Carg22477 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22477
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionserine/threonine-protein kinase Nek6-like
LocationCarg_Chr03: 1065780 .. 1074258 (+)
RNA-Seq ExpressionCarg22477
SyntenyCarg22477
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTCTTTCAAATGGGTTTGTCGACATCTCATCTGCCTGCCCTCAATTTGAAGCTCTTATGAATCTGCTAATCGAAGCAACGCTTTTGGGTTTGCCTTTCCCTTTCATCTTCCTAACTTCCCACTTTCCCTTTCTCTTTTGAGTTTGTCATTCCCTTCACATTCTTTACAACATCATCATAATTTCACGGTTGCTTTACTCATTGATTGTGTCTCTGTAGATTCTGGAAATTCCAGTTATAATTGAAAGACTTATAGCTTTTGGGGGAAGTTTTTGGTTCAAACTTACCCAATTTGGTGTTGGATTTTGATTTTTGTTAAGAAGTTTTCGCCATGTGAGAGTTTTGCTAACTGTTTTCGATTTCGAAATGGTTTTTGTTTGGGGGTTTTGGCCTTAACACTGTTTTTTTGGCGTTTGAAGGACGACATGGAGTGTGATGATGATGATATGAAGAACAGGATGGAAGATTATGAAGTTCTTGAGCAGATTGGGAGGGGAGCTTTTGGATCAGCTTTTCTTGTTTACCATAAGATTGAGAAGAAAAAGTAAGTAATATACCCTGATGTTCATACTTCCATGTAATAATTGAGAGATCATGGTTAGTTTCGTTCTCTGTTGTTAACGGTGTTGAGGATTTTCGGGAGAGGAGTCCCACGTCGGTTAATTAAGTGTAAGGTCTTTTGGGGAAACTTAAAGCAAAGCCACGAGAGCTTATGCTCGAAGTGGACAATATCATACCATTGTGGAGGTTTGTGATTCCTAACAAATGGAACCACCCTTATCTTCAGAACTAAACTCGGTCTCGAATCTTTTCTTTCTGTTCTTTCACTGTAAATGAAGAAAGACTCGAGAAAAGGGTAATTCGAGATCGATGCCTTGTTTTCTATCGTTGAAATGAAGCAAATGGTTCGGTGTTGTGGCACCACGAAACGTTCGGTGTATGTAGTGCTTCTCTTAATATTTACCTTCTTTCTGAAATAGGTATGTACTCAAGAAAATTCGCTTGGCTAAACAAACGGAGAAGTTCAAGCGCACTGCACACCAGGAGGTTAGTTTATTGGAAACTTATATGAGAAATGTTTGAATTATGAGTCATTGTTTGTGTGAGATCCCACATCGGTTTCGGTTGGGGAGGAGAACAAAGCATTCTTTATAAGAGCGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAAATCTTGAGGGGAAGCCCCGAAGGGAAAGCCCAAAGAGGGCAATATTTGCTAGCGGTGGGCGTTACAGCCTGCATCATATTCAACAGCAATGCTTAACGATCCAAAATATATCACATAGAAAGTTGAGAAAGAAGAAAGTTTATGTCTACAAATGTGGGCATAGGGAACAAGATTAGGATATGTTGTGTTGGTAACTAAGAGAAGAGCGGACATGGGGACTTGGGTTAGGGCGTAGCATAACTACGAGAACCATTCCATTTAGGGCGAGCCCGTGTGGTTATGGTGGCTGACACGAGCGGTCTAGTGTCTGAGCCAATTGGTCAGACGACGATGACTGGACTTATTAGATTAGTATTAGCCGCCTATCCCGAAAGAAAATGGGATAATAGAAAGAACGGGAAGCGCAGGAGCTATAGGATTGGTTTTCCCCGATCGACAGTTGCTGGGTTTCTCCATCTCTCCTAAACTTCCCCCGACATGGCAAACTCTCTCACTTATCTCGAGTTGTAACTAATAAGATATCTTGGGTTGGTACAATGGTTAAATGGGCAAGTTGAACACCTAGATACTAAGCTCATTTTTCAAACTATAGTGTTTACATAGAGGATGTTGAACATCTTAAGGAGCTTCTTGATATTATGTCTTGTTGGATTGGGGGGTTGTCGTGAATGACAAGTCGAGTGTGCTTGGTTGGCTCGAAATCGAAAGAAATGGATACCTCATTTTCATCCTCGGCATGAACTTGACGTATTTTCTTGTCGGGATTAACATAATTAGTTGATGTGTAAGTATATATATATTTATTCATGTGACCTTCTCAATTTCAGATGAATTTGATTGCAACACTAAATCACCCATATATTGTGGACTACAAAGATTCATGGGTGGATAAGGTACATTTTCTTTCTTTTGCCTACTATTCAAATCTGGATTGAATTTGTTTCTTTTACATTAAGAACTCTTTCTTTTGTTTTCTTAGTTCTTATGTAGATTTTCTTTTAGCCTACCTTTTACGTATAATATGTAGATTTTCTTTCTGGCCCCGAGGGGTGGATTGTGAGATCCCACGTCGGTTGGATATTCCTTATAAGGGTATGAAAATCTCTTCCTAGTAGACGCGTTTTATAAAGCTTAAGAGAAGCCCAAAACGGAAAGCCAAAAAGGACAATATCTGCTAGCAATGGACTTTGGCTATTACAAATGATATCAGAGCCAGATACCGGGCAGTGTGCCAGCGAGGACGTTGGGCTCCCAAGGGGGTGGATTGTGAGATCCCACATCGGTTGGACATTCCTTGTAAGAGTATGAAAACCTTTCTTAGTAGACGCGTTTTAAAAAACCTTGAGGAGAAGCCCAAAACGGAAAGCCCAAAAAGGACAATATCTGCTAGCAATGGACTTTGGCTATTACAAATGATATCAGAGCTAGATACCGGGCGGTGTGACAGCGAGGACATTGGGCTCCCAAGGGGGTGGATTGTGAGATCCCACGTTGGTTGGACATTACTTATAAGGGTATGGAAACATTTTTTTAGTAGACTTGTTTTAAAAACCTTGAGGAGAAGCCCAAAAAGGACATTATCTGCGTAGACTTTGGCTATTACAAATGGTATCAAAGCCAAATACCGGGCGGTGTGCCAACCAGGACGTTGGGCCATCAAGGGGGGTGGATTGCGAGATCCCACATTGGCTGAACATTCCTTATAAGTGTGTGGAAACCTCTTCCTAGTAGACACGTTTTAAAACCTTGAGGAAAGCTCGAAAGAGAAAGCCCAAAGAAGACAATATCTGCTAACAGTGGACTTTGGCTATTACAAATGGCATCAGCTAGACACCCGACGGTGTGCCAACAAGGACGTTGGGCCCCCAAGGAGGGTGGATTGTGAGATCACACATCGGTTGAACATTCCTTTCTATTAGACGTGTTTTAAAAACCTTGAGGGGAAGCCCGAAAGGGCCAAAAAAGGATAATATCTACTAGCGATGGGCTTGAGCCATTACAATTAATCTATTGTTTATAGAATATACTTATAGCTCGGTGATTTCTCTGCCTTCTCGTTTTCACTTGCTCTACATTTCATATCTCGGGCGTTCTGTTTTGTAGGGTGATTGTATATGCATCGTAACGGGCTATTGCGAAGGAGGAGACATGTAAGTGAGCTATTTAATTTTACACCATTTGGAGTATGCTTCATTACGACCTTTAAAGCATTAGCTTAATTGTTTGTTGTTGACTTAATTCGAGAACAAAAGTTGTTCAATTTATTTTTCCAACTACATGAACCTTCTCATGTCTTCCACTATCATCCACTGTTGCATATGAACAGGATAAAAATTTGGTTAAAAAAATCCTTCCCATTGGTACAAATTCAAAAGAAAACGGCTACAAAATTCTCCCTCTCACCGTCTCTCGTTTTACTCGTTCCCTTCCTGTTGTGAGATCCCACATCAGTTGGAGAGGAGAACAAAGCATTCGTTATAAGAGTGTGGAGACCTCTCCATAGTAGATGTGTTTTAAAATTGTGAGGCTGACGGCAATACGTAACGGGCCAAAACGGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACAAATGGTATCAAAGCCAGACACTGGGCGGTGTGTAAGCGAGGACGCTGGCCTCCAAGGGGGGTGGATTGTGAGATCCCACATCGATTGGAGAGGAAAACGAAACATTCCATATAAGAGTGTGGAGACTTCTCCCTAGTAGACGCGTTTTAAAATCGTGAGACTGACGGCGATACGTAACGGGCCAAAGCGGACAATATTTGCTAGCGGTGGGCTTGGATTGTTACAAATGGTATCAAAGCCAGACACCGGGCGGTGTGCCAGCGAGGACGCTGCCTCCAAGGGGGGTGGATTGTGAGATCCCACATCGTTTGGAGTGGAAAATGAAACATTCCATATAAGAGTGTGGAGACTTCTCCCTAGTAAATGCGTTTTAAAATCGTGAGACTGACGGCAATACGTAACAGGCCAAAGCGGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACAAATGATATCAAAGCCAGCCACCGGGCAGTGTGCCAACGAGGACGTTGCCTCCAAGGGGGGTGGATTGTGAGATCCCACATCGTTTGGAGAGGAAAACGAAACATTCCATATAAGAGTGTGGAGACTTCTCCCCAGTAGACGCGTTTTAAAATCATGAGATTGATGGCGATACGTAACAGGCCAAAGTGGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACAAATGGTATCAAAGCCAGCCACCGGGCAGTGTGCCAGCGAGGACGTTGCCTCCAAGGGGGGTGGATTGTGAGATCCCACATCGTTTGGAGAGAAAAACGAAACATTCCATATAAGAGTGTGGAGACTTCTCCCCAGTAGACACGTTTTAAAATCATGAGATTGATGGCGATACGTAACAGGCCAAAGTGGACAATATCTGCTAGCGGTGGGCTTGGGTTGTTACAAATGGTATCAAAGCCAGACACCGGGTGGTGTGCCTGCGAGGACGCTGGCCTCCAAAGGGGGTGGATTGCGAGATCCCACTTTGGTTGGAGAGGAAAACGAAACATTCCATATAAGAGTGTGGAGACTTCTCCCTAGTAGACGCACTTTGGTTGGAGAGGAAAACGAAACATTCCATATAAGAGTGTGGAGACTTCTCCCTAGTAGACGCGTTTTACAACAGTGAGGCTGACGGCGATACGTAACAGGCCAAAGCAAACAACGAAGTAACAGCCCAAGCCCACCACCACTAACAGATATTGTCCAATTTGGGTTTTCCCGTTCAGACTTCCCCTCAAGATTTTTAAAACGCGTCTCTAGGGAGCGGTTTCCACACTCTTATAAAGAATGCTTCGTTCTCTTCCCCAACCGATGTGGGATCTGACAATCCACCCCCTTTGGGGCCCAACGTCCTCGCTGACACTCGTTCCTCTCTCCAATCGATGTGGGATCTCACACTTTCCATTGTCGAACATCTTATTCTCATGTTGTTGACAACATGACCATTTGACCGAAGATTAGGTATCCTCCAAAGAGATTGGTGAGGGATGGTCACTCATCAAGAACCCTTCCATATGCCATAAATAACAAAAACAAAAACAAAAATTCTTTGATGACAAAAAATCAACATTAATCATATTACTTAACATTGAATGGATAAAGGAATGAGAAGAAAACTCTAAGACTAATGGTATGCCCCATTTTCTTTTGTTGAGCAGGTCCGAACTGATCAAAAAGGCCAAAGGAACATACTTTCCCGAGGAAGTAAGTCGAGCACGTTCTCAACTATTCTTTCTATACCGAATCTATGCCCGATGACCAATTCTTTTTTCCTCAGAAACTTTGCAAGTGGTTGGCGCAGTTGTTACTAGCTGTGGACTACCTCCACTCGAATCGAGTGCTACATAGAGATCTTAAGGTATTTATTCCGTATTATTAACCTACAAAAATCTCATTAATGATTCTGATCTTTTGTTCTTCATTTCAGTGTTCCAACATTTTCCTTACAAAGGGCAATGATATCAGGCTTGGTATGATGGCTTCCAATTTGCTAGCTATTTGTTCTTGAAAAGTGCTAATATCTTGAATGGGTGGCATGTGTAGGGGACTTCGGACTTGCAAAACTACTGAATACGGAAGATCTTGCTTCATCGGTAAGGATGCGATCATCGCCTGTAGTTATTTTGCGATAGAAAGCGTTTTAATCAACAAACGTATCTTTTATGCTGTTAAAATCGGGTCTGACAAAGGACTTAGAATGTTTAACTTACGGAGTTGTACGTTGGCTAATTAAGAGGATGATCATGGTTGAGAGCTTATGCTCGAAGTGGATAAATATCATACCTTTGTGAAGGATCGTGATTACTAACATGGTATCGGAGTCATGCCCTTAACTTAGCGATGTCAATCAAATCCTCAAGTGTCGAACAAATAAGTTGTTGGGTCTCAAAGGTATAGTTAAAGGTGAGTAAGTGTTGAACAAATGGTGTAATTTGTTCGAGGGCTCTCGAGGAATCGAGCCTCGATTAAGGGAAGGTTGTTTGAGGGTTTTATAGGTCTCAGGGGAGGCTCTATAGTGTACTTTGTTCGAGGGGAGGATTGTTGAGGGGGAGTCCCACATCGACTATTTAAGAGGATGATCATGAGTTTATAAGTAAGGAATACATCAAAAGTAAAGCCACGAGAGCTTAGGATCAATGTGGAAAATGTCATCATTGTGGAGGGTCGTGGTTTCACAACCCAAGCCCACCACTGACCGATATTGTTCGCTTTGGACTTGAAGGGAACGTGTCTCCTAGGGGAGGAGCAAGCGAATGGAGGAAACTATTATTAATGTAACGGCCCAAGCACATGTTGTTCTCTTTGGGCTTTCCCTTGAGGGGAATGCGTCAGCTAGGGGAGGTTTTCACACCCTTATAAAAGATACTTCGTTCTCCTTCTAAATCGATGTGGGATGTCACAATCCCACCCCCCTTTGGGGTCAGCGTCCTCGCTGGCACTCGTTTCCGTCTCCAATCGACGTGGGATCTTACAATCCACCCCCTACCATTTATAAAAGCTCAAGCCTACCGCTAGCAAATAATATTGTCGTTGACACTCGCTCTCCAATCAACGTGGGATCTCACAATCCACCCCCTCCCATTTGTAAAAGCCAAAGCCCGCTAGCAAATATTGTCCTCTACTAGGAAGAATTTCCCGCTTATAAAGAATGTTTCATTCAAGATCATAGAGATAAAGTAATTACGTAATAGTTCCGGAATAATAATTTGATTGTACTCGACGCTAATATATGTTCTGTAGGTTGTTGGTACTCCTAATTACATGTGCCCCGAGCTCCTCACAGATATACCTTATGGCTACAAGTCCGATATATGGTCACTTGGTAAGCGTTCGTGGCAACACGATTCTTGGTTGATATCCGTGTTCTTGGATAGTAATAAACCGTGTTTTTTCCAGGTTGCTGTATGTTTGAAATTTCTGCTCATCAACCTGCTTTTCGAGCTCCGGTAAGCTTAAGCTTCGGTCTCGTGAATTTCATCCGTTTTGCTCGACTCCTGATTGATTTATGCTTAATTCAACAGGACATGGCTGGTCTTATCAATAAAATTAACAGATCCTCCATTTCTCCTCTCCCAATTGTTTATTCCTCTGCGTTGTAAGCTATTTCTATTGATTTTTCAGCTAAAAACAGTGTATTTTCCGCTATTTTAAGACTTCGAGACTGTTCGTTTCGACTTGCAGGAAACAGATCATCAAGAGCATGCTACGGAAGAATCCCGGACACAGACCGACGGTGAGTGATGCGATTTCGACTCTTTGAATGATCATTTTGCTTCTAATCCATGGCTTAATTTATATTTAGGCTGCTGAATTATTGAGGCATCCACATATCCAACCGTATGTCCTTCAATGCCGCAACACTTCGGCTAATATACTACCGATATGTCCCGACTCAAAGAATAAAACACCACGAAAATCGCCATCTAACAAGTTGAATAGTGGGCATGAGAGCCGAGGCAAACCGAGCGTAGTTTTCAACCTGCTGGAAAATATCGATCTGGTGGCCGGAAGTGCTGACATACATAATAGCAACTCGTCAACTGATGAAAATATGTCGTTAACAGCTATTTCGGAAGACATACTCGAGACGAAGATGGTCGATCCTACATCCTTTCCTGATGAAATATCCACATCAAATGCTGTTGATACTGATGCGCAAACGAGTTCTGCGTCTGTGACATCGACTTGCAATGGTGGAAACAGCATCCGGTGTACGAATTCTTTGCAGAAAGAACCTCGATTGGAGGACGTACCGAAAACAAGTTGCAACGAACCGGGAACGAAGGCTGAGGAAACTAGTGAAGGGAACCCAATGGAATCATCGATATCCTCCATACGCAGCACTGAAAAGTCCGGATCTTCTGATGATAAACGCTTCAATGTCGAACCGAGAAGCTGCTTAAACCAGGAGAGAGTTGCTCGTAGTACACTAACAGAAGAAACACACGCAGATTGTATGCTGTCGGAATCCGAATATCCAAGCCAGAGAAGAGCCGATGCGCTCGAGTCTCTACTCGAGCTATGTGCACGGTTACTCAAGCAAGACAAGCTCGAAGAACTTGCTGGTGTGCTGAGTCCATTCGGAGAAGAAGCGGTATCGTCGAGAGAAACAGCAATCTGGTTAACAAAGAGTCTGATATCGTCGAAATGCAGAATTAAAACGGAGGACCTTAGTGTGATCTAGAATCTATTTGGCTT

mRNA sequence

TCTTCTTTCAAATGGGTTTGTCGACATCTCATCTGCCTGCCCTCAATTTGAAGCTCTTATGAATCTGCTAATCGAAGCAACGCTTTTGGGTTTGCCTTTCCCTTTCATCTTCCTAACTTCCCACTTTCCCTTTCTCTTTTGAGTTTGTCATTCCCTTCACATTCTTTACAACATCATCATAATTTCACGGTTGCTTTACTCATTGATTGTGTCTCTGTAGATTCTGGAAATTCCAGTTATAATTGAAAGACTTATAGCTTTTGGGGGAAGTTTTTGGTTCAAACTTACCCAATTTGGTGTTGGATTTTGATTTTTGTTAAGAAGTTTTCGCCATGACGACATGGAGTGTGATGATGATGATATGAAGAACAGGATGGAAGATTATGAAGTTCTTGAGCAGATTGGGAGGGGAGCTTTTGGATCAGCTTTTCTTGTTTACCATAAGATTGAGAAGAAAAAGTATGTACTCAAGAAAATTCGCTTGGCTAAACAAACGGAGAAGTTCAAGCGCACTGCACACCAGGAGATGAATTTGATTGCAACACTAAATCACCCATATATTGTGGACTACAAAGATTCATGGGTGGATAAGGGTGATTGTATATGCATCGTAACGGGCTATTGCGAAGGAGGAGACATGTCCGAACTGATCAAAAAGGCCAAAGGAACATACTTTCCCGAGGAAAAACTTTGCAAGTGGTTGGCGCAGTTGTTACTAGCTGTGGACTACCTCCACTCGAATCGAGTGCTACATAGAGATCTTAAGTGTTCCAACATTTTCCTTACAAAGGGCAATGATATCAGGCTTGGGGACTTCGGACTTGCAAAACTACTGAATACGGAAGATCTTGCTTCATCGGTTGTTGGTACTCCTAATTACATGTGCCCCGAGCTCCTCACAGATATACCTTATGGCTACAAGTCCGATATATGGTCACTTGGTTGCTGTATGTTTGAAATTTCTGCTCATCAACCTGCTTTTCGAGCTCCGGACATGGCTGGTCTTATCAATAAAATTAACAGATCCTCCATTTCTCCTCTCCCAATTGTTTATTCCTCTGCGTTGAAACAGATCATCAAGAGCATGCTACGGAAGAATCCCGGACACAGACCGACGGCTGCTGAATTATTGAGGCATCCACATATCCAACCGTATGTCCTTCAATGCCGCAACACTTCGGCTAATATACTACCGATATGTCCCGACTCAAAGAATAAAACACCACGAAAATCGCCATCTAACAAGTTGAATAGTGGGCATGAGAGCCGAGGCAAACCGAGCGTAGTTTTCAACCTGCTGGAAAATATCGATCTGGTGGCCGGAAGTGCTGACATACATAATAGCAACTCGTCAACTGATGAAAATATGTCGTTAACAGCTATTTCGGAAGACATACTCGAGACGAAGATGGTCGATCCTACATCCTTTCCTGATGAAATATCCACATCAAATGCTGTTGATACTGATGCGCAAACGAGTTCTGCGTCTGTGACATCGACTTGCAATGGTGGAAACAGCATCCGGTGTACGAATTCTTTGCAGAAAGAACCTCGATTGGAGGACGTACCGAAAACAAGTTGCAACGAACCGGGAACGAAGGCTGAGGAAACTAGTGAAGGGAACCCAATGGAATCATCGATATCCTCCATACGCAGCACTGAAAAGTCCGGATCTTCTGATGATAAACGCTTCAATGTCGAACCGAGAAGCTGCTTAAACCAGGAGAGAGTTGCTCGTAGTACACTAACAGAAGAAACACACGCAGATTGTATGCTGTCGGAATCCGAATATCCAAGCCAGAGAAGAGCCGATGCGCTCGAGTCTCTACTCGAGCTATGTGCACGGTTACTCAAGCAAGACAAGCTCGAAGAACTTGCTGGTGTGCTGAGTCCATTCGGAGAAGAAGCGGTATCGTCGAGAGAAACAGCAATCTGGTTAACAAAGAGTCTGATATCGTCGAAATGCAGAATTAAAACGGAGGACCTTAGTGTGATCTAGAATCTATTTGGCTT

Coding sequence (CDS)

ATGGAGTGTGATGATGATGATATGAAGAACAGGATGGAAGATTATGAAGTTCTTGAGCAGATTGGGAGGGGAGCTTTTGGATCAGCTTTTCTTGTTTACCATAAGATTGAGAAGAAAAAGTATGTACTCAAGAAAATTCGCTTGGCTAAACAAACGGAGAAGTTCAAGCGCACTGCACACCAGGAGATGAATTTGATTGCAACACTAAATCACCCATATATTGTGGACTACAAAGATTCATGGGTGGATAAGGGTGATTGTATATGCATCGTAACGGGCTATTGCGAAGGAGGAGACATGTCCGAACTGATCAAAAAGGCCAAAGGAACATACTTTCCCGAGGAAAAACTTTGCAAGTGGTTGGCGCAGTTGTTACTAGCTGTGGACTACCTCCACTCGAATCGAGTGCTACATAGAGATCTTAAGTGTTCCAACATTTTCCTTACAAAGGGCAATGATATCAGGCTTGGGGACTTCGGACTTGCAAAACTACTGAATACGGAAGATCTTGCTTCATCGGTTGTTGGTACTCCTAATTACATGTGCCCCGAGCTCCTCACAGATATACCTTATGGCTACAAGTCCGATATATGGTCACTTGGTTGCTGTATGTTTGAAATTTCTGCTCATCAACCTGCTTTTCGAGCTCCGGACATGGCTGGTCTTATCAATAAAATTAACAGATCCTCCATTTCTCCTCTCCCAATTGTTTATTCCTCTGCGTTGAAACAGATCATCAAGAGCATGCTACGGAAGAATCCCGGACACAGACCGACGGCTGCTGAATTATTGAGGCATCCACATATCCAACCGTATGTCCTTCAATGCCGCAACACTTCGGCTAATATACTACCGATATGTCCCGACTCAAAGAATAAAACACCACGAAAATCGCCATCTAACAAGTTGAATAGTGGGCATGAGAGCCGAGGCAAACCGAGCGTAGTTTTCAACCTGCTGGAAAATATCGATCTGGTGGCCGGAAGTGCTGACATACATAATAGCAACTCGTCAACTGATGAAAATATGTCGTTAACAGCTATTTCGGAAGACATACTCGAGACGAAGATGGTCGATCCTACATCCTTTCCTGATGAAATATCCACATCAAATGCTGTTGATACTGATGCGCAAACGAGTTCTGCGTCTGTGACATCGACTTGCAATGGTGGAAACAGCATCCGGTGTACGAATTCTTTGCAGAAAGAACCTCGATTGGAGGACGTACCGAAAACAAGTTGCAACGAACCGGGAACGAAGGCTGAGGAAACTAGTGAAGGGAACCCAATGGAATCATCGATATCCTCCATACGCAGCACTGAAAAGTCCGGATCTTCTGATGATAAACGCTTCAATGTCGAACCGAGAAGCTGCTTAAACCAGGAGAGAGTTGCTCGTAGTACACTAACAGAAGAAACACACGCAGATTGTATGCTGTCGGAATCCGAATATCCAAGCCAGAGAAGAGCCGATGCGCTCGAGTCTCTACTCGAGCTATGTGCACGGTTACTCAAGCAAGACAAGCTCGAAGAACTTGCTGGTGTGCTGAGTCCATTCGGAGAAGAAGCGGTATCGTCGAGAGAAACAGCAATCTGGTTAACAAAGAGTCTGATATCGTCGAAATGCAGAATTAAAACGGAGGACCTTAGTGTGATCTAG

Protein sequence

MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKWLAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNYMCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSSALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPSNKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDPTSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTKAEETSEGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCMLSESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISSKCRIKTEDLSVI
Homology
BLAST of Carg22477 vs. NCBI nr
Match: KAG7033550.1 (Serine/threonine-protein kinase Nek6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 552/552 (100.00%), Postives = 552/552 (100.00%), Query Frame = 0

Query: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60
           MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120
           QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW
Sbjct: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180

Query: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240
           MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS
Sbjct: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240

Query: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPS 300
           ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPS
Sbjct: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPS 300

Query: 301 NKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDP 360
           NKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDP
Sbjct: 301 NKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDP 360

Query: 361 TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTK 420
           TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTK
Sbjct: 361 TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTK 420

Query: 421 AEETSEGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCMLS 480
           AEETSEGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCMLS
Sbjct: 421 AEETSEGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCMLS 480

Query: 481 ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS 540
           ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS
Sbjct: 481 ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS 540

Query: 541 KCRIKTEDLSVI 553
           KCRIKTEDLSVI
Sbjct: 541 KCRIKTEDLSVI 552

BLAST of Carg22477 vs. NCBI nr
Match: KAG6603248.1 (Serine/threonine-protein kinase Nek6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1061.6 bits (2744), Expect = 2.3e-306
Identity = 538/541 (99.45%), Postives = 540/541 (99.82%), Query Frame = 0

Query: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60
           MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 54  MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 113

Query: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120
           QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW
Sbjct: 114 QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 173

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY
Sbjct: 174 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 233

Query: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240
           MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS
Sbjct: 234 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 293

Query: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPS 300
           ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPS
Sbjct: 294 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPS 353

Query: 301 NKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDP 360
           NKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDP
Sbjct: 354 NKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDP 413

Query: 361 TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTK 420
           TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTK
Sbjct: 414 TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTK 473

Query: 421 AEETSEGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCMLS 480
           AEETSEGNPMESSISSIRS EKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADC+LS
Sbjct: 474 AEETSEGNPMESSISSIRSAEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCILS 533

Query: 481 ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS 540
           ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS
Sbjct: 534 ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS 593

Query: 541 K 542
           +
Sbjct: 594 Q 594

BLAST of Carg22477 vs. NCBI nr
Match: XP_022928610.1 (serine/threonine-protein kinase Nek6-like [Cucurbita moschata] >XP_022928611.1 serine/threonine-protein kinase Nek6-like [Cucurbita moschata] >XP_022928612.1 serine/threonine-protein kinase Nek6-like [Cucurbita moschata] >XP_022928613.1 serine/threonine-protein kinase Nek6-like [Cucurbita moschata])

HSP 1 Score: 1050.0 bits (2714), Expect = 6.9e-303
Identity = 532/541 (98.34%), Postives = 536/541 (99.08%), Query Frame = 0

Query: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60
           MECDDDDMKNRMEDYEVLEQIGRGAFG AFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGLAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120
           QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW
Sbjct: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180

Query: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240
           MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS
Sbjct: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240

Query: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPS 300
           ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRN SANILPICPDSKNKTPRKSPS
Sbjct: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNASANILPICPDSKNKTPRKSPS 300

Query: 301 NKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDP 360
           NKLNSGHESR KPSVVFNLLEN+DLVAGSADIHNSNSST+ENMSLTAISEDILETKMVDP
Sbjct: 301 NKLNSGHESRAKPSVVFNLLENMDLVAGSADIHNSNSSTEENMSLTAISEDILETKMVDP 360

Query: 361 TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTK 420
           TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNS QKEPRLEDVPKTSCNEPGTK
Sbjct: 361 TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSSQKEPRLEDVPKTSCNEPGTK 420

Query: 421 AEETSEGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCMLS 480
           AEETSEGNPMESSISSIRS EKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADC+LS
Sbjct: 421 AEETSEGNPMESSISSIRSAEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCILS 480

Query: 481 ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS 540
           ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS
Sbjct: 481 ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS 540

Query: 541 K 542
           +
Sbjct: 541 Q 541

BLAST of Carg22477 vs. NCBI nr
Match: XP_023543552.1 (serine/threonine-protein kinase Nek6-like [Cucurbita pepo subsp. pepo] >XP_023543553.1 serine/threonine-protein kinase Nek6-like [Cucurbita pepo subsp. pepo] >XP_023543554.1 serine/threonine-protein kinase Nek6-like [Cucurbita pepo subsp. pepo] >XP_023543555.1 serine/threonine-protein kinase Nek6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.1e-291
Identity = 522/551 (94.74%), Postives = 528/551 (95.83%), Query Frame = 0

Query: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60
           MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120
           QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW
Sbjct: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180

Query: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240
           MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS
Sbjct: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240

Query: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPS 300
           ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRN SANILPICPDSKNKTPRKSPS
Sbjct: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNASANILPICPDSKNKTPRKSPS 300

Query: 301 NKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDP 360
           NKLNSGHESR KPSVVFNLLENIDLVA SADIHNSNSSTDENMSLTAISEDILETKMVDP
Sbjct: 301 NKLNSGHESRAKPSVVFNLLENIDLVARSADIHNSNSSTDENMSLTAISEDILETKMVDP 360

Query: 361 TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLE----DVPKTSCNE 420
           TSFPDEISTSNAVD+DAQTSSASVTSTCNGGNSIRCTNSLQK+PRLE    DVPKTSCN+
Sbjct: 361 TSFPDEISTSNAVDSDAQTSSASVTSTCNGGNSIRCTNSLQKQPRLETVNQDVPKTSCNK 420

Query: 421 PGTKAEETSEGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCLNQE------RVARSTLT 480
           P  KAEETSE N MESSISS+RS EKSGSSDDKRFNVEPRSCLNQE       VARST T
Sbjct: 421 PVMKAEETSERNSMESSISSVRSAEKSGSSDDKRFNVEPRSCLNQESFKGHREVARSTQT 480

Query: 481 EETHADCMLSESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETA 540
           EET AD M SES+YPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETA
Sbjct: 481 EETQADSMSSESKYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETA 540

Query: 541 IWLTKSLISSK 542
           IWLTKSLISS+
Sbjct: 541 IWLTKSLISSQ 551

BLAST of Carg22477 vs. NCBI nr
Match: XP_022967762.1 (serine/threonine-protein kinase Nek6-like [Cucurbita maxima] >XP_022967764.1 serine/threonine-protein kinase Nek6-like [Cucurbita maxima] >XP_022967765.1 serine/threonine-protein kinase Nek6-like [Cucurbita maxima] >XP_022967766.1 serine/threonine-protein kinase Nek6-like [Cucurbita maxima])

HSP 1 Score: 989.2 bits (2556), Expect = 1.4e-284
Identity = 512/546 (93.77%), Postives = 519/546 (95.05%), Query Frame = 0

Query: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60
           MECD+DDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MECDNDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120
           QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW
Sbjct: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180

Query: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240
           MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS
Sbjct: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240

Query: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPS 300
           ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRN SANILPICPDSKNKTP+KSPS
Sbjct: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNASANILPICPDSKNKTPQKSPS 300

Query: 301 NKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDP 360
           NKLNSGHESR KPSVVFNLLENIDLVA SADIHNSNSSTDENMSLTAISEDILETKMVDP
Sbjct: 301 NKLNSGHESRAKPSVVFNLLENIDLVARSADIHNSNSSTDENMSLTAISEDILETKMVDP 360

Query: 361 TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLE----DVPKTSCNE 420
           TSFPDEISTSNAVD+D QTSSASVTSTCNGGNSIR TNSLQKE RLE    +VPKTSCN 
Sbjct: 361 TSFPDEISTSNAVDSDVQTSSASVTSTCNGGNSIRRTNSLQKELRLETVNQEVPKTSCNR 420

Query: 421 PGTKAEETSEGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCL-NQERVARSTLTEETHA 480
           PG KAEETSE N MESSISS+R  EKSGSSDDKRFNVEPRS     + VA S  TEETHA
Sbjct: 421 PGMKAEETSEANSMESSISSVR--EKSGSSDDKRFNVEPRSSFKGHQEVAHSAQTEETHA 480

Query: 481 DCMLSESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTK 540
           DCM  ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTK
Sbjct: 481 DCMSLESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTK 540

Query: 541 SLISSK 542
           SLISS+
Sbjct: 541 SLISSQ 544

BLAST of Carg22477 vs. ExPASy Swiss-Prot
Match: Q9LT35 (Serine/threonine-protein kinase Nek6 OS=Arabidopsis thaliana OX=3702 GN=NEK6 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 5.6e-138
Identity = 275/540 (50.93%), Postives = 338/540 (62.59%), Query Frame = 0

Query: 12  MEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIATLNH 71
           M+DYEV+EQIGRGAFGSAFLV HK E++KYV+KKIRLAKQTE+ K  A QEM+LI+ L  
Sbjct: 1   MDDYEVVEQIGRGAFGSAFLVIHKSERRKYVVKKIRLAKQTERCKLAAIQEMSLISKLKS 60

Query: 72  PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKWLAQLLLAVDYL 131
           PYIV+YKDSWV+K DC+CIVT YCEGGDM+++IKK++G +  EEKLC+W+ QLLLA+DYL
Sbjct: 61  PYIVEYKDSWVEK-DCVCIVTSYCEGGDMTQMIKKSRGVFASEEKLCRWMVQLLLAIDYL 120

Query: 132 HSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNYMCPELLTDIPY 191
           H+NRVLHRDLKCSNIFLTK N++RLGDFGLAKLL  +DLASS+VGTPNYMCPELL DIPY
Sbjct: 121 HNNRVLHRDLKCSNIFLTKENEVRLGDFGLAKLLGKDDLASSMVGTPNYMCPELLADIPY 180

Query: 192 GYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSSALKQIIKSMLR 251
           GYKSDIWSLGCCMFE++AHQPAF+APDMA LINKINRSS+SPLP++YSS+LK++IKSMLR
Sbjct: 181 GYKSDIWSLGCCMFEVAAHQPAFKAPDMAALINKINRSSLSPLPVMYSSSLKRLIKSMLR 240

Query: 252 KNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPSNKLNSGHESRG 311
           KNP HRPTAAELLRHPH+QPY+ QC+N S    P+   S+                    
Sbjct: 241 KNPEHRPTAAELLRHPHLQPYLAQCQNLSPVFKPVVSKSE-------------------- 300

Query: 312 KPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDPTSFPDEISTSN 371
                                HN+N +                      T  P       
Sbjct: 301 ---------------------HNTNENR---------------------TGLP------- 360

Query: 372 AVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTKAEETSEGNPME 431
                                                        P TK+ +T   +  E
Sbjct: 361 ---------------------------------------------PKTKSAKTPIKHNQE 416

Query: 432 SSISSIRSTEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCMLSESEYPSQRRAD 491
           S  +  ++ + S SS DK      R   +QE    STLT          +    S+ RA+
Sbjct: 421 SEETEKKNKDTSSSSKDKE-----RPAKSQEMSVISTLTLLRE----FQKKSPKSEERAE 416

Query: 492 ALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISSKCRIKTEDLSV 551
           ALESLLELCA LL+Q+K +EL GVL PFG+E VSSRETAIWLTKSL++ K +   ++ ++
Sbjct: 481 ALESLLELCAGLLRQEKFDELEGVLKPFGDETVSSRETAIWLTKSLMNVKRKQNDDETNI 416

BLAST of Carg22477 vs. ExPASy Swiss-Prot
Match: Q6YY75 (Serine/threonine-protein kinase Nek6 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK6 PE=1 SV=2)

HSP 1 Score: 490.3 bits (1261), Expect = 2.8e-137
Identity = 281/536 (52.43%), Postives = 366/536 (68.28%), Query Frame = 0

Query: 12  MEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIATLNH 71
           ME YEV+EQIGRGA+GSA+LV HK E+K+YV+KKIRL+KQ +KF+RTA+QEM+L+A+L++
Sbjct: 1   MEQYEVVEQIGRGAYGSAYLVVHKGERKRYVMKKIRLSKQNDKFQRTAYQEMSLMASLSN 60

Query: 72  PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKWLAQLLLAVDYL 131
           PYIV+YKD WVD+G   CIVT YCEGGDM+E IKKA+G  F EE++C+W  QLLLA+DYL
Sbjct: 61  PYIVEYKDGWVDEGTSACIVTSYCEGGDMAERIKKARGVLFSEERVCRWFTQLLLALDYL 120

Query: 132 HSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNYMCPELLTDIPY 191
           H NRVLHRDLKCSNI LTK N+IRL DFGLAKLL  EDLAS++VGTPNYMCPE+L DIPY
Sbjct: 121 HCNRVLHRDLKCSNILLTKDNNIRLADFGLAKLL-MEDLASTIVGTPNYMCPEILADIPY 180

Query: 192 GYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSSALKQIIKSMLR 251
           GYKSDIWSLGCCMFEI AH+PAF+A DMA LINKINRSSISP+P +YSS+LKQI+KSMLR
Sbjct: 181 GYKSDIWSLGCCMFEILAHRPAFKAADMASLINKINRSSISPMPPIYSSSLKQIVKSMLR 240

Query: 252 KNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPSNKLNSGHESRG 311
           KNP HRPTA ELLRHP++QPY+ +  + S   LP+ P +K+    K  S K  SG +   
Sbjct: 241 KNPEHRPTAGELLRHPYLQPYLAESCSCSPIYLPVKP-TKSNLGDKQQSRKPGSGRKRII 300

Query: 312 KPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDPTSFP------D 371
           K +      E ++  A  A     NS+   ++S     +  +    VDP +        D
Sbjct: 301 KTN---GSSEALETAAEQAVDTRDNSTYISDVSTVGTQDACISQVSVDPQARNKAYQNID 360

Query: 372 EISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTKAEETS 431
           +++    ++ +  T +           ++R ++++   P    + K   NE     EE +
Sbjct: 361 DLTLFQQIEENLMTITDRQIDEAIFLKAVRTSSTVDVVPVSGAIQKP--NEAPIPKEELT 420

Query: 432 EGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCMLSESEYP 491
            G   E      R   K+ +    +        + +E   +S + +  H+D   +E ++ 
Sbjct: 421 IGVVQEQ-----RKEVKAHTHQGSKPGTGDVPIVTEESSPKSAV-KLAHSDSTPAEWDHL 480

Query: 492 S--QRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLIS 540
           +  Q+RADALESLLELCA+LLKQ++LEELAGVL PFGE AVSSRETAIWLTKSL++
Sbjct: 481 NIVQQRADALESLLELCAKLLKQERLEELAGVLRPFGEGAVSSRETAIWLTKSLMT 523

BLAST of Carg22477 vs. ExPASy Swiss-Prot
Match: Q9LHI7 (Serine/threonine-protein kinase Nek7 OS=Arabidopsis thaliana OX=3702 GN=NEK7 PE=2 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.6e-124
Identity = 276/570 (48.42%), Postives = 361/570 (63.33%), Query Frame = 0

Query: 1   MECDD--DDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRT 60
           ME +D  ++ K  +++Y V+EQ+ RG   S F+V H IE KKY +KKI LAK T+K K+T
Sbjct: 3   MEANDCQEEHKFTLDNYHVVEQVRRGKSSSDFVVLHDIEDKKYAMKKICLAKHTDKLKQT 62

Query: 61  AHQEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLC 120
           A QEM L+++L +PYIV Y+DSW+D  +  CI T Y EGG+M+  IKKA+G  FPEE++ 
Sbjct: 63  ALQEMKLLSSLKNPYIVHYEDSWIDNDNNACIFTAYYEGGNMANAIKKARGKLFPEERIF 122

Query: 121 KWLAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTP 180
           KWLAQLLLAV+YLHSNRV+H DL CSNIFL K + ++LG++GLAKL+N E   S V G  
Sbjct: 123 KWLAQLLLAVNYLHSNRVVHMDLTCSNIFLPKDDHVQLGNYGLAKLINPEKPVSMVSGIS 182

Query: 181 NYMCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVY 240
           N MCPE+L D PYGYKSDIWSLGCCM+EI+AHQPAF+APDMAGLINKINRS +SPLPIVY
Sbjct: 183 NSMCPEVLEDQPYGYKSDIWSLGCCMYEITAHQPAFKAPDMAGLINKINRSLMSPLPIVY 242

Query: 241 SSALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICP-----DSKNK 300
           SS LKQ+IK MLRK P +RPTA ELLR+P +QPY+LQC+N S   LP+ P       K+K
Sbjct: 243 SSTLKQMIKLMLRKKPEYRPTACELLRNPSLQPYLLQCQNLSPIYLPVFPIKPVNSPKDK 302

Query: 301 TPRKSPSNKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDIL 360
             R S   K      SR K S V   LEN  L     +    +SS+ +  S T  +ED L
Sbjct: 303 ARRNSLPGKFGKERVSREK-SEVSRSLEN--LYPFWTNTETGSSSSSQPASSTNGAEDKL 362

Query: 361 ETKMVDP-------TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRL 420
           ETK +DP       + F  + S  + +D D    S    +  N        N   +E +L
Sbjct: 363 ETKRIDPSCDTLKISEFTSQKSDESLIDPDIAVYSTETPAEENALPK-ETENIFSEESQL 422

Query: 421 EDV-------PKTSCNEPGTKAE-ETSEGNPMESS-----ISSI--RSTEKSGSSDDKRF 480
            DV        + +C+ P    E ET E  P         ISS+   STE + + D    
Sbjct: 423 RDVDVGVVSAQEVACSPPRAIEEAETQEALPKPKEQITVPISSVAHSSTEVAAAKDHLSG 482

Query: 481 NVEPRSCLNQERVARSTLTEETHADCMLSESEYP-SQRRADALESLLELCARLLKQDKLE 540
           ++E     ++ ++ + T +E +     L++   P S+ RADALE LLE CA L+KQ+K E
Sbjct: 483 SLEG----DKAKMVKLTASEMSSVLSKLTKLGPPQSKERADALECLLEKCAGLVKQEKYE 542

BLAST of Carg22477 vs. ExPASy Swiss-Prot
Match: A2ZMH2 (Serine/threonine-protein kinase Nek2 OS=Oryza sativa subsp. indica OX=39946 GN=NEK2 PE=3 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 1.2e-121
Identity = 272/592 (45.95%), Postives = 357/592 (60.30%), Query Frame = 0

Query: 12  MEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIATLNH 71
           M+ YEVLEQIG+GAFGSA LV HK+EKKKYVLKKIRLA+QT++ +R+AHQEM LIAT+ +
Sbjct: 1   MDQYEVLEQIGKGAFGSALLVRHKLEKKKYVLKKIRLARQTDRTRRSAHQEMQLIATVRN 60

Query: 72  PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKWLAQLLLAVDYL 131
           P+IV+YKDSWV+KG  +CIV GYCEGGDM+E IK+A GTYF EEKLCKWL QLL+A+DYL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIVIGYCEGGDMAEAIKRANGTYFSEEKLCKWLVQLLMALDYL 120

Query: 132 HSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNYMCPELLTDIPY 191
           H+N +LHRD+KCSNIF+ +   IRLGDFGLAK+L ++DLASSVVGTP+YMCPELL DIPY
Sbjct: 121 HANHILHRDVKCSNIFIARDQSIRLGDFGLAKILTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 192 GYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSSALKQIIKSMLR 251
           G KSDIWSLGCC++E++A +PAF+A DM  LINKI +S +SPLP  YS A + +IKSMLR
Sbjct: 181 GTKSDIWSLGCCIYEMTALRPAFKAFDMQALINKITKSIVSPLPTKYSGAFRGLIKSMLR 240

Query: 252 KNPGHRPTAAELLRHPHIQPYVLQCRNTSA---NILPICPDSKNKTPRKS-PSNKLNSGH 311
           K+P HRP+AA+LL+HP +QPYVLQ +  S+   NILPI     +K  + + PS+ ++S  
Sbjct: 241 KSPEHRPSAAQLLKHPQLQPYVLQVQLKSSPTRNILPIHQSLTDKVKKMTFPSDVVDSAR 300

Query: 312 ESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDPTSFPDEI 371
               + + + N  E     +  +   NS SST      T      +E   VD +   DE+
Sbjct: 301 RRMARRNSLGN--ERTVTFSKPSPERNSVSSTRSIKEYTTTQS--VEGLSVDSSEAGDEV 360

Query: 372 STSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQ-------KEPRLEDVPKTSCNEPGTK 431
            TS A+ T  +TSS   T       +    N L+       +  R E   KT+ N+    
Sbjct: 361 -TSKAIIT--KTSSILRTPKSLPAKTYTARNQLEPPKTSYNRTYRSELPSKTTPNKIARP 420

Query: 432 AEETS-----EGNPMESSISSIRSTEKS-----------------GSSDDKRFNVEPR-- 491
           A   S        P + SIS +   +                    SS+D    +  +  
Sbjct: 421 ARRASLPLSTYETPTKRSISILEQLDSPDVSVNAPRIDRIAEFPLASSEDPLLPIHNKLS 480

Query: 492 ----SCLNQERVARSTLTEETHADCMLSESEYPS-------------------------- 537
               SC     + RS   ++     + ++ +  S                          
Sbjct: 481 PGHGSCSTPPFINRSITKDKCTIQVLRTDGDNGSDSSGRNATAASSRGSNDSRQQRFDTS 540

BLAST of Carg22477 vs. ExPASy Swiss-Prot
Match: Q2QMH1 (Serine/threonine-protein kinase Nek2 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK2 PE=2 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 3.6e-121
Identity = 271/592 (45.78%), Postives = 357/592 (60.30%), Query Frame = 0

Query: 12  MEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIATLNH 71
           M+ YEVLEQIG+GAFGSA LV HK+EKKKYVLKKIRLA+QT++ +R+AHQEM LIAT+ +
Sbjct: 1   MDQYEVLEQIGKGAFGSALLVRHKLEKKKYVLKKIRLARQTDRTRRSAHQEMQLIATVRN 60

Query: 72  PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKWLAQLLLAVDYL 131
           P+IV+YKDSWV+KG  +CIV GYCEGGDM+E IK+A GTYF EEKLCKWL QLL+A+DYL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIVIGYCEGGDMAEAIKRANGTYFSEEKLCKWLVQLLMALDYL 120

Query: 132 HSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNYMCPELLTDIPY 191
           H+N +LHRD+KCSNIF+ +   IRLGDFGLAK+L ++DLASSVVGTP+YMCPELL DIPY
Sbjct: 121 HANHILHRDVKCSNIFIARDQSIRLGDFGLAKILTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 192 GYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSSALKQIIKSMLR 251
           G KSDIWSLGCC++E++A +PAF+A DM  LINKI +S +SPLP  YS A + +IKSMLR
Sbjct: 181 GTKSDIWSLGCCIYEMTALRPAFKAFDMQALINKITKSIVSPLPTKYSGAFRGLIKSMLR 240

Query: 252 KNPGHRPTAAELLRHPHIQPYVLQCRNTSA---NILPICPDSKNKTPRKS-PSNKLNSGH 311
           K+P HRP+AA+LL+HP +QPYVLQ +  S+   NILPI     +K  + + PS+ ++S  
Sbjct: 241 KSPEHRPSAAQLLKHPQLQPYVLQVQLKSSPTRNILPIHQSLTDKVKKMTFPSDVVDSAR 300

Query: 312 ESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDPTSFPDEI 371
               + + + N  E     +  +   NS SST      T      ++   VD +   DE+
Sbjct: 301 RRMARRNSLGN--ERTVTFSKPSPERNSVSSTRSIKEYTTTQS--VKGLSVDSSEAGDEV 360

Query: 372 STSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQ-------KEPRLEDVPKTSCNEPGTK 431
            TS A+ T  +TSS   T       +    N L+       +  R E   KT+ N+    
Sbjct: 361 -TSKAIIT--KTSSILRTPKSLPAKTYTARNQLEPPKTSYNRTYRSELPSKTTPNKIARP 420

Query: 432 AEETS-----EGNPMESSISSIRSTEKS-----------------GSSDDKRFNVEPR-- 491
           A   S        P + SIS +   +                    SS+D    +  +  
Sbjct: 421 ARRASLPLSTYETPTKRSISILEQLDSPDVSVNAPRIDRIAEFPLASSEDPLLPIHNKLS 480

Query: 492 ----SCLNQERVARSTLTEETHADCMLSESEYPS-------------------------- 537
               SC     + RS   ++     + ++ +  S                          
Sbjct: 481 PGHGSCSTPPFINRSITKDKCTIQVLRTDGDNGSDSSGRNATAASSRGSNDSRQQRFDTS 540

BLAST of Carg22477 vs. ExPASy TrEMBL
Match: A0A6J1EKS2 (serine/threonine-protein kinase Nek6-like OS=Cucurbita moschata OX=3662 GN=LOC111435469 PE=4 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.4e-303
Identity = 532/541 (98.34%), Postives = 536/541 (99.08%), Query Frame = 0

Query: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60
           MECDDDDMKNRMEDYEVLEQIGRGAFG AFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGLAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120
           QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW
Sbjct: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180

Query: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240
           MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS
Sbjct: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240

Query: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPS 300
           ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRN SANILPICPDSKNKTPRKSPS
Sbjct: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNASANILPICPDSKNKTPRKSPS 300

Query: 301 NKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDP 360
           NKLNSGHESR KPSVVFNLLEN+DLVAGSADIHNSNSST+ENMSLTAISEDILETKMVDP
Sbjct: 301 NKLNSGHESRAKPSVVFNLLENMDLVAGSADIHNSNSSTEENMSLTAISEDILETKMVDP 360

Query: 361 TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTK 420
           TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNS QKEPRLEDVPKTSCNEPGTK
Sbjct: 361 TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSSQKEPRLEDVPKTSCNEPGTK 420

Query: 421 AEETSEGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCMLS 480
           AEETSEGNPMESSISSIRS EKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADC+LS
Sbjct: 421 AEETSEGNPMESSISSIRSAEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCILS 480

Query: 481 ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS 540
           ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS
Sbjct: 481 ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISS 540

Query: 541 K 542
           +
Sbjct: 541 Q 541

BLAST of Carg22477 vs. ExPASy TrEMBL
Match: A0A6J1HRP8 (serine/threonine-protein kinase Nek6-like OS=Cucurbita maxima OX=3661 GN=LOC111467187 PE=4 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 7.0e-285
Identity = 512/546 (93.77%), Postives = 519/546 (95.05%), Query Frame = 0

Query: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60
           MECD+DDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MECDNDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120
           QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW
Sbjct: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180

Query: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240
           MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS
Sbjct: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240

Query: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPS 300
           ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRN SANILPICPDSKNKTP+KSPS
Sbjct: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNASANILPICPDSKNKTPQKSPS 300

Query: 301 NKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDP 360
           NKLNSGHESR KPSVVFNLLENIDLVA SADIHNSNSSTDENMSLTAISEDILETKMVDP
Sbjct: 301 NKLNSGHESRAKPSVVFNLLENIDLVARSADIHNSNSSTDENMSLTAISEDILETKMVDP 360

Query: 361 TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLE----DVPKTSCNE 420
           TSFPDEISTSNAVD+D QTSSASVTSTCNGGNSIR TNSLQKE RLE    +VPKTSCN 
Sbjct: 361 TSFPDEISTSNAVDSDVQTSSASVTSTCNGGNSIRRTNSLQKELRLETVNQEVPKTSCNR 420

Query: 421 PGTKAEETSEGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCL-NQERVARSTLTEETHA 480
           PG KAEETSE N MESSISS+R  EKSGSSDDKRFNVEPRS     + VA S  TEETHA
Sbjct: 421 PGMKAEETSEANSMESSISSVR--EKSGSSDDKRFNVEPRSSFKGHQEVAHSAQTEETHA 480

Query: 481 DCMLSESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTK 540
           DCM  ESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTK
Sbjct: 481 DCMSLESEYPSQRRADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTK 540

Query: 541 SLISSK 542
           SLISS+
Sbjct: 541 SLISSQ 544

BLAST of Carg22477 vs. ExPASy TrEMBL
Match: A0A1S3B618 (serine/threonine-protein kinase Nek6 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103486166 PE=4 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 9.6e-234
Identity = 470/683 (68.81%), Postives = 491/683 (71.89%), Query Frame = 0

Query: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60
           ME D+ DMK RMEDYEV+EQIGRGAFGSAFLVYHK EKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120
           QEMNLIA LN+PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKA+GTYFPEEKLCKW
Sbjct: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180

Query: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240
           MCPELL DIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS
Sbjct: 181 MCPELLADIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240

Query: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICP---DSKNKTPRK 300
            LKQIIKSMLRKNP HRPTAAELLRHPH+QPYVLQCRN SANILPI P   +SK+KTPRK
Sbjct: 241 TLKQIIKSMLRKNPEHRPTAAELLRHPHMQPYVLQCRNASANILPIYPLPINSKDKTPRK 300

Query: 301 SPSNKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKM 360
           SPSNKLN GHE R KPS+ FNLLENIDLV GS DIHNSNSSTDENMSLTAISEDILETKM
Sbjct: 301 SPSNKLNGGHEIRAKPSIAFNLLENIDLVGGSGDIHNSNSSTDENMSLTAISEDILETKM 360

Query: 361 VDPTSFPDEISTSNAVD--TDAQTSSASVTSTC-----NGGNSIRCT------------- 420
           VDPTSFPDEISTS+AVD  TD  TSS S T T      NGG +++CT             
Sbjct: 361 VDPTSFPDEISTSDAVDSRTDGLTSSLSPTLTSNGDKENGGIALQCTEPPAIKISPENSP 420

Query: 421 NSLQKEPRLEDV---------------PKTSC-----NEPGTKAEETSEGNPMESSISSI 480
           N LQ+EP  E+                PK SC     N  G K EETSE N +E  +SS+
Sbjct: 421 NYLQREPDAENFQQLREVDIKTVNLHEPKISCSKQTSNVTGMKPEETSERN-VELLMSSV 480

Query: 481 RSTEKSGSSDDK-------RFNVEPRSCLNQERVARS---------TLTEET-------- 540
            S EK G SDDK       + NVE RSC+NQE              T  EET        
Sbjct: 481 GSAEKFGPSDDKCLLNSADQLNVELRSCINQESFKEHPEAGCSSCLTSAEETTGMELVSS 540

Query: 541 ----------------HADCMLS------------------------------------- 542
                           H DCMLS                                     
Sbjct: 541 EKESVQTDCLPLEKGSHTDCMLSEITSNILPRKDEIHVNSDGTSCSTQMITNGNVHQHNR 600

BLAST of Carg22477 vs. ExPASy TrEMBL
Match: A0A5D3E248 (Serine/threonine-protein kinase Nek6 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold208G001040 PE=4 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 9.6e-234
Identity = 470/683 (68.81%), Postives = 491/683 (71.89%), Query Frame = 0

Query: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60
           ME D+ DMK RMEDYEV+EQIGRGAFGSAFLVYHK EKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 111 MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 170

Query: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120
           QEMNLIA LN+PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKA+GTYFPEEKLCKW
Sbjct: 171 QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 230

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY
Sbjct: 231 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 290

Query: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240
           MCPELL DIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS
Sbjct: 291 MCPELLADIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 350

Query: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICP---DSKNKTPRK 300
            LKQIIKSMLRKNP HRPTAAELLRHPH+QPYVLQCRN SANILPI P   +SK+KTPRK
Sbjct: 351 TLKQIIKSMLRKNPEHRPTAAELLRHPHMQPYVLQCRNASANILPIYPLPINSKDKTPRK 410

Query: 301 SPSNKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKM 360
           SPSNKLN GHE R KPS+ FNLLENIDLV GS DIHNSNSSTDENMSLTAISEDILETKM
Sbjct: 411 SPSNKLNGGHEIRAKPSIAFNLLENIDLVGGSGDIHNSNSSTDENMSLTAISEDILETKM 470

Query: 361 VDPTSFPDEISTSNAVD--TDAQTSSASVTSTC-----NGGNSIRCT------------- 420
           VDPTSFPDEISTS+AVD  TD  TSS S T T      NGG +++CT             
Sbjct: 471 VDPTSFPDEISTSDAVDSRTDGLTSSLSPTLTSNGDKENGGIALQCTEPPAIKISPENSP 530

Query: 421 NSLQKEPRLEDV---------------PKTSC-----NEPGTKAEETSEGNPMESSISSI 480
           N LQ+EP  E+                PK SC     N  G K EETSE N +E  +SS+
Sbjct: 531 NYLQREPDAENFQQLREVDIETVNLHEPKISCSKQTSNVTGMKPEETSERN-VELLMSSV 590

Query: 481 RSTEKSGSSDDK-------RFNVEPRSCLNQERVARS---------TLTEET-------- 540
            S EK G SDDK       + NVE RSC+NQE              T  EET        
Sbjct: 591 GSAEKFGPSDDKCLLNSADQLNVELRSCINQESFKEHPEAGCSSCLTSAEETTGMELVSS 650

Query: 541 ----------------HADCMLS------------------------------------- 542
                           H DCMLS                                     
Sbjct: 651 EKESVQTDCLPLEKGSHTDCMLSEITSNILPRKDEIHVNSDGTSCSTQMITNGNVHQHNR 710

BLAST of Carg22477 vs. ExPASy TrEMBL
Match: A0A5A7T2B9 (Serine/threonine-protein kinase Nek6 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold403G00350 PE=4 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 9.6e-234
Identity = 470/683 (68.81%), Postives = 491/683 (71.89%), Query Frame = 0

Query: 1   MECDDDDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAH 60
           ME D+ DMK RMEDYEV+EQIGRGAFGSAFLVYHK EKKKYVLKKIRLAKQTEKFKRTAH
Sbjct: 1   MESDNGDMKTRMEDYEVIEQIGRGAFGSAFLVYHKTEKKKYVLKKIRLAKQTEKFKRTAH 60

Query: 61  QEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKW 120
           QEMNLIA LN+PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKA+GTYFPEEKLCKW
Sbjct: 61  QEMNLIAKLNNPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKARGTYFPEEKLCKW 120

Query: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180
           LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY
Sbjct: 121 LAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNY 180

Query: 181 MCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240
           MCPELL DIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS
Sbjct: 181 MCPELLADIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSS 240

Query: 241 ALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICP---DSKNKTPRK 300
            LKQIIKSMLRKNP HRPTAAELLRHPH+QPYVLQCRN SANILPI P   +SK+KTPRK
Sbjct: 241 TLKQIIKSMLRKNPEHRPTAAELLRHPHMQPYVLQCRNASANILPIYPLPINSKDKTPRK 300

Query: 301 SPSNKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKM 360
           SPSNKLN GHE R KPS+ FNLLENIDLV GS DIHNSNSSTDENMSLTAISEDILETKM
Sbjct: 301 SPSNKLNGGHEIRAKPSIAFNLLENIDLVGGSGDIHNSNSSTDENMSLTAISEDILETKM 360

Query: 361 VDPTSFPDEISTSNAVD--TDAQTSSASVTSTC-----NGGNSIRCT------------- 420
           VDPTSFPDEISTS+AVD  TD  TSS S T T      NGG +++CT             
Sbjct: 361 VDPTSFPDEISTSDAVDSRTDGLTSSLSPTLTSNGDKENGGIALQCTEPPAIKISPENSP 420

Query: 421 NSLQKEPRLEDV---------------PKTSC-----NEPGTKAEETSEGNPMESSISSI 480
           N LQ+EP  E+                PK SC     N  G K EETSE N +E  +SS+
Sbjct: 421 NYLQREPDAENFQQLREVDIKTVNLHEPKISCSKQTSNVTGMKPEETSERN-VELLMSSV 480

Query: 481 RSTEKSGSSDDK-------RFNVEPRSCLNQERVARS---------TLTEET-------- 540
            S EK G SDDK       + NVE RSC+NQE              T  EET        
Sbjct: 481 GSAEKFGPSDDKCLLNSADQLNVELRSCINQESFKEHPEAGCSSCLTSAEETTGMELVSS 540

Query: 541 ----------------HADCMLS------------------------------------- 542
                           H DCMLS                                     
Sbjct: 541 EKESVQTDCLPLEKGSHTDCMLSEITSNILPRKDEIHVNSDGTSCSTQMITNGNVHQHNR 600

BLAST of Carg22477 vs. TAIR 10
Match: AT3G20860.1 (NIMA-related kinase 5 )

HSP 1 Score: 493.8 bits (1270), Expect = 1.8e-139
Identity = 275/542 (50.74%), Postives = 340/542 (62.73%), Query Frame = 0

Query: 10  NRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIATL 69
           ++M+DYEV+EQIGRGAFGSAFLV HK E++KYV+KKIRLAKQTE+ K  A QEM+LI+ L
Sbjct: 10  SKMDDYEVVEQIGRGAFGSAFLVIHKSERRKYVVKKIRLAKQTERCKLAAIQEMSLISKL 69

Query: 70  NHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKWLAQLLLAVD 129
             PYIV+YKDSWV+K DC+CIVT YCEGGDM+++IKK++G +  EEKLC+W+ QLLLA+D
Sbjct: 70  KSPYIVEYKDSWVEK-DCVCIVTSYCEGGDMTQMIKKSRGVFASEEKLCRWMVQLLLAID 129

Query: 130 YLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNYMCPELLTDI 189
           YLH+NRVLHRDLKCSNIFLTK N++RLGDFGLAKLL  +DLASS+VGTPNYMCPELL DI
Sbjct: 130 YLHNNRVLHRDLKCSNIFLTKENEVRLGDFGLAKLLGKDDLASSMVGTPNYMCPELLADI 189

Query: 190 PYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSSALKQIIKSM 249
           PYGYKSDIWSLGCCMFE++AHQPAF+APDMA LINKINRSS+SPLP++YSS+LK++IKSM
Sbjct: 190 PYGYKSDIWSLGCCMFEVAAHQPAFKAPDMAALINKINRSSLSPLPVMYSSSLKRLIKSM 249

Query: 250 LRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICPDSKNKTPRKSPSNKLNSGHES 309
           LRKNP HRPTAAELLRHPH+QPY+ QC+N S    P+   S+                  
Sbjct: 250 LRKNPEHRPTAAELLRHPHLQPYLAQCQNLSPVFKPVVSKSE------------------ 309

Query: 310 RGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDPTSFPDEIST 369
                                  HN+N +                      T  P     
Sbjct: 310 -----------------------HNTNENR---------------------TGLP----- 369

Query: 370 SNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTKAEETSEGNP 429
                                                          P TK+ +T   + 
Sbjct: 370 -----------------------------------------------PKTKSAKTPIKHN 427

Query: 430 MESSISSIRSTEKSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCMLSESEYPSQRR 489
            ES  +  ++ + S SS DK      R   +QE    STLT          +    S+ R
Sbjct: 430 QESEETEKKNKDTSSSSKDKE-----RPAKSQEMSVISTLTLLRE----FQKKSPKSEER 427

Query: 490 ADALESLLELCARLLKQDKLEELAGVLSPFGEEAVSSRETAIWLTKSLISSKCRIKTEDL 549
           A+ALESLLELCA LL+Q+K +EL GVL PFG+E VSSRETAIWLTKSL++ K +   ++ 
Sbjct: 490 AEALESLLELCAGLLRQEKFDELEGVLKPFGDETVSSRETAIWLTKSLMNVKRKQNDDET 427

Query: 550 SV 552
           ++
Sbjct: 550 NI 427

BLAST of Carg22477 vs. TAIR 10
Match: AT3G12200.1 (NIMA-related kinase 7 )

HSP 1 Score: 448.0 bits (1151), Expect = 1.1e-125
Identity = 276/570 (48.42%), Postives = 361/570 (63.33%), Query Frame = 0

Query: 1   MECDD--DDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRT 60
           ME +D  ++ K  +++Y V+EQ+ RG   S F+V H IE KKY +KKI LAK T+K K+T
Sbjct: 3   MEANDCQEEHKFTLDNYHVVEQVRRGKSSSDFVVLHDIEDKKYAMKKICLAKHTDKLKQT 62

Query: 61  AHQEMNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLC 120
           A QEM L+++L +PYIV Y+DSW+D  +  CI T Y EGG+M+  IKKA+G  FPEE++ 
Sbjct: 63  ALQEMKLLSSLKNPYIVHYEDSWIDNDNNACIFTAYYEGGNMANAIKKARGKLFPEERIF 122

Query: 121 KWLAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTP 180
           KWLAQLLLAV+YLHSNRV+H DL CSNIFL K + ++LG++GLAKL+N E   S V G  
Sbjct: 123 KWLAQLLLAVNYLHSNRVVHMDLTCSNIFLPKDDHVQLGNYGLAKLINPEKPVSMVSGIS 182

Query: 181 NYMCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVY 240
           N MCPE+L D PYGYKSDIWSLGCCM+EI+AHQPAF+APDMAGLINKINRS +SPLPIVY
Sbjct: 183 NSMCPEVLEDQPYGYKSDIWSLGCCMYEITAHQPAFKAPDMAGLINKINRSLMSPLPIVY 242

Query: 241 SSALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICP-----DSKNK 300
           SS LKQ+IK MLRK P +RPTA ELLR+P +QPY+LQC+N S   LP+ P       K+K
Sbjct: 243 SSTLKQMIKLMLRKKPEYRPTACELLRNPSLQPYLLQCQNLSPIYLPVFPIKPVNSPKDK 302

Query: 301 TPRKSPSNKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDIL 360
             R S   K      SR K S V   LEN  L     +    +SS+ +  S T  +ED L
Sbjct: 303 ARRNSLPGKFGKERVSREK-SEVSRSLEN--LYPFWTNTETGSSSSSQPASSTNGAEDKL 362

Query: 361 ETKMVDP-------TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRL 420
           ETK +DP       + F  + S  + +D D    S    +  N        N   +E +L
Sbjct: 363 ETKRIDPSCDTLKISEFTSQKSDESLIDPDIAVYSTETPAEENALPK-ETENIFSEESQL 422

Query: 421 EDV-------PKTSCNEPGTKAE-ETSEGNPMESS-----ISSI--RSTEKSGSSDDKRF 480
            DV        + +C+ P    E ET E  P         ISS+   STE + + D    
Sbjct: 423 RDVDVGVVSAQEVACSPPRAIEEAETQEALPKPKEQITVPISSVAHSSTEVAAAKDHLSG 482

Query: 481 NVEPRSCLNQERVARSTLTEETHADCMLSESEYP-SQRRADALESLLELCARLLKQDKLE 540
           ++E     ++ ++ + T +E +     L++   P S+ RADALE LLE CA L+KQ+K E
Sbjct: 483 SLEG----DKAKMVKLTASEMSSVLSKLTKLGPPQSKERADALECLLEKCAGLVKQEKYE 542

BLAST of Carg22477 vs. TAIR 10
Match: AT3G12200.2 (NIMA-related kinase 7 )

HSP 1 Score: 439.9 bits (1130), Expect = 3.1e-123
Identity = 276/580 (47.59%), Postives = 361/580 (62.24%), Query Frame = 0

Query: 1   MECDD--DDMKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRT 60
           ME +D  ++ K  +++Y V+EQ+ RG   S F+V H IE KKY +KKI LAK T+K K+T
Sbjct: 3   MEANDCQEEHKFTLDNYHVVEQVRRGKSSSDFVVLHDIEDKKYAMKKICLAKHTDKLKQT 62

Query: 61  AHQE----------MNLIATLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAK 120
           A QE          M L+++L +PYIV Y+DSW+D  +  CI T Y EGG+M+  IKKA+
Sbjct: 63  ALQEISRAVINYDLMKLLSSLKNPYIVHYEDSWIDNDNNACIFTAYYEGGNMANAIKKAR 122

Query: 121 GTYFPEEKLCKWLAQLLLAVDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTE 180
           G  FPEE++ KWLAQLLLAV+YLHSNRV+H DL CSNIFL K + ++LG++GLAKL+N E
Sbjct: 123 GKLFPEERIFKWLAQLLLAVNYLHSNRVVHMDLTCSNIFLPKDDHVQLGNYGLAKLINPE 182

Query: 181 DLASSVVGTPNYMCPELLTDIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINR 240
              S V G  N MCPE+L D PYGYKSDIWSLGCCM+EI+AHQPAF+APDMAGLINKINR
Sbjct: 183 KPVSMVSGISNSMCPEVLEDQPYGYKSDIWSLGCCMYEITAHQPAFKAPDMAGLINKINR 242

Query: 241 SSISPLPIVYSSALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILPICP 300
           S +SPLPIVYSS LKQ+IK MLRK P +RPTA ELLR+P +QPY+LQC+N S   LP+ P
Sbjct: 243 SLMSPLPIVYSSTLKQMIKLMLRKKPEYRPTACELLRNPSLQPYLLQCQNLSPIYLPVFP 302

Query: 301 -----DSKNKTPRKSPSNKLNSGHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENM 360
                  K+K  R S   K      SR K S V   LEN  L     +    +SS+ +  
Sbjct: 303 IKPVNSPKDKARRNSLPGKFGKERVSREK-SEVSRSLEN--LYPFWTNTETGSSSSSQPA 362

Query: 361 SLTAISEDILETKMVDP-------TSFPDEISTSNAVDTDAQTSSASVTSTCNGGNSIRC 420
           S T  +ED LETK +DP       + F  + S  + +D D    S    +  N       
Sbjct: 363 SSTNGAEDKLETKRIDPSCDTLKISEFTSQKSDESLIDPDIAVYSTETPAEENALPK-ET 422

Query: 421 TNSLQKEPRLEDV-------PKTSCNEPGTKAE-ETSEGNPMESS-----ISSI--RSTE 480
            N   +E +L DV        + +C+ P    E ET E  P         ISS+   STE
Sbjct: 423 ENIFSEESQLRDVDVGVVSAQEVACSPPRAIEEAETQEALPKPKEQITVPISSVAHSSTE 482

Query: 481 KSGSSDDKRFNVEPRSCLNQERVARSTLTEETHADCMLSESEYP-SQRRADALESLLELC 540
            + + D    ++E     ++ ++ + T +E +     L++   P S+ RADALE LLE C
Sbjct: 483 VAAAKDHLSGSLEG----DKAKMVKLTASEMSSVLSKLTKLGPPQSKERADALECLLEKC 542

BLAST of Carg22477 vs. TAIR 10
Match: AT3G44200.1 (NIMA (never in mitosis, gene A)-related 6 )

HSP 1 Score: 431.0 bits (1107), Expect = 1.4e-120
Identity = 217/349 (62.18%), Postives = 272/349 (77.94%), Query Frame = 0

Query: 8   MKNRMEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIA 67
           M++RM+ YE++EQIGRGAFG+A LV+HK E+KKYVLKKIRLA+QTE+ +R+AHQEM+LIA
Sbjct: 1   MESRMDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIA 60

Query: 68  TLNHPYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKWLAQLLLA 127
            + HPYIV++K++WV+KG  +CIVTGYCEGGDM+EL+KK+ G YFPEEKLCKW  QLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLA 120

Query: 128 VDYLHSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNYMCPELLT 187
           V+YLHSN VLHRDLKCSNIFLTK  D+RLGDFGLAK L  +DL SSVVGTPNYMCPELL 
Sbjct: 121 VEYLHSNYVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLA 180

Query: 188 DIPYGYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSSALKQIIK 247
           DIPYG+KSDIWSLGCC++E++A++PAF+A DMAGLI+K+NRSSI PLP  YS +LK +IK
Sbjct: 181 DIPYGFKSDIWSLGCCIYEMAAYRPAFKAFDMAGLISKVNRSSIGPLPPCYSPSLKALIK 240

Query: 248 SMLRKNPGHRPTAAELLRHPHIQPYVLQCRNT--SANILPICPDSKNKTPRKSPSNKLNS 307
            MLRKNP +RP A+E+L+HP++QPYV Q R T  +A+I P  P   ++  R+S +   NS
Sbjct: 241 GMLRKNPEYRPNASEILKHPYLQPYVEQYRPTLSAASITPEKP-LNSREGRRSMAESQNS 300

Query: 308 GHESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILE 355
              S      V +  +NI  V  S    N N  T+ +       EDIL+
Sbjct: 301 NSSSEKDNFYVSD--KNIRYVVPS----NGNKVTETDSGFVD-DEDILD 341


HSP 2 Score: 45.8 bits (107), Expect = 1.3e-04
Identity = 87/326 (26.69%), Postives = 135/326 (41.41%), Query Frame = 0

Query: 238 YSSALKQIIKSMLRKNPGHRPTAAELLRHPHIQPYVLQCRNTSANILP-----ICPD-SK 297
           YS +   I   M+ +   H  +    +R   I   V   +NT A+  P      CP   K
Sbjct: 625 YSYSFPDISSEMMIRRDEHSTS----MRLTEIPDSVSGVQNTIAHHQPEREQGSCPTVLK 684

Query: 298 NKTPRKSPSNKLNSGHESRGKPSVVFNLLENIDLVAGSADI-------HNSNSSTDENMS 357
           + +P    S + N+     G     F + E +  V G A +       H  NS +D   S
Sbjct: 685 DDSPATLQSYEPNTSQHQHGDDK--FTVKEFVSSVPGPAPLPLHVEPSHQVNSHSDNKTS 744

Query: 358 LTAISEDILETKM-VDPTSFPDEI-----STSNAVDTDAQTSSASVTSTCNGG-----NS 417
           + + +  + +      P    D++      +S  V +D     +      N       N 
Sbjct: 745 VMSQNSALEKNNSHSHPHPVVDDVIHVIRHSSFRVGSDQPVMESVEVGVQNVDMGKLINV 804

Query: 418 IRCTNSLQKEPRLEDVPKTS--CNEPGTKAEETSEGNPMESSISSIRSTEKSGSSDDKRF 477
           +R    ++K     + P T    +EP ++ E   +     ++ S  +S   +  SD    
Sbjct: 805 VRDEMEVRKGATPSESPTTRSIISEPDSRTEPRPKEPDPITNYSETKSF--NSCSDSSPA 864

Query: 478 NVEPRSCLNQERVARSTLTEETHADCMLSESEYPSQRRADALESLLELCARLLKQDKLEE 537
                S + +E    +   +ET     L    +  ++RA+ALE LLEL A LL+Q +LEE
Sbjct: 865 ETRTNSFVPEEETTPTPPVKET-----LDIKSF--RQRAEALEGLLELSADLLEQSRLEE 924

BLAST of Carg22477 vs. TAIR 10
Match: AT3G63280.1 (NIMA-related kinase 4 )

HSP 1 Score: 419.5 bits (1077), Expect = 4.3e-117
Identity = 244/556 (43.88%), Postives = 349/556 (62.77%), Query Frame = 0

Query: 12  MEDYEVLEQIGRGAFGSAFLVYHKIEKKKYVLKKIRLAKQTEKFKRTAHQEMNLIATLNH 71
           ME YEVLEQIG+G+FGSA LV HK E+KKYVLKKIRLA+Q+++ +R+AHQEM LI+T+ +
Sbjct: 1   MERYEVLEQIGKGSFGSALLVRHKQERKKYVLKKIRLARQSDRARRSAHQEMELISTVRN 60

Query: 72  PYIVDYKDSWVDKGDCICIVTGYCEGGDMSELIKKAKGTYFPEEKLCKWLAQLLLAVDYL 131
           P++V+YKDSWV+KG  +CIV GYC+GGDM++ IK+A G +FPEEKLC+WL QLL+A+DYL
Sbjct: 61  PFVVEYKDSWVEKGCYVCIVIGYCQGGDMTDTIKRACGVHFPEEKLCQWLVQLLMALDYL 120

Query: 132 HSNRVLHRDLKCSNIFLTKGNDIRLGDFGLAKLLNTEDLASSVVGTPNYMCPELLTDIPY 191
           HSN +LHRD+KCSNIFLTK  DIRLGDFGLAK+L ++DL SSVVGTP+YMCPELL DIPY
Sbjct: 121 HSNHILHRDVKCSNIFLTKEQDIRLGDFGLAKILTSDDLTSSVVGTPSYMCPELLADIPY 180

Query: 192 GYKSDIWSLGCCMFEISAHQPAFRAPDMAGLINKINRSSISPLPIVYSSALKQIIKSMLR 251
           G KSDIWSLGCCM+E++AH+P F+A D+  LI KI++  + P+P +YS + + +IKSMLR
Sbjct: 181 GSKSDIWSLGCCMYEMAAHKPPFKASDVQTLITKIHKLIMDPIPAMYSGSFRGLIKSMLR 240

Query: 252 KNPGHRPTAAELLRHPHIQPYV----LQCRNTSANILPICPDSKNKTPRKSPSNKLNSGH 311
           KNP  RP+A ELL HPH+QPY+    ++  +   +  P+    ++ T ++    + +  +
Sbjct: 241 KNPELRPSANELLNHPHLQPYISMVYMKLESPRRSTFPLQFSERDATLKE--RRRSSFSN 300

Query: 312 ESRGKPSVVFNLLENIDLVAGSADIHNSNSSTDENMSLTAISEDILETKMVDPTSFPDEI 371
           + R  PSV      ++     ++     N      +++  + E+I+  +  +        
Sbjct: 301 DRRLNPSVSDTEAGSVSSSGKASPTPMFNGRKVSEVTVGVVREEIVPQRQEEAKKQSGAA 360

Query: 372 STSNAVDTDAQTSSASVTSTCNGGNSIRCTNSLQKEPRLEDVPKTSCNEPGTKAEETS-- 431
            T     T A+   AS   T      ++ +N  ++  R+  +P    N    +++ T+  
Sbjct: 361 RTPRVAGTSAK---ASTQRTVFKHELMKVSNPTERRRRV-SLPLVVENPYTYESDITALC 420

Query: 432 EGNPMESSISSIRSTEKSGSSDDKRFNVEPRSCLNQERVARSTLTEE------------- 491
             N  + S+++ R  +K     +  F  + R   ++  VAR + +               
Sbjct: 421 SLNSPDVSVNTPR-FDKIAEFPEDIFQNQNRETASRREVARHSFSSPPCPPHGEDNSNGS 480

Query: 492 -THADCML---SESEYPS--------QRRADALESLLELCARLLKQDKLEELAGVLSPFG 537
            T   C +   S SE           Q+RA+ALE LLE  A+LL+Q++ +EL  +L PFG
Sbjct: 481 ITKDKCTVQKRSVSEVKQRRFDTSSYQQRAEALEGLLEFSAKLLQQERYDELGVLLKPFG 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7033550.10.0e+00100.00Serine/threonine-protein kinase Nek6 [Cucurbita argyrosperma subsp. argyrosperma... [more]
KAG6603248.12.3e-30699.45Serine/threonine-protein kinase Nek6, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022928610.16.9e-30398.34serine/threonine-protein kinase Nek6-like [Cucurbita moschata] >XP_022928611.1 s... [more]
XP_023543552.12.1e-29194.74serine/threonine-protein kinase Nek6-like [Cucurbita pepo subsp. pepo] >XP_02354... [more]
XP_022967762.11.4e-28493.77serine/threonine-protein kinase Nek6-like [Cucurbita maxima] >XP_022967764.1 ser... [more]
Match NameE-valueIdentityDescription
Q9LT355.6e-13850.93Serine/threonine-protein kinase Nek6 OS=Arabidopsis thaliana OX=3702 GN=NEK6 PE=... [more]
Q6YY752.8e-13752.43Serine/threonine-protein kinase Nek6 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q9LHI71.6e-12448.42Serine/threonine-protein kinase Nek7 OS=Arabidopsis thaliana OX=3702 GN=NEK7 PE=... [more]
A2ZMH21.2e-12145.95Serine/threonine-protein kinase Nek2 OS=Oryza sativa subsp. indica OX=39946 GN=N... [more]
Q2QMH13.6e-12145.78Serine/threonine-protein kinase Nek2 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1EKS23.4e-30398.34serine/threonine-protein kinase Nek6-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1HRP87.0e-28593.77serine/threonine-protein kinase Nek6-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A1S3B6189.6e-23468.81serine/threonine-protein kinase Nek6 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3E2489.6e-23468.81Serine/threonine-protein kinase Nek6 isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
A0A5A7T2B99.6e-23468.81Serine/threonine-protein kinase Nek6 isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
Match NameE-valueIdentityDescription
AT3G20860.11.8e-13950.74NIMA-related kinase 5 [more]
AT3G12200.11.1e-12548.42NIMA-related kinase 7 [more]
AT3G12200.23.1e-12347.59NIMA-related kinase 7 [more]
AT3G44200.11.4e-12062.18NIMA (never in mitosis, gene A)-related 6 [more]
AT3G63280.14.3e-11743.88NIMA-related kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 15..269
e-value: 3.4E-89
score: 312.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 15..268
e-value: 1.0E-65
score: 221.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 15..269
score: 47.085312
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 2..94
e-value: 6.8E-24
score: 86.0
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 95..283
e-value: 8.3E-58
score: 197.0
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 12..270
e-value: 2.0E-28
score: 97.0
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 18..212
e-value: 1.4E-11
score: 42.0
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 13..265
e-value: 6.5E-22
score: 75.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 417..441
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 360..452
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 287..311
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 362..400
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 287..308
NoneNo IPR availablePANTHERPTHR43671:SF70SERINE/THREONINE-PROTEIN KINASE NEK7coord: 460..533
coord: 8..416
NoneNo IPR availablePANTHERPTHR43671SERINE/THREONINE-PROTEIN KINASE NEKcoord: 460..533
coord: 8..416
NoneNo IPR availableCDDcd08215STKc_Nekcoord: 14..269
e-value: 8.57789E-136
score: 392.596
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 136..148
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 21..44
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 12..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22477-RACarg22477-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity