Carg22712 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22712
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRRM domain-containing protein
LocationCarg_Chr11: 4877124 .. 4879829 (+)
RNA-Seq ExpressionCarg22712
SyntenyCarg22712
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAAGTTGGGTCTTTCCCATATTTCAGAGGGGTCCCTGTTGGGCGACTGCTGGGTCTGCGAGCTCGAAGAAGAAGAAGAAGAAAAGCCATGGAAGAAGGAGAAAGCGCCTCCACGAAGATGAGGATTTACGTTGGAGGACTAGGTGCTGCCATGACTGAAGACGACCTCAGGAAGGTTTTTCAGAGCGTCGGTGGCGTGGTGGAGGCCGTTGATTTCATTCGTTCCAAATCTCGGAGCTTCGCTTACGTCGACTTCTTTCCGTCCTCCCAATCGTCCGTCTCCAAACTCTTCAGCACTGTAAGTTTTTTCTCCTCGATTCCATTGCCGAACTCTCATTACGTTGTTCCCCCCCCCCCCCACCGATTTTGGCTATGTTTCTCTTGAATGTGTGTTTATATGCCTATCTCGCTATCTGGTTGCGATTTTAACCTAATTGTAGTTCGATTATTGCATTTTCCGTACCTTTTCATGAATTAGATAGCGCGTTCCTAATTTAGTTGCTGAAAAGTTCGTTATTCTTGTACATAGTACAATGGATGTGCTTGGAAAGGAGGAAAGTTGAGGCTTGAGAAAGCTAAGGAACATTATCTTGCTCGCTTGAGGCGGGAGTGGGAGGAAGATGCTGAAATTATGAATTATGATGATGGTGCAGACCTGGAGACTTATGCTCCAGAATCTACAGAGCATGTTGCGAAATCAGAGCACATTCAGATTTTCTTTCCAAGTTTAGGAGAGGTGATTCTTTGCTTACGAAGTTTTCATTTTGAGATGATTCAATCAATGACTGTTCTTTAGATTCTTAAGTATGAATTTTTTTTATAGGTGAAGTCTTTACCAATTAGTGGTACGGGAACTCACAAATATGATTTTCCACATGTTGAGGTGCCTCCTCTTCCTGTGCATTTTTGTGACTGTGAAGAGCATAATGTTTCTGATCCCACTAGCAAATCCATGGACGCAAAAACAGGAGATTTGGATGCTGGGAATGGTGGAATTGGTGAAGATGAAATCAAGATGATGAATACAGTGTTGAACAAGCTCTTTGAGAGGCAGGAAGCTTCTCATGCTAATTGTAATGGAACCATGGGAGTCAAGGATAAAGACAACTCTAAGATATTGACCGATAATCAACCACTTGAAGATAATAAAGAGGATAGCGATGAAGATAGCCTTGTGCTTAATGTAATGGCTAGTGGCTCCAATTCCAAACCTTTGCCATTGAACAGTGGCAGTAAAAGTTTCAAAGCTCATGGGAACAGTAAGGTTAAGAATTACGCTATCACCATCGCCATTTTATCGTTTTTTCCTTCACGTTTTAAGATATATTTGAGAGGGTATGTTTATCTCAAGGGTTTGTTGAAGCATTTTGGGTTTTTTGTATTTCATTCGGTTTTTATCATCTTGAACTTTGCGGTCGAGGTATACTTATAAAAATGGATCGTTTCAGGGTGCGGACAGGGATCAGAAAGGTAATAGCAGAGTTCAAAGCAAGAAAAGGAAATCGGTTACCAATGAGGAATTTGATAGTAATGAATATGTGCCCAATATCTCTACTGGCAGTGGAAAGGGGAACACAAATCCAGCATATGAGCCAGTTGGACCCTCGAGGCCTCAAGCTCCTGATCGAGCAATGCCGATCCAATCTTCACGTTCTCAGAAATCTTCATGGAAAACACTTATTTGTGATAAGAGCAAGGCTTCATTCAGCATCTCAGACATACTGCCTTCTGTTCCTTCAGCAAATGAAGAGCAACCAGAAGCAGATGCTCTAAGTCTAGCTCATTCATCTCCGAACAGAAACAGCGACCGTGCTACTGCTGCAGTATTGAAAAGAAAAAAAGATAAAACGAAACCCGCGAATAGCAATGTTTCCTTCAGCATTTTAGACACACTGCCTACTGCGTCTTCAGGAGATCAAGAGCAAACAGAAGCTGATGATCCGAATCGAGCTCATTCAACTCCAAACAGAAACAGCGACCTTGCTACTGCTGCAGTATTGAAAAGGAAAAAAGATGAAACGAAACCCGCGAATAGCAATGTTTCGTTCTGCATTTCAGACGCACTGCCTACTGCATCATCAGCAGATCAAAAGCAAACAGAAGCCGAGGATCTGAATCTAGCTGCAACTGATGCAATATTTGAAAGCAAATCAAAAGAAATGAAATCTGTGGAGAGCTCCCCAGAAGCCGAAAATACTGTTCGAAATGTCACCTCGAATCAAGGAAGAGGCGCTGCATGGAAGCAAAAATCTTCATGGACACAATTAGTTAGCCAGGAAGCCACCTCCTTCAGTATTACTCAAATTTTACCAAACAATACTTCTGAAAAGCAGGTACAAAGGGAATCCGATGTCATCAATGTTAATCTTTTCGCGCCAAGCGAAAATAAGGATTCAATAGAACAGCAAATTCAAAGTACTGCGGCAGATGATTCTGCTGCATTTGTAGTTGCAAAAGATGAAACTGCCTGTTATGATGTCAAGAAGAACGACCAACCTGCAGTGCAAGAAAATGAGCCCTCTCCCACCGAAGCTATTGAGAGGCATATTAAACCACAGGAAGCAGGTTCATTTGATGCTAAAAGCGGCGAAACTTGCTGCCCGTTTATGAGAAATTCTCGGTCGGTAGCCGAGTGGACAAAGATAAAAGCTGCTCTTTCTGGTGGATCCAAGAAGAAAAAGCAGAGACAATTGGACACTCAATAG

mRNA sequence

GTAAAGTTGGGTCTTTCCCATATTTCAGAGGGGTCCCTGTTGGGCGACTGCTGGGTCTGCGAGCTCGAAGAAGAAGAAGAAGAAAAGCCATGGAAGAAGGAGAAAGCGCCTCCACGAAGATGAGGATTTACGTTGGAGGACTAGGTGCTGCCATGACTGAAGACGACCTCAGGAAGGTTTTTCAGAGCGTCGGTGGCGTGGTGGAGGCCGTTGATTTCATTCGTTCCAAATCTCGGAGCTTCGCTTACGTCGACTTCTTTCCGTCCTCCCAATCGTCCGTCTCCAAACTCTTCAGCACTTACAATGGATGTGCTTGGAAAGGAGGAAAGTTGAGGCTTGAGAAAGCTAAGGAACATTATCTTGCTCGCTTGAGGCGGGAGTGGGAGGAAGATGCTGAAATTATGAATTATGATGATGGTGCAGACCTGGAGACTTATGCTCCAGAATCTACAGAGCATGTTGCGAAATCAGAGCACATTCAGATTTTCTTTCCAAGTTTAGGAGAGGTGAAGTCTTTACCAATTAGTGGTACGGGAACTCACAAATATGATTTTCCACATGTTGAGGTGCCTCCTCTTCCTGTGCATTTTTGTGACTGTGAAGAGCATAATGTTTCTGATCCCACTAGCAAATCCATGGACGCAAAAACAGGAGATTTGGATGCTGGGAATGGTGGAATTGGTGAAGATGAAATCAAGATGATGAATACAGTGTTGAACAAGCTCTTTGAGAGGCAGGAAGCTTCTCATGCTAATTGTAATGGAACCATGGGAGTCAAGGATAAAGACAACTCTAAGATATTGACCGATAATCAACCACTTGAAGATAATAAAGAGGATAGCGATGAAGATAGCCTTGTGCTTAATGTAATGGCTAGTGGCTCCAATTCCAAACCTTTGCCATTGAACAGTGGCAGTAAAAGTTTCAAAGCTCATGGGAACAGTAAGGGTGCGGACAGGGATCAGAAAGGTAATAGCAGAGTTCAAAGCAAGAAAAGGAAATCGGTTACCAATGAGGAATTTGATAGTAATGAATATGTGCCCAATATCTCTACTGGCAGTGGAAAGGGGAACACAAATCCAGCATATGAGCCAGTTGGACCCTCGAGGCCTCAAGCTCCTGATCGAGCAATGCCGATCCAATCTTCACGTTCTCAGAAATCTTCATGGAAAACACTTATTTGTGATAAGAGCAAGGCTTCATTCAGCATCTCAGACATACTGCCTTCTGTTCCTTCAGCAAATGAAGAGCAACCAGAAGCAGATGCTCTAAGTCTAGCTCATTCATCTCCGAACAGAAACAGCGACCGTGCTACTGCTGCAGTATTGAAAAGAAAAAAAGATAAAACGAAACCCGCGAATAGCAATGTTTCCTTCAGCATTTTAGACACACTGCCTACTGCGTCTTCAGGAGATCAAGAGCAAACAGAAGCTGATGATCCGAATCGAGCTCATTCAACTCCAAACAGAAACAGCGACCTTGCTACTGCTGCAGTATTGAAAAGGAAAAAAGATGAAACGAAACCCGCGAATAGCAATGTTTCGTTCTGCATTTCAGACGCACTGCCTACTGCATCATCAGCAGATCAAAAGCAAACAGAAGCCGAGGATCTGAATCTAGCTGCAACTGATGCAATATTTGAAAGCAAATCAAAAGAAATGAAATCTGTGGAGAGCTCCCCAGAAGCCGAAAATACTGTTCGAAATGTCACCTCGAATCAAGGAAGAGGCGCTGCATGGAAGCAAAAATCTTCATGGACACAATTAGTTAGCCAGGAAGCCACCTCCTTCAGTATTACTCAAATTTTACCAAACAATACTTCTGAAAAGCAGGTACAAAGGGAATCCGATGTCATCAATGTTAATCTTTTCGCGCCAAGCGAAAATAAGGATTCAATAGAACAGCAAATTCAAAGTACTGCGGCAGATGATTCTGCTGCATTTGTAGTTGCAAAAGATGAAACTGCCTGTTATGATGTCAAGAAGAACGACCAACCTGCAGTGCAAGAAAATGAGCCCTCTCCCACCGAAGCTATTGAGAGGCATATTAAACCACAGGAAGCAGGTTCATTTGATGCTAAAAGCGGCGAAACTTGCTGCCCGTTTATGAGAAATTCTCGGTCGGTAGCCGAGTGGACAAAGATAAAAGCTGCTCTTTCTGGTGGATCCAAGAAGAAAAAGCAGAGACAATTGGACACTCAATAG

Coding sequence (CDS)

ATGGAAGAAGGAGAAAGCGCCTCCACGAAGATGAGGATTTACGTTGGAGGACTAGGTGCTGCCATGACTGAAGACGACCTCAGGAAGGTTTTTCAGAGCGTCGGTGGCGTGGTGGAGGCCGTTGATTTCATTCGTTCCAAATCTCGGAGCTTCGCTTACGTCGACTTCTTTCCGTCCTCCCAATCGTCCGTCTCCAAACTCTTCAGCACTTACAATGGATGTGCTTGGAAAGGAGGAAAGTTGAGGCTTGAGAAAGCTAAGGAACATTATCTTGCTCGCTTGAGGCGGGAGTGGGAGGAAGATGCTGAAATTATGAATTATGATGATGGTGCAGACCTGGAGACTTATGCTCCAGAATCTACAGAGCATGTTGCGAAATCAGAGCACATTCAGATTTTCTTTCCAAGTTTAGGAGAGGTGAAGTCTTTACCAATTAGTGGTACGGGAACTCACAAATATGATTTTCCACATGTTGAGGTGCCTCCTCTTCCTGTGCATTTTTGTGACTGTGAAGAGCATAATGTTTCTGATCCCACTAGCAAATCCATGGACGCAAAAACAGGAGATTTGGATGCTGGGAATGGTGGAATTGGTGAAGATGAAATCAAGATGATGAATACAGTGTTGAACAAGCTCTTTGAGAGGCAGGAAGCTTCTCATGCTAATTGTAATGGAACCATGGGAGTCAAGGATAAAGACAACTCTAAGATATTGACCGATAATCAACCACTTGAAGATAATAAAGAGGATAGCGATGAAGATAGCCTTGTGCTTAATGTAATGGCTAGTGGCTCCAATTCCAAACCTTTGCCATTGAACAGTGGCAGTAAAAGTTTCAAAGCTCATGGGAACAGTAAGGGTGCGGACAGGGATCAGAAAGGTAATAGCAGAGTTCAAAGCAAGAAAAGGAAATCGGTTACCAATGAGGAATTTGATAGTAATGAATATGTGCCCAATATCTCTACTGGCAGTGGAAAGGGGAACACAAATCCAGCATATGAGCCAGTTGGACCCTCGAGGCCTCAAGCTCCTGATCGAGCAATGCCGATCCAATCTTCACGTTCTCAGAAATCTTCATGGAAAACACTTATTTGTGATAAGAGCAAGGCTTCATTCAGCATCTCAGACATACTGCCTTCTGTTCCTTCAGCAAATGAAGAGCAACCAGAAGCAGATGCTCTAAGTCTAGCTCATTCATCTCCGAACAGAAACAGCGACCGTGCTACTGCTGCAGTATTGAAAAGAAAAAAAGATAAAACGAAACCCGCGAATAGCAATGTTTCCTTCAGCATTTTAGACACACTGCCTACTGCGTCTTCAGGAGATCAAGAGCAAACAGAAGCTGATGATCCGAATCGAGCTCATTCAACTCCAAACAGAAACAGCGACCTTGCTACTGCTGCAGTATTGAAAAGGAAAAAAGATGAAACGAAACCCGCGAATAGCAATGTTTCGTTCTGCATTTCAGACGCACTGCCTACTGCATCATCAGCAGATCAAAAGCAAACAGAAGCCGAGGATCTGAATCTAGCTGCAACTGATGCAATATTTGAAAGCAAATCAAAAGAAATGAAATCTGTGGAGAGCTCCCCAGAAGCCGAAAATACTGTTCGAAATGTCACCTCGAATCAAGGAAGAGGCGCTGCATGGAAGCAAAAATCTTCATGGACACAATTAGTTAGCCAGGAAGCCACCTCCTTCAGTATTACTCAAATTTTACCAAACAATACTTCTGAAAAGCAGGTACAAAGGGAATCCGATGTCATCAATGTTAATCTTTTCGCGCCAAGCGAAAATAAGGATTCAATAGAACAGCAAATTCAAAGTACTGCGGCAGATGATTCTGCTGCATTTGTAGTTGCAAAAGATGAAACTGCCTGTTATGATGTCAAGAAGAACGACCAACCTGCAGTGCAAGAAAATGAGCCCTCTCCCACCGAAGCTATTGAGAGGCATATTAAACCACAGGAAGCAGGTTCATTTGATGCTAAAAGCGGCGAAACTTGCTGCCCGTTTATGAGAAATTCTCGGTCGGTAGCCGAGTGGACAAAGATAAAAGCTGCTCTTTCTGGTGGATCCAAGAAGAAAAAGCAGAGACAATTGGACACTCAATAG

Protein sequence

MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSSQSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPESTEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTSKSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTDNQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQSKKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSWKTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKTKPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPANSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNVTSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENKDSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGSFDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ
Homology
BLAST of Carg22712 vs. NCBI nr
Match: KAG7022183.1 (Nucleolar protein 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1345.1 bits (3480), Expect = 0.0e+00
Identity = 704/704 (100.00%), Postives = 704/704 (100.00%), Query Frame = 0

Query: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60
           MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS
Sbjct: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60

Query: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES 120
           QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES
Sbjct: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES 120

Query: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180
           TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS
Sbjct: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180

Query: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240
           KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD
Sbjct: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240

Query: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300
           NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS
Sbjct: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300

Query: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360
           KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW
Sbjct: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360

Query: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420
           KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT
Sbjct: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420

Query: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480
           KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA
Sbjct: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480

Query: 481 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 540
           NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV
Sbjct: 481 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 540

Query: 541 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENK 600
           TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENK
Sbjct: 541 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENK 600

Query: 601 DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 660
           DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS
Sbjct: 601 DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 660

Query: 661 FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 705
           FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ
Sbjct: 661 FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 704

BLAST of Carg22712 vs. NCBI nr
Match: XP_022933918.1 (uncharacterized protein LOC111441186 [Cucurbita moschata])

HSP 1 Score: 1333.2 bits (3449), Expect = 0.0e+00
Identity = 699/704 (99.29%), Postives = 701/704 (99.57%), Query Frame = 0

Query: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60
           MEEGESAS+K+RIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS
Sbjct: 1   MEEGESASSKLRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60

Query: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES 120
           QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLET APES
Sbjct: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETSAPES 120

Query: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180
           TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS
Sbjct: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180

Query: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240
           KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD
Sbjct: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240

Query: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300
           NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS
Sbjct: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300

Query: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360
           KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW
Sbjct: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360

Query: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420
           KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT
Sbjct: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420

Query: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480
           KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA
Sbjct: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480

Query: 481 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 540
           NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV
Sbjct: 481 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 540

Query: 541 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENK 600
           TSNQGRGAAWKQKSSWTQLVSQEATSFSITQIL NNTSEKQVQRESDVINVNLFAPSENK
Sbjct: 541 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILSNNTSEKQVQRESDVINVNLFAPSENK 600

Query: 601 DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 660
           DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS
Sbjct: 601 DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 660

Query: 661 FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 705
           F AKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ
Sbjct: 661 FHAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 704

BLAST of Carg22712 vs. NCBI nr
Match: KAG6588264.1 (Protein REPRESSOR OF SILENCING 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1332.0 bits (3446), Expect = 0.0e+00
Identity = 698/704 (99.15%), Postives = 701/704 (99.57%), Query Frame = 0

Query: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60
           MEEGE AS+KMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS
Sbjct: 49  MEEGEGASSKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 108

Query: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES 120
           QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLET APES
Sbjct: 109 QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETSAPES 168

Query: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180
           TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS
Sbjct: 169 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 228

Query: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240
           KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD
Sbjct: 229 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 288

Query: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300
           NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS
Sbjct: 289 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 348

Query: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360
           KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPD+AMPIQSSRSQKSSW
Sbjct: 349 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDQAMPIQSSRSQKSSW 408

Query: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420
           KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT
Sbjct: 409 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 468

Query: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480
           KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA
Sbjct: 469 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 528

Query: 481 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 540
           NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV
Sbjct: 529 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 588

Query: 541 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENK 600
           TSNQGRGAAWKQKSSWTQLVSQEATSFSITQIL NNTSEKQVQRESDVINVNLFAPSENK
Sbjct: 589 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILSNNTSEKQVQRESDVINVNLFAPSENK 648

Query: 601 DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 660
           +SIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS
Sbjct: 649 NSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 708

Query: 661 FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 705
           FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ
Sbjct: 709 FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 752

BLAST of Carg22712 vs. NCBI nr
Match: XP_023006551.1 (uncharacterized protein LOC111499238 [Cucurbita maxima])

HSP 1 Score: 1282.3 bits (3317), Expect = 0.0e+00
Identity = 671/704 (95.31%), Postives = 684/704 (97.16%), Query Frame = 0

Query: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60
           MEE ESASTKMRIYVGGLGA+MTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS
Sbjct: 1   MEEEESASTKMRIYVGGLGASMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60

Query: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES 120
           QSS+SKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLET APES
Sbjct: 61  QSSISKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETSAPES 120

Query: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180
           TEHVAKSEHIQIFFPSLGEVKS P+SGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPT 
Sbjct: 121 TEHVAKSEHIQIFFPSLGEVKSFPVSGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTG 180

Query: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240
           KSMD KTGDLDAGNGGI EDEIKMMNTVLNKLFERQEASHANCNGTM VKDKDNSKILTD
Sbjct: 181 KSMDTKTGDLDAGNGGIDEDEIKMMNTVLNKLFERQEASHANCNGTMAVKDKDNSKILTD 240

Query: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300
           NQPLEDNKEDSDED+LVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGA RDQKGNSRVQS
Sbjct: 241 NQPLEDNKEDSDEDNLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGAARDQKGNSRVQS 300

Query: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360
           KKRKSVTNEEFD NEYVPNISTGSGKGNTNPAYEPVGPSRPQAPD+AMPIQSSRSQKSSW
Sbjct: 301 KKRKSVTNEEFDGNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDQAMPIQSSRSQKSSW 360

Query: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420
           KTLICDKSKASFSISDILPSVPSANEEQPEAD LSLAHSSPNRNSDRATAAVLKRKKDKT
Sbjct: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADDLSLAHSSPNRNSDRATAAVLKRKKDKT 420

Query: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480
           KPANSNVSFSILDTLPTASS DQEQTEA DPNRAHSTPNRNSDLATAAVLKRKKDETKPA
Sbjct: 421 KPANSNVSFSILDTLPTASSADQEQTEAADPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480

Query: 481 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 540
           NSNVSFCISDALPTASSADQ+QTEAED NLAATDAI ESKSKEMKSVESSPEAENT+ NV
Sbjct: 481 NSNVSFCISDALPTASSADQEQTEAEDPNLAATDAILESKSKEMKSVESSPEAENTIPNV 540

Query: 541 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENK 600
           TSN+GRGAAWK+KSSWTQLVSQEATSFSITQIL NNTSEKQVQRESDVINVNLFAPSEN 
Sbjct: 541 TSNKGRGAAWKKKSSWTQLVSQEATSFSITQILSNNTSEKQVQRESDVINVNLFAPSENN 600

Query: 601 DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 660
           DSIEQ+ +STAAD+SAAFV+AKDETACYDVKKNDQPAVQENEPSPTE IERHIKPQEAGS
Sbjct: 601 DSIEQESRSTAADESAAFVIAKDETACYDVKKNDQPAVQENEPSPTEVIERHIKPQEAGS 660

Query: 661 FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 705
           FDAKS ETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ
Sbjct: 661 FDAKSVETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 704

BLAST of Carg22712 vs. NCBI nr
Match: XP_023520666.1 (papilin [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1256.5 bits (3250), Expect = 0.0e+00
Identity = 674/761 (88.57%), Postives = 684/761 (89.88%), Query Frame = 0

Query: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60
           MEEGESASTKMRIYVGGLGAAMTEDDLRKVF+SVGGVVEAVDFIRSKSRSFAYVDFFPSS
Sbjct: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFKSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60

Query: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES 120
           QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLE  APES
Sbjct: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLEASAPES 120

Query: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180
           TEHVAKSEHIQIFFPSLGEVKSLPISGTG HKYDFPHVEVPPLPVHFCDCEEHNVSDPTS
Sbjct: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGIHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180

Query: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240
           KSMD KTGDLDAGNGG+ EDEIKMMNTVLNKLFERQ+ASH NCN TM VKDKDNSKILTD
Sbjct: 181 KSMDTKTGDLDAGNGGMDEDEIKMMNTVLNKLFERQDASHVNCNETMAVKDKDNSKILTD 240

Query: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300
           NQPLEDNKEDSDED+LVLNVMASGSNSK LPLNSGSKSFKAHGNSKGADRDQKGNSRVQS
Sbjct: 241 NQPLEDNKEDSDEDNLVLNVMASGSNSKSLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300

Query: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360
           KKRKSVT+EEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSS SQKSSW
Sbjct: 301 KKRKSVTDEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSHSQKSSW 360

Query: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420
           KTLICDKSKASFSISDILPSVPSANEEQPEAD LSLAHSSPN+NSDRATAAVLKRKKDKT
Sbjct: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADDLSLAHSSPNKNSDRATAAVLKRKKDKT 420

Query: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSD----------------- 480
           KPANSNVSFSILDTLPTASS DQEQTEADDPNRAHSTPNRNSD                 
Sbjct: 421 KPANSNVSFSILDTLPTASSADQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480

Query: 481 ----------------------------------------LATAAVLKRKKDETKPANSN 540
                                                   LATAAVLKRKKDETKPANSN
Sbjct: 481 NSNVSFSILDTLPTASSADQEQTEAADPNRAHSTPNRNSNLATAAVLKRKKDETKPANSN 540

Query: 541 VSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNVTSN 600
           VSFCISDALPTASSADQ+QTEAEDLNLAATDAI E KSKEMKSVESSPEAENTVRNVTSN
Sbjct: 541 VSFCISDALPTASSADQEQTEAEDLNLAATDAILERKSKEMKSVESSPEAENTVRNVTSN 600

Query: 601 QGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENKDSI 660
           QGRGAAWKQKSSWTQLVSQEATSFSITQIL NNTSEKQVQRESDVINVNLFA SEN DSI
Sbjct: 601 QGRGAAWKQKSSWTQLVSQEATSFSITQILSNNTSEKQVQRESDVINVNLFAASENNDSI 660

Query: 661 EQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGSFDA 705
           EQ+ +STAADDSAAFVVAKDETACYDVKKNDQPAVQENE SPTEAIERHIKPQEAGSFDA
Sbjct: 661 EQESRSTAADDSAAFVVAKDETACYDVKKNDQPAVQENESSPTEAIERHIKPQEAGSFDA 720

BLAST of Carg22712 vs. ExPASy Swiss-Prot
Match: Q9FGT1 (Protein REPRESSOR OF SILENCING 3 OS=Arabidopsis thaliana OX=3702 GN=ROS3 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 7.3e-66
Identity = 247/799 (30.91%), Postives = 372/799 (46.56%), Query Frame = 0

Query: 3   EGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSSQS 62
           E +S+   +R++VGGLG ++  DDL K+F  + G V+AV+F+R+K RSFAY+DF PSS +
Sbjct: 2   EEKSSGGGVRLHVGGLGESVGRDDLLKIFSPM-GTVDAVEFVRTKGRSFAYIDFSPSSTN 61

Query: 63  SVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPESTE 122
           S++KLFSTYNGC WKGG+LRLEKAKEHYLARL+REWE  A     D+     + +P +T 
Sbjct: 62  SLTKLFSTYNGCVWKGGRLRLEKAKEHYLARLKREWE--AASSTSDNTIKAPSDSPPAT- 121

Query: 123 HVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEV-PPLPVHFCDCEEHNVSDPTSK 182
                 H+ IFFP L +VK +P+SGTG HKY F  V V   LP  FCDCEEH+ S  T +
Sbjct: 122 ------HLNIFFPRLRKVKPMPLSGTGKHKYSFQRVPVSSSLPRSFCDCEEHSNSSLTPR 181

Query: 183 SMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTDN 242
            +     DL+A N G  E E+ +MN+V+NKLFE+                          
Sbjct: 182 EIHLH--DLEAVNVGRQEAEVNVMNSVMNKLFEKNNV----------------------- 241

Query: 243 QPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSK-----GADRDQKGNS 302
            P EDN+ ++D+D+L++NV +SG N     L+  S+  K+  N K     G    +KGN 
Sbjct: 242 DPEEDNEIEADQDNLIINVASSG-NDMDSALDMLSRKRKSILNKKTPSEEGYSEGRKGNL 301

Query: 303 RVQSKKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQS---- 362
              SK R++++ EE    E     S+ + +G   P+ E V       P R   ++     
Sbjct: 302 THPSKNRQTISLEETGRQE-----SSQAIRGKKKPS-EVVPDKSSDEPSRTKDLEQSIDN 361

Query: 363 -SRSQKSSWKTLICDKSKASFSISDILPSVPSANEEQP---------------------- 422
            S SQKSSWK+L+ + +   FS+S  LP V S+   QP                      
Sbjct: 362 ISWSQKSSWKSLMANGNSNDFSVSSFLPGVGSSKAVQPAPRNTDLAGLPSRENLKKKTKR 421

Query: 423 ---------------------------------EADALSLAHSSPNRNSDRATAAVLKRK 482
                                            ++DA+    +  +   D A+ +V +R 
Sbjct: 422 KRVTSTIMAEDLPVSDDIKRDDSDTMADDIERDDSDAVEYYTACESMADDTASDSVAERD 481

Query: 483 -----KDKT-------KPANSNVSFS-------------------ILDTLPTASSGDQEQ 542
                +D T        PA+ +V+ S                   + +++  +  GD  +
Sbjct: 482 DSDAVEDDTAIDSMADDPASDSVAESDDGDAVENDTAIDSMADDTVSNSMAESDDGDNVE 541

Query: 543 TEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPANSNVSFCIS---DALPTASSADQKQ 602
            +    +    T N +     +  L     +T      + F  +   D++   S+ ++ +
Sbjct: 542 DDTAIDSMCDDTANDDVGSDDSGSLADTVSDTSVEAVPLEFVANTEGDSVDGKSNVEKHE 601

Query: 603 TEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNVTSNQGRGAAWKQKSSWTQLVSQ 662
             AEDLN      + +      ++V    EA       ++    G++W QK+SWTQLVS 
Sbjct: 602 NVAEDLNAEKESLVVK------ENVVDEEEAGKGPLKASNKSTGGSSWLQKASWTQLVSD 661

Query: 663 EAT-SFSITQILPNNTSEKQVQRESDVINVNLFAPSENKDSIEQQIQSTAADDSAAFVVA 701
           + T SFSITQ+ P+ TS+K     + VIN      S +  +     Q+  A  S  FV A
Sbjct: 662 KNTSSFSITQLFPDLTSDK--GEAAGVINNVGNQFSNSNQTASAMKQTDYASSSGGFVAA 721

BLAST of Carg22712 vs. ExPASy Swiss-Prot
Match: Q3UHX0 (Nucleolar protein 8 OS=Mus musculus OX=10090 GN=Nol8 PE=1 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 1.1e-05
Identity = 33/94 (35.11%), Postives = 52/94 (55.32%), Query Frame = 0

Query: 12  RIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIR-----SKSRSFAYVDFFPSSQSSVSK 71
           R++VGGLG  ++E DL+  F   G V +     R     +  + FAYV+    +++ + K
Sbjct: 9   RLFVGGLGQGISETDLQNQFGRFGEVSDVEIITRKDDQGNSQKVFAYVN-IQITEADLKK 68

Query: 72  LFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEE 101
             S  N   WKGG L+++ AKE +L RL +E E+
Sbjct: 69  CMSILNKTKWKGGTLQIQLAKESFLHRLAQERED 101

BLAST of Carg22712 vs. ExPASy Swiss-Prot
Match: Q76FK4 (Nucleolar protein 8 OS=Homo sapiens OX=9606 GN=NOL8 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.2e-05
Identity = 40/128 (31.25%), Postives = 61/128 (47.66%), Query Frame = 0

Query: 12  RIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSK------SRSFAYVDFFPSSQSSVS 71
           R+YVGGL   ++E DL+  F   G V + V+ I  K       + FAY++    +++ + 
Sbjct: 9   RLYVGGLSQDISEADLQNQFSRFGEVSD-VEIITRKDDQGNPQKVFAYIN-ISVAEADLK 68

Query: 72  KLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEE-----------DAEIMNYDDGADLE 123
           K  S  N   WKGG L+++ AKE +L RL +E E            +A ++    G D  
Sbjct: 69  KCMSVLNKTKWKGGTLQIQLAKESFLHRLAQEREAAKAKKEESTTGNANLLEKTGGVDFH 128

BLAST of Carg22712 vs. ExPASy TrEMBL
Match: A0A6J1F672 (uncharacterized protein LOC111441186 OS=Cucurbita moschata OX=3662 GN=LOC111441186 PE=4 SV=1)

HSP 1 Score: 1333.2 bits (3449), Expect = 0.0e+00
Identity = 699/704 (99.29%), Postives = 701/704 (99.57%), Query Frame = 0

Query: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60
           MEEGESAS+K+RIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS
Sbjct: 1   MEEGESASSKLRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60

Query: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES 120
           QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLET APES
Sbjct: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETSAPES 120

Query: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180
           TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS
Sbjct: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180

Query: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240
           KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD
Sbjct: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240

Query: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300
           NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS
Sbjct: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300

Query: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360
           KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW
Sbjct: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360

Query: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420
           KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT
Sbjct: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420

Query: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480
           KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA
Sbjct: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480

Query: 481 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 540
           NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV
Sbjct: 481 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 540

Query: 541 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENK 600
           TSNQGRGAAWKQKSSWTQLVSQEATSFSITQIL NNTSEKQVQRESDVINVNLFAPSENK
Sbjct: 541 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILSNNTSEKQVQRESDVINVNLFAPSENK 600

Query: 601 DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 660
           DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS
Sbjct: 601 DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 660

Query: 661 FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 705
           F AKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ
Sbjct: 661 FHAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 704

BLAST of Carg22712 vs. ExPASy TrEMBL
Match: A0A6J1KY23 (uncharacterized protein LOC111499238 OS=Cucurbita maxima OX=3661 GN=LOC111499238 PE=4 SV=1)

HSP 1 Score: 1282.3 bits (3317), Expect = 0.0e+00
Identity = 671/704 (95.31%), Postives = 684/704 (97.16%), Query Frame = 0

Query: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60
           MEE ESASTKMRIYVGGLGA+MTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS
Sbjct: 1   MEEEESASTKMRIYVGGLGASMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60

Query: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES 120
           QSS+SKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLET APES
Sbjct: 61  QSSISKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETSAPES 120

Query: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180
           TEHVAKSEHIQIFFPSLGEVKS P+SGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPT 
Sbjct: 121 TEHVAKSEHIQIFFPSLGEVKSFPVSGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTG 180

Query: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240
           KSMD KTGDLDAGNGGI EDEIKMMNTVLNKLFERQEASHANCNGTM VKDKDNSKILTD
Sbjct: 181 KSMDTKTGDLDAGNGGIDEDEIKMMNTVLNKLFERQEASHANCNGTMAVKDKDNSKILTD 240

Query: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300
           NQPLEDNKEDSDED+LVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGA RDQKGNSRVQS
Sbjct: 241 NQPLEDNKEDSDEDNLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGAARDQKGNSRVQS 300

Query: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360
           KKRKSVTNEEFD NEYVPNISTGSGKGNTNPAYEPVGPSRPQAPD+AMPIQSSRSQKSSW
Sbjct: 301 KKRKSVTNEEFDGNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDQAMPIQSSRSQKSSW 360

Query: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420
           KTLICDKSKASFSISDILPSVPSANEEQPEAD LSLAHSSPNRNSDRATAAVLKRKKDKT
Sbjct: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADDLSLAHSSPNRNSDRATAAVLKRKKDKT 420

Query: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480
           KPANSNVSFSILDTLPTASS DQEQTEA DPNRAHSTPNRNSDLATAAVLKRKKDETKPA
Sbjct: 421 KPANSNVSFSILDTLPTASSADQEQTEAADPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480

Query: 481 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 540
           NSNVSFCISDALPTASSADQ+QTEAED NLAATDAI ESKSKEMKSVESSPEAENT+ NV
Sbjct: 481 NSNVSFCISDALPTASSADQEQTEAEDPNLAATDAILESKSKEMKSVESSPEAENTIPNV 540

Query: 541 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENK 600
           TSN+GRGAAWK+KSSWTQLVSQEATSFSITQIL NNTSEKQVQRESDVINVNLFAPSEN 
Sbjct: 541 TSNKGRGAAWKKKSSWTQLVSQEATSFSITQILSNNTSEKQVQRESDVINVNLFAPSENN 600

Query: 601 DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 660
           DSIEQ+ +STAAD+SAAFV+AKDETACYDVKKNDQPAVQENEPSPTE IERHIKPQEAGS
Sbjct: 601 DSIEQESRSTAADESAAFVIAKDETACYDVKKNDQPAVQENEPSPTEVIERHIKPQEAGS 660

Query: 661 FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 705
           FDAKS ETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ
Sbjct: 661 FDAKSVETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQLDTQ 704

BLAST of Carg22712 vs. ExPASy TrEMBL
Match: A0A5D3CSI8 (Nucleolar protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1610G00320 PE=4 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 8.0e-233
Identity = 458/700 (65.43%), Postives = 522/700 (74.57%), Query Frame = 0

Query: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60
           ME G+SAS KMRIYVGGLGAAMTEDDLRKVF SVGGVVEAVDF+R+KSRSFAYVDFFPSS
Sbjct: 1   MERGQSASEKMRIYVGGLGAAMTEDDLRKVFHSVGGVVEAVDFVRTKSRSFAYVDFFPSS 60

Query: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES 120
           QSS+SKLFSTYNGCAWKGGKLRLEKAKE+YLARL+REWEEDA+I + + GAD+E  APES
Sbjct: 61  QSSLSKLFSTYNGCAWKGGKLRLEKAKENYLARLKREWEEDAQIRDSNVGADMEVVAPES 120

Query: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180
           T+HV KSEHI IFFPSLGEVKSLPISGTGTHKYDFPHVEVPP PVHFCDCEEH+VS P  
Sbjct: 121 TQHVTKSEHINIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPFPVHFCDCEEHDVSSPIG 180

Query: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240
            S D KT DL+A NGG+ EDEI+MMN V+NKLFER+EAS +NCNG+M + DK NS +LTD
Sbjct: 181 NSKDTKTRDLNAENGGMAEDEIEMMNAVMNKLFEREEASQSNCNGSMALNDKHNSTMLTD 240

Query: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300
           NQ LEDNK D DED+LVLNVMAS  NSK + LNSG+K FKAHGNSK A RDQK N RVQ 
Sbjct: 241 NQLLEDNKVDCDEDNLVLNVMASNCNSKSMALNSGNKIFKAHGNSKDAVRDQKNNCRVQG 300

Query: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360
           KKRKS  +EEFD NE VP+I T +  G T+P+Y+P   SRPQAPDR  P+QS RSQKS W
Sbjct: 301 KKRKSFLSEEFDGNESVPSIFTSN--GGTDPSYDPARSSRPQAPDRGPPVQSLRSQKSLW 360

Query: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420
           KTLI DKS  SF ISDIL SVPSANEE+ EAD LS+AHS+PN+NSD A AAVL  K D+ 
Sbjct: 361 KTLIRDKSNVSFCISDILCSVPSANEEKSEADDLSIAHSTPNKNSDLARAAVLGSKTDEI 420

Query: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480
           +    NVSF+I + LP+  S DQE+  + D N AHSTPN N+D+                
Sbjct: 421 QSGKINVSFNITEVLPSVPSADQEEAASADLNLAHSTPNINTDV---------------- 480

Query: 481 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 540
                                            D I +SKS+EMKSVES  +A+ TV NV
Sbjct: 481 -------------------------------GADPISKSKSEEMKSVESFLDAQCTVPNV 540

Query: 541 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENK 600
            SN+GRG++W+QKSSWTQLVS+E TSFSITQILPNNTS KQVQ E+   N N    SE  
Sbjct: 541 NSNKGRGSSWRQKSSWTQLVSEEITSFSITQILPNNTSGKQVQGEAGASNANFSLWSETN 600

Query: 601 DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 660
              +Q  +  A D+S AFV+ KDE    DVKKN+  AVQE E  PT+ IE ++ PQ+ GS
Sbjct: 601 APKKQDSECIAEDESTAFVIGKDEIDSNDVKKNEPQAVQECETCPTQIIESNL-PQQGGS 649

Query: 661 FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQ 701
           FD  SGET CPFMRNS+SVAEWTKIKAALSGGSKKKKQRQ
Sbjct: 661 FDVISGET-CPFMRNSQSVAEWTKIKAALSGGSKKKKQRQ 649

BLAST of Carg22712 vs. ExPASy TrEMBL
Match: A0A1S3B9A4 (uncharacterized protein LOC103487200 OS=Cucumis melo OX=3656 GN=LOC103487200 PE=4 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 8.0e-233
Identity = 458/700 (65.43%), Postives = 522/700 (74.57%), Query Frame = 0

Query: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60
           ME G+SAS KMRIYVGGLGAAMTEDDLRKVF SVGGVVEAVDF+R+KSRSFAYVDFFPSS
Sbjct: 1   MERGQSASEKMRIYVGGLGAAMTEDDLRKVFHSVGGVVEAVDFVRTKSRSFAYVDFFPSS 60

Query: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES 120
           QSS+SKLFSTYNGCAWKGGKLRLEKAKE+YLARL+REWEEDA+I + + GAD+E  APES
Sbjct: 61  QSSLSKLFSTYNGCAWKGGKLRLEKAKENYLARLKREWEEDAQIRDSNVGADMEVVAPES 120

Query: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180
           T+HV KSEHI IFFPSLGEVKSLPISGTGTHKYDFPHVEVPP PVHFCDCEEH+VS P  
Sbjct: 121 TQHVTKSEHINIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPFPVHFCDCEEHDVSSPIG 180

Query: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240
            S D KT DL+A NGG+ EDEI+MMN V+NKLFER+EAS +NCNG+M + DK NS +LTD
Sbjct: 181 NSKDTKTRDLNAENGGMAEDEIEMMNAVMNKLFEREEASQSNCNGSMALNDKHNSTMLTD 240

Query: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300
           NQ LEDNK D DED+LVLNVMAS  NSK + LNSG+K FKAHGNSK A RDQK N RVQ 
Sbjct: 241 NQLLEDNKVDCDEDNLVLNVMASNCNSKSMALNSGNKIFKAHGNSKDAVRDQKNNCRVQG 300

Query: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360
           KKRKS  +EEFD NE VP+I T +  G T+P+Y+P   SRPQAPDR  P+QS RSQKS W
Sbjct: 301 KKRKSFLSEEFDGNESVPSIFTSN--GGTDPSYDPARSSRPQAPDRGPPVQSLRSQKSLW 360

Query: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420
           KTLI DKS  SF ISDIL SVPSANEE+ EAD LS+AHS+PN+NSD A AAVL  K D+ 
Sbjct: 361 KTLIRDKSNVSFCISDILCSVPSANEEKSEADDLSIAHSTPNKNSDLARAAVLGSKTDEI 420

Query: 421 KPANSNVSFSILDTLPTASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPA 480
           +    NVSF+I + LP+  S DQE+  + D N AHSTPN N+D+                
Sbjct: 421 QSGKINVSFNITEVLPSVPSADQEEAASADLNLAHSTPNINTDV---------------- 480

Query: 481 NSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNV 540
                                            D I +SKS+EMKSVES  +A+ TV NV
Sbjct: 481 -------------------------------GADPISKSKSEEMKSVESFLDAQCTVPNV 540

Query: 541 TSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVNLFAPSENK 600
            SN+GRG++W+QKSSWTQLVS+E TSFSITQILPNNTS KQVQ E+   N N    SE  
Sbjct: 541 NSNKGRGSSWRQKSSWTQLVSEEITSFSITQILPNNTSGKQVQGEAGASNANFSLWSETN 600

Query: 601 DSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERHIKPQEAGS 660
              +Q  +  A D+S AFV+ KDE    DVKKN+  AVQE E  PT+ IE ++ PQ+ GS
Sbjct: 601 APKKQDSECIAEDESTAFVIGKDEIDSNDVKKNEPQAVQECETCPTQIIESNL-PQQGGS 649

Query: 661 FDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQ 701
           FD  SGET CPFMRNS+SVAEWTKIKAALSGGSKKKKQRQ
Sbjct: 661 FDVISGET-CPFMRNSQSVAEWTKIKAALSGGSKKKKQRQ 649

BLAST of Carg22712 vs. ExPASy TrEMBL
Match: A0A0A0LXQ1 (RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G651690 PE=4 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 2.1e-225
Identity = 454/708 (64.12%), Postives = 518/708 (73.16%), Query Frame = 0

Query: 1   MEEGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSS 60
           ME+G+SAS  MRIYVGGLGAAMTEDDLRKVF SVGGVVEAVDF+R+KSRSFAYVDFFPSS
Sbjct: 1   MEKGQSASENMRIYVGGLGAAMTEDDLRKVFHSVGGVVEAVDFVRTKSRSFAYVDFFPSS 60

Query: 61  QSSVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPES 120
           QSS+SKLFSTYNGCAWKGGKLRLEKAKE+YLARL REWEEDA+I + + GAD+E  APES
Sbjct: 61  QSSLSKLFSTYNGCAWKGGKLRLEKAKENYLARLNREWEEDAQIRDNNVGADMELVAPES 120

Query: 121 TEHVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEVPPLPVHFCDCEEHNVSDPTS 180
           TEHV KSEHI IFFPSLGEVK LPISGTGTHKYDFPHVEVPP PVHFCDCEEHN S P  
Sbjct: 121 TEHVTKSEHINIFFPSLGEVKPLPISGTGTHKYDFPHVEVPPFPVHFCDCEEHNASSPIG 180

Query: 181 KSMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTD 240
            S   KT DL+A NGG+ EDEIKMMN VL+KLFER+EAS +NCN +M + DK NS   TD
Sbjct: 181 NSKYTKTRDLNAENGGMDEDEIKMMNAVLSKLFERKEASQSNCNDSMALNDKHNSTTSTD 240

Query: 241 NQPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSKGADRDQKGNSRVQS 300
           NQ LEDNK DSDED+LVLNVMAS  NSK + LN G+K FKAHGNSK A RDQK N RVQS
Sbjct: 241 NQLLEDNKVDSDEDNLVLNVMASNCNSKTMALNRGNKIFKAHGNSKDAVRDQKNNCRVQS 300

Query: 301 KKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQSSRSQKSSW 360
           KKRKS  +EEFD NE VP+I T S +G T+P+Y+P   SRPQAPDR  P+QS RSQKSSW
Sbjct: 301 KKRKSFISEEFDGNESVPSIFT-SNRG-TDPSYDPARSSRPQAPDRGPPVQSLRSQKSSW 360

Query: 361 KTLICDKSKASFSISDILPSVPSANEEQPEADALSLAHSSPNRNSDRATAAVLKRKKDKT 420
           KTLI DKS  SF ISDIL SVPSANEE+ EAD L++AHS+PNRNS+ A+ AVL  + D+ 
Sbjct: 361 KTLIRDKSNVSFCISDILSSVPSANEEKAEADDLNIAHSTPNRNSNLASTAVLGSEIDEI 420

Query: 421 KPANSNVSFSILDTLP--------TASSGDQEQTEADDPNRAHSTPNRNSDLATAAVLKR 480
           +    NV FSI D LP         A+S DQE+  + D N AHSTPN N+D+        
Sbjct: 421 QSGKINVPFSITDVLPLVLSADQEKAASADQEKAASADLNLAHSTPNINTDV-------- 480

Query: 481 KKDETKPANSNVSFCISDALPTASSADQKQTEAEDLNLAATDAIFESKSKEMKSVESSPE 540
                                                    D I +SKS+EM+SVES  +
Sbjct: 481 ---------------------------------------GADPISKSKSEEMESVESFQD 540

Query: 541 AENTVRNVTSNQGRGAAWKQKSSWTQLVSQEATSFSITQILPNNTSEKQVQRESDVINVN 600
           A+ TV NVT N+GRG++W++KSSWTQLVS+E TSFSITQILPN+TSE QVQ ES  IN N
Sbjct: 541 AQCTVPNVTLNKGRGSSWRKKSSWTQLVSEEFTSFSITQILPNSTSENQVQGESGDINAN 600

Query: 601 LFAPSENKDSIEQQIQSTAADDSAAFVVAKDETACYDVKKNDQPAVQENEPSPTEAIERH 660
             A SE     +Q  +  A D+S AFV+ K E  C DVK+N+  AVQE E  PT+  E +
Sbjct: 601 FSAWSETNAPRKQDSECIAKDESTAFVIGKGEIGCNDVKQNEPQAVQECETCPTQITESN 657

Query: 661 IKPQEAGSFDAKSGETCCPFMRNSRSVAEWTKIKAALSGGSKKKKQRQ 701
             PQ+ GSFD  SG+T CPFMRNS+SVAEWTKIKAALSGGSKKKKQRQ
Sbjct: 661 F-PQQEGSFDEISGDT-CPFMRNSQSVAEWTKIKAALSGGSKKKKQRQ 657

BLAST of Carg22712 vs. TAIR 10
Match: AT5G58130.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 253.4 bits (646), Expect = 5.2e-67
Identity = 247/799 (30.91%), Postives = 372/799 (46.56%), Query Frame = 0

Query: 3   EGESASTKMRIYVGGLGAAMTEDDLRKVFQSVGGVVEAVDFIRSKSRSFAYVDFFPSSQS 62
           E +S+   +R++VGGLG ++  DDL K+F  + G V+AV+F+R+K RSFAY+DF PSS +
Sbjct: 2   EEKSSGGGVRLHVGGLGESVGRDDLLKIFSPM-GTVDAVEFVRTKGRSFAYIDFSPSSTN 61

Query: 63  SVSKLFSTYNGCAWKGGKLRLEKAKEHYLARLRREWEEDAEIMNYDDGADLETYAPESTE 122
           S++KLFSTYNGC WKGG+LRLEKAKEHYLARL+REWE  A     D+     + +P +T 
Sbjct: 62  SLTKLFSTYNGCVWKGGRLRLEKAKEHYLARLKREWE--AASSTSDNTIKAPSDSPPAT- 121

Query: 123 HVAKSEHIQIFFPSLGEVKSLPISGTGTHKYDFPHVEV-PPLPVHFCDCEEHNVSDPTSK 182
                 H+ IFFP L +VK +P+SGTG HKY F  V V   LP  FCDCEEH+ S  T +
Sbjct: 122 ------HLNIFFPRLRKVKPMPLSGTGKHKYSFQRVPVSSSLPRSFCDCEEHSNSSLTPR 181

Query: 183 SMDAKTGDLDAGNGGIGEDEIKMMNTVLNKLFERQEASHANCNGTMGVKDKDNSKILTDN 242
            +     DL+A N G  E E+ +MN+V+NKLFE+                          
Sbjct: 182 EIHLH--DLEAVNVGRQEAEVNVMNSVMNKLFEKNNV----------------------- 241

Query: 243 QPLEDNKEDSDEDSLVLNVMASGSNSKPLPLNSGSKSFKAHGNSK-----GADRDQKGNS 302
            P EDN+ ++D+D+L++NV +SG N     L+  S+  K+  N K     G    +KGN 
Sbjct: 242 DPEEDNEIEADQDNLIINVASSG-NDMDSALDMLSRKRKSILNKKTPSEEGYSEGRKGNL 301

Query: 303 RVQSKKRKSVTNEEFDSNEYVPNISTGSGKGNTNPAYEPVGPSRPQAPDRAMPIQS---- 362
              SK R++++ EE    E     S+ + +G   P+ E V       P R   ++     
Sbjct: 302 THPSKNRQTISLEETGRQE-----SSQAIRGKKKPS-EVVPDKSSDEPSRTKDLEQSIDN 361

Query: 363 -SRSQKSSWKTLICDKSKASFSISDILPSVPSANEEQP---------------------- 422
            S SQKSSWK+L+ + +   FS+S  LP V S+   QP                      
Sbjct: 362 ISWSQKSSWKSLMANGNSNDFSVSSFLPGVGSSKAVQPAPRNTDLAGLPSRENLKKKTKR 421

Query: 423 ---------------------------------EADALSLAHSSPNRNSDRATAAVLKRK 482
                                            ++DA+    +  +   D A+ +V +R 
Sbjct: 422 KRVTSTIMAEDLPVSDDIKRDDSDTMADDIERDDSDAVEYYTACESMADDTASDSVAERD 481

Query: 483 -----KDKT-------KPANSNVSFS-------------------ILDTLPTASSGDQEQ 542
                +D T        PA+ +V+ S                   + +++  +  GD  +
Sbjct: 482 DSDAVEDDTAIDSMADDPASDSVAESDDGDAVENDTAIDSMADDTVSNSMAESDDGDNVE 541

Query: 543 TEADDPNRAHSTPNRNSDLATAAVLKRKKDETKPANSNVSFCIS---DALPTASSADQKQ 602
            +    +    T N +     +  L     +T      + F  +   D++   S+ ++ +
Sbjct: 542 DDTAIDSMCDDTANDDVGSDDSGSLADTVSDTSVEAVPLEFVANTEGDSVDGKSNVEKHE 601

Query: 603 TEAEDLNLAATDAIFESKSKEMKSVESSPEAENTVRNVTSNQGRGAAWKQKSSWTQLVSQ 662
             AEDLN      + +      ++V    EA       ++    G++W QK+SWTQLVS 
Sbjct: 602 NVAEDLNAEKESLVVK------ENVVDEEEAGKGPLKASNKSTGGSSWLQKASWTQLVSD 661

Query: 663 EAT-SFSITQILPNNTSEKQVQRESDVINVNLFAPSENKDSIEQQIQSTAADDSAAFVVA 701
           + T SFSITQ+ P+ TS+K     + VIN      S +  +     Q+  A  S  FV A
Sbjct: 662 KNTSSFSITQLFPDLTSDK--GEAAGVINNVGNQFSNSNQTASAMKQTDYASSSGGFVAA 721

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022183.10.0e+00100.00Nucleolar protein 8 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022933918.10.0e+0099.29uncharacterized protein LOC111441186 [Cucurbita moschata][more]
KAG6588264.10.0e+0099.15Protein REPRESSOR OF SILENCING 3, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023006551.10.0e+0095.31uncharacterized protein LOC111499238 [Cucurbita maxima][more]
XP_023520666.10.0e+0088.57papilin [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FGT17.3e-6630.91Protein REPRESSOR OF SILENCING 3 OS=Arabidopsis thaliana OX=3702 GN=ROS3 PE=2 SV... [more]
Q3UHX01.1e-0535.11Nucleolar protein 8 OS=Mus musculus OX=10090 GN=Nol8 PE=1 SV=2[more]
Q76FK44.2e-0531.25Nucleolar protein 8 OS=Homo sapiens OX=9606 GN=NOL8 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F6720.0e+0099.29uncharacterized protein LOC111441186 OS=Cucurbita moschata OX=3662 GN=LOC1114411... [more]
A0A6J1KY230.0e+0095.31uncharacterized protein LOC111499238 OS=Cucurbita maxima OX=3661 GN=LOC111499238... [more]
A0A5D3CSI88.0e-23365.43Nucleolar protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1610... [more]
A0A1S3B9A48.0e-23365.43uncharacterized protein LOC103487200 OS=Cucumis melo OX=3656 GN=LOC103487200 PE=... [more]
A0A0A0LXQ12.1e-22564.12RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G651690 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT5G58130.15.2e-6730.91RNA-binding (RRM/RBD/RNP motifs) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 12..84
e-value: 4.1E-7
score: 39.6
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 13..57
e-value: 6.3E-7
score: 29.1
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 11..88
score: 12.628556
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 2..91
e-value: 1.4E-12
score: 49.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 522..549
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 258..281
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..329
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 346..361
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 283..310
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 380..481
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 223..361
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 423..445
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 530..549
NoneNo IPR availablePANTHERPTHR23099TRANSCRIPTIONAL REGULATORcoord: 1..698
IPR034138Nucleolar protein 8, RNA recognition motifCDDcd12226RRM_NOL8coord: 12..87
e-value: 2.7731E-21
score: 86.4575
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 9..89

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22712-RACarg22712-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005730 nucleolus
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding