Carg22725 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22725
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	4-hydroxy-4-methyl-2-oxoglutarate aldolase
Location	Carg_Chr11: 5028755 .. 5030652 (+)
RNA-Seq Expression	Carg22725
Synteny	Carg22725

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTTAAGTGATTTGACATGGACAAAAGTGGAGAATGAAAGACTCGAAAGGGATCAGGTTTCTCATATCATATATAAAACATTGATTCTAGCTCCCACCCGTGGAGAATCAGGAAGAATCTAGGCTTGTGACAGCTTTAGTGCTGTTTGATAGAATTTATGGCTTAAGAGACATAAAATAATCTTTAATGATTTGCACAAAAGTTTTGGTCTACTTCAGGAAGTCATAATTTTGTTCCTTCTTCTTAGTTCCTTGTAACGGCTACCCTGCTTCTTTGTGTCCGGGTTGTAGAATCTTATAAGTTGAGGAATGTCGCAATGAGAGAATTATCTTATCGAGTATGTTGATGAGTGGGATTAGTGTGAGATCCCACGTCAATTGAATAGGAGAATGAAACATTCCTTATAAGGGCGTGTAAACCTCTCTCTATAAAATACGTTTTAAAACCTCAAGGAGAAATTCGAAAGGGAAAGTCCAAAGAAGACAATATCTACTAGCAGTGAGCTTGAACGGTTACAATTATTCATATTTAAATGTTCTAGTTTTTGAAATAGTATAGAAAATTTGAACTTTTAACTGTGTTTCTGGTAACTCGTGAGCTCCATTTTACAAGAACCGAACAACAAATACAAGAATGACATGGATAAACCCTTGATCTTGTCATTGACCTTTGTGATCAGGAGCCTTCAAATAAGTGTGCTATCTTTTCCAAGAGGATATTGCACATTGTTTTGCTATGGCTCAAAGTTGGTTGTGATGATCTGCTTTCTACTGCACACGAACATATAAGCCTTTTGTTTTTACTTGCTTCTGATAGTGCTTTTATCAGCAAAGTCCTTGTACTTGTGTTTTCTTTCAAGAAATGACCAAATAGCAGAATCTCTGGTTCACTGAATTCAATATTTGGTTACAAACACAAGGTGCTGGAGCAAATGGCCTTGGTTACCACTGCAGAAGTTTGTGATGCTAATCCACAGCTCATTGTGAGTGGTGAACTCCGAGCCCTGGAGCCAATCTTCCAGATATATGGGCGACGCCAAGTGTTTTCAGGACCAGTAGTGACACTAAAGGTGTTTGAAGACAATGTGTTGGTACGTGAGTTCCTGGAGGAGAAGGGTAAGGGCAGAGTTCTTGTGGTGGACGGAGGCGGTAGCAAGAGGTGTGCAATATTGGGTGGCAATCCTGTTGTCCAAGCTCAGAACAACGGATGGGCTGGCATAGTTGTGAATGGTTGTGTCCGAGACGTGGATGAAATCAATGGGTGCGATATTGGGGTCAGGGCTCTGGCTTCACATCCAATGAAGGCCAACAAGAAGCGGGTTGGAGAGAAGCACGTCCCTATAACTGTGGGCGGGACAAGGATCATGGATGGGGAGTGGCTGTATGCTGATACCGATGGGATTCTGGTTTCACGGACTGAGTTGTCTGTTTAGAGAAGTGTTGTTCAAAGATTTTGATGGCAGGATGATATTGTTAGACAAGAGCATGAGCAAGAGCTTGTCAGTAATGGTGTTGATGTCTATTATATTTATCATTAAGATTATGTTATGTGGTTTGAATGATGAACTGCTGCGTTATGTGGCCTTTATTGTTGTTGTATGCAACATGTTAATGAAAAACTTGATCATTTTGAATCTTTGTTTTGTCTATAAAACTGATTTCAGGTGGGCGATTCTTCTAAAAGATGGCTTTTGGGTTGCAGCTATGTGTTACGGTCGTACTATTTGAATCGTGCATCATGGTGATCTTGATACTATCATGACAGACAAGGTTTAAGGAGAACAAAAGTAGACTGTGCACTATACAAAGGCAAGTAAGCACTTTCTGTGATTTGTAATTTACTCGTTGTTTGACAGCTAAAAGAGGGTCTTCTGGGTTGCAGCTATGTGTCGCAGTCG

mRNA sequence

GTTTTAAGTGATTTGACATGGACAAAAGTGGAGAATGAAAGACTCGAAAGGGATCAGGTGCTGGAGCAAATGGCCTTGGTTACCACTGCAGAAGTTTGTGATGCTAATCCACAGCTCATTGTGAGTGGTGAACTCCGAGCCCTGGAGCCAATCTTCCAGATATATGGGCGACGCCAAGTGTTTTCAGGACCAGTAGTGACACTAAAGGTGTTTGAAGACAATGTGTTGGTACGTGAGTTCCTGGAGGAGAAGGGTAAGGGCAGAGTTCTTGTGGTGGACGGAGGCGGTAGCAAGAGGTGTGCAATATTGGGTGGCAATCCTGTTGTCCAAGCTCAGAACAACGGATGGGCTGGCATAGTTGTGAATGGTTGTGTCCGAGACGTGGATGAAATCAATGGGTGCGATATTGGGGTCAGGGCTCTGGCTTCACATCCAATGAAGGCCAACAAGAAGCGGGTTGGAGAGAAGCACGTCCCTATAACTGTGGGCGGGACAAGGATCATGGATGGGGAGTGGCTGTATGCTGATACCGATGGGATTCTGGTTTCACGGACTGAGTTGTCTGTTTAGAGAAGTGTTGTTCAAAGATTTTGATGGCAGGATGATATTGTTAGACAAGAGCATGAGCAAGAGCTTGTCAGTAATGGTGTTGATGTCTATTATATTTATCATTAAGATTATGTTATGTGGTTTGAATGATGAACTGCTGCGTTATGTGGCCTTTATTGTTGTTGTATGCAACATGTTAATGAAAAACTTGATCATTTTGAATCTTTGTTTTGTCTATAAAACTGATTTCAGGTGGGCGATTCTTCTAAAAGATGGCTTTTGGGTTGCAGCTATGTGTTACGGTCGTACTATTTGAATCGTGCATCATGGTGATCTTGATACTATCATGACAGACAAGGTTTAAGGAGAACAAAAGTAGACTGTGCACTATACAAAGGCAAGTAAGCACTTTCTGTGATTTGTAATTTACTCGTTGTTTGACAGCTAAAAGAGGGTCTTCTGGGTTGCAGCTATGTGTCGCAGTCG

Coding sequence (CDS)

GTTTTAAGTGATTTGACATGGACAAAAGTGGAGAATGAAAGACTCGAAAGGGATCAGGTGCTGGAGCAAATGGCCTTGGTTACCACTGCAGAAGTTTGTGATGCTAATCCACAGCTCATTGTGAGTGGTGAACTCCGAGCCCTGGAGCCAATCTTCCAGATATATGGGCGACGCCAAGTGTTTTCAGGACCAGTAGTGACACTAAAGGTGTTTGAAGACAATGTGTTGGTACGTGAGTTCCTGGAGGAGAAGGGTAAGGGCAGAGTTCTTGTGGTGGACGGAGGCGGTAGCAAGAGGTGTGCAATATTGGGTGGCAATCCTGTTGTCCAAGCTCAGAACAACGGATGGGCTGGCATAGTTGTGAATGGTTGTGTCCGAGACGTGGATGAAATCAATGGGTGCGATATTGGGGTCAGGGCTCTGGCTTCACATCCAATGAAGGCCAACAAGAAGCGGGTTGGAGAGAAGCACGTCCCTATAACTGTGGGCGGGACAAGGATCATGGATGGGGAGTGGCTGTATGCTGATACCGATGGGATTCTGGTTTCACGGACTGAGTTGTCTGTTTAG

Protein sequence

VLSDLTWTKVENERLERDQVLEQMALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEEKGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALASHPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV

Homology

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BLAST of Carg22725 vs. NCBI nr
Match: KAG7022196.1 (putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 380.6 bits (976), Expect = 8.1e-102
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0


Query: 1   VLSDLTWTKVENERLERDQVLEQMALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQV 60
           VLSDLTWTKVENERLERDQVLEQMALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQV
Sbjct: 1   VLSDLTWTKVENERLERDQVLEQMALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQV 60

Query: 61  FSGPVVTLKVFEDNVLVREFLEEKGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIV 120
           FSGPVVTLKVFEDNVLVREFLEEKGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIV
Sbjct: 61  FSGPVVTLKVFEDNVLVREFLEEKGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIV 120

Query: 121 VNGCVRDVDEINGCDIGVRALASHPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGI 180
           VNGCVRDVDEINGCDIGVRALASHPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGI
Sbjct: 121 VNGCVRDVDEINGCDIGVRALASHPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGI 180

Query: 181 LVSRTELSV 190
           LVSRTELSV
Sbjct: 181 LVSRTELSV 189

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BLAST of Carg22725 vs. NCBI nr
Match: KAG6588276.1 (Isopentenyl-diphosphate Delta-isomerase I, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 343.6 bits (880), Expect = 1.1e-90
Identity = 170/171 (99.42%), Postives = 171/171 (100.00%), Query Frame = 0


Query: 19  QVLEQMALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVR 78
           +VLEQMALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVR
Sbjct: 276 KVLEQMALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVR 335

Query: 79  EFLEEKGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGV 138
           EFLEEKGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGV
Sbjct: 336 EFLEEKGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGV 395

Query: 139 RALASHPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           RALASHPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 396 RALASHPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 446

[Close]

BLAST of Carg22725 vs. NCBI nr
Match: XP_022933782.1 (putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita moschata] >XP_023005764.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita maxima] >XP_023520656.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 336.7 bits (862), Expect = 1.3e-88
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg22725 vs. NCBI nr
Match: XP_008443692.1 (PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis melo] >XP_038880156.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Benincasa hispida] >KAA0038259.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis melo var. makuwa] >TYK30658.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 328.9 bits (842), Expect = 2.8e-86
Identity = 162/166 (97.59%), Postives = 163/166 (98.19%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVL+REFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG GRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HPMKA KKRVGEKHVPITV GTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg22725 vs. NCBI nr
Match: XP_022988625.1 (putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita maxima] >XP_022988626.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita maxima])

HSP 1 Score: 328.2 bits (840), Expect = 4.8e-86
Identity = 160/166 (96.39%), Postives = 163/166 (98.19%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MALVTTAEVCDANPQLIVSGELRALEP+FQIYGRRQVFSGPVVTLKVFEDNVL+REFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG GRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HPMKA KKRVGEKHVPIT+ GTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg22725 vs. ExPASy Swiss-Prot
Match: Q9FFE0 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 OS=Arabidopsis thaliana OX=3702 GN=At5g16450 PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 3.8e-78
Identity = 138/166 (83.13%), Postives = 155/166 (93.37%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MA VTTAEVCDAN +LI SG+LRAL+PIFQIYGRRQ+FSGPVVT+KVFEDN L+R+F+EE
Sbjct: 1   MAFVTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG GRVLVVDGGGS+RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HP+KA+KK +GE+ VP+ + GTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Carg22725 vs. ExPASy Swiss-Prot
Match: Q9M8R9 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g02770 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 2.7e-76
Identity = 135/166 (81.33%), Postives = 152/166 (91.57%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MA VTTAEVCDAN ++I SG+LRAL+P+FQIYGRRQ+FSGPVVT+KVFEDN L+R FLEE
Sbjct: 1   MAFVTTAEVCDANQEMIRSGQLRALQPVFQIYGRRQIFSGPVVTVKVFEDNGLIRHFLEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG GRVLVVDGGGS RCAILGGNPVVQAQNNGWAGI+VNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HP+KA+KK +GE+ V + + GTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVSLNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Carg22725 vs. ExPASy Swiss-Prot
Match: Q9FH13 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 OS=Arabidopsis thaliana OX=3702 GN=At5g56260 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 2.8e-65
Identity = 118/166 (71.08%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MA   TAE CD+N +LI +G+LRAL PIF+IYG+R+ FSGP+VTLKVFEDNVLVR  LE 
Sbjct: 1   MAAFATAEACDSNAELISNGDLRALHPIFKIYGQRRCFSGPIVTLKVFEDNVLVRNQLET 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG+G VLV+DGGGS RCA++GGN    AQNNGW+GIVVNGCVRDVDEIN CD+GVRAL S
Sbjct: 61  KGEGGVLVIDGGGSMRCALVGGNLGQLAQNNGWSGIVVNGCVRDVDEINDCDVGVRALGS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           +P+K+ KK  GEK+VP+ +GGT I DGEWLYAD+DGIL+S+TELSV
Sbjct: 121 NPLKSTKKGHGEKNVPVHIGGTLIRDGEWLYADSDGILISKTELSV 166

[Close]

BLAST of Carg22725 vs. ExPASy Swiss-Prot
Match: Q46ZV6 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=Reut_A1963 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 2.2e-49
Identity = 86/159 (54.09%), Postives = 116/159 (72.96%), Query Frame = 0


Query: 29  TAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEEKGKGR 88
           T ++CDAN   +  G LR + P+F+ +G++Q F+GP  TLK+FEDN LVRE LE  G+GR
Sbjct: 5   TTDLCDANEPRLADGTLRVMAPVFRAFGKQQAFAGPAATLKIFEDNSLVREALESPGQGR 64

Query: 89  VLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALASHPMKA 148
           VLVVDGGGS RCA++GGN  + A+ NGW GI+VNGCVRD  E++ CDIG+RALA+HP K+
Sbjct: 65  VLVVDGGGSLRCALVGGNLGLLAEKNGWVGIIVNGCVRDTGELDVCDIGIRALAAHPQKS 124

Query: 149 NKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTEL 188
            K+  GE+ V + + G  +  G W+Y D DG+LVS  +L
Sbjct: 125 QKRGAGERDVAVQMPGAVVRPGNWIYVDADGVLVSEEKL 163

[Close]

BLAST of Carg22725 vs. ExPASy Swiss-Prot
Match: A9AHQ6 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=Bmul_1223 PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 8.2e-49
Identity = 92/160 (57.50%), Postives = 116/160 (72.50%), Query Frame = 0


Query: 29  TAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEEKGKGR 88
           T ++CDA+   + +G LR L+P  + YG    F+GP  TLKVFEDN LVR  LE+ G GR
Sbjct: 5   TTDLCDAHEDKLAAGTLRVLQPALRPYGGAARFAGPAATLKVFEDNTLVRAALEQDGGGR 64

Query: 89  VLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALASHPMKA 148
           VLVVDGG S RCA++GGN    A+ NGWAGIVV+GCVRD  E+  C +GV ALA+HP K+
Sbjct: 65  VLVVDGGASLRCALVGGNLGALAEKNGWAGIVVHGCVRDAAELRECKVGVLALATHPRKS 124

Query: 149 NKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELS 189
           +K+  GE+ VP+TV GTRI  GEW+YAD DG+LVS T L+
Sbjct: 125 DKRGAGERDVPVTVLGTRIAPGEWIYADDDGVLVSATSLT 164

[Close]

BLAST of Carg22725 vs. ExPASy TrEMBL
Match: A0A6J1L070 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita maxima OX=3661 GN=LOC111498662 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 6.5e-89
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg22725 vs. ExPASy TrEMBL
Match: A0A6J1F0S6 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita moschata OX=3662 GN=LOC111441092 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 6.5e-89
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg22725 vs. ExPASy TrEMBL
Match: A0A5D3E4B6 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00850 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.4e-86
Identity = 162/166 (97.59%), Postives = 163/166 (98.19%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVL+REFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG GRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HPMKA KKRVGEKHVPITV GTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg22725 vs. ExPASy TrEMBL
Match: A0A1S3B858 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo OX=3656 GN=LOC103487227 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.4e-86
Identity = 162/166 (97.59%), Postives = 163/166 (98.19%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVL+REFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG GRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HPMKA KKRVGEKHVPITV GTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITVAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg22725 vs. ExPASy TrEMBL
Match: A0A6J1JHR3 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita maxima OX=3661 GN=LOC111485820 PE=3 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.3e-86
Identity = 160/166 (96.39%), Postives = 163/166 (98.19%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MALVTTAEVCDANPQLIVSGELRALEP+FQIYGRRQVFSGPVVTLKVFEDNVL+REFLEE
Sbjct: 1   MALVTTAEVCDANPQLIVSGELRALEPVFQIYGRRQVFSGPVVTLKVFEDNVLIREFLEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG GRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HPMKA KKRVGEKHVPIT+ GTRIMDGEWLYADTDGILVSRTELSV
Sbjct: 121 HPMKAYKKRVGEKHVPITIAGTRIMDGEWLYADTDGILVSRTELSV 166

[Close]

BLAST of Carg22725 vs. TAIR 10
Match: AT5G16450.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 292.4 bits (747), Expect = 2.7e-79
Identity = 138/166 (83.13%), Postives = 155/166 (93.37%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MA VTTAEVCDAN +LI SG+LRAL+PIFQIYGRRQ+FSGPVVT+KVFEDN L+R+F+EE
Sbjct: 1   MAFVTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG GRVLVVDGGGS+RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HP+KA+KK +GE+ VP+ + GTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Carg22725 vs. TAIR 10
Match: AT5G16450.2 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 292.4 bits (747), Expect = 2.7e-79
Identity = 138/166 (83.13%), Postives = 155/166 (93.37%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MA VTTAEVCDAN +LI SG+LRAL+PIFQIYGRRQ+FSGPVVT+KVFEDN L+R+F+EE
Sbjct: 1   MAFVTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG GRVLVVDGGGS+RCAILGGNPVVQAQNNGWAGIVVNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HP+KA+KK +GE+ VP+ + GTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Carg22725 vs. TAIR 10
Match: AT3G02770.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 286.2 bits (731), Expect = 1.9e-77
Identity = 135/166 (81.33%), Postives = 152/166 (91.57%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MA VTTAEVCDAN ++I SG+LRAL+P+FQIYGRRQ+FSGPVVT+KVFEDN L+R FLEE
Sbjct: 1   MAFVTTAEVCDANQEMIRSGQLRALQPVFQIYGRRQIFSGPVVTVKVFEDNGLIRHFLEE 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG GRVLVVDGGGS RCAILGGNPVVQAQNNGWAGI+VNGC+RDVDEINGCDIGVRALAS
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALAS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           HP+KA+KK +GE+ V + + GTRI DGEWLYADTDGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVSLNIAGTRICDGEWLYADTDGILVSQIELSV 166

[Close]

BLAST of Carg22725 vs. TAIR 10
Match: AT5G56260.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 249.6 bits (636), Expect = 2.0e-66
Identity = 118/166 (71.08%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 24  MALVTTAEVCDANPQLIVSGELRALEPIFQIYGRRQVFSGPVVTLKVFEDNVLVREFLEE 83
           MA   TAE CD+N +LI +G+LRAL PIF+IYG+R+ FSGP+VTLKVFEDNVLVR  LE 
Sbjct: 1   MAAFATAEACDSNAELISNGDLRALHPIFKIYGQRRCFSGPIVTLKVFEDNVLVRNQLET 60

Query: 84  KGKGRVLVVDGGGSKRCAILGGNPVVQAQNNGWAGIVVNGCVRDVDEINGCDIGVRALAS 143
           KG+G VLV+DGGGS RCA++GGN    AQNNGW+GIVVNGCVRDVDEIN CD+GVRAL S
Sbjct: 61  KGEGGVLVIDGGGSMRCALVGGNLGQLAQNNGWSGIVVNGCVRDVDEINDCDVGVRALGS 120

Query: 144 HPMKANKKRVGEKHVPITVGGTRIMDGEWLYADTDGILVSRTELSV 190
           +P+K+ KK  GEK+VP+ +GGT I DGEWLYAD+DGIL+S+TELSV
Sbjct: 121 NPLKSTKKGHGEKNVPVHIGGTLIRDGEWLYADSDGILISKTELSV 166

The following BLAST results are available for this feature:

BLAST of Carg22725 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022196.1	8.1e-102	100.00	putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2, partial [Cucurbita argyro...	[more]
KAG6588276.1	1.1e-90	99.42	Isopentenyl-diphosphate Delta-isomerase I, partial [Cucurbita argyrosperma subsp...	[more]
XP_022933782.1	1.3e-88	100.00	putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita moschata] >XP_0...	[more]
XP_008443692.1	2.8e-86	97.59	PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucumis melo] ...	[more]
XP_022988625.1	4.8e-86	96.39	putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 [Cucurbita maxima] >XP_022...	[more]

BLAST of Carg22725 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FFE0	3.8e-78	83.13	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 OS=Arabidopsis thaliana OX...	[more]
Q9M8R9	2.7e-76	81.33	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 1 OS=Arabidopsis thaliana OX...	[more]
Q9FH13	2.8e-65	71.08	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 OS=Arabidopsis thaliana OX...	[more]
Q46ZV6	2.2e-49	54.09	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cupriavidus pinatubonensi...	[more]
A9AHQ6	8.2e-49	57.50	Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Burkholderia multivorans ...	[more]

BLAST of Carg22725 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L070	6.5e-89	100.00	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1F0S6	6.5e-89	100.00	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A5D3E4B6	1.4e-86	97.59	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3B858	1.4e-86	97.59	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo OX=3656 GN=LOC1034872...	[more]
A0A6J1JHR3	2.3e-86	96.39	4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of Carg22725 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16450.1	2.7e-79	83.13	Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase	[more]
AT5G16450.2	2.7e-79	83.13	Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase	[more]
AT3G02770.1	1.9e-77	81.33	Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase	[more]
AT5G56260.1	2.0e-66	71.08	Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005493	Ribonuclease E inhibitor RraA/RraA-like protein	PFAM	PF03737	RraA-like	coord: 29..182 e-value: 1.3E-42 score: 145.4
IPR005493	Ribonuclease E inhibitor RraA/RraA-like protein	CDD	cd16841	RraA_family	coord: 30..183 e-value: 1.72172E-55 score: 170.718
None	No IPR available	GENE3D	3.50.30.40		coord: 26..189 e-value: 5.6E-49 score: 167.9
None	No IPR available	PANTHER	PTHR33254	4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED	coord: 27..189
None	No IPR available	PANTHER	PTHR33254:SF20	4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE	coord: 27..189
IPR010203	Regulator of ribonuclease activity A	TIGRFAM	TIGR01935	TIGR01935	coord: 29..183 e-value: 6.6E-65 score: 215.5
IPR036704	Ribonuclease E inhibitor RraA/RraA-like superfamily	SUPERFAMILY	89562	RraA-like	coord: 27..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg22725-RA	Carg22725-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0051252	regulation of RNA metabolic process
molecular_function	GO:0047443	4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008948	oxaloacetate decarboxylase activity
molecular_function	GO:0008428	ribonuclease inhibitor activity