Carg22161 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22161
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	E3 ubiquitin-protein ligase MIEL1
Location	Carg_Chr08: 2237476 .. 2243577 (-)
RNA-Seq Expression	Carg22161
Synteny	Carg22161

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCGATTAAAGCCCTACTTTCCATTGCTCTTATCGCTCTTCTCTTGTTCTTCGAATCCCTTCCAACTTCCGCCGCCGTTCACCGCGGGGCTTCCACTTCTCTTGCTGGAATACTTCGCCGGAACATCGTACAGACGACGGCGGATCATCATCAAGTTGCAGAGAAGAAGTTCAACGTTCATATCAAAAGGAGGATGCGGATGCGACCTGTGCTAATTGGCCGCCCTAAGACATCGAAGGCGAATCGGAGCCGAGAAGCGTTCTGTGGCGTTGGATCTATGCTGTGTATCTTCGTTCTTTTGGGATTGTTCTTGTGAATCATCCGTTTCTGTAAAACTTGGATTCATTTTTTTCATTTCATTTTATTTATATTTATACGATAAAAATACAGGTTTTTTTTTTTTTTTTTTAAATTATTATTTTATTTTAAATTGGTTTATTCTCTTTTTCATTTTTTTTTTTTGTAATTAAGCCTCGAGATTATTTGTGAAATTATAAAAATACATTTATAGTGATTGATAATATCAATTCTAAACACATTTTAATAAATAAAACTATGCAAAAAATTTCAATAAAAATTCCTCTCCATTCGAGGGAGTCGCATGGAAAAAACCGACACATCGATTTAACGTGCACGTGCACATGCACATGCACCTAATTCTTCCCAAACTCGAGCTAAATGGCTTCCACCATAGTTCTTGTTCTTTTCTCGACGTCTCTATTCTTCTTGGTCGGAATATTCTTCGGTAGATGTAACGTCCTAGATCCACCGCTAGGAGATATTGTCCTCTTTGGGCTTTCCCTTTCGGGCTTCTCCTCATTGTTCTCCTCCCCAACCAGTGTAGGACATCACAATCCACCCCCCTTCGGGGCCCAACGTCCTCGCTGGCACTCTTTCCTTCCTCCAATTGATGTGGGACCGCCCCCAAATCCATTCCCCTTTGGGCCCAGCGTCCTTACTAGCACACCGCCTCGTGTCTACCCCCCTTCGGGGAACAGCAAGAAGGCTGACACATCGTCCAGTGTCTAGCTCTGATACCATTTGTAACGTCCCAGATCCACCGCTAGCAGATATTGTCATTTTTGGGCTTTCCCTTTCGGGCTTCCCCTCAAGGCTTTAAAACGTGTCTGCTAGGGGAAGGTTTGCACACCCCTATAAATAGTGGTTTGTTCTCCTCCCCAACCAATATGGGATATCACAGTAGATGATCATCATCAAGTTGCTAAAATCAATGGTCGATACCTTTTCGAACGAAAGATCATATCACAGCGGGCTTGATTATCTGTAGCGAGAGCTGAAGCTCATCAACGGCGAATCGGAGCTAAACGTCATCGTTTGGGATGATGTTTGTGTTGTGTCATTGTTTTGTTCTTGGTTTGCTCGTGTGAGAGTTTGATTATTCACTTCTCGAATATTTTGAGTTGTACTTATTGGTATGGATTTAGGATTTTGTATCCGGCTTTGTGAGGTCAATTAATTTAAATTATAAATTTCATTTAATTGTTTATTAATTAAATTTAAATTTAAATATTTTGTAGCATGATTAAAATATCTTATTTCGTATCACGTATCACTAATAAGAGATTTCGGGTTCTCAATAGGTTCTCGTGCGCTTGTCCTGTCCAACGAGAAGCTTCCGGAAGCACTTCGACGTATCTATTTAACCTACGAGGTTAATGTTATTTTCCATAAAATAATATTTTATTTACTCCCATGAGATTATAAAATAAAATAAAAAGGGAAAATTCCTTAATAAAACAATTTCAAGCTTTGTAACTATTGTCGAACCAAACCAGAGAGAACGAACAGGGTTGGTCTCCAATGGAACAAGACGACTGCCCTGTGCCCCAATCTGCCGGGAGAGAAGATTTCGGGAAATTGCAGCACGGGTACCGATTTCTTTCCTTCATGAGGCGGCCTTGGCCGTCTTCTTCTTGTTCGTCCTCGTTTTCGTCATCAGTAACTTCTTTTCGATTTGAATTAGATGCGAGCATTATAGGAGACGATGCAGAATCCGAGCCCCGTGCTGCGACGGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGGTATTTCTCTCTCCTTTCTGCGTTGTGTGTGCGTGTGAGAGAGGCGTGTAATAAGGAATTGTGTATATGTTGTTCAACTGGAGCTTTCAGCTGAATTTATGAATTTTCATTGGTTTTAGAGCTCGCTGAGCAATCCGAAGGACCGCCACGAACTTGTTCGACAGGACGTTAGACAAGTCTGTTTGTACGCTGTTCTATTGATTTTTTTTTTTTTTTTCCGGTTAAATTGTTGATTGTAACACATCGTGTAAAGGGCGTCATAATAGGATTTGATTGATTTTGTTGGAATTGATGCTTTAGGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGTTTGGGGGTTTCTTTCCTTTTCTTTTTGGATAATTTCATTCTTTGTGTTTATATGTTCTTGTTTATGTATTAAACGTTTGAATTTGGTGCATTTTAGGCCGTCAGGGTTTGTCGCAACTGTAATGTTAATATGGGAGAATACTTTTGCGATATCTGCAAATTCTACGATGATGATGTAGGAAAAGTTTCCATCAATCCGTTTCCCTTTATTTAGGAACTGATTTTGAATTTTGAAGTGTGGAACGGAATGATCTATTTATCCTAGATGCTAATAGTTTCTGTTGTGAATGCAGACCAAAAAGAAGCAGTTTCATTGTGATGCTTGTGGTATCTGTAGGTAAGGGTGCAATTTTCCGGTTCTAATTCTAATGTAAACTCAGCTTTCTGTGTTAAGTTTCTAAATAAGTATTCTTTGTAATATGATTTTCAGAGTTGGTGGTCGTGAAAACTTCTTTCACTGTGAAAAGTGTGGTATGTTATTCAACTCTCTTGATGATTCGTTATAACTTTGTCTTGTGTTGTAACTTCTTTTACTGCAAGCAATTACTATAAGTTATTTGCTTATGATACCTTCAGGATTAGTGCAACTAATTCACTGCCTATTAAGGTCTTTTTTTCCCTTTGTTTTTGGAAAAGTTGTTTGATGAGACCTTTCACAATGCAACCGGTAGGTTTGTGTGAGATCTCTCATCGGTTGGAGAGGGGAACAAAGTATTCTTTATAAAGGTGTAAAAACCTCTCCCTAGCAGACGCGTTTTAAAAGGGCAAGCCGAAAGAAGATAATATCTGCTAGTGGTGGGCTTGAGCTATTACAGTTTGCCCTTTTTGATGCGTGAGAGAGAATTTTTTTTAAAGACAATATTTAATGACACATTTAGAGCTCATCAAACCATTATAACCAACTAAAAGCTAACCTCACCACTGAACAAGTTTTGGAATTGCAAATTGGAAAGATAACTACACATCTATCTCCTTTAGGGCACAAGTGAAATGAATTAGCTTTAGGTGTCGGTTGCTCTGGTGTAGATCTGCTGACGTCAGTGAGCTGAGATCATTCTGATAGAACCTCATGCAAGCAAGCCATTGATCCTCAGCCAATCAGAAAGGATTGAACTAGCTTGTACTTGGTAACTTCAAAAAGAAGCTAGTCATAAGTTCCGTTATCAAATGAAACCATGTTGTTCATCTTCTCCTTTTGCTTACCCTTGCCCCATTTCTTCTTCTTCTGTTGCTTCCTCCTTGTTCGGCGCCACAAGATCTGATTTGAGCGTTTGAGTGAAGGAGTTGCAAAGGTTTTGGCTTGAACAAAGACGTGCGAGAGAGAGATTATAAAAGTATTTCATTTTTGTATCCAATGACTTATTTTTGGATCTACATCTTACAGTTATTAAGAGGTGGCCTCATTGAATAATTTGTTCTATTTTTGTGTTTGTGAGGTGGGTCGATAGGGAGATTAACATTTTGTTATAAGTACAGGATCGTGCTATTCCACCCCGCTGCGCGATAACCACATGTGTGTTGAGAACTCTATGAAAAGTTTTTGTCCAATCTGTTATGAGGTTTGTGCTTTTAGATTGCGTGTTTTCCCAGGAAGGTACAATCTTAACTGTTCTCTTCTAACAAATTTATTGATGCTTGCAGTTTCTTTTTGACTCAATTAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAGATGGAATCACAGAATCAGTACTTGAATTCTTAACTCAACATCCAGTTTCTGAATATACTAAAATATTTCTAAAAACTTTGCAAATACAAAATACAACTCTATTTTCTGTTATATCCATCGTCTTTATTCTCAAACCAAACAGGTTCTAAGCTTCCTGTTTTGAATTACATTAAGATTTCCGGTTCTCTTATTCTAATGTAGTAATATTTGCCTCTCAGATACCGTTGTCCTATCTGCTCCAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGGTGTGTAACACTTAGTTCAGTCATGATAAAAATGAAAAGTAGAAGGAAATTAGACAACTGTCGTTTTAATTCTTAGAGAGGTTTCCCCAGTTAGTGAACACGATAGAACAGGTGGCTTCACATAAATTTTAGCAACCTTAGAGATTAGAATCTGTATCTTATCATTGTACATTGTACCCCCACTCTTCAACCTTCTGACTGATTTTTTCATTTTTTTTTCTTTTTGTCGTGATGTATGATTACATGTATTGGTCTGTTCGTTAGAAAGTTGGTGGTTGCAGACTTATTATCTTAATAGAAATCTGACTGGAGTGATTGTCTGTTGGAAAGAAAATGAGTGTAGATTCAAGGTTTTAGTTATGCATTTCAGTTGAATTGCGAGAGAGGGATTGAGAGGCAAGATGAAAGAAGCAGTCCATGAGAGCTTAATACTTTCTGTTTCGGTTTGACTTGCTCTGTTTACGACTTTAAATTTTGTCCTTTTCCATTTGCCCATTACCCATTACCCAGTTGATATTTATGGTAAAATCGATCCTGTTGAATACTAAACTTTTCGGGTCACCGAGTGGTGGTTCTTTATATTTGTAAAGACATGTTTCTTCTGTCATATTTCAGTGTTGATATTCCGAAGTTTCTTTGGTTTCTTAATTGTGTTTACTGTACCGTAGACCTTTGGCATTCCATATTATCTTAAATTTTAAATTCTATTCTTATCTTTTTCTAGATTGACTGCACACCGATGCCTGAGGAATACAGCCATGAGGTAGTTGCTTATCTGCTTGTTTACGTTACCACACTGCATTTCAGAAATCGTCTAACTTAATCAACTACCGGGTTAAACAGGTATCGATTCTCTGCAACGACTGTAGCCATACGAGCAATGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGTAGAAGGTCAATGAAAACTAAAGGTAAGCAAGAAAAATTGGGGCTCTCTTGATTTAACCATTAGGTTCTGTTCAAAGTTCGATTGTTCGAGTCATTTGATCCATTTAGAAGTTCGATTGTTTGAGTCATTTGATCCATTTAGGAAGGCAACCTGTTTTGTTCAGAGGCCGGCTATGATTGGTTTATACCCTTAATCAGACATGTGGAAAGTCTAATAGTGCAACATAAAGTTAAATAGTTGGATCTTCTATAGGAAGAGTCTCAGATTCTTTTCATTGTATCATTTAGTATTTATCTTGCAAAAAAATATATACATCATATTAGATACATTACGCAGATGTGAATTTATCATAAATCTCAACAAATCCTTGTTTGAATACCTGAATTTCAGTAAATTAAGAAATAGGTTGATGATAGTTCATTCGAGAAGATATAGTTAAGGGGATGGATGATAGAGTGCTAACTCTACTGATCGATTGGTTGCCGAAGAAACTCGTAGGATATTAAATCTTAGGTAAGTGTGGTCACCATTGGAACTCGTTCCCTCTAAACCTTTTATTATTTCCATTGCTCTTCTTGACCATTGGGCTGATCCTTGGTTGTGTAGTGCGATCAATATTAAACCCTACCAACCAATATATACACTACCAATAATTTGTTATGTATTTGTCCCTAGTATACGAGGGGTACAATGGATGTTCATTATCATTTTCGTTCGGTACTACAGAAAGGGAG

mRNA sequence

ATGGCAGCGATTAAAGCCCTACTTTCCATTGCTCTTATCGCTCTTCTCTTGTTCTTCGAATCCCTTCCAACTTCCGCCGCCGTTCACCGCGGGGCTTCCACTTCTCTTGCTGGAATACTTCGCCGGAACATCGTACAGACGACGGCGGATCATCATCAAGTTGCAGAGAAGAAGTTCAACGTTCATATCAAAAGGAGGATGCGGATGCGACCTGTGCTAATTGGCCGCCCTAAGACATCGAAGGCGAATCGGAGCCGAGAAGCAGAGAACGAACAGGGTTGGTCTCCAATGGAACAAGACGACTGCCCTGTGCCCCAATCTGCCGGGAGAGAAGATTTCGGGAAATTGCAGCACGGGTACCGATTTCTTTCCTTCATGAGGCGGCCTTGGCCGTCTTCTTCTTGTTCGTCCTCGTTTTCGTCATCAGTAACTTCTTTTCGATTTGAATTAGATGCGAGCATTATAGGAGACGATGCAGAATCCGAGCCCCGTGCTGCGACGGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGCTCGCTGAGCAATCCGAAGGACCGCCACGAACTTGTTCGACAGGACGTTAGACAAGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGCCGTCAGGGTTTGTCGCAACTGTAATGTTAATATGGGAGAATACTTTTGCGATATCTGCAAATTCTACGATGATGATACCAAAAAGAAGCAGTTTCATTGTGATGCTTGTGGTATCTGTAGAGTTGGTGGTCGTGAAAACTTCTTTCACTGTGAAAAGTGTGGATCGTGCTATTCCACCCCGCTGCGCGATAACCACATGTGTGTTGAGAACTCTATGAAAAGTTTTTGTCCAATCTGTTATGAGTTTCTTTTTGACTCAATTAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAGATGGAATCACAGAATCAATACCGTTGTCCTATCTGCTCCAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGATTGACTGCACACCGATGCCTGAGGAATACAGCCATGAGGTATCGATTCTCTGCAACGACTGTAGCCATACGAGCAATGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGTAGAAGGTCAATGAAAACTAAAGGTAAGCAAGAAAAATTGGGGCTCTCTTGATTTAACCATTAGGTTCTGTTCAAAGTTCGATTGTTCGAGTCATTTGATCCATTTAGAAGTTCGATTGTTTGAGTCATTTGATCCATTTAGGAAGGCAACCTGTTTTGTTCAGAGGCCGGCTATGATTGGTTTATACCCTTAATCAGACATGTGGAAAGTCTAATAGTGCAACATAAAGTTAAATAGTTGGATCTTCTATAGGAAGAGTCTCAGATTCTTTTCATTGTATCATTTAGTATTTATCTTGCAAAAAAATATATACATCATATTAGATACATTACGCAGATGTGAATTTATCATAAATCTCAACAAATCCTTGTTTGAATACCTGAATTTCAGTAAATTAAGAAATAGGTTGATGATAGTTCATTCGAGAAGATATAGTTAAGGGGATGGATGATAGAGTGCTCGTAGGATATTAAATCTTAGGTAAGTGTGGTCACCATTGGAACTCGTTCCCTCTAAACCTTTTATTATTTCCATTGCTCTTCTTGACCATTGGGCTGATCCTTGGTTGTGTAGTGCGATCAATATTAAACCCTACCAACCAATATATACACTACCAATAATTTGTTATGTATTTGTCCCTAGTATACGAGGGGTACAATGGATGTTCATTATCATTTTCGTTCGGTACTACAGAAAGGGAG

Coding sequence (CDS)

ATGGCAGCGATTAAAGCCCTACTTTCCATTGCTCTTATCGCTCTTCTCTTGTTCTTCGAATCCCTTCCAACTTCCGCCGCCGTTCACCGCGGGGCTTCCACTTCTCTTGCTGGAATACTTCGCCGGAACATCGTACAGACGACGGCGGATCATCATCAAGTTGCAGAGAAGAAGTTCAACGTTCATATCAAAAGGAGGATGCGGATGCGACCTGTGCTAATTGGCCGCCCTAAGACATCGAAGGCGAATCGGAGCCGAGAAGCAGAGAACGAACAGGGTTGGTCTCCAATGGAACAAGACGACTGCCCTGTGCCCCAATCTGCCGGGAGAGAAGATTTCGGGAAATTGCAGCACGGGTACCGATTTCTTTCCTTCATGAGGCGGCCTTGGCCGTCTTCTTCTTGTTCGTCCTCGTTTTCGTCATCAGTAACTTCTTTTCGATTTGAATTAGATGCGAGCATTATAGGAGACGATGCAGAATCCGAGCCCCGTGCTGCGACGGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGCTCGCTGAGCAATCCGAAGGACCGCCACGAACTTGTTCGACAGGACGTTAGACAAGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGCCGTCAGGGTTTGTCGCAACTGTAATGTTAATATGGGAGAATACTTTTGCGATATCTGCAAATTCTACGATGATGATACCAAAAAGAAGCAGTTTCATTGTGATGCTTGTGGTATCTGTAGAGTTGGTGGTCGTGAAAACTTCTTTCACTGTGAAAAGTGTGGATCGTGCTATTCCACCCCGCTGCGCGATAACCACATGTGTGTTGAGAACTCTATGAAAAGTTTTTGTCCAATCTGTTATGAGTTTCTTTTTGACTCAATTAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAGATGGAATCACAGAATCAATACCGTTGTCCTATCTGCTCCAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGATTGACTGCACACCGATGCCTGAGGAATACAGCCATGAGGTATCGATTCTCTGCAACGACTGTAGCCATACGAGCAATGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGTAGAAGGTCAATGA

Protein sequence

MAAIKALLSIALIALLLFFESLPTSAAVHRGASTSLAGILRRNIVQTTADHHQVAEKKFNVHIKRRMRMRPVLIGRPKTSKANRSREAENEQGWSPMEQDDCPVPQSAGREDFGKLQHGYRFLSFMRRPWPSSSCSSSFSSSVTSFRFELDASIIGDDAESEPRAATESLPVAIATTRPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRISTVEGQ

Homology

[Close]

BLAST of Carg22161 vs. NCBI nr
Match: KAG7025561.1 (E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 819.7 bits (2116), Expect = 1.1e-233
Identity = 398/398 (100.00%), Postives = 398/398 (100.00%), Query Frame = 0


Query: 1   MAAIKALLSIALIALLLFFESLPTSAAVHRGASTSLAGILRRNIVQTTADHHQVAEKKFN 60
           MAAIKALLSIALIALLLFFESLPTSAAVHRGASTSLAGILRRNIVQTTADHHQVAEKKFN
Sbjct: 1   MAAIKALLSIALIALLLFFESLPTSAAVHRGASTSLAGILRRNIVQTTADHHQVAEKKFN 60

Query: 61  VHIKRRMRMRPVLIGRPKTSKANRSREAENEQGWSPMEQDDCPVPQSAGREDFGKLQHGY 120
           VHIKRRMRMRPVLIGRPKTSKANRSREAENEQGWSPMEQDDCPVPQSAGREDFGKLQHGY
Sbjct: 61  VHIKRRMRMRPVLIGRPKTSKANRSREAENEQGWSPMEQDDCPVPQSAGREDFGKLQHGY 120

Query: 121 RFLSFMRRPWPSSSCSSSFSSSVTSFRFELDASIIGDDAESEPRAATESLPVAIATTRPC 180
           RFLSFMRRPWPSSSCSSSFSSSVTSFRFELDASIIGDDAESEPRAATESLPVAIATTRPC
Sbjct: 121 RFLSFMRRPWPSSSCSSSFSSSVTSFRFELDASIIGDDAESEPRAATESLPVAIATTRPC 180

Query: 181 SLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQ 240
           SLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQ
Sbjct: 181 SLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQ 240

Query: 241 FHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDT 300
           FHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDT
Sbjct: 241 FHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDT 300

Query: 301 TVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVS 360
           TVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVS
Sbjct: 301 TVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVS 360

Query: 361 ILCNDCSHTSNVRFHILGHKCSQCNSFNTRRISTVEGQ 399
           ILCNDCSHTSNVRFHILGHKCSQCNSFNTRRISTVEGQ
Sbjct: 361 ILCNDCSHTSNVRFHILGHKCSQCNSFNTRRISTVEGQ 398

[Close]

BLAST of Carg22161 vs. NCBI nr
Match: XP_023004622.1 (E3 ubiquitin-protein ligase MIEL1 [Cucurbita maxima] >XP_023513585.1 E3 ubiquitin-protein ligase MIEL1 [Cucurbita pepo subsp. pepo] >KAG6593162.1 E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 508.4 bits (1308), Expect = 5.4e-140
Identity = 248/305 (81.31%), Postives = 252/305 (82.62%), Query Frame = 0


Query: 97  MEQDDCPVPQSAGREDFGKLQHG---YRFLSFMRRPWPSSSCSSSFSSSVTSFRFELDAS 156
           MEQDDCPVPQSAGREDFGKLQHG   YR    +R P     C   F+             
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAP----CCDGIFTCR----------- 60

Query: 157 IIGDDAESEPRAATESLPVAIATTRPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV 216
                         E++          SLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV
Sbjct: 61  ----------HCHNEAM---------SSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV 120

Query: 217 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 276
           CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD
Sbjct: 121 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 180

Query: 277 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 336
           NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM
Sbjct: 181 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 240

Query: 337 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 396
           DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS
Sbjct: 241 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 271

Query: 397 TVEGQ 399
           TVEGQ
Sbjct: 301 TVEGQ 271

[Close]

BLAST of Carg22161 vs. NCBI nr
Match: XP_022959587.1 (E3 ubiquitin-protein ligase MIEL1 [Cucurbita moschata])

HSP 1 Score: 505.0 bits (1299), Expect = 6.0e-139
Identity = 246/305 (80.66%), Postives = 251/305 (82.30%), Query Frame = 0


Query: 97  MEQDDCPVPQSAGREDFGKLQHG---YRFLSFMRRPWPSSSCSSSFSSSVTSFRFELDAS 156
           MEQDDCPVPQSAGREDFGKLQHG   YR    +R P     C   F+             
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAP----CCDGIFTCR----------- 60

Query: 157 IIGDDAESEPRAATESLPVAIATTRPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV 216
                         E++          SLSNP DRHELVRQDVRQVVCLICNTEQEAVRV
Sbjct: 61  ----------HCHNEAM---------SSLSNPNDRHELVRQDVRQVVCLICNTEQEAVRV 120

Query: 217 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 276
           CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD
Sbjct: 121 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 180

Query: 277 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 336
           NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM
Sbjct: 181 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 240

Query: 337 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 396
           DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCS+CNSFNTRRIS
Sbjct: 241 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSRCNSFNTRRIS 271

Query: 397 TVEGQ 399
           TVEGQ
Sbjct: 301 TVEGQ 271

[Close]

BLAST of Carg22161 vs. NCBI nr
Match: XP_022953225.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata] >KAG6575860.1 E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 474.9 bits (1221), Expect = 6.6e-130
Identity = 232/301 (77.08%), Postives = 239/301 (79.40%), Query Frame = 0


Query: 97  MEQDDCPVPQSAGREDFGKLQHG---YRFLSFMRRPWPSSSCSSSFSSSVTSFRFELDAS 156
           MEQ+D PVPQSAGREDFGK QHG   YR    +R P     C   F+             
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAP----CCDRIFTCR----------- 60

Query: 157 IIGDDAESEPRAATESLPVAIATTRPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV 216
                         E++          SLSNPKDRHELVRQDVRQVVCLICNTEQE  + 
Sbjct: 61  ----------HCHNEAM---------TSLSNPKDRHELVRQDVRQVVCLICNTEQEVAKS 120

Query: 217 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 276
           C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD
Sbjct: 121 CCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 180

Query: 277 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 336
           NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM
Sbjct: 181 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 240

Query: 337 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 395
           DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTS VRFHILGHKCSQCNSFNTRRIS
Sbjct: 241 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSKVRFHILGHKCSQCNSFNTRRIS 267

[Close]

BLAST of Carg22161 vs. NCBI nr
Match: XP_022992470.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita maxima])

HSP 1 Score: 473.8 bits (1218), Expect = 1.5e-129
Identity = 231/301 (76.74%), Postives = 239/301 (79.40%), Query Frame = 0


Query: 97  MEQDDCPVPQSAGREDFGKLQHG---YRFLSFMRRPWPSSSCSSSFSSSVTSFRFELDAS 156
           MEQ+D PVPQSAGREDFGK QHG   YR    +R P     C   F+             
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAP----CCDRIFTCR----------- 60

Query: 157 IIGDDAESEPRAATESLPVAIATTRPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV 216
                         E++          SLSNPKDRH+LVRQDVRQVVCLICNTEQE  + 
Sbjct: 61  ----------HCHNEAM---------TSLSNPKDRHDLVRQDVRQVVCLICNTEQEVSKS 120

Query: 217 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 276
           C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD
Sbjct: 121 CSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 180

Query: 277 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 336
           NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM
Sbjct: 181 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 240

Query: 337 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 395
           DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTS VRFHILGHKCSQCNSFNTRRIS
Sbjct: 241 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSKVRFHILGHKCSQCNSFNTRRIS 267

[Close]

BLAST of Carg22161 vs. ExPASy Swiss-Prot
Match: Q8VZK0 (E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.2e-86
Identity = 138/214 (64.49%), Postives = 172/214 (80.37%), Query Frame = 0


Query: 181 SLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQ 240
           +L N  DRH+LVRQDV+QV+C +C+TEQ A +VC NC VNMGEYFC IC FYDDDT+K+Q
Sbjct: 48  TLRNIYDRHDLVRQDVKQVICSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQ 107

Query: 241 FHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDT 300
           FHCD CGICRVGGRENFFHC+KCGSCY+  LR+NH CVENSM+  CPICYE+LFDS+KDT
Sbjct: 108 FHCDDCGICRVGGRENFFHCKKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDT 167

Query: 301 TVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY-SHEV 360
            VM CGHT+H+EC+ EM  ++++ CPICS++V+DMS +W  L  EI+ T MP +Y   +V
Sbjct: 168 NVMKCGHTMHVECYNEMIKRDKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKV 227

Query: 361 SILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 394
            ILCNDC+ T+ V FHI+G KC  C S+NTR I+
Sbjct: 228 WILCNDCNDTTEVHFHIIGQKCGHCRSYNTRAIA 261

[Close]

BLAST of Carg22161 vs. ExPASy Swiss-Prot
Match: Q9FFB6 (E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=1 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.3e-72
Identity = 114/205 (55.61%), Postives = 148/205 (72.20%), Query Frame = 0


Query: 188 RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 247
           RHEL R +V +V+C +C TEQ+  + C NC V MG+YFC  CKF+DDD  KKQ+HCD CG
Sbjct: 65  RHELPRHEVSKVICSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECG 124

Query: 248 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 307
           ICR GG ENFFHC++C  CYS  + D H CVE +M   CP+C+E+LFDS +D TV+ CGH
Sbjct: 125 ICRTGGEENFFHCKRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGH 184

Query: 308 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDC 367
           T+HLEC ++M   N+Y CP+CSK++ DMS  W  L  E+   PMP+ Y ++ V ILCNDC
Sbjct: 185 TMHLECTKDMGLHNRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDC 244

Query: 368 SHTSNVRFHILGHKCSQCNSFNTRR 392
              +NVRFH++ HKCS C S+NTR+
Sbjct: 245 GSNTNVRFHLIAHKCSSCGSYNTRQ 269

[Close]

BLAST of Carg22161 vs. ExPASy Swiss-Prot
Match: Q10LI1 (E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SRFP1 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 6.5e-72
Identity = 116/216 (53.70%), Postives = 149/216 (68.98%), Query Frame = 0


Query: 178 RPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTK 237
           R C   + KD HEL R  V  V+CL+C+TEQ   +VC NC V MGEYFC  CKF+DDD  
Sbjct: 39  RHCHNESTKDGHELDRHAVESVICLVCDTEQPVAQVCYNCGVCMGEYFCSACKFFDDDVD 98

Query: 238 KKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSI 297
           ++ FHC  CGICRVGG++NFFHCEKCGSCYS  LRD H C+ENSMK+ CPICYE+LFDS+
Sbjct: 99  REHFHCQDCGICRVGGKDNFFHCEKCGSCYSVSLRDKHCCIENSMKNNCPICYEYLFDSL 158

Query: 298 KDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSH 357
           ++T+V+ CGHT+HL+CF EM   +++ CPICS  + DM      L  EI+   +  +Y  
Sbjct: 159 RETSVLRCGHTMHLQCFHEMLKHDKFSCPICSMPIFDMDKFLRALDAEIEANMLHIDYMG 218

Query: 358 EVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 394
           +  I+CNDC  T+ V   + GHKC  C S NT R++
Sbjct: 219 KGWIVCNDCRDTTQVYARVAGHKCCHCQSHNTCRVA 254

[Close]

BLAST of Carg22161 vs. ExPASy Swiss-Prot
Match: Q5JL96 (Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=39947 GN=RZFP34 PE=2 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.5e-71
Identity = 114/205 (55.61%), Postives = 152/205 (74.15%), Query Frame = 0


Query: 188 RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 247
           RHEL R +V+QV+C +C TEQE  +VC +C V MG+YFC++CK +DDD  K+Q+HC+ CG
Sbjct: 99  RHELPRHEVQQVICSLCGTEQEVRQVCISCGVCMGKYFCEVCKLFDDDVSKQQYHCNGCG 158

Query: 248 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 307
           ICR+GG+ENFFHC KCG CYS  L+++H CVE +M   CPIC+E+LF+S  D +V+PCGH
Sbjct: 159 ICRIGGKENFFHCSKCGCCYSIVLKNSHACVEGAMHHDCPICFEYLFESTNDVSVLPCGH 218

Query: 308 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 367
           TIH++C REME   Q+ CP+CSK+V DMS +W  L  E+  T      +  V ILCNDC 
Sbjct: 219 TIHVKCLREMEEHCQFACPLCSKSVCDMSKAWERLDEEL-ATISDTCDNKMVRILCNDCG 278

Query: 368 HTSNVRFHILGHKCSQCNSFNTRRI 393
            TS V+FH++ HKC +C S+NTR+I
Sbjct: 279 ATSEVQFHLIAHKCQKCKSYNTRQI 302

[Close]

BLAST of Carg22161 vs. ExPASy Swiss-Prot
Match: Q9CR50 (RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=10090 GN=Rchy1 PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.1e-54
Identity = 99/215 (46.05%), Postives = 135/215 (62.79%), Query Frame = 0


Query: 176 TTRPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDD 235
           T R C  +N  + H+L R  V++V C+ C   Q A + C +C+   GEY+C IC  +D D
Sbjct: 39  TCRLCHDTN--EDHQLDRFKVKEVQCINCEKLQHAQQTCEDCSTLFGEYYCSICHLFDKD 98

Query: 236 TKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFD 295
             K+Q+HC++CGICR+G +E+FFHC KC  C +T LR  H C+EN  +  CPIC E +  
Sbjct: 99  --KRQYHCESCGICRIGPKEDFFHCLKCNLCLTTNLRGKHKCIENVSRQNCPICLEDIHT 158

Query: 296 SIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY 355
           S     V+PCGH +H  C+ EM  +  YRCP+C  + +DM+  W  L  E+  TPMP EY
Sbjct: 159 SRVVAHVLPCGHLLHRTCYEEMLKEG-YRCPLCMHSALDMTRYWRQLDTEVAQTPMPSEY 218

Query: 356 SH-EVSILCNDCSHTSNVRFHILGHKCSQCNSFNT 390
            +  V ILCNDC+  S V+FHILG KC  C+S+NT
Sbjct: 219 QNVTVDILCNDCNGRSTVQFHILGMKCKLCDSYNT 248

[Close]

BLAST of Carg22161 vs. ExPASy TrEMBL
Match: A0A6J1KQY2 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.6e-140
Identity = 248/305 (81.31%), Postives = 252/305 (82.62%), Query Frame = 0


Query: 97  MEQDDCPVPQSAGREDFGKLQHG---YRFLSFMRRPWPSSSCSSSFSSSVTSFRFELDAS 156
           MEQDDCPVPQSAGREDFGKLQHG   YR    +R P     C   F+             
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAP----CCDGIFTCR----------- 60

Query: 157 IIGDDAESEPRAATESLPVAIATTRPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV 216
                         E++          SLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV
Sbjct: 61  ----------HCHNEAM---------SSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV 120

Query: 217 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 276
           CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD
Sbjct: 121 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 180

Query: 277 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 336
           NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM
Sbjct: 181 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 240

Query: 337 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 396
           DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS
Sbjct: 241 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 271

Query: 397 TVEGQ 399
           TVEGQ
Sbjct: 301 TVEGQ 271

[Close]

BLAST of Carg22161 vs. ExPASy TrEMBL
Match: A0A6J1H4Y6 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita moschata OX=3662 GN=LOC111460617 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.9e-139
Identity = 246/305 (80.66%), Postives = 251/305 (82.30%), Query Frame = 0


Query: 97  MEQDDCPVPQSAGREDFGKLQHG---YRFLSFMRRPWPSSSCSSSFSSSVTSFRFELDAS 156
           MEQDDCPVPQSAGREDFGKLQHG   YR    +R P     C   F+             
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAP----CCDGIFTCR----------- 60

Query: 157 IIGDDAESEPRAATESLPVAIATTRPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV 216
                         E++          SLSNP DRHELVRQDVRQVVCLICNTEQEAVRV
Sbjct: 61  ----------HCHNEAM---------SSLSNPNDRHELVRQDVRQVVCLICNTEQEAVRV 120

Query: 217 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 276
           CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD
Sbjct: 121 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 180

Query: 277 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 336
           NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM
Sbjct: 181 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 240

Query: 337 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 396
           DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCS+CNSFNTRRIS
Sbjct: 241 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSRCNSFNTRRIS 271

Query: 397 TVEGQ 399
           TVEGQ
Sbjct: 301 TVEGQ 271

[Close]

BLAST of Carg22161 vs. ExPASy TrEMBL
Match: A0A6J1GP21 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita moschata OX=3662 GN=LOC111455836 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 3.2e-130
Identity = 232/301 (77.08%), Postives = 239/301 (79.40%), Query Frame = 0


Query: 97  MEQDDCPVPQSAGREDFGKLQHG---YRFLSFMRRPWPSSSCSSSFSSSVTSFRFELDAS 156
           MEQ+D PVPQSAGREDFGK QHG   YR    +R P     C   F+             
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAP----CCDRIFTCR----------- 60

Query: 157 IIGDDAESEPRAATESLPVAIATTRPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV 216
                         E++          SLSNPKDRHELVRQDVRQVVCLICNTEQE  + 
Sbjct: 61  ----------HCHNEAM---------TSLSNPKDRHELVRQDVRQVVCLICNTEQEVAKS 120

Query: 217 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 276
           C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD
Sbjct: 121 CCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 180

Query: 277 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 336
           NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM
Sbjct: 181 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 240

Query: 337 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 395
           DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTS VRFHILGHKCSQCNSFNTRRIS
Sbjct: 241 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSKVRFHILGHKCSQCNSFNTRRIS 267

[Close]

BLAST of Carg22161 vs. ExPASy TrEMBL
Match: A0A6J1JVS4 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita maxima OX=3661 GN=LOC111488790 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 7.1e-130
Identity = 231/301 (76.74%), Postives = 239/301 (79.40%), Query Frame = 0


Query: 97  MEQDDCPVPQSAGREDFGKLQHG---YRFLSFMRRPWPSSSCSSSFSSSVTSFRFELDAS 156
           MEQ+D PVPQSAGREDFGK QHG   YR    +R P     C   F+             
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAP----CCDRIFTCR----------- 60

Query: 157 IIGDDAESEPRAATESLPVAIATTRPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV 216
                         E++          SLSNPKDRH+LVRQDVRQVVCLICNTEQE  + 
Sbjct: 61  ----------HCHNEAM---------TSLSNPKDRHDLVRQDVRQVVCLICNTEQEVSKS 120

Query: 217 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 276
           C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD
Sbjct: 121 CSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 180

Query: 277 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 336
           NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM
Sbjct: 181 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 240

Query: 337 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 395
           DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTS VRFHILGHKCSQCNSFNTRRIS
Sbjct: 241 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSKVRFHILGHKCSQCNSFNTRRIS 267

[Close]

BLAST of Carg22161 vs. ExPASy TrEMBL
Match: A0A6J1D947 (E3 ubiquitin-protein ligase MIEL1 OS=Momordica charantia OX=3673 GN=LOC111018400 PE=4 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.8e-128
Identity = 229/301 (76.08%), Postives = 239/301 (79.40%), Query Frame = 0


Query: 97  MEQDDCPVPQSAGREDFGKLQHG---YRFLSFMRRPWPSSSCSSSFSSSVTSFRFELDAS 156
           ME++D  VPQSA REDFGK QHG   YR    +R P     C   F+             
Sbjct: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAP----CCDRIFTCR----------- 60

Query: 157 IIGDDAESEPRAATESLPVAIATTRPCSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRV 216
                         E++          SLSNPKDRHELVRQDVRQVVCLICNTEQE  +V
Sbjct: 61  ----------HCHNEAM---------SSLSNPKDRHELVRQDVRQVVCLICNTEQEVAKV 120

Query: 217 CRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 276
           CR CNVNMGEYFCDICKF+DDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD
Sbjct: 121 CRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRD 180

Query: 277 NHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVM 336
           NH+CVENSMKSFCPICYEFLFDSIKDTTVM CGHTIHL+CFREMESQNQYRCPICSKTVM
Sbjct: 181 NHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGHTIHLDCFREMESQNQYRCPICSKTVM 240

Query: 337 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 395
           DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTS VRFHILGHKCSQCNSFNTRRIS
Sbjct: 241 DMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSKVRFHILGHKCSQCNSFNTRRIS 267

[Close]

BLAST of Carg22161 vs. TAIR 10
Match: AT3G62970.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 339.0 bits (868), Expect = 5.3e-93
Identity = 144/214 (67.29%), Postives = 172/214 (80.37%), Query Frame = 0


Query: 181 SLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQ 240
           SL +PK+RH+LVRQ+V+QVVC IC TEQE  +VC NC VNMGEYFCDICKF+DDD  K+Q
Sbjct: 65  SLPDPKERHDLVRQNVKQVVCSICQTEQEVAKVCSNCGVNMGEYFCDICKFFDDDISKEQ 124

Query: 241 FHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDT 300
           FHCD CGICRVGGR+ FFHC+ CG+CY   LRD H C+ENS K+ CP+CYE+LFDS+K  
Sbjct: 125 FHCDDCGICRVGGRDKFFHCQNCGACYGMGLRDKHSCIENSTKNSCPVCYEYLFDSVKAA 184

Query: 301 TVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVS 360
            VM CGHT+H++CF +M ++NQYRCPIC+K+++DMS SW LL  EI  T MP EY  EVS
Sbjct: 185 HVMKCGHTMHMDCFEQMINENQYRCPICAKSMVDMSPSWHLLDFEISATEMPVEYKFEVS 244

Query: 361 ILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIST 395
           ILCNDC+  S   FHILGHKCS C S+NTRRIST
Sbjct: 245 ILCNDCNKGSKAMFHILGHKCSDCGSYNTRRIST 278

[Close]

BLAST of Carg22161 vs. TAIR 10
Match: AT5G18650.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 321.6 bits (823), Expect = 8.7e-88
Identity = 138/214 (64.49%), Postives = 172/214 (80.37%), Query Frame = 0


Query: 181 SLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQ 240
           +L N  DRH+LVRQDV+QV+C +C+TEQ A +VC NC VNMGEYFC IC FYDDDT+K+Q
Sbjct: 48  TLRNIYDRHDLVRQDVKQVICSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQ 107

Query: 241 FHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDT 300
           FHCD CGICRVGGRENFFHC+KCGSCY+  LR+NH CVENSM+  CPICYE+LFDS+KDT
Sbjct: 108 FHCDDCGICRVGGRENFFHCKKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDT 167

Query: 301 TVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY-SHEV 360
            VM CGHT+H+EC+ EM  ++++ CPICS++V+DMS +W  L  EI+ T MP +Y   +V
Sbjct: 168 NVMKCGHTMHVECYNEMIKRDKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKV 227

Query: 361 SILCNDCSHTSNVRFHILGHKCSQCNSFNTRRIS 394
            ILCNDC+ T+ V FHI+G KC  C S+NTR I+
Sbjct: 228 WILCNDCNDTTEVHFHIIGQKCGHCRSYNTRAIA 261

[Close]

BLAST of Carg22161 vs. TAIR 10
Match: AT5G25560.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 284.6 bits (727), Expect = 1.2e-76
Identity = 120/207 (57.97%), Postives = 148/207 (71.50%), Query Frame = 0


Query: 186 KDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDA 245
           K RH++ R  V QV+CL+C TEQE  ++C +C V MG+YFC +CK YDDDT KKQ+HCD 
Sbjct: 99  KQRHDIPRHQVEQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDG 158

Query: 246 CGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPC 305
           CGICR+GGRENFFHC KCG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+PC
Sbjct: 159 CGICRIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPC 218

Query: 306 GHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCN 365
           GHTIH +C  EM    QY CP+CSK+V DMS  W    MEI  TPMPE Y +  V ILCN
Sbjct: 219 GHTIHQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCN 278

Query: 366 DCSHTSNVRFHILGHKCSQCNSFNTRR 392
           DC   + V++H++  KC  C S+NTR+
Sbjct: 279 DCGKKAEVQYHVVAQKCPNCKSYNTRQ 305

[Close]

BLAST of Carg22161 vs. TAIR 10
Match: AT5G25560.4 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 284.6 bits (727), Expect = 1.2e-76
Identity = 120/207 (57.97%), Postives = 148/207 (71.50%), Query Frame = 0


Query: 186 KDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDA 245
           K RH++ R  V QV+CL+C TEQE  ++C +C V MG+YFC +CK YDDDT KKQ+HCD 
Sbjct: 94  KQRHDIPRHQVEQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDG 153

Query: 246 CGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPC 305
           CGICR+GGRENFFHC KCG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+PC
Sbjct: 154 CGICRIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPC 213

Query: 306 GHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCN 365
           GHTIH +C  EM    QY CP+CSK+V DMS  W    MEI  TPMPE Y +  V ILCN
Sbjct: 214 GHTIHQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCN 273

Query: 366 DCSHTSNVRFHILGHKCSQCNSFNTRR 392
           DC   + V++H++  KC  C S+NTR+
Sbjct: 274 DCGKKAEVQYHVVAQKCPNCKSYNTRQ 300

[Close]

BLAST of Carg22161 vs. TAIR 10
Match: AT5G25560.3 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 276.6 bits (706), Expect = 3.2e-74
Identity = 120/217 (55.30%), Postives = 148/217 (68.20%), Query Frame = 0


Query: 186 KDRHELVRQDVR----------QVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDD 245
           K RH++ R  V           QV+CL+C TEQE  ++C +C V MG+YFC +CK YDDD
Sbjct: 99  KQRHDIPRHQVEQLTRPLSSSSQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDD 158

Query: 246 TKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFD 305
           T KKQ+HCD CGICR+GGRENFFHC KCG CYS  L++ H CVE +M   CPIC+EFLF+
Sbjct: 159 TSKKQYHCDGCGICRIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFE 218

Query: 306 SIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY 365
           S  D TV+PCGHTIH +C  EM    QY CP+CSK+V DMS  W    MEI  TPMPE Y
Sbjct: 219 SRNDVTVLPCGHTIHQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPY 278

Query: 366 SHE-VSILCNDCSHTSNVRFHILGHKCSQCNSFNTRR 392
            +  V ILCNDC   + V++H++  KC  C S+NTR+
Sbjct: 279 QNRMVQILCNDCGKKAEVQYHVVAQKCPNCKSYNTRQ 315

The following BLAST results are available for this feature:

BLAST of Carg22161 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025561.1	1.1e-233	100.00	E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023004622.1	5.4e-140	81.31	E3 ubiquitin-protein ligase MIEL1 [Cucurbita maxima] >XP_023513585.1 E3 ubiquiti...	[more]
XP_022959587.1	6.0e-139	80.66	E3 ubiquitin-protein ligase MIEL1 [Cucurbita moschata]	[more]
XP_022953225.1	6.6e-130	77.08	E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata] >KAG6575860.1 E3 ubi...	[more]
XP_022992470.1	1.5e-129	76.74	E3 ubiquitin-protein ligase MIEL1-like [Cucurbita maxima]	[more]

BLAST of Carg22161 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZK0	1.2e-86	64.49	E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 ...	[more]
Q9FFB6	2.3e-72	55.61	E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=...	[more]
Q10LI1	6.5e-72	53.70	E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SR...	[more]
Q5JL96	1.5e-71	55.61	Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q9CR50	2.1e-54	46.05	RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=1...	[more]

BLAST of Carg22161 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KQY2	2.6e-140	81.31	E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE...	[more]
A0A6J1H4Y6	2.9e-139	80.66	E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita moschata OX=3662 GN=LOC111460617 ...	[more]
A0A6J1GP21	3.2e-130	77.08	E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1JVS4	7.1e-130	76.74	E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita maxima OX=3661 GN=LOC1114887...	[more]
A0A6J1D947	1.8e-128	76.08	E3 ubiquitin-protein ligase MIEL1 OS=Momordica charantia OX=3673 GN=LOC111018400...	[more]

BLAST of Carg22161 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62970.1	5.3e-93	67.29	zinc finger (C3HC4-type RING finger) family protein	[more]
AT5G18650.1	8.7e-88	64.49	CHY-type/CTCHY-type/RING-type Zinc finger protein	[more]
AT5G25560.1	1.2e-76	57.97	CHY-type/CTCHY-type/RING-type Zinc finger protein	[more]
AT5G25560.4	1.2e-76	57.97	CHY-type/CTCHY-type/RING-type Zinc finger protein	[more]
AT5G25560.3	3.2e-74	55.30	CHY-type/CTCHY-type/RING-type Zinc finger protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 286..328 e-value: 7.1E-8 score: 42.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 286..328 e-value: 7.1E-8 score: 32.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 286..329 score: 12.282736
None	No IPR available	GENE3D	2.20.28.10		coord: 356..398 e-value: 1.4E-17 score: 64.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 75..105
None	No IPR available	PANTHER	PTHR21319	RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1	coord: 183..392
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 285..336
IPR008913	Zinc finger, CHY-type	PFAM	PF05495	zf-CHY	coord: 185..231 e-value: 1.0E-9 score: 38.7
IPR008913	Zinc finger, CHY-type	PROSITE	PS51266	ZF_CHY	coord: 176..219 score: 9.230992
IPR039512	RCHY1, zinc-ribbon	PFAM	PF14599	zinc_ribbon_6	coord: 333..390 e-value: 3.7E-23 score: 81.3
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 267..338 e-value: 1.2E-10 score: 42.5
IPR031109	Ring finger and CHY zinc finger domain-containing protein, plant	PANTHER	PTHR21319:SF12	ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN	coord: 183..392
IPR017921	Zinc finger, CTCHY-type	PROSITE	PS51270	ZF_CTCHY	coord: 221..285 score: 33.139317
IPR037275	Zinc finger, CTCHY-type superfamily	SUPERFAMILY	161245	Zinc hairpin stack	coord: 222..279

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg22161-RA	Carg22161-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005634	nucleus
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding