Carg22432 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22432
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein WHAT'S THIS FACTOR 1
LocationCarg_Chr14: 11184758 .. 11186215 (-)
RNA-Seq ExpressionCarg22432
SyntenyCarg22432
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTATCTTCTCAGTTCTTGACCCCTAGGGACTGGCATGTAACATCCCTTAGTTCAAACCTTCTTTGTGGTACTCCATTGTGGTTGCACTCCAATGTCGGTTGCAAGTACCAAAGAAAAGAAAATTTCAAGACTTGTTATACTTTAACACCTCGTGCCTCTGTTAAGATTGTCCGTAGTCGTCCATTAGATCGGCATGCTGTAAAACATAACAAAACTCGATTTGTACAAAAGCTGATAATCCTGCTGCTGTCTAAACCAAAGCATTATATACCAATCCACATTCTTTCCAAGTGTCGTGGCTATCTTTCCCTTCCCAAACCCCGCTCTCTTCTTTCAATGATTCATCGTTATCCTTCCATTTTTGAACTTTTTTCAATCCCTTATCCACCCACACCACTCAATGCAACTAAGCTATACCCCCAACTTTGTGTTCGTCTAACCCCAGTAGCAGCATCTCTTGCTAAGCAGGACTCCAATCTCAAATTGGTGATCTCTAACACCCTGGCTGAAAAGCTCCAGAAGTTACTTATGCTTTCTTCGCACCACAGGATTCTCTTATCCAAGCTGGTTCACCTTGCTCCCGACCTAAGCATGCCCCCAAATTTTAGGTCCCGTCTCTGTAATGATTATCCAGAAAAATTCAGGACTGTCGACACTTCATATGGCCGTGCACTGGAGCTTGTCTTCTGGGACCCAGAGTTGGCAAAGCCTTTACCTTGCCTTCAAGTTTCTTCACGTGAGCTAATAGTGGATAGACCTTTAAAATTCAACTTGTTGAAACTAAGAAAGGGACTGAACTTGAAAAGAGCCCACCAGGAATTTCTAATTAAGTTCAGGGATTTGCCAGATGTTTGCCCTTACAAAACTCCTGCGAGTGAGTTGGCTAAGGAGTCTCTCGAGTCAGAGAAACGAGCTTGTGCTGTAGTGCGAGAGGTATTGGGGATGATGATTGAGAAGAGGACTTTAATAGATCACTTGACACATTTTAGAAAAGATTTTGGGCTACCCAATAAACTTAGAGGAATGATTGTGAGGCATCCAGAGTTATTTTATGTGAGTCTGAAGGGTCAGAGAGACTCTGTATTCCTTGTAGAGGGGTTCAACGATAAGGGTCATCTAATAGAGAAGGATGAGACTTTGGCTATCAAAAATCAATGGATGAAGCTTTTGATGGAAGGGAAAAGGATGAGACGGGAGAAGAGGAAGGCTCAAATATATGACAGTAGATATGGAAATGATCATGAAAATCATAGTCATGACCATGAGATGGAAACTGACTATGATGATGATTACGAAGATGGTTTTGAGAGTTTATTTCAGTATGAGGATTTAGATTTTGAGGATGAGGAGAGTGATCTGCCAAGCAATAGGTCAAATGGAGACTTTTGGACTACAAATAATGCAGCAGATATTATAAATGGCGAAAATGGAGGACATATAAAACCGTGGTGA

mRNA sequence

ATGGCTTTATCTTCTCAGTTCTTGACCCCTAGGGACTGGCATGTAACATCCCTTAGTTCAAACCTTCTTTGTGGTACTCCATTGTGGTTGCACTCCAATGTCGGTTGCAAGTACCAAAGAAAAGAAAATTTCAAGACTTGTTATACTTTAACACCTCGTGCCTCTGTTAAGATTGTCCGTAGTCGTCCATTAGATCGGCATGCTGTAAAACATAACAAAACTCGATTTGTACAAAAGCTGATAATCCTGCTGCTGTCTAAACCAAAGCATTATATACCAATCCACATTCTTTCCAAGTGTCGTGGCTATCTTTCCCTTCCCAAACCCCGCTCTCTTCTTTCAATGATTCATCGTTATCCTTCCATTTTTGAACTTTTTTCAATCCCTTATCCACCCACACCACTCAATGCAACTAAGCTATACCCCCAACTTTGTGTTCGTCTAACCCCAGTAGCAGCATCTCTTGCTAAGCAGGACTCCAATCTCAAATTGGTGATCTCTAACACCCTGGCTGAAAAGCTCCAGAAGTTACTTATGCTTTCTTCGCACCACAGGATTCTCTTATCCAAGCTGGTTCACCTTGCTCCCGACCTAAGCATGCCCCCAAATTTTAGGTCCCGTCTCTGTAATGATTATCCAGAAAAATTCAGGACTGTCGACACTTCATATGGCCGTGCACTGGAGCTTGTCTTCTGGGACCCAGAGTTGGCAAAGCCTTTACCTTGCCTTCAAGTTTCTTCACGTGAGCTAATAGTGGATAGACCTTTAAAATTCAACTTGTTGAAACTAAGAAAGGGACTGAACTTGAAAAGAGCCCACCAGGAATTTCTAATTAAGTTCAGGGATTTGCCAGATGTTTGCCCTTACAAAACTCCTGCGAGTGAGTTGGCTAAGGAGTCTCTCGAGTCAGAGAAACGAGCTTGTGCTGTAGTGCGAGAGGTATTGGGGATGATGATTGAGAAGAGGACTTTAATAGATCACTTGACACATTTTAGAAAAGATTTTGGGCTACCCAATAAACTTAGAGGAATGATTGTGAGGCATCCAGAGTTATTTTATGTGAGTCTGAAGGGTCAGAGAGACTCTGTATTCCTTGTAGAGGGGTTCAACGATAAGGGTCATCTAATAGAGAAGGATGAGACTTTGGCTATCAAAAATCAATGGATGAAGCTTTTGATGGAAGGGAAAAGGATGAGACGGGAGAAGAGGAAGGCTCAAATATATGACAGTAGATATGGAAATGATCATGAAAATCATAGTCATGACCATGAGATGGAAACTGACTATGATGATGATTACGAAGATGGTTTTGAGAGTTTATTTCAGTATGAGGATTTAGATTTTGAGGATGAGGAGAGTGATCTGCCAAGCAATAGGTCAAATGGAGACTTTTGGACTACAAATAATGCAGCAGATATTATAAATGGCGAAAATGGAGGACATATAAAACCGTGGTGA

Coding sequence (CDS)

ATGGCTTTATCTTCTCAGTTCTTGACCCCTAGGGACTGGCATGTAACATCCCTTAGTTCAAACCTTCTTTGTGGTACTCCATTGTGGTTGCACTCCAATGTCGGTTGCAAGTACCAAAGAAAAGAAAATTTCAAGACTTGTTATACTTTAACACCTCGTGCCTCTGTTAAGATTGTCCGTAGTCGTCCATTAGATCGGCATGCTGTAAAACATAACAAAACTCGATTTGTACAAAAGCTGATAATCCTGCTGCTGTCTAAACCAAAGCATTATATACCAATCCACATTCTTTCCAAGTGTCGTGGCTATCTTTCCCTTCCCAAACCCCGCTCTCTTCTTTCAATGATTCATCGTTATCCTTCCATTTTTGAACTTTTTTCAATCCCTTATCCACCCACACCACTCAATGCAACTAAGCTATACCCCCAACTTTGTGTTCGTCTAACCCCAGTAGCAGCATCTCTTGCTAAGCAGGACTCCAATCTCAAATTGGTGATCTCTAACACCCTGGCTGAAAAGCTCCAGAAGTTACTTATGCTTTCTTCGCACCACAGGATTCTCTTATCCAAGCTGGTTCACCTTGCTCCCGACCTAAGCATGCCCCCAAATTTTAGGTCCCGTCTCTGTAATGATTATCCAGAAAAATTCAGGACTGTCGACACTTCATATGGCCGTGCACTGGAGCTTGTCTTCTGGGACCCAGAGTTGGCAAAGCCTTTACCTTGCCTTCAAGTTTCTTCACGTGAGCTAATAGTGGATAGACCTTTAAAATTCAACTTGTTGAAACTAAGAAAGGGACTGAACTTGAAAAGAGCCCACCAGGAATTTCTAATTAAGTTCAGGGATTTGCCAGATGTTTGCCCTTACAAAACTCCTGCGAGTGAGTTGGCTAAGGAGTCTCTCGAGTCAGAGAAACGAGCTTGTGCTGTAGTGCGAGAGGTATTGGGGATGATGATTGAGAAGAGGACTTTAATAGATCACTTGACACATTTTAGAAAAGATTTTGGGCTACCCAATAAACTTAGAGGAATGATTGTGAGGCATCCAGAGTTATTTTATGTGAGTCTGAAGGGTCAGAGAGACTCTGTATTCCTTGTAGAGGGGTTCAACGATAAGGGTCATCTAATAGAGAAGGATGAGACTTTGGCTATCAAAAATCAATGGATGAAGCTTTTGATGGAAGGGAAAAGGATGAGACGGGAGAAGAGGAAGGCTCAAATATATGACAGTAGATATGGAAATGATCATGAAAATCATAGTCATGACCATGAGATGGAAACTGACTATGATGATGATTACGAAGATGGTTTTGAGAGTTTATTTCAGTATGAGGATTTAGATTTTGAGGATGAGGAGAGTGATCTGCCAAGCAATAGGTCAAATGGAGACTTTTGGACTACAAATAATGCAGCAGATATTATAAATGGCGAAAATGGAGGACATATAAAACCGTGGTGA

Protein sequence

MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVRSRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPLPCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHSHDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGGHIKPW
Homology
BLAST of Carg22432 vs. NCBI nr
Match: XP_022955645.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita moschata] >XP_022955646.1 protein WHAT'S THIS FACTOR 1 [Cucurbita moschata] >KAG7018490.1 Protein ROOT PRIMORDIUM DEFECTIVE 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 975.7 bits (2521), Expect = 1.5e-280
Identity = 485/485 (100.00%), Postives = 485/485 (100.00%), Query Frame = 0

Query: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60
           MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR
Sbjct: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60

Query: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120
           SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP
Sbjct: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240

Query: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300
           PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES
Sbjct: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360
           LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420
           DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS
Sbjct: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420

Query: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480
           HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG
Sbjct: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480

Query: 481 HIKPW 486
           HIKPW
Sbjct: 481 HIKPW 485

BLAST of Carg22432 vs. NCBI nr
Match: KAG6582056.1 (Protein WHAT'S THIS FACTOR 1-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 969.9 bits (2506), Expect = 8.0e-279
Identity = 483/484 (99.79%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60
           MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR
Sbjct: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60

Query: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120
           SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP
Sbjct: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240

Query: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300
           PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES
Sbjct: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360
           LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420
           DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS
Sbjct: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420

Query: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480
           HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG
Sbjct: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480

Query: 481 HIKP 485
           HI+P
Sbjct: 481 HIEP 484

BLAST of Carg22432 vs. NCBI nr
Match: XP_023527715.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 953.4 bits (2463), Expect = 7.7e-274
Identity = 476/485 (98.14%), Postives = 479/485 (98.76%), Query Frame = 0

Query: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60
           MALSSQFLTPRDW+VT LSSNLL GT LWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR
Sbjct: 1   MALSSQFLTPRDWYVTPLSSNLLRGTTLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60

Query: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120
           SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP
Sbjct: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTP AASLAKQDSNLKLVISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLAKQDSNLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240

Query: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300
           PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES
Sbjct: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360
           LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420
           DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHEN+S
Sbjct: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENYS 420

Query: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480
           HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIIN  NGG
Sbjct: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINDANGG 480

Query: 481 HIKPW 486
           HI+PW
Sbjct: 481 HIEPW 485

BLAST of Carg22432 vs. NCBI nr
Match: XP_022979770.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita maxima] >XP_022979771.1 protein WHAT'S THIS FACTOR 1 [Cucurbita maxima])

HSP 1 Score: 952.2 bits (2460), Expect = 1.7e-273
Identity = 476/485 (98.14%), Postives = 478/485 (98.56%), Query Frame = 0

Query: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60
           MALSSQFLTPRDWHVTS SSNLL GTPLWLHSNVGCKYQRKENF+T YTLTPRASVKIVR
Sbjct: 1   MALSSQFLTPRDWHVTSRSSNLLRGTPLWLHSNVGCKYQRKENFETSYTLTPRASVKIVR 60

Query: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120
           S PLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP
Sbjct: 61  SCPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTP AASLAKQDSNLKLVISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLAKQDSNLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAK L
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKTL 240

Query: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300
           PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES
Sbjct: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360
           LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420
           DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS
Sbjct: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420

Query: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480
           HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADI NGENGG
Sbjct: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADITNGENGG 480

Query: 481 HIKPW 486
           HI+PW
Sbjct: 481 HIEPW 485

BLAST of Carg22432 vs. NCBI nr
Match: XP_038904265.1 (protein WHAT'S THIS FACTOR 1, chloroplastic [Benincasa hispida] >XP_038904305.1 protein WHAT'S THIS FACTOR 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 879.0 bits (2270), Expect = 1.9e-251
Identity = 438/485 (90.31%), Postives = 461/485 (95.05%), Query Frame = 0

Query: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60
           M LSS FLTP DWHVT LSSNLLCG PLWLHSNV CKYQRKENF+T YTLTP +S+KIVR
Sbjct: 1   MVLSSHFLTPTDWHVTLLSSNLLCGNPLWLHSNVSCKYQRKENFETRYTLTPCSSLKIVR 60

Query: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120
           SR LDRHAVKHNKTRFVQKLIILLLSKPKHYIP+HILSKCRGYLSLP+PRSLLSMIHRYP
Sbjct: 61  SRSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLSLPRPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATK+YPQLCVRLTP AASLAKQDS+LKLVISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKVYPQLCVRLTPAAASLAKQDSDLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELV WDPELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDPELAKPL 240

Query: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300
           PCLQV SRELIVDRPLKFNLL+LRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES
Sbjct: 241 PCLQVPSRELIVDRPLKFNLLRLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360
           LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420
           DSVFLVEGF++KG L++KDETLAIKNQWMKLLMEGKRMR+EK+KA+ YDS+YGN+H+N +
Sbjct: 361 DSVFLVEGFDEKGVLVKKDETLAIKNQWMKLLMEGKRMRQEKKKARKYDSKYGNEHKNDN 420

Query: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480
           HDHEMETD+DDDY+DGFESLFQYEDLDFEDE SDLPSN  NGDFW TNN  DIIN  +GG
Sbjct: 421 HDHEMETDFDDDYDDGFESLFQYEDLDFEDERSDLPSNWLNGDFWITNN-VDIINDADGG 480

Query: 481 HIKPW 486
            I+PW
Sbjct: 481 RIEPW 484

BLAST of Carg22432 vs. ExPASy Swiss-Prot
Match: B6TTV8 (Protein WHAT'S THIS FACTOR 1, chloroplastic OS=Zea mays OX=4577 GN=WTF1 PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.4e-60
Identity = 155/420 (36.90%), Postives = 226/420 (53.81%), Query Frame = 0

Query: 53  RASVKIVRSRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSL 112
           +A+VK  +  P D    +  K + V KL  +L+++P   + +  L + R  L L + R L
Sbjct: 52  QAAVKRRKEAPFDTVIQRDKKLKLVLKLRNILVAQPDRVMSLRELGRFRRDLGLTRKRRL 111

Query: 113 LSMIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAE 172
           ++++ R+P +F++              +Y  L  RLTP A  L   +  L+         
Sbjct: 112 IALLRRFPGVFDVVE----------EGVY-SLRFRLTPAAERLYLDELRLRNESEGLAVA 171

Query: 173 KLQKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFW 232
           KL+KLLM+S   RIL+ K+ HL  DL +PP FR  +C  YP+ FR V    G ALEL  W
Sbjct: 172 KLRKLLMMSQEKRILIEKVAHLKHDLGLPPEFRDTVCLRYPQYFRVVRMDRGPALELTHW 231

Query: 233 DPELAKPLPCL--------QVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLP 292
           DPELA     L        +   R LI+DRPLKFN ++L KGL L R     + +F+++P
Sbjct: 232 DPELAVSAAELAEEESRAREAEERNLIIDRPLKFNRVRLPKGLKLTRGEARRIARFKEMP 291

Query: 293 DVCPYKTPASELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGM 352
            + PY    S L   S E EK AC VV E+L + +EKRTL+DHLTHFR++F     LRGM
Sbjct: 292 YISPY-ADFSHLRSGSDEKEKHACGVVHEILSLTVEKRTLVDHLTHFREEFRFSQSLRGM 351

Query: 353 IVRHPELFYVSLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRK 412
           I+RHP++FYVS KG RDSVFL E + D   L+EK++ + +K + M+ L+   R  R +  
Sbjct: 352 IIRHPDMFYVSFKGDRDSVFLREAYKD-SQLVEKNQLVLLKEK-MRALVAVPRFPR-RAA 411

Query: 413 AQIYDSRYGNDHENHSHDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDF 465
               +   G +    S D   + +YDD+ ++G          D ED  S+L   +S+ D+
Sbjct: 412 VGTGEEAEGMNGSLQSRDQVSDEEYDDE-DEGLS--------DMEDLISELSGGKSDADY 447

BLAST of Carg22432 vs. ExPASy Swiss-Prot
Match: Q65XL5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0571100 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 2.7e-59
Identity = 158/431 (36.66%), Postives = 228/431 (52.90%), Query Frame = 0

Query: 53  RASVKIVRSRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSL 112
           +A+VK  +  P D    +  K + V KL  +L+S P   + +  L + R  L L + R L
Sbjct: 58  QAAVKRRKEIPFDNVIQRDKKLKLVLKLRNILVSNPDRVMSLRDLGRFRRDLGLTRKRRL 117

Query: 113 LSMIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAE 172
           ++++ R+P +FE+              +Y  L  RLTP A  L   + +LK         
Sbjct: 118 IALLKRFPGVFEVVE----------EGVY-SLRFRLTPAAERLYLDELHLKNESEGLAVT 177

Query: 173 KLQKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFW 232
           KL+KLLM+S   RIL+ K+ HL  DL +PP FR  +C  YP+ FR V    G  LEL  W
Sbjct: 178 KLRKLLMMSQDKRILIEKIAHLKNDLGLPPEFRDTICLRYPQYFRVVQMDRGPGLELTHW 237

Query: 233 DPELA--------KPLPCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLP 292
           DPELA        +     +   R LI+DRPLKFN +KL +GL L R     + +F+++P
Sbjct: 238 DPELAVSAAEVAEEENRAREEQERNLIIDRPLKFNRVKLPQGLKLSRGEARRVAQFKEMP 297

Query: 293 DVCPYKTPASELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGM 352
            + PY +  S L   S E EK AC VV E+L + +EKRTL+DHLTHFR++F     LRGM
Sbjct: 298 YISPY-SDFSHLRSGSAEKEKHACGVVHEILSLTLEKRTLVDHLTHFREEFRFSQSLRGM 357

Query: 353 IVRHPELFYVSLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRK 412
           ++RHP++FYVSLKG RDSVFL E + +   L+EK + + +K + M+ L+   R  R    
Sbjct: 358 LIRHPDMFYVSLKGDRDSVFLREAYKN-SQLVEKSKLVLLKEK-MRALVAVPRFPRRGVP 417

Query: 413 AQIYDSRYGNDHENHSHDHEME-TDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSN-- 470
           A   ++    D  N +     E +D +DD ++G          D ED  S++   +S+  
Sbjct: 418 ATSEEA----DRTNGAAQMLSEGSDVEDDEDEGLS--------DMEDLISEISGGKSDTD 462

BLAST of Carg22432 vs. ExPASy Swiss-Prot
Match: A0MFS5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01037 PE=3 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 5.9e-59
Identity = 161/439 (36.67%), Postives = 232/439 (52.85%), Query Frame = 0

Query: 45  KTCYTLTP-RASVKIVRSRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGY 104
           KT   + P RA+VK  +    D    +  K + V  +  +L+S+P   + +  L K R  
Sbjct: 64  KTRVVVEPVRAAVKRRKELTFDSVVQRDKKLKLVLNIRKILVSQPDRMMSLRGLGKYRRD 123

Query: 105 LSLPKPRSLLSMIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLK 164
           L L K R  ++++ +YP +FE+             +    L  ++T  A  L   +  ++
Sbjct: 124 LGLKKRRRFIALLRKYPGVFEI-----------VEEGAYSLRFKMTSEAERLYLDEMRIR 183

Query: 165 LVISNTLAEKLQKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSY 224
             + + L  KL+KL+M+S   RILL K+ HL  DL +P  FR  +C  YP+ FR V T  
Sbjct: 184 NELEDVLVVKLRKLVMMSIDKRILLEKISHLKTDLGLPLEFRDTICQRYPQYFRVVPTPR 243

Query: 225 GRALELVFWDPELAKPLPCL--------QVSSRELIVDRPLKFNLLKLRKGLNLKRAHQE 284
           G ALEL  WDPELA     L        +   R LI+DRP KFN +KL +GLNL ++   
Sbjct: 244 GPALELTHWDPELAVSAAELSEDDNRTRESEERNLIIDRPPKFNRVKLPRGLNLSKSETR 303

Query: 285 FLIKFRDLPDVCPYKTPASELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDF 344
            + +FRD+  + PYK   S L   +LE EK AC V+ E+L +  EKRTL+DHLTHFR++F
Sbjct: 304 KISQFRDMQYISPYK-DFSHLRSGTLEKEKHACGVIHELLSLTTEKRTLVDHLTHFREEF 363

Query: 345 GLPNKLRGMIVRHPELFYVSLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLL--- 404
               +LRGM++RHP+LFYVSLKG+RDSVFL E + +   LI+KD    +K +   L+   
Sbjct: 364 RFSQQLRGMLIRHPDLFYVSLKGERDSVFLREAYRN-SELIDKDPLTLVKEKMRALVSVP 423

Query: 405 ----MEGKRMRREKRKAQIYDSRYGNDHENHSHDHEMETDYDD--DYEDGFESLFQYEDL 464
                 G R   E R+ +I  S    + E    D E  +D D   + EDG       +D 
Sbjct: 424 RFPRRGGPRKDEEGREVEIDGSDADGEEEEEESDAEEWSDVDGYLEGEDGGND----DDG 483

BLAST of Carg22432 vs. ExPASy Swiss-Prot
Match: Q689D6 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=RPD1 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.6e-19
Identity = 97/377 (25.73%), Postives = 176/377 (46.68%), Query Frame = 0

Query: 59  VRSRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLP-KPRSLLSMIH 118
           VR    D +     K R V K   L+LS+P H I I +L      L L  K     + + 
Sbjct: 47  VRDHGYDNYMEVEKKIRKVVKFHSLILSQPNHTIAISLLDTLARRLGLGFKQHEPGAFLL 106

Query: 119 RYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKL 178
           ++P +FE++  P          +   L  RLT  A    + +    L        +L+KL
Sbjct: 107 KFPHVFEIYEHP----------VQRILYCRLTRKALDQIRHEHEAVLDQIPDAVTRLRKL 166

Query: 179 LMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVD--TSYGRALELVFWDPE 238
           +M+S+  RI L  +     +  +P +F   +   +P+ FR +D   +  + +E+V  DP 
Sbjct: 167 VMMSNTGRIRLEHVRIARTEFGLPEDFEYSVILKHPQFFRLIDGEETRDKYIEIVEKDPN 226

Query: 239 LAKPLPCLQVSSREL------IVDRPLKFN-LLKLRKGLNLKRAHQEFLIKFRDLPDVCP 298
           L+    C     RE+      I    ++F+ ++    G  + +  +  + K++ LP   P
Sbjct: 227 LS---ICAIERVREIEYRTKGIDAEDVRFSFVVNFPPGFKIGKYFRIAVWKWQRLPYWSP 286

Query: 299 YKTPASELAKESLES----EKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGM 358
           Y+   S     S+E+    EKR+ A + E+L + +EK+  ++ + HFR    LP KL+  
Sbjct: 287 YE-DISGYDLRSMEAQNRLEKRSVACIHELLSLTVEKKITLERIAHFRNVMNLPKKLKEF 346

Query: 359 IVRHPELFYVSLK---GQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRRE 418
           +++H  +FY+S +   G+  +VFL EG+  +G L+E ++    + +  +L++     R+ 
Sbjct: 347 LLQHQGIFYISTRGNYGKLHTVFLREGYK-RGELVEPNDVYLARRRLAELVLMSP--RKA 406

BLAST of Carg22432 vs. ExPASy Swiss-Prot
Match: Q9ZUZ6 (Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=WTF9 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 5.1e-18
Identity = 85/347 (24.50%), Postives = 160/347 (46.11%), Query Frame = 0

Query: 67  HAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYPSIFELF 126
           H ++ ++ + V  L   ++ +P   IPI  +SK      +     +   + ++PSIFE F
Sbjct: 42  HILRSSQLKSVVSLKNCIVQEPNRCIPISAISKKTRQFDV--STKIAHFLRKFPSIFEEF 101

Query: 127 SIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLMLSSHHRI 186
             P    P            RLTP A  L +Q+  +    ++ L ++L+KL+++S  + +
Sbjct: 102 VGPEYNLP----------WFRLTPEATELDRQERVVYQTSADDLRDRLKKLILMSKDNVL 161

Query: 187 LLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTS---YGRALELVFWDPELAKPLPCL 246
            LS +  +   L +P ++      +    FR VD      G A++    D  L+      
Sbjct: 162 PLSIVQGMKWYLGLPDDYLQFPDMNLDSSFRFVDMEDGVKGLAVDYNGGDKVLSVLQKNA 221

Query: 247 QVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYK-----TPASELAK 306
               R  +    ++F L    KG  L+   +++L++F+ LP V PY       P+S++A 
Sbjct: 222 MKKRRGEVSLEEIEFPLFP-SKGCRLRVKIEDWLMEFQKLPYVSPYDDYSCLDPSSDIA- 281

Query: 307 ESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKG 366
                EKR    + E+L + +E       L   +K FGLP K+     RHP++FY+S+K 
Sbjct: 282 -----EKRVVGFLHELLCLFVEHSAERKKLLCLKKHFGLPQKVHKAFERHPQIFYLSMKN 341

Query: 367 QRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKA 406
           +  +  L E + DK   +E    L ++ ++++L+   + + + +R +
Sbjct: 342 KTCTAILREPYRDKAS-VETHPVLGVRKKYIQLMKNSELILKSRRNS 368

BLAST of Carg22432 vs. ExPASy TrEMBL
Match: A0A6J1GVP1 (protein WHAT'S THIS FACTOR 1 OS=Cucurbita moschata OX=3662 GN=LOC111457587 PE=4 SV=1)

HSP 1 Score: 975.7 bits (2521), Expect = 7.1e-281
Identity = 485/485 (100.00%), Postives = 485/485 (100.00%), Query Frame = 0

Query: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60
           MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR
Sbjct: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60

Query: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120
           SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP
Sbjct: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240

Query: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300
           PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES
Sbjct: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360
           LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420
           DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS
Sbjct: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420

Query: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480
           HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG
Sbjct: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480

Query: 481 HIKPW 486
           HIKPW
Sbjct: 481 HIKPW 485

BLAST of Carg22432 vs. ExPASy TrEMBL
Match: A0A6J1IUA5 (protein WHAT'S THIS FACTOR 1 OS=Cucurbita maxima OX=3661 GN=LOC111479376 PE=4 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 8.4e-274
Identity = 476/485 (98.14%), Postives = 478/485 (98.56%), Query Frame = 0

Query: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60
           MALSSQFLTPRDWHVTS SSNLL GTPLWLHSNVGCKYQRKENF+T YTLTPRASVKIVR
Sbjct: 1   MALSSQFLTPRDWHVTSRSSNLLRGTPLWLHSNVGCKYQRKENFETSYTLTPRASVKIVR 60

Query: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120
           S PLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP
Sbjct: 61  SCPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTP AASLAKQDSNLKLVISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLAKQDSNLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAK L
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKTL 240

Query: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300
           PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES
Sbjct: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360
           LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420
           DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS
Sbjct: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420

Query: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480
           HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADI NGENGG
Sbjct: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADITNGENGG 480

Query: 481 HIKPW 486
           HI+PW
Sbjct: 481 HIEPW 485

BLAST of Carg22432 vs. ExPASy TrEMBL
Match: A0A0A0KU86 (PORR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015830 PE=4 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.5e-246
Identity = 430/485 (88.66%), Postives = 454/485 (93.61%), Query Frame = 0

Query: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60
           MAL S FLTP DWHVTSLSSNLL G+PLWLHS V  K QRKENF+T YTLTP +S+KIVR
Sbjct: 1   MALCSHFLTPTDWHVTSLSSNLLSGSPLWLHSKVDFKCQRKENFRTHYTLTPCSSIKIVR 60

Query: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120
           SR LDRHAVKHNKTRFVQKLIILLLSKPKHYIP+HILSKCRGYLSLP+PRSLLSMIHRYP
Sbjct: 61  SRSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLSLPRPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTP AAS+AKQDS+LK+VISN LAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASIAKQDSDLKMVISNKLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240
           SSHHRILLSKLVHLAPDLS+PPNFRSRLCNDYPEKFRTVDTSYGRALELV WDPELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSLPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDPELAKPL 240

Query: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300
           PC+QV SRELIVDRPLKFNLL+LRKGLNLKR HQEFLIKFRDLPDVCPYK PASELAKES
Sbjct: 241 PCIQVPSRELIVDRPLKFNLLRLRKGLNLKRTHQEFLIKFRDLPDVCPYKNPASELAKES 300

Query: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360
           LESEKRACAVVREVLGMM+EKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMVEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420
           DSVFLVEGF+DKG L+EKDETLAIKNQWM LL E KR+RREK+KAQIYDS+YGNDHEN++
Sbjct: 361 DSVFLVEGFDDKGVLLEKDETLAIKNQWMTLLKEAKRLRREKKKAQIYDSKYGNDHENNN 420

Query: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480
           HDHEME DYDDDY+DGFESLFQYEDLDFEDE S +PS  SNGDFWTTNN  DI N  +GG
Sbjct: 421 HDHEMENDYDDDYDDGFESLFQYEDLDFEDENSGVPSIWSNGDFWTTNN-VDISNDADGG 480

Query: 481 HIKPW 486
           HI+PW
Sbjct: 481 HIEPW 484

BLAST of Carg22432 vs. ExPASy TrEMBL
Match: A0A5A7TP08 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003590 PE=4 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.8e-244
Identity = 431/485 (88.87%), Postives = 454/485 (93.61%), Query Frame = 0

Query: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60
           MALSS+FLTP DWH  SLSSNLL G+PLWLHS V  K QRKENF T YTLTP +S+KIVR
Sbjct: 1   MALSSRFLTPTDWH-ASLSSNLLSGSPLWLHSKVDFKCQRKENFITHYTLTPCSSIKIVR 60

Query: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120
           SR LDRHAVKHNKTRFVQKLIILLLSKPKHYIP+HILSKCRGYLSLP+PRSLLSMIHRYP
Sbjct: 61  SRSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLSLPRPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLT  AAS+AKQDS+LKLVISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTAAAASIAKQDSDLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELV WDPELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDPELAKPL 240

Query: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300
           PC+QV SRELIVDRPLKFNLL+LRKGLNLKR HQEFLIKFRDLPD+CPYKTPASELAKES
Sbjct: 241 PCIQVPSRELIVDRPLKFNLLRLRKGLNLKRTHQEFLIKFRDLPDICPYKTPASELAKES 300

Query: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360
           LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420
           DSVFLVEGF+DKG L+EKDETLAIKN+WM LL EGKRMRREK+KAQIYDS+YGNDHEN++
Sbjct: 361 DSVFLVEGFDDKGALLEKDETLAIKNRWMALLKEGKRMRREKKKAQIYDSKYGNDHENNN 420

Query: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480
           HDHEME DYDD+Y+DGFESLFQYEDLDFEDE S +PS  SNGDFWTTNN  DI N  +G 
Sbjct: 421 HDHEMENDYDDNYDDGFESLFQYEDLDFEDENSGVPSIWSNGDFWTTNN-VDISNDTDGS 480

Query: 481 HIKPW 486
           HI+PW
Sbjct: 481 HIEPW 483

BLAST of Carg22432 vs. ExPASy TrEMBL
Match: A0A1S3BYB2 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo OX=3656 GN=LOC103494529 PE=4 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 1.2e-243
Identity = 431/485 (88.87%), Postives = 452/485 (93.20%), Query Frame = 0

Query: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60
           MALSS FLTP DWH  SLSSNLL G+PLWLHS V  K QRKENF T YTLTP +S+KIVR
Sbjct: 1   MALSSHFLTPADWH-ASLSSNLLSGSPLWLHSKVDLKCQRKENFITHYTLTPCSSIKIVR 60

Query: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120
           SR LDRHAVKHNKTRFVQKLIILLLSKPKHYIP+HILSKCRGYLSLP+PRSLLSMIHRYP
Sbjct: 61  SRSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLSLPRPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLT  AAS+AKQDS+LKLVISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTAAAASIAKQDSDLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELV WDPELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDPELAKPL 240

Query: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300
           PC+QV SRELIVDRPLKFNLL+LRKGLNLKR HQEFLIKFRDLPDVCPYKTPASELAKES
Sbjct: 241 PCIQVPSRELIVDRPLKFNLLRLRKGLNLKRTHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360
           LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420
           DSVFLVEGF+DKG L+EKDETLAIKN+WM LL EGKRMRREK+KAQIYDS+YGNDHEN++
Sbjct: 361 DSVFLVEGFDDKGALLEKDETLAIKNRWMALLKEGKRMRREKKKAQIYDSKYGNDHENNN 420

Query: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480
           HDHEME DYDD+Y+DGFESLFQYEDLDFEDE S +PS  SNGDFWTTNN  DI N  +  
Sbjct: 421 HDHEMENDYDDNYDDGFESLFQYEDLDFEDENSGVPSIWSNGDFWTTNN-VDISNDTDES 480

Query: 481 HIKPW 486
           HI+PW
Sbjct: 481 HIEPW 483

BLAST of Carg22432 vs. TAIR 10
Match: AT5G62990.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.3e-141
Identity = 277/457 (60.61%), Postives = 332/457 (72.65%), Query Frame = 0

Query: 19  SSNLLCGTPLWL-HSNVGCKYQRKENFKTCYTLTPRASVKIVRSRPLDRHAVKHNKTRFV 78
           SS  L   PL +  SNV  K   K+       ++  A  KIVRS  LDRH VK N+ RFV
Sbjct: 20  SSFFLPSIPLEIRRSNVTTKTSSKKLGSLSLNISCSAH-KIVRSPSLDRHVVKQNRVRFV 79

Query: 79  QKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYPSIFELFSIPYPPTPLNA 138
           QKL  LLLSKPKHYIPI IL KCR YL +  P ++LSMI RYP+IFELF+ P P  P+NA
Sbjct: 80  QKLNTLLLSKPKHYIPIEILYKCRSYLCIENPLAILSMIRRYPTIFELFTTPTPHLPMNA 139

Query: 139 TKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLMLSSHHRILLSKLVHLAPD 198
           TK   QLCVRLT  A+SLA Q+ NLK  IS+ LA KLQKLLMLSSH R+LLSKLVH+APD
Sbjct: 140 TKPLSQLCVRLTSAASSLAMQELNLKSEISDKLATKLQKLLMLSSHRRLLLSKLVHIAPD 199

Query: 199 LSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPLPCLQVSSRELIVDRPLK 258
              PPNFRSRLCNDYP+KF+TVDTSYGRALELV  DPELA  +P  +V  R LIVDRPLK
Sbjct: 200 FGFPPNFRSRLCNDYPDKFKTVDTSYGRALELVSSDPELANQMPSPEV-DRGLIVDRPLK 259

Query: 259 FNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKESLESEKRACAVVREVLGM 318
           F  L LR+GLNLKR HQ FLIKFR+ PDVCPYK  +  LA ES+E+EKRACAVVREVLG+
Sbjct: 260 FKRLNLRRGLNLKRRHQGFLIKFRESPDVCPYKMSSDYLASESIEAEKRACAVVREVLGL 319

Query: 319 MIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQRDSVFLVEGFNDKGHLIE 378
            +EKRTLIDHLTHFRK+F LPNKLR +IVRHPELFYVS+KG RDSVFLVE +ND G L++
Sbjct: 320 TVEKRTLIDHLTHFRKEFSLPNKLRDLIVRHPELFYVSIKGMRDSVFLVEAYNDNGDLLD 379

Query: 379 KDETLAIKNQWMKLLMEGKRMRREKRK--AQIYDSRYGNDHENHSHDHEMETDYDDDYED 438
           KDE L I+ + + L+ EGKR+RRE+R+  A + D       ++     + ++D DD+YED
Sbjct: 380 KDERLVIRERLIDLIQEGKRIRRERRRKGATLGDKSVEEKRKDDETIDDYDSDLDDEYED 439

Query: 439 GFESLFQYEDL------DFEDEESDLPSNRSNGDFWT 467
           GFE+LF  EDL      D ED++ +   N  + ++W+
Sbjct: 440 GFENLFDSEDLGVEYHFDEEDDDDEAGVNGESVEYWS 474

BLAST of Carg22432 vs. TAIR 10
Match: AT4G01037.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 229.9 bits (585), Expect = 4.2e-60
Identity = 161/439 (36.67%), Postives = 232/439 (52.85%), Query Frame = 0

Query: 45  KTCYTLTP-RASVKIVRSRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGY 104
           KT   + P RA+VK  +    D    +  K + V  +  +L+S+P   + +  L K R  
Sbjct: 64  KTRVVVEPVRAAVKRRKELTFDSVVQRDKKLKLVLNIRKILVSQPDRMMSLRGLGKYRRD 123

Query: 105 LSLPKPRSLLSMIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLK 164
           L L K R  ++++ +YP +FE+             +    L  ++T  A  L   +  ++
Sbjct: 124 LGLKKRRRFIALLRKYPGVFEI-----------VEEGAYSLRFKMTSEAERLYLDEMRIR 183

Query: 165 LVISNTLAEKLQKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSY 224
             + + L  KL+KL+M+S   RILL K+ HL  DL +P  FR  +C  YP+ FR V T  
Sbjct: 184 NELEDVLVVKLRKLVMMSIDKRILLEKISHLKTDLGLPLEFRDTICQRYPQYFRVVPTPR 243

Query: 225 GRALELVFWDPELAKPLPCL--------QVSSRELIVDRPLKFNLLKLRKGLNLKRAHQE 284
           G ALEL  WDPELA     L        +   R LI+DRP KFN +KL +GLNL ++   
Sbjct: 244 GPALELTHWDPELAVSAAELSEDDNRTRESEERNLIIDRPPKFNRVKLPRGLNLSKSETR 303

Query: 285 FLIKFRDLPDVCPYKTPASELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDF 344
            + +FRD+  + PYK   S L   +LE EK AC V+ E+L +  EKRTL+DHLTHFR++F
Sbjct: 304 KISQFRDMQYISPYK-DFSHLRSGTLEKEKHACGVIHELLSLTTEKRTLVDHLTHFREEF 363

Query: 345 GLPNKLRGMIVRHPELFYVSLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLL--- 404
               +LRGM++RHP+LFYVSLKG+RDSVFL E + +   LI+KD    +K +   L+   
Sbjct: 364 RFSQQLRGMLIRHPDLFYVSLKGERDSVFLREAYRN-SELIDKDPLTLVKEKMRALVSVP 423

Query: 405 ----MEGKRMRREKRKAQIYDSRYGNDHENHSHDHEMETDYDD--DYEDGFESLFQYEDL 464
                 G R   E R+ +I  S    + E    D E  +D D   + EDG       +D 
Sbjct: 424 RFPRRGGPRKDEEGREVEIDGSDADGEEEEEESDAEEWSDVDGYLEGEDGGND----DDG 483

BLAST of Carg22432 vs. TAIR 10
Match: AT5G48040.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 128.3 bits (321), Expect = 1.7e-29
Identity = 115/387 (29.72%), Postives = 186/387 (48.06%), Query Frame = 0

Query: 56  VKIVRSRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSM 115
           +K V+ R LD   V+    R V  L+ ++ + P   +PI  L   RG L LP+   L + 
Sbjct: 37  LKWVKDRELDAVVVREKHLRAVCNLVSVISASPDLRLPIFKLLPHRGQLGLPQELKLSAF 96

Query: 116 IHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQ 175
           I RYP+IF    + +       T +    C  LT     L  ++ ++  V    +  +L 
Sbjct: 97  IRRYPNIF----VEHCYWDSAGTSV---PCFGLTRETIDLYYEEVDVSRVNERDVLVRLC 156

Query: 176 KLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYG-RALELVFWDP 235
           KLLML+    + L  + HL  DL +P ++R  L   +P+ F  V  S     L+L+ WD 
Sbjct: 157 KLLMLTCERTLSLHSIDHLRWDLGLPYDYRDSLITKHPDLFSLVKLSSDLDGLKLIHWDE 216

Query: 236 ELAKPLPCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAS 295
            LA      Q+  RE + +       +K  +G  LKR   E+L +++ LP   PY   AS
Sbjct: 217 HLA----VSQMQLREDVGNDERMAFPVKFTRGFGLKRKSIEWLQEWQRLPYTSPY-VDAS 276

Query: 296 ELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYV 355
            L   +  SEKR   V  E+L + I K+T   ++++ RK F LP K   +  RHP +FY+
Sbjct: 277 HLDPRTDLSEKRNVGVFHELLHLTIGKKTERKNVSNLRKPFALPQKFTKVFERHPGIFYI 336

Query: 356 SLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEG--KRMRREKRKAQIYDSRY 415
           S+K    +V L E + D+ HLIEK   + ++ ++  ++ EG   R R   +K+   D   
Sbjct: 337 SMKCDTQTVILREAY-DRRHLIEKHPLVEVREKFANMMNEGFLDRSRGLYQKSVEADLEK 396

Query: 416 GNDHENHSHDHEMETDYDDDYEDGFES 440
            N   ++    + E + D++   G++S
Sbjct: 397 NNIKTSYPVWSDEEEELDNNLISGYDS 410

BLAST of Carg22432 vs. TAIR 10
Match: AT3G63090.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 125.9 bits (315), Expect = 8.6e-29
Identity = 107/359 (29.81%), Postives = 176/359 (49.03%), Query Frame = 0

Query: 54  ASVKIV--RSRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLP-KPR 113
           +S+K+V  +   LD    +  + +   +++  +L++P   IP+  L K R  L L  K +
Sbjct: 32  SSLKVVWKKDTRLDEAIEQDKRYKLCARVVKEVLNEPGQVIPLRYLEKRRERLRLTFKAK 91

Query: 114 SLLSMIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTL 173
           S + M    PS+FE++     P      K  P   +R TP   +   ++  +       L
Sbjct: 92  SFVEM---NPSLFEIYYDRIKP------KSDPVQFLRPTPRLRAFLDEEQRIYSENEPLL 151

Query: 174 AEKLQKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFR-TVDTSYGRA-LE 233
             KL +LLM++    I   KLVH+  D   P +F  +L   YP  FR T     G++ LE
Sbjct: 152 VSKLCQLLMMAKDKVISADKLVHVKRDFGFPNDFLMKLVQKYPNYFRLTGLPEEGKSFLE 211

Query: 234 LVFWDPELAKPLPCLQVSSRELIVDRPLKFNL-LKLRKGLNLKRAHQEFLIKFRDLPDVC 293
           LV W+P+ AK    L+     L     ++ N  +KL  G  L++  +E+   + +   + 
Sbjct: 212 LVSWNPDFAKSKIELRAEDETLKTGVRIRPNYNVKLPSGFFLRKEMREWTRDWLEQDYIS 271

Query: 294 PYKTPASELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVR 353
           PY+   S L + S E EKR   VV E+L + + KR  +  L  F  +F   N    +  R
Sbjct: 272 PYE-DVSHLDQASKEMEKRTVGVVHELLSLSLLKRVPVPTLGKFCDEFRFSNAFSSVFTR 331

Query: 354 HPELFYVSLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQ 407
           H  +FY+SLKG   +  L E + D   L+++D  LAIK+++++LL EG + R+++ K Q
Sbjct: 332 HSGIFYLSLKGGIKTAVLREAYKD-DELVDRDPLLAIKDKFLRLLEEGWQERKDRLKLQ 379

BLAST of Carg22432 vs. TAIR 10
Match: AT5G21970.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 119.4 bits (298), Expect = 8.0e-27
Identity = 114/407 (28.01%), Postives = 187/407 (45.95%), Query Frame = 0

Query: 60  RSRPLDRHAVKHNKTRFVQKLIIL---LLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMI 119
           R + +    +   K +   K+I L   L  +    + +    + R  ++LPKP  +   I
Sbjct: 54  REKRVQELEIATEKWKIASKVIFLMEVLKGERDMIMTVRSFEQYRRQINLPKPHKISDFI 113

Query: 120 HRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQK 179
            + P +FEL+                 L   LT     L  +   L     +  AE + +
Sbjct: 114 RKSPKLFELYK-----------DQRGVLWCGLTEGGEDLLDEHDKLLEENGDKAAEHVTR 173

Query: 180 LLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRA-LELVFWDPE 239
            LM+S   ++ L K+VH   D  +P +FR     ++P+ F+ V    G   LELV W+P 
Sbjct: 174 CLMMSVDKKLPLDKIVHFRRDFGLPLDFRINWVYNFPQHFKVVKLGDGEEYLELVSWNPA 233

Query: 240 LAKPLPCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEF------LIKFRDLPDVCPY 299
            A  +  L+  +  +  D   K  +L L   +    ++++       +  F+    + PY
Sbjct: 234 WA--ITELEKKTLGITEDCEHKPGMLSLAFPMKFPPSYKKMYRYRGKIEHFQKRSYLSPY 293

Query: 300 KTPASELAKESLESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHP 359
              A  L   S E +KRA AV+ E+L   +EKR + DHLTHFR++F +P KL  + ++H 
Sbjct: 294 -ADARGLEAGSKEFDKRAIAVMHELLSFTLEKRLVTDHLTHFRREFVMPQKLMRIFLKHC 353

Query: 360 ELFYVSLKGQRDSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYD 419
            +FYVS +G+R SVFL EG+ +   LIEK   +     W + L++    R  KR  Q Y 
Sbjct: 354 GIFYVSERGKRFSVFLTEGY-EGPELIEKCPLIL----WKEKLLKFTGYRGRKRDIQTY- 413

Query: 420 SRYGNDHENHSHDHEMET-DYDDDYEDGFESLFQYEDLDFEDEESDL 456
               +D  +      +E+   D+D   GFE    Y+D+  +D+E D+
Sbjct: 414 ----SDTLDMEERELLESGSGDEDLSVGFEKDGDYDDVVTDDDEMDI 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022955645.11.5e-280100.00protein WHAT'S THIS FACTOR 1 [Cucurbita moschata] >XP_022955646.1 protein WHAT'S... [more]
KAG6582056.18.0e-27999.79Protein WHAT'S THIS FACTOR 1-like, chloroplastic, partial [Cucurbita argyrosperm... [more]
XP_023527715.17.7e-27498.14protein WHAT'S THIS FACTOR 1 [Cucurbita pepo subsp. pepo][more]
XP_022979770.11.7e-27398.14protein WHAT'S THIS FACTOR 1 [Cucurbita maxima] >XP_022979771.1 protein WHAT'S T... [more]
XP_038904265.11.9e-25190.31protein WHAT'S THIS FACTOR 1, chloroplastic [Benincasa hispida] >XP_038904305.1 ... [more]
Match NameE-valueIdentityDescription
B6TTV81.4e-6036.90Protein WHAT'S THIS FACTOR 1, chloroplastic OS=Zea mays OX=4577 GN=WTF1 PE=1 SV=... [more]
Q65XL52.7e-5936.66Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Oryza sativa subsp. japon... [more]
A0MFS55.9e-5936.67Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q689D64.6e-1925.73Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=RPD1 PE=1... [more]
Q9ZUZ65.1e-1824.50Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=W... [more]
Match NameE-valueIdentityDescription
A0A6J1GVP17.1e-281100.00protein WHAT'S THIS FACTOR 1 OS=Cucurbita moschata OX=3662 GN=LOC111457587 PE=4 ... [more]
A0A6J1IUA58.4e-27498.14protein WHAT'S THIS FACTOR 1 OS=Cucurbita maxima OX=3661 GN=LOC111479376 PE=4 SV... [more]
A0A0A0KU861.5e-24688.66PORR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015830 PE=4 S... [more]
A0A5A7TP081.8e-24488.87Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A1S3BYB21.2e-24388.87protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo OX=3656 GN=LOC103494529 PE=4... [more]
Match NameE-valueIdentityDescription
AT5G62990.11.3e-14160.61Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT4G01037.14.2e-6036.67Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT5G48040.11.7e-2929.72Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT3G63090.18.6e-2929.81Ubiquitin carboxyl-terminal hydrolase family protein [more]
AT5G21970.18.0e-2728.01Ubiquitin carboxyl-terminal hydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021099Plant organelle RNA recognition domainPFAMPF11955PORRcoord: 59..397
e-value: 7.2E-93
score: 311.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 405..429
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 405..426
NoneNo IPR availablePANTHERPTHR31476:SF2UBIQUITIN CARBOXYL-TERMINAL HYDROLASE FAMILY PROTEINcoord: 39..448
IPR045040PORR familyPANTHERPTHR31476PROTEIN WHAT'S THIS FACTOR 1 HOMOLOG, CHLOROPLASTICcoord: 39..448

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22432-RACarg22432-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008233 peptidase activity
molecular_function GO:0003723 RNA binding