Carg22399 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22399
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionDUF21 domain-containing protein At1g55930, chloroplastic-like
LocationCarg_Chr01: 9210322 .. 9214598 (-)
RNA-Seq ExpressionCarg22399
SyntenyCarg22399
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCACAAACCTGCTTACTTCGTGCCTGGTAATTCTTAACTCAAATGTCACTTTTTGGTAAAAATTGATAAGCTGATGTAAGTACATATAGATGGTCTTTATTGCTTATTTACAGGCAACAAATTCCACTGATCTTTCCAACAAGCTCAATACTTCTTTCTTATATGTATACTGATGGAACCTCAACTCATTTTTGCATTCTAAATGCAGATATCTGATTCCCTCACTTCACATTCCTAGGCTTAAACATTCCAATGCTGGCACCCTAACACCCAACTACTGTTGTGTAAATTGTCCCTGCTAAATTTTATAGGCTGGAAAGTTTTGCTTAGTTCCTGTAGGTATTATGTTAGGTTGCTGGATGTGGTGGGCTTTTACAACATTTTAAAATTAAAACTATGTAATGATGAACTTTTTCTTCTTGATATTTCGAATTTTGTCATTCAAATGCTGGAAGATAATAATCGATCCATTCATGATCAGTAACTTGATAGGACCTGTAAGGACCCAGATCCACCGCTAGCAGATATTGTTCTCTTTGGGCTTTCCTTTTCGGGCTTCCCCTCAAGGCTTTAAAACGTGTCCGCTAGGGGAAGGTTTCCACACCCTTGTAAATGGTGGTTTGTTCTCCTCCCCAACCAATGTGGGACATCACAATCCACCCCCCTTCAGGCTCAGCGTCCTCGCTGGCCCTCTTCCTTCCTCCAATCGATATGGGACCGCCCCCAAATCCACCCCCCCTTTGGGGTCCAGCATCCTTACTGACACACCGTCTCGTGTCTACCCCCTTCAGGGAACAGCGAGAAGGCTGGCACATCGTCTGGTGTCTGACTCTGATACCATTTGTAATGACCCAGATCTACCGCTAGCAGATATTGTCCTCTTTGGGCTTTCCCTTTCGGGCTTCCCCTCAAGGCTTTAAAACACGTCTGCTAGGAGAAGGTTTCCACACCCTTATAAATGGTGGTTTGTTCTCCTCCCCAACCAATGCGGGACATCACAGGACCTCTAAGACATTGAAATATAAATGTAATGATTTTGAACTTTTATAAAGTTTTTTTTAAAGAAAATTAATTTACAGCCATCGGCCTCCATAGAATAAGACATTGTAGCCTGGATTATATATATCTATATCTATATATATACCTATGTGTGTATATATAAATATATATGTGTACATGCATGCATTCATGTATATAAACTTACGAGTTACACGGCATTGGTAAATTGTGTGCGCTAGGTGAAGGTTTTAGTCTGTCTGCATGCAAATTATACTTGAGTTTAACCACAAGTTGGGAACTTGAGATTGTACTACATACATTTTTTTGCTTTCATTTTCTGGTTGTCATTCCATAGTCTTCCTAATTATCATCATGTCAAAGTTGTTTATGTGTTTTCCAGATTCAATGTCAGTCTGGAATCTTCTCCGAGAGTTTCGTATCCGGAAAGTTCACATGGCTGTTGTGCTTAACGAATATGGCGGCACAGTTGGAGTATGTGTACTCACCATATCTTTGTTTTTATCTTATGCAATGATTTATGCTATTTTAAACCAAGATCGCATACAGAAATTTAAGGAATCCCACTTTTCATTTGTAACTCATATATTTTCTCCTAGAAGTAGTAGTCAGGAGCCCTTTTCCACTGCATAACATTAATTCTCAGAATTAAAATCTTATTGCAATAGAGAGCAGCTAGTAAGGCAAAAGTTAACACAAGGAAATATCGATCTGTGGCCTGTATCCTCATGCGTTCGGATTTCATGAACTACCAAGTTAAATGCAATCAGACATTGGCAATGGCAGGCACTTATGTGGAGTTTCTAATTTGGGTCTTGAGGCTAGCTTTTGTATGAGTGGGATACTTGAATTCTTGGGAAGGAAAGAGAATACAGAAAGAAAAGGAAGATAAAGGGGACAAAAGAAAAGACTTGGAGGAGGAGTGTGATGTCCCACATTGGTTGGGGAGGAGAACAAATCACCCTTTATGAGAGTGTGGCAACCTTCCCCTAACAGGGGGAAGCCCGAAAGGGAAAGCCAAAGAGGACAATATCTGCTAGCGGTGGATCTAGGCCGTTACAAATGGTATCAGAGTCAGACATTGGACGATGTGCCAGCCTTCTCACTGTTCCCCGAAGGGGGTAGACATGAGGCGGTGTGCCAGTAAGGACGCTGGCCCCAAAGGGGGATGGATTTGGTGGTGGTCTCACATTGATTAGAGAAAGGAATGAGTGCCAGCGAGGACGCTGGGCCCGAAGGGGGGTGGATTGTGATGTCCCACGTTGGTTGGGGAGGAGAACAAATCACCCTTTATAAGGGTGTGGAAATCTTCCCCTAGTAGACGCATTTTAAAGCCTTGAGGGAAAGCCAAAGAGGACAATATCTGCTAGCGGTGAATCTGGGGCGTTATAGGGGGGGGGGGGGGGGGCGACGGTGGAGAAAGTTGTTTAAGGGATTTGAATTGTTTTTCCGGAGCTTCTAATGATATTTTTTTAATAACGGAGTAGTTTCTCTTATAAATGGACCTTGTTCTTTCAGTGCATTTTGCTCGTTTGTTGAATTGATTTGTACTGTTTTTCCTTATATTACAAAAGGAAAAAGAAAATGGGTAGTTGAGAGTTGCATAGTCTCAATACTCTACCGTAACTCGTAAAATCGTTTCTTTTTAGTGAATATCTGATCTTTTACTTTTCAAATTGAATCAGATTGTAACACTGGAAGATGTGGTTGAGGAAATTGTAGGTGAAATCTTCGACGAAAACGATTCAAAGGTAAATGTCGCTGTCATTTTTCTTTACACCGAATGCACACTGTCACTTTATTTTCTTGAATGTGCCTTTTGATTCTCACAAGTGATTCTTGGCTATAGAAAACATCTGTGTTCATATTATTAGGTGCTATTATATTGTTATCTAAACTAAAAAGTATAAGCACAGCCATATTGTTTGTCATGCACTCGTGCTATTATGTTTTTAATGATTTTTCTTTTGTTTTTCATTTTCAAAAAACTGAAGGAGGAGATCCAGAAGAAAACTGGCTACATTGTGATGCGAGCCGATGGAGTATATGATGTCGATGCAAATACCGCAATCGATCAGCTCTCTGAAGATCTAAATATCAAAATGCCAGAGGTAGTTTACCTTTTATTATCAAAATGCCTCTTCCCTTCACCCCTTATTTCTGTTCGGTTTTTTTATGAGATACGTTTTCATTGATGTATGAAATTTACTAAAGGGATGGATCATGAGAGTTCCAAAAATTTTCCCGATTAGTCAAAATGATCGATCGTAACTATATGAAGCGAGAAGGTTAGAACCTTTACACCAAGACAGCAAAATAAATAACAAGATAAAAGAAAACATCAAAAGATTGTTTCTTGTCCAGAATTATTCTTTGATTTCTTTCCTTACAAAGGACCCAAAATAAGCATACGTTTTGTACAAATGTACCTACGCTTGTTCGTATACGTTTTAAATTGTATTTTGAATGCTTATTACTAAATTCTTACATATTACTTCTTGCCACTTTAGGGTCATCAATACGAAACGGTGTCGGGTTTTGTTTGTGAGGCTTTTGGATATATCCCAAGGACGGGTGAAAGCGTTAAAGTGGTCCTTGAAAAGGAAGACGAAGAAGCAGAAGAGTCAAATTCCGAAAACAAAAATCAGAAGCAAAAACGCCTAATCTTCAATATCGAGGTATAATTTTATTTCTGTATTGCCTACAATTTCATTTGGTATACTTTGTTGGGAAGTACCAAAAAGCTTCGGAAAACCGTTCGGCCTATCATTTCTCAATTTTTGCAATTTTATCATCTGCCACTTTCATAGTTCATCTGCATATGATACCGTTTTCGTCGCTTCCTTTTAGTCAATGATGCGATTCCTTAACTTTCATATTCGGATTTTAGTATTAAGCTGAGTATAGGCTGTTTAACATTGGTATCTACTCTGGCTGTTAATTGTACTTCTTTTTCAGCATTCTTACACTGTCTCGTTCGGTTTCTTTAATTTCGGAGTAGTAGGGTTGATATGTTGGACCTTTGTGCAGATATTAGCAGGAAATGCTAGAAAGGTTAGTGCTGTTCGGTTTGAACGGGTGAATGACGATAAAGGTGAGGTGGCTCATCTTGTCCCTAAAGTCATGGAGAAGAAATGGAGCAGTAATGGGGAGTCTGGTAGTGTAGAAAATGATAATATATTATCAGAGAGACTTGATGACAGCCTCTCTAGATTGCACCAAAACGATGATGATCATAGCAGTGATAGAAATTAG

mRNA sequence

ATGGCCCACAAACCTGCTTACTTCGTGCCTGATTCAATGTCAGTCTGGAATCTTCTCCGAGAGTTTCGTATCCGGAAAGTTCACATGGCTGTTGTGCTTAACGAATATGGCGGCACAGTTGGAATTGTAACACTGGAAGATGTGGTTGAGGAAATTGTAGGTGAAATCTTCGACGAAAACGATTCAAAGGGTCATCAATACGAAACGGTGTCGGGTTTTGTTTGTGAGGCTTTTGGATATATCCCAAGGACGGGTGAAAGCGTTAAAGTGGTCCTTGAAAAGGAAGACGAAGAAGCAGAAGAGTCAAATTCCGAAAACAAAAATCAGAAGCAAAAACGCCTAATCTTCAATATCGAGATATTAGCAGGAAATGCTAGAAAGGTTAGTGCTGTTCGGTTTGAACGGGTGAATGACGATAAAGGTGAGGTGGCTCATCTTGTCCCTAAAGTCATGGAGAAGAAATGGAGCAGTAATGGGGAGTCTGGTAGTGTAGAAAATGATAATATATTATCAGAGAGACTTGATGACAGCCTCTCTAGATTGCACCAAAACGATGATGATCATAGCAGTGATAGAAATTAG

Coding sequence (CDS)

ATGGCCCACAAACCTGCTTACTTCGTGCCTGATTCAATGTCAGTCTGGAATCTTCTCCGAGAGTTTCGTATCCGGAAAGTTCACATGGCTGTTGTGCTTAACGAATATGGCGGCACAGTTGGAATTGTAACACTGGAAGATGTGGTTGAGGAAATTGTAGGTGAAATCTTCGACGAAAACGATTCAAAGGGTCATCAATACGAAACGGTGTCGGGTTTTGTTTGTGAGGCTTTTGGATATATCCCAAGGACGGGTGAAAGCGTTAAAGTGGTCCTTGAAAAGGAAGACGAAGAAGCAGAAGAGTCAAATTCCGAAAACAAAAATCAGAAGCAAAAACGCCTAATCTTCAATATCGAGATATTAGCAGGAAATGCTAGAAAGGTTAGTGCTGTTCGGTTTGAACGGGTGAATGACGATAAAGGTGAGGTGGCTCATCTTGTCCCTAAAGTCATGGAGAAGAAATGGAGCAGTAATGGGGAGTCTGGTAGTGTAGAAAATGATAATATATTATCAGAGAGACTTGATGACAGCCTCTCTAGATTGCACCAAAACGATGATGATCATAGCAGTGATAGAAATTAG

Protein sequence

MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDENDSKGHQYETVSGFVCEAFGYIPRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKGEVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN
Homology
BLAST of Carg22399 vs. NCBI nr
Match: KAG7037339.1 (DUF21 domain-containing protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 376.7 bits (966), Expect = 1.2e-100
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN
Sbjct: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60

Query: 61  DSKGHQYETVSGFVCEAFGYIPRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEI 120
           DSKGHQYETVSGFVCEAFGYIPRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEI
Sbjct: 61  DSKGHQYETVSGFVCEAFGYIPRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEI 120

Query: 121 LAGNARKVSAVRFERVNDDKGEVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSR 180
           LAGNARKVSAVRFERVNDDKGEVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSR
Sbjct: 121 LAGNARKVSAVRFERVNDDKGEVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSR 180

Query: 181 LHQNDDDHSSDRN 194
           LHQNDDDHSSDRN
Sbjct: 181 LHQNDDDHSSDRN 193

BLAST of Carg22399 vs. NCBI nr
Match: KAG6607762.1 (DUF21 domain-containing protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 357.5 bits (916), Expect = 7.5e-95
Identity = 193/232 (83.19%), Postives = 193/232 (83.19%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN
Sbjct: 425 MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 484

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       GHQYETVSGFVCEAFGYI
Sbjct: 485 DSKEEIQKKTGYIVMRADGVYDVDANTAIDQLSEDLNIKMPEGHQYETVSGFVCEAFGYI 544

Query: 121 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 180
           PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG
Sbjct: 545 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 604

Query: 181 EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN 194
           EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN
Sbjct: 605 EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN 656

BLAST of Carg22399 vs. NCBI nr
Match: XP_022940660.1 (putative DUF21 domain-containing protein At3g13070, chloroplastic [Cucurbita moschata])

HSP 1 Score: 357.5 bits (916), Expect = 7.5e-95
Identity = 193/232 (83.19%), Postives = 193/232 (83.19%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN
Sbjct: 425 MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 484

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       GHQYETVSGFVCEAFGYI
Sbjct: 485 DSKEEIQKKTGYIVMRADGVYDVDANTAIDQLSEDLNIKMPEGHQYETVSGFVCEAFGYI 544

Query: 121 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 180
           PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG
Sbjct: 545 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 604

Query: 181 EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN 194
           EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN
Sbjct: 605 EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN 656

BLAST of Carg22399 vs. NCBI nr
Match: XP_023525513.1 (putative DUF21 domain-containing protein At3g13070, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 354.8 bits (909), Expect = 4.8e-94
Identity = 191/232 (82.33%), Postives = 193/232 (83.19%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN
Sbjct: 425 MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 484

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       GHQYETVSGFVCEAFGYI
Sbjct: 485 DSKEEIQKKTGYIVMRADGVYDVDANTAIDQLSEDLNIKMPEGHQYETVSGFVCEAFGYI 544

Query: 121 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 180
           PRTGESVKVVLEKEDEEAEESNSENKN+KQKRLIFNIEILAGNARKVSAVRFERVNDDKG
Sbjct: 545 PRTGESVKVVLEKEDEEAEESNSENKNKKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 604

Query: 181 EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN 194
           EVAHLVPKVMEKKWSSNGESGSVENDNILSER+DDSLSRLHQNDDDHSSDRN
Sbjct: 605 EVAHLVPKVMEKKWSSNGESGSVENDNILSERVDDSLSRLHQNDDDHSSDRN 656

BLAST of Carg22399 vs. NCBI nr
Match: XP_022981408.1 (putative DUF21 domain-containing protein At3g13070, chloroplastic [Cucurbita maxima])

HSP 1 Score: 346.3 bits (887), Expect = 1.7e-91
Identity = 188/232 (81.03%), Postives = 192/232 (82.76%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN
Sbjct: 425 MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 484

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       GHQYETVSGFVCEAFGYI
Sbjct: 485 DSKEEIQKKTGYIVMRADGVYDVDANTAIDQLSEDLNIKMPEGHQYETVSGFVCEAFGYI 544

Query: 121 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 180
           PRTGES+KVVLEKEDEEAEESNSENKN+KQ+RLIFNIEILAGNARKVSAVRFERVND+KG
Sbjct: 545 PRTGESIKVVLEKEDEEAEESNSENKNKKQRRLIFNIEILAGNARKVSAVRFERVNDNKG 604

Query: 181 EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN 194
           EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQN DDHSSDRN
Sbjct: 605 EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQN-DDHSSDRN 655

BLAST of Carg22399 vs. ExPASy Swiss-Prot
Match: Q9LK65 (Putative DUF21 domain-containing protein At3g13070, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CBSDUFCH1 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 5.3e-59
Identity = 132/220 (60.00%), Postives = 147/220 (66.82%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGT+GIVTLEDVVEEIVGEIFDEN
Sbjct: 426 MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTIGIVTLEDVVEEIVGEIFDEN 485

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       G QYETVSGFVCEAFGYI
Sbjct: 486 DSKEEIQKKTGYIVMRDEGIYDVDANTSIDQLSEELNMKMPEGIQYETVSGFVCEAFGYI 545

Query: 121 PRTGESVKVVLEK----EDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVN 173
           P+TGESVKVVLEK    ED E EE   E +  K+K  I+ +EILAGNARKVSAVRFERVN
Sbjct: 546 PKTGESVKVVLEKESWEEDGEEEEGKQERQEPKEKNQIYRVEILAGNARKVSAVRFERVN 605

BLAST of Carg22399 vs. ExPASy Swiss-Prot
Match: Q84R21 (DUF21 domain-containing protein At1g55930, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CBSDUFCH2 PE=2 SV=2)

HSP 1 Score: 221.9 bits (564), Expect = 6.4e-57
Identity = 128/217 (58.99%), Postives = 150/217 (69.12%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPA+FVPDSMSVWNLLREFRIRKVHMAVVLNEYGGT+GIVTLEDVVEEIVGEIFDEN
Sbjct: 421 MAHKPAFFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTIGIVTLEDVVEEIVGEIFDEN 480

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       GHQYETVSGFVCEAFGYI
Sbjct: 481 DSKEEIQKKTGYIVMRAEGIYDVDANTSIDQLSEELNIKMAEGHQYETVSGFVCEAFGYI 540

Query: 121 PRTGESVKVVLEK----EDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVN 169
           P+TGESV VVLEK    E++E +E   E ++QK+K  I+ +EILAGNARKVSAVRFERV+
Sbjct: 541 PKTGESVTVVLEKENWEENDEQDEGKHERQDQKEKHQIYRLEILAGNARKVSAVRFERVS 600

BLAST of Carg22399 vs. ExPASy Swiss-Prot
Match: Q9KTE3 (Magnesium and cobalt efflux protein CorC OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=corC PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.6e-13
Identity = 43/120 (35.83%), Postives = 62/120 (51.67%), Query Frame = 0

Query: 4   KPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDENDSK 63
           +PA  VP+S  V  LL+EFR  + HMA+V++E+GG  G+VT+ED++EEIVG+I DE D +
Sbjct: 138 RPAVVVPESKRVDRLLKEFREERYHMAIVVDEFGGVSGLVTIEDILEEIVGDIEDEFDDE 197

Query: 64  ---------------------------------GHQYETVSGFVCEAFGYIPRTGESVKV 91
                                              + +TV G V  AFG++P  GE V +
Sbjct: 198 EQKDIRQLSKHTFSVKALTTIEDFNHTFGTKFSDEEVDTVGGLVMTAFGHLPARGEVVDI 257

BLAST of Carg22399 vs. ExPASy Swiss-Prot
Match: P0A2L4 (Magnesium and cobalt efflux protein CorC OS=Salmonella typhi OX=90370 GN=corC PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.3e-12
Identity = 41/120 (34.17%), Postives = 65/120 (54.17%), Query Frame = 0

Query: 4   KPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDE---- 63
           + A  VP+S  V  +L+EFR ++ HMA+V++E+GG  G+VT+ED++E IVGEI DE    
Sbjct: 140 RTAVVVPESKRVDRMLKEFRSQRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEE 199

Query: 64  ------------------------NDSKG-----HQYETVSGFVCEAFGYIPRTGESVKV 91
                                   ND+ G      + +T+ G V +AFG++P  GE++ +
Sbjct: 200 DDIDFRQLSRHTWTIRALASIEDFNDAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDI 259

BLAST of Carg22399 vs. ExPASy Swiss-Prot
Match: P0A2L3 (Magnesium and cobalt efflux protein CorC OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=corC PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.3e-12
Identity = 41/120 (34.17%), Postives = 65/120 (54.17%), Query Frame = 0

Query: 4   KPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDE---- 63
           + A  VP+S  V  +L+EFR ++ HMA+V++E+GG  G+VT+ED++E IVGEI DE    
Sbjct: 140 RTAVVVPESKRVDRMLKEFRSQRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEE 199

Query: 64  ------------------------NDSKG-----HQYETVSGFVCEAFGYIPRTGESVKV 91
                                   ND+ G      + +T+ G V +AFG++P  GE++ +
Sbjct: 200 DDIDFRQLSRHTWTIRALASIEDFNDAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDI 259

BLAST of Carg22399 vs. ExPASy TrEMBL
Match: A0A6J1FR89 (putative DUF21 domain-containing protein At3g13070, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111446184 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 3.6e-95
Identity = 193/232 (83.19%), Postives = 193/232 (83.19%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN
Sbjct: 425 MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 484

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       GHQYETVSGFVCEAFGYI
Sbjct: 485 DSKEEIQKKTGYIVMRADGVYDVDANTAIDQLSEDLNIKMPEGHQYETVSGFVCEAFGYI 544

Query: 121 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 180
           PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG
Sbjct: 545 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 604

Query: 181 EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN 194
           EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN
Sbjct: 605 EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN 656

BLAST of Carg22399 vs. ExPASy TrEMBL
Match: A0A6J1J205 (putative DUF21 domain-containing protein At3g13070, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111480538 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 8.3e-92
Identity = 188/232 (81.03%), Postives = 192/232 (82.76%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN
Sbjct: 425 MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 484

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       GHQYETVSGFVCEAFGYI
Sbjct: 485 DSKEEIQKKTGYIVMRADGVYDVDANTAIDQLSEDLNIKMPEGHQYETVSGFVCEAFGYI 544

Query: 121 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 180
           PRTGES+KVVLEKEDEEAEESNSENKN+KQ+RLIFNIEILAGNARKVSAVRFERVND+KG
Sbjct: 545 PRTGESIKVVLEKEDEEAEESNSENKNKKQRRLIFNIEILAGNARKVSAVRFERVNDNKG 604

Query: 181 EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQNDDDHSSDRN 194
           EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQN DDHSSDRN
Sbjct: 605 EVAHLVPKVMEKKWSSNGESGSVENDNILSERLDDSLSRLHQN-DDHSSDRN 655

BLAST of Carg22399 vs. ExPASy TrEMBL
Match: A0A6J1CDZ8 (DUF21 domain-containing protein At1g55930, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111010379 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.4e-85
Identity = 177/233 (75.97%), Postives = 186/233 (79.83%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN
Sbjct: 425 MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 484

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       GHQYETVSGFVCEAFGYI
Sbjct: 485 DSKEEIQKKTGYIVMRADGIYDVDANTAIDQLSEDLNIKMPEGHQYETVSGFVCEAFGYI 544

Query: 121 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 180
           PRTGE+VKVVLEKEDEE EESN+ENK QK++ L+FNIEILAGNARKVSAVRFERVNDD G
Sbjct: 545 PRTGETVKVVLEKEDEEEEESNAENKIQKERHLVFNIEILAGNARKVSAVRFERVNDDNG 604

Query: 181 EVAHLVPKVMEKKWSSNGESGSVENDNIL-SERLDDSLSRLHQNDDDHSSDRN 194
           EVAHLVPKV++KKWSSNGESGSVENDN+L SERLDDSLS  HQNDD +SSDRN
Sbjct: 605 EVAHLVPKVVKKKWSSNGESGSVENDNLLSSERLDDSLSSEHQNDDHNSSDRN 657

BLAST of Carg22399 vs. ExPASy TrEMBL
Match: A0A1S3CI33 (DUF21 domain-containing protein At1g55930, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501228 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.2e-83
Identity = 178/233 (76.39%), Postives = 185/233 (79.40%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN
Sbjct: 425 MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 484

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       GHQYETVSGFVCEAFGYI
Sbjct: 485 DSKEEIQKKTGYIVMRADGVYDVDANTAIDQLSEDLNIKMPEGHQYETVSGFVCEAFGYI 544

Query: 121 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 180
           PRTGESVKVVLEKED+E EESN ENKNQK++ LIFNIEILAGNARKVSAVRFERVNDD G
Sbjct: 545 PRTGESVKVVLEKEDDE-EESNPENKNQKERHLIFNIEILAGNARKVSAVRFERVNDDNG 604

Query: 181 EVAHLVPKVMEKKWSSNGESGSVENDNIL-SERLDDSLSRLHQNDDDHSSDRN 194
           EVAHLVPKVM+KKWSSN ESGSVENDN+L SE +D+SLSR HQN DDHSSDRN
Sbjct: 605 EVAHLVPKVMKKKWSSNDESGSVENDNLLSSEGVDESLSREHQN-DDHSSDRN 655

BLAST of Carg22399 vs. ExPASy TrEMBL
Match: A0A1S3CIL7 (putative DUF21 domain-containing protein At3g13070, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501228 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.2e-83
Identity = 178/233 (76.39%), Postives = 185/233 (79.40%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN
Sbjct: 233 MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 292

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       GHQYETVSGFVCEAFGYI
Sbjct: 293 DSKEEIQKKTGYIVMRADGVYDVDANTAIDQLSEDLNIKMPEGHQYETVSGFVCEAFGYI 352

Query: 121 PRTGESVKVVLEKEDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVNDDKG 180
           PRTGESVKVVLEKED+E EESN ENKNQK++ LIFNIEILAGNARKVSAVRFERVNDD G
Sbjct: 353 PRTGESVKVVLEKEDDE-EESNPENKNQKERHLIFNIEILAGNARKVSAVRFERVNDDNG 412

Query: 181 EVAHLVPKVMEKKWSSNGESGSVENDNIL-SERLDDSLSRLHQNDDDHSSDRN 194
           EVAHLVPKVM+KKWSSN ESGSVENDN+L SE +D+SLSR HQN DDHSSDRN
Sbjct: 413 EVAHLVPKVMKKKWSSNDESGSVENDNLLSSEGVDESLSREHQN-DDHSSDRN 463

BLAST of Carg22399 vs. TAIR 10
Match: AT3G13070.1 (CBS domain-containing protein / transporter associated domain-containing protein )

HSP 1 Score: 228.8 bits (582), Expect = 3.7e-60
Identity = 132/220 (60.00%), Postives = 147/220 (66.82%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGT+GIVTLEDVVEEIVGEIFDEN
Sbjct: 426 MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTIGIVTLEDVVEEIVGEIFDEN 485

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       G QYETVSGFVCEAFGYI
Sbjct: 486 DSKEEIQKKTGYIVMRDEGIYDVDANTSIDQLSEELNMKMPEGIQYETVSGFVCEAFGYI 545

Query: 121 PRTGESVKVVLEK----EDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVN 173
           P+TGESVKVVLEK    ED E EE   E +  K+K  I+ +EILAGNARKVSAVRFERVN
Sbjct: 546 PKTGESVKVVLEKESWEEDGEEEEGKQERQEPKEKNQIYRVEILAGNARKVSAVRFERVN 605

BLAST of Carg22399 vs. TAIR 10
Match: AT1G55930.1 (CBS domain-containing protein / transporter associated domain-containing protein )

HSP 1 Score: 221.9 bits (564), Expect = 4.6e-58
Identity = 128/217 (58.99%), Postives = 150/217 (69.12%), Query Frame = 0

Query: 1   MAHKPAYFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDEN 60
           MAHKPA+FVPDSMSVWNLLREFRIRKVHMAVVLNEYGGT+GIVTLEDVVEEIVGEIFDEN
Sbjct: 421 MAHKPAFFVPDSMSVWNLLREFRIRKVHMAVVLNEYGGTIGIVTLEDVVEEIVGEIFDEN 480

Query: 61  DSK---------------------------------------GHQYETVSGFVCEAFGYI 120
           DSK                                       GHQYETVSGFVCEAFGYI
Sbjct: 481 DSKEEIQKKTGYIVMRAEGIYDVDANTSIDQLSEELNIKMAEGHQYETVSGFVCEAFGYI 540

Query: 121 PRTGESVKVVLEK----EDEEAEESNSENKNQKQKRLIFNIEILAGNARKVSAVRFERVN 169
           P+TGESV VVLEK    E++E +E   E ++QK+K  I+ +EILAGNARKVSAVRFERV+
Sbjct: 541 PKTGESVTVVLEKENWEENDEQDEGKHERQDQKEKHQIYRLEILAGNARKVSAVRFERVS 600

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037339.11.2e-100100.00DUF21 domain-containing protein, chloroplastic, partial [Cucurbita argyrosperma ... [more]
KAG6607762.17.5e-9583.19DUF21 domain-containing protein, chloroplastic, partial [Cucurbita argyrosperma ... [more]
XP_022940660.17.5e-9583.19putative DUF21 domain-containing protein At3g13070, chloroplastic [Cucurbita mos... [more]
XP_023525513.14.8e-9482.33putative DUF21 domain-containing protein At3g13070, chloroplastic [Cucurbita pep... [more]
XP_022981408.11.7e-9181.03putative DUF21 domain-containing protein At3g13070, chloroplastic [Cucurbita max... [more]
Match NameE-valueIdentityDescription
Q9LK655.3e-5960.00Putative DUF21 domain-containing protein At3g13070, chloroplastic OS=Arabidopsis... [more]
Q84R216.4e-5758.99DUF21 domain-containing protein At1g55930, chloroplastic OS=Arabidopsis thaliana... [more]
Q9KTE32.6e-1335.83Magnesium and cobalt efflux protein CorC OS=Vibrio cholerae serotype O1 (strain ... [more]
P0A2L41.3e-1234.17Magnesium and cobalt efflux protein CorC OS=Salmonella typhi OX=90370 GN=corC PE... [more]
P0A2L31.3e-1234.17Magnesium and cobalt efflux protein CorC OS=Salmonella typhimurium (strain LT2 /... [more]
Match NameE-valueIdentityDescription
A0A6J1FR893.6e-9583.19putative DUF21 domain-containing protein At3g13070, chloroplastic OS=Cucurbita m... [more]
A0A6J1J2058.3e-9281.03putative DUF21 domain-containing protein At3g13070, chloroplastic OS=Cucurbita m... [more]
A0A6J1CDZ83.4e-8575.97DUF21 domain-containing protein At1g55930, chloroplastic-like OS=Momordica chara... [more]
A0A1S3CI333.2e-8376.39DUF21 domain-containing protein At1g55930, chloroplastic isoform X1 OS=Cucumis m... [more]
A0A1S3CIL73.2e-8376.39putative DUF21 domain-containing protein At3g13070, chloroplastic isoform X2 OS=... [more]
Match NameE-valueIdentityDescription
AT3G13070.13.7e-6060.00CBS domain-containing protein / transporter associated domain-containing protein... [more]
AT1G55930.14.6e-5858.99CBS domain-containing protein / transporter associated domain-containing protein... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 92..114
NoneNo IPR availableGENE3D3.90.1280.20coord: 1..63
e-value: 2.1E-27
score: 96.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 154..193
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..193
NoneNo IPR availablePANTHERPTHR22777:SF28BNAC03G73630D PROTEINcoord: 1..63
NoneNo IPR availablePANTHERPTHR22777:SF28BNAC03G73630D PROTEINcoord: 63..186
NoneNo IPR availablePANTHERPTHR22777HEMOLYSIN-RELATEDcoord: 63..186
coord: 1..63
NoneNo IPR availableSUPERFAMILY54631CBS-domain paircoord: 3..65
IPR005170Transporter-associated domainPFAMPF03471CorC_HlyCcoord: 42..136
e-value: 8.1E-13
score: 48.2
IPR000644CBS domainPFAMPF00571CBScoord: 3..53
e-value: 1.0E-6
score: 29.0
IPR000644CBS domainPROSITEPS51371CBScoord: 1..59
score: 11.62285
IPR044751Ion transporter-like, CBS domainCDDcd04590CBS_pair_CorC_HlyC_assoccoord: 4..50
e-value: 4.9882E-23
score: 87.166
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 55..140

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22399-RACarg22399-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding