Carg22174 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg22174
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPentatricopeptide repeat-containing protein
LocationCarg_Chr08: 2171780 .. 2174192 (+)
RNA-Seq ExpressionCarg22174
SyntenyCarg22174
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACCCCTCTTCTCTTCCGTTCAAAACCCATCTCGATTTTCAATCTTCCCGTTCAAACTCACCTTCAAAATCCTCCATTCTTTCCGACCACTCTCATCATCGGCAGCCGCCGGAAAACCCAAATGGAATTCACTCACCAATGTCTTCATTACCAACCCAACTCTTCTGATCCTACAATCATGCTCTTCCATGTTTCATTTGAAGCAAATTCAAGCTCACATGACTTGCACTGGCCTCATGAACCAAATCTTCCCGGCCAGCCGCGTCGTAGCCTTTTGTGCTCTCTCCGATGCCGGTGACATTCATTATGCCCATCTCGTTTTCGCTCAAATTGAAAACCCTAATTGCTTTTTGTGGAATACGATGATTAAGGGTTATTGCAGAGCTAAATGCCCTTCAATGGGGTTCTTGTTTTTCCGGCAAATGATTCGGAATCGAGTTGAATTGGACTGTGGGAGCTTTGTTTCTGCGCTCAAGGTGTGCGGCCAATTTTCCGGGAAAGCAATAAGAATGGCGGTGCATTCTGTGGTTTGGAAGTTGGGGTTTGGTTCTGAATTGCTGGTTCAAAATGGATTGATTCAGTACTATGTTGAAACTGGGTGTATTGGTTTTGCACGCCAAGTGTTTGATGAAAGTTCTGTGAAGGATGTTGTTACTTGGACGACGATGATCAATGGATACACAACGAACAATTGTTTGGATGAGGCTATAATGTTGTTCGATATGATGTCGTCGAGTAACGTTGAGCCGAATGAGGTCACTATGATTGCTTTGCTTTCTGCTTGCTCGCAGAAGGGAGATTATGTAATGGGGAGGTCACTTCATGAACTCATAAAAAGGAAGAACATAAGCTCCAGTGTTAATTTGGTCAATACCATGTTGGATATGTATGTGAAATGTGGCTATTTAGTAACTGCTAGAGAGATTTTTGACAACATGGAAAGAAGGGATGTTTTTTCTTGGACTAGCTTGCTTAATGGACTTGCTAAAAATGGAGATTTGGAAGCTGCAAGGAAGGTGTTTGATGAAATGCCTGAGAGAAATATTGTCTCTTGGAATGCTATGATTGCTGGTTATTCCCAAAACAGTCAGCCAATGGAAGCTTTGAAGCTATTTCACAACATGGTGGATGTTGTCGGATTGGTTCCAACCGAGGACACTTTGGCCTGTGTGCTTTCTGCTTGTGGCCAATTGGGATGCTTAGAAATGGGCAAATGGATTCATGACAACCATGTCAATAAAATGGGAATTCAATTGAGTTTGATTCTGAACAATGCAGTTATGGACATGTATGCCAAATGTGGGAGCATTGATGCAGCTGCAAAGCTCTTCCACTCCATTCCCGAGAAAAATCTAGTTTCTTGGAACACAATGATATCTGCATATGCATCTCATGGCCATGCCAAGAGAGCTCTCACTCTGTTCGATCAAATGAAACACTCGGGACTAAAACCAGATCACATCACCTTCATCAGCGTTCTATCAGCTTGCAGTTACGGTGGGTTTGTTTCGAAAGGTCGGGAGCATTTCGAAAGCATGGAGAATACCTTTGGAATAGAGCCAAAGAGGGAACACTACGCTTGTATGGTGGATTTGTTTAGTCGAGTTGGGTTGTTAAAAGAAGCTTACGAGCTAATATCGAAGATGCCGATCAAAGCAAGTGAAGGGGCTTGGGGAGCTTTGCTTGATGCTTGTAGGAAGCAGGGGAATGTGGAAATGGCTAAGTTAGCAGCTGGGAAGCTTTTGGAGTTGGATCCTGAGGACAGTGGGATTTATACTCTTTTGGCGAATATTTGTGCTAATGGGAAGAGATGGAACGATGTGAGAATGGTGAGAAGGATGATGAGAGAAAGAGGAGTCAAGAAGGTTCCTGGGCATAGCTTGATAGAGGTTGGAGGTACGTTTCATGAATTCTTAGTTGCTGATAATTCTCATCCTCATTCTTTAGAAGTTTATAGAGTGGTTAATGAATTGCTTCTGCTGTCTAGTTTGATAGATCTTGAACCTCTAGAAAATGATTAGTTCTTATATGATTTCCAGAAATTCTTAGTTAGATAATATGACTACAAGGTAGAATTTGAATTATGGAAGTATTTAGAGGTACTTGTGGTGGATGAGATGGTACCTATGAGATGCACGGCGGTTGGAGAGAAGAACGAAACATTTTTTTATAAAGGCGTGGAAACCTCTTTCCAGTAGACGCGTTTTAGGGAAAGCCCAAAGATGACAATATCAATTAGCGGTGGGTTTGAGTGGCTAGAATACCTTTCTACCTAGCTAATTGATTAGTTTAAAAGAATCAATCACTTTAAGAAACTCACGATAGATCTGGTCGTTGCGTCTAACAGCTCTCACGGCCGACGATGACAATCCTTCTAGATATGCATTTCTTTGCAGTAGTTCAAAGTGA

mRNA sequence

ATGAAACCCCTCTTCTCTTCCGTTCAAAACCCATCTCGATTTTCAATCTTCCCGTTCAAACTCACCTTCAAAATCCTCCATTCTTTCCGACCACTCTCATCATCGGCAGCCGCCGGAAAACCCAAATGGAATTCACTCACCAATGTCTTCATTACCAACCCAACTCTTCTGATCCTACAATCATGCTCTTCCATGTTTCATTTGAAGCAAATTCAAGCTCACATGACTTGCACTGGCCTCATGAACCAAATCTTCCCGGCCAGCCGCGTCGTAGCCTTTTGTGCTCTCTCCGATGCCGGTGACATTCATTATGCCCATCTCGTTTTCGCTCAAATTGAAAACCCTAATTGCTTTTTGTGGAATACGATGATTAAGGGTTATTGCAGAGCTAAATGCCCTTCAATGGGGTTCTTGTTTTTCCGGCAAATGATTCGGAATCGAGTTGAATTGGACTGTGGGAGCTTTGTTTCTGCGCTCAAGGTGTGCGGCCAATTTTCCGGGAAAGCAATAAGAATGGCGGTGCATTCTGTGGTTTGGAAGTTGGGGTTTGGTTCTGAATTGCTGGTTCAAAATGGATTGATTCAGTACTATGTTGAAACTGGGTGTATTGGTTTTGCACGCCAAGTGTTTGATGAAAGTTCTGTGAAGGATGTTGTTACTTGGACGACGATGATCAATGGATACACAACGAACAATTGTTTGGATGAGGCTATAATGTTGTTCGATATGATGTCGTCGAGTAACGTTGAGCCGAATGAGGTCACTATGATTGCTTTGCTTTCTGCTTGCTCGCAGAAGGGAGATTATGTAATGGGGAGGTCACTTCATGAACTCATAAAAAGGAAGAACATAAGCTCCAGTGTTAATTTGGTCAATACCATGTTGGATATTAGTTCAAAGTGA

Coding sequence (CDS)

ATGAAACCCCTCTTCTCTTCCGTTCAAAACCCATCTCGATTTTCAATCTTCCCGTTCAAACTCACCTTCAAAATCCTCCATTCTTTCCGACCACTCTCATCATCGGCAGCCGCCGGAAAACCCAAATGGAATTCACTCACCAATGTCTTCATTACCAACCCAACTCTTCTGATCCTACAATCATGCTCTTCCATGTTTCATTTGAAGCAAATTCAAGCTCACATGACTTGCACTGGCCTCATGAACCAAATCTTCCCGGCCAGCCGCGTCGTAGCCTTTTGTGCTCTCTCCGATGCCGGTGACATTCATTATGCCCATCTCGTTTTCGCTCAAATTGAAAACCCTAATTGCTTTTTGTGGAATACGATGATTAAGGGTTATTGCAGAGCTAAATGCCCTTCAATGGGGTTCTTGTTTTTCCGGCAAATGATTCGGAATCGAGTTGAATTGGACTGTGGGAGCTTTGTTTCTGCGCTCAAGGTGTGCGGCCAATTTTCCGGGAAAGCAATAAGAATGGCGGTGCATTCTGTGGTTTGGAAGTTGGGGTTTGGTTCTGAATTGCTGGTTCAAAATGGATTGATTCAGTACTATGTTGAAACTGGGTGTATTGGTTTTGCACGCCAAGTGTTTGATGAAAGTTCTGTGAAGGATGTTGTTACTTGGACGACGATGATCAATGGATACACAACGAACAATTGTTTGGATGAGGCTATAATGTTGTTCGATATGATGTCGTCGAGTAACGTTGAGCCGAATGAGGTCACTATGATTGCTTTGCTTTCTGCTTGCTCGCAGAAGGGAGATTATGTAATGGGGAGGTCACTTCATGAACTCATAAAAAGGAAGAACATAAGCTCCAGTGTTAATTTGGTCAATACCATGTTGGATATTAGTTCAAAGTGA

Protein sequence

MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQSCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLWNTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWKLGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIMLFDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK
Homology
BLAST of Carg22174 vs. NCBI nr
Match: KAG7025550.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 605.5 bits (1560), Expect = 2.5e-169
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60
           MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ
Sbjct: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60

Query: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120
           SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW
Sbjct: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120

Query: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWK 180
           NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWK
Sbjct: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWK 180

Query: 181 LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240
           LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML
Sbjct: 181 LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240

Query: 241 FDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 300
           FDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK
Sbjct: 241 FDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 300

BLAST of Carg22174 vs. NCBI nr
Match: XP_022960464.1 (pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 594.7 bits (1532), Expect = 4.3e-166
Identity = 293/300 (97.67%), Postives = 297/300 (99.00%), Query Frame = 0

Query: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60
           MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ
Sbjct: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60

Query: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120
           SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW
Sbjct: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120

Query: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWK 180
           NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQF+GKA+RMAVHSVVWK
Sbjct: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFAGKAVRMAVHSVVWK 180

Query: 181 LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240
           LGFGSELLVQNGLIQYYVE GCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML
Sbjct: 181 LGFGSELLVQNGLIQYYVEIGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240

Query: 241 FDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 300
           FD+MSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLD+  K
Sbjct: 241 FDLMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDMYVK 300

BLAST of Carg22174 vs. NCBI nr
Match: XP_023513845.1 (pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 589.3 bits (1518), Expect = 1.8e-164
Identity = 289/300 (96.33%), Postives = 295/300 (98.33%), Query Frame = 0

Query: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60
           MKPLFSSVQNPSRFSIFP K TFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ
Sbjct: 1   MKPLFSSVQNPSRFSIFPLKFTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60

Query: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120
           SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW
Sbjct: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120

Query: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWK 180
           NTMIKGYCRA+CPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQF+GKA+RMAVHSV+WK
Sbjct: 121 NTMIKGYCRARCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFAGKAVRMAVHSVIWK 180

Query: 181 LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240
           LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML
Sbjct: 181 LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240

Query: 241 FDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 300
           FD+M SSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLD+  K
Sbjct: 241 FDLMLSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDMYVK 300

BLAST of Carg22174 vs. NCBI nr
Match: XP_023004481.1 (pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 577.4 bits (1487), Expect = 7.1e-161
Identity = 286/300 (95.33%), Postives = 291/300 (97.00%), Query Frame = 0

Query: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60
           MKPLFSSVQN SRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ
Sbjct: 1   MKPLFSSVQNSSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60

Query: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120
           SCSSMFHLKQIQAHM CTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW
Sbjct: 61  SCSSMFHLKQIQAHMICTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120

Query: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWK 180
           NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALK CG F+GKAIRMAVHSVVWK
Sbjct: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKACGHFAGKAIRMAVHSVVWK 180

Query: 181 LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240
           LGFGS LLVQNGLIQ+YVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNN LDEA+ML
Sbjct: 181 LGFGSGLLVQNGLIQFYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNYLDEAVML 240

Query: 241 FDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 300
           FD+M SSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLD+  K
Sbjct: 241 FDLMLSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDMYVK 300

BLAST of Carg22174 vs. NCBI nr
Match: XP_038898970.1 (pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 489.2 bits (1258), Expect = 2.6e-134
Identity = 243/300 (81.00%), Postives = 263/300 (87.67%), Query Frame = 0

Query: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60
           MKP F S+QNPS FSIFP  L FKILHSFR LSSS +AGKPKWNSL+NVFI+NPTLLILQ
Sbjct: 1   MKPFFCSIQNPSHFSIFPLNLAFKILHSFRQLSSSTSAGKPKWNSLSNVFISNPTLLILQ 60

Query: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120
           SCSSM  LKQIQAHMT TGLMNQIFPASR++AFCALSD+GDIHYAHL+FAQIE+PNCF W
Sbjct: 61  SCSSMLQLKQIQAHMTRTGLMNQIFPASRLLAFCALSDSGDIHYAHLLFAQIEHPNCFFW 120

Query: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWK 180
           NTMIKGYCRA  PSMGF FFRQMIRNRV+LDCGSFV ALK C QF+GK +  AVHSV+WK
Sbjct: 121 NTMIKGYCRANHPSMGFSFFRQMIRNRVQLDCGSFVFALKACAQFAGKIVGTAVHSVIWK 180

Query: 181 LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240
           LGF SELLVQNGLIQ YVE GC+GFARQVFDESSVKDVVTWTTMINGYT NN LDEAI L
Sbjct: 181 LGFDSELLVQNGLIQNYVEIGCLGFARQVFDESSVKDVVTWTTMINGYTRNNWLDEAITL 240

Query: 241 FDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 300
           F+ M SS+VEPNEVTMIALLSA SQKGD VMGR LHE I+RK I+ SVNL+NTMLD+  K
Sbjct: 241 FNSMLSSDVEPNEVTMIALLSASSQKGDSVMGRVLHEHIRRKGITCSVNLLNTMLDMYVK 300

BLAST of Carg22174 vs. ExPASy Swiss-Prot
Match: Q9SJZ3 (Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E28 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 9.7e-44
Identity = 95/261 (36.40%), Postives = 148/261 (56.70%), Query Frame = 0

Query: 43  WNSLTNVFITNPTLLILQSCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDI 102
           WNS  +  + NP L +L+ C  + HLKQIQA M   GL+   F +SR++AFCALS++  +
Sbjct: 44  WNSTHSFVLHNPLLSLLEKCKLLLHLKQIQAQMIINGLILDPFASSRLIAFCALSESRYL 103

Query: 103 HYAHLVFAQIENPNCFLWNTMIKGYCRAKCPSMGFLFFRQMIRN---RVELDCGSFVSAL 162
            Y+  +   IENPN F WN  I+G+  ++ P   FL ++QM+R+       D  ++    
Sbjct: 104 DYSVKILKGIENPNIFSWNVTIRGFSESENPKESFLLYKQMLRHGCCESRPDHFTYPVLF 163

Query: 163 KVCGQFSGKAIRMAVHSVVWKLGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVV 222
           KVC      ++   +   V KL       V N  I  +   G +  AR+VFDES V+D+V
Sbjct: 164 KVCADLRLSSLGHMILGHVLKLRLELVSHVHNASIHMFASCGDMENARKVFDESPVRDLV 223

Query: 223 TWTTMINGYTTNNCLDEAIMLFDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELI 282
           +W  +INGY      ++AI ++ +M S  V+P++VTMI L+S+CS  GD   G+  +E +
Sbjct: 224 SWNCLINGYKKIGEAEKAIYVYKLMESEGVKPDDVTMIGLVSSCSMLGDLNRGKEFYEYV 283

Query: 283 KRKNISSSVNLVNTMLDISSK 301
           K   +  ++ LVN ++D+ SK
Sbjct: 284 KENGLRMTIPLVNALMDMFSK 304

BLAST of Carg22174 vs. ExPASy Swiss-Prot
Match: O82380 (Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H33 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.6e-33
Identity = 79/244 (32.38%), Postives = 129/244 (52.87%), Query Frame = 0

Query: 58  ILQSCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNC 117
           +++ C S+  LKQ   HM  TG  +  + AS++ A  ALS    + YA  VF +I  PN 
Sbjct: 36  LIERCVSLRQLKQTHGHMIRTGTFSDPYSASKLFAMAALSSFASLEYARKVFDEIPKPNS 95

Query: 118 FLWNTMIKGYCRAKCPSMGFLFFRQMI-RNRVELDCGSFVSALKVCGQFSGKAIRMAVHS 177
           F WNT+I+ Y     P +    F  M+  ++   +  +F   +K   + S  ++  ++H 
Sbjct: 96  FAWNTLIRAYASGPDPVLSIWAFLDMVSESQCYPNKYTFPFLIKAAAEVSSLSLGQSLHG 155

Query: 178 VVWKLGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDE 237
           +  K   GS++ V N LI  Y   G +  A +VF     KDVV+W +MING+      D+
Sbjct: 156 MAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKEKDVVSWNSMINGFVQKGSPDK 215

Query: 238 AIMLFDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLD 297
           A+ LF  M S +V+ + VTM+ +LSAC++  +   GR +   I+   ++ ++ L N MLD
Sbjct: 216 ALELFKKMESEDVKASHVTMVGVLSACAKIRNLEFGRQVCSYIEENRVNVNLTLANAMLD 275

Query: 298 ISSK 301
           + +K
Sbjct: 276 MYTK 279

BLAST of Carg22174 vs. ExPASy Swiss-Prot
Match: Q9FG16 (Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H88 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 7.7e-33
Identity = 84/288 (29.17%), Postives = 146/288 (50.69%), Query Frame = 0

Query: 46  LTNVFITNPTLLILQSCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGD---- 105
           L  +   +P L +LQSCSS   LK I   +  T L++ +F ASR++A C      +    
Sbjct: 6   LNTLRFKHPKLALLQSCSSFSDLKIIHGFLLRTHLISDVFVASRLLALCVDDSTFNKPTN 65

Query: 106 -IHYAHLVFAQIENPNCFLWNTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALK 165
            + YA+ +F+QI+NPN F++N +I+ +     PS  F F+ QM+++R+  D  +F   +K
Sbjct: 66  LLGYAYGIFSQIQNPNLFVFNLLIRCFSTGAEPSKAFGFYTQMLKSRIWPDNITFPFLIK 125

Query: 166 VCGQFSGKAIRMAVHSVVWKLGFGSELLVQNGLIQYYVETGCI----------GF----- 225
              +     +    HS + + GF +++ V+N L+  Y   G I          GF     
Sbjct: 126 ASSEMECVLVGEQTHSQIVRFGFQNDVYVENSLVHMYANCGFIAAAGRIFGQMGFRDVVS 185

Query: 226 ----------------ARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIMLFDMMSSSNV 285
                           AR++FDE   +++ TW+ MINGY  NNC ++AI LF+ M    V
Sbjct: 186 WTSMVAGYCKCGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCFEKAIDLFEFMKREGV 245

Query: 286 EPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDI 298
             NE  M++++S+C+  G    G   +E + + +++ ++ L   ++D+
Sbjct: 246 VANETVMVSVISSCAHLGALEFGERAYEYVVKSHMTVNLILGTALVDM 293

BLAST of Carg22174 vs. ExPASy Swiss-Prot
Match: Q9LN01 (Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H12 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.7e-32
Identity = 92/302 (30.46%), Postives = 143/302 (47.35%), Query Frame = 0

Query: 31  PLSSSAAAGKPKWNSLTNVFITNPTLLILQSCSSMFHLKQIQAHMTCTGLMNQIFPASRV 90
           P     ++  P ++S+ N    +P+L +L +C ++  L+ I A M   GL N  +  S++
Sbjct: 16  PFHFLPSSSDPPYDSIRN----HPSLSLLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKL 75

Query: 91  VAFCALSDAGD-IHYAHLVFAQIENPNCFLWNTMIKGYCRAKCPSMGFLFFRQMIRNRVE 150
           + FC LS   + + YA  VF  I+ PN  +WNTM +G+  +  P      +  MI   + 
Sbjct: 76  IEFCILSPHFEGLPYAISVFKTIQEPNLLIWNTMFRGHALSSDPVSALKLYVCMISLGLL 135

Query: 151 LDCGSFVSALKVCGQFSGKAIRMAVHSVVWKLGFGSELLVQNGLIQYYVETGCIGFARQV 210
            +  +F   LK C +         +H  V KLG   +L V   LI  YV+ G +  A +V
Sbjct: 136 PNSYTFPFVLKSCAKSKAFKEGQQIHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKV 195

Query: 211 FDES-------------------------------SVKDVVTWTTMINGYTTNNCLDEAI 270
           FD+S                                VKDVV+W  MI+GY       EA+
Sbjct: 196 FDKSPHRDVVSYTALIKGYASRGYIENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEAL 255

Query: 271 MLFDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDIS 301
            LF  M  +NV P+E TM+ ++SAC+Q G   +GR +H  I      S++ +VN ++D+ 
Sbjct: 256 ELFKDMMKTNVRPDESTMVTVVSACAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLY 313

BLAST of Carg22174 vs. ExPASy Swiss-Prot
Match: Q9LUJ2 (Pentatricopeptide repeat-containing protein At3g22690 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H56 PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 5.0e-32
Identity = 82/279 (29.39%), Postives = 146/279 (52.33%), Query Frame = 0

Query: 25  ILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQSCSSMFHLKQIQAHMTCTGLMNQI 84
           +LH    + ++    KP  + L     T  T   L++C ++  LK     +T  GL N +
Sbjct: 7   VLHLSPMVLATTTTTKP--SLLNQSKCTKATPSSLKNCKTIDELKMFHRSLTKQGLDNDV 66

Query: 85  FPASRVVA-FCALSDAGDIHYAHLVFAQIEN-PNCFLWNTMIKGYCRAKCPSMGFLFFRQ 144
              +++VA  C L     + +A  VF   E+   CF++N++I+GY  +   +   L F +
Sbjct: 67  STITKLVARSCELGTRESLSFAKEVFENSESYGTCFMYNSLIRGYASSGLCNEAILLFLR 126

Query: 145 MIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWKLGFGSELLVQNGLIQYYVETGC 204
           M+ + +  D  +F   L  C +   K   + +H ++ K+G+  +L VQN L+ +Y E G 
Sbjct: 127 MMNSGISPDKYTFPFGLSACAKSRAKGNGIQIHGLIVKMGYAKDLFVQNSLVHFYAECGE 186

Query: 205 IGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAI-MLFDMMSSSNVEPNEVTMIALLS 264
           +  AR+VFDE S ++VV+WT+MI GY   +   +A+ + F M+    V PN VTM+ ++S
Sbjct: 187 LDSARKVFDEMSERNVVSWTSMICGYARRDFAKDAVDLFFRMVRDEEVTPNSVTMVCVIS 246

Query: 265 ACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 301
           AC++  D   G  ++  I+   I  +  +V+ ++D+  K
Sbjct: 247 ACAKLEDLETGEKVYAFIRNSGIEVNDLMVSALVDMYMK 283

BLAST of Carg22174 vs. ExPASy TrEMBL
Match: A0A6J1HB35 (pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111461192 PE=4 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.1e-166
Identity = 293/300 (97.67%), Postives = 297/300 (99.00%), Query Frame = 0

Query: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60
           MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ
Sbjct: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60

Query: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120
           SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW
Sbjct: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120

Query: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWK 180
           NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQF+GKA+RMAVHSVVWK
Sbjct: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFAGKAVRMAVHSVVWK 180

Query: 181 LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240
           LGFGSELLVQNGLIQYYVE GCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML
Sbjct: 181 LGFGSELLVQNGLIQYYVEIGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240

Query: 241 FDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 300
           FD+MSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLD+  K
Sbjct: 241 FDLMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDMYVK 300

BLAST of Carg22174 vs. ExPASy TrEMBL
Match: A0A6J1KS94 (pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111497776 PE=4 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 3.5e-161
Identity = 286/300 (95.33%), Postives = 291/300 (97.00%), Query Frame = 0

Query: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60
           MKPLFSSVQN SRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ
Sbjct: 1   MKPLFSSVQNSSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60

Query: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120
           SCSSMFHLKQIQAHM CTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW
Sbjct: 61  SCSSMFHLKQIQAHMICTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120

Query: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWK 180
           NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALK CG F+GKAIRMAVHSVVWK
Sbjct: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKACGHFAGKAIRMAVHSVVWK 180

Query: 181 LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240
           LGFGS LLVQNGLIQ+YVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNN LDEA+ML
Sbjct: 181 LGFGSGLLVQNGLIQFYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNYLDEAVML 240

Query: 241 FDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 300
           FD+M SSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLD+  K
Sbjct: 241 FDLMLSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDMYVK 300

BLAST of Carg22174 vs. ExPASy TrEMBL
Match: A0A1S3BSJ4 (LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC103492719 PE=4 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 8.9e-133
Identity = 234/300 (78.00%), Postives = 263/300 (87.67%), Query Frame = 0

Query: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60
           MKP FSS+QNPS  SIFP  L  KILHSFRPLS+S++AGKPKWNSL+NVFI+NPTLLILQ
Sbjct: 1   MKPFFSSIQNPSHISIFPLNLAVKILHSFRPLSTSSSAGKPKWNSLSNVFISNPTLLILQ 60

Query: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120
           SCSSMF LKQIQAHMTCTGLM+QIFPASR++AFCALSD+GDIHYAHL+F Q E+PNCF W
Sbjct: 61  SCSSMFQLKQIQAHMTCTGLMSQIFPASRLLAFCALSDSGDIHYAHLIFDQTEHPNCFFW 120

Query: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWK 180
           NTMIKGYC+A  PS+GF FFRQMIRNR E DCGSFV ALK CGQF+ K + MAVHSV+WK
Sbjct: 121 NTMIKGYCQANYPSLGFSFFRQMIRNRAEFDCGSFVFALKACGQFAEKIVGMAVHSVIWK 180

Query: 181 LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240
           LGF SELLV+NGLIQ YVETGC+GFARQVFDESS+KDVVTWTTMINGY  +N LDEAI L
Sbjct: 181 LGFDSELLVRNGLIQNYVETGCLGFARQVFDESSIKDVVTWTTMINGYARSNWLDEAIAL 240

Query: 241 FDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 300
           F+ M SS+VEPNEVTMIALLSACSQKGD VMG++LHE I+ K I+ SVNL+N MLD+  K
Sbjct: 241 FNSMLSSDVEPNEVTMIALLSACSQKGDSVMGKTLHEHIRGKGITCSVNLLNAMLDMYVK 300

BLAST of Carg22174 vs. ExPASy TrEMBL
Match: A0A5A7USA2 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G002100 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.2e-132
Identity = 234/300 (78.00%), Postives = 262/300 (87.33%), Query Frame = 0

Query: 1   MKPLFSSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQ 60
           MKP FSS+QNPS  SIFP  L  KILHSFRPLS+S++AGKPKWNSL+NVFI+NPTLLILQ
Sbjct: 1   MKPFFSSIQNPSHISIFPLNLAVKILHSFRPLSTSSSAGKPKWNSLSNVFISNPTLLILQ 60

Query: 61  SCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFLW 120
           SCSSMF LKQIQAHMTCTGLM+QIFPASR++AFCALSD+GDIHYAHL+F Q E+PNCF W
Sbjct: 61  SCSSMFQLKQIQAHMTCTGLMSQIFPASRLLAFCALSDSGDIHYAHLIFDQTEHPNCFFW 120

Query: 121 NTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWK 180
           NTMIKGYC+A  PSMGF FFRQMIRNR E DCGSFV ALK CGQF+ K + MAVHSV+WK
Sbjct: 121 NTMIKGYCQANYPSMGFSFFRQMIRNRAEFDCGSFVFALKACGQFAEKIVGMAVHSVIWK 180

Query: 181 LGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIML 240
           LGF SEL V+NGLIQ YVETGC+GFARQVFDESS+KDVVTWTTMINGY  +N LDEAI L
Sbjct: 181 LGFDSELFVRNGLIQNYVETGCLGFARQVFDESSIKDVVTWTTMINGYAKSNWLDEAIAL 240

Query: 241 FDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 300
           F+ M SS+VEPNEVTMIALLSACSQKGD VMG++LHE I+ K I+ SVNL+N MLD+  K
Sbjct: 241 FNSMLSSDVEPNEVTMIALLSACSQKGDSVMGKTLHEHIRGKGITCSVNLLNAMLDMYVK 300

BLAST of Carg22174 vs. ExPASy TrEMBL
Match: A0A6J1D7Q3 (pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111018407 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.1e-130
Identity = 239/301 (79.40%), Postives = 259/301 (86.05%), Query Frame = 0

Query: 1   MKPLF-SSVQNPSRFSIFPFKLTFKILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLIL 60
           MKPLF SS+QNP++F IFP  L  +I H  R LSSS +AGKPKWNSL NVFI+NPTLLIL
Sbjct: 1   MKPLFSSSIQNPTQFLIFPLNLRLQIPHFLRSLSSSTSAGKPKWNSLANVFISNPTLLIL 60

Query: 61  QSCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNCFL 120
           QSCSSMF LKQI AHMTCTGLMNQIFPASRVVAFCALSD+GD+ YAH VFA+IE PNCFL
Sbjct: 61  QSCSSMFQLKQIHAHMTCTGLMNQIFPASRVVAFCALSDSGDMRYAHFVFARIEKPNCFL 120

Query: 121 WNTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVW 180
           WNTMIKGYC A   SMGF FFRQMIRNRVELDCGSFV ALK CGQF+G+   MAVH V+W
Sbjct: 121 WNTMIKGYCSAGNRSMGFSFFRQMIRNRVELDCGSFVFALKACGQFAGEIEGMAVHCVIW 180

Query: 181 KLGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIM 240
           KLGF SELLV+NGLIQYYVETG + FARQVFDESSVKDVVTWTTMINGYTT+NCLDEAI 
Sbjct: 181 KLGFDSELLVRNGLIQYYVETGYLFFARQVFDESSVKDVVTWTTMINGYTTHNCLDEAIT 240

Query: 241 LFDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISS 300
           LFD M SS+VEPNEVTMI LLSACSQKGD +MGR+LHE I RKNI  SVNL+NTMLD+  
Sbjct: 241 LFDSMLSSDVEPNEVTMITLLSACSQKGDSIMGRTLHEYIMRKNIRCSVNLLNTMLDMYV 300

BLAST of Carg22174 vs. TAIR 10
Match: AT2G22410.1 (SLOW GROWTH 1 )

HSP 1 Score: 178.7 bits (452), Expect = 6.9e-45
Identity = 95/261 (36.40%), Postives = 148/261 (56.70%), Query Frame = 0

Query: 43  WNSLTNVFITNPTLLILQSCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDI 102
           WNS  +  + NP L +L+ C  + HLKQIQA M   GL+   F +SR++AFCALS++  +
Sbjct: 44  WNSTHSFVLHNPLLSLLEKCKLLLHLKQIQAQMIINGLILDPFASSRLIAFCALSESRYL 103

Query: 103 HYAHLVFAQIENPNCFLWNTMIKGYCRAKCPSMGFLFFRQMIRN---RVELDCGSFVSAL 162
            Y+  +   IENPN F WN  I+G+  ++ P   FL ++QM+R+       D  ++    
Sbjct: 104 DYSVKILKGIENPNIFSWNVTIRGFSESENPKESFLLYKQMLRHGCCESRPDHFTYPVLF 163

Query: 163 KVCGQFSGKAIRMAVHSVVWKLGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVV 222
           KVC      ++   +   V KL       V N  I  +   G +  AR+VFDES V+D+V
Sbjct: 164 KVCADLRLSSLGHMILGHVLKLRLELVSHVHNASIHMFASCGDMENARKVFDESPVRDLV 223

Query: 223 TWTTMINGYTTNNCLDEAIMLFDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELI 282
           +W  +INGY      ++AI ++ +M S  V+P++VTMI L+S+CS  GD   G+  +E +
Sbjct: 224 SWNCLINGYKKIGEAEKAIYVYKLMESEGVKPDDVTMIGLVSSCSMLGDLNRGKEFYEYV 283

Query: 283 KRKNISSSVNLVNTMLDISSK 301
           K   +  ++ LVN ++D+ SK
Sbjct: 284 KENGLRMTIPLVNALMDMFSK 304

BLAST of Carg22174 vs. TAIR 10
Match: AT2G29760.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 144.8 bits (364), Expect = 1.1e-34
Identity = 79/244 (32.38%), Postives = 129/244 (52.87%), Query Frame = 0

Query: 58  ILQSCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGDIHYAHLVFAQIENPNC 117
           +++ C S+  LKQ   HM  TG  +  + AS++ A  ALS    + YA  VF +I  PN 
Sbjct: 36  LIERCVSLRQLKQTHGHMIRTGTFSDPYSASKLFAMAALSSFASLEYARKVFDEIPKPNS 95

Query: 118 FLWNTMIKGYCRAKCPSMGFLFFRQMI-RNRVELDCGSFVSALKVCGQFSGKAIRMAVHS 177
           F WNT+I+ Y     P +    F  M+  ++   +  +F   +K   + S  ++  ++H 
Sbjct: 96  FAWNTLIRAYASGPDPVLSIWAFLDMVSESQCYPNKYTFPFLIKAAAEVSSLSLGQSLHG 155

Query: 178 VVWKLGFGSELLVQNGLIQYYVETGCIGFARQVFDESSVKDVVTWTTMINGYTTNNCLDE 237
           +  K   GS++ V N LI  Y   G +  A +VF     KDVV+W +MING+      D+
Sbjct: 156 MAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKEKDVVSWNSMINGFVQKGSPDK 215

Query: 238 AIMLFDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLD 297
           A+ LF  M S +V+ + VTM+ +LSAC++  +   GR +   I+   ++ ++ L N MLD
Sbjct: 216 ALELFKKMESEDVKASHVTMVGVLSACAKIRNLEFGRQVCSYIEENRVNVNLTLANAMLD 275

Query: 298 ISSK 301
           + +K
Sbjct: 276 MYTK 279

BLAST of Carg22174 vs. TAIR 10
Match: AT5G06540.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 5.5e-34
Identity = 84/288 (29.17%), Postives = 146/288 (50.69%), Query Frame = 0

Query: 46  LTNVFITNPTLLILQSCSSMFHLKQIQAHMTCTGLMNQIFPASRVVAFCALSDAGD---- 105
           L  +   +P L +LQSCSS   LK I   +  T L++ +F ASR++A C      +    
Sbjct: 6   LNTLRFKHPKLALLQSCSSFSDLKIIHGFLLRTHLISDVFVASRLLALCVDDSTFNKPTN 65

Query: 106 -IHYAHLVFAQIENPNCFLWNTMIKGYCRAKCPSMGFLFFRQMIRNRVELDCGSFVSALK 165
            + YA+ +F+QI+NPN F++N +I+ +     PS  F F+ QM+++R+  D  +F   +K
Sbjct: 66  LLGYAYGIFSQIQNPNLFVFNLLIRCFSTGAEPSKAFGFYTQMLKSRIWPDNITFPFLIK 125

Query: 166 VCGQFSGKAIRMAVHSVVWKLGFGSELLVQNGLIQYYVETGCI----------GF----- 225
              +     +    HS + + GF +++ V+N L+  Y   G I          GF     
Sbjct: 126 ASSEMECVLVGEQTHSQIVRFGFQNDVYVENSLVHMYANCGFIAAAGRIFGQMGFRDVVS 185

Query: 226 ----------------ARQVFDESSVKDVVTWTTMINGYTTNNCLDEAIMLFDMMSSSNV 285
                           AR++FDE   +++ TW+ MINGY  NNC ++AI LF+ M    V
Sbjct: 186 WTSMVAGYCKCGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCFEKAIDLFEFMKREGV 245

Query: 286 EPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDI 298
             NE  M++++S+C+  G    G   +E + + +++ ++ L   ++D+
Sbjct: 246 VANETVMVSVISSCAHLGALEFGERAYEYVVKSHMTVNLILGTALVDM 293

BLAST of Carg22174 vs. TAIR 10
Match: AT1G08070.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.2e-33
Identity = 92/302 (30.46%), Postives = 143/302 (47.35%), Query Frame = 0

Query: 31  PLSSSAAAGKPKWNSLTNVFITNPTLLILQSCSSMFHLKQIQAHMTCTGLMNQIFPASRV 90
           P     ++  P ++S+ N    +P+L +L +C ++  L+ I A M   GL N  +  S++
Sbjct: 16  PFHFLPSSSDPPYDSIRN----HPSLSLLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKL 75

Query: 91  VAFCALSDAGD-IHYAHLVFAQIENPNCFLWNTMIKGYCRAKCPSMGFLFFRQMIRNRVE 150
           + FC LS   + + YA  VF  I+ PN  +WNTM +G+  +  P      +  MI   + 
Sbjct: 76  IEFCILSPHFEGLPYAISVFKTIQEPNLLIWNTMFRGHALSSDPVSALKLYVCMISLGLL 135

Query: 151 LDCGSFVSALKVCGQFSGKAIRMAVHSVVWKLGFGSELLVQNGLIQYYVETGCIGFARQV 210
            +  +F   LK C +         +H  V KLG   +L V   LI  YV+ G +  A +V
Sbjct: 136 PNSYTFPFVLKSCAKSKAFKEGQQIHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKV 195

Query: 211 FDES-------------------------------SVKDVVTWTTMINGYTTNNCLDEAI 270
           FD+S                                VKDVV+W  MI+GY       EA+
Sbjct: 196 FDKSPHRDVVSYTALIKGYASRGYIENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEAL 255

Query: 271 MLFDMMSSSNVEPNEVTMIALLSACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDIS 301
            LF  M  +NV P+E TM+ ++SAC+Q G   +GR +H  I      S++ +VN ++D+ 
Sbjct: 256 ELFKDMMKTNVRPDESTMVTVVSACAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLY 313

BLAST of Carg22174 vs. TAIR 10
Match: AT3G22690.1 (CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1685 (InterPro:IPR012881), Pentatricopeptide repeat (InterPro:IPR002885); BEST Arabidopsis thaliana protein match is: Tetratricopeptide repeat (TPR)-like superfamily protein (TAIR:AT2G29760.1); Has 49784 Blast hits to 14716 proteins in 280 species: Archae - 2; Bacteria - 10; Metazoa - 107; Fungi - 167; Plants - 48594; Viruses - 0; Other Eukaryotes - 904 (source: NCBI BLink). )

HSP 1 Score: 139.8 bits (351), Expect = 3.5e-33
Identity = 82/279 (29.39%), Postives = 146/279 (52.33%), Query Frame = 0

Query: 25  ILHSFRPLSSSAAAGKPKWNSLTNVFITNPTLLILQSCSSMFHLKQIQAHMTCTGLMNQI 84
           +LH    + ++    KP  + L     T  T   L++C ++  LK     +T  GL N +
Sbjct: 7   VLHLSPMVLATTTTTKP--SLLNQSKCTKATPSSLKNCKTIDELKMFHRSLTKQGLDNDV 66

Query: 85  FPASRVVA-FCALSDAGDIHYAHLVFAQIEN-PNCFLWNTMIKGYCRAKCPSMGFLFFRQ 144
              +++VA  C L     + +A  VF   E+   CF++N++I+GY  +   +   L F +
Sbjct: 67  STITKLVARSCELGTRESLSFAKEVFENSESYGTCFMYNSLIRGYASSGLCNEAILLFLR 126

Query: 145 MIRNRVELDCGSFVSALKVCGQFSGKAIRMAVHSVVWKLGFGSELLVQNGLIQYYVETGC 204
           M+ + +  D  +F   L  C +   K   + +H ++ K+G+  +L VQN L+ +Y E G 
Sbjct: 127 MMNSGISPDKYTFPFGLSACAKSRAKGNGIQIHGLIVKMGYAKDLFVQNSLVHFYAECGE 186

Query: 205 IGFARQVFDESSVKDVVTWTTMINGYTTNNCLDEAI-MLFDMMSSSNVEPNEVTMIALLS 264
           +  AR+VFDE S ++VV+WT+MI GY   +   +A+ + F M+    V PN VTM+ ++S
Sbjct: 187 LDSARKVFDEMSERNVVSWTSMICGYARRDFAKDAVDLFFRMVRDEEVTPNSVTMVCVIS 246

Query: 265 ACSQKGDYVMGRSLHELIKRKNISSSVNLVNTMLDISSK 301
           AC++  D   G  ++  I+   I  +  +V+ ++D+  K
Sbjct: 247 ACAKLEDLETGEKVYAFIRNSGIEVNDLMVSALVDMYMK 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025550.12.5e-169100.00Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_022960464.14.3e-16697.67pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like [Cucur... [more]
XP_023513845.11.8e-16496.33pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like [Cucur... [more]
XP_023004481.17.1e-16195.33pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like [Cucur... [more]
XP_038898970.12.6e-13481.00pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like [Benin... [more]
Match NameE-valueIdentityDescription
Q9SJZ39.7e-4436.40Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidop... [more]
O823801.6e-3332.38Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidop... [more]
Q9FG167.7e-3329.17Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX... [more]
Q9LN011.7e-3230.46Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidop... [more]
Q9LUJ25.0e-3229.39Pentatricopeptide repeat-containing protein At3g22690 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A6J1HB352.1e-16697.67pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like OS=Cuc... [more]
A0A6J1KS943.5e-16195.33pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like OS=Cuc... [more]
A0A1S3BSJ48.9e-13378.00LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At2g22410, mito... [more]
A0A5A7USA21.2e-13278.00Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1D7Q31.1e-13079.40pentatricopeptide repeat-containing protein At2g22410, mitochondrial-like OS=Mom... [more]
Match NameE-valueIdentityDescription
AT2G22410.16.9e-4536.40SLOW GROWTH 1 [more]
AT2G29760.11.1e-3432.38Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G06540.15.5e-3429.17Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G08070.11.2e-3330.46Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G22690.13.5e-3329.39CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1685 (InterPro:IPR012... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 59..176
e-value: 2.4E-14
score: 55.1
coord: 183..300
e-value: 1.4E-21
score: 78.6
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 119..147
e-value: 1.5E-6
score: 28.0
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 119..152
e-value: 6.6E-8
score: 30.3
coord: 219..253
e-value: 2.1E-9
score: 35.0
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 216..263
e-value: 1.9E-13
score: 50.4
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 116..150
score: 10.281757
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 217..251
score: 12.156139
NoneNo IPR availablePANTHERPTHR47929:SF1SUBFAMILY NOT NAMEDcoord: 186..300
coord: 97..267
NoneNo IPR availablePANTHERPTHR47929FAMILY NOT NAMEDcoord: 186..300
coord: 97..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg22174-RACarg22174-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding