Carg22156 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22156
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Peptide chain release factor 1
Location	Carg_Chr08: 2275741 .. 2283172 (+)
RNA-Seq Expression	Carg22156
Synteny	Carg22156

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGTACCAAACAGTGCTTCTAGTTCACCCTGCCAAACACCACACAACCACTTCACCACTCTATCTTTATCTTCTCGCTTCTGTTTCTGTTTGGAATCTCGGTAGCAGCGATGAATTTTTTCTCTCCGACCACCGCAAGTCGGACAATTCCGGGATGTTTTCTTTTTCCGCCACGATACGGTGACTTCTCGAGAAGATGTTCTCTGGAGCTTAAACGTTCTGCACCTCCGTTTAGAGCTTCAACTCTCGTTTGCATGGCGGTTCGTACATTTCCGACCGATAAATGTAGATAGATTTCCGGACGTTTTGTCCTCGAGAGGGAACATGTCATCTTATTGTTTGGATTGGTTTTAATTGAATTTCAGTTTCTTTATGTTTCGAGTTCACTATGTTTTCTTTCGCTGAAGTTATTGGAGATGAATTCGTGGCACAACTGGTCTGGCTGAGGTTTATGGCAAGTGATGTTTGTATTTTATTATTTCAGGAACCTTATTTGATCACGAAGCTGGAATCAGCCGAGAATACGTGGAAAGAGTTATCGGTACTCACTGATTTATGGTTCAATAGCATTTTAATTTGTTTGCTGAAGGTGCTTGTATCCATCAATTTTTTTAGCGTGGTTATAATTGGAACAAAATTTTGGCATGATTTTTAGCTTAGAAATTTATAGTTTAAAATGCCAATAAGTCTTAACTTCTGTCTCAATGTTGTTATAGTTTGACTACAACGATAATTGAACTTAGTTTTCTTCCGAGTGAAAATCCAGGGAAGGGGAAATTAGCTAAGATATGAAAGTTAAGAGCTCTTAGAATTATCATGCTCCATTATATTATGAATTTTCCTTTCATGAAGATATCAGGAAACGTAATTTGCAGTTAAAATGAAACCGTGGCTACAGTGAACTCTGTATATTGTTTCTGCATGACTGTAATTCTGCTGCGAACTTTTAAATTTTAAAATAAGTAAATTAAGTACCCAACGATTCCACTTAGTTACTTACTTTTAGAGTACTAACAACAAAAGAAGAGGTAGAAGAACGTCCTCACTAGAGACCTTGTCTATGTGCCTGGTATTATGATACTCTTTAGGCAAAAAATGAAGAAATGTATTTTGGATTTGACTATAGCCTATAATGATAAACTAATAATTCCTTGCTTTCCCACTACACTTGGGATTTGAATTGAATTTTTTTAGCTATCACGAGTATTTTTTTAACCACCGCTTTTCTCCTGAAAATTTTGTAAAACTAATGAAATGTTTATGAATAAAATCTAGGTGTATTAACTGTAATCAAGTTTCTCCCCTCATTCTGTAGGCCACATTTGGAACTTGCATTTACATTCTCAGTGCTCCAATTATGCTTTTCATTTGGTTATTGTTTTATTATTTTTCCCCACCAGAATCATAGTTGGTATATTTATTTAGGTCAAGCTGGCTGATCCAGATATTGTCTCGAACCCCACAGAGTATCAAAAACTTGCCCAATCAATGTCAGAGCTTGACGAGGTATACCACAACTATTTGCCTCTGATATTGTGATTTATAGGAACAGTTAGAAGAACCTTCCCCTTTTTTTCCCTTCAGATTGTTTCTTTTCAAAACTGACGTTACACTATTCCTATTGGTTGGTTGGTGTGGGATGCCGGGACAGGTGGTATCAACCTTCAGAAGATTCAAGGAATGTGAGAAGCAGTTGCAGGAGGCAAAAGGTTGACTGATAAGTACTTGAAAAAAATTTATGACAATTTTTTTTTTTGAATTTGTCTTATTTTCTTCTTCTTTGTCATGATTATCGTGTCAGCTTTAGCAAAAGAAAGTGGGAATGACGAGGATATGGCAGAGATGATTGCTTTAGAAATCAGTTCTTTATCCAGTCAACTCAAAGACATTGAGGAGAAGCTTAAGGTGCTTTTGATTCATGAACTTGATCATTACATGTGTGTATATTTTGGATAGGAGTCAAACTTTTATTAATGTTAATGTGCGTATAATTCTCTTTATCGATGTCGTTTTGTTTCCCTGTTTGGAGTGGGGTGGAGGTCCTTGATTTTCAATCCTTCACAGTTTGTGGTTCAGAGTTTTCATATTCTGCTTTTTAACGGGCATCCGTTCAGACAGATGTCTAAACGCACTCTATTTTGTTTCTTTAGCAATTTTCATCAGTTTTTGTTATTTTCTCTTCTTTTGTAAGTTGGTAGAATTATTTAATTTCTGAAACAAATAGGTAGTGGCATTGTAGCTTGGTTCTTTACTAATTGTCAGATTTTTACAAGCAAATACTGTGCTTTCATGTCATTTGTGTGGTTTGATTGTTTTCTTTTTTTGGGGACTTTAGTTATTTCCTGGTATTCTACAAGTTGCATCTTATGTTTCTCTGAATCTGAAGACATTATGTCACAATTTTCTTTATCAGGTGATGTTGCTTCCATCTGATCCTCTTGATGCAAGAAATATTATGCTTGAAGGTCAGCTTCCTCAGTTTCCAATTTTATGCCATCTGAATATGAAAAATTTTAAAGTCCATTTTCCGGAGGTTGCATAGATTCATTGTGCAAAAATGATATACTTATTGGACAATTAGAATTCTGTAAAAGTAAACTGCTTACACTTGTGTGTTTTGTTTATGCTGATTAAAAATTGTAATATTCTTCTTGCCATGCAGTCCGAGCAGGTACGGGTGGTGATGAAGCTGGAATCTGGGCTGGTGATCTTGTAGGTACATGCTTCTTTTATCCTTCAAGTACTACGGAAAATTGAGCACATATTCTTTTTTTGGTTGCCCTTACAATAGCCGAACATGAGAAATATTGGTTATAATAGAAAATCGCTTGATATCTTTGTAAGAAATGCTATGTCAAATAAACATATTATTTGAAGTGATCTATGATTTATATTCCCCTTTCTAACCTTTTAGGTCTTTTAAGTGTATTGAAAAAGTATTGATAGGCTTAGTGAACTTCCATGTTCTGAAGTTTCTTCCATATACATTCTTGCATTGCTTTGATTTTAGACCTCCGTTATCCTATGTATTGTTCTGTGTTACCATTTCTTCTCCTCTCATCTTTTCCATTTATGTAACAGGTCCGTATGTATCAGAAATACAGTGAGCGGAATTCTTGGAAATTTTCCCCTATTTCAAGTTCTGAGGTATGTGTTTCTAAACTTCCCAGAAGAAGTAAACATTCTAGTGCTTTCTGTATGTCTAAAAGAACTTATACTGTTCACCTTTCAGGCTGAAAAAGGTGGATTCAAAACTTATGTGTTGGAAATCAAAGGAAATCGTGTCTACAGTAAATTAAAATATGAGTCTGGTGTTCACCGGGTTCAACGTGTTCCTCAAACAGAAACACAGGGCCGTGTGCATACTTCCACTGCTACAGTAGCCATCATGCCAGAGGTAAACAATTTCCTGAATTTTCTGTTGAATGTTGATTAATTTTGTCTGATTCTGGTAAAAAAAGTATTGCATGCCCCAATCAGACTAATGTCTAGAATAACATCACGTGAGTTTGTTAATTTCCTCCTTGACAGTTGCTGATGTTAATTCGTGTAGTTTCCTTCTTGTTCCCCTTCCCTTAATAGGTTCCTGATCTATTTCTTGTACTCCGTTATTATCTGGATAGTCTTCAATACTTTTAGCACTTGACAATTATAACGTGGAAAAGTTGTACCGCAGCTTTAAAATCCCTAGACGGTGAATTAATGTGGGATTACTAATCACGGTTTTCTGACATATCTCACCAGTGCCTTAACATGAAGACCTGAGCAGATAACTGTAGACTTATCCTTCATATTTATTTAGTTGATGTTGATAGTGTCCATACTGTTTCTTTGTAGTAGTTATGATTTAGATATGATAACTTCTCTTGACTTGACTATGCATTCACATCTTGATCAGCTATATACTTTCTCTTGATTGGTTGAGCTTGTTTCATTCTTGCATCTAGGCAGATGAAGTTGAGGTTGTGATTGATCCAAAAGACATTGAACTAACAACTACAAGATCTGGGGGTGCAGGAGGTATAAATTTACTTAATTGATTCAAATATTACTTTGGCTAGCTCTCTGTATCTGTTCTCATAAACCAAGAAATGGTAGAATAACCTTTTTTTTTTCCAATGGTTATGCAGGGCAGAATGTGAACAAGGTGGAGACAGCAATTGATCTTTTCCACAAGCCAACAGGAATTCGCATTTTTTGCACTGAAGAAAGGACTCAACTTCAGAACAAGCATCGAGCTTTTCAATTGCTGCGAGCCAAACTGTGAGCATCCATTACACCATAAACATTTGCCATTTTATCATATAAATTATATGCACTTTATTCATATAATTCCTATATTTTTTGCCGAAACAAATAATTCAATGAACTTTCATGACCATTTCTAGGGTTTTCTCAAATAATTGAAGAAACTTTATTGTCATTGCAATATTCCGAAGGAATTTGGCTGGTCATTGGTATGGGGCAATAAAAGTAAAATAAAATAACCGAGATTGAACAGTGGTTCGTTCAGTTCTGCACAACCCTTGTGATCAACTGAGGCATCAAAGACATCTTAATTTTCTTTTTCTTTCTCTCACGCACAGCCTCCACCACCCATTGTTCACACCCAACCCGGGCCAGCCCCTTCCCCAACCTTTGTCCATGCTTGCCCTTGTATGTTCTGGCTTAGCCTCTCGCTTCCTATTTGCCATCCCCCTCCAATCCAACCTGGTCGGTTGGTCAGTTCTTTCGTCCAAAGAAAGCAGAAAAGTTAGTTTCTTATGCCCTGCCCCAACTGACAATCAGCTCAAGGGTACCTCTACCCTGATCTCCACTGACTTTCGCCCCTAGTCATTGGTTTCTTAACCCTCCTATTCAATCTCTTGGCATTAATTTTTCAAAAAGAAAATGATGTTTTTCTAATATATATTGACTTGCCTTTCATAGTGGACAGGGTGATAATTTTGCTGGTTTCAACATCGTGTAAATTCATGAATTTAATCCCCCTTCCCCTTCCCCCTTCTTTTCCTTTTCAGTTATGAGATCAAAGTGAGGGAACAGCAGGAGAGGATCAGGAATCAACGAAAATTACAGGTAAATATTAGTTTTCTCTTTCTTTCTTTTCTCGTTATCATTTATCCATGTTTAGCAAAGAACACCTTTTTGTGCATTTGGTTTTTTGAGTGTCCATGTACTTTTTCATTTTCTCTTAATGAAGGTTTGGTTCTTTATTAGAAAAAATCAGACAATAGTTTCTGAAGTTATTACAGTCAGTTAGTCGCTATATTTGATAAACTTGCCTAGGAAACGAAGAGCTGTTACCCTCCATTTTTTTCAAGCACTTCCTAGTCTAGAGTAATTTCTAAGTAAGCACACAATTTCATTGGCAACATTATTGGAAACGGCAATGTCCATTATGCAATACTATTTGGATGGAATATCAAAAACTAAAAATCTCTTGGACTCCACAAACCCATACCCAGAGGTCTTAGGTGCACAAATACGACTGTTTTAGAGCACGATAATTATATTTTAGGAATCTTTTATCAATTTTTTTCTTCTTTCTGCTGTGATAATAATCTACTTCATAATGTTGCCAAATCATTAGTTTTTGCTCAATTTTACAATATTCTACGTGTGTAACTCTAAATCTGAAAGCAAGTAGTTGTCACATTAATGCAAGGCAATTGTACAAGCAGACGCCAGCCGAATATTCTCATAAAAGCAATCGAGTATAAGCTGTTCTTCAATTTCAGTGTTTTTATTTTCTTGTTGTCCTCTTTTCTGTCTCCTCACAGACATTTTCAAAATTGGTTGTCAATTTTTTAATGATGAAACTTCATGCAAATCACTGCTTGGGGTTGTTGCAACTTGAATTTGTTAAACGAAATTGAATACCTGATTATAACTTCTCAATGTAATTTTATTAGGCAATCAAATTGTTTTCATTGGTGTATTCCCTCGTTCTAAAAGGTTCTGTTGCAGGTTGGTACAGGTGCCCGTGCTGAGAAAATACGGACTTACAATTACAAGGTATGATATTGGTTATATTTCTGGCTTGAAACTGATGAAAAAAGAAATACAGCTACTGTCATAAAGCAAGCTATCCATATGGTACCACAAAGATGTTGAAGACCAAGAAATAGTTCTAAAAGATAAATTAGTGCATGAAGTATTAACTTGTTATGAACGTTATATAAAAGCAACTATTATTGGTTGGCCTAGTGGACAAGAGAGTAATGGACCTATAGGGAACAAGTTCAGTCACGATCACCTAGACTTAATATTCGATAAGTATCTTGACAGCCAAATGTAGTAGGGTTAGACGGTTGTTCCATGAAATTAGTTGAGGTGCTAGCAAGCGTGTTTGGATCCTCATTGTTATATAAAAGAATAAAAAAAAAATTAGAAAAGGACGGAAAAGAATAAAAAAATTAAAAAAAGACGAAGGAAGGACCAAACCAAAAGGGTTTGAAAAACCGATCATGTCACAAACAAACTCACAAATTGTCATGACAACCATGTATACTCGTCTCACTAATGAAACGATCGAACTTGTGAAACTATCATTTCCAGTGTGTAGTTCAAGGTGGAAATGAAACATCTTAGCATAATTCATAGAAATTGATCCTAACAGACTGATAATTTCCATAGAATTCACTAACTTTTGGATTTTCCTCATATTAGGATAACAGGGTGACTGACCACAGGTTGAAAATGAACTTCGAGCTCACATCCTTTATTGATGGTGATATAGAGAACGCAGTTCAGGTAATATTCTCTCTCATCTTTTTACCTTTTGAACTCTCATTGAAAGCAAAAATTCACATCCTCCATTTTCTTTTTCAATCAAGGCATGTGTTGCCATGGAACAAAAAGAACTCATGGAAGAATTGGTTCAATCTACCGGGGCTCCAACCACTTGAATTTCCATCATTTCCTGCCAATAGTTTGGGCCCTCATAACACGTATTTCTTTGAGAACCGTATGAACTGCTATGAGGTTAGGTAAAAATCATTCACCATTATTCATAATTTTTCTGCACATGGGCCTGCCTGCTATATGTATGATTGATATACACATACTGAGATTATACCATTTAGAGTTGGGTTTTTGGCGTCAGTAGTCTGAAAATAGAGGGAGAAAAAGAAGAAATTAACCCATCCCATCCCCGCTAGAACTAGATTGTGATGTTGATGCGACTTTACGAAATTAGTTACATATTTTTCGTTTATTGTATGGTTTACGAAGTATTATTTCGATGAATTTTTCATTGTCTTGGTTGGTTTGGGGATTCTTGCAGTCTCACATGCAGTTTTGTTGATCTTTGACTGACTTGGTCCAGTTTGGTTGATCATGTTATGAAAAGGGTGATAGATACTAAAGATTCGAGCCATAGAATTTCTGAATTCTATCGACTCTCCACGATGGTATGATATTGTCCATTTTG

mRNA sequence

AATGTACCAAACAGTGCTTCTAGTTCACCCTGCCAAACACCACACAACCACTTCACCACTCTATCTTTATCTTCTCGCTTCTGTTTCTGTTTGGAATCTCGGTAGCAGCGATGAATTTTTTCTCTCCGACCACCGCAAGTCGGACAATTCCGGGATGTTTTCTTTTTCCGCCACGATACGGTGACTTCTCGAGAAGATGTTCTCTGGAGCTTAAACGTTCTGCACCTCCGTTTAGAGCTTCAACTCTCGTTTGCATGGCGGAACCTTATTTGATCACGAAGCTGGAATCAGCCGAGAATACGTGGAAAGAGTTATCGGTCAAGCTGGCTGATCCAGATATTGTCTCGAACCCCACAGAGTATCAAAAACTTGCCCAATCAATGTCAGAGCTTGACGAGGTGGTATCAACCTTCAGAAGATTCAAGGAATGTGAGAAGCAGTTGCAGGAGGCAAAAGCTTTAGCAAAAGAAAGTGGGAATGACGAGGATATGGCAGAGATGATTGCTTTAGAAATCAGTTCTTTATCCAGTCAACTCAAAGACATTGAGGAGAAGCTTAAGGTGATGTTGCTTCCATCTGATCCTCTTGATGCAAGAAATATTATGCTTGAAGTCCGAGCAGGTACGGGTGGTGATGAAGCTGGAATCTGGGCTGGTGATCTTGTCCGTATGTATCAGAAATACAGTGAGCGGAATTCTTGGAAATTTTCCCCTATTTCAAGTTCTGAGGCTGAAAAAGGTGGATTCAAAACTTATGTGTTGGAAATCAAAGGAAATCGTGTCTACAGTAAATTAAAATATGAGTCTGGTGTTCACCGGGTTCAACGTGTTCCTCAAACAGAAACACAGGGCCGTGTGCATACTTCCACTGCTACAGTAGCCATCATGCCAGAGGTAAACAATTTCCTGAATTTTCTGTTGAATGCAGATGAAGTTGAGGTTGTGATTGATCCAAAAGACATTGAACTAACAACTACAAGATCTGGGGGTGCAGGAGGGCAGAATGTGAACAAGGTGGAGACAGCAATTGATCTTTTCCACAAGCCAACAGGAATTCGCATTTTTTGCACTGAAGAAAGGACTCAACTTCAGAACAAGCATCGAGCTTTTCAATTGCTGCGAGCCAAACTTTATGAGATCAAAGTGAGGGAACAGCAGGAGAGGATCAGGAATCAACGAAAATTACAGGTTCTGTTGCAGGTTGGTACAGGTGCCCGTGCTGAGAAAATACGGACTTACAATTACAAGGATAACAGGGTGACTGACCACAGGTTGAAAATGAACTTCGAGCTCACATCCTTTATTGATGGTGATATAGAGAACGCAGTTCAGGCATGTGTTGCCATGGAACAAAAAGAACTCATGGAAGAATTGGTTCAATCTACCGGGGCTCCAACCACTTGAATTTCCATCATTTCCTGCCAATAGTTTGGGCCCTCATAACACGTATTTCTTTGAGAACCGTATGAACTGCTATGAGGTTAGGTAAAAATCATTCACCATTATTCATAATTTTTCTGCACATGGGCCTGCCTGCTATATGTATGATTGATATACACATACTGAGATTATACCATTTAGAGTTGGGTTTTTGGCGTCAGTAGTCTGAAAATAGAGGGAGAAAAAGAAGAAATTAACCCATCCCATCCCCGCTAGAACTAGATTGTGATGTTGATGCGACTTTACGAAATTAGTTACATATTTTTCGTTTATTGTATGGTTTACGAAGTATTATTTCGATGAATTTTTCATTGTCTTGGTTGGTTTGGGGATTCTTGCAGTCTCACATGCAGTTTTGTTGATCTTTGACTGACTTGGTCCAGTTTGGTTGATCATGTTATGAAAAGGGTGATAGATACTAAAGATTCGAGCCATAGAATTTCTGAATTCTATCGACTCTCCACGATGGTATGATATTGTCCATTTTG

Coding sequence (CDS)

ATGAATTTTTTCTCTCCGACCACCGCAAGTCGGACAATTCCGGGATGTTTTCTTTTTCCGCCACGATACGGTGACTTCTCGAGAAGATGTTCTCTGGAGCTTAAACGTTCTGCACCTCCGTTTAGAGCTTCAACTCTCGTTTGCATGGCGGAACCTTATTTGATCACGAAGCTGGAATCAGCCGAGAATACGTGGAAAGAGTTATCGGTCAAGCTGGCTGATCCAGATATTGTCTCGAACCCCACAGAGTATCAAAAACTTGCCCAATCAATGTCAGAGCTTGACGAGGTGGTATCAACCTTCAGAAGATTCAAGGAATGTGAGAAGCAGTTGCAGGAGGCAAAAGCTTTAGCAAAAGAAAGTGGGAATGACGAGGATATGGCAGAGATGATTGCTTTAGAAATCAGTTCTTTATCCAGTCAACTCAAAGACATTGAGGAGAAGCTTAAGGTGATGTTGCTTCCATCTGATCCTCTTGATGCAAGAAATATTATGCTTGAAGTCCGAGCAGGTACGGGTGGTGATGAAGCTGGAATCTGGGCTGGTGATCTTGTCCGTATGTATCAGAAATACAGTGAGCGGAATTCTTGGAAATTTTCCCCTATTTCAAGTTCTGAGGCTGAAAAAGGTGGATTCAAAACTTATGTGTTGGAAATCAAAGGAAATCGTGTCTACAGTAAATTAAAATATGAGTCTGGTGTTCACCGGGTTCAACGTGTTCCTCAAACAGAAACACAGGGCCGTGTGCATACTTCCACTGCTACAGTAGCCATCATGCCAGAGGTAAACAATTTCCTGAATTTTCTGTTGAATGCAGATGAAGTTGAGGTTGTGATTGATCCAAAAGACATTGAACTAACAACTACAAGATCTGGGGGTGCAGGAGGGCAGAATGTGAACAAGGTGGAGACAGCAATTGATCTTTTCCACAAGCCAACAGGAATTCGCATTTTTTGCACTGAAGAAAGGACTCAACTTCAGAACAAGCATCGAGCTTTTCAATTGCTGCGAGCCAAACTTTATGAGATCAAAGTGAGGGAACAGCAGGAGAGGATCAGGAATCAACGAAAATTACAGGTTCTGTTGCAGGTTGGTACAGGTGCCCGTGCTGAGAAAATACGGACTTACAATTACAAGGATAACAGGGTGACTGACCACAGGTTGAAAATGAACTTCGAGCTCACATCCTTTATTGATGGTGATATAGAGAACGCAGTTCAGGCATGTGTTGCCATGGAACAAAAAGAACTCATGGAAGAATTGGTTCAATCTACCGGGGCTCCAACCACTTGA

Protein sequence

MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLESAENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKESGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQVLLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEELVQSTGAPTT

Homology

[Close]

BLAST of Carg22156 vs. NCBI nr
Match: KAG7025567.1 (Peptide chain release factor APG3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 836.6 bits (2160), Expect = 9.4e-239
Identity = 430/430 (100.00%), Postives = 430/430 (100.00%), Query Frame = 0


Query: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60
           MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES
Sbjct: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60

Query: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120
           AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE
Sbjct: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120

Query: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN 300
           PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN
Sbjct: 241 PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN 300

Query: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV 360
           KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV
Sbjct: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV 360

Query: 361 LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 420
           LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE
Sbjct: 361 LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 420

Query: 421 LVQSTGAPTT 431
           LVQSTGAPTT
Sbjct: 421 LVQSTGAPTT 430

[Close]

BLAST of Carg22156 vs. NCBI nr
Match: KAG6593177.1 (Peptide chain release factor APG3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 796.6 bits (2056), Expect = 1.1e-226
Identity = 416/430 (96.74%), Postives = 416/430 (96.74%), Query Frame = 0


Query: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60
           MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES
Sbjct: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60

Query: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120
           AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE
Sbjct: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120

Query: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN 300
           PQTETQGRVHTSTATVAIMPE          ADEVEVVIDPKDIELTTTRSGGAGGQNVN
Sbjct: 241 PQTETQGRVHTSTATVAIMPE----------ADEVEVVIDPKDIELTTTRSGGAGGQNVN 300

Query: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV 360
           KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK   
Sbjct: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK--- 360

Query: 361 LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 420
            LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE
Sbjct: 361 -LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 416

Query: 421 LVQSTGAPTT 431
           LVQSTGAPTT
Sbjct: 421 LVQSTGAPTT 416

[Close]

BLAST of Carg22156 vs. NCBI nr
Match: XP_022959786.1 (peptide chain release factor APG3, chloroplastic [Cucurbita moschata] >XP_023004558.1 peptide chain release factor APG3, chloroplastic [Cucurbita maxima])

HSP 1 Score: 795.4 bits (2053), Expect = 2.4e-226
Identity = 415/430 (96.51%), Postives = 416/430 (96.74%), Query Frame = 0


Query: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60
           MNFFSPTTA+RTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES
Sbjct: 1   MNFFSPTTATRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60

Query: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120
           AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE
Sbjct: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120

Query: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN 300
           PQTETQGRVHTSTATVAIMPE          ADEVEVVIDPKDIELTTTRSGGAGGQNVN
Sbjct: 241 PQTETQGRVHTSTATVAIMPE----------ADEVEVVIDPKDIELTTTRSGGAGGQNVN 300

Query: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV 360
           KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK   
Sbjct: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK--- 360

Query: 361 LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 420
            LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE
Sbjct: 361 -LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 416

Query: 421 LVQSTGAPTT 431
           LVQSTGAPTT
Sbjct: 421 LVQSTGAPTT 416

[Close]

BLAST of Carg22156 vs. NCBI nr
Match: XP_023514061.1 (peptide chain release factor APG3, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 793.1 bits (2047), Expect = 1.2e-225
Identity = 413/430 (96.05%), Postives = 416/430 (96.74%), Query Frame = 0


Query: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60
           MNFFSPTTA+RTIPGCFLFPPRYGDFSRRCSL+LKRSAPPFRASTLVCMAEPYLITKLES
Sbjct: 1   MNFFSPTTATRTIPGCFLFPPRYGDFSRRCSLDLKRSAPPFRASTLVCMAEPYLITKLES 60

Query: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120
           AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE
Sbjct: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120

Query: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN 300
           PQTETQGRVHTSTATVAIMPE          ADEVEVVIDPKDIELTTTRSGGAGGQNVN
Sbjct: 241 PQTETQGRVHTSTATVAIMPE----------ADEVEVVIDPKDIELTTTRSGGAGGQNVN 300

Query: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV 360
           KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK   
Sbjct: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK--- 360

Query: 361 LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 420
            LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACV+MEQKELMEE
Sbjct: 361 -LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVSMEQKELMEE 416

Query: 421 LVQSTGAPTT 431
           LVQSTGAPTT
Sbjct: 421 LVQSTGAPTT 416

[Close]

BLAST of Carg22156 vs. NCBI nr
Match: XP_022151413.1 (peptide chain release factor APG3, chloroplastic [Momordica charantia])

HSP 1 Score: 735.7 bits (1898), Expect = 2.3e-208
Identity = 383/430 (89.07%), Postives = 395/430 (91.86%), Query Frame = 0


Query: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60
           MN FSPT+A+RT PGCFL PPR+ DFSRRCSLE KRSAP +RAST++CMAEPYLI KLES
Sbjct: 1   MNIFSPTSAARTCPGCFLLPPRHSDFSRRCSLEFKRSAPLYRASTVICMAEPYLIVKLES 60

Query: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120
           AE TWKELSVKLADPDIVSNPTEYQK AQSMSELDEVVSTFR FKECEKQL+E KALAKE
Sbjct: 61  AEKTWKELSVKLADPDIVSNPTEYQKFAQSMSELDEVVSTFRNFKECEKQLEETKALAKE 120

Query: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMAEMIALEI+SLSSQLKD+EEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEINSLSSQLKDLEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGN VYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNCVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN 300
           PQTETQGRVHTSTATVAIMPE          ADEVEVVIDPKDIEL T RSGGAGGQNVN
Sbjct: 241 PQTETQGRVHTSTATVAIMPE----------ADEVEVVIDPKDIELQTARSGGAGGQNVN 300

Query: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV 360
           KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQE IRNQRK   
Sbjct: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQELIRNQRK--- 360

Query: 361 LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 420
            LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSF++GDIENAVQAC AMEQKEL+EE
Sbjct: 361 -LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIENAVQACAAMEQKELLEE 416

Query: 421 LVQSTGAPTT 431
           L QS  A  T
Sbjct: 421 LAQSAVASAT 416

[Close]

BLAST of Carg22156 vs. ExPASy Swiss-Prot
Match: Q8RX79 (Peptide chain release factor APG3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APG3 PE=2 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 1.8e-168
Identity = 306/386 (79.27%), Postives = 344/386 (89.12%), Query Frame = 0


Query: 42  RASTLVCMAEPYLITKLESAENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTF 101
           R   LVCMAEPYLI K+ES E TWKELSVKLADPD+VSN +EYQKLAQSMSELDEVV+ F
Sbjct: 45  RTRRLVCMAEPYLIRKMESVEKTWKELSVKLADPDVVSNQSEYQKLAQSMSELDEVVTVF 104

Query: 102 RRFKECEKQLQEAKALAKESGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDA 161
           RRFK+CEKQL E+K LAKE+G+DEDMAEMI  EI+SL+ +++++E++LKV+LLPSDPLDA
Sbjct: 105 RRFKDCEKQLLESKVLAKEAGDDEDMAEMIGSEINSLTKEIEELEKQLKVLLLPSDPLDA 164

Query: 162 RNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKG 221
           RNI+LEVRAGTGGDEA IW GDLVRMYQ+YSER+SWKFS +S SEAE GG+KT V+EIKG
Sbjct: 165 RNILLEVRAGTGGDEAAIWTGDLVRMYQRYSERSSWKFSMVSCSEAEHGGYKTCVMEIKG 224

Query: 222 NRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDP 281
           NRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPE          ADEVEVVIDP
Sbjct: 225 NRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPE----------ADEVEVVIDP 284

Query: 282 KDIELTTTRSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLY 341
           KDIELT+ RSGGAGGQNVNKVETAIDLFHKP+GIRIFCTEERTQ++NK RAFQLLRAKLY
Sbjct: 285 KDIELTSARSGGAGGQNVNKVETAIDLFHKPSGIRIFCTEERTQIRNKARAFQLLRAKLY 344

Query: 342 EIKVREQQERIRNQRKLQVLLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGD 401
           EIKVREQQE+IRN+RK     QVGTGAR+EKIRTYNYKD+RVTDHRLKMNF LT+F+DG 
Sbjct: 345 EIKVREQQEKIRNERK----SQVGTGARSEKIRTYNYKDSRVTDHRLKMNFALTTFLDGA 404

Query: 402 IENAVQACVAMEQKELMEELVQSTGA 428
           +E+AVQAC A+EQKELMEEL +S  A
Sbjct: 405 LEDAVQACAALEQKELMEELSESVAA 416

[Close]

BLAST of Carg22156 vs. ExPASy Swiss-Prot
Match: Q110D7 (Peptide chain release factor 1 OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=prfA PE=3 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 2.6e-127
Identity = 231/380 (60.79%), Postives = 295/380 (77.63%), Query Frame = 0


Query: 49  MAEPYLITKLESAENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECE 108
           MAE YL+ KL+S E T+ EL+++LADPD+  +P+E+QKLA++ S L+EVV+ +  +K  +
Sbjct: 1   MAETYLLDKLKSVEQTYNELTLRLADPDVAKDPSEFQKLAKARSSLEEVVNCYVEWKNAQ 60

Query: 109 KQLQEAKALAKESGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEV 168
           ++L +AK + KE+  D +M EM  +E+  L ++L+ +E ++K+ LLP DP D +NIMLE+
Sbjct: 61  EELADAKEILKEAVGDLEMQEMAKVEVEDLEAKLESLENQMKIALLPRDPNDDKNIMLEI 120

Query: 169 RAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKL 228
           RAGTGGDEA IWAGDLVRMY +YSE  SWK S +S S A+ GGFK  +LEIKG+ VYSKL
Sbjct: 121 RAGTGGDEASIWAGDLVRMYSRYSENQSWKVSLLSESLADMGGFKEAILEIKGDHVYSKL 180

Query: 229 KYESGVHRVQRVPQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTT 288
           K+E+GVHRVQRVP TE  GRVHTSTATVAIMPEV          D+VEV ID KDIEL+T
Sbjct: 181 KFEAGVHRVQRVPVTEAGGRVHTSTATVAIMPEV----------DDVEVEIDQKDIELST 240

Query: 289 TRSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQ 348
            RSGGAGGQNVNKVETA+DLFHKPTGIRIFCT+ER+QLQN+ RA Q+LRAKLYEIK++EQ
Sbjct: 241 ARSGGAGGQNVNKVETAVDLFHKPTGIRIFCTQERSQLQNRERAMQILRAKLYEIKLQEQ 300

Query: 349 QERIRNQRKLQVLLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQA 408
           Q  + + R+     QVGTG+R+EKIRTYNYKDNRVTDHRL  NF L   ++GDIEN +QA
Sbjct: 301 QAEVSSIRR----SQVGTGSRSEKIRTYNYKDNRVTDHRLNQNFSLVPLLEGDIENVIQA 360

Query: 409 CVAMEQKELMEELVQSTGAP 429
           C+  +Q+E ++EL  S+  P
Sbjct: 361 CITQDQQERLQELAASSSTP 366

[Close]

BLAST of Carg22156 vs. ExPASy Swiss-Prot
Match: B7KI17 (Peptide chain release factor 1 OS=Gloeothece citriformis (strain PCC 7424) OX=65393 GN=prfA PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.0e-126
Identity = 232/376 (61.70%), Postives = 293/376 (77.93%), Query Frame = 0


Query: 49  MAEPYLITKLESAENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECE 108
           MAE YL+ KL+S E T+KEL+ +LADPDI +NP E Q++A++ S L+E V+T+  +++ +
Sbjct: 1   MAEAYLLEKLKSVEQTYKELTRRLADPDIATNPDELQRVAKARSSLEETVNTYETWQKTK 60

Query: 109 KQLQEAKALAKESGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEV 168
           +++  A+ + KE+G D +M EM ALE+  L  QL+ +E KLKV+LLP DP D +NIMLE+
Sbjct: 61  EEITGARQILKEAGGDSEMREMAALEVEELEEQLEQLENKLKVLLLPRDPNDDKNIMLEI 120

Query: 169 RAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKL 228
           RAGTGGDEA IWAGDLVRMY +Y+E  +W    +S S A+ GGFK  +LEIKG++VYSKL
Sbjct: 121 RAGTGGDEASIWAGDLVRMYSRYAESQNWVVKLVSESLADMGGFKEAILEIKGDQVYSKL 180

Query: 229 KYESGVHRVQRVPQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTT 288
           K+E+GVHRVQRVP TE  GRVHTSTATVAIMPEV          DEVEV IDPKDIE++T
Sbjct: 181 KFEAGVHRVQRVPVTEAGGRVHTSTATVAIMPEV----------DEVEVQIDPKDIEVST 240

Query: 289 TRSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQ 348
            RSGGAGGQNVNKVETA+DLFHKPTGIRIFCTEER+QLQN+ RA Q+LRAKLYE+K+REQ
Sbjct: 241 ARSGGAGGQNVNKVETAVDLFHKPTGIRIFCTEERSQLQNRERAMQILRAKLYEMKLREQ 300

Query: 349 QERIRNQRKLQVLLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQA 408
           QE + + R+     QVGTG+R+EKIRTYNYKDNRVTDHRL  NF L   ++GDIE  +Q+
Sbjct: 301 QEAVSSMRR----SQVGTGSRSEKIRTYNYKDNRVTDHRLGQNFSLVGALEGDIEVIIQS 360

Query: 409 CVAMEQKELMEELVQS 425
           C+  +Q+E + EL  S
Sbjct: 361 CIGKDQQERLAELANS 362

[Close]

BLAST of Carg22156 vs. ExPASy Swiss-Prot
Match: Q8DMG9 (Peptide chain release factor 1 OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=prfA PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.7e-126
Identity = 230/373 (61.66%), Postives = 293/373 (78.55%), Query Frame = 0


Query: 49  MAEPYLITKLESAENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECE 108
           MAE YL+ KL + E T+ EL+ +LADPD+  NPTE+QK+A+S + L+E V+ +  ++   
Sbjct: 1   MAEAYLLEKLRTVEQTFTELTRRLADPDVAVNPTEFQKVARSRAALEETVNAYHEWQSLN 60

Query: 109 KQLQEAKALAKESGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEV 168
           +QL +A+ L KE+ N+ ++ +M   EI+ LS ++  +EE+LK++LLP DP D +NIMLE+
Sbjct: 61  QQLVDARQLHKEAANEPELRQMAEEEIAHLSHRIAQLEERLKILLLPRDPNDEKNIMLEI 120

Query: 169 RAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKL 228
           RAG GGDEAGIWAGDLVRMY +Y++   W+ + +S S AE GGFK  +LEIKG+RVYSKL
Sbjct: 121 RAGAGGDEAGIWAGDLVRMYSRYAKTQGWQVTLVSESLAEMGGFKEAILEIKGDRVYSKL 180

Query: 229 KYESGVHRVQRVPQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTT 288
           KYESGVHRVQRVP TE  GR+HTSTATVA+MPEV          DEVEV IDPKDIELTT
Sbjct: 181 KYESGVHRVQRVPATEASGRIHTSTATVAVMPEV----------DEVEVEIDPKDIELTT 240

Query: 289 TRSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQ 348
            RSGGAGGQNVNKVETA+DL+HKPTGIRIFCTEER+QL+NK RAFQ+LRAKLYEIK+REQ
Sbjct: 241 ARSGGAGGQNVNKVETAVDLYHKPTGIRIFCTEERSQLKNKERAFQILRAKLYEIKLREQ 300

Query: 349 QERIRNQRKLQVLLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQA 408
           QE I + R+     QVGTG R+EKIRTYNYKD+RVTDHRL  NF L+S ++GD+E  +QA
Sbjct: 301 QEAITSMRR----SQVGTGDRSEKIRTYNYKDDRVTDHRLGQNFSLSSVLEGDLEPIIQA 359

Query: 409 CVAMEQKELMEEL 422
           C++ +Q+E + +L
Sbjct: 361 CISRDQQEQLAQL 359

[Close]

BLAST of Carg22156 vs. ExPASy Swiss-Prot
Match: B0JYC5 (Peptide chain release factor 1 OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=prfA PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 6.5e-126
Identity = 227/373 (60.86%), Postives = 292/373 (78.28%), Query Frame = 0


Query: 49  MAEPYLITKLESAENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECE 108
           MAE YL+ KL+S E T+KEL+ +LADPD+  NP E  +LA+S + L+E V+T+  +++ +
Sbjct: 1   MAESYLLDKLQSVEQTYKELTRRLADPDVTGNPGELHRLAKSRASLEETVNTYEIWQKSQ 60

Query: 109 KQLQEAKALAKESGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEV 168
           + L  AK + KES +D ++ EM ALE++ L  ++  +E++L ++LLP DPLD +NIMLE+
Sbjct: 61  EDLIGAKQIFKESASDPELREMAALEVAELEEKIATLEDQLTILLLPRDPLDEKNIMLEI 120

Query: 169 RAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKL 228
           RAGTGGDEA IWAGDLVR+Y KY+E  +WK S +S S+A+ GGFK  +LEIKG+ VYSKL
Sbjct: 121 RAGTGGDEASIWAGDLVRLYSKYAETQNWKVSLLSESQADMGGFKEAILEIKGDNVYSKL 180

Query: 229 KYESGVHRVQRVPQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTT 288
           K+E+GVHRVQRVP TE  GRVHTSTATVAIMPEV          D+VEV IDPKDIELTT
Sbjct: 181 KFEAGVHRVQRVPLTEASGRVHTSTATVAIMPEV----------DDVEVHIDPKDIELTT 240

Query: 289 TRSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQ 348
            RSGGAGGQNVNKVETA+DL HKPTGIR+FCTEER+QLQN+ RA Q+LRAKLY++K++EQ
Sbjct: 241 ARSGGAGGQNVNKVETAVDLIHKPTGIRVFCTEERSQLQNRERAMQILRAKLYDMKLQEQ 300

Query: 349 QERIRNQRKLQVLLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQA 408
           QE + + R+     QVGTG+R+EKIRTYNYKDNR+TDHRL  NF L   +DGDIE  +Q+
Sbjct: 301 QEAVSSMRR----SQVGTGSRSEKIRTYNYKDNRLTDHRLNQNFSLDRILDGDIEEVIQS 359

Query: 409 CVAMEQKELMEEL 422
           C+A +Q+E + EL
Sbjct: 361 CIAKDQQEKLAEL 359

[Close]

BLAST of Carg22156 vs. ExPASy TrEMBL
Match: A0A6J1KZW8 (peptide chain release factor APG3, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111497823 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.2e-226
Identity = 415/430 (96.51%), Postives = 416/430 (96.74%), Query Frame = 0


Query: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60
           MNFFSPTTA+RTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES
Sbjct: 1   MNFFSPTTATRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60

Query: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120
           AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE
Sbjct: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120

Query: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN 300
           PQTETQGRVHTSTATVAIMPE          ADEVEVVIDPKDIELTTTRSGGAGGQNVN
Sbjct: 241 PQTETQGRVHTSTATVAIMPE----------ADEVEVVIDPKDIELTTTRSGGAGGQNVN 300

Query: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV 360
           KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK   
Sbjct: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK--- 360

Query: 361 LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 420
            LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE
Sbjct: 361 -LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 416

Query: 421 LVQSTGAPTT 431
           LVQSTGAPTT
Sbjct: 421 LVQSTGAPTT 416

[Close]

BLAST of Carg22156 vs. ExPASy TrEMBL
Match: A0A6J1H5U4 (peptide chain release factor APG3, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460751 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.2e-226
Identity = 415/430 (96.51%), Postives = 416/430 (96.74%), Query Frame = 0


Query: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60
           MNFFSPTTA+RTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES
Sbjct: 1   MNFFSPTTATRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60

Query: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120
           AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE
Sbjct: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120

Query: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN 300
           PQTETQGRVHTSTATVAIMPE          ADEVEVVIDPKDIELTTTRSGGAGGQNVN
Sbjct: 241 PQTETQGRVHTSTATVAIMPE----------ADEVEVVIDPKDIELTTTRSGGAGGQNVN 300

Query: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV 360
           KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK   
Sbjct: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK--- 360

Query: 361 LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 420
            LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE
Sbjct: 361 -LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 416

Query: 421 LVQSTGAPTT 431
           LVQSTGAPTT
Sbjct: 421 LVQSTGAPTT 416

[Close]

BLAST of Carg22156 vs. ExPASy TrEMBL
Match: A0A6J1DC40 (peptide chain release factor APG3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019348 PE=3 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 1.1e-208
Identity = 383/430 (89.07%), Postives = 395/430 (91.86%), Query Frame = 0


Query: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60
           MN FSPT+A+RT PGCFL PPR+ DFSRRCSLE KRSAP +RAST++CMAEPYLI KLES
Sbjct: 1   MNIFSPTSAARTCPGCFLLPPRHSDFSRRCSLEFKRSAPLYRASTVICMAEPYLIVKLES 60

Query: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120
           AE TWKELSVKLADPDIVSNPTEYQK AQSMSELDEVVSTFR FKECEKQL+E KALAKE
Sbjct: 61  AEKTWKELSVKLADPDIVSNPTEYQKFAQSMSELDEVVSTFRNFKECEKQLEETKALAKE 120

Query: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMAEMIALEI+SLSSQLKD+EEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEINSLSSQLKDLEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGN VYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNCVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN 300
           PQTETQGRVHTSTATVAIMPE          ADEVEVVIDPKDIEL T RSGGAGGQNVN
Sbjct: 241 PQTETQGRVHTSTATVAIMPE----------ADEVEVVIDPKDIELQTARSGGAGGQNVN 300

Query: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV 360
           KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQE IRNQRK   
Sbjct: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQELIRNQRK--- 360

Query: 361 LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 420
            LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSF++GDIENAVQAC AMEQKEL+EE
Sbjct: 361 -LQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIENAVQACAAMEQKELLEE 416

Query: 421 LVQSTGAPTT 431
           L QS  A  T
Sbjct: 421 LAQSAVASAT 416

[Close]

BLAST of Carg22156 vs. ExPASy TrEMBL
Match: A0A5A7VLD6 (Peptide chain release factor APG3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00120 PE=3 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 7.3e-205
Identity = 375/430 (87.21%), Postives = 393/430 (91.40%), Query Frame = 0


Query: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60
           MN F+PTTA+ TIP  FL PP +  F +RCSLELKR APP RASTL+CMAEPYLI KLES
Sbjct: 1   MNIFTPTTAAPTIPSSFLLPPPHSGFFKRCSLELKRFAPPSRASTLICMAEPYLIVKLES 60

Query: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120
           AE TWKELSVKLADPD+VSNPTEYQKLAQSMSELDEVVSTFR+FKECE+QL+EAKAL+KE
Sbjct: 61  AEKTWKELSVKLADPDVVSNPTEYQKLAQSMSELDEVVSTFRKFKECERQLEEAKALSKE 120

Query: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDM EMIALEISSLSS+LKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSP+SSSEAEKGG+KTYVLEIKG RVYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPVSSSEAEKGGYKTYVLEIKGQRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN 300
           PQTETQGRVHTSTATVAIMPE          ADEVE+VIDPKDIELTT RSGGAGGQNVN
Sbjct: 241 PQTETQGRVHTSTATVAIMPE----------ADEVEIVIDPKDIELTTARSGGAGGQNVN 300

Query: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV 360
           KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQE+IRNQRK   
Sbjct: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQEKIRNQRK--- 360

Query: 361 LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 420
            LQVGTGARAEKIRTYNYKDNRVTDHRLK NFELTSF+DGDIE+AVQAC AMEQKEL+EE
Sbjct: 361 -LQVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLDGDIEDAVQACTAMEQKELLEE 416

Query: 421 LVQSTGAPTT 431
           L QS  A  T
Sbjct: 421 LAQSAVASAT 416

[Close]

BLAST of Carg22156 vs. ExPASy TrEMBL
Match: A0A1S3BRZ7 (peptide chain release factor APG3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492855 PE=3 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 7.3e-205
Identity = 375/430 (87.21%), Postives = 393/430 (91.40%), Query Frame = 0


Query: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60
           MN F+PTTA+ TIP  FL PP +  F +RCSLELKR APP RASTL+CMAEPYLI KLES
Sbjct: 1   MNIFTPTTAAPTIPSSFLLPPPHSGFFKRCSLELKRFAPPSRASTLICMAEPYLIVKLES 60

Query: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120
           AE TWKELSVKLADPD+VSNPTEYQKLAQSMSELDEVVSTFR+FKECE+QL+EAKAL+KE
Sbjct: 61  AEKTWKELSVKLADPDVVSNPTEYQKLAQSMSELDEVVSTFRKFKECERQLEEAKALSKE 120

Query: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDM EMIALEISSLSS+LKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSP+SSSEAEKGG+KTYVLEIKG RVYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPVSSSEAEKGGYKTYVLEIKGQRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVN 300
           PQTETQGRVHTSTATVAIMPE          ADEVE+VIDPKDIELTT RSGGAGGQNVN
Sbjct: 241 PQTETQGRVHTSTATVAIMPE----------ADEVEIVIDPKDIELTTARSGGAGGQNVN 300

Query: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQV 360
           KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQE+IRNQRK   
Sbjct: 301 KVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQEKIRNQRK--- 360

Query: 361 LLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEE 420
            LQVGTGARAEKIRTYNYKDNRVTDHRLK NFELTSF+DGDIE+AVQAC AMEQKEL+EE
Sbjct: 361 -LQVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLDGDIEDAVQACTAMEQKELLEE 416

Query: 421 LVQSTGAPTT 431
           L QS  A  T
Sbjct: 421 LAQSAVASAT 416

[Close]

BLAST of Carg22156 vs. TAIR 10
Match: AT3G62910.1 (Peptide chain release factor 1 )

HSP 1 Score: 593.6 bits (1529), Expect = 1.3e-169
Identity = 306/386 (79.27%), Postives = 344/386 (89.12%), Query Frame = 0


Query: 42  RASTLVCMAEPYLITKLESAENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTF 101
           R   LVCMAEPYLI K+ES E TWKELSVKLADPD+VSN +EYQKLAQSMSELDEVV+ F
Sbjct: 45  RTRRLVCMAEPYLIRKMESVEKTWKELSVKLADPDVVSNQSEYQKLAQSMSELDEVVTVF 104

Query: 102 RRFKECEKQLQEAKALAKESGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDA 161
           RRFK+CEKQL E+K LAKE+G+DEDMAEMI  EI+SL+ +++++E++LKV+LLPSDPLDA
Sbjct: 105 RRFKDCEKQLLESKVLAKEAGDDEDMAEMIGSEINSLTKEIEELEKQLKVLLLPSDPLDA 164

Query: 162 RNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKG 221
           RNI+LEVRAGTGGDEA IW GDLVRMYQ+YSER+SWKFS +S SEAE GG+KT V+EIKG
Sbjct: 165 RNILLEVRAGTGGDEAAIWTGDLVRMYQRYSERSSWKFSMVSCSEAEHGGYKTCVMEIKG 224

Query: 222 NRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDP 281
           NRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPE          ADEVEVVIDP
Sbjct: 225 NRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPE----------ADEVEVVIDP 284

Query: 282 KDIELTTTRSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLY 341
           KDIELT+ RSGGAGGQNVNKVETAIDLFHKP+GIRIFCTEERTQ++NK RAFQLLRAKLY
Sbjct: 285 KDIELTSARSGGAGGQNVNKVETAIDLFHKPSGIRIFCTEERTQIRNKARAFQLLRAKLY 344

Query: 342 EIKVREQQERIRNQRKLQVLLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGD 401
           EIKVREQQE+IRN+RK     QVGTGAR+EKIRTYNYKD+RVTDHRLKMNF LT+F+DG 
Sbjct: 345 EIKVREQQEKIRNERK----SQVGTGARSEKIRTYNYKDSRVTDHRLKMNFALTTFLDGA 404

Query: 402 IENAVQACVAMEQKELMEELVQSTGA 428
           +E+AVQAC A+EQKELMEEL +S  A
Sbjct: 405 LEDAVQACAALEQKELMEELSESVAA 416

[Close]

BLAST of Carg22156 vs. TAIR 10
Match: AT2G47020.1 (Peptide chain release factor 1 )

HSP 1 Score: 224.2 bits (570), Expect = 2.1e-58
Identity = 120/309 (38.83%), Postives = 192/309 (62.14%), Query Frame = 0


Query: 80  NPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKESGNDEDMAEMIALEISSLS 139
           +P E+ +  + + +L + +      +  +K++   K+L  ES +D+DM ++   E+    
Sbjct: 80  SPEEFSRANKELRKLRDSMLLINDLRAKQKEIDGLKSLVSESSDDKDMLDLAVGELDEAV 139

Query: 140 SQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKF 199
            + K ++  L   LLP D  D R+ +LEVRAGTGG+EA ++A D+ RMY++YS++  WKF
Sbjct: 140 EEEKRLQTLLLKSLLPKDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKKGWKF 199

Query: 200 SPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIM 259
             +  +E++  G+K     I G  VY KLK+ESG+HRVQR+P TE  GR+HTS  +VAI+
Sbjct: 200 DIVDITESDMKGYKEASAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAISVAIL 259

Query: 260 PEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVNKVETAIDLFHKPTGIRIFC 319
           P+          ADEV+V +  +D+ + T RSGG+GGQ+ N   +A+ + H PTG+ +  
Sbjct: 260 PQ----------ADEVDVQLRNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSI 319

Query: 320 TEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQVLLQVGTGARAEKIRTYNYK 379
            +ER+Q  N+ +A ++L A+LYEI    ++ RI++ R      Q+G+G R+ +IRTYN+ 
Sbjct: 320 QDERSQHMNRAKALKVLCARLYEI----ERLRIQSSRSKLRSDQIGSGDRSGRIRTYNFP 374

Query: 380 DNRVTDHRL 389
             RVTDHR+
Sbjct: 380 QGRVTDHRV 374

[Close]

BLAST of Carg22156 vs. TAIR 10
Match: AT2G47020.2 (Peptide chain release factor 1 )

HSP 1 Score: 184.1 bits (466), Expect = 2.4e-46
Identity = 94/246 (38.21%), Postives = 151/246 (61.38%), Query Frame = 0


Query: 80  NPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKESGNDEDMAEMIALEISSLS 139
           +P E+ +  + + +L + +      +  +K++   K+L  ES +D+DM ++   E+    
Sbjct: 15  SPEEFSRANKELRKLRDSMLLINDLRAKQKEIDGLKSLVSESSDDKDMLDLAVGELDEAV 74

Query: 140 SQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKF 199
            + K ++  L   LLP D  D R+ +LEVRAGTGG+EA ++A D+ RMY++YS++  WKF
Sbjct: 75  EEEKRLQTLLLKSLLPKDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKKGWKF 134

Query: 200 SPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIM 259
             +  +E++  G+K     I G  VY KLK+ESG+HRVQR+P TE  GR+HTS  +VAI+
Sbjct: 135 DIVDITESDMKGYKEASAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAISVAIL 194

Query: 260 PEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQNVNKVETAIDLFHKPTGIRIFC 319
           P+          ADEV+V +  +D+ + T RSGG+GGQ+ N   +A+ + H PTG+ +  
Sbjct: 195 PQ----------ADEVDVQLRNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSI 250

Query: 320 TEERTQ 326
            +ER+Q
Sbjct: 255 QDERSQ 250

[Close]

BLAST of Carg22156 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 159.1 bits (401), Expect = 8.1e-39
Identity = 107/351 (30.48%), Postives = 175/351 (49.86%), Query Frame = 0


Query: 58  LESAENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKAL 117
           L+  E     L  K  D     + T+ Q   +++S L+++    R   E +  +++A+ +
Sbjct: 104 LQQLEQEITNLESKATDTSFWDDRTKAQ---ETLSSLNDLKDRMRLLSEFKTMVEDAETI 163

Query: 118 AKESGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEA 177
            K +   +     +  E   +  +L    +K ++  L S P D    ++ + AG GG +A
Sbjct: 164 VKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDA 223

Query: 178 GIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRV 237
             WA  L+RMY ++ E+  +K   +  S  E+ G K+  LEI+G   Y  +  E G HR+
Sbjct: 224 QDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRI 283

Query: 238 QRVPQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQ 297
            R     ++G   TS + V +MP        LL  + V + I  +D++++ TR+GG GGQ
Sbjct: 284 VRQSPFNSKGLRQTSFSGVEVMP--------LLPEEAVGIEIPEEDLDISFTRAGGKGGQ 343

Query: 298 NVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK 357
           NVNKVETA+ + H PTG+ + CTEER+QL NK RA   L+AKL  I   ++   I+  R 
Sbjct: 344 NVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIRG 403

Query: 358 LQVLLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQA 409
             V  + G   R      Y    +  T H      ++TS +DGD++  ++A
Sbjct: 404 DAVKAEWGQQIRNYVFHPYKLVKDVRTGHETS---DITSVMDGDLDPFIKA 440

[Close]

BLAST of Carg22156 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 159.1 bits (401), Expect = 8.1e-39
Identity = 107/351 (30.48%), Postives = 175/351 (49.86%), Query Frame = 0


Query: 58  LESAENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKAL 117
           L+  E     L  K  D     + T+ Q   +++S L+++    R   E +  +++A+ +
Sbjct: 103 LQQLEQEITNLESKATDTSFWDDRTKAQ---ETLSSLNDLKDRMRLLSEFKTMVEDAETI 162

Query: 118 AKESGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEA 177
            K +   +     +  E   +  +L    +K ++  L S P D    ++ + AG GG +A
Sbjct: 163 VKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDA 222

Query: 178 GIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRV 237
             WA  L+RMY ++ E+  +K   +  S  E+ G K+  LEI+G   Y  +  E G HR+
Sbjct: 223 QDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRI 282

Query: 238 QRVPQTETQGRVHTSTATVAIMPEVNNFLNFLLNADEVEVVIDPKDIELTTTRSGGAGGQ 297
            R     ++G   TS + V +MP        LL  + V + I  +D++++ TR+GG GGQ
Sbjct: 283 VRQSPFNSKGLRQTSFSGVEVMP--------LLPEEAVGIEIPEEDLDISFTRAGGKGGQ 342

Query: 298 NVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRK 357
           NVNKVETA+ + H PTG+ + CTEER+QL NK RA   L+AKL  I   ++   I+  R 
Sbjct: 343 NVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIRG 402

Query: 358 LQVLLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQA 409
             V  + G   R      Y    +  T H      ++TS +DGD++  ++A
Sbjct: 403 DAVKAEWGQQIRNYVFHPYKLVKDVRTGHETS---DITSVMDGDLDPFIKA 439

The following BLAST results are available for this feature:

BLAST of Carg22156 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025567.1	9.4e-239	100.00	Peptide chain release factor APG3, chloroplastic [Cucurbita argyrosperma subsp. ...	[more]
KAG6593177.1	1.1e-226	96.74	Peptide chain release factor APG3, chloroplastic, partial [Cucurbita argyrosperm...	[more]
XP_022959786.1	2.4e-226	96.51	peptide chain release factor APG3, chloroplastic [Cucurbita moschata] >XP_023004...	[more]
XP_023514061.1	1.2e-225	96.05	peptide chain release factor APG3, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022151413.1	2.3e-208	89.07	peptide chain release factor APG3, chloroplastic [Momordica charantia]	[more]

BLAST of Carg22156 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RX79	1.8e-168	79.27	Peptide chain release factor APG3, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q110D7	2.6e-127	60.79	Peptide chain release factor 1 OS=Trichodesmium erythraeum (strain IMS101) OX=20...	[more]
B7KI17	1.0e-126	61.70	Peptide chain release factor 1 OS=Gloeothece citriformis (strain PCC 7424) OX=65...	[more]
Q8DMG9	1.7e-126	61.66	Peptide chain release factor 1 OS=Thermosynechococcus elongatus (strain BP-1) OX...	[more]
B0JYC5	6.5e-126	60.86	Peptide chain release factor 1 OS=Microcystis aeruginosa (strain NIES-843) OX=44...	[more]

BLAST of Carg22156 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KZW8	1.2e-226	96.51	peptide chain release factor APG3, chloroplastic OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1H5U4	1.2e-226	96.51	peptide chain release factor APG3, chloroplastic OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1DC40	1.1e-208	89.07	peptide chain release factor APG3, chloroplastic OS=Momordica charantia OX=3673 ...	[more]
A0A5A7VLD6	7.3e-205	87.21	Peptide chain release factor APG3 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3BRZ7	7.3e-205	87.21	peptide chain release factor APG3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of Carg22156 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62910.1	1.3e-169	79.27	Peptide chain release factor 1	[more]
AT2G47020.1	2.1e-58	38.83	Peptide chain release factor 1	[more]
AT2G47020.2	2.4e-46	38.21	Peptide chain release factor 1	[more]
AT5G36170.1	8.1e-39	30.48	high chlorophyll fluorescent 109	[more]
AT5G36170.2	8.1e-39	30.48	high chlorophyll fluorescent 109	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 338..358
None	No IPR available	COILS	Coil	Coil	coord: 128..148
None	No IPR available	COILS	Coil	Coil	coord: 104..124
None	No IPR available	GENE3D	3.30.70.1660		coord: 160..276 e-value: 3.7E-45 score: 155.7
None	No IPR available	GENE3D	3.30.160.20		coord: 279..339 e-value: 4.4E-24 score: 86.1
None	No IPR available	GENE3D	3.30.70.1660		coord: 340..424 e-value: 1.9E-9 score: 39.5
None	No IPR available	GENE3D	6.10.140.1950		coord: 48..158 e-value: 1.4E-28 score: 101.3
None	No IPR available	PANTHER	PTHR43804	LD18447P	coord: 39..428
None	No IPR available	PANTHER	PTHR43804:SF4	PEPTIDE CHAIN RELEASE FACTOR APG3, CHLOROPLASTIC	coord: 39..428
None	No IPR available	SUPERFAMILY	75620	Release factor	coord: 63..418
IPR005139	Peptide chain release factor	SMART	SM00937	PCRF_a_2	coord: 116..231 e-value: 4.8E-54 score: 195.6
IPR005139	Peptide chain release factor	PFAM	PF03462	PCRF	coord: 62..256 e-value: 4.7E-69 score: 232.1
IPR000352	Peptide chain release factor class I	PFAM	PF00472	RF-1	coord: 273..387 e-value: 3.9E-35 score: 120.3
IPR000352	Peptide chain release factor class I	PROSITE	PS00745	RF_PROK_I	coord: 290..306
IPR004373	Peptide chain release factor 1	TIGRFAM	TIGR00019	TIGR00019	coord: 50..422 e-value: 1.0E-136 score: 453.5
IPR004373	Peptide chain release factor 1	HAMAP	MF_00093	Rel_fac_1	coord: 54..422 score: 33.902618

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg22156-RA	Carg22156-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009658	chloroplast organization
biological_process	GO:0032544	plastid translation
biological_process	GO:0010027	thylakoid membrane organization
biological_process	GO:0006415	translational termination
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0016149	translation release factor activity, codon specific
molecular_function	GO:0003747	translation release factor activity