGGAGCGTTGATGGGCTAGAAAGGACATCCTCTGTTTCAGCTGTGACAGAGCTTTCTGAGCCATTGTATGGGAAAACTGTTGGTGGGTTTGAATCCATTGAAACTCCCGAGCTTTCCTCCACTCTTGCTAGCCATGATGATGACGACGGCGGCGGCGGCGGAGGAAGCGGCGGCGGTGGAAACGATGATGATGAACTCACAAATCAGGGGAGTATTTCAGCTGATGATGCAGAGCCTGAGCTGAAAAGAAGACGGAAAGAGGGGATTTCCATGGAGACAAGCTTGGGTTCGCGCTCTGTTCGTGAACAGAGAGTAGTAGTTCAAATCGAAACAGAGTTCGACATACTTGAAGATGGGTATCGATGGAGGAAATATGGCCAAAAAGTTGTGAAAGGAAACCCAAATCCAAGGAGCTATTACAAGTGCACGAGTGCAGGGTGTTCAGTAAGAAAACATGTCGAGAGAGCATCGCATGATTTGAAATGTGTAATAACAACGTACGAAGGAAAGCACAACCATTTAGTGCCAGCAGCGAGGAACAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCACCGCGCAAGCCTCCTCTGCTGCTGCTGCTACTCAGGCGGCGGCGGCGCCGACAAGAAAGTCAGAAACACAAATCCAAGATCTTTCCACACGGTTTTATCCGACACCTGAATTCAGCAGCCATGAGTATCAGCGGTCGTTTGGAACAGCGCCGCCATTTTGTCAGCTGAAGTTCCCTCCATTGCAGACGGCCTTCCCTGCTTTGGACTTCCCCATTTCATTGCCATTGAGCCAAAATCTGTCGGCTTTTCAGGTGTTCTTGGCTGAGAGATTCCTCACGCCAAAACAGGAGCATGAGGAGGATAATATCTATGCTTCTTTTCAGGCAATGACTGATACTTCAAGTGGATCTTCTTCTTCCTCTGTTTCCTCTGTTTATCAGCAAGTTATGGGGAAATTTCCATAG

Coding sequence (CDS)

ATGCAGTTCATGGAGGTCTATGGCGAGCACAGAGCCTCTAAATTTGGTGATGATGATGTTCATGTGGATGTCAATGGTGGCAGCTTGGCTGATAGACGAGCTTCCAAGAGAGGGAGAAATGGGTTCAATATCGCTATGGCTCGGTGCCCGTTGGGTTCGTCGATGGTTCGATCTCCTTTCCTCACAATTCCTTCCGGGTTTAGCCCGACCGTGTTGCTTGATTCGCCGATTATGATATTCCATCTCCAACAACGGGGACGTTTTCCGATACATTCCATAAAGGATGAGAATTCGTTGCTGAATCCTATCATGCTTGAAGATGGGAGTAGCTATGGCAGTGAGGATTCGTACTACAGGTTCACGCCTCGTGGGGAGCTCCGGCTTGAAAATCAGGAAGGTGAAACGGATCATCAGGGGGTCGAATCAGAGAAAACACTGATGGATTATGAATTACTAGCTGAATTTCCCAAAGAGGGTTGTTCTGTATCAGAACAATATGAATTGGGTTCATCCTCCACTGATAAGAACAATGTGGAAACCTGTCTTTCCTCTGTAACTCATAATCAAGACATTGAGCAGCCGGCGGCGCCGCCGCCGCCGCCGTTAGAAACAGAACACAAAGGGTCTTATGTTCCTGTCGGAATGCTAAAAACATCAGAAGATGGATACAACTGGAGAAAGTACGGCCAGAAACAGGTGAAGGGCAGCGAATATCCAAGAAGCTATTACAAATGCACGCATCCAAATTGTATAGTAAAGAAGAAGGTGGAGCGATCGCTTGACGGTCAGATCACAGAGATTATATATACCGGCGCTCATAATCATTCTAAGCCGGACCCCACTCGCCGGGCTGTGCTTGGATCAGTGCTTGCTCCAGATGATGCTCCGGACACCGGCGAAGGCGGTGGAAGCCGCGCCAAACTTGAAGGTGCACTGCCATGGCGGAACAGTCACTATGGGGTGAAGGATTGGAGCGTTGATGGGCTAGAAAGGACATCCTCTGTTTCAGCTGTGACAGAGCTTTCTGAGCCATTGTATGGGAAAACTGTTGGTGGGTTTGAATCCATTGAAACTCCCGAGCTTTCCTCCACTCTTGCTAGCCATGATGATGACGACGGCGGCGGCGGCGGAGGAAGCGGCGGCGGTGGAAACGATGATGATGAACTCACAAATCAGGGGAGTATTTCAGCTGATGATGCAGAGCCTGAGCTGAAAAGAAGACGGAAAGAGGGGATTTCCATGGAGACAAGCTTGGGTTCGCGCTCTGTTCGTGAACAGAGAGTAGTAGTTCAAATCGAAACAGAGTTCGACATACTTGAAGATGGGTATCGATGGAGGAAATATGGCCAAAAAGTTGTGAAAGGAAACCCAAATCCAAGGAGCTATTACAAGTGCACGAGTGCAGGGTGTTCAGTAAGAAAACATGTCGAGAGAGCATCGCATGATTTGAAATGTGTAATAACAACGTACGAAGGAAAGCACAACCATTTAGTGCCAGCAGCGAGGAACAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCACCGCGCAAGCCTCCTCTGCTGCTGCTGCTACTCAGGCGGCGGCGGCGCCGACAAGAAAGTCAGAAACACAAATCCAAGATCTTTCCACACGGTTTTATCCGACACCTGAATTCAGCAGCCATGAGTATCAGCGGTCGTTTGGAACAGCGCCGCCATTTTGTCAGCTGAAGTTCCCTCCATTGCAGACGGCCTTCCCTGCTTTGGACTTCCCCATTTCATTGCCATTGAGCCAAAATCTGTCGGCTTTTCAGGTGTTCTTGGCTGAGAGATTCCTCACGCCAAAACAGGAGCATGAGGAGGATAATATCTATGCTTCTTTTCAGGCAATGACTGATACTTCAAGTGGATCTTCTTCTTCCTCTGTTTCCTCTGTTTATCAGCAAGTTATGGGGAAATTTCCATAG

Protein sequence

MQFMEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTIPSGFSPTVLLDSPIMIFHLQQRGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSYYRFTPRGELRLENQEGETDHQGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNNVETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPDTGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIETPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVERASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQAAAAPTRKSETQIQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLSAFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP
Homology
BLAST of Carg22727 vs. NCBI nr
Match: KAG7022198.1 (WRKY transcription factor WRKY24, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1269.6 bits (3284), Expect = 0.0e+00
Identity = 647/647 (100.00%), Postives = 647/647 (100.00%), Query Frame = 0

Query: 1   MQFMEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPF 60
           MQFMEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPF
Sbjct: 1   MQFMEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPF 60

Query: 61  LTIPSGFSPTVLLDSPIMIFHLQQRGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSYYRF 120
           LTIPSGFSPTVLLDSPIMIFHLQQRGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSYYRF
Sbjct: 61  LTIPSGFSPTVLLDSPIMIFHLQQRGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSYYRF 120

Query: 121 TPRGELRLENQEGETDHQGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNNVET 180
           TPRGELRLENQEGETDHQGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNNVET
Sbjct: 121 TPRGELRLENQEGETDHQGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNNVET 180

Query: 181 CLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSEYPR 240
           CLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSEYPR
Sbjct: 181 CLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSEYPR 240

Query: 241 SYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPDTGE 300
           SYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPDTGE
Sbjct: 241 SYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPDTGE 300

Query: 301 GGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIETPE 360
           GGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIETPE
Sbjct: 301 GGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIETPE 360

Query: 361 LSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETSLGS 420
           LSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETSLGS
Sbjct: 361 LSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETSLGS 420

Query: 421 RSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVERAS 480
           RSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVERAS
Sbjct: 421 RSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVERAS 480

Query: 481 HDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQAAAAPTRKSETQIQD 540
           HDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQAAAAPTRKSETQIQD
Sbjct: 481 HDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQAAAAPTRKSETQIQD 540

Query: 541 LSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLSAFQVFL 600
           LSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLSAFQVFL
Sbjct: 541 LSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLSAFQVFL 600

Query: 601 AERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP 648
           AERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP
Sbjct: 601 AERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP 647

BLAST of Carg22727 vs. NCBI nr
Match: XP_022927490.1 (probable WRKY transcription factor 2 [Cucurbita moschata])

HSP 1 Score: 1236.5 bits (3198), Expect = 0.0e+00
Identity = 636/650 (97.85%), Postives = 638/650 (98.15%), Query Frame = 0

Query: 4   MEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI 63
           MEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI
Sbjct: 1   MEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI 60

Query: 64  PSGFSPTVLLDSPIMIFHLQQ-----RGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSYY 123
           PSGFSPTVLLDSPIM+ +         G FPIHSIKDENSLLNPIMLEDGSSYGSEDSYY
Sbjct: 61  PSGFSPTVLLDSPIMLLNTHDIPSPTTGTFPIHSIKDENSLLNPIMLEDGSSYGSEDSYY 120

Query: 124 RFTPRGELRLENQEGETDH-QGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNN 183
           RFTPRGELRLENQEGETDH QGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNN
Sbjct: 121 RFTPRGELRLENQEGETDHYQGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNN 180

Query: 184 VETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE 243
           VETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE
Sbjct: 181 VETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE 240

Query: 244 YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPD 303
           YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPD
Sbjct: 241 YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPD 300

Query: 304 TGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE 363
           TGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE
Sbjct: 301 TGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE 360

Query: 364 TPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETS 423
           TPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETS
Sbjct: 361 TPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETS 420

Query: 424 LGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVE 483
           LGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVE
Sbjct: 421 LGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVE 480

Query: 484 RASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQAAAAPTRKSETQ 543
           RASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQAAAAPTRKSETQ
Sbjct: 481 RASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQAAAAPTRKSETQ 540

Query: 544 IQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLSAFQ 603
           IQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLSAFQ
Sbjct: 541 IQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLSAFQ 600

Query: 604 VFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP 648
           VFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP
Sbjct: 601 VFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP 650

BLAST of Carg22727 vs. NCBI nr
Match: KAG6588278.1 (putative WRKY transcription factor 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 632/654 (96.64%), Postives = 634/654 (96.94%), Query Frame = 0

Query: 1   MQFMEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPF 60
           MQFMEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPF
Sbjct: 1   MQFMEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPF 60

Query: 61  LTIPSGFSPTVLLDSPIMIFHLQQ-----RGRFPIHSIKDENSLLNPIMLEDGSSYGSED 120
           LTIPSGFSPTVLLDSPIM+ +         G FPIHSIKDENSLLNPIMLEDGSSYGSED
Sbjct: 61  LTIPSGFSPTVLLDSPIMLLNTHDIPSPTTGTFPIHSIKDENSLLNPIMLEDGSSYGSED 120

Query: 121 SYYRFTPRGELRLENQEGETDH-QGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTD 180
           SYYRFTPRGELRLENQEGETDH QGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTD
Sbjct: 121 SYYRFTPRGELRLENQEGETDHYQGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTD 180

Query: 181 KNNVETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVK 240
           KNNVETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVK
Sbjct: 181 KNNVETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVK 240

Query: 241 GSEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDD 300
           GSEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDD
Sbjct: 241 GSEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDD 300

Query: 301 APDTGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFE 360
           APDTGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFE
Sbjct: 301 APDTGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFE 360

Query: 361 SIETPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISM 420
           SIETPELSSTLASHDDDDG      GGGGNDDDELTNQGSISADDAEPELKRRRKEGISM
Sbjct: 361 SIETPELSSTLASHDDDDG------GGGGNDDDELTNQGSISADDAEPELKRRRKEGISM 420

Query: 421 ETSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRK 480
           ETSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRK
Sbjct: 421 ETSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRK 480

Query: 481 HVERASHDLKCVITTYEGKHNHLVPAARN-SSSSSSSSSSTAQASSAAAATQAAAAPTRK 540
           HVERASHDLKCVITTYEGKHNHLVPAARN SSSSSSSSSSTAQASSAA ATQAAAAPTRK
Sbjct: 481 HVERASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSSTAQASSAATATQAAAAPTRK 540

Query: 541 SETQIQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNL 600
           SETQIQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNL
Sbjct: 541 SETQIQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNL 600

Query: 601 SAFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP 648
           SAFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP
Sbjct: 601 SAFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP 648

BLAST of Carg22727 vs. NCBI nr
Match: XP_023003130.1 (WRKY transcription factor SUSIBA2-like [Cucurbita maxima])

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 616/657 (93.76%), Postives = 623/657 (94.82%), Query Frame = 0

Query: 4   MEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI 63
           MEVYGEHRASKFGDDDV VDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI
Sbjct: 1   MEVYGEHRASKFGDDDVLVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI 60

Query: 64  PSGFSPTVLLDSPIMIFHLQQ-----RGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSYY 123
           PSGFSPTVLLDSPIM+ +         G FPIHSIKDENSLLNPIMLEDGSSYGSEDSYY
Sbjct: 61  PSGFSPTVLLDSPIMLLNTHDILSPTTGNFPIHSIKDENSLLNPIMLEDGSSYGSEDSYY 120

Query: 124 RFTPRGELRLENQEGETDH-QGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNN 183
           RFTPRGELR ENQEGETDH QG ESEKTLMDYELLA+FPKE CSV EQYELGSSSTDKNN
Sbjct: 121 RFTPRGELRFENQEGETDHRQGFESEKTLMDYELLADFPKEDCSVLEQYELGSSSTDKNN 180

Query: 184 VETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE 243
           VETCLSSVTHN DIEQP  PPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE
Sbjct: 181 VETCLSSVTHNHDIEQP-PPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE 240

Query: 244 YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPD 303
           YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRA+LGSVLAPDDAPD
Sbjct: 241 YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRALLGSVLAPDDAPD 300

Query: 304 TGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE 363
           TGEGGGSRAKLEG LPWRNS YGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE
Sbjct: 301 TGEGGGSRAKLEGGLPWRNSPYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE 360

Query: 364 TPELSSTLASHDDDD--GGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISME 423
           TPELSST  SHDDDD  GGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISME
Sbjct: 361 TPELSSTFVSHDDDDGGGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISME 420

Query: 424 TSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKH 483
           TSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTS GCSVRKH
Sbjct: 421 TSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSTGCSVRKH 480

Query: 484 VERASHDLKCVITTYEGKHNHLVPAARN-----SSSSSSSSSSTAQASSAAAATQAAAAP 543
           VERASHDLKCVITTYEGKHNHLVPAARN     SSSSSSSSSSTAQASS AAATQAAAAP
Sbjct: 481 VERASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSSSSSSTAQASS-AAATQAAAAP 540

Query: 544 TRKSETQIQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLS 603
           TRKSETQIQDLST+FYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLS
Sbjct: 541 TRKSETQIQDLSTQFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLS 600

Query: 604 QNLSAFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP 648
           QNLSAFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSS SSSSSVSSVY+Q+MGKFP
Sbjct: 601 QNLSAFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSSESSSSSVSSVYKQIMGKFP 655

BLAST of Carg22727 vs. NCBI nr
Match: XP_023520684.1 (WRKY transcription factor WRKY24-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 609/649 (93.84%), Postives = 617/649 (95.07%), Query Frame = 0

Query: 1   MQFMEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPF 60
           + FMEVYGEHRASKFGDDDV VDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPF
Sbjct: 10  IMFMEVYGEHRASKFGDDDVLVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPF 69

Query: 61  LTIPSGFSPTVLLDSPIMIFHLQQ-----RGRFPIHSIKDENSLLNPIMLEDGSSYGSED 120
           LTIPSGFSPTVLLDSPIM+ +         G FPIHSIKDENSLLNPIMLEDGSSYGSED
Sbjct: 70  LTIPSGFSPTVLLDSPIMLLNTHDIPSPTTGTFPIHSIKDENSLLNPIMLEDGSSYGSED 129

Query: 121 SYYRFTPRGELRLENQEGETDHQGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDK 180
           SYYRFTPRGELRL NQEGETDHQ      TLMDYELLA+FPKEGCSV EQYELGSSSTDK
Sbjct: 130 SYYRFTPRGELRLGNQEGETDHQ------TLMDYELLADFPKEGCSVLEQYELGSSSTDK 189

Query: 181 NNVETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKG 240
           NNVETCLSSVTHNQDIEQ A PPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKG
Sbjct: 190 NNVETCLSSVTHNQDIEQAAPPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKG 249

Query: 241 SEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDA 300
           SEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRA+LGSVL+ DDA
Sbjct: 250 SEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRALLGSVLSQDDA 309

Query: 301 PDTGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFES 360
           PDTGEGGGSRAKLEG L WRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFES
Sbjct: 310 PDTGEGGGSRAKLEGGLAWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFES 369

Query: 361 IETPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISME 420
           IETPELSSTLASHDDDDGGG    GGGGNDDDELTNQGSISADDAEPELKRRRKEGISME
Sbjct: 370 IETPELSSTLASHDDDDGGG----GGGGNDDDELTNQGSISADDAEPELKRRRKEGISME 429

Query: 421 TSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKH 480
           TSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKH
Sbjct: 430 TSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKH 489

Query: 481 VERASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASS-AAAATQAAAAPTRKS 540
           VERASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASS AAAATQAAAAPTRKS
Sbjct: 490 VERASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAAATQAAAAPTRKS 549

Query: 541 ETQIQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLS 600
           ETQIQDLST+FYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLS
Sbjct: 550 ETQIQDLSTQFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLS 609

Query: 601 AFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVM 644
           AFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQ +
Sbjct: 610 AFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQTI 648

BLAST of Carg22727 vs. ExPASy Swiss-Prot
Match: Q9FG77 (Probable WRKY transcription factor 2 OS=Arabidopsis thaliana OX=3702 GN=WRKY2 PE=1 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.1e-76
Identity = 215/544 (39.52%), Postives = 282/544 (51.84%), Query Frame = 0

Query: 9   EHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGS-----SMVRSPFLTI 68
           E  +S    D    +V  G L++R A++ G N   +        +     S +RSP LTI
Sbjct: 55  EDTSSNHNKDSSQSNVFRGGLSERIAARAGFNAPRLNTENIRTNTDFSIDSNLRSPCLTI 114

Query: 69  PS-GFSPTVLLDSPIMIFH-LQQ----RGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSY 128
            S G SP  LL+SP+ + + L Q     G+FP     + N+L +    ++   +    + 
Sbjct: 115 SSPGLSPATLLESPVFLSNPLAQPSPTTGKFPFLPGVNGNALSSEKAKDE--FFDDIGAS 174

Query: 129 YRFTPRGELRLENQEGETDHQGVE--SEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDK 188
           + F P         +G T+   V+  +          AE  K G    E   L    TD 
Sbjct: 175 FSFHPVSRSSSSFFQGTTEMMSVDYGNYNNRSSSHQSAEEVKPGSENIESSNLYGIETDN 234

Query: 189 NNVETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKG 248
            N +   S VT N  +E          E + +G  +  G    +EDGYNWRKYGQK VKG
Sbjct: 235 QNGQNKTSDVTTNTSLE--TVDHQEEEEEQRRGDSMAGG--APAEDGYNWRKYGQKLVKG 294

Query: 249 SEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDA 308
           SEYPRSYYKCT+PNC VKKKVERS +G ITEIIY GAHNH KP P RR+ +         
Sbjct: 295 SEYPRSYYKCTNPNCQVKKKVERSREGHITEIIYKGAHNHLKPPPNRRSGM-------QV 354

Query: 309 PDTGEGGGSRAKLEGALPW--RNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGF 368
             T +    + + + A  W   N+       + + +E  S+       S  +  +T G +
Sbjct: 355 DGTEQVEQQQQQRDSAATWVSCNNTQQQGGSNENNVEEGSTRFEYGNQSGSIQAQTGGQY 414

Query: 369 ES---IETPELSSTLASHDDDDGGG----------GGGSGGGGNDDDELTNQGSISADDA 428
           ES   +   + SST ++ +D+D  G          GGG GGGG  D              
Sbjct: 415 ESGDPVVVVDASSTFSNDEDEDDRGTHGSVSLGYDGGGGGGGGEGD-------------- 474

Query: 429 EPELKRRRKEGISMETSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPR 488
           E E KRR+ E  + E S  +R++RE RVVVQ  ++ DIL+DGYRWRKYGQKVVKGNPNPR
Sbjct: 475 ESESKRRKLEAFAAEMSGSTRAIREPRVVVQTTSDVDILDDGYRWRKYGQKVVKGNPNPR 534

Query: 489 SYYKCTSAGCSVRKHVERASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSA 525
           SYYKCT+ GC+VRKHVERASHDLK VITTYEGKHNH VPAARNSS      S    +  +
Sbjct: 535 SYYKCTAPGCTVRKHVERASHDLKSVITTYEGKHNHDVPAARNSSHGGGGDSGNGNSGGS 571

BLAST of Carg22727 vs. ExPASy Swiss-Prot
Match: Q6B6R4 (WRKY transcription factor WRKY24 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY24 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 7.9e-67
Identity = 163/313 (52.08%), Postives = 191/313 (61.02%), Query Frame = 0

Query: 212 PVGMLKTSEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTG 271
           P    ++S+DGYNWRKYGQKQVKGSE PRSYYKCT PNC  KKKVERSLDGQITEI+Y G
Sbjct: 212 PQSQRRSSDDGYNWRKYGQKQVKGSENPRSYYKCTFPNCPTKKKVERSLDGQITEIVYKG 271

Query: 272 AHNHSKPDPTRRAVLGSVLAPDDAPDTGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLER 331
            HNH+KP  TRR                  G S A++                       
Sbjct: 272 THNHAKPQNTRR----------------NSGSSAAQV----------------------- 331

Query: 332 TSSVSAVTELSEPLYGKTVGGFESIETPELSSTLASHDDDDGGGGGGSGGGGNDDDELTN 391
              + +  ++SE  +G   G   +  TPE SS  AS  DD+ G G    G G  D+    
Sbjct: 332 ---LQSGGDMSEHSFGGMSG---TAATPENSS--ASFGDDEIGVGSPRAGNGGGDE---- 391

Query: 392 QGSISADDAEPELKRRRK----EGISMETSLGSRSVREQRVVVQIETEFDILEDGYRWRK 451
                 DD EP+ KR RK    EGISM    G+R+VRE RVVVQ  ++ DIL+DGYRWRK
Sbjct: 392 -----FDDDEPDSKRWRKDGDGEGISM---AGNRTVREPRVVVQTMSDIDILDDGYRWRK 451

Query: 452 YGQKVVKGNPNPRSYYKCTSAGCSVRKHVERASHDLKCVITTYEGKHNHLVPAARNSSSS 511
           YGQKVVKGNPNPRSYYKCT+AGC VRKHVERASHDL+ VITTYEGKHNH VPAAR S++ 
Sbjct: 452 YGQKVVKGNPNPRSYYKCTTAGCPVRKHVERASHDLRAVITTYEGKHNHDVPAARGSAAL 465

Query: 512 SSSSSSTAQASSA 521
              +   A A+S+
Sbjct: 512 YRPAPPAAAATSS 465

BLAST of Carg22727 vs. ExPASy Swiss-Prot
Match: Q6IEQ7 (WRKY transcription factor WRKY24 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY24 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 6.7e-66
Identity = 162/313 (51.76%), Postives = 190/313 (60.70%), Query Frame = 0

Query: 212 PVGMLKTSEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTG 271
           P    ++S+DGYNWRKYGQKQVKGSE PRSYYKCT PNC  KKKVERSLDGQITEI+Y G
Sbjct: 212 PQSQRRSSDDGYNWRKYGQKQVKGSENPRSYYKCTFPNCPTKKKVERSLDGQITEIVYKG 271

Query: 272 AHNHSKPDPTRRAVLGSVLAPDDAPDTGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLER 331
            HNH+KP  TRR                  G S A++                       
Sbjct: 272 THNHAKPQNTRR----------------NSGSSAAQV----------------------- 331

Query: 332 TSSVSAVTELSEPLYGKTVGGFESIETPELSSTLASHDDDDGGGGGGSGGGGNDDDELTN 391
              + +  ++SE  +G   G   +  TPE SS  AS  DD+   G    G G  D+    
Sbjct: 332 ---LQSGGDMSEHSFGGMSG---TAATPENSS--ASFGDDEIRVGSPRAGNGGGDE---- 391

Query: 392 QGSISADDAEPELKRRRK----EGISMETSLGSRSVREQRVVVQIETEFDILEDGYRWRK 451
                 DD EP+ KR RK    EGISM    G+R+VRE RVVVQ  ++ DIL+DGYRWRK
Sbjct: 392 -----FDDDEPDSKRWRKDGDGEGISM---AGNRTVREPRVVVQTMSDIDILDDGYRWRK 451

Query: 452 YGQKVVKGNPNPRSYYKCTSAGCSVRKHVERASHDLKCVITTYEGKHNHLVPAARNSSSS 511
           YGQKVVKGNPNPRSYYKCT+AGC VRKHVERASHDL+ VITTYEGKHNH VPAAR S++ 
Sbjct: 452 YGQKVVKGNPNPRSYYKCTTAGCPVRKHVERASHDLRAVITTYEGKHNHDVPAARGSAAL 465

Query: 512 SSSSSSTAQASSA 521
              +   A A+S+
Sbjct: 512 YRPAPPAAAATSS 465

BLAST of Carg22727 vs. ExPASy Swiss-Prot
Match: Q93WV0 (Probable WRKY transcription factor 20 OS=Arabidopsis thaliana OX=3702 GN=WRKY20 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.5e-65
Identity = 193/503 (38.37%), Postives = 244/503 (48.51%), Query Frame = 0

Query: 27  GSLADRRASKRGRNGFN-----IAMARCPLGSSMVRSPFLTIPSGFSPTVLLDSPIMIFH 86
           G L  +  S  G  G       ++ A+ P+     RS  +TIP G SPT  L+SP+ I +
Sbjct: 19  GDLTAKHDSAGGNGGGGARYKLMSPAKLPIS----RSTDITIPPGLSPTSFLESPVFISN 78

Query: 87  LQQRGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSYYRFTPRGELRLENQEGETDHQGVE 146
           ++              SL  P  +   +S  S      +T RG  +    E ++      
Sbjct: 79  IKP------EPSPTTGSLFKPRPVHISASSSS------YTGRGFHQNTFTEQKSSEFEFR 138

Query: 147 SEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNNVETCLSSVTHNQDIEQPAAPPPPP 206
              + M Y  L +   E     +    GSS +         SS++         + P PP
Sbjct: 139 PPASNMVYAELGKIRSEPPVHFQGQGHGSSHSP--------SSISDAAGSSSELSRPTPP 198

Query: 207 LETEHKGSYVPVG-----MLKTS-------------EDGYNWRKYGQKQVKGSEYPRSYY 266
            +     S +P G      ++TS             +DGYNWRKYGQK VKGSE+PRSYY
Sbjct: 199 CQMTPTSSDIPAGSDQEESIQTSQNDSRGSTPSILADDGYNWRKYGQKHVKGSEFPRSYY 258

Query: 267 KCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPDTGEGGG 326
           KCTHPNC VKK  ERS DGQIT+IIY G H+H KP P RR                  GG
Sbjct: 259 KCTHPNCEVKKLFERSHDGQITDIIYKGTHDHPKPQPGRR----------------NSGG 318

Query: 327 SRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIETPELSS 386
             A+ E    + +S             R    S V  LS P         E    PE+  
Sbjct: 319 MAAQEERLDKYPSS-----------TGRDEKGSGVYNLSNP--------NEQTGNPEVPP 378

Query: 387 TLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETSLGSRSV 446
             AS   DDGG    S           N+     DD +P  KRRR EG +ME +   + +
Sbjct: 379 ISAS---DDGGEAAAS-----------NRNKDEPDDDDPFSKRRRMEG-AMEITPLVKPI 438

Query: 447 REQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVERASHDL 506
           RE RVVVQ  +E DIL+DGYRWRKYGQKVV+GNPNPRSYYKCT+ GC VRKHVERASHD 
Sbjct: 439 REPRVVVQTLSEVDILDDGYRWRKYGQKVVRGNPNPRSYYKCTAHGCPVRKHVERASHDP 447

BLAST of Carg22727 vs. ExPASy Swiss-Prot
Match: Q6VWJ6 (WRKY transcription factor SUSIBA2 OS=Hordeum vulgare OX=4513 GN=WRKY46 PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 7.4e-65
Identity = 192/495 (38.79%), Postives = 253/495 (51.11%), Query Frame = 0

Query: 47  ARCPLGSSMVRSPFLTIPSGFSPTVLLDSPIMIFHLQ-----QRGRFPIHSIKDENSLLN 106
           AR P+     R   LTIP+GFSP+ LLDSP+++ + +       G   + +I  +++  +
Sbjct: 4   ARLPIS----RESCLTIPAGFSPSALLDSPVLLTNFKVEPSPTTGSLGMAAILHKSA--H 63

Query: 107 PIMLEDGSSYGSEDSYYRFTPRGELRLENQEGETDHQGVESEKTLMDYELLAEFPKEGCS 166
           P ML         +++     RG    E +     H    S+        +++  K   S
Sbjct: 64  PDMLPSPRDKSVRNAH---EDRGSRDFEFK----PHLNSSSQSLA---PAMSDLKKHEHS 123

Query: 167 VSEQYELGSSSTD---KNNVETCL--SSVT-----HNQDIEQPAAPPPPPLETEHKGSYV 226
           +  Q    SSS+      N   C   SS+T      NQ +         P E    G+  
Sbjct: 124 MQNQSMNPSSSSSNMVNENRPPCSRESSLTVNVSAQNQPVGMVGLTDSMPAEV---GTSE 183

Query: 227 PVGML----------------KTSEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCIVKKK 286
           P  M                 K+++DGYNWRKYGQK VKGSE PRSYYKCTHPNC VKK 
Sbjct: 184 PQQMNSSDNAMQEPQSENVADKSADDGYNWRKYGQKHVKGSENPRSYYKCTHPNCEVKKL 243

Query: 287 VERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPDTGEGGGSRAKLEGALPWR 346
           +ER++DG ITE++Y G HNH KP P RR   G+V  P +  +    G S A         
Sbjct: 244 LERAVDGLITEVVYKGRHNHPKPQPNRRLAGGAV--PSNQGEERYDGASAAD-------- 303

Query: 347 NSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIETPELSSTLASHDDDDGGG 406
                             S +A++ L+ P++  + G  E +         AS  DDD   
Sbjct: 304 ----------------DKSSNALSNLANPVH--SPGMVEPVP--------ASVSDDDIDA 363

Query: 407 GGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETSLGSRSVREQRVVVQIETE 466
           GGG    G+D          + ++ + E KRR+ E   ++ +L  +  RE RVVVQ  +E
Sbjct: 364 GGGRPYPGDD----------ATEEEDLESKRRKMESAGIDAALMGKPNREPRVVVQTVSE 423

Query: 467 FDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVERASHDLKCVITTYEGKHN 511
            DIL+DGYRWRKYGQKVVKGNPNPRSYYKCTS GC VRKHVERASHD K VITTYEGKHN
Sbjct: 424 VDILDDGYRWRKYGQKVVKGNPNPRSYYKCTSTGCPVRKHVERASHDPKSVITTYEGKHN 433

BLAST of Carg22727 vs. ExPASy TrEMBL
Match: A0A6J1EP38 (probable WRKY transcription factor 2 OS=Cucurbita moschata OX=3662 GN=LOC111434299 PE=4 SV=1)

HSP 1 Score: 1236.5 bits (3198), Expect = 0.0e+00
Identity = 636/650 (97.85%), Postives = 638/650 (98.15%), Query Frame = 0

Query: 4   MEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI 63
           MEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI
Sbjct: 1   MEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI 60

Query: 64  PSGFSPTVLLDSPIMIFHLQQ-----RGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSYY 123
           PSGFSPTVLLDSPIM+ +         G FPIHSIKDENSLLNPIMLEDGSSYGSEDSYY
Sbjct: 61  PSGFSPTVLLDSPIMLLNTHDIPSPTTGTFPIHSIKDENSLLNPIMLEDGSSYGSEDSYY 120

Query: 124 RFTPRGELRLENQEGETDH-QGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNN 183
           RFTPRGELRLENQEGETDH QGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNN
Sbjct: 121 RFTPRGELRLENQEGETDHYQGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNN 180

Query: 184 VETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE 243
           VETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE
Sbjct: 181 VETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE 240

Query: 244 YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPD 303
           YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPD
Sbjct: 241 YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPD 300

Query: 304 TGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE 363
           TGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE
Sbjct: 301 TGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE 360

Query: 364 TPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETS 423
           TPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETS
Sbjct: 361 TPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETS 420

Query: 424 LGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVE 483
           LGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVE
Sbjct: 421 LGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVE 480

Query: 484 RASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQAAAAPTRKSETQ 543
           RASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQAAAAPTRKSETQ
Sbjct: 481 RASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQAAAAPTRKSETQ 540

Query: 544 IQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLSAFQ 603
           IQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLSAFQ
Sbjct: 541 IQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLSQNLSAFQ 600

Query: 604 VFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP 648
           VFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP
Sbjct: 601 VFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP 650

BLAST of Carg22727 vs. ExPASy TrEMBL
Match: A0A6J1KQX2 (WRKY transcription factor SUSIBA2-like OS=Cucurbita maxima OX=3661 GN=LOC111496831 PE=4 SV=1)

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 616/657 (93.76%), Postives = 623/657 (94.82%), Query Frame = 0

Query: 4   MEVYGEHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI 63
           MEVYGEHRASKFGDDDV VDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI
Sbjct: 1   MEVYGEHRASKFGDDDVLVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTI 60

Query: 64  PSGFSPTVLLDSPIMIFHLQQ-----RGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSYY 123
           PSGFSPTVLLDSPIM+ +         G FPIHSIKDENSLLNPIMLEDGSSYGSEDSYY
Sbjct: 61  PSGFSPTVLLDSPIMLLNTHDILSPTTGNFPIHSIKDENSLLNPIMLEDGSSYGSEDSYY 120

Query: 124 RFTPRGELRLENQEGETDH-QGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNN 183
           RFTPRGELR ENQEGETDH QG ESEKTLMDYELLA+FPKE CSV EQYELGSSSTDKNN
Sbjct: 121 RFTPRGELRFENQEGETDHRQGFESEKTLMDYELLADFPKEDCSVLEQYELGSSSTDKNN 180

Query: 184 VETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE 243
           VETCLSSVTHN DIEQP  PPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE
Sbjct: 181 VETCLSSVTHNHDIEQP-PPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSE 240

Query: 244 YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPD 303
           YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRA+LGSVLAPDDAPD
Sbjct: 241 YPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRALLGSVLAPDDAPD 300

Query: 304 TGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE 363
           TGEGGGSRAKLEG LPWRNS YGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE
Sbjct: 301 TGEGGGSRAKLEGGLPWRNSPYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIE 360

Query: 364 TPELSSTLASHDDDD--GGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISME 423
           TPELSST  SHDDDD  GGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISME
Sbjct: 361 TPELSSTFVSHDDDDGGGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISME 420

Query: 424 TSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKH 483
           TSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTS GCSVRKH
Sbjct: 421 TSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSTGCSVRKH 480

Query: 484 VERASHDLKCVITTYEGKHNHLVPAARN-----SSSSSSSSSSTAQASSAAAATQAAAAP 543
           VERASHDLKCVITTYEGKHNHLVPAARN     SSSSSSSSSSTAQASS AAATQAAAAP
Sbjct: 481 VERASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSSSSSSTAQASS-AAATQAAAAP 540

Query: 544 TRKSETQIQDLSTRFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLS 603
           TRKSETQIQDLST+FYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLS
Sbjct: 541 TRKSETQIQDLSTQFYPTPEFSSHEYQRSFGTAPPFCQLKFPPLQTAFPALDFPISLPLS 600

Query: 604 QNLSAFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSSGSSSSSVSSVYQQVMGKFP 648
           QNLSAFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSS SSSSSVSSVY+Q+MGKFP
Sbjct: 601 QNLSAFQVFLAERFLTPKQEHEEDNIYASFQAMTDTSSESSSSSVSSVYKQIMGKFP 655

BLAST of Carg22727 vs. ExPASy TrEMBL
Match: A0A6J1HD23 (WRKY transcription factor SUSIBA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461729 PE=4 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 6.6e-218
Identity = 451/758 (59.50%), Postives = 524/758 (69.13%), Query Frame = 0

Query: 1   MQFMEVYGEHRAS------KFGDDDVHVDVNGGSLADRRASKRGRNGFNIA--------- 60
           M+FM+  GE+  S      KF DD+V VD N GSL   RASKRGRNGFN++         
Sbjct: 1   MRFMDDNGENSRSPTSSTPKFSDDEVLVDANAGSL---RASKRGRNGFNVSKNNLALPRF 60

Query: 61  MARCPLGSSMVRSPFLTIPSGFSPTVLLDSPIMIFHLQQ-----RGRFPIHSIKDENSLL 120
               PLGS MVRSP LTIP G SPT+LLDSPI + + Q       G FPIH IKDE+SLL
Sbjct: 61  RTATPLGSPMVRSPCLTIPPGISPTLLLDSPITLLNTQDLPSPTTGTFPIHQIKDEHSLL 120

Query: 121 NPIMLEDGSSYGSEDSYYRFTPRGEL-------RLENQEGETDHQGVESEKTLMDYELLA 180
           NP+M EDGSS+GSEDSY+RFTP GEL       RLENQE + D Q  ESEK LMD+E L 
Sbjct: 121 NPVMPEDGSSHGSEDSYFRFTPHGELNTLQSLFRLENQEADIDRQAFESEKALMDFEFLT 180

Query: 181 EFPKEGCSVSEQYELGSSSTDK-----------NNVETCLSSVTHNQ------DIEQPAA 240
           +F KEG  +  +Y++ SS+ +            +N+E+CLS++ +NQ        ++  +
Sbjct: 181 DFSKEGSVL--KYDIASSTGNNHFDGKSVNGNCDNMESCLSAIANNQPSTPEESAKRDDS 240

Query: 241 PPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCIVKKKV 300
               PLE E KGS +P+GML+TSEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNC VKKKV
Sbjct: 241 ETQHPLEGEQKGSCIPMGMLRTSEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCQVKKKV 300

Query: 301 ERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPDTGEGGGSRAKLEGALPWRN 360
           ERSLDGQITEIIY GAH H+KPDP RRA+LGS+L PDD P+ GEGGG+RAK+E  L WRN
Sbjct: 301 ERSLDGQITEIIYKGAHIHAKPDPNRRAMLGSMLMPDDTPEIGEGGGNRAKVEAGLTWRN 360

Query: 361 SHYGVK------DWSVDGLERTSSVSAVTELSEPL---YGKTVGGFESIETPELSSTLAS 420
           + YGVK      DWSVDGLERTSS+S VTELS+PL    GK VG FES+ TPELSSTLAS
Sbjct: 361 TQYGVKDTKPISDWSVDGLERTSSISVVTELSDPLLNPQGKNVGVFESVGTPELSSTLAS 420

Query: 421 HDDDDGGGGGGSGGGGNDDDELTNQGSIS----ADDAEPELKRRRKEGISMETSLGSRSV 480
           HDDD        GGGG DDD+LT QGSIS    ADDAEPELKRRRKEG S+ET+L SRS+
Sbjct: 421 HDDD--------GGGGGDDDDLTTQGSISVCIEADDAEPELKRRRKEGGSIETNLASRSL 480

Query: 481 REQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVERASHDL 540
           RE RVVVQIET+ DILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGC+VRKHVERASHDL
Sbjct: 481 REPRVVVQIETDVDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCAVRKHVERASHDL 540

Query: 541 KCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQ--AAAAPTRKSETQIQDL 600
           KCVITTYEGKHNH VPAARN S  +SS+ ++  ++S        +  +    SETQIQDL
Sbjct: 541 KCVITTYEGKHNHEVPAARNGSQVNSSNGNSQPSTSHVQPNMDLSRNSNVANSETQIQDL 600

Query: 601 STRFYPTPEFSSHEYQRS--FGT--------APPFCQLKFPPLQTAFP------------ 648
           + +FYP PEF +++YQRS  F T        AP FCQ+KFP L+   P            
Sbjct: 601 AAQFYPKPEF-NYDYQRSGCFDTFRNDIKLGAPSFCQMKFPQLRHTLPYSSFGLNSKHTS 660

BLAST of Carg22727 vs. ExPASy TrEMBL
Match: A0A6J1JK30 (WRKY transcription factor WRKY24-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485817 PE=4 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 1.1e-217
Identity = 450/758 (59.37%), Postives = 523/758 (69.00%), Query Frame = 0

Query: 1   MQFMEVYGEH------RASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIA--------- 60
           M+FM+  GE+        SKF DD+V VD NGGSL   RASKRGRNGFN++         
Sbjct: 1   MRFMDDNGENSRSPTSSTSKFSDDEVLVDANGGSL---RASKRGRNGFNVSKNNLALPRF 60

Query: 61  MARCPLGSSMVRSPFLTIPSGFSPTVLLDSPIMIFHLQQ-----RGRFPIHSIKDENSLL 120
               PLGS MVRSP LTIP G SPT+LLDSPI + + Q       G FPIH IKDE+SLL
Sbjct: 61  RTASPLGSPMVRSPCLTIPPGISPTLLLDSPITLLNTQDLPSPTTGTFPIHQIKDEHSLL 120

Query: 121 NPIMLEDGSSYGSEDSYYRFTPRGEL-------RLENQEGETDHQGVESEKTLMDYELLA 180
           NP+M EDGSS+GSEDSY+RFTP GEL       RLENQE + D Q  ESEK LMD+E L 
Sbjct: 121 NPVMPEDGSSHGSEDSYFRFTPHGELNTLQSLFRLENQEADIDRQAFESEKALMDFEFLT 180

Query: 181 EFPKEGCSVSEQYELGSSSTDK-----------NNVETCLSSVTHNQ------DIEQPAA 240
           +F KEG  +  +Y++ SS+ +            +N+E+CLS++ HNQ        ++  +
Sbjct: 181 DFSKEGSVL--KYDIASSTGNNHFDGKSVNGNCDNMESCLSAIAHNQPSTPEESAKRDDS 240

Query: 241 PPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCIVKKKV 300
               PLE E KGS +P+GML+TSEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNC VKKKV
Sbjct: 241 ETQHPLEGEQKGSCIPMGMLRTSEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCQVKKKV 300

Query: 301 ERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPDTGEGGGSRAKLEGALPWRN 360
           ERSLDGQITEIIY GAH H+KPDP RRA+LGS+L PDD P+ GEGGG+RAK+E  L WRN
Sbjct: 301 ERSLDGQITEIIYKGAHIHAKPDPNRRAMLGSMLMPDDTPEIGEGGGNRAKVEAGLTWRN 360

Query: 361 SHYGVK------DWSVDGLERTSSVSAVTELSEPL---YGKTVGGFESIETPELSSTLAS 420
           + YGVK      DWSVDGLERTSS+S VTELS+PL    GK VG FES+ TPELSSTLAS
Sbjct: 361 TQYGVKDTKPISDWSVDGLERTSSISVVTELSDPLLNPQGKNVGVFESVGTPELSSTLAS 420

Query: 421 HDDDDGGGGGGSGGGGNDDDELTNQGSIS----ADDAEPELKRRRKEGISMETSLGSRSV 480
           HDDD        G GG DDD+LT QGSIS    ADDAEPELKRRRKEG S+ET+L SRS+
Sbjct: 421 HDDD--------GSGGGDDDDLTTQGSISVCIEADDAEPELKRRRKEGGSIETNLASRSL 480

Query: 481 REQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVERASHDL 540
           RE RVVVQIET+ DILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGC+VRKHVERASHDL
Sbjct: 481 REPRVVVQIETDVDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCAVRKHVERASHDL 540

Query: 541 KCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSAAAATQ--AAAAPTRKSETQIQDL 600
           KCVITTYEGKHNH VPAARN S  +SS+ ++  ++S        +  +    SE+QIQDL
Sbjct: 541 KCVITTYEGKHNHEVPAARNGSQVNSSNGNSQPSTSHVQPNMDLSRNSNVTNSESQIQDL 600

Query: 601 STRFYPTPEFSSHEYQRS--FGT--------APPFCQLKFPPLQTAFP------------ 648
           + +FY  PEF +++YQRS  F T        AP FCQ+KFP L+   P            
Sbjct: 601 AAQFYSKPEF-NYDYQRSGCFDTFRNDIKLGAPSFCQMKFPQLRHTLPYSSFGLNSKHTS 660

BLAST of Carg22727 vs. ExPASy TrEMBL
Match: A0A0A0M0L1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G657480 PE=4 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 7.6e-206
Identity = 428/726 (58.95%), Postives = 495/726 (68.18%), Query Frame = 0

Query: 17  DDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGSSMVRSPFLTIPSGFSPTVLLDSP 76
           DD V VD N GS A  R SKR RNG+   +      S ++ SP LTIP G +PT+LLDSP
Sbjct: 9   DDGVLVDANAGSFAQSRPSKRRRNGW---IPTKDSHSPILSSPSLTIPPGINPTLLLDSP 68

Query: 77  IMIFHLQQ-----RGRF-PIHSIKDENSLLNPIMLEDGSSYGSEDSYYRFTPRGE----- 136
           +M+ + Q       G F PIH IKDE SLLNP+M EDG S+GSEDS++RF P+GE     
Sbjct: 69  VMLLNTQDLPSPTTGTFPPIHQIKDEQSLLNPVMPEDGISHGSEDSFFRFAPQGELCTLQ 128

Query: 137 --LRLENQEGETDHQGVESEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDK-------- 196
             LR+ENQE E DHQ +ESEKTLMD+E + + PKE   +  +YE+  S+ +         
Sbjct: 129 SLLRIENQEAEIDHQALESEKTLMDFEFVPDIPKEAAVL--KYEIAPSTDNSYFDGKIVN 188

Query: 197 ---NNVETCLSSVT------HNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWR 256
               N+E+CLSS+T      H +  +        PLE E KGSY+P+GML+TSEDGYNWR
Sbjct: 189 GNCENMESCLSSITTNQPCIHEESTQGDDIDTQHPLEDEQKGSYIPMGMLRTSEDGYNWR 248

Query: 257 KYGQKQVKGSEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVL 316
           KYGQKQVKGSEYPRSYYKCTHPNC+VKKKVERSLDGQITEIIY GAHNH+KPDP RRA+ 
Sbjct: 249 KYGQKQVKGSEYPRSYYKCTHPNCLVKKKVERSLDGQITEIIYKGAHNHAKPDPNRRAMA 308

Query: 317 GSVLAPDDAPDTGEGGGSRAKLEGALPWRNSHYGVKD------WSVDGLERTSSVSAVTE 376
           GSV    D P+ GEGGG+ +KLE  L WRNS YGVKD       SVDGLERT SVS ++E
Sbjct: 309 GSVPISGDNPEIGEGGGNHSKLEAGLTWRNSQYGVKDIKPISNCSVDGLERTPSVSVLSE 368

Query: 377 LSEPLYG---KTVGGFESIETPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSIS- 436
           LS+PL     KTVG  E + TPELSSTLASHDDD+GGGG         DD+LT QGSIS 
Sbjct: 369 LSDPLLNPQEKTVGVLEPVGTPELSSTLASHDDDNGGGG---------DDDLTTQGSISV 428

Query: 437 ---ADDAEPELKRRRKEGISMETSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVV 496
              ADDAEPELKRRRKE  S+ET+L SRSVRE RVVVQIETE DILEDGYRWRKYGQKVV
Sbjct: 429 CTEADDAEPELKRRRKEDSSIETNLASRSVREPRVVVQIETEVDILEDGYRWRKYGQKVV 488

Query: 497 KGNPNPRSYYKCTSAGCSVRKHVERASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSS 556
           KGNPNPRSYYKCTSAGC VRKHVERASHDLKCVITTYEGKHNH VPAARNSS  +S + +
Sbjct: 489 KGNPNPRSYYKCTSAGCLVRKHVERASHDLKCVITTYEGKHNHEVPAARNSSQVNSGNGN 548

Query: 557 TAQASSAAAATQAAAAPTR--KSETQIQDLSTRFYPTPEFSSHEYQRS-FGT-------- 616
               +S        +  +   KSET+IQDL+T FYP PEF +++YQRS F T        
Sbjct: 549 AQPPASHVQPNMGLSRNSNVPKSETEIQDLATHFYPKPEF-NNDYQRSGFDTFTNDMKLG 608

Query: 617 APPFCQLKFPPLQTAFP----------------------ALDFPISLPLSQNLSA----- 647
           APPFCQ+KFPPL+   P                        DFPISLPL+Q LSA     
Sbjct: 609 APPFCQMKFPPLRNTLPYSTFGLSSKHTATGISGSLASVVSDFPISLPLNQKLSAAGYDY 668

BLAST of Carg22727 vs. TAIR 10
Match: AT5G56270.1 (WRKY DNA-binding protein 2 )

HSP 1 Score: 289.3 bits (739), Expect = 7.8e-78
Identity = 215/544 (39.52%), Postives = 282/544 (51.84%), Query Frame = 0

Query: 9   EHRASKFGDDDVHVDVNGGSLADRRASKRGRNGFNIAMARCPLGS-----SMVRSPFLTI 68
           E  +S    D    +V  G L++R A++ G N   +        +     S +RSP LTI
Sbjct: 55  EDTSSNHNKDSSQSNVFRGGLSERIAARAGFNAPRLNTENIRTNTDFSIDSNLRSPCLTI 114

Query: 69  PS-GFSPTVLLDSPIMIFH-LQQ----RGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSY 128
            S G SP  LL+SP+ + + L Q     G+FP     + N+L +    ++   +    + 
Sbjct: 115 SSPGLSPATLLESPVFLSNPLAQPSPTTGKFPFLPGVNGNALSSEKAKDE--FFDDIGAS 174

Query: 129 YRFTPRGELRLENQEGETDHQGVE--SEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDK 188
           + F P         +G T+   V+  +          AE  K G    E   L    TD 
Sbjct: 175 FSFHPVSRSSSSFFQGTTEMMSVDYGNYNNRSSSHQSAEEVKPGSENIESSNLYGIETDN 234

Query: 189 NNVETCLSSVTHNQDIEQPAAPPPPPLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKG 248
            N +   S VT N  +E          E + +G  +  G    +EDGYNWRKYGQK VKG
Sbjct: 235 QNGQNKTSDVTTNTSLE--TVDHQEEEEEQRRGDSMAGG--APAEDGYNWRKYGQKLVKG 294

Query: 249 SEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDA 308
           SEYPRSYYKCT+PNC VKKKVERS +G ITEIIY GAHNH KP P RR+ +         
Sbjct: 295 SEYPRSYYKCTNPNCQVKKKVERSREGHITEIIYKGAHNHLKPPPNRRSGM-------QV 354

Query: 309 PDTGEGGGSRAKLEGALPW--RNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGF 368
             T +    + + + A  W   N+       + + +E  S+       S  +  +T G +
Sbjct: 355 DGTEQVEQQQQQRDSAATWVSCNNTQQQGGSNENNVEEGSTRFEYGNQSGSIQAQTGGQY 414

Query: 369 ES---IETPELSSTLASHDDDDGGG----------GGGSGGGGNDDDELTNQGSISADDA 428
           ES   +   + SST ++ +D+D  G          GGG GGGG  D              
Sbjct: 415 ESGDPVVVVDASSTFSNDEDEDDRGTHGSVSLGYDGGGGGGGGEGD-------------- 474

Query: 429 EPELKRRRKEGISMETSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPR 488
           E E KRR+ E  + E S  +R++RE RVVVQ  ++ DIL+DGYRWRKYGQKVVKGNPNPR
Sbjct: 475 ESESKRRKLEAFAAEMSGSTRAIREPRVVVQTTSDVDILDDGYRWRKYGQKVVKGNPNPR 534

Query: 489 SYYKCTSAGCSVRKHVERASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSSTAQASSA 525
           SYYKCT+ GC+VRKHVERASHDLK VITTYEGKHNH VPAARNSS      S    +  +
Sbjct: 535 SYYKCTAPGCTVRKHVERASHDLKSVITTYEGKHNHDVPAARNSSHGGGGDSGNGNSGGS 571

BLAST of Carg22727 vs. TAIR 10
Match: AT4G26640.2 (WRKY family transcription factor family protein )

HSP 1 Score: 251.5 bits (641), Expect = 1.8e-66
Identity = 193/503 (38.37%), Postives = 244/503 (48.51%), Query Frame = 0

Query: 27  GSLADRRASKRGRNGFN-----IAMARCPLGSSMVRSPFLTIPSGFSPTVLLDSPIMIFH 86
           G L  +  S  G  G       ++ A+ P+     RS  +TIP G SPT  L+SP+ I +
Sbjct: 19  GDLTAKHDSAGGNGGGGARYKLMSPAKLPIS----RSTDITIPPGLSPTSFLESPVFISN 78

Query: 87  LQQRGRFPIHSIKDENSLLNPIMLEDGSSYGSEDSYYRFTPRGELRLENQEGETDHQGVE 146
           ++              SL  P  +   +S  S      +T RG  +    E ++      
Sbjct: 79  IKP------EPSPTTGSLFKPRPVHISASSSS------YTGRGFHQNTFTEQKSSEFEFR 138

Query: 147 SEKTLMDYELLAEFPKEGCSVSEQYELGSSSTDKNNVETCLSSVTHNQDIEQPAAPPPPP 206
              + M Y  L +   E     +    GSS +         SS++         + P PP
Sbjct: 139 PPASNMVYAELGKIRSEPPVHFQGQGHGSSHSP--------SSISDAAGSSSELSRPTPP 198

Query: 207 LETEHKGSYVPVG-----MLKTS-------------EDGYNWRKYGQKQVKGSEYPRSYY 266
            +     S +P G      ++TS             +DGYNWRKYGQK VKGSE+PRSYY
Sbjct: 199 CQMTPTSSDIPAGSDQEESIQTSQNDSRGSTPSILADDGYNWRKYGQKHVKGSEFPRSYY 258

Query: 267 KCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPDTGEGGG 326
           KCTHPNC VKK  ERS DGQIT+IIY G H+H KP P RR                  GG
Sbjct: 259 KCTHPNCEVKKLFERSHDGQITDIIYKGTHDHPKPQPGRR----------------NSGG 318

Query: 327 SRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEPLYGKTVGGFESIETPELSS 386
             A+ E    + +S             R    S V  LS P         E    PE+  
Sbjct: 319 MAAQEERLDKYPSS-----------TGRDEKGSGVYNLSNP--------NEQTGNPEVPP 378

Query: 387 TLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETSLGSRSV 446
             AS   DDGG    S           N+     DD +P  KRRR EG +ME +   + +
Sbjct: 379 ISAS---DDGGEAAAS-----------NRNKDEPDDDDPFSKRRRMEG-AMEITPLVKPI 438

Query: 447 REQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVERASHDL 506
           RE RVVVQ  +E DIL+DGYRWRKYGQKVV+GNPNPRSYYKCT+ GC VRKHVERASHD 
Sbjct: 439 REPRVVVQTLSEVDILDDGYRWRKYGQKVVRGNPNPRSYYKCTAHGCPVRKHVERASHDP 447

BLAST of Carg22727 vs. TAIR 10
Match: AT4G26440.1 (WRKY DNA-binding protein 34 )

HSP 1 Score: 245.0 bits (624), Expect = 1.7e-64
Identity = 153/306 (50.00%), Postives = 186/306 (60.78%), Query Frame = 0

Query: 219 SEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKP 278
           ++DGYNWRKYGQK VKGSEYPRSYYKCTHPNC  KKKVERS +G I EIIYTG H HSKP
Sbjct: 177 ADDGYNWRKYGQKLVKGSEYPRSYYKCTHPNCEAKKKVERSREGHIIEIIYTGDHIHSKP 236

Query: 279 DPTRRAVLGSVLAPDDAPDTGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAV 338
            P RR+ +GS            G G   +++       + Y     + + +E TS VSA 
Sbjct: 237 PPNRRSGIGS-----------SGTGQDMQIDA------TEYEGFAGTNENIEWTSPVSAE 296

Query: 339 TELSEPLYGKTVGGFESIETPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSIS-- 398
            E     YG   G  +            +H    G     +     ++D+ T+  S+S  
Sbjct: 297 LE-----YGSHSGSMQVQN--------GTHQFGYGDAAADALYRDENEDDRTSHMSVSLT 356

Query: 399 --ADDAEPELKRRRKEGISMETSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVK 458
              +  E E KRR+ E  + ETS  +R+ RE RVVVQ  ++ DIL+DGYRWRKYGQKVVK
Sbjct: 357 YDGEVEESESKRRKLEAYATETSGSTRASREPRVVVQTTSDIDILDDGYRWRKYGQKVVK 416

Query: 459 GNPNPRSYYKCTSAGCSVRKHVERASHDLKCVITTYEGKHNHLVPAARNSSSSSSSSSST 518
           GNPNPRSYYKCT+ GC+V KHVERAS D K V+TTY GKH H+VPAARNSS   + SS T
Sbjct: 417 GNPNPRSYYKCTANGCTVTKHVERASDDFKSVLTTYIGKHTHVVPAARNSSHVGAGSSGT 452

Query: 519 AQASSA 521
            Q S A
Sbjct: 477 LQGSLA 452

BLAST of Carg22727 vs. TAIR 10
Match: AT4G26640.1 (WRKY family transcription factor family protein )

HSP 1 Score: 242.3 bits (617), Expect = 1.1e-63
Identity = 157/342 (45.91%), Postives = 187/342 (54.68%), Query Frame = 0

Query: 183 SSVTHNQDIEQPAAPPPPPLETEHKGSYVPVG-----MLKTS-------------EDGYN 242
           SS++         + P PP +     S +P G      ++TS             +DGYN
Sbjct: 84  SSISDAAGSSSELSRPTPPCQMTPTSSDIPAGSDQEESIQTSQNDSRGSTPSILADDGYN 143

Query: 243 WRKYGQKQVKGSEYPRSYYKCTHPNCIVKKKVERSLDGQITEIIYTGAHNHSKPDPTRRA 302
           WRKYGQK VKGSE+PRSYYKCTHPNC VKK  ERS DGQIT+IIY G H+H KP P RR 
Sbjct: 144 WRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITDIIYKGTHDHPKPQPGRR- 203

Query: 303 VLGSVLAPDDAPDTGEGGGSRAKLEGALPWRNSHYGVKDWSVDGLERTSSVSAVTELSEP 362
                            GG  A+ E    + +S             R    S V  LS P
Sbjct: 204 ---------------NSGGMAAQEERLDKYPSS-----------TGRDEKGSGVYNLSNP 263

Query: 363 LYGKTVGGFESIETPELSSTLASHDDDDGGGGGGSGGGGNDDDELTNQGSISADDAEPEL 422
                    E    PE+    AS   DDGG    S           N+     DD +P  
Sbjct: 264 --------NEQTGNPEVPPISAS---DDGGEAAAS-----------NRNKDEPDDDDPFS 323

Query: 423 KRRRKEGISMETSLGSRSVREQRVVVQIETEFDILEDGYRWRKYGQKVVKGNPNPRSYYK 482
           KRRR EG +ME +   + +RE RVVVQ  +E DIL+DGYRWRKYGQKVV+GNPNPRSYYK
Sbjct: 324 KRRRMEG-AMEITPLVKPIREPRVVVQTLSEVDILDDGYRWRKYGQKVVRGNPNPRSYYK 375

Query: 483 CTSAGCSVRKHVERASHDLKCVITTYEGKHNHLVPAARNSSS 507
           CT+ GC VRKHVERASHD K VITTYEGKH+H VP +++SS+
Sbjct: 384 CTAHGCPVRKHVERASHDPKAVITTYEGKHDHDVPTSKSSSN 375

BLAST of Carg22727 vs. TAIR 10
Match: AT3G01080.1 (WRKY DNA-binding protein 58 )

HSP 1 Score: 229.6 bits (584), Expect = 7.3e-60
Identity = 140/313 (44.73%), Postives = 174/313 (55.59%), Query Frame = 0

Query: 201 PLETEHKGSYVPVGMLKTSEDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCIVKKKVERSL 260
           P    +  SY  V + K ++DGYNWRKYGQK +KG EYPRSYYKCTH NC VKKKVERS 
Sbjct: 148 PGNNNNNRSYNVVNVDKPADDGYNWRKYGQKPIKGCEYPRSYYKCTHVNCPVKKKVERSS 207

Query: 261 DGQITEIIYTGAHNHSKPDPTRRAVLGSVLAPDDAPDTGEGGGSRAKLEGALPWRNSHYG 320
           DGQIT+IIY G H+H +P   R                  GGG R   E           
Sbjct: 208 DGQITQIIYKGQHDHERPQNRR------------------GGGGRDSTE----------- 267

Query: 321 VKDWSVDGLERTSSVSAVTELSEPLYGKTVGGF-ESIETPELSSTLASHDDDDGGGGGGS 380
                                        VGG  + +E+ + S     HDDDD       
Sbjct: 268 -----------------------------VGGAGQMMESSDDSGYRKDHDDDD------- 327

Query: 381 GGGGNDDDELTNQGSISADDAEPELKRRRKEGISMETSLGSRSVREQRVVVQIETEFDIL 440
                DDDE         D+  P  K RR +G+S       R+V E +++VQ ++E D+L
Sbjct: 328 -----DDDE--------DDEDLPASKIRRIDGVSTT----HRTVTEPKIIVQTKSEVDLL 378

Query: 441 EDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCSVRKHVERASHDLKCVITTYEGKHNHLVP 500
           +DGYRWRKYGQKVVKGNP+PRSYYKCT+  C+VRKHVERAS D K VITTYEGKHNH VP
Sbjct: 388 DDGYRWRKYGQKVVKGNPHPRSYYKCTTPNCTVRKHVERASTDAKAVITTYEGKHNHDVP 378

Query: 501 AARNSSSSSSSSS 513
           AARN ++++++++
Sbjct: 448 AARNGTAAATAAA 378

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022198.10.0e+00100.00WRKY transcription factor WRKY24, partial [Cucurbita argyrosperma subsp. argyros... [more]
XP_022927490.10.0e+0097.85probable WRKY transcription factor 2 [Cucurbita moschata][more]
KAG6588278.10.0e+0096.64putative WRKY transcription factor 2, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_023003130.10.0e+0093.76WRKY transcription factor SUSIBA2-like [Cucurbita maxima][more]
XP_023520684.10.0e+0093.84WRKY transcription factor WRKY24-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FG771.1e-7639.52Probable WRKY transcription factor 2 OS=Arabidopsis thaliana OX=3702 GN=WRKY2 PE... [more]
Q6B6R47.9e-6752.08WRKY transcription factor WRKY24 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY2... [more]
Q6IEQ76.7e-6651.76WRKY transcription factor WRKY24 OS=Oryza sativa subsp. japonica OX=39947 GN=WRK... [more]
Q93WV02.5e-6538.37Probable WRKY transcription factor 20 OS=Arabidopsis thaliana OX=3702 GN=WRKY20 ... [more]
Q6VWJ67.4e-6538.79WRKY transcription factor SUSIBA2 OS=Hordeum vulgare OX=4513 GN=WRKY46 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EP380.0e+0097.85probable WRKY transcription factor 2 OS=Cucurbita moschata OX=3662 GN=LOC1114342... [more]
A0A6J1KQX20.0e+0093.76WRKY transcription factor SUSIBA2-like OS=Cucurbita maxima OX=3661 GN=LOC1114968... [more]
A0A6J1HD236.6e-21859.50WRKY transcription factor SUSIBA2-like isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1JK301.1e-21759.37WRKY transcription factor WRKY24-like isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A0A0M0L17.6e-20658.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G657480 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G56270.17.8e-7839.52WRKY DNA-binding protein 2 [more]
AT4G26640.21.8e-6638.37WRKY family transcription factor family protein [more]
AT4G26440.11.7e-6450.00WRKY DNA-binding protein 34 [more]
AT4G26640.11.1e-6345.91WRKY family transcription factor family protein [more]
AT3G01080.17.3e-6044.73WRKY DNA-binding protein 58 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 439..498
e-value: 2.0E-36
score: 137.0
coord: 219..277
e-value: 6.1E-36
score: 135.4
IPR003657WRKY domainPFAMPF03106WRKYcoord: 440..496
e-value: 5.4E-24
score: 84.2
coord: 220..275
e-value: 1.1E-24
score: 86.4
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 434..499
score: 36.781685
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 214..278
score: 23.43347
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 214..279
e-value: 2.6E-27
score: 96.4
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 426..492
e-value: 4.4E-34
score: 118.0
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 217..278
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 433..499
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 399..414
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 499..539
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 290..309
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 361..423
NoneNo IPR availablePANTHERPTHR31221:SF123WRKY TRANSCRIPTION FACTOR SUSIBA2-LIKE ISOFORM X1coord: 43..620
IPR044810WRKY transcription factor, plantPANTHERPTHR31221WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATEDcoord: 43..620

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22727-RACarg22727-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding